                         SEQUENCE LISTING

<110>  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY
 
<120>  METHODS AND KITS FOR INDUCING IMMUNOTOLERANCE TO GENE DELIVERING 
       TARGET VEHICLE

<130>  075580-8004WO02

<150>  PCT/CN2020/134283
<151>  2020-12-07

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTLA-4 full length protein sequence with signal peptide

<400>  1

Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala 
1               5                   10                  15      


Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro 
            20                  25                  30          


Val Phe Cys Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala 
        35                  40                  45              


Ser Ser Arg Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly 
    50                  55                  60                  


Lys Ala Thr Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln 
65                  70                  75                  80  


Val Thr Glu Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr 
                85                  90                  95      


Phe Leu Asp Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val 
            100                 105                 110         


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
        115                 120                 125             


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly 
    130                 135                 140                 


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
145                 150                 155                 160 


Asp Phe Leu Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe 
                165                 170                 175     


Tyr Ser Phe Leu Leu Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys 
            180                 185                 190         


Arg Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu 
        195                 200                 205             


Pro Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
    210                 215                 220             


<210>  2
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD28ECD

<400>  2

Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr Asp Asn 
1               5                   10                  15      


Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser Arg Glu 
            20                  25                  30          


Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu Val Cys 
        35                  40                  45              


Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser Lys Thr 
    50                  55                  60                  


Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr Phe Tyr 
65                  70                  75                  80  


Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys Lys Ile 
                85                  90                  95      


Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn Gly 
            100                 105                 110         


Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu Phe 
        115                 120                 125             


Pro Gly Pro Ser Lys Pro 
    130                 


<210>  3
<211>  124
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTLA-4 extracellular domain without signal peptide

<400>  3

Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg Gly Ile 
1               5                   10                  15      


Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr Glu Val 
            20                  25                  30          


Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu Val Cys 
        35                  40                  45              


Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp Asp Ser 
    50                  55                  60                  


Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr Ile Gln 
65                  70                  75                  80  


Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val Glu Leu 
                85                  90                  95      


Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr Gln Ile 
            100                 105                 110         


Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp 
        115                 120                 


<210>  4
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40 extracellular domain without signal peptide

<400>  4

Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu Ile Asn Ser Gln 
1               5                   10                  15      


Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val Ser Asp Cys Thr 
            20                  25                  30          


Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu Ser Glu Phe Leu 
        35                  40                  45              


Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His Lys Tyr Cys Asp 
    50                  55                  60                  


Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr Ser Glu Thr Asp 
65                  70                  75                  80  


Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr Ser Glu Ala Cys 
                85                  90                  95      


Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly Phe Gly Val Lys 
            100                 105                 110         


Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu Pro Cys Pro Val 
        115                 120                 125             


Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys Cys His Pro Trp 
    130                 135                 140                 


Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln Ala Gly Thr Asn 
145                 150                 155                 160 


Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu Arg 
                165                 170             


<210>  5
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human Fc polypeptide sequence

<400>  5

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  6
<211>  35
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTLA-4 signal peptide sequence

<400>  6

Met Ala Cys Leu Gly Phe Gln Arg His Lys Ala Gln Leu Asn Leu Ala 
1               5                   10                  15      


Thr Arg Thr Trp Pro Cys Thr Leu Leu Phe Phe Leu Leu Phe Ile Pro 
            20                  25                  30          


Val Phe Cys 
        35  


<210>  7
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD-40 signal peptide sequence

<400>  7

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro 
            20  


<210>  8
<211>  397
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CTLA4-Ig full sequence with signal peptide

<400>  8

Met Ala Cys Leu Gly Leu Arg Arg Tyr Lys Ala Gln Leu Gln Leu Pro 
1               5                   10                  15      


Ser Arg Thr Trp Pro Phe Val Ala Leu Leu Thr Leu Leu Phe Ile Pro 
            20                  25                  30          


Val Phe Ser Glu Ala Ile Gln Val Thr Gln Pro Ser Val Val Leu Ala 
        35                  40                  45              


