                         SEQUENCE LISTING

<110>  GENETHON
 
<120>  LYSOSOMAL ACID LIPASE VARIANTS AND USES THEREOF

<130>  B3440PC00

<160>  33    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  PRT
<213>  artificial

<220>
<223>  Sp 1 : LAL WT signal peptide

<400>  1

Met Lys Met Arg Phe Leu Gly Leu Val Val Cys Leu Val Leu Trp Thr 
1               5                   10                  15      


Leu His Ser Glu Gly Ser Gly 
            20              


<210>  2
<211>  24
<212>  PRT
<213>  artificial

<220>
<223>  Sp 2 : alpha-1-antytripsin signal peptide

<400>  2

Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala 
            20                  


<210>  3
<211>  25
<212>  PRT
<213>  artificial

<220>
<223>  Sp 6 : iduronate 2-sulfatase signal peptide

<400>  3

Met Pro Pro Pro Arg Thr Gly Arg Gly Leu Leu Trp Leu Gly Leu Val 
1               5                   10                  15      


Leu Ser Ser Val Cys Val Ala Leu Gly 
            20                  25  


<210>  4
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  Sp 7 : chymotrypsinogen B2 signal peptide

<400>  4

Met Ala Phe Leu Trp Leu Leu Ser Cys Trp Ala Leu Leu Gly Thr Thr 
1               5                   10                  15      


Phe Gly 
        


<210>  5
<211>  22
<212>  PRT
<213>  artificial

<220>
<223>  Sp 8 : plasma protease inhibitor signal peptide

<400>  5

Met Ala Ser Arg Leu Thr Leu Leu Thr Leu Leu Leu Leu Leu Leu Ala 
1               5                   10                  15      


Gly Asp Arg Ala Ser Ser 
            20          


<210>  6
<211>  18
<212>  PRT
<213>  artificial

<220>
<223>  Sp 9 : serum albumin preproprotein signal peptide

<400>  6

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Leu Phe Ser Ser Ala 
1               5                   10                  15      


Tyr Ser 
        


<210>  7
<211>  19
<212>  PRT
<213>  artificial

<220>
<223>  Sp 10 : azurocidin preproprotein signal peptide

<400>  7

Met Thr Arg Leu Thr Val Leu Ala Leu Leu Ala Gly Leu Leu Ala Ser 
1               5                   10                  15      


Ser Arg Ala 
            


<210>  8
<211>  399
<212>  PRT
<213>  Homo sapiens

<400>  8

Met Lys Met Arg Phe Leu Gly Leu Val Val Cys Leu Val Leu Trp Thr 
1               5                   10                  15      


Leu His Ser Glu Gly Ser Gly Gly Lys Leu Thr Ala Val Asp Pro Glu 
            20                  25                  30          


Thr Asn Met Asn Val Ser Glu Ile Ile Ser Tyr Trp Gly Phe Pro Ser 
        35                  40                  45              


Glu Glu Tyr Leu Val Glu Thr Glu Asp Gly Tyr Ile Leu Cys Leu Asn 
    50                  55                  60                  


Arg Ile Pro His Gly Arg Lys Asn His Ser Asp Lys Gly Pro Lys Pro 
65                  70                  75                  80  


Val Val Phe Leu Gln His Gly Leu Leu Ala Asp Ser Ser Asn Trp Val 
                85                  90                  95      


Thr Asn Leu Ala Asn Ser Ser Leu Gly Phe Ile Leu Ala Asp Ala Gly 
            100                 105                 110         


Phe Asp Val Trp Met Gly Asn Ser Arg Gly Asn Thr Trp Ser Arg Lys 
        115                 120                 125             


His Lys Thr Leu Ser Val Ser Gln Asp Glu Phe Trp Ala Phe Ser Tyr 
    130                 135                 140                 


Asp Glu Met Ala Lys Tyr Asp Leu Pro Ala Ser Ile Asn Phe Ile Leu 
145                 150                 155                 160 


Asn Lys Thr Gly Gln Glu Gln Val Tyr Tyr Val Gly His Ser Gln Gly 
                165                 170                 175     


Thr Thr Ile Gly Phe Ile Ala Phe Ser Gln Ile Pro Glu Leu Ala Lys 
            180                 185                 190         


Arg Ile Lys Met Phe Phe Ala Leu Gly Pro Val Ala Ser Val Ala Phe 
        195                 200                 205             


Cys Thr Ser Pro Met Ala Lys Leu Gly Arg Leu Pro Asp His Leu Ile 
    210                 215                 220                 


Lys Asp Leu Phe Gly Asp Lys Glu Phe Leu Pro Gln Ser Ala Phe Leu 
225                 230                 235                 240 


Lys Trp Leu Gly Thr His Val Cys Thr His Val Ile Leu Lys Glu Leu 
                245                 250                 255     


Cys Gly Asn Leu Cys Phe Leu Leu Cys Gly Phe Asn Glu Arg Asn Leu 
            260                 265                 270         


Asn Met Ser Arg Val Asp Val Tyr Thr Thr His Ser Pro Ala Gly Thr 
        275                 280                 285             


Ser Val Gln Asn Met Leu His Trp Ser Gln Ala Val Lys Phe Gln Lys 
    290                 295                 300                 


Phe Gln Ala Phe Asp Trp Gly Ser Ser Ala Lys Asn Tyr Phe His Tyr 
305                 310                 315                 320 


Asn Gln Ser Tyr Pro Pro Thr Tyr Asn Val Lys Asp Met Leu Val Pro 
                325                 330                 335     


Thr Ala Val Trp Ser Gly Gly His Asp Trp Leu Ala Asp Val Tyr Asp 
            340                 345                 350         


Val Asn Ile Leu Leu Thr Gln Ile Thr Asn Leu Val Phe His Glu Ser 
        355                 360                 365             


Ile Pro Glu Trp Glu His Leu Asp Phe Ile Trp Gly Leu Asp Ala Pro 
    370                 375                 380                 


Trp Arg Leu Tyr Asn Lys Ile Ile Asn Leu Met Arg Lys Tyr Gln 
385                 390                 395                 


<210>  9
<211>  376
<212>  PRT
<213>  artificial

<220>
<223>  hLAL devoid of sp1

<400>  9

Gly Lys Leu Thr Ala Val Asp Pro Glu Thr Asn Met Asn Val Ser Glu 
1               5                   10                  15      


Ile Ile Ser Tyr Trp Gly Phe Pro Ser Glu Glu Tyr Leu Val Glu Thr 
            20                  25                  30          


Glu Asp Gly Tyr Ile Leu Cys Leu Asn Arg Ile Pro His Gly Arg Lys 
        35                  40                  45              


Asn His Ser Asp Lys Gly Pro Lys Pro Val Val Phe Leu Gln His Gly 
    50                  55                  60                  


