                         SEQUENCE LISTING

<110>  CEA COMMISSARIAT A L'ENERGIE ATOMIQUE
       LABORATOIRE FRANAIS DU FRACTIONNEMENT ET DES BIOTECHNOLOGIES
 
<120>  NOVEL HUMAN ERYTHROID PROGENITOR CELL LINE HIGHLY PERMISSIVE TO B19 INFECTION AND USES THEREOF

<130>  B380668PCT D40853

<150>  EP20306551.1
<151>  2021-12-11

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Sense primer B19-21

<400>  1
tggcagacca gtttcgtgaa                                                   20


<210>  2
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Antisense primer B19-22

<400>  2
ccggcaaact tccttgaaaa                                                   20


<210>  3
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  Probe B19-V23, with a VIC tag in 5'

<400>  3
cagctgcccc tgtggccc                                                     18


<210>  4
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>  Sense primer actin-S

<400>  4
ggcacccagc acaatgaag                                                    19


<210>  5
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  Antisense primer actin-AS

<400>  5
gccgatccac acggagtact                                                   20


<210>  6
<211>  26
<212>  DNA
<213>  Artificial

<220>
<223>  Probe actin-FAM, with a FAM tag in 5'

<400>  6
tcaagatcat tgctcctcct gagcgc                                            26


<210>  7
<211>  2151
<212>  DNA
<213>  Artificial

<220>
<223>  Fucci Sensor nucleic acid sequence

<400>  7
atggtcagca agggggaaga agacaatatg gctatcatca aagagtttat gcggttcaag       60

