                               SEQUENCE LISTING

<110> THE JOHNS HOPKINS UNIVERSITY
      FUNDACION INSTITUTO LELOIR
      CONSEJO NACIONAL DE INVESTIGACION CIENTIFICA Y 
      TECNICA (CONICET)
      GABELLI, SANDRA B.
      AMZEL, LEON M.
      SRINIVASAN, LAKSHMI
      ALZOGARAY, VANINA
      BEN-JOHNY, MANU
 
<120> NANOBODIES WITH SPECIFIC AFFINITY FOR VOLTAGE GATED SODIUM 
      CHANNELS

<130> JHU4310-1WO

<140> PCT/US2021/061874
<141> 2021-12-03

<150> 63/121,078
<151> 2020-12-03

<160> 43    

<170> PatentIn version 3.5

<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 1
gtcctggctg ctcttctaca agg                                               23


<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 2
ggtacgtgct gttgaactgt tcc                                               23


<210> 3
<211> 29
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 3
gatgtgcagc tgcaggagtc tggrggagg                                         29


<210> 4
<211> 35
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 4
ggactagtgc ggccgctgga gacggtgacc tgggt                                  35


<210> 5
<211> 144
<212> PRT
<213> Lama glama

<400> 5
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Arg Ala Ser Gly Phe Thr Phe Ala Asn Tyr 
            20                  25                  30          


Asp Leu Ala Trp Thr Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Ala Arg Gly Arg Thr Arg Thr Pro Ala Thr Phe 
    50                  55                  60                  


Tyr Ser Ala Ser Ala Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Ala 
65                  70                  75                  80  


Arg Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser 
                85                  90                  95      


Ala Val Tyr Tyr Cys Ala Ala Lys Asp Val Glu Val Ser Val Ala Thr 
            100                 105                 110         


Val Glu Asp Tyr Pro Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135                 140                 


<210> 6
<211> 139
<212> PRT
<213> Lama glama

<400> 6
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ala Asn Tyr 
            20                  25                  30          


Asp Leu Ala Trp Ser Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Thr Arg Asn Pro Pro Tyr Tyr Ser Gly Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Glu Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro 
            100                 105                 110         


Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120                 125             


Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135                 


<210> 7
<211> 136
<212> PRT
<213> Lama glama

<400> 7
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 8
<211> 144
<212> PRT
<213> Lama glama

<400> 8
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Arg Ala Ser Gly Phe Thr Phe Ala Asn Tyr 
            20                  25                  30          


Asp Leu Ala Trp Thr Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Ala Arg Gly Arg Thr Arg Thr Pro Ala Thr Phe 
    50                  55                  60                  


Tyr Ser Ala Ser Val Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Ala 
65                  70                  75                  80  


Arg Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser 
                85                  90                  95      


Ala Val Tyr Tyr Cys Ala Ala Lys Asp Val Glu Val Ser Val Ala Thr 
            100                 105                 110         


Val Glu Asp Tyr Pro Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135                 140                 


<210> 9
<211> 134
<212> PRT
<213> Lama glama

<400> 9
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Thr 
1               5                   10                  15      


Ser Leu Val Leu Ser Cys Ala Ile Ser Gly His Thr Phe Ser Ile Thr 
            20                  25                  30          


Gly Thr Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Leu Thr Ser Ser Gly Asp Ile Thr His Tyr Ala Ser Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Ile Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Ser Val Ile Arg Gly Arg Ala Gly Tyr Asp Thr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Gly Ser Gly Arg Ala 
    130                 


<210> 10
<211> 136
<212> PRT
<213> Lama glama

<400> 10
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 11
<211> 136
<212> PRT
<213> Lama glama

<400> 11
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 12
<211> 136
<212> PRT
<213> Lama glama

<400> 12
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 13
<211> 134
<212> PRT
<213> Lama glama

<400> 13
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Thr 
1               5                   10                  15      


Ser Leu Val Leu Ser Cys Ala Ile Ser Gly His Thr Phe Ser Ile Thr 
            20                  25                  30          


Gly Thr Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Leu Thr Ser Ser Gly Asp Ile Thr His Tyr Ala Ser Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Ile Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Ser Val Ile Arg Gly Arg Ala Gly Tyr Asp Thr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val Pro Asp 
        115                 120                 125             


