                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  A Highly Sensitive Heat-Repressible Split-T7 Polymerase 
       (Thermal-T7RNAP) For Applications In Biotechnology

<130>  SP103248WO

<150>  SG10202012000X
<151>  2020-12-02

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  883
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T7RNAP

<400>  1

Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      


Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          


Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              


Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  


Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  


Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      


Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         


Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             


Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 


Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 


His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     


Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         


Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             


Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 


Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 


Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     


Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         


Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             


Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 


Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
305                 310                 315                 320 


Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
                325                 330                 335     


Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         


Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
        355                 360                 365             


Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 


Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 


Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     


Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         


Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             


Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 


Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 


Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     


Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         


Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             


Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 


His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 


Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     


Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         


Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             


Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 


Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 


Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     


Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         


Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             


Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 


Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 


Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     


Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         


Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             


Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 


Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 


Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     


Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         


Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             


Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 


Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 


Ala Phe Ala 
            


<210>  2
<211>  371
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tlpa36

<400>  2

Met Arg Pro Ala Thr Tyr Glu Pro Glu Gln Ile Ile Glu Ala Gly Leu 
1               5                   10                  15      


Ala Leu Gln Ala Glu Gly Arg Asn Ile Thr Gly Phe Ala Leu Arg Asn 
            20                  25                  30          


Gln Val Gly Gly Gly Asn Pro Thr Arg Leu Arg Gln Ile Trp Asp Glu 
        35                  40                  45              


Tyr Gln Ala Ser Gln Ser Thr Val Val Thr Glu Leu Val Ala Glu Leu 
    50                  55                  60                  


Pro Val Glu Val Ala Glu Glu Val Lys Ala Val Ser Ala Ala Leu Ser 
65                  70                  75                  80  


Glu Arg Ile Thr Gln Leu Ala Thr Glu Leu Asn Asp Lys Ala Val Arg 
                85                  90                  95      


Ala Ala Glu Arg Arg Val Ala Glu Val Thr Arg Ala Ala Gly Glu Gln 
            100                 105                 110         


Thr Ala Gln Ala Glu Arg Glu Leu Ala Asp Ala Ala Gln Thr Val Asp 
        115                 120                 125             


Asp Leu Glu Glu Lys Leu Val Glu Leu Gln Asp Arg Tyr Asp Ser Leu 
    130                 135                 140                 


Thr Leu Ala Leu Glu Ser Glu Arg Ser Leu Arg Gln Gln His Asp Val 
145                 150                 155                 160 


Glu Met Ala Gln Leu Lys Glu Arg Leu Ala Ala Ala Glu Glu Asn Thr 
                165                 170                 175     


Arg Gln Arg Glu Glu Arg Tyr Gln Glu Gln Arg Thr Val Leu Gln Asp 
            180                 185                 190         


Ala Leu Asn Ala Glu Gln Ala Gln His Ile Asn Thr Arg Glu Asp Gln 
        195                 200                 205             


Gln Lys Arg Leu Glu Gln Ile Ser Ala Glu Ala Asn Ala Arg Thr Glu 
    210                 215                 220                 


Glu Leu Lys Ser Glu Arg Asp Lys Val Asn Thr Leu Leu Thr Arg Leu 
225                 230                 235                 240 


Glu Ser Gln Glu Asn Ala Leu Ala Ser Glu Arg Gln Gln His Leu Ala 
                245                 250                 255     


Thr Arg Glu Thr Leu Gln Gln Arg Leu Glu Gln Ala Ile Ala Asp Thr 
            260                 265                 270         


Gln Ala Arg Ala Gly Glu Ile Ala Leu Glu Arg Asp Arg Val Ser Ser 
        275                 280                 285             


Leu Thr Ala Arg Leu Glu Ser Gln Glu Lys Ala Ser Ser Glu Gln Leu 
    290                 295                 300                 


Val Arg Met Gly Ser Glu Ile Ala Ser Leu Thr Glu Arg Cys Thr Gln 
305                 310                 315                 320 


Leu Glu Asn Gln Arg Asp Asp Ala Arg Leu Glu Thr Met Gly Glu Lys 
                325                 330                 335     


Glu Thr Val Ala Ala Leu Arg Gly Glu Ala Glu Ala Leu Lys Arg Gln 
            340                 345                 350         


