                         SEQUENCE LISTING

<110>  Thomas Jefferson University
       Snook, Adam Eugene
       Mahoney, My Georgia
       Carlson, Robert Devlin
 
<120>  Desmoglein 2-directed chimeric antigen receptor (CAR) constructs 
       and methods of use

<130>  205961-0037-00WO

<150>  US 63/120,356
<151>  2020-12-02

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR1

<400>  1
ggctacacgt tcaccaacta cggt                                              24


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR1

<400>  2

Gly Tyr Thr Phe Thr Asn Tyr Gly 
1               5               


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR2

<400>  3
atcaatactt acaccggtaa tcca                                              24


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR2

<400>  4

Ile Asn Thr Tyr Thr Gly Asn Pro 
1               5               


<210>  5
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR3

<400>  5
gctcgcgaca ggggcaactc cttcgactat                                        30


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC CDR3

<400>  6

Ala Arg Asp Arg Gly Asn Ser Phe Asp Tyr 
1               5                   10  


<210>  7
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC

<400>  7
cagatccagc ttgtgcagag cggccccgag ctgaagaagc ccggggagac tgtcaagatc       60

tcttgcaagg cgtccggcta cacgttcacc aactacggta tgaactgggt gaagcaggcc      120

ccggggcgtg gcttgaaatg gatgggttgg atcaatactt acaccggtaa tccaacctac      180

gcggatgact tcaagggccg cttcgatttt tcgctggaga cctccgctag cactgcctac      240

ctgcaaatta acaacctcaa aaacgaggac atggccatct atttctgtgc tcgcgacagg      300

ggcaactcct tcgactattg gggccagggt accacactga ccgtctcttc t               351


<210>  8
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody HC

<400>  8

Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu Lys Lys Pro Gly Glu 
1               5                   10                  15      


Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asn Tyr 
            20                  25                  30          


Gly Met Asn Trp Val Lys Gln Ala Pro Gly Arg Gly Leu Lys Trp Met 
        35                  40                  45              


Gly Trp Ile Asn Thr Tyr Thr Gly Asn Pro Thr Tyr Ala Asp Asp Phe 
    50                  55                  60                  


Lys Gly Arg Phe Asp Phe Ser Leu Glu Thr Ser Ala Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Met Ala Ile Tyr Phe Cys 
                85                  90                  95      


Ala Arg Asp Arg Gly Asn Ser Phe Asp Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser 
        115         


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR1

<400>  9
gagaacatct actcgaac                                                     18


<210>  10
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR1

<400>  10

Glu Asn Ile Tyr Ser Asn 
1               5       


<210>  11
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR2

<400>  11
atcgccatt                                                                9


<210>  12
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR2

<400>  12

Ile Ala Ile 
1           


<210>  13
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR3

<400>  13
cagcactttt ggggcactcc gcgcacc                                           27


<210>  14
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC CDR3

<400>  14

Gln His Phe Trp Gly Thr Pro Arg Thr 
1               5                   


<210>  15
<211>  321
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC

<400>  15
gacatccaga tgacccagag ccctgctagt ctctccgtgt ccgttggcga gacggtgacc       60

atcacctgcc gcgcatccga gaacatctac tcgaacctgg cctggtacca gcagaagcag      120

ggcaagagcc ctcagctgct ggtgtacatc gccattaacc tggcggacgg cgtaccctct      180

cggttttcag ggagcggctc ggggacccag tacagtctaa aaattaattc ccttcagtcc      240

gaagatttcg gcaactatta ctgtcagcac ttttggggca ctccgcgcac cttcggcgga      300

ggtaccaagc tggagatcaa g                                                321


<210>  16
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8 Antibody LC

<400>  16

Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu Ser Val Ser Val Gly 
1               5                   10                  15      


Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Glu Asn Ile Tyr Ser Asn 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser Pro Gln Leu Leu Val 
        35                  40                  45              


Tyr Ile Ala Ile Asn Leu Ala Asp Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile Asn Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe Trp Gly Thr Pro Arg 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  17
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR1

<400>  17
agctacatct tgcat                                                        15


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR1

<400>  18

Ser Tyr Ile Leu His 
1               5   


<210>  19
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR2

<400>  19
tatattaacc cgtacaacga cgccaccaag tacaacgaga aatttaaggg c                51


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR2

<400>  20

Tyr Ile Asn Pro Tyr Asn Asp Ala Thr Lys Tyr Asn Glu Lys Phe Lys 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR3

<400>  21
acaccacagc ctat                                                         14