Leu Leu Ala Asp Ser Ser Asn Trp Val Thr Asn Leu Ala Asn Ser Ser 
65                  70                  75                  80  


Leu Gly Phe Ile Leu Ala Asp Ala Gly Phe Asp Val Trp Met Gly Asn 
                85                  90                  95      


Ser Arg Gly Asn Thr Trp Ser Arg Lys His Lys Thr Leu Ser Val Ser 
            100                 105                 110         


Gln Asp Glu Phe Trp Ala Phe Ser Tyr Asp Glu Met Ala Lys Tyr Asp 
        115                 120                 125             


Leu Pro Ala Ser Ile Asn Phe Ile Leu Asn Lys Thr Gly Gln Glu Gln 
    130                 135                 140                 


Val Tyr Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile Ala 
145                 150                 155                 160 


Phe Ser Gln Ile Pro Glu Leu Ala Lys Arg Ile Lys Met Phe Phe Ala 
                165                 170                 175     


Leu Gly Pro Val Ala Ser Val Ala Phe Cys Thr Ser Pro Met Ala Lys 
            180                 185                 190         


Leu Gly Arg Leu Pro Asp His Leu Ile Lys Asp Leu Phe Gly Asp Lys 
        195                 200                 205             


Glu Phe Leu Pro Gln Ser Ala Phe Leu Lys Trp Leu Gly Thr His Val 
    210                 215                 220                 


Cys Thr His Val Ile Leu Lys Glu Leu Cys Gly Asn Leu Cys Phe Leu 
225                 230                 235                 240 


Leu Cys Gly Phe Asn Glu Arg Asn Leu Asn Met Ser Arg Val Asp Val 
                245                 250                 255     


Tyr Thr Thr His Ser Pro Ala Gly Thr Ser Val Gln Asn Met Leu His 
            260                 265                 270         


Trp Ser Gln Ala Val Lys Phe Gln Lys Phe Gln Ala Phe Asp Trp Gly 
        275                 280                 285             


Ser Ser Ala Lys Asn Tyr Phe His Tyr Asn Gln Ser Tyr Pro Pro Thr 
    290                 295                 300                 


Tyr Asn Val Lys Asp Met Leu Val Pro Thr Ala Val Trp Ser Gly Gly 
305                 310                 315                 320 


His Asp Trp Leu Ala Asp Val Tyr Asp Val Asn Ile Leu Leu Thr Gln 
                325                 330                 335     


Ile Thr Asn Leu Val Phe His Glu Ser Ile Pro Glu Trp Glu His Leu 
            340                 345                 350         


Asp Phe Ile Trp Gly Leu Asp Ala Pro Trp Arg Leu Tyr Asn Lys Ile 
        355                 360                 365             


Ile Asn Leu Met Arg Lys Tyr Gln 
    370                 375     


<210>  10
<211>  1200
<212>  DNA
<213>  Homo sapiens

<400>  10
atgaaaatgc ggttcttggg gttggtggtc tgtttggttc tctggaccct gcattctgag       60

gggtctggag ggaaactgac agctgtggat cctgaaacaa acatgaatgt gagtgaaatt      120

atctcttact ggggattccc tagtgaggaa tacctagttg agacagaaga tggatatatt      180

ctgtgcctta accgaattcc tcatgggagg aagaaccatt ctgacaaagg tcccaaacca      240

gttgtcttcc tgcaacatgg cttgctggca gattctagta actgggtcac aaaccttgcc      300

aacagcagcc tgggcttcat tcttgctgat gctggttttg acgtgtggat gggcaacagc      360

agaggaaata cctggtctcg gaaacataag acactctcag tttctcagga tgaattctgg      420

gctttcagtt atgatgagat ggcaaaatat gacctaccag cttccattaa cttcattctg      480

aataaaactg gccaagaaca agtgtattat gtgggtcatt ctcaaggcac cactataggt      540

tttatagcat tttcacagat ccctgagctg gctaaaagga ttaaaatgtt ttttgccctg      600

ggtcctgtgg cttccgtcgc cttctgtact agccctatgg ccaaattagg acgattacca      660

gatcatctca ttaaggactt atttggagac aaagaatttc ttccccagag tgcgtttttg      720

aagtggctgg gtacccacgt ttgcactcat gtcatactga aggagctctg tggaaatctc      780

tgttttcttc tgtgtggatt taatgagaga aatttaaata tgtctagagt ggatgtatat      840

acaacacatt ctcctgctgg aacttctgtg caaaacatgt tacactggag ccaggctgtt      900

aaattccaaa agtttcaagc ctttgactgg ggaagcagtg ccaagaatta ttttcattac      960

aaccagagtt atcctcccac atacaatgtg aaggacatgc ttgtgccgac tgcagtctgg     1020

agcgggggtc acgactggct tgcagatgtc tacgacgtca atatcttact gactcagatc     1080

accaacttgg tgttccatga gagcattccg gaatgggagc atcttgactt catttggggc     1140

ctggatgccc cttggaggct ttataataaa attattaatc taatgaggaa atatcagtaa     1200


<210>  11
<211>  75
<212>  DNA
<213>  artificial

<220>
<223>  Sp6 coding sequence

<400>  11
atgccgccac cccggaccgg ccgaggcctt ctctggctgg gtctggttct gagctccgtc       60

tgcgtcgccc tcgga                                                        75


<210>  12
<211>  54
<212>  DNA
<213>  artificial

<220>
<223>  Sp7 coding sequence

<400>  12
atggctttcc tctggctcct ctcctgctgg gccctcctgg gtaccacctt cggc             54


<210>  13
<211>  66
<212>  DNA
<213>  artificial

<220>
<223>  sp8 coding sequence

<400>  13
atggcctcca ggctgaccct gctgaccctc ctgctgctgc tgctggctgg ggatagagcc       60

tcctca                                                                  66


<210>  14
<211>  1131
<212>  DNA
<213>  artificial

<220>
<223>  WT hLAL w/o signal peptide

<400>  14
gggaaactga cagctgtgga tcctgaaaca aacatgaatg tgagtgaaat tatctcttac       60