Asn Gln Ser Leu Met Ala Ala Leu Ser Gly Asn Lys Gln Thr Gly Gly 
        355                 360                 365             


Gln Asn Ala 
    370     


<210>  3
<211>  278
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Tlpa36 coiled coil domain

<400>  3

Ala Val Arg Ala Ala Glu Arg Arg Val Ala Glu Val Thr Arg Ala Ala 
1               5                   10                  15      


Gly Glu Gln Thr Ala Gln Ala Glu Arg Glu Leu Ala Asp Ala Ala Gln 
            20                  25                  30          


Thr Val Asp Asp Leu Glu Glu Lys Leu Val Glu Leu Gln Asp Arg Tyr 
        35                  40                  45              


Asp Ser Leu Thr Leu Ala Leu Glu Ser Glu Arg Ser Leu Arg Gln Gln 
    50                  55                  60                  


His Asp Val Glu Met Ala Gln Leu Lys Glu Arg Leu Ala Ala Ala Glu 
65                  70                  75                  80  


Glu Asn Thr Arg Gln Arg Glu Glu Arg Tyr Gln Glu Gln Arg Thr Val 
                85                  90                  95      


Leu Gln Asp Ala Leu Asn Ala Glu Gln Ala Gln His Ile Asn Thr Arg 
            100                 105                 110         


Glu Asp Gln Gln Lys Arg Leu Glu Gln Ile Ser Ala Glu Ala Asn Ala 
        115                 120                 125             


Arg Thr Glu Glu Leu Lys Ser Glu Arg Asp Lys Val Asn Thr Leu Leu 
    130                 135                 140                 


Thr Arg Leu Glu Ser Gln Glu Asn Ala Leu Ala Ser Glu Arg Gln Gln 
145                 150                 155                 160 


His Leu Ala Thr Arg Glu Thr Leu Gln Gln Arg Leu Glu Gln Ala Ile 
                165                 170                 175     


Ala Asp Thr Gln Ala Arg Ala Gly Glu Ile Ala Leu Glu Arg Asp Arg 
            180                 185                 190         


Val Ser Ser Leu Thr Ala Arg Leu Glu Ser Gln Glu Lys Ala Ser Ser 
        195                 200                 205             


Glu Gln Leu Val Arg Met Gly Ser Glu Ile Ala Ser Leu Thr Glu Arg 
    210                 215                 220                 


Cys Thr Gln Leu Glu Asn Gln Arg Asp Asp Ala Arg Leu Glu Thr Met 
225                 230                 235                 240 


Gly Glu Lys Glu Thr Val Ala Ala Leu Arg Gly Glu Ala Glu Ala Leu 
                245                 250                 255     


Lys Arg Gln Asn Gln Ser Leu Met Ala Ala Leu Ser Gly Asn Lys Gln 
            260                 265                 270         


Thr Gly Gly Gln Asn Ala 
        275             


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pT7

<400>  4
taatacgact cactataggg                                                   20


<210>  5
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  5
ggcggttctg gaggt                                                        15


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RbsDefault

<400>  6
tttaagaagg agatatacat                                                   20


<210>  7
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gabDP2

<400>  7
gagattttgg gctcgtcggg gattcgccgg gtgctgcaaa accatctacg ctcaggactg       60

ggcgagatga aaaactcgct g                                                 81


<210>  8
<211>  627
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fcs

<400>  8

Met Asn Asn Glu Ala Arg Ser Gly Ser Thr Asp Pro Gly Gln Arg Pro 
1               5                   10                  15      


Arg Tyr Arg Gln Val Ala Ile Gly His Pro Gln Val Gln Val Ser His 
            20                  25                  30          


Val Asp Asp Val Leu Arg Met Gln Pro Val Glu Pro Leu Ala Pro Leu 
        35                  40                  45              


Pro Ala Arg Leu Leu Glu Arg Leu Val His Trp Ala Gln Val Arg Pro 
    50                  55                  60                  


Asp Thr Thr Phe Ile Ala Ala Arg Gln Ala Asp Gly Ala Trp Arg Ser 
65                  70                  75                  80  