Tyr Gly Ser Gly Arg Ala 
    130                 


<210> 14
<211> 136
<212> PRT
<213> Lama glama

<400> 14
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 15
<211> 136
<212> PRT
<213> Lama glama

<400> 15
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Ser Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 16
<211> 139
<212> PRT
<213> Lama glama

<400> 16
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Gln Arg Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ala Asn Tyr 
            20                  25                  30          


Asp Leu Ala Trp Ser Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Thr Arg Asn Pro Pro Tyr Tyr Ser Gly Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Glu Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro 
            100                 105                 110         


Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120                 125             


Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135                 


<210> 17
<211> 139
<212> PRT
<213> Lama glama

<400> 17
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Arg Ala Phe Ala Arg Tyr 
            20                  25                  30          


Asp Leu Ala Trp Ser Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Thr Arg Asn Pro Pro Tyr Tyr Ser Gly Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Glu Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro 
            100                 105                 110         


Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120                 125             


Tyr Asp Val Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135                 


<210> 18
<211> 136
<212> PRT
<213> Lama glama

<400> 18
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Ser Gly Arg Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ala Trp Phe Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Arg Ile Ser Arg Ser Gly Gly Ser Thr Met Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Asp Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Phe 
65                  70                  75                  80  


Leu Gln Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro Tyr Asp Val 
        115                 120                 125             


Pro Asp Tyr Gly Ser Gly Arg Ala 
    130                 135     


<210> 19
<211> 9
<212> PRT
<213> Lama glama

<400> 19
Phe Thr Phe Ala Asn Tyr Asp Leu Ala 
1               5                   


<210> 20
<211> 13
<212> PRT
<213> Lama glama

<400> 20
Ser Ile Gly Val Ala Arg Gly Arg Thr Arg Thr Pro Ala 
1               5                   10              


<210> 21
<211> 15
<212> PRT
<213> Lama glama

<400> 21
Lys Asp Val Glu Val Ser Val Ala Thr Val Glu Asp Tyr Pro Tyr 
1               5                   10                  15  


<210> 22
<211> 9
<212> PRT
<213> Lama glama

<400> 22
Leu Thr Phe Ala Asn Tyr Asp Leu Ala 
1               5                   


<210> 23
<211> 9
<212> PRT
<213> Lama glama

<400> 23
Arg Ala Phe Ala Arg Tyr Asp Leu Ala 
1               5                   


<210> 24
<211> 8
<212> PRT
<213> Lama glama

<400> 24
Ser Ile Gly Val Thr Arg Asn Pro 
1               5               


<210> 25
<211> 15
<212> PRT
<213> Lama glama

<400> 25
Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro Tyr 
1               5                   10                  15  


<210> 26
<211> 9
<212> PRT
<213> Lama glama

<400> 26
His Thr Phe Ser Ile Thr Gly Thr Ala 
1               5                   


<210> 27
<211> 8
<212> PRT
<213> Lama glama

<400> 27
Gly Leu Thr Ser Ser Gly Asp Ile 
1               5               


<210> 28
<211> 10
<212> PRT
<213> Lama glama

<400> 28
Val Ile Arg Gly Arg Ala Gly Tyr Asp Thr 
1               5                   10  


<210> 29
<211> 9
<212> PRT
<213> Lama glama

<400> 29
Arg Thr Phe Ser Ser Tyr Ala Met Ala 
1               5                   


<210> 30
<211> 8
<212> PRT
<213> Lama glama

<400> 30
Arg Ile Ser Arg Ser Gly Gly Ser 
1               5               


<210> 31
<211> 12
<212> PRT
<213> Lama glama

<400> 31
Lys Ala Ala Val Ile Leu Thr Gly Ile Pro Asp Tyr 
1               5                   10          


<210> 32
<211> 128
<212> PRT
<213> Lama glama

<400> 32
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Arg Ala Phe Ala Arg Tyr 
            20                  25                  30          


Asp Leu Ala Trp Ser Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Thr Arg Asn Pro Pro Tyr Tyr Ser Gly Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Glu Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro 
            100                 105                 110         


Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120                 125             


<210> 33
<211> 123
<212> PRT
<213> Lama glama

<400> 33
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Thr 
1               5                   10                  15      