tggggattcc ctagtgagga atacctagtt gagacagaag atggatatat tctgtgcctt      120

aaccgaattc ctcatgggag gaagaaccat tctgacaaag gtcccaaacc agttgtcttc      180

ctgcaacatg gcttgctggc agattctagt aactgggtca caaaccttgc caacagcagc      240

ctgggcttca ttcttgctga tgctggtttt gacgtgtgga tgggcaacag cagaggaaat      300

acctggtctc ggaaacataa gacactctca gtttctcagg atgaattctg ggctttcagt      360

tatgatgaga tggcaaaata tgacctacca gcttccatta acttcattct gaataaaact      420

ggccaagaac aagtgtatta tgtgggtcat tctcaaggca ccactatagg ttttatagca      480

ttttcacaga tccctgagct ggctaaaagg attaaaatgt tttttgccct gggtcctgtg      540

gcttccgtcg ccttctgtac tagccctatg gccaaattag gacgattacc agatcatctc      600

attaaggact tatttggaga caaagaattt cttccccaga gtgcgttttt gaagtggctg      660

ggtacccacg tttgcactca tgtcatactg aaggagctct gtggaaatct ctgttttctt      720

ctgtgtggat ttaatgagag aaatttaaat atgtctagag tggatgtata tacaacacat      780

tctcctgctg gaacttctgt gcaaaacatg ttacactgga gccaggctgt taaattccaa      840

aagtttcaag cctttgactg gggaagcagt gccaagaatt attttcatta caaccagagt      900

tatcctccca catacaatgt gaaggacatg cttgtgccga ctgcagtctg gagcgggggt      960

cacgactggc ttgcagatgt ctacgacgtc aatatcttac tgactcagat caccaacttg     1020

gtgttccatg agagcattcc ggaatgggag catcttgact tcatttgggg cctggatgcc     1080

ccttggaggc tttataataa aattattaat ctaatgagga aatatcagta a              1131


<210>  15
<211>  1131
<212>  DNA
<213>  artificial

<220>
<223>  hLAL w/o signal peptide (from Opt_1)

<400>  15
gggaagctca cggcagtaga cccggagacc aatatgaatg ttagcgagat cattagttat       60

tggggtttcc caagtgaaga gtaccttgtc gaaacagaag atggctacat tttgtgcttg      120

aataggattc cacatggaag aaagaatcac tccgataagg gcccaaaacc ggttgtattt      180

ctccagcacg gcctgctggc agactctagt aattgggtga ccaacctcgc caatagttct      240

ttgggcttca ttctggccga cgctggtttc gacgtatgga tgggaaatag ccggggaaat      300

acgtggagcc gaaagcataa aacgctgtcc gttagccaag acgagttctg ggcatttagc      360

tatgacgaga tggctaaata tgatcttcca gcctcaatta actttatcct gaataagaca      420

ggacaggagc aagtatacta cgtcggccac agtcaaggga caactatagg ctttattgca      480

ttttcacaga ttcccgaatt ggctaaaagg atcaaaatgt tctttgcatt gggtcccgtg      540

gcatctgtag cgttttgtac cagcccgatg gcgaagctgg ggcgcttgcc cgatcatctg      600

attaaagatt tgtttggtga caaggaattt ctgcctcaat ccgcgttcct taagtggctc      660

gggactcatg tttgcaccca tgtaatactg aaggaactgt gcgggaatct ttgcttcttg      720

ttgtgcgggt ttaatgaaag gaatcttaac atgagccgag tggatgtata cacgacacat      780

agccccgctg ggactagcgt gcaaaatatg ctccattggt cccaagctgt gaaatttcaa      840

aaatttcagg ctttcgactg gggctcttca gctaaaaatt atttccacta taaccagtca      900

tatccaccta cgtacaacgt aaaagacatg cttgttccca cggcagtctg gagtggcggc      960

cacgattggt tggcagacgt gtacgatgtc aatatcttgc ttacccagat tacaaacctc     1020

gtcttccatg agagtatacc ggaatgggag catcttgatt tcatttgggg cttggacgca     1080

ccgtggcgat tgtataataa gatcataaac cttatgcgga aatatcagta a              1131


<210>  16
<211>  1131
<212>  DNA
<213>  artificial

<220>
<223>  hLAL w/o signal peptide (from Opt_2)

<400>  16
ggcaagctga ccgccgtgga tcctgaaacc aacatgaacg tgtccgagat catcagctac       60

tggggcttcc cttctgaaga gtacctggtg gaaacagagg acggctacat cctgtgcctg      120

aatagaatcc cccacggcag aaagaaccac tctgataagg gccctaagcc cgtggtcttt      180

ctgcagcacg gcctgctggc cgacagcagc aactgggtga caaacctcgc taattctagc      240

ctgggcttca tcctggccga tgccggcttc gacgtgtgga tgggcaatag ccgtggaaat      300

acctggtccc ggaagcacaa gaccctgagc gtgagccagg acgagttctg ggccttcagc      360

tatgatgaga tggccaagta cgacctgcct gcttctatca acttcattct gaacaagaca      420

ggccaggagc aggtgtacta cgtgggccac agccagggca ccaccatcgg ctttatcgcc      480

tttagccaga tccctgagct ggccaagcgg atcaagatgt tcttcgccct gggacctgtg      540

gcctctgtgg ccttctgcac aagccctatg gctaagctgg gcagactgcc cgaccacctg      600

atcaaggacc tgttcggcga caaggaattc ctgcctcaga gcgccttcct gaagtggctg      660

ggcacacacg tgtgcaccca tgtgatcctg aaagaactgt gtggcaacct gtgcttcctg      720

ctgtgcggct ttaacgagag aaacctgaac atgagcagag tggacgtgta caccacacat      780

agccccgccg gcaccagcgt gcagaacatg ctgcactggt cccaggccgt gaagttccag      840

aagttccaag cttttgactg gggaagcagc gccaaaaact acttccacta caaccagagc      900

taccccccca cctacaatgt gaaagacatg ctcgtgccta ccgccgtgtg gagcggcggc      960

cacgactggc tggccgacgt gtatgacgtg aacatcctgc tgacacagat aacaaacctg     1020

gtgttccacg agagcatccc agaatgggag cacctggact tcatctgggg cctggacgcc     1080

ccttggcggc tgtacaacaa gatcatcaac ctgatgagaa aataccagta a              1131


<210>  17
<211>  1131
<212>  DNA
<213>  artificial

<220>
<223>  hLAL w/o signal peptide (from Opt_3)