gtgcacatgg aaggaagcgt caatgggcac gagttcgaga tcgaaggaga gggagagggc      120

agaccctacg agggcaccca gacagccaag ctgaaggtga ccaagggagg accactgcct      180

ttcgcctggg acatcctgag cccacagttt atgtacggct ccaaggccta tgtgaagcac      240

cccgccgaca tccctgatta tctgaagctg tccttccccg agggctttaa gtgggagaga      300

gtgatgaatt ttgaggatgg cggcgtggtg accgtgacac aggacagctc cctgcaggat      360

ggcgagttca tctacaaggt gaagctgagg ggcaccaact ttccatctga cggccccgtg      420

atgcagaaga agacaatggg ctgggaggcc tctagcgagc gcatgtatcc tgaggatggc      480

gccctgaagg gcgagatcaa gcagcggctg aagctgaagg acggcggcca ctacgatgcc      540

gaggtgaaga ccacatataa ggccaagaag cctgtgcagc tgccaggcgc ctacaacgtg      600

aatatcaagc tggacatcac cagccacaac gaggattaca caatcgtgga gcagtatgag      660

agggcagagg gccgccactc caccggaggc atggacgagc tgtacaaggg ctatccatct      720

cactggcggc ccctggagcc aagcccagcc cggcctgccc tgagagcacc agcaagcgcc      780

acaagcggct ccagaaagag ggccagaccc cctgcagcac ctggaaggga tcaggcccgc      840

ccaccagcac ggagaaggct gaggctgtcc gtggacgagg tgtcctctcc atctacccca      900

gaggcacctg atatcccagc atgccctagc ccaggccaga agatcaagaa gtccacacca      960

gcagcaggac agcctccaca cctgacctcc gcccaggacc aggatacaat ccagctggag     1020

ggaaggggct ctctgctgac ctgtggcgac gtggaggaga atcccggccc tatggtgtcc     1080

aagggcgagg agctgttcac aggcgtggtg cccatcctgg tggagctgga cggcgatgtg     1140

aacggccaca agttttctgt gagcggagag ggagagggcg atgcaaccta cggcaagctg     1200

acactgaagc tgatctgcac cacaggcaag ctgccagtgc cctggcctac cctggtgacc     1260

acactgggct acggcctgca gtgtttcgcc cggtatcctg accacatgaa gcagcacgat     1320

ttctttaaga gcgccatgcc agagggctac gtgcaggagc ggaccatctt ctttaaggac     1380

gatggcaatt ataagaccag agccgaggtg aagttcgagg gcgacacact ggtgaacagg     1440

atcgagctga agggcatcga ctttaaggag gatggcaata tcctgggcca caagctggag     1500

tacaactata attcccacaa cgtgtacatc accgccgata agcagaagaa cggcatcaag     1560

gccaatttca agatcagaca caacatcgag gacggaggag tgcagctggc agatcactat     1620

cagcagaaca caccaatcgg cgacggaccc gtgctgctgc ctgataatca ctacctgtcc     1680

tatcagtcta agctgagcaa ggaccctaat gagaagcgcg atcacatggt gctgctggag     1740

tttgtgaccg cagcaggaat cacactggga atggatgaac tctacaaagg atatcccagc     1800

cactggagac ctcttgagat gaacccaagc atgaagcaga agcaggagga gatcaaggag     1860

aacatcaaga atagctccgt gccacgccgg accctgaaga tgatccagcc atccgcctct     1920

ggcagcctgg tgggaaggga gaacgagctg tccgccggcc tgtctaagcg gaagcacaga     1980

aatgaccacc tgacctctac cacatctagc cccggcgtga tcgtgcctga gtcctctgag     2040

aacaagaatc tgggcggcgt gacacaggag agctttgatc tgatgatcaa ggaaaaccca     2100

tcaagccagt attggaaaga ggtcgctgag aaaagaagaa aagccctgta a              2151


<210>  8
<211>  716
<212>  PRT
<213>  Artificial

<220>
<223>  Fucci Sensor Protein sequence

<400>  8

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys Gly Tyr Pro Ser 
225                 230                 235                 240 


His Trp Arg Pro Leu Glu Pro Ser Pro Ala Arg Pro Ala Leu Arg Ala 
                245                 250                 255     


Pro Ala Ser Ala Thr Ser Gly Ser Arg Lys Arg Ala Arg Pro Pro Ala 
            260                 265                 270         


Ala Pro Gly Arg Asp Gln Ala Arg Pro Pro Ala Arg Arg Arg Leu Arg 
        275                 280                 285             


Leu Ser Val Asp Glu Val Ser Ser Pro Ser Thr Pro Glu Ala Pro Asp 
    290                 295                 300                 


Ile Pro Ala Cys Pro Ser Pro Gly Gln Lys Ile Lys Lys Ser Thr Pro 
305                 310                 315                 320 


Ala Ala Gly Gln Pro Pro His Leu Thr Ser Ala Gln Asp Gln Asp Thr 
                325                 330                 335     


Ile Gln Leu Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
            340                 345                 350         


Glu Asn Pro Gly Pro Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly 
        355                 360                 365             


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
    370                 375                 380                 


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
385                 390                 395                 400 


Thr Leu Lys Leu Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
                405                 410                 415     


Thr Leu Val Thr Thr Leu Gly Tyr Gly Leu Gln Cys Phe Ala Arg Tyr 
            420                 425                 430         


Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu 
        435                 440                 445             


Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr 
    450                 455                 460                 


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
465                 470                 475                 480 


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
                485                 490                 495     


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala 
            500                 505                 510         


Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn 
        515                 520                 525             


Ile Glu Asp Gly Gly Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
    530                 535                 540                 


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
545                 550                 555                 560 


Tyr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
                565                 570                 575     


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp 
            580                 585                 590         


Glu Leu Tyr Lys Gly Tyr Pro Ser His Trp Arg Pro Leu Glu Met Asn 
        595                 600                 605             


Pro Ser Met Lys Gln Lys Gln Glu Glu Ile Lys Glu Asn Ile Lys Asn 
    610                 615                 620                 


Ser Ser Val Pro Arg Arg Thr Leu Lys Met Ile Gln Pro Ser Ala Ser 
625                 630                 635                 640 


Gly Ser Leu Val Gly Arg Glu Asn Glu Leu Ser Ala Gly Leu Ser Lys 
                645                 650                 655     


Arg Lys His Arg Asn Asp His Leu Thr Ser Thr Thr Ser Ser Pro Gly 
            660                 665                 670         


Val Ile Val Pro Glu Ser Ser Glu Asn Lys Asn Leu Gly Gly Val Thr 
        675                 680                 685             


Gln Glu Ser Phe Asp Leu Met Ile Lys Glu Asn Pro Ser Ser Gln Tyr 
    690                 695                 700                 


Trp Lys Glu Val Ala Glu Lys Arg Arg Lys Ala Leu 
705                 710                 715     