Ser Leu Val Leu Ser Cys Ala Ile Ser Gly His Thr Phe Ser Ile Thr 
            20                  25                  30          


Gly Thr Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Gly Leu Thr Ser Ser Gly Asp Ile Thr His Tyr Ala Ser Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Ser Asp Ile Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Thr Ser Val Ile Arg Gly Arg Ala Gly Tyr Asp Thr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120             


<210> 34
<211> 133
<212> PRT
<213> Lama glama

<400> 34
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Arg Ala Ser Gly Phe Thr Phe Ala Asn Tyr 
            20                  25                  30          


Asp Leu Ala Trp Thr Arg Gln Val Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Ala Arg Gly Arg Thr Arg Thr Pro Ala Thr Phe 
    50                  55                  60                  


Tyr Ser Ala Ser Ala Lys Gly Arg Phe Thr Val Ser Arg Asp Asp Ala 
65                  70                  75                  80  


Arg Asn Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Ser 
                85                  90                  95      


Ala Val Tyr Tyr Cys Ala Ala Lys Asp Val Glu Val Ser Val Ala Thr 
            100                 105                 110         


Val Glu Asp Tyr Pro Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser 
        115                 120                 125             


Ser Gly Arg Tyr Pro 
    130             


<210> 35
<211> 128
<212> PRT
<213> Lama glama

<400> 35
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Thr Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Lys Ala Ser Gly Arg Ala Phe Ala Arg Tyr 
            20                  25                  30          


Asp Leu Ala Trp Ser Arg Gln Ala Pro Gly Lys Gln Arg Glu Phe Val 
        35                  40                  45              


Ala Ser Ile Gly Val Thr Arg Asn Pro Pro Tyr Tyr Ser Gly Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Val Ser Arg Asp Asn Ala Lys Glu Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asp Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Lys Asp Ala Ser Val Thr Val Ala Thr Ile Glu Asp Tyr Pro 
            100                 105                 110         


Tyr Trp Gly Arg Gly Thr Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120                 125             


<210> 36
<211> 127
<212> PRT
<213> Lama glama

<400> 36
Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Arg Thr Phe Ser Asp Tyr 
            20                  25                  30          


Asp Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Asp Arg Val 
        35                  40                  45              


Ser Ala Ile Ser Thr Lys Gly Gly Ser Thr Trp Tyr His Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Gly Ala Val Thr Tyr Tyr Ser Ala Arg Tyr Glu Tyr Asp Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125         


<210> 37
<211> 122
<212> PRT
<213> Lama glama

<400> 37
Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Arg Thr Phe Ser Asn Tyr 
            20                  25                  30          


Tyr Ser Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Leu 
        35                  40                  45              


Ala Ser Ile Arg Trp Ser Asp Ser Thr Ile Asn Tyr Ala Ser Asp Ser 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Thr Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Gln Met Asn Ser Leu Lys Leu Glu Asp Thr Ala Val Tyr His 
                85                  90                  95      


Cys Ala Ala Arg Arg Leu Ala Thr Tyr Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Gln Val Thr Val Ser Ser Gly Arg Tyr Pro 
        115                 120         


<210> 38
<211> 128
<212> PRT
<213> Lama glama

<400> 38
Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly Ser Leu 
1               5                   10                  15      


Arg Val Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Tyr Ser Met 
            20                  25                  30          


Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val Ala Ala 
        35                  40                  45              


Ile Ser Arg Ser Asp Asn Ser Thr Tyr Tyr Ala Asp Ser Val Lys Gly 
    50                  55                  60                  


Arg Phe Thr Ile Ser Arg Asp Ser Ala Lys Asn Thr Val Tyr Leu Gln 
65                  70                  75                  80  


Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala Ala 
                85                  90                  95      


Thr Pro Tyr Gly Ser Arg Tyr Tyr Leu Arg Glu Leu Arg Glu Tyr Asp 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125             


<210> 39
<211> 238
<212> PRT
<213> Homo sapiens

<400> 39
Glu Asn Phe Asn Val Ala Thr Glu Glu Ser Ser Glu Pro Leu Gly Glu 
1               5                   10                  15      


Asp Asp Phe Glu Met Phe Tyr Glu Thr Trp Glu Lys Phe Asp Pro Asp 
            20                  25                  30          


