                         SEQUENCE LISTING

<110>  The University of Sheffield
 
<120>  Purification of Proteins

<130>  P521497PC00

<150>  GB2019607.7
<151>  2020-12-11

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic binding sequence

<400>  1

His Asn His Lys Ser Lys Lys His Lys 
1               5                   


<210>  2
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  2

Ala His Met Tyr Thr Lys Ala Gln Thr 
1               5                   


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  3

Tyr Val Lys Ser Thr Lys Lys Lys 
1               5               


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  4

Asn Trp Glu Lys Met Val Thr Tyr Lys 
1               5                   


<210>  5
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  5

Gln Arg Ala Gln Ser Val Ser Lys Lys 
1               5                   


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  6

Ile His His Ile His His Lys His Thr 
1               5                   


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  7

Gly Ser Tyr Lys Lys Lys Lys His Asn 
1               5                   


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  8

Pro Ala Gly Lys Lys His Lys Pro Ala 
1               5                   


<210>  9
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  9

Ile Ser Lys Lys Lys Thr Phe Tyr Val 
1               5                   


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  10

Gln Met Lys Lys Ser Lys Met Trp Lys 
1               5                   


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  11

Ile Tyr Lys Lys Lys Thr Leu Arg Leu 
1               5                   


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  12

Phe His Met Pro Leu Thr Asp Pro Gly Gln Val Gln 
1               5                   10          


<210>  13
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  13

Ala Ser His Thr Gly Thr Ala Gln Ile Ser Arg Gln 
1               5                   10          


<210>  14
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  14

His Ser Leu Pro His Gln Pro Pro Tyr Thr Thr Thr 
1               5                   10          


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  15

Asp Pro Pro Ile Lys Ser Val Arg Pro Ser Thr Thr 
1               5                   10          


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  16

Lys Ala Ala Leu Thr Ser Thr Ala Ile Pro Ser Leu 
1               5                   10          


<210>  17
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  17

Tyr Gln Gln Ala Ser Asp Asn Glu Leu Ser Tyr Leu 
1               5                   10          


<210>  18
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetic nanoparticle binding domain

<400>  18

Ala Ser Ala Val Thr Asp Ser Ile Ser Gln Tyr Pro 
1               5                   10          


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adhiron binding peptide

<400>  19

Gln Lys Phe Val Pro Lys Ser Thr Asn 
1               5                   


<210>  20
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Adhiron binding peptide

<400>  20

Pro Lys Lys Ser Lys Ile Glu Leu Lys 
1               5                   


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FePt binding peptide

<400>  21

Val Tyr Asn His Met Arg Gln 
1               5           


<210>  22
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FePt binding peptide

<400>  22

His Ala Arg Met Pro Trp Thr 
1               5           


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FePt binding peptide

<400>  23

Tyr Gln Gly Ala Ser Glu Asn 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FePt binding peptide

<400>  24

Glu Met Ser His Phe Ile Ala 
1               5           


<210>  25
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoPt binding peptide

<400>  25

Lys Thr His Glu Ile His Ser Pro Leu Leu His Lys 
1               5                   10          


<210>  26
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoPt binding peptide

<400>  26

His Asn Lys His Leu Pro Ser Thr Gln Pro Leu Ala 
1               5                   10          


<210>  27
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoPt binding peptide

<400>  27

Lys Ser Leu Ser Arg His Asp His Ile His His His 
1               5                   10          


<210>  28
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoPt binding peptide

<400>  28

Ser Val Ser Val Gly Met Lys Pro Ser Pro Arg Pro 
1               5                   10          


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CoPt binding peptide

<400>  29

Val Ile Ser Asn His Arg Glu Ser Ser Arg Pro Leu 
1               5                   10          


<210>  30
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  30
atggtaggtc tcaaaatgaa aaagatttgg ttggctctgg ctggtc                      46


<210>  31
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  31
atggtaggtc tcagcgctcg cggccgcagc gtcac                                  35


<210>  32
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer

<400>  32
cgcagttcga aaaatgctaa taagcttgat cc                                     32