<400>  17
ggcaagctga cagccgtgga tcccgagaca aacatgaacg tgtccgagat catcagctac       60

tggggcttcc ccagcgagga atacctggtg gaaaccgagg acggctacat cctgtgcctg      120

aacagaatcc ctcacggccg gaagaaccac agcgacaagg gacctaagcc tgtggtgttt      180

ctgcagcacg gactgctggc cgacagcagc aattgggtca ccaacctggc caatagcagc      240

ctgggcttca ttctggccga tgccggcttc gatgtgtgga tgggcaacag cagaggcaac      300

acctggtcca gaaagcacaa gaccctgagc gtgtcccagg acgagttctg ggccttcagc      360

tacgacgaga tggccaaata cgatctgccc gccagcatca acttcatcct gaacaagacc      420

ggccaagagc aggtctacta cgtgggccac tctcagggca ccaccatcgg ctttatcgca      480

ttctctcaga tccccgagct ggccaagcgg atcaagatgt tctttgctct gggccccgtg      540

gccagcgtgg ccttctgtac atctcctatg gccaagctgg gcagactgcc cgaccacctg      600

atcaaggatc tgttcggcga caaagagttc ctgcctcaga gcgccttcct gaagtggctg      660

ggaacccatg tgtgcaccca cgtgatcctg aaagagctgt gcggcaacct gtgcttcctg      720

ctgtgtggct tcaacgagcg gaacctgaac atgagcagag tggacgtgta caccacacac      780

agccctgccg gaaccagcgt gcagaacatg ctgcattgga gccaggccgt gaagttccag      840

aagtttcagg ccttcgactg gggcagcagc gccaagaact acttccacta caaccagagc      900

tacccgccta cctacaacgt gaaggacatg ctggtgccca ccgccgtttg gagcggagga      960

catgattggc tggctgacgt gtacgacgtg aacatcctgc tgacccagat caccaatctg     1020

gtgttccacg agagcatccc cgagtgggag cacctggatt tcatctgggg actcgacgcc     1080

ccttggcggc tgtacaacaa gatcatcaac ctgatgcgga agtaccagta a              1131


<210>  18
<211>  66
<212>  DNA
<213>  artificial

<220>
<223>  Sp8 (from Opt_1)

<400>  18
atggcatcac gcttgacgtt gttgacactg cttctcttgc ttctggcggg ggacagggcc       60

tcaagc                                                                  66


<210>  19
<211>  66
<212>  DNA
<213>  artificial

<220>
<223>  Sp8 (from Opt_2)

<400>  19
atggccagca gactgaccct gctgaccctg ctgctgctgc tgcttgccgg agatagagcc       60

tcttct                                                                  66


<210>  20
<211>  66
<212>  DNA
<213>  artificial

<220>
<223>  Sp8 (from Opt_3)

<400>  20
atggccagca gactgaccct gctgacactg cttctgctgc tgctggcagg cgatagagcc       60

tcttct                                                                  66


<210>  21
<211>  1197
<212>  DNA
<213>  artificial

<220>
<223>  hLAL+Sp8 (from Opt_1)

<400>  21
atggcatcac gcttgacgtt gttgacactg cttctcttgc ttctggcggg ggacagggcc       60

tcaagcggga agctcacggc agtagacccg gagaccaata tgaatgttag cgagatcatt      120

agttattggg gtttcccaag tgaagagtac cttgtcgaaa cagaagatgg ctacattttg      180

tgcttgaata ggattccaca tggaagaaag aatcactccg ataagggccc aaaaccggtt      240

gtatttctcc agcacggcct gctggcagac tctagtaatt gggtgaccaa cctcgccaat      300

agttctttgg gcttcattct ggccgacgct ggtttcgacg tatggatggg aaatagccgg      360

ggaaatacgt ggagccgaaa gcataaaacg ctgtccgtta gccaagacga gttctgggca      420

tttagctatg acgagatggc taaatatgat cttccagcct caattaactt tatcctgaat      480

aagacaggac aggagcaagt atactacgtc ggccacagtc aagggacaac tataggcttt      540

attgcatttt cacagattcc cgaattggct aaaaggatca aaatgttctt tgcattgggt      600

cccgtggcat ctgtagcgtt ttgtaccagc ccgatggcga agctggggcg cttgcccgat      660

catctgatta aagatttgtt tggtgacaag gaatttctgc ctcaatccgc gttccttaag      720

tggctcggga ctcatgtttg cacccatgta atactgaagg aactgtgcgg gaatctttgc      780

ttcttgttgt gcgggtttaa tgaaaggaat cttaacatga gccgagtgga tgtatacacg      840

acacatagcc ccgctgggac tagcgtgcaa aatatgctcc attggtccca agctgtgaaa      900

tttcaaaaat ttcaggcttt cgactggggc tcttcagcta aaaattattt ccactataac      960

cagtcatatc cacctacgta caacgtaaaa gacatgcttg ttcccacggc agtctggagt     1020

ggcggccacg attggttggc agacgtgtac gatgtcaata tcttgcttac ccagattaca     1080

aacctcgtct tccatgagag tataccggaa tgggagcatc ttgatttcat ttggggcttg     1140

gacgcaccgt ggcgattgta taataagatc ataaacctta tgcggaaata tcagtaa        1197


<210>  22
<211>  1197
<212>  DNA
<213>  artificial

<220>
<223>  hLAL+Sp8 (from Opt_2)

<400>  22
atggccagca gactgaccct gctgaccctg ctgctgctgc tgcttgccgg agatagagcc       60

tcttctggca agctgaccgc cgtggatcct gaaaccaaca tgaacgtgtc cgagatcatc      120

agctactggg gcttcccttc tgaagagtac ctggtggaaa cagaggacgg ctacatcctg      180

tgcctgaata gaatccccca cggcagaaag aaccactctg ataagggccc taagcccgtg      240

gtctttctgc agcacggcct gctggccgac agcagcaact gggtgacaaa cctcgctaat      300

tctagcctgg gcttcatcct ggccgatgcc ggcttcgacg tgtggatggg caatagccgt      360

ggaaatacct ggtcccggaa gcacaagacc ctgagcgtga gccaggacga gttctgggcc      420

ttcagctatg atgagatggc caagtacgac ctgcctgctt ctatcaactt cattctgaac      480

aagacaggcc aggagcaggt gtactacgtg ggccacagcc agggcaccac catcggcttt      540

atcgccttta gccagatccc tgagctggcc aagcggatca agatgttctt cgccctggga      600

cctgtggcct ctgtggcctt ctgcacaagc cctatggcta agctgggcag actgcccgac      660

cacctgatca aggacctgtt cggcgacaag gaattcctgc ctcagagcgc cttcctgaag      720

tggctgggca cacacgtgtg cacccatgtg atcctgaaag aactgtgtgg caacctgtgc      780

ttcctgctgt gcggctttaa cgagagaaac ctgaacatga gcagagtgga cgtgtacacc      840

acacatagcc ccgccggcac cagcgtgcag aacatgctgc actggtccca ggccgtgaag      900

ttccagaagt tccaagcttt tgactgggga agcagcgcca aaaactactt ccactacaac      960

cagagctacc cccccaccta caatgtgaaa gacatgctcg tgcctaccgc cgtgtggagc     1020

ggcggccacg actggctggc cgacgtgtat gacgtgaaca tcctgctgac acagataaca     1080

aacctggtgt tccacgagag catcccagaa tgggagcacc tggacttcat ctggggcctg     1140

gacgcccctt ggcggctgta caacaagatc atcaacctga tgagaaaata ccagtaa        1197


<210>  23
<211>  1197
<212>  DNA
<213>  artificial

<220>
<223>  hLAL+Sp8 (from Opt_3)