Ala Thr Gln Phe Ile Ala Tyr Ser Arg Leu Ser Asp Phe Val Asp Thr 
        35                  40                  45              


Leu Gln Glu Pro Leu Arg Ile Ala Lys Pro Asn Lys Ile Lys Leu Ile 
    50                  55                  60                  


Thr Leu Asp Leu Pro Met Val Pro Gly Asp Lys Ile His Cys Leu Asp 
65                  70                  75                  80  


Ile Leu Phe Ala Leu Thr Lys Glu Val Leu Gly Asp Ser Gly Glu Met 
                85                  90                  95      


Asp Ala Leu Lys Gln Thr Met Glu Glu Lys Phe Met Ala Ala Asn Pro 
            100                 105                 110         


Ser Lys Val Ser Tyr Glu Pro Ile Thr Thr Thr Leu Lys Arg Lys His 
        115                 120                 125             


Glu Glu Val Cys Ala Ile Lys Ile Gln Arg Ala Tyr Arg Arg His Leu 
    130                 135                 140                 


Leu Gln Arg Ser Met Lys Gln Ala Ser Tyr Met Tyr Arg His Ser His 
145                 150                 155                 160 


Asp Gly Ser Gly Asp Asp Ala Pro Glu Lys Glu Gly Leu Leu Ala Asn 
                165                 170                 175     


Thr Met Ser Lys Met Tyr Gly His Glu Asn Gly Asn Ser Ser Ser Pro 
            180                 185                 190         


Ser Pro Glu Glu Lys Gly Glu Ala Gly Asp Ala Gly Pro Thr Met Gly 
        195                 200                 205             


Leu Met Pro Ile Ser Pro Ser Asp Thr Ala Trp Pro Pro Ala Pro Pro 
    210                 215                 220                 


Pro Gly Gln Thr Val Arg Pro Gly Val Lys Glu Ser Leu Val 
225                 230                 235             


<210> 40
<211> 244
<212> PRT
<213> Homo sapiens

<400> 40
Glu Asn Phe Ser Val Ala Thr Glu Glu Ser Thr Glu Pro Leu Ser Glu 
1               5                   10                  15      


Asp Asp Phe Asp Met Phe Tyr Glu Ile Trp Glu Lys Phe Asp Pro Glu 
            20                  25                  30          


Ala Thr Gln Phe Ile Glu Tyr Ser Val Leu Ser Asp Phe Ala Asp Ala 
        35                  40                  45              


Leu Ser Glu Pro Leu Arg Ile Ala Lys Pro Asn Gln Ile Ser Leu Ile 
    50                  55                  60                  


Asn Met Asp Leu Pro Met Val Ser Gly Asp Arg Ile His Cys Met Asp 
65                  70                  75                  80  


Ile Leu Phe Ala Phe Thr Lys Arg Val Leu Gly Glu Ser Gly Glu Met 
                85                  90                  95      


Asp Ala Leu Lys Ile Gln Met Glu Glu Lys Phe Met Ala Ala Asn Pro 
            100                 105                 110         


Ser Lys Ile Ser Tyr Glu Pro Ile Thr Thr Thr Leu Arg Arg Lys His 
        115                 120                 125             


Glu Glu Val Ser Ala Met Val Ile Gln Arg Ala Phe Arg Arg His Leu 
    130                 135                 140                 


Leu Gln Arg Ser Leu Lys His Ala Ser Phe Leu Phe Arg Gln Gln Ala 
145                 150                 155                 160 


Gly Ser Gly Leu Ser Glu Glu Asp Ala Pro Glu Arg Glu Gly Leu Ile 
                165                 170                 175     


Ala Tyr Val Met Ser Glu Asn Phe Ser Arg Pro Leu Gly Pro Pro Ser 
            180                 185                 190         


Ser Ser Ser Ile Ser Ser Thr Ser Phe Pro Pro Ser Tyr Asp Ser Val 
        195                 200                 205             


Thr Arg Ala Thr Ser Asp Asn Leu Gln Val Arg Gly Ser Asp Tyr Ser 
    210                 215                 220                 


His Ser Glu Asp Leu Ala Asp Phe Pro Pro Ser Pro Asp Arg Asp Arg 
225                 230                 235                 240 