<210>  33
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer

<400>  33
ggatcaagct tattagcatt tttcgaactg cg                                     32


<210>  34
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Binding peptide sequence

<400>  34

Gln Leu Arg Arg Asn Pro Glu Ala His 
1               5                   


<210>  35
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Magnetite nanoparticle binding protein

<400>  35

Met Gly Ser His His His His His His His His Gly Ser Thr Glu Asn 
1               5                   10                  15      


Leu Tyr Phe Gln Gly Pro Ser Met Lys Gln Leu Glu Lys Glu Leu Lys 
            20                  25                  30          


Gln Leu Glu Lys Glu Leu Gln Ala Ile Glu Lys Gln Leu Ala Gln Leu 
        35                  40                  45              


Gln Trp Lys Ala Gln Ala Arg Lys Lys Lys Leu Ala Gln Leu Lys Lys 
    50                  55                  60                  


Lys Leu Gln Ala Ala His Met Tyr Thr Lys Ala Gln Thr Gly Met Lys 
65                  70                  75                  80  


Gln Leu Glu Lys Glu Leu Lys Gln Leu Glu Lys Glu Leu Gln Ala Ile 
                85                  90                  95      


Glu Lys Gln Leu Ala Gln Leu Gln Trp Lys Ala Gln Ala Arg Lys Lys 
            100                 105                 110         


Lys Leu Ala Gln Leu Lys Lys Lys Leu Gln Ala 
        115                 120             


<210>  36
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Monobody scaffold

<400>  36

Met Gly Ser Ser His His His His His His Gly Gly Val Pro Arg Asp 
1               5                   10                  15      


Leu Glu Val Val Ala Ala Thr Pro Thr Ser Leu Leu Ile Ser Trp Asp 
            20                  25                  30          


Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr 
        35                  40                  45              


Gly Gly Asn Ser Pro Val Gln Glu Phe Thr Val Pro Gly Ser Lys Ser 
    50                  55                  60                  


Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly Val Asp Tyr Thr Ile Thr 
65                  70                  75                  80  


Val Tyr Ala Val Thr Gly Ala His Met Tyr Thr Lys Ala Gln Thr Pro 
                85                  90                  95      


Ile Ser Ile Asn Tyr Arg Thr Glu Ile Asp 
            100                 105     


<210>  37
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Monobody scaffold containing control sequence

<400>  37

Met Gly Ser Ser His His His His His His Gly Gly Val Pro Arg Asp 
1               5                   10                  15      


Leu Glu Val Val Ala Ala Thr Pro Thr Ser Leu Leu Ile Ser Trp Asp 
            20                  25                  30          


Ala Pro Ala Val Thr Val Arg Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr 
        35                  40                  45              


Gly Gly Asn Ser Pro Val Gln Glu Phe Thr Val Pro Gly Ser Lys Ser 
    50                  55                  60                  


Thr Ala Thr Ile Ser Gly Leu Lys Pro Gly Val Asp Tyr Thr Ile Thr 
65                  70                  75                  80  


Val Tyr Ala Val Thr Gly Asp Trp Trp Glu Ala Gly Val Phe Met Pro 
                85                  90                  95      


Ile Ser Ile Asn Tyr Arg Thr Glu Ile Asp 
            100                 105     


<210>  38
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable loop region

<400>  38

Asp Arg Asp Asp Glu Phe Val Tyr Phe His Ala 
1               5                   10      


<210>  39
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable loop region

<400>  39

Asn Val Arg Leu Leu Lys Glu Lys Arg Ile Thr Asn Thr Glu Ala Ala 
1               5                   10                  15      


Ile Asp Thr Gly Lys Glu Thr Val Gly Ala Gly Leu Ala Thr 
            20                  25                  30  


<210>  40
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable loop region

<400>  40

Ser Arg Asp Asp Glu Tyr Val Tyr Phe His Ala 
1               5                   10      


<210>  41
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable loop region

<400>  41

Asn Lys Asp Asp Glu Tyr Val Gln Phe His Ala 
1               5                   10      


