                         SEQUENCE LISTING

<110>  Planeat Foods PTE LTD.
 
<120>  METHOD OF PRODUCING GLOBIN POLYPEPTIDE RECOMBINANTLY AND MEAT 
       SUBSTITUTE FOOD PRODUCT

<130>  PLF17042PCT

<150>  EP20211249.6
<151>  2020-12-02

<160>  47    

<170>  PatentIn version 3.5

<210>  1
<211>  441
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA sequence of VsLegH

<400>  1
atgtctgttg ctttttctga taagcaagaa gcattggtta atggttcatg ggaagccttt       60

aaaactaaca tcccaaagta ctctgttgtt ttctatacta caattttaga aaaagctcca      120

gctgctaaaa atttgttttc atttttggca aatggtgttg atccatcaaa tccaaaattg      180

ttagctcatg cagaaaaatt gttcggttta gttagagatt ctgctgcaca attgagagct      240

tcaggtacag ttgttgcaga tgctgcatta ggttctgttc attcacaaaa agctgttact      300

gatgcacaat tcttggttgt taaagaagca ttggttaaga cattgaaaga agcagttggt      360

gacaaatggt cagatgaatt aactacagct gttgaattgg cttatgatga attagctgtt      420

gctattaaaa aggcttatta a                                                441


<210>  2
<211>  146
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of VsLegH

<400>  2

Met Ser Val Ala Phe Ser Asp Lys Gln Glu Ala Leu Val Asn Gly Ser 
1               5                   10                  15      


Trp Glu Ala Phe Lys Thr Asn Ile Pro Lys Tyr Ser Val Val Phe Tyr 
            20                  25                  30          


Thr Thr Ile Leu Glu Lys Ala Pro Ala Ala Lys Asn Leu Phe Ser Phe 
        35                  40                  45              


Leu Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu Leu Ala His Ala 
    50                  55                  60                  


Glu Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala Gln Leu Arg Ala 
65                  70                  75                  80  


Ser Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Val His Ser Gln 
                85                  90                  95      


Lys Ala Val Thr Asp Ala Gln Phe Leu Val Val Lys Glu Ala Leu Val 
            100                 105                 110         


Lys Thr Leu Lys Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Thr 
        115                 120                 125             


Thr Ala Val Glu Leu Ala Tyr Asp Glu Leu Ala Val Ala Ile Lys Lys 
    130                 135                 140                 


Ala Tyr 
145     


<210>  3
<211>  468
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA sequence of LlLegH

<400>  3
atgtctggtg ctttgactga atctcaagct gcattggtta agtcctcatg ggaagagttt       60

aatgctaaca tcccaaagca tacacataga tttttcattt tggttttaga aattgcacca      120

gctgcaaaag atttgttttc atttttgaag ggtacttcag aagttccaca aaataatcca      180

gaattgcaag ctcatgctgg taaagttttt aaattggttt acgaagctgc aattcaatta      240

gaagttacag gtgttgttgt tactgatgct acattgaaaa atttgggttc tgttcatgtt      300

tcaaaaggtg ttgctgatgc acattttcca gttgttaaag aagctatctt gaagactatt      360

aaagaagttg ttggtgcaaa atggtctgaa gaattaaatt ctgcttggac aatcgcatac      420

gatgaattag caatcgttat taagaaagaa atggatgatg ctgcataa                   468


<210>  4
<211>  155
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LlLegH

<400>  4

Met Ser Gly Ala Leu Thr Glu Ser Gln Ala Ala Leu Val Lys Ser Ser 
1               5                   10                  15      


Trp Glu Glu Phe Asn Ala Asn Ile Pro Lys His Thr His Arg Phe Phe 
            20                  25                  30          


Ile Leu Val Leu Glu Ile Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe 
        35                  40                  45              


Leu Lys Gly Thr Ser Glu Val Pro Gln Asn Asn Pro Glu Leu Gln Ala 
    50                  55                  60                  


His Ala Gly Lys Val Phe Lys Leu Val Tyr Glu Ala Ala Ile Gln Leu 
65                  70                  75                  80  