<210>  22
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC CDR3

<400>  22

Ile Thr Thr Ala Tyr 
1               5   


<210>  23
<211>  354
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC

<400>  23
gaggtgcagc tgcagcagag cgggcccgag ctggtgaatc caggcgcgtc agtgaagatg       60

tcatgcaaag cttctggcta ctccttcacc agctacatct tgcattgggt caagcagaag      120

cctggacagg gtctggagtg gatcggttat attaacccgt acaacgacgc caccaagtac      180

aacgagaaat ttaagggcaa ggccacgctc actagcgata aaagctcgtc cacggcctac      240

atggaattga gttccgtcac ctccgaggac agcgcggtgt actactgttg ctctatgatc      300

accacagcct attgggcgta ctggggccag ggcactcttg ttacagtatc tgct            354


<210>  24
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody HC

<400>  24

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Asn Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Ser Tyr 
            20                  25                  30          


Ile Leu His Trp Val Lys Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Ile Asn Pro Tyr Asn Asp Ala Thr Lys Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Ser Ser Val Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Cys Ser Met Ile Thr Thr Ala Tyr Trp Ala Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Leu Val Thr Val Ser Ala 
        115             


<210>  25
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR1

<400>  25
aaatcctctc aatctatcct gtacggctcg acccagaaga actacctggc a                51


<210>  26
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR1

<400>  26

Lys Ser Ser Gln Ser Ile Leu Tyr Gly Ser Thr Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR2

<400>  27
tgggcttcca ctcgtgagag c                                                 21


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR2

<400>  28

Trp Ala Ser Thr Arg Glu Ser 
1               5           


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR3

<400>  29
caccagtacc tttcgagcta cacc                                              24


<210>  30
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC CDR3

<400>  30

His Gln Tyr Leu Ser Ser Tyr Thr 
1               5               


<210>  31
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC

<400>  31
aacatcatga tgacccagag cccgtcgtcc ctcaccgtgt ccgctggcga gaaggtgacc       60

atgtcttgca aatcctctca atctatcctg tacggctcga cccagaagaa ctacctggca      120

tggtaccagc agaagcccgg gcagagccct aagctgctga tttattgggc ttccactcgt      180

gagagcgggg tccccgaccg cttcaccggc tccggctccg gcaccgactt caccctgacc      240

atctcttccg tgcaggccga agatctggcc gtgtattact gtcaccagta cctttcgagc      300

tacaccttcg gcggtggcac taagttagag atcaag                                336


<210>  32
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2 Antibody LC

<400>  32

Asn Ile Met Met Thr Gln Ser Pro Ser Ser Leu Thr Val Ser Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Ile Leu Tyr Gly 
            20                  25                  30          


Ser Thr Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Glu Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val Tyr Tyr Cys His Gln 
                85                  90                  95      


Tyr Leu Ser Ser Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  33
<211>  1593
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8-28BBz_CAR

<400>  33
atggcattgc ctgttacagc tctgctgctg cccctggctc tgcttctgca tgctgccaga       60