<400>  23
atggccagca gactgaccct gctgacactg cttctgctgc tgctggcagg cgatagagcc       60

tcttctggca agctgacagc cgtggatccc gagacaaaca tgaacgtgtc cgagatcatc      120

agctactggg gcttccccag cgaggaatac ctggtggaaa ccgaggacgg ctacatcctg      180

tgcctgaaca gaatccctca cggccggaag aaccacagcg acaagggacc taagcctgtg      240

gtgtttctgc agcacggact gctggccgac agcagcaatt gggtcaccaa cctggccaat      300

agcagcctgg gcttcattct ggccgatgcc ggcttcgatg tgtggatggg caacagcaga      360

ggcaacacct ggtccagaaa gcacaagacc ctgagcgtgt cccaggacga gttctgggcc      420

ttcagctacg acgagatggc caaatacgat ctgcccgcca gcatcaactt catcctgaac      480

aagaccggcc aagagcaggt ctactacgtg ggccactctc agggcaccac catcggcttt      540

atcgcattct ctcagatccc cgagctggcc aagcggatca agatgttctt tgctctgggc      600

cccgtggcca gcgtggcctt ctgtacatct cctatggcca agctgggcag actgcccgac      660

cacctgatca aggatctgtt cggcgacaaa gagttcctgc ctcagagcgc cttcctgaag      720

tggctgggaa cccatgtgtg cacccacgtg atcctgaaag agctgtgcgg caacctgtgc      780

ttcctgctgt gtggcttcaa cgagcggaac ctgaacatga gcagagtgga cgtgtacacc      840

acacacagcc ctgccggaac cagcgtgcag aacatgctgc attggagcca ggccgtgaag      900

ttccagaagt ttcaggcctt cgactggggc agcagcgcca agaactactt ccactacaac      960

cagagctacc cgcctaccta caacgtgaag gacatgctgg tgcccaccgc cgtttggagc     1020

ggaggacatg attggctggc tgacgtgtac gacgtgaaca tcctgctgac ccagatcacc     1080

aatctggtgt tccacgagag catccccgag tgggagcacc tggatttcat ctggggactc     1140

gacgcccctt ggcggctgta caacaagatc atcaacctga tgcggaagta ccagtaa        1197


<210>  24
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  hLAL (sequence mutated in isoform 2)

<400>  24

Asp Gly Tyr Ile Leu Cys Leu Asn Arg Ile Pro His Gly Arg Lys Asn 
1               5                   10                  15      


His Ser Asp Lys 
            20  


<210>  25
<211>  20
<212>  PRT
<213>  artificial

<220>
<223>  hLAL variant isoform 2

<400>  25

Met Ala Cys Leu Glu Phe Val Pro Phe Asp Val Gln Met Cys Leu Glu 
1               5                   10                  15      


Phe Leu Pro Ser 
            20  


<210>  26
<211>  343
<212>  PRT
<213>  Homo sapiens

<400>  26

Met Ala Cys Leu Glu Phe Val Pro Phe Asp Val Gln Met Cys Leu Glu 
1               5                   10                  15      


Phe Leu Pro Ser Gly Pro Lys Pro Val Val Phe Leu Gln His Gly Leu 
            20                  25                  30          


Leu Ala Asp Ser Ser Asn Trp Val Thr Asn Leu Ala Asn Ser Ser Leu 
        35                  40                  45              


Gly Phe Ile Leu Ala Asp Ala Gly Phe Asp Val Trp Met Gly Asn Ser 
    50                  55                  60                  


Arg Gly Asn Thr Trp Ser Arg Lys His Lys Thr Leu Ser Val Ser Gln 
65                  70                  75                  80  


Asp Glu Phe Trp Ala Phe Ser Tyr Asp Glu Met Ala Lys Tyr Asp Leu 
                85                  90                  95      


Pro Ala Ser Ile Asn Phe Ile Leu Asn Lys Thr Gly Gln Glu Gln Val 
            100                 105                 110         


Tyr Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe 
        115                 120                 125             


Ser Gln Ile Pro Glu Leu Ala Lys Arg Ile Lys Met Phe Phe Ala Leu 
    130                 135                 140                 


Gly Pro Val Ala Ser Val Ala Phe Cys Thr Ser Pro Met Ala Lys Leu 
145                 150                 155                 160 


Gly Arg Leu Pro Asp His Leu Ile Lys Asp Leu Phe Gly Asp Lys Glu 
                165                 170                 175     


Phe Leu Pro Gln Ser Ala Phe Leu Lys Trp Leu Gly Thr His Val Cys 
            180                 185                 190         


Thr His Val Ile Leu Lys Glu Leu Cys Gly Asn Leu Cys Phe Leu Leu 
        195                 200                 205             


Cys Gly Phe Asn Glu Arg Asn Leu Asn Met Ser Arg Val Asp Val Tyr 
    210                 215                 220                 


Thr Thr His Ser Pro Ala Gly Thr Ser Val Gln Asn Met Leu His Trp 
225                 230                 235                 240 


Ser Gln Ala Val Lys Phe Gln Lys Phe Gln Ala Phe Asp Trp Gly Ser 
                245                 250                 255     


Ser Ala Lys Asn Tyr Phe His Tyr Asn Gln Ser Tyr Pro Pro Thr Tyr 
            260                 265                 270         


Asn Val Lys Asp Met Leu Val Pro Thr Ala Val Trp Ser Gly Gly His 
        275                 280                 285             


Asp Trp Leu Ala Asp Val Tyr Asp Val Asn Ile Leu Leu Thr Gln Ile 
    290                 295                 300                 


Thr Asn Leu Val Phe His Glu Ser Ile Pro Glu Trp Glu His Leu Asp 
305                 310                 315                 320 


Phe Ile Trp Gly Leu Asp Ala Pro Trp Arg Leu Tyr Asn Lys Ile Ile 
                325                 330                 335     


Asn Leu Met Arg Lys Tyr Gln 
            340             


<210>  27
<211>  1318
<212>  DNA
<213>  artificial

<220>
<223>  Opti-1 (KOZAK + optimized sequence + HA tag).