Ser Ser His Gly Val Ala Ser Phe Pro Cys Glu Tyr Ser Pro Ser His 
    50                  55                  60                  


Asn Thr Asp Glu Val Arg Val Thr Val Leu Arg Gln Thr Asn Asp Gln 
65                  70                  75                  80  


Met Thr Glu Val Cys Ala Thr Thr Phe Thr Glu Lys Asn Thr Val Gly 
                85                  90                  95      


Phe Leu Asp Tyr Pro Phe Cys Ser Gly Thr Phe Asn Glu Ser Arg Val 
            100                 105                 110         


Asn Leu Thr Ile Gln Gly Leu Arg Ala Val Asp Thr Gly Leu Tyr Leu 
        115                 120                 125             


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Phe Val Gly Met Gly 
    130                 135                 140                 


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
145                 150                 155                 160 


Asp Leu Ile Ser Glu Pro Arg Gly Pro Thr Ile Lys Pro Cys Pro Pro 
                165                 170                 175     


Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln 
    210                 215                 220                 


Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln 
225                 230                 235                 240 


Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu 
                245                 250                 255     


Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys 
        275                 280                 285             


Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro 
    290                 295                 300                 


Gln Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr 
305                 310                 315                 320 


Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys 
                325                 330                 335     


Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly 
            340                 345                 350         


Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val 
        355                 360                 365             


Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn 
    370                 375                 380                 


His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
385                 390                 395         


<210>  9
<211>  422
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40-Ig full sequence with signal peptide

<400>  9

Met Val Ser Leu Pro Arg Leu Cys Ala Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Leu Gly Gln Cys Val Thr Cys Ile Asp Lys Gln Tyr Leu 
            20                  25                  30          


His Asp Gly Gln Cys Cys Asp Leu Cys Gln Pro Gly Ser Arg Leu Thr 
        35                  40                  45              


Ser His Cys Thr Ala Leu Glu Lys Thr Gln Cys His Pro Cys Asp Ser 
    50                  55                  60                  


Gly Glu Phe Ser Ala Gln Trp Asn Arg Glu Ile Arg Cys His Gln His 
65                  70                  75                  80  


Arg His Cys Glu Pro Asn Gln Gly Leu Arg Val Lys Lys Glu Gly Thr 
                85                  90                  95      


Ala Glu Ser Asp Thr Val Cys Thr Cys Lys Glu Gly Gln His Cys Thr 
            100                 105                 110         


Ser Lys Asp Cys Glu Ala Cys Ala Gln His Thr Pro Cys Ile Pro Gly 
        115                 120                 125             


Phe Gly Val Met Glu Met Ala Thr Glu Thr Thr Asp Thr Val Cys His 
    130                 135                 140                 


Pro Cys Pro Val Gly Phe Phe Ser Asn Gln Ser Ser Leu Phe Glu Lys 
145                 150                 155                 160 


Cys Tyr Pro Trp Thr Ser Cys Glu Asp Lys Asn Leu Glu Val Leu Gln 
                165                 170                 175     


Lys Gly Thr Ser Gln Thr Asn Val Ile Cys Gly Leu Lys Ser Arg Met 
            180                 185                 190         


Arg Gly Thr Val Pro Arg Asp Cys Gly Cys Glu Pro Cys Ile Cys Thr 
        195                 200                 205             


Val Pro Glu Val Ser Ser Val Ile Ile Phe Pro Pro Lys Pro Lys Asp 
    210                 215                 220                 


Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr Cys Val Val Val Asp 
225                 230                 235                 240 


Ile Ser Lys Asp Asp Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp 
                245                 250                 255     


Val Glu Val His Thr Ala Gln Thr Gln Pro Arg Glu Glu Gln Leu Asn 
            260                 265                 270         


Ser Ala Phe Arg Ser Val Gly Glu Leu Pro Ile Met His Gln Asp Trp 
        275                 280                 285             