Glu Val Thr Gly Val Val Val Thr Asp Ala Thr Leu Lys Asn Leu Gly 
                85                  90                  95      


Ser Val His Val Ser Lys Gly Val Ala Asp Ala His Phe Pro Val Val 
            100                 105                 110         


Lys Glu Ala Ile Leu Lys Thr Ile Lys Glu Val Val Gly Ala Lys Trp 
        115                 120                 125             


Ser Glu Glu Leu Asn Ser Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala 
    130                 135                 140                 


Ile Val Ile Lys Lys Glu Met Asp Asp Ala Ala 
145                 150                 155 


<210>  5
<211>  468
<212>  DNA
<213>  Bos taurus

<400>  5
atgtctggtt tgtctgatgg tgaatggcaa ttggttttaa atgcttgggg taaagttgaa       60

gctgatgttg caggtcatgg tcaagaagtt ttgatcagat tgtttactgg tcatccagaa      120

acattggaaa agttcgataa gtttaaacat ttgaagactg aagctgaaat gaaagcatca      180

gaagatttga agaaacatgg taacactgtt ttgacagctt tgggtggtat tttgaaaaag      240

aaaggtcatc atgaagcaga agttaaacat ttggctgaat ctcatgcaaa taagcataag      300

atccctgtta agtatttgga gtttatttca gatgctatca tccatgtctt acatgcaaaa      360

catccatctg attttggtgc tgatgcacaa gctgcaatgt ctaaagcatt ggaattgttt      420

agaaacgata tggctgcaca atacaaagtt ttgggttttc atggttaa                   468


<210>  6
<211>  765
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA sequence of HLTev-VsLegH

<400>  6
atgcaccacc accaccacca cagcggcgct tttgaattta agctgccgga cattggcgaa       60

ggcatccacg aaggtgaaat tgtcaaatgg tttgtgaaac cgggcgatga agtgaacgaa      120

gacgatgtat tgtgcgaagt gcaaaatgac aaggcggttg tcgaaattcc ctccccggtc      180

aaagggaaag tgcttgaaat cctcgtcccg gagggaacag tggcaacggt cgggcaaacg      240

ctcatcacgc tcgatgcgcc gggttatgaa aacatgacga ccggcagcga caccggcgaa      300

aacctgtact tccagggtgg atccatgtct gttgcttttt ctgataagca agaagcattg      360

gttaatggtt catgggaagc ctttaaaact aacatcccaa agtactctgt tgttttctat      420

actacaattt tagaaaaagc tccagctgct aaaaatttgt tttcattttt ggcaaatggt      480

gttgatccat caaatccaaa attgttagct catgcagaaa aattgttcgg tttagttaga      540

gattctgctg cacaattgag agcttcaggt acagttgttg cagatgctgc attaggttct      600

gttcattcac aaaaagctgt tactgatgca caattcttgg ttgttaaaga agcattggtt      660

aagacattga aagaagcagt tggtgacaaa tggtcagatg aattaactac agctgttgaa      720

ttggcttatg atgaattagc tgttgctatt aaaaaggctt attaa                      765


<210>  7
<211>  254
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of HLTev-VsLegH

<400>  7

Met His His His His His His Ser Gly Ala Phe Glu Phe Lys Leu Pro 
1               5                   10                  15      


Asp Ile Gly Glu Gly Ile His Glu Gly Glu Ile Val Lys Trp Phe Val 
            20                  25                  30          


Lys Pro Gly Asp Glu Val Asn Glu Asp Asp Val Leu Cys Glu Val Gln 
        35                  40                  45              


Asn Asp Lys Ala Val Val Glu Ile Pro Ser Pro Val Lys Gly Lys Val 
    50                  55                  60                  


Leu Glu Ile Leu Val Pro Glu Gly Thr Val Ala Thr Val Gly Gln Thr 
65                  70                  75                  80  


Leu Ile Thr Leu Asp Ala Pro Gly Tyr Glu Asn Met Thr Thr Gly Ser 
                85                  90                  95      