cctgacatcc agatgaccca gagccctgct agtctctccg tgtccgttgg cgagacggtg      120

accatcacct gccgcgcatc cgagaacatc tactcgaacc tggcctggta ccagcagaag      180

cagggcaaga gccctcagct gctggtgtac atcgccatta acctggcgga cggcgtaccc      240

tctcggtttt cagggagcgg ctcggggacc cagtacagtc taaaaattaa ttcccttcag      300

tccgaagatt tcggcaacta ttactgtcag cacttttggg gcactccgcg caccttcggc      360

ggaggtacca agctggagat caagtcgggc ggaggaggca gcggcggcgg gggttccggt      420

ggaggcggct ctggcggcgg gggttctcag atccagcttg tgcagagcgg ccccgagctg      480

aagaagcccg gggagactgt caagatctct tgcaaggcgt ccggctacac gttcaccaac      540

tacggtatga actgggtgaa gcaggccccg gggcgtggct tgaaatggat gggttggatc      600

aatacttaca ccggtaatcc aacctacgcg gatgacttca agggccgctt cgatttttcg      660

ctggagacct ccgctagcac tgcctacctg caaattaaca acctcaaaaa cgaggacatg      720

gccatctatt tctgtgctcg cgacaggggc aactccttcg actattgggg ccagggtacc      780

acactgaccg tctcttctac aacaacccct gctcctcggc ctcctacacc agctcctaca      840

attgccagcc agcctctgtc tctgaggccc gaagcttgta gacctgctgc tggcggagcc      900

gtgcatacaa gaggactgga tttcgcctgc gacatctaca tctgggctcc tctggccgga      960

acatgtggcg tgctgctgct gagcctggtc atcaccctgt actgccggtc caagagaagc     1020

agactgctgc acagcgacta catgaacatg acccctagac ggcccggacc taccagaaag     1080

cactaccagc cttacgctcc tcctcgggac ttcgctgcct acagaagcaa gcggggcaga     1140

aagaagctgc tgtacatctt caagcagccc ttcatgcggc ccgtgcagac cacacaagag     1200

gaagatggct gctcctgcag attccccgag gaagaagaag gcggctgcga gctgagagtg     1260

aagttcagca gatccgctga cgcccctgcc tacaagcagg gacagaacca gctgtacaac     1320

gagctgaacc tggggagaag agaagagtac gacgtgctgg acaagcggag aggcagagat     1380

cctgagatgg gcggcaagcc cagacggaag aatcctcaag agggcctgta taatgagctg     1440

cagaaagaca agatggccga ggcctacagc gagatcggaa tgaagggcga gcgcagaaga     1500

ggcaagggac acgatggact gtaccagggc ctgagcaccg ccaccaagga tacctatgat     1560

gccctgcaca tgcaggccct gcctccaaga tag                                  1593


<210>  34
<211>  530
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 6D8-28BBz_CAR

<400>  34

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Ser Pro Ala Ser Leu 
            20                  25                  30          


Ser Val Ser Val Gly Glu Thr Val Thr Ile Thr Cys Arg Ala Ser Glu 
        35                  40                  45              


Asn Ile Tyr Ser Asn Leu Ala Trp Tyr Gln Gln Lys Gln Gly Lys Ser 
    50                  55                  60                  


Pro Gln Leu Leu Val Tyr Ile Ala Ile Asn Leu Ala Asp Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Gln Tyr Ser Leu Lys Ile 
                85                  90                  95      


Asn Ser Leu Gln Ser Glu Asp Phe Gly Asn Tyr Tyr Cys Gln His Phe 
            100                 105                 110         


Trp Gly Thr Pro Arg Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gln Ile Gln Leu Val Gln Ser Gly Pro Glu Leu 
145                 150                 155                 160 


Lys Lys Pro Gly Glu Thr Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr 
                165                 170                 175     


Thr Phe Thr Asn Tyr Gly Met Asn Trp Val Lys Gln Ala Pro Gly Arg 
            180                 185                 190         


Gly Leu Lys Trp Met Gly Trp Ile Asn Thr Tyr Thr Gly Asn Pro Thr 
        195                 200                 205             


Tyr Ala Asp Asp Phe Lys Gly Arg Phe Asp Phe Ser Leu Glu Thr Ser 
    210                 215                 220                 


Ala Ser Thr Ala Tyr Leu Gln Ile Asn Asn Leu Lys Asn Glu Asp Met 
225                 230                 235                 240 


Ala Ile Tyr Phe Cys Ala Arg Asp Arg Gly Asn Ser Phe Asp Tyr Trp 
                245                 250                 255     


Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Thr Thr Thr Pro Ala Pro 
            260                 265                 270         


Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu 
        275                 280                 285             


Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg 
    290                 295                 300                 


Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly 
305                 310                 315                 320 


Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Arg 
                325                 330                 335     


Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro 
            340                 345                 350         


Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro 
        355                 360                 365             


Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg Gly Arg Lys Lys Leu Leu 
    370                 375                 380                 


Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu 
385                 390                 395                 400 


Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys 
                405                 410                 415     


Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys 
            420                 425                 430         


Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
        435                 440                 445             


Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
    450                 455                 460                 


Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
465                 470                 475                 480 


Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
                485                 490                 495     


Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
            500                 505                 510         


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
        515                 520                 525             


Pro Arg 
    530 


<210>  35
<211>  1611
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2-28BBz_CAR

<400>  35
atggcattgc ctgttacagc tctgctgctg cccctggctc tgcttctgca tgctgccaga       60