<400>  27
caccatggca tcacgcttga cgttgttgac actgcttctc ttgcttctgg cgggggacag       60

ggcctcaagc gggaagctca cggcagtaga cccggagacc aatatgaatg ttagcgagat      120

cattagttat tggggtttcc caagtgaaga gtaccttgtc gaaacagaag atggctacat      180

tttgtgcttg aataggattc cacatggaag aaagaatcac tccgataagg gcccaaaacc      240

ggttgtattt ctccagcacg gcctgctggc agactctagt aattgggtga ccaacctcgc      300

caatagttct ttgggcttca ttctggccga cgctggtttc gacgtatgga tgggaaatag      360

ccggggaaat acgtggagcc gaaagcataa aacgctgtcc gttagccaag acgagttctg      420

ggcatttagc tatgacgaga tggctaaata tgatcttcca gcctcaatta actttatcct      480

gaataagaca ggacaggagc aagtatacta cgtcggccac agtcaaggga caactatagg      540

ctttattgca ttttcacaga ttcccgaatt ggctaaaagg atcaaaatgt tctttgcatt      600

gggtcccgtg gcatctgtag cgttttgtac cagcccgatg gcgaagctgg ggcgcttgcc      660

cgatcatctg attaaagatt tgtttggtga caaggaattt ctgcctcaat ccgcgttcct      720

taagtggctc gggactcatg tttgcaccca tgtaatactg aaggaactgt gcgggaatct      780

ttgcttcttg ttgtgcgggt ttaatgaaag gaatcttaac atgagccgag tggatgtata      840

cacgacacat agccccgctg ggactagcgt gcaaaatatg ctccattggt cccaagctgt      900

gaaatttcaa aaatttcagg ctttcgactg gggctcttca gctaaaaatt atttccacta      960

taaccagtca tatccaccta cgtacaacgt aaaagacatg cttgttccca cggcagtctg     1020

gagtggcggc cacgattggt tggcagacgt gtacgatgtc aatatcttgc ttacccagat     1080

tacaaacctc gtcttccatg agagtatacc ggaatgggag catcttgatt tcatttgggg     1140

cttggacgca ccgtggcgat tgtataataa gatcataaac cttatgcgga aatatcaggg     1200

agggagcggc tatccctatg acgtgcctga ttacgccggc acaggatcct acccctatga     1260

tgtgcctgac tacgctggca gcgccggata cccttatgat gtgcctgatt atgcttaa       1318


<210>  28
<211>  1318
<212>  DNA
<213>  artificial

<220>
<223>  Opti-2 (KOZAK + optimized sequence + HA tag).

<400>  28
caccatggcc agcagactga ccctgctgac cctgctgctg ctgctgcttg ccggagatag       60

agcctcttct ggcaagctga ccgccgtgga tcctgaaacc aacatgaacg tgtccgagat      120

catcagctac tggggcttcc cttctgaaga gtacctggtg gaaacagagg acggctacat      180

cctgtgcctg aatagaatcc cccacggcag aaagaaccac tctgataagg gccctaagcc      240

cgtggtcttt ctgcagcacg gcctgctggc cgacagcagc aactgggtga caaacctcgc      300

taattctagc ctgggcttca tcctggccga tgccggcttc gacgtgtgga tgggcaatag      360

ccgtggaaat acctggtccc ggaagcacaa gaccctgagc gtgagccagg acgagttctg      420

ggccttcagc tatgatgaga tggccaagta cgacctgcct gcttctatca acttcattct      480

gaacaagaca ggccaggagc aggtgtacta cgtgggccac agccagggca ccaccatcgg      540

ctttatcgcc tttagccaga tccctgagct ggccaagcgg atcaagatgt tcttcgccct      600

gggacctgtg gcctctgtgg ccttctgcac aagccctatg gctaagctgg gcagactgcc      660

cgaccacctg atcaaggacc tgttcggcga caaggaattc ctgcctcaga gcgccttcct      720

gaagtggctg ggcacacacg tgtgcaccca tgtgatcctg aaagaactgt gtggcaacct      780

gtgcttcctg ctgtgcggct ttaacgagag aaacctgaac atgagcagag tggacgtgta      840

caccacacat agccccgccg gcaccagcgt gcagaacatg ctgcactggt cccaggccgt      900

gaagttccag aagttccaag cttttgactg gggaagcagc gccaaaaact acttccacta      960

caaccagagc taccccccca cctacaatgt gaaagacatg ctcgtgccta ccgccgtgtg     1020

gagcggcggc cacgactggc tggccgacgt gtatgacgtg aacatcctgc tgacacagat     1080

aacaaacctg gtgttccacg agagcatccc agaatgggag cacctggact tcatctgggg     1140

cctggacgcc ccttggcggc tgtacaacaa gatcatcaac ctgatgagaa aataccaggg     1200

agggagcggc tatccctatg acgtgcctga ttacgccggc acaggatcct acccctatga     1260

tgtgcctgac tacgctggca gcgccggata cccttatgat gtgcctgatt atgcttaa       1318


<210>  29
<211>  1318
<212>  DNA
<213>  artificial

<220>
<223>  Opti3 (KOZAK + optimized sequence + HA tag)

<400>  29
caccatggcc agcagactga ccctgctgac actgcttctg ctgctgctgg caggcgatag       60

agcctcttct ggcaagctga cagccgtgga tcccgagaca aacatgaacg tgtccgagat      120

catcagctac tggggcttcc ccagcgagga atacctggtg gaaaccgagg acggctacat      180

cctgtgcctg aacagaatcc ctcacggccg gaagaaccac agcgacaagg gacctaagcc      240

tgtggtgttt ctgcagcacg gactgctggc cgacagcagc aattgggtca ccaacctggc      300

caatagcagc ctgggcttca ttctggccga tgccggcttc gatgtgtgga tgggcaacag      360

cagaggcaac acctggtcca gaaagcacaa gaccctgagc gtgtcccagg acgagttctg      420

ggccttcagc tacgacgaga tggccaaata cgatctgccc gccagcatca acttcatcct      480

gaacaagacc ggccaagagc aggtctacta cgtgggccac tctcagggca ccaccatcgg      540

ctttatcgca ttctctcaga tccccgagct ggccaagcgg atcaagatgt tctttgctct      600

gggccccgtg gccagcgtgg ccttctgtac atctcctatg gccaagctgg gcagactgcc      660

cgaccacctg atcaaggatc tgttcggcga caaagagttc ctgcctcaga gcgccttcct      720

gaagtggctg ggaacccatg tgtgcaccca cgtgatcctg aaagagctgt gcggcaacct      780

gtgcttcctg ctgtgtggct tcaacgagcg gaacctgaac atgagcagag tggacgtgta      840

caccacacac agccctgccg gaaccagcgt gcagaacatg ctgcattgga gccaggccgt      900

gaagttccag aagtttcagg ccttcgactg gggcagcagc gccaagaact acttccacta      960

caaccagagc tacccgccta cctacaacgt gaaggacatg ctggtgccca ccgccgtttg     1020

gagcggagga catgattggc tggctgacgt gtacgacgtg aacatcctgc tgacccagat     1080

caccaatctg gtgttccacg agagcatccc cgagtgggag cacctggatt tcatctgggg     1140

actcgacgcc ccttggcggc tgtacaacaa gatcatcaac ctgatgcgga agtaccaggg     1200

agggagcggc tatccctatg acgtgcctga ttacgccggc acaggatcct acccctatga     1260

tgtgcctgac tacgctggca gcgccggata cccttatgat gtgcctgatt atgcttaa       1318


<210>  30
<211>  401
<212>  PRT
<213>  artificial

<220>
<223>  SP6+hLAL protein

<400>  30

Met Pro Pro Pro Arg Thr Gly Arg Gly Leu Leu Trp Leu Gly Leu Val 
1               5                   10                  15      