Asp Thr Gly Glu Asn Leu Tyr Phe Gln Gly Gly Ser Met Ser Val Ala 
            100                 105                 110         


Phe Ser Asp Lys Gln Glu Ala Leu Val Asn Gly Ser Trp Glu Ala Phe 
        115                 120                 125             


Lys Thr Asn Ile Pro Lys Tyr Ser Val Val Phe Tyr Thr Thr Ile Leu 
    130                 135                 140                 


Glu Lys Ala Pro Ala Ala Lys Asn Leu Phe Ser Phe Leu Ala Asn Gly 
145                 150                 155                 160 


Val Asp Pro Ser Asn Pro Lys Leu Leu Ala His Ala Glu Lys Leu Phe 
                165                 170                 175     


Gly Leu Val Arg Asp Ser Ala Ala Gln Leu Arg Ala Ser Gly Thr Val 
            180                 185                 190         


Val Ala Asp Ala Ala Leu Gly Ser Val His Ser Gln Lys Ala Val Thr 
        195                 200                 205             


Asp Ala Gln Phe Leu Val Val Lys Glu Ala Leu Val Lys Thr Leu Lys 
    210                 215                 220                 


Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Thr Thr Ala Val Glu 
225                 230                 235                 240 


Leu Ala Tyr Asp Glu Leu Ala Val Ala Ile Lys Lys Ala Tyr 
                245                 250                 


<210>  8
<211>  792
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA sequence of HLTev-LlLegH

<400>  8
atgcaccacc accaccacca cagcggcgct tttgaattta agctgccgga cattggcgaa       60

ggcatccacg aaggtgaaat tgtcaaatgg tttgtgaaac cgggcgatga agtgaacgaa      120

gacgatgtat tgtgcgaagt gcaaaatgac aaggcggttg tcgaaattcc ctccccggtc      180

aaagggaaag tgcttgaaat cctcgtcccg gagggaacag tggcaacggt cgggcaaacg      240

ctcatcacgc tcgatgcgcc gggttatgaa aacatgacga ccggcagcga caccggcgaa      300

aacctgtact tccagggtgg atccatgtct ggtgctttga ctgaatctca agctgcattg      360

gttaagtcct catgggaaga gtttaatgct aacatcccaa agcatacaca tagatttttc      420

attttggttt tagaaattgc accagctgca aaagatttgt tttcattttt gaagggtact      480

tcagaagttc cacaaaataa tccagaattg caagctcatg ctggtaaagt ttttaaattg      540

gtttacgaag ctgcaattca attagaagtt acaggtgttg ttgttactga tgctacattg      600

aaaaatttgg gttctgttca tgtttcaaaa ggtgttgctg atgcacattt tccagttgtt      660

aaagaagcta tcttgaagac tattaaagaa gttgttggtg caaaatggtc tgaagaatta      720

aattctgctt ggacaatcgc atacgatgaa ttagcaatcg ttattaagaa agaaatggat      780

gatgctgcat aa                                                          792


<210>  9
<211>  263
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of HLTev-LlLegH

<400>  9

Met His His His His His His Ser Gly Ala Phe Glu Phe Lys Leu Pro 
1               5                   10                  15      


Asp Ile Gly Glu Gly Ile His Glu Gly Glu Ile Val Lys Trp Phe Val 
            20                  25                  30          


Lys Pro Gly Asp Glu Val Asn Glu Asp Asp Val Leu Cys Glu Val Gln 
        35                  40                  45              


Asn Asp Lys Ala Val Val Glu Ile Pro Ser Pro Val Lys Gly Lys Val 
    50                  55                  60                  


Leu Glu Ile Leu Val Pro Glu Gly Thr Val Ala Thr Val Gly Gln Thr 
65                  70                  75                  80  


Leu Ile Thr Leu Asp Ala Pro Gly Tyr Glu Asn Met Thr Thr Gly Ser 
                85                  90                  95      


Asp Thr Gly Glu Asn Leu Tyr Phe Gln Gly Gly Ser Met Ser Gly Ala 
            100                 105                 110         