cctaacatca tgatgaccca gagcccgtcg tccctcaccg tgtccgctgg cgagaaggtg      120

accatgtctt gcaaatcctc tcaatctatc ctgtacggct cgacccagaa gaactacctg      180

gcatggtacc agcagaagcc cgggcagagc cctaagctgc tgatttattg ggcttccact      240

cgtgagagcg gggtccccga ccgcttcacc ggctccggct ccggcaccga cttcaccctg      300

accatctctt ccgtgcaggc cgaagatctg gccgtgtatt actgtcacca gtacctttcg      360

agctacacct tcggcggtgg cactaagtta gagatcaagt cgggcggggg aggaagtggc      420

gggggtggtt ctggcggcgg tggttccggc ggaggagggt ccgaggtgca gctgcagcag      480

agcgggcccg agctggtgaa tccaggcgcg tcagtgaaga tgtcatgcaa agcttctggc      540

tactccttca ccagctacat cttgcattgg gtcaagcaga agcctggaca gggtctggag      600

tggatcggtt atattaaccc gtacaacgac gccaccaagt acaacgagaa atttaagggc      660

aaggccacgc tcactagcga taaaagctcg tccacggcct acatggaatt gagttccgtc      720

acctccgagg acagcgcggt gtactactgt tgctctatga tcaccacagc ctattgggcg      780

tactggggcc agggcactct tgttacagta tctgctacaa caacccctgc tcctcggcct      840

cctacaccag ctcctacaat tgccagccag cctctgtctc tgaggcccga agcttgtaga      900

cctgctgctg gcggagccgt gcatacaaga ggactggatt tcgcctgcga catctacatc      960

tgggctcctc tggccggaac atgtggcgtg ctgctgctga gcctggtcat caccctgtac     1020

tgccggtcca agagaagcag actgctgcac agcgactaca tgaacatgac ccctagacgg     1080

cccggaccta ccagaaagca ctaccagcct tacgctcctc ctcgggactt cgctgcctac     1140

agaagcaagc ggggcagaaa gaagctgctg tacatcttca agcagccctt catgcggccc     1200

gtgcagacca cacaagagga agatggctgc tcctgcagat tccccgagga agaagaaggc     1260

ggctgcgagc tgagagtgaa gttcagcaga tccgctgacg cccctgccta caagcaggga     1320

cagaaccagc tgtacaacga gctgaacctg gggagaagag aagagtacga cgtgctggac     1380

aagcggagag gcagagatcc tgagatgggc ggcaagccca gacggaagaa tcctcaagag     1440

ggcctgtata atgagctgca gaaagacaag atggccgagg cctacagcga gatcggaatg     1500

aagggcgagc gcagaagagg caagggacac gatggactgt accagggcct gagcaccgcc     1560

accaaggata cctatgatgc cctgcacatg caggccctgc ctccaagata g              1611


<210>  36
<211>  536
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, 10D2-28BBz_CAR

<400>  36

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asn Ile Met Met Thr Gln Ser Pro Ser Ser Leu 
            20                  25                  30          


Thr Val Ser Ala Gly Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln 
        35                  40                  45              


Ser Ile Leu Tyr Gly Ser Thr Gln Lys Asn Tyr Leu Ala Trp Tyr Gln 
    50                  55                  60                  


Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr 
65                  70                  75                  80  


Arg Glu Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr 
                85                  90                  95      


Asp Phe Thr Leu Thr Ile Ser Ser Val Gln Ala Glu Asp Leu Ala Val 
            100                 105                 110         


Tyr Tyr Cys His Gln Tyr Leu Ser Ser Tyr Thr Phe Gly Gly Gly Thr 
        115                 120                 125             


Lys Leu Glu Ile Lys Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Gln Gln 
145                 150                 155                 160 


Ser Gly Pro Glu Leu Val Asn Pro Gly Ala Ser Val Lys Met Ser Cys 
                165                 170                 175     


Lys Ala Ser Gly Tyr Ser Phe Thr Ser Tyr Ile Leu His Trp Val Lys 
            180                 185                 190         


Gln Lys Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile Asn Pro Tyr 
        195                 200                 205             


Asn Asp Ala Thr Lys Tyr Asn Glu Lys Phe Lys Gly Lys Ala Thr Leu 
    210                 215                 220                 


Thr Ser Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Ser Ser Val 
225                 230                 235                 240 


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Cys Ser Met Ile Thr Thr 
                245                 250                 255     


Ala Tyr Trp Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ala 
            260                 265                 270         


Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
        275                 280                 285             


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
    290                 295                 300                 


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
305                 310                 315                 320 


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
                325                 330                 335     


Ile Thr Leu Tyr Cys Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Lys Arg 
    370                 375                 380                 


Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro 
385                 390                 395                 400 


Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu 
                405                 410                 415     


Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala 
            420                 425                 430         


Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu 
        435                 440                 445             


Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly 
    450                 455                 460                 


Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu 
465                 470                 475                 480 


Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser 
                485                 490                 495     


Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly 
            500                 505                 510         


Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu 
        515                 520                 525             


His Met Gln Ala Leu Pro Pro Arg 
    530                 535     