Leu Asn Gly Lys Glu Phe Lys Met Gln Gly Gln Gln Cys Ser Phe Pro 
    290                 295                 300                 


Cys Pro His Arg Glu Asn His Leu Pro Thr Pro Gln Lys Pro Lys Ala 
305                 310                 315                 320 


Pro Gln Val Tyr Thr Ile Pro Pro Pro Lys Glu Gln Met Ala Lys Asp 
                325                 330                 335     


Lys Val Ser Leu Thr Cys Met Ile Thr Asp Phe Phe Pro Glu Asp Ile 
            340                 345                 350         


Thr Val Glu Trp Gln Trp Asn Gly Gln Pro Ala Glu Asn Tyr Lys Asn 
        355                 360                 365             


Thr Gln Pro Ile Met Asp Thr Asp Gly Ser Tyr Phe Val Tyr Ser Lys 
    370                 375                 380                 


Leu Asn Val Gln Lys Ser Asn Trp Glu Ala Gly Asn Thr Phe Thr Cys 
385                 390                 395                 400 


Ser Val Leu His Glu Gly Leu His Asn His His Thr Glu Lys Ser Leu 
                405                 410                 415     


Ser His Ser Pro Gly Lys 
            420         


<210>  10
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Oncostatin M signal peptide

<400>  10

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser Met Ala 
            20                  25      


<210>  11
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTLA-4 extracellular domain without signal peptide

<400>  11

Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg 
1               5                   10                  15      


Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr 
            20                  25                  30          


Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu 
        35                  40                  45              


Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp 
    50                  55                  60                  


Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr 
65                  70                  75                  80  


Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val 
                85                  90                  95      


Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr 
            100                 105                 110         


Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp 
        115                 120                 125     


<210>  12
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CTLA-4 full length protein sequence

<400>  12

Met Ala Cys Leu Gly Leu Arg Arg Tyr Lys Ala Gln Leu Gln Leu Pro 
1               5                   10                  15      


Ser Arg Thr Trp Pro Phe Val Ala Leu Leu Thr Leu Leu Phe Ile Pro 
            20                  25                  30          


Val Phe Ser Glu Ala Ile Gln Val Thr Gln Pro Ser Val Val Leu Ala 
        35                  40                  45              


Ser Ser His Gly Val Ala Ser Phe Pro Cys Glu Tyr Ser Pro Ser His 
    50                  55                  60                  


Asn Thr Asp Glu Val Arg Val Thr Val Leu Arg Gln Thr Asn Asp Gln 
65                  70                  75                  80  


Met Thr Glu Val Cys Ala Thr Thr Phe Thr Glu Lys Asn Thr Val Gly 
                85                  90                  95      


Phe Leu Asp Tyr Pro Phe Cys Ser Gly Thr Phe Asn Glu Ser Arg Val 
            100                 105                 110         


Asn Leu Thr Ile Gln Gly Leu Arg Ala Val Asp Thr Gly Leu Tyr Leu 
        115                 120                 125             


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Phe Val Gly Met Gly 
    130                 135                 140                 


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
145                 150                 155                 160 


Asp Phe Leu Leu Trp Ile Leu Val Ala Val Ser Leu Gly Leu Phe Phe 
                165                 170                 175     


Tyr Ser Phe Leu Val Ser Ala Val Ser Leu Ser Lys Met Leu Lys Lys 
            180                 185                 190         


Arg Ser Pro Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu 
        195                 200                 205             


Pro Glu Cys Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
    210                 215                 220             


<210>  13
<211>  126
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CTLA-4 extracellular domain

<400>  13

Glu Ala Ile Gln Val Thr Gln Pro Ser Val Val Leu Ala Ser Ser His 
1               5                   10                  15      


Gly Val Ala Ser Phe Pro Cys Glu Tyr Ser Pro Ser His Asn Thr Asp 
            20                  25                  30          