Leu Ser Ser Val Cys Val Ala Leu Gly Gly Lys Leu Thr Ala Val Asp 
            20                  25                  30          


Pro Glu Thr Asn Met Asn Val Ser Glu Ile Ile Ser Tyr Trp Gly Phe 
        35                  40                  45              


Pro Ser Glu Glu Tyr Leu Val Glu Thr Glu Asp Gly Tyr Ile Leu Cys 
    50                  55                  60                  


Leu Asn Arg Ile Pro His Gly Arg Lys Asn His Ser Asp Lys Gly Pro 
65                  70                  75                  80  


Lys Pro Val Val Phe Leu Gln His Gly Leu Leu Ala Asp Ser Ser Asn 
                85                  90                  95      


Trp Val Thr Asn Leu Ala Asn Ser Ser Leu Gly Phe Ile Leu Ala Asp 
            100                 105                 110         


Ala Gly Phe Asp Val Trp Met Gly Asn Ser Arg Gly Asn Thr Trp Ser 
        115                 120                 125             


Arg Lys His Lys Thr Leu Ser Val Ser Gln Asp Glu Phe Trp Ala Phe 
    130                 135                 140                 


Ser Tyr Asp Glu Met Ala Lys Tyr Asp Leu Pro Ala Ser Ile Asn Phe 
145                 150                 155                 160 


Ile Leu Asn Lys Thr Gly Gln Glu Gln Val Tyr Tyr Val Gly His Ser 
                165                 170                 175     


Gln Gly Thr Thr Ile Gly Phe Ile Ala Phe Ser Gln Ile Pro Glu Leu 
            180                 185                 190         


Ala Lys Arg Ile Lys Met Phe Phe Ala Leu Gly Pro Val Ala Ser Val 
        195                 200                 205             


Ala Phe Cys Thr Ser Pro Met Ala Lys Leu Gly Arg Leu Pro Asp His 
    210                 215                 220                 


Leu Ile Lys Asp Leu Phe Gly Asp Lys Glu Phe Leu Pro Gln Ser Ala 
225                 230                 235                 240 


Phe Leu Lys Trp Leu Gly Thr His Val Cys Thr His Val Ile Leu Lys 
                245                 250                 255     


Glu Leu Cys Gly Asn Leu Cys Phe Leu Leu Cys Gly Phe Asn Glu Arg 
            260                 265                 270         


Asn Leu Asn Met Ser Arg Val Asp Val Tyr Thr Thr His Ser Pro Ala 
        275                 280                 285             


Gly Thr Ser Val Gln Asn Met Leu His Trp Ser Gln Ala Val Lys Phe 
    290                 295                 300                 


Gln Lys Phe Gln Ala Phe Asp Trp Gly Ser Ser Ala Lys Asn Tyr Phe 
305                 310                 315                 320 


His Tyr Asn Gln Ser Tyr Pro Pro Thr Tyr Asn Val Lys Asp Met Leu 
                325                 330                 335     


Val Pro Thr Ala Val Trp Ser Gly Gly His Asp Trp Leu Ala Asp Val 
            340                 345                 350         


Tyr Asp Val Asn Ile Leu Leu Thr Gln Ile Thr Asn Leu Val Phe His 
        355                 360                 365             


Glu Ser Ile Pro Glu Trp Glu His Leu Asp Phe Ile Trp Gly Leu Asp 
    370                 375                 380                 


Ala Pro Trp Arg Leu Tyr Asn Lys Ile Ile Asn Leu Met Arg Lys Tyr 
385                 390                 395                 400 


Gln 
    


<210>  31
<211>  394
<212>  PRT
<213>  artificial

<220>
<223>  SP7+hLAL protein

<400>  31

Met Ala Phe Leu Trp Leu Leu Ser Cys Trp Ala Leu Leu Gly Thr Thr 
1               5                   10                  15      


Phe Gly Gly Lys Leu Thr Ala Val Asp Pro Glu Thr Asn Met Asn Val 
            20                  25                  30          


Ser Glu Ile Ile Ser Tyr Trp Gly Phe Pro Ser Glu Glu Tyr Leu Val 
        35                  40                  45              


Glu Thr Glu Asp Gly Tyr Ile Leu Cys Leu Asn Arg Ile Pro His Gly 
    50                  55                  60                  


Arg Lys Asn His Ser Asp Lys Gly Pro Lys Pro Val Val Phe Leu Gln 
65                  70                  75                  80  


His Gly Leu Leu Ala Asp Ser Ser Asn Trp Val Thr Asn Leu Ala Asn 
                85                  90                  95      


Ser Ser Leu Gly Phe Ile Leu Ala Asp Ala Gly Phe Asp Val Trp Met 
            100                 105                 110         


Gly Asn Ser Arg Gly Asn Thr Trp Ser Arg Lys His Lys Thr Leu Ser 
        115                 120                 125             


Val Ser Gln Asp Glu Phe Trp Ala Phe Ser Tyr Asp Glu Met Ala Lys 
    130                 135                 140                 


Tyr Asp Leu Pro Ala Ser Ile Asn Phe Ile Leu Asn Lys Thr Gly Gln 
145                 150                 155                 160 


Glu Gln Val Tyr Tyr Val Gly His Ser Gln Gly Thr Thr Ile Gly Phe 
                165                 170                 175     


Ile Ala Phe Ser Gln Ile Pro Glu Leu Ala Lys Arg Ile Lys Met Phe 
            180                 185                 190         


Phe Ala Leu Gly Pro Val Ala Ser Val Ala Phe Cys Thr Ser Pro Met 
        195                 200                 205             


Ala Lys Leu Gly Arg Leu Pro Asp His Leu Ile Lys Asp Leu Phe Gly 
    210                 215                 220                 


Asp Lys Glu Phe Leu Pro Gln Ser Ala Phe Leu Lys Trp Leu Gly Thr 
225                 230                 235                 240 


His Val Cys Thr His Val Ile Leu Lys Glu Leu Cys Gly Asn Leu Cys 
                245                 250                 255     


Phe Leu Leu Cys Gly Phe Asn Glu Arg Asn Leu Asn Met Ser Arg Val 
            260                 265                 270         


Asp Val Tyr Thr Thr His Ser Pro Ala Gly Thr Ser Val Gln Asn Met 
        275                 280                 285             


Leu His Trp Ser Gln Ala Val Lys Phe Gln Lys Phe Gln Ala Phe Asp 
    290                 295                 300                 


Trp Gly Ser Ser Ala Lys Asn Tyr Phe His Tyr Asn Gln Ser Tyr Pro 
305                 310                 315                 320 