Ile Ser Tyr Val Gln Met Leu Ala Asp Val Arg Thr Ile Ala Ala Asn 
                85                  90                  95      


Leu Leu Gly Leu Gly Leu Ser Ala Glu Arg Pro Leu Ala Leu Leu Ser 
            100                 105                 110         


Gly Asn Asp Ile Glu His Leu Gln Ile Ala Leu Gly Ala Met Tyr Ala 
        115                 120                 125             


Gly Ile Ala Tyr Cys Pro Val Ser Pro Ala Tyr Ala Leu Leu Ser Gln 
    130                 135                 140                 


Asp Phe Ala Lys Leu Arg His Val Cys Glu Val Leu Thr Pro Gly Val 
145                 150                 155                 160 


Val Phe Val Ser Asp Ser Gln Pro Phe Gln Arg Ala Phe Glu Ala Val 
                165                 170                 175     


Leu Asp Asp Ser Val Gly Val Ile Ser Val Arg Gly Gln Val Ala Gly 
            180                 185                 190         


Arg Pro His Ile Ser Phe Asp Ser Leu Leu Gln Pro Gly Asp Leu Ala 
        195                 200                 205             


Ala Ala Asp Ala Ala Phe Ala Ala Thr Gly Pro Asp Thr Ile Ala Lys 
    210                 215                 220                 


Phe Leu Phe Thr Ser Gly Ser Thr Lys Leu Pro Lys Ala Val Ile Thr 
225                 230                 235                 240 


Thr Gln Arg Met Leu Cys Ala Asn Gln Gln Met Leu Leu Gln Thr Phe 
                245                 250                 255     


Pro Thr Phe Ala Glu Glu Pro Pro Val Leu Val Asp Trp Leu Pro Trp 
            260                 265                 270         


Asn His Thr Phe Gly Gly Ser His Asn Leu Gly Ile Val Leu Tyr Asn 
        275                 280                 285             


Gly Gly Ser Phe Tyr Leu Asp Ala Gly Lys Pro Thr Pro Gln Gly Phe 
    290                 295                 300                 


Ala Glu Thr Leu Arg Asn Leu Arg Glu Ile Ser Pro Thr Ala Tyr Leu 
305                 310                 315                 320 


Thr Val Pro Lys Gly Trp Glu Glu Leu Val Lys Ala Leu Glu Gln Asp 
                325                 330                 335     


Pro Ala Leu Arg Glu Val Phe Phe Ala Arg Ile Lys Leu Phe Phe Phe 
            340                 345                 350         


Ala Ala Ala Gly Leu Ser Gln Ser Val Trp Asp Arg Leu Asp Arg Ile 
        355                 360                 365             


Ala Glu Gln His Cys Gly Glu Arg Ile Arg Met Met Ala Gly Leu Gly 
    370                 375                 380                 


Met Thr Glu Ala Ser Pro Ser Cys Thr Phe Thr Thr Gly Pro Leu Ser 
385                 390                 395                 400 


Met Ala Gly Tyr Val Gly Leu Pro Ala Pro Gly Cys Glu Val Lys Leu 
                405                 410                 415     


Val Pro Val Gly Asp Lys Leu Glu Ala Arg Phe Arg Gly Pro His Ile 
            420                 425                 430         


Met Pro Gly Tyr Trp Arg Ser Pro Gln Gln Thr Ala Glu Ala Phe Asp 
        435                 440                 445             


Glu Glu Gly Phe Tyr Cys Ser Gly Asp Ala Leu Lys Leu Ala Asp Ala 
    450                 455                 460                 


Arg Gln Pro Glu Leu Gly Leu Met Phe Asp Gly Arg Ile Ala Glu Asp 
465                 470                 475                 480 


Phe Lys Leu Ser Ser Gly Val Phe Val Ser Val Gly Pro Leu Arg Asn 
                485                 490                 495     


Arg Ala Val Leu Glu Gly Ser Pro Tyr Val Gln Asp Ile Val Val Thr 
            500                 505                 510         


Ala Pro Asp Arg Glu Cys Leu Gly Leu Leu Val Phe Pro Arg Leu Pro 
        515                 520                 525             