Glu Val Arg Val Thr Val Leu Arg Gln Thr Asn Asp Gln Met Thr Glu 
        35                  40                  45              


Val Cys Ala Thr Thr Phe Thr Glu Lys Asn Thr Val Gly Phe Leu Asp 
    50                  55                  60                  


Tyr Pro Phe Cys Ser Gly Thr Phe Asn Glu Ser Arg Val Asn Leu Thr 
65                  70                  75                  80  


Ile Gln Gly Leu Arg Ala Val Asp Thr Gly Leu Tyr Leu Cys Lys Val 
                85                  90                  95      


Glu Leu Met Tyr Pro Pro Pro Tyr Phe Val Gly Met Gly Asn Gly Thr 
            100                 105                 110         


Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp 
        115                 120                 125     


<210>  14
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40 extracellular domain

<400>  14

Leu Gly Gln Cys Val Thr Cys Ser Asp Lys Gln Tyr Leu His Asp Gly 
1               5                   10                  15      


Gln Cys Cys Asp Leu Cys Gln Pro Gly Ser Arg Leu Thr Ser His Cys 
            20                  25                  30          


Thr Ala Leu Glu Lys Thr Gln Cys His Pro Cys Asp Ser Gly Glu Phe 
        35                  40                  45              


Ser Ala Gln Trp Asn Arg Glu Ile Arg Cys His Gln His Arg His Cys 
    50                  55                  60                  


Glu Pro Asn Gln Gly Leu Arg Val Lys Lys Glu Gly Thr Ala Glu Ser 
65                  70                  75                  80  


Asp Thr Val Cys Thr Cys Lys Glu Gly Gln His Cys Thr Ser Lys Asp 
                85                  90                  95      


Cys Glu Ala Cys Ala Gln His Thr Pro Cys Ile Pro Gly Phe Gly Val 
            100                 105                 110         


Met Glu Met Ala Thr Glu Thr Thr Asp Thr Val Cys His Pro Cys Pro 
        115                 120                 125             


Val Gly Phe Phe Ser Asn Gln Ser Ser Leu Phe Glu Lys Cys Tyr Pro 
    130                 135                 140                 


Trp Thr Ser Cys Glu Asp Lys Asn Leu Glu Val Leu Gln Lys Gly Thr 
145                 150                 155                 160 


Ser Gln Thr Asn Val Ile Cys Gly Leu Lys Ser Arg Met Arg 
                165                 170                 


<210>  15
<211>  188
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CTLA-4 full length protein sequence without signal peptide

<400>  15

Lys Ala Met His Val Ala Gln Pro Ala Val Val Leu Ala Ser Ser Arg 
1               5                   10                  15      


Gly Ile Ala Ser Phe Val Cys Glu Tyr Ala Ser Pro Gly Lys Ala Thr 
            20                  25                  30          


Glu Val Arg Val Thr Val Leu Arg Gln Ala Asp Ser Gln Val Thr Glu 
        35                  40                  45              


Val Cys Ala Ala Thr Tyr Met Met Gly Asn Glu Leu Thr Phe Leu Asp 
    50                  55                  60                  


Asp Ser Ile Cys Thr Gly Thr Ser Ser Gly Asn Gln Val Asn Leu Thr 
65                  70                  75                  80  


Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile Cys Lys Val 
                85                  90                  95      


Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Leu Gly Ile Gly Asn Gly Thr 
            100                 105                 110         


Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser Asp Phe Leu 
        115                 120                 125             


Leu Trp Ile Leu Ala Ala Val Ser Ser Gly Leu Phe Phe Tyr Ser Phe 
    130                 135                 140                 


Leu Leu Thr Ala Val Ser Leu Ser Lys Met Leu Lys Lys Arg Ser Pro 
145                 150                 155                 160 


Leu Thr Thr Gly Val Tyr Val Lys Met Pro Pro Thr Glu Pro Glu Cys 
                165                 170                 175     


Glu Lys Gln Phe Gln Pro Tyr Phe Ile Pro Ile Asn 
            180                 185             