Pro Thr Tyr Asn Val Lys Asp Met Leu Val Pro Thr Ala Val Trp Ser 
                325                 330                 335     


Gly Gly His Asp Trp Leu Ala Asp Val Tyr Asp Val Asn Ile Leu Leu 
            340                 345                 350         


Thr Gln Ile Thr Asn Leu Val Phe His Glu Ser Ile Pro Glu Trp Glu 
        355                 360                 365             


His Leu Asp Phe Ile Trp Gly Leu Asp Ala Pro Trp Arg Leu Tyr Asn 
    370                 375                 380                 


Lys Ile Ile Asn Leu Met Arg Lys Tyr Gln 
385                 390                 


<210>  32
<211>  398
<212>  PRT
<213>  artificial

<220>
<223>  SP8+hLAL protein

<400>  32

Met Ala Ser Arg Leu Thr Leu Leu Thr Leu Leu Leu Leu Leu Leu Ala 
1               5                   10                  15      


Gly Asp Arg Ala Ser Ser Gly Lys Leu Thr Ala Val Asp Pro Glu Thr 
            20                  25                  30          


Asn Met Asn Val Ser Glu Ile Ile Ser Tyr Trp Gly Phe Pro Ser Glu 
        35                  40                  45              


Glu Tyr Leu Val Glu Thr Glu Asp Gly Tyr Ile Leu Cys Leu Asn Arg 
    50                  55                  60                  


Ile Pro His Gly Arg Lys Asn His Ser Asp Lys Gly Pro Lys Pro Val 
65                  70                  75                  80  


Val Phe Leu Gln His Gly Leu Leu Ala Asp Ser Ser Asn Trp Val Thr 
                85                  90                  95      


Asn Leu Ala Asn Ser Ser Leu Gly Phe Ile Leu Ala Asp Ala Gly Phe 
            100                 105                 110         


Asp Val Trp Met Gly Asn Ser Arg Gly Asn Thr Trp Ser Arg Lys His 
        115                 120                 125             


Lys Thr Leu Ser Val Ser Gln Asp Glu Phe Trp Ala Phe Ser Tyr Asp 
    130                 135                 140                 


Glu Met Ala Lys Tyr Asp Leu Pro Ala Ser Ile Asn Phe Ile Leu Asn 
145                 150                 155                 160 


Lys Thr Gly Gln Glu Gln Val Tyr Tyr Val Gly His Ser Gln Gly Thr 
                165                 170                 175     


Thr Ile Gly Phe Ile Ala Phe Ser Gln Ile Pro Glu Leu Ala Lys Arg 
            180                 185                 190         


Ile Lys Met Phe Phe Ala Leu Gly Pro Val Ala Ser Val Ala Phe Cys 
        195                 200                 205             


Thr Ser Pro Met Ala Lys Leu Gly Arg Leu Pro Asp His Leu Ile Lys 
    210                 215                 220                 


Asp Leu Phe Gly Asp Lys Glu Phe Leu Pro Gln Ser Ala Phe Leu Lys 
225                 230                 235                 240 


Trp Leu Gly Thr His Val Cys Thr His Val Ile Leu Lys Glu Leu Cys 
                245                 250                 255     


Gly Asn Leu Cys Phe Leu Leu Cys Gly Phe Asn Glu Arg Asn Leu Asn 
            260                 265                 270         


Met Ser Arg Val Asp Val Tyr Thr Thr His Ser Pro Ala Gly Thr Ser 
        275                 280                 285             


Val Gln Asn Met Leu His Trp Ser Gln Ala Val Lys Phe Gln Lys Phe 
    290                 295                 300                 


Gln Ala Phe Asp Trp Gly Ser Ser Ala Lys Asn Tyr Phe His Tyr Asn 
305                 310                 315                 320 


Gln Ser Tyr Pro Pro Thr Tyr Asn Val Lys Asp Met Leu Val Pro Thr 
                325                 330                 335     


Ala Val Trp Ser Gly Gly His Asp Trp Leu Ala Asp Val Tyr Asp Val 
            340                 345                 350         


Asn Ile Leu Leu Thr Gln Ile Thr Asn Leu Val Phe His Glu Ser Ile 
        355                 360                 365             


Pro Glu Trp Glu His Leu Asp Phe Ile Trp Gly Leu Asp Ala Pro Trp 
    370                 375                 380                 


Arg Leu Tyr Asn Lys Ile Ile Asn Leu Met Arg Lys Tyr Gln 
385                 390                 395             


<210>  33
<211>  1197
<212>  DNA
<213>  artificial

<220>
<223>  coding sequence sp8+LAL

<400>  33
atggcctcca ggctgaccct gctgaccctc ctgctgctgc tgctggctgg ggatagagcc       60

tcctcaggga aactgacagc tgtggatcct gaaacaaaca tgaatgtgag tgaaattatc      120

tcttactggg gattccctag tgaggaatac ctagttgaga cagaagatgg atatattctg      180

tgccttaacc gaattcctca tgggaggaag aaccattctg acaaaggtcc caaaccagtt      240

gtcttcctgc aacatggctt gctggcagat tctagtaact gggtcacaaa ccttgccaac      300

agcagcctgg gcttcattct tgctgatgct ggttttgacg tgtggatggg caacagcaga      360

ggaaatacct ggtctcggaa acataagaca ctctcagttt ctcaggatga attctgggct      420

ttcagttatg atgagatggc aaaatatgac ctaccagctt ccattaactt cattctgaat      480

aaaactggcc aagaacaagt gtattatgtg ggtcattctc aaggcaccac tataggtttt      540

atagcatttt cacagatccc tgagctggct aaaaggatta aaatgttttt tgccctgggt      600

cctgtggctt ccgtcgcctt ctgtactagc cctatggcca aattaggacg attaccagat      660

catctcatta aggacttatt tggagacaaa gaatttcttc cccagagtgc gtttttgaag      720

tggctgggta cccacgtttg cactcatgtc atactgaagg agctctgtgg aaatctctgt      780

tttcttctgt gtggatttaa tgagagaaat ttaaatatgt ctagagtgga tgtatataca      840

acacattctc ctgctggaac ttctgtgcaa aacatgttac actggagcca ggctgttaaa      900

ttccaaaagt ttcaagcctt tgactgggga agcagtgcca agaattattt tcattacaac      960

cagagttatc ctcccacata caatgtgaag gacatgcttg tgccgactgc agtctggagc     1020

gggggtcacg actggcttgc agatgtctac gacgtcaata tcttactgac tcagatcacc     1080

aacttggtgt tccatgagag cattccggaa tgggagcatc ttgacttcat ttggggcctg     1140

gatgcccctt ggaggcttta taataaaatt attaatctaa tgaggaaata tcagtaa        1197