<210>  42
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variable region

<400>  42

Glu Asn Val Glu Arg Val Met Ala Glu Glu Gly Leu Pro Pro Lys Glu 
1               5                   10                  15      


Ala Thr Arg Lys Ser Met Gly Gln Leu 
            20                  25  


<210>  43
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sequence

<400>  43

Met Lys Gln Leu Glu Lys Glu Leu Lys Gln Leu Glu Lys Glu Leu Gln 
1               5                   10                  15      


Ala Ile Glu Lys Gln Leu Ala Gln Leu Gln Trp Lys Ala Gln Ala Arg 
            20                  25                  30          


Lys Lys Lys Leu Ala Gln Leu Lys Lys Lys Leu Gln Ala 
        35                  40                  45  


<210>  44
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  PeIB

<400>  44

Lys Tyr Leu Leu Pro Thr Ala Ala Ala Gly Leu Leu Leu Leu Ala Ala 
1               5                   10                  15      


Gln Pro Ala Met Ala 
            20      


<210>  45
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Tag

<400>  45

Gly Ser Ser His His His His His His 
1               5                   


<210>  46
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TEV cleavage site

<400>  46

Glu Asn Leu Tyr Phe Gln Gly 
1               5           


<210>  47
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi-Tag

<400>  47

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  48
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nhel Site

<400>  48

Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu 
            20                  25                  30          


Gly Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp 
        35                  40                  45              


Pro Thr Leu Val Thr Thr Phe Ser Tyr Gly Val Gln Cys Phe Ser Arg 
    50                  55                  60                  


Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro 
65                  70                  75                  80  


Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn 
                85                  90                  95      


Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn 
            100                 105                 110         


Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu 
        115                 120                 125             


Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Met 
    130                 135                 140                 


Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe Lys Ile Arg His 
145                 150                 155                 160 


Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn 
                165                 170                 175     


Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu 
            180                 185                 190         


Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His 
        195                 200                 205             


Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met 
    210                 215                 220                 


Asp Glu Leu Tyr Lys 
225                 


<210>  49
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  49

Gly Gly Gly Gly Ser 
1               5   


<210>  50
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ADHA3 peptide

<400>  50

His Asn His Lys Ser Lys Lys His Lys 
1               5                   


<210>  51
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Protein fusion

<400>  51
aaatacctgc tgccgaccgc tgctgctggt ctgctgctcc tcgctgccca gccggcgatg       60

gccggtagca gccatcacca tcatcatcat gaaaacctgt acttccaggg tggtctgaac      120

gatatttttg aggcccagaa aattgaatgg catgaagcta gcatgagcaa aggtgaagaa      180

ctgtttaccg gtgttgttcc gattctggtt gaactggatg gtgacgttaa tggtcacaaa      240

ttttcagtta gcggtgaagg cgaaggtgat gcaacctatg gtaaactgac cctgaaattt      300

atctgtacca ccggcaaact gccggttccg tggccgacac tggttaccac ctttagctat      360

ggtgttcagt gttttagccg ttatccggat catatgaaac agcacgattt tttcaaaagc      420

gcaatgccgg aaggttatgt tcaagaacgt accatcttct tcaaagatga cggcaactat      480

aaaacccgtg ccgaagttaa atttgaaggt gataccctgg tgaatcgcat tgaactgaaa      540

ggcatcgatt ttaaagagga tggtaatatc ctgggccaca aactggaata taattataat      600

agccacaacg tgtacatcat ggccgacaaa cagaaaaatg gcatcaaagt gaacttcaag      660

atccgccata atattgaaga tggttcagtt cagctggccg atcattatca gcagaatacc      720

ccgattggtg atggtccggt tctgctgccg gataatcatt atctgagcac ccagagcgca      780

ctgagcaaag atccgaatga aaaacgtgat cacatggtgc tgctggaatt tgttaccgca      840

gcaggtatta cccatggtat ggatgaactg tataaagcgg ccgccggtgg tggtggtagc      900

cataatcata aaagcaaaaa gcataaa                                          927