Glu Cys Arg Arg Leu Ala Gly Leu Ala Glu Asp Ala Ser Asp Ala Arg 
    530                 535                 540                 


Val Leu Ala Asn Asp Thr Val Arg Ser Trp Phe Ala Asp Trp Leu Glu 
545                 550                 555                 560 


Arg Leu Asn Arg Asp Ala Gln Gly Asn Ala Ser Arg Ile Glu Trp Leu 
                565                 570                 575     


Ser Leu Leu Ala Glu Pro Pro Ser Ile Asp Ala Gly Glu Ile Thr Asp 
            580                 585                 590         


Lys Gly Ser Ile Asn Gln Arg Ala Val Leu Gln Arg Arg Ala Ala Gln 
        595                 600                 605             


Val Glu Ala Leu Tyr Arg Gly Glu Asp Pro Asp Ala Leu His Ala Lys 
    610                 615                 620                 


Val Arg Pro 
625         


<210>  9
<211>  276
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ech

<400>  9

Met Ser Lys Tyr Glu Gly Arg Trp Thr Thr Val Lys Val Glu Leu Glu 
1               5                   10                  15      


Ala Gly Ile Ala Trp Val Thr Leu Asn Arg Pro Glu Lys Arg Asn Ala 
            20                  25                  30          


Met Ser Pro Thr Leu Asn Arg Glu Met Val Asp Val Leu Glu Thr Leu 
        35                  40                  45              


Glu Gln Asp Ala Asp Ala Gly Val Leu Val Leu Thr Gly Ala Gly Glu 
    50                  55                  60                  


Ser Trp Thr Ala Gly Met Asp Leu Lys Glu Tyr Phe Arg Glu Val Asp 
65                  70                  75                  80  


Ala Gly Pro Glu Ile Leu Gln Glu Lys Ile Arg Arg Glu Ala Ser Gln 
                85                  90                  95      


Trp Gln Trp Lys Leu Leu Arg Leu Tyr Ala Lys Pro Thr Ile Ala Met 
            100                 105                 110         


Val Asn Gly Trp Cys Phe Gly Gly Gly Phe Ser Pro Leu Val Ala Cys 
        115                 120                 125             


Asp Leu Ala Ile Cys Ala Asn Glu Ala Thr Phe Gly Leu Ser Glu Ile 
    130                 135                 140                 


Asn Trp Gly Ile Pro Pro Gly Asn Leu Val Ser Lys Ala Met Ala Asp 
145                 150                 155                 160 


Thr Val Gly His Arg Gln Ser Leu Tyr Tyr Ile Met Thr Gly Lys Thr 
                165                 170                 175     


Phe Asp Gly Arg Lys Ala Ala Glu Met Gly Leu Val Asn Asp Ser Val 
            180                 185                 190         


Pro Leu Ala Glu Leu Arg Glu Thr Thr Arg Glu Leu Ala Leu Asn Leu 
        195                 200                 205             


Leu Glu Lys Asn Pro Val Val Leu Arg Ala Ala Lys Asn Gly Phe Lys 
    210                 215                 220                 


Arg Cys Arg Glu Leu Thr Trp Glu Gln Asn Glu Asp Tyr Leu Tyr Ala 
225                 230                 235                 240 


Lys Leu Asp Gln Ser Arg Leu Leu Asp Thr Thr Gly Gly Arg Glu Gln 
                245                 250                 255     


Gly Met Lys Gln Phe Leu Asp Asp Lys Ser Ile Lys Pro Gly Leu Gln 
            260                 265                 270         


Ala Tyr Lys Arg 
        275     


<210>  10
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PTlpa

<400>  10
tttaatttgt ttgttagtta gtttatttgt tggtttgttt gtgttataat at               52


<210>  11
<211>  227
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SgrS

<400>  11
gatgaagcaa gggggtgccc catgcgtcag ttttatcagc actattttac cgcgacagcg       60

aagttgtgct ggttgcgttg gttaagcgtc ccacaacgat taaccatgct tgaaggactg      120

atgcagtggg atgaccgcaa ttctgaaagt tgacttgcct gcatcatgtg tgactgagta      180

ttggtgtaaa atcacccgcc agcagattat acctgctggt ttttttt                    227


<210>  12
<211>  423
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated coiled-coil domain of CT7 protein fragment (Mut1)