<210>  16
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40 full length protein sequence with signal peptide

<400>  16

Met Val Arg Leu Pro Leu Gln Cys Val Leu Trp Gly Cys Leu Leu Thr 
1               5                   10                  15      


Ala Val His Pro Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu 
            20                  25                  30          


Ile Asn Ser Gln Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val 
        35                  40                  45              


Ser Asp Cys Thr Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu 
    50                  55                  60                  


Ser Glu Phe Leu Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His 
65                  70                  75                  80  


Lys Tyr Cys Asp Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr 
                85                  90                  95      


Ser Glu Thr Asp Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr 
            100                 105                 110         


Ser Glu Ala Cys Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly 
        115                 120                 125             


Phe Gly Val Lys Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu 
    130                 135                 140                 


Pro Cys Pro Val Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys 
145                 150                 155                 160 


Cys His Pro Trp Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln 
                165                 170                 175     


Ala Gly Thr Asn Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu 
            180                 185                 190         


Arg Ala Leu Val Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile 
        195                 200                 205             


Leu Leu Val Leu Val Phe Ile Lys Lys Val Ala Lys Lys Pro Thr Asn 
    210                 215                 220                 


Lys Ala Pro His Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp 
225                 230                 235                 240 


Asp Leu Pro Gly Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His 
                245                 250                 255     


Gly Cys Gln Pro Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser 
            260                 265                 270         


Val Gln Glu Arg Gln 
        275         


<210>  17
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40 full length protein sequence with signal peptide

<400>  17

Glu Pro Pro Thr Ala Cys Arg Glu Lys Gln Tyr Leu Ile Asn Ser Gln 
1               5                   10                  15      


Cys Cys Ser Leu Cys Gln Pro Gly Gln Lys Leu Val Ser Asp Cys Thr 
            20                  25                  30          


Glu Phe Thr Glu Thr Glu Cys Leu Pro Cys Gly Glu Ser Glu Phe Leu 
        35                  40                  45              


Asp Thr Trp Asn Arg Glu Thr His Cys His Gln His Lys Tyr Cys Asp 
    50                  55                  60                  


Pro Asn Leu Gly Leu Arg Val Gln Gln Lys Gly Thr Ser Glu Thr Asp 
65                  70                  75                  80  


Thr Ile Cys Thr Cys Glu Glu Gly Trp His Cys Thr Ser Glu Ala Cys 
                85                  90                  95      


Glu Ser Cys Val Leu His Arg Ser Cys Ser Pro Gly Phe Gly Val Lys 
            100                 105                 110         


Gln Ile Ala Thr Gly Val Ser Asp Thr Ile Cys Glu Pro Cys Pro Val 
        115                 120                 125             


Gly Phe Phe Ser Asn Val Ser Ser Ala Phe Glu Lys Cys His Pro Trp 
    130                 135                 140                 


Thr Ser Cys Glu Thr Lys Asp Leu Val Val Gln Gln Ala Gly Thr Asn 
145                 150                 155                 160 


Lys Thr Asp Val Val Cys Gly Pro Gln Asp Arg Leu Arg Ala Leu Val 
                165                 170                 175     


Val Ile Pro Ile Ile Phe Gly Ile Leu Phe Ala Ile Leu Leu Val Leu 
            180                 185                 190         


Val Phe Ile Lys Lys Val Ala Lys Lys Pro Thr Asn Lys Ala Pro His 
        195                 200                 205             


Pro Lys Gln Glu Pro Gln Glu Ile Asn Phe Pro Asp Asp Leu Pro Gly 
    210                 215                 220                 


Ser Asn Thr Ala Ala Pro Val Gln Glu Thr Leu His Gly Cys Gln Pro 
225                 230                 235                 240 


Val Thr Gln Glu Asp Gly Lys Glu Ser Arg Ile Ser Val Gln Glu Arg 
                245                 250                 255     


Gln 
    


