                               SEQUENCE LISTING

<110> PRESIDENT AND FELLOWS OF HARVARD COLLEGE
      MASSACHUSETTS INSTITUTE OF TECHNOLOGY
 
<120> ENGINEERED PROBIOTIC COMPOSITIONS AND USES THEREOF

<130> 002806-098940WOPT

<140> PCT/US2021/061414
<141> 2021-12-01

<150> 63/119,772
<151> 2020-12-01

<160> 10    

<170> PatentIn version 3.5

<210> 1
<211> 516
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 1
atgcatcctg aaacattggt aaaggtaaag gacgcagagg accagttagg tgcccgtgtg       60

ggatacatcg agttggatct taattctggg aaaattttag agagcttccg tccggaggaa      120

cgattcccga tgatgtcaac ttttaaggta cttttgtgcg gggccgtttt gagccgtatc      180

gacgctggac aggagcagtt aggacgacga atacattact cacagaatga ccttgtggaa      240

tacagtcctg ttaccgagaa acaccttacc gatgggatga cagtacgtga gttatgctct      300

gctgcaataa cgatgtctga caacacggca gcaaacttat tacttacaac cattggaggg      360

ccaaaggaat taaccgcctt tttgcacaat atgggcgatc atgtaaccag attggataga      420

tgggagccgg agttgaacga agcaattcca aacgatgaga gagatactac gactcctgta      480

gcaatggcga ccactttgcg aaaacttttg acggga                                516


<210> 2
<211> 172
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Met His Pro Glu Thr Leu Val Lys Val Lys Asp Ala Glu Asp Gln Leu 
1               5                   10                  15      


Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp Leu Asn Ser Gly Lys Ile 
            20                  25                  30          


Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe Pro Met Met Ser Thr Phe 
        35                  40                  45              


Lys Val Leu Leu Cys Gly Ala Val Leu Ser Arg Ile Asp Ala Gly Gln 
    50                  55                  60                  


Glu Gln Leu Gly Arg Arg Ile His Tyr Ser Gln Asn Asp Leu Val Glu 
65                  70                  75                  80  


Tyr Ser Pro Val Thr Glu Lys His Leu Thr Asp Gly Met Thr Val Arg 
                85                  90                  95      


Glu Leu Cys Ser Ala Ala Ile Thr Met Ser Asp Asn Thr Ala Ala Asn 
            100                 105                 110         


Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys Glu Leu Thr Ala Phe Leu 
        115                 120                 125             


His Asn Met Gly Asp His Val Thr Arg Leu Asp Arg Trp Glu Pro Glu 
    130                 135                 140                 


Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg Asp Thr Thr Thr Pro Val 
145                 150                 155                 160 


Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly 
                165                 170         


<210> 3
<211> 666
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 3
atgaaaaaaa agattattag tgcgatcctt atgagtactg ttatccttag tgctgccgcc       60

ccgctttctg gggtctatgc tgcgcatatt gttatggtgg acgcctataa gcctacgaaa      120

ggagggggtg gatcaggtgg cggcgggtct atgcatcctg aaacattggt aaaggtaaag      180

gacgcagagg accagttagg tgcccgtgtg ggatacatcg agttggatct taattctggg      240

aaaattttag agagcttccg tccggaggaa cgattcccga tgatgtcaac ttttaaggta      300

cttttgtgcg gggccgtttt gagccgtatc gacgctggac aggagcagtt aggacgacga      360

atacattact cacagaatga ccttgtggaa tacagtcctg ttaccgagaa acaccttacc      420

gatgggatga cagtacgtga gttatgctct gctgcaataa cgatgtctga caacacggca      480

gcaaacttat tacttacaac cattggaggg ccaaaggaat taaccgcctt tttgcacaat      540

atgggcgatc atgtaaccag attggataga tgggagccgg agttgaacga agcaattcca      600

aacgatgaga gagatactac gactcctgta gcaatggcga ccactttgcg aaaacttttg      660

acggga                                                                 666


<210> 4
<211> 222
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Ala His Ile Val Met 
            20                  25                  30          


Val Asp Ala Tyr Lys Pro Thr Lys Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Met His Pro Glu Thr Leu Val Lys Val Lys Asp Ala Glu Asp 
    50                  55                  60                  


Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp Leu Asn Ser Gly 
65                  70                  75                  80  


Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe Pro Met Met Ser 
                85                  90                  95      


Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser Arg Ile Asp Ala 
            100                 105                 110         


Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser Gln Asn Asp Leu 
        115                 120                 125             


Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr Asp Gly Met Thr 
    130                 135                 140                 


Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser Asp Asn Thr Ala 
145                 150                 155                 160 


Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys Glu Leu Thr Ala 
                165                 170                 175     


Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu Asp Arg Trp Glu 
            180                 185                 190         


Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg Asp Thr Thr Thr 
        195                 200                 205             


Pro Val Ala Met Ala Thr Thr Leu Arg Lys Leu Leu Thr Gly 
    210                 215                 220         


<210> 5
<211> 273
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
ttacttactt tagcatctcg acaacagtta atagactgga tggaagccga caaagtggcc       60

ggtccgcttt tgcgatctgc attgccagca gggtggttca tcgccgataa atcaggcgcc      120

ggggaaagag gaagccgtgg catcatcgcc gccttaggtc cagacggtaa accgagtcga      180

attgtagtca tatacacgac ggggagtcag gcaactatgg acgagagaaa ccgacagatt      240

gccgagatcg gggccagttt aataaaacat tgg                                   273


<210> 6
<211> 91
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp Met Glu Ala 
1               5                   10                  15      


Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro Ala Gly Trp 
            20                  25                  30          


Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser Arg Gly Ile 
        35                  40                  45              


Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile Val Val Ile 
    50                  55                  60                  


Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn Arg Gln Ile 
65                  70                  75                  80  


Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp 
                85                  90      


<210> 7
<211> 732
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 7
atgaaaaaaa agattattag tgcgatcctt atgagtactg ttatccttag tgctgccgcc       60

ccgctttctg gggtctatgc tggtgcgatg gttgatacct tgtctggcct tagttctgag      120

caagggcaga gtggggatat gacaatagag gaggatagcg ccacccacat taagttcagt      180

aaacgtgatg aggatggaaa agaattggcg ggagccacga tggaacttag agacagcagt      240

gggaaaacga tttctacgtg gatcagcgac ggccaggtta aggatttcta tctttatcct      300

ggaaagtaca ctttcgttga aacagcagcg cctgacggat acgaagtcgc gacagcaata      360

acctttacag taaatgaaca gggacaagtg actgtaaatg gtaaggcgac taaaggggat      420

gcccatatag gagggggtgg atcaggtggc ggcgggtctt tacttacttt agcatctcga      480

caacagttaa tagactggat ggaagccgac aaagtggccg gtccgctttt gcgatctgca      540

ttgccagcag ggtggttcat cgccgataaa tcaggcgccg gggaaagagg aagccgtggc      600

atcatcgccg ccttaggtcc agacggtaaa ccgagtcgaa ttgtagtcat atacacgacg      660

gggagtcagg caactatgga cgagagaaac cgacagattg ccgagatcgg ggccagttta      720

ataaaacatt gg                                                          732


<210> 8
<211> 244
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Lys Lys Lys Ile Ile Ser Ala Ile Leu Met Ser Thr Val Ile Leu 
1               5                   10                  15      


Ser Ala Ala Ala Pro Leu Ser Gly Val Tyr Ala Gly Ala Met Val Asp 
            20                  25                  30          


Thr Leu Ser Gly Leu Ser Ser Glu Gln Gly Gln Ser Gly Asp Met Thr 
        35                  40                  45              


Ile Glu Glu Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu 
    50                  55                  60                  


Asp Gly Lys Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser 
65                  70                  75                  80  


Gly Lys Thr Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe 
                85                  90                  95      


Tyr Leu Tyr Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp 
            100                 105                 110         


Gly Tyr Glu Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly 
        115                 120                 125             


Gln Val Thr Val Asn Gly Lys Ala Thr Lys Gly Asp Ala His Ile Gly 
    130                 135                 140                 


Gly Gly Gly Ser Gly Gly Gly Gly Ser Leu Leu Thr Leu Ala Ser Arg 
145                 150                 155                 160 


Gln Gln Leu Ile Asp Trp Met Glu Ala Asp Lys Val Ala Gly Pro Leu 
                165                 170                 175     


Leu Arg Ser Ala Leu Pro Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly 
            180                 185                 190         


Ala Gly Glu Arg Gly Ser Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp 
        195                 200                 205             


Gly Lys Pro Ser Arg Ile Val Val Ile Tyr Thr Thr Gly Ser Gln Ala 
    210                 215                 220                 


Thr Met Asp Glu Arg Asn Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu 
225                 230                 235                 240 


Ile Lys His Trp 
                


<210> 9
<211> 19
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 9
gtgccagcmg ccgcggtaa                                                    19


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 10
ggactachvg ggtwtctaat                                                   20