<400>  12
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg taaacagacc       60

ggtggccaga atgcgtaaca ataaacagac cggtggccag aatgcgtaaa tcgaggatta      120

ccactacgct acgtgtaaaa acgaggacct gatgcacttt gacgacttgg gatcgacgag      180

agcagcgcga cagaccggtg gccagaatgc gtaacaataa acagaccggt ggccagaatg      240

cgtaaatcga ggattaccac tacgctacgt gactacgctg atgtacagat ccatactgca      300

tgttcattga cgatctacaa cgcatcaccc agctggcgac agaacgcatc acccagctgg      360

cgacagaacc agtcactgat ggcggcgctt tcaggcaata aacagaccgg tggccagaat      420

gcg                                                                    423


<210>  13
<211>  270
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mutated coiled-coil domain of CT7 protein fragment (Mut2)

<400>  13
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg tgaacagacc       60

gcacaggcag agcgggagct ggccgacgcc gcgcagacag tcgacgacct ggaagaaaaa      120

ctggttgaac tgcaggacag atatgacagt ttgacgctgg cgctggagtc agaacgttca      180

ctgcgtggtg aggctgaagc cctgaagcgt cagaaccagt cactgatggc ggcgctttca      240

ggcaataaac agaccggtgg ccagaatgcg                                       270


<210>  14
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X1

<400>  14
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg tgaacagacc       60

g                                                                       61


<210>  15
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X2

<400>  15
cacaggcaga gcgggagctg gccgacgccg cgcagacagt cgacgacctg gaagaaaaac       60

tggttgaact gcaggacaga tatgacagtt tgacgctggc gctggagtca gaacgttcac      120

tgcgt                                                                  125


<210>  16
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X3

<400>  16
cactgcgtgg tgaggctgaa gccctga                                           27


<210>  17
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X4

<400>  17
gcgtcagaac cagtcactga tggcggcgct ttcaggcaat aaacagaccg gtggccagaa       60

tgcg                                                                    64


<210>  18
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X5

<400>  18
caataaacag accggtggcc agaatgcg                                          28


<210>  19
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tlpa positions 1050-1113

<400>  19
gcgtcagaac cagtcactga tggcggcgct ttcaggcaat aaacagaccg gtggccagaa       60

tgcg                                                                    64


<210>  20
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mut1 positions 360-423

<400>  20
gcgacagaac cagtcactga tggcggcgct ttcaggcaat aaacagaccg gtggccagaa       60

tgcg                                                                    64


<210>  21
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tlpa positions 280-340

<400>  21
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg tgaacagacc       60

g                                                                       61


<210>  22
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mut1 positions 1-61

<400>  22
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg taaacagacc       60

g                                                                       61


<210>  23
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tlpa positions 1086-1113

<400>  23
caataaacag accggtggcc agaatgcg                                          28


<210>  24
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tlpa positions 280-465

<400>  24
gcggtccggg ctgcagaacg ccgggttgcg gaagtcacgc gtgctgccgg tgaacagacc       60

gcacaggcag agcgggagct ggccgacgcc gcgcagacag tcgacgacct ggaagaaaaa      120

ctggttgaac tgcaggacag atatgacagt ttgacgctgg cgctggagtc agaacgttca      180

ctgcgt                                                                 186


<210>  25
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tlpa positions 1022-1113

<400>  25
cactgcgtgg tgaggctgaa gccctgaagc gtcagaacca gtcactgatg gcggcgcttt       60

caggcaataa acagaccggt ggccagaatg cg                                     92


<210>  26
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mut1 positions 341-465

<400>  26
cacaggcaga gcgggagctg gccgacgccg cgcagacagt cgacgacctg gaagaaaaac       60

tggttgaact gcaggacaga tatgacagtt tgacgctggc gctggagtca gaacgttcac      120

tgcgt                                                                  125


<210>  27
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mut1 positions 1022-1049

<400>  27
cactgcgtgg tgaggctgaa gccctgaa                                          28