Glu Ser Ile Val 
                


<210> 41
<211> 228
<212> PRT
<213> Homo sapiens

<400> 41
Glu Asn Phe Ser Val Ala Thr Glu Glu Ser Thr Glu Pro Leu Ser Glu 
1               5                   10                  15      


Asp Asp Phe Glu Met Phe Tyr Glu Val Trp Glu Lys Phe Asp Pro Asp 
            20                  25                  30          


Ala Thr Gln Phe Ile Glu Phe Ser Lys Leu Ser Asp Phe Ala Ala Ala 
        35                  40                  45              


Leu Asp Pro Pro Leu Leu Ile Ala Lys Pro Asn Lys Val Gln Leu Ile 
    50                  55                  60                  


Ala Met Asp Leu Pro Met Val Ser Gly Asp Arg Ile His Cys Leu Asp 
65                  70                  75                  80  


Ile Leu Phe Ala Phe Thr Lys Arg Val Leu Gly Glu Ser Gly Glu Met 
                85                  90                  95      


Asp Ser Leu Arg Ser Gln Met Glu Glu Arg Phe Met Ser Ala Asn Pro 
            100                 105                 110         


Ser Lys Val Ser Tyr Glu Pro Ile Thr Thr Thr Leu Lys Arg Lys Gln 
        115                 120                 125             


Glu Asp Val Ser Ala Thr Val Ile Gln Arg Ala Tyr Arg Arg Tyr Arg 
    130                 135                 140                 


Leu Arg Gln Asn Val Lys Asn Ile Ser Ser Ile Tyr Ile Lys Asp Gly 
145                 150                 155                 160 


Asp Arg Asp Asp Asp Leu Leu Asn Lys Lys Asp Met Ala Phe Asp Asn 
                165                 170                 175     


Val Asn Glu Asn Ser Ser Pro Glu Lys Thr Asp Ala Thr Ser Ser Thr 
            180                 185                 190         


Thr Ser Pro Pro Ser Tyr Asp Ser Val Thr Lys Pro Asp Lys Glu Lys 
        195                 200                 205             


Tyr Glu Gln Asp Arg Thr Glu Lys Glu Asp Lys Gly Lys Asp Ser Lys 
    210                 215                 220                 


Glu Ser Lys Lys 
225             


<210> 42
<211> 186
<212> PRT
<213> Homo sapiens

<400> 42
Glu Asn Phe Asn Thr Ala Thr Glu Glu Ser Glu Asp Pro Leu Gly Glu 
1               5                   10                  15      


Asp Asp Phe Asp Ile Phe Tyr Glu Val Trp Glu Lys Phe Asp Pro Glu 
            20                  25                  30          


Ala Thr Gln Phe Ile Lys Tyr Ser Ala Leu Ser Asp Phe Ala Asp Ala 
        35                  40                  45              


Leu Pro Glu Pro Leu Arg Val Ala Lys Pro Asn Lys Tyr Gln Phe Leu 
    50                  55                  60                  


Val Met Asp Leu Pro Met Val Ser Glu Asp Arg Leu His Cys Met Asp 
65                  70                  75                  80  


Ile Leu Phe Ala Phe Thr Ala Arg Val Leu Gly Gly Ser Asp Gly Leu 
                85                  90                  95      


Asp Ser Met Lys Ala Met Met Glu Glu Lys Phe Met Glu Ala Asn Pro 
            100                 105                 110         


Leu Lys Lys Leu Tyr Glu Pro Ile Val Thr Thr Thr Lys Arg Lys Glu 
        115                 120                 125             


Glu Glu Arg Gly Ala Ile Ile Gln Lys Ala Phe Arg Lys Tyr Met Met 
    130                 135                 140                 


Lys Val Thr Lys Gly Asp Gln Gly Asp Gln Asn Asp Leu Glu Asn Gly 
145                 150                 155                 160 


Pro His Ser Pro Leu Gln Thr Leu Cys Asn Gly Asp Leu Ser Ser Phe 
                165                 170                 175     


Gly Val Ala Lys Gly Lys Val His Cys Asp 
            180                 185     


<210> 43
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 43
His His His His His His 
1               5       