Leu Thr Glu Ser Gln Ala Ala Leu Val Lys Ser Ser Trp Glu Glu Phe 
        115                 120                 125             


Asn Ala Asn Ile Pro Lys His Thr His Arg Phe Phe Ile Leu Val Leu 
    130                 135                 140                 


Glu Ile Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu Lys Gly Thr 
145                 150                 155                 160 


Ser Glu Val Pro Gln Asn Asn Pro Glu Leu Gln Ala His Ala Gly Lys 
                165                 170                 175     


Val Phe Lys Leu Val Tyr Glu Ala Ala Ile Gln Leu Glu Val Thr Gly 
            180                 185                 190         


Val Val Val Thr Asp Ala Thr Leu Lys Asn Leu Gly Ser Val His Val 
        195                 200                 205             


Ser Lys Gly Val Ala Asp Ala His Phe Pro Val Val Lys Glu Ala Ile 
    210                 215                 220                 


Leu Lys Thr Ile Lys Glu Val Val Gly Ala Lys Trp Ser Glu Glu Leu 
225                 230                 235                 240 


Asn Ser Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala Ile Val Ile Lys 
                245                 250                 255     


Lys Glu Met Asp Asp Ala Ala 
            260             


<210>  10
<211>  324
<212>  DNA
<213>  artificial sequence

<220>
<223>  DNA sequence of HLTev

<400>  10
atgcaccacc accaccacca cagcggcgct tttgaattta agctgccgga cattggcgaa       60

ggcatccacg aaggtgaaat tgtcaaatgg tttgtgaaac cgggcgatga agtgaacgaa      120

gacgatgtat tgtgcgaagt gcaaaatgac aaggcggttg tcgaaattcc ctccccggtc      180

aaagggaaag tgcttgaaat cctcgtcccg gagggaacag tggcaacggt cgggcaaacg      240

ctcatcacgc tcgatgcgcc gggttatgaa aacatgacga ccggcagcga caccggcgaa      300

aacctgtact tccagggtgg atcc                                             324


<210>  11
<211>  15
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of FBA-tag

<400>  11

Gly Val Glu Gln Ile Leu Lys Arg Lys Thr Gly Val Ile Val Gly 
1               5                   10                  15  


<210>  12
<211>  4
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of SKIK-tag

<400>  12

Ser Lys Ile Lys 
1               


<210>  13
<211>  43
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer SKIK-VsLegH-F

<400>  13
atgtctaaaa taaaatctgt tgctttttct gataagcaag aag                         43


<210>  14
<211>  76
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer FBA-VsLegH-F

<400>  14
atgggtgttg aacaaatctt aaagagaaag accggtgtca tcgttggttc tgttgctttt       60

tctgataagc aagaag                                                       76


<210>  15
<211>  37
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer SKIK-LlLegH-F

<400>  15
atgtctaaaa taaaatctgg tgctttgact gaatctc                                37


<210>  16
<211>  70
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer FBA-LlLegH F

<400>  16
atgggtgttg aacaaatctt aaagagaaag accggtgtca tcgttggttc tggtgctttg       60

actgaatctc                                                              70


<210>  17
<211>  38
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer SKIK-BtMyg-F

<400>  17
atgtctaaaa taaaatctgg tttgtctgat ggtgaatg                               38


<210>  18
<211>  71
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer FBA-BtMyg-F

<400>  18
atgggtgttg aacaaatctt aaagagaaag accggtgtca tcgttggttc tggtttgtct       60

gatggtgaat g                                                            71


<210>  19
<211>  33
<212>  DNA
<213>  artificial sequence

<220>
<223>  primer pYES2-R

<400>  19
ttttttaagc ttaatattcc ctatagtgag tcg                                    33


<210>  20
<211>  155
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of BtMyg

<400>  20

Met Ser Gly Leu Ser Asp Gly Glu Trp Gln Leu Val Leu Asn Ala Trp 
1               5                   10                  15      


Gly Lys Val Glu Ala Asp Val Ala Gly His Gly Gln Glu Val Leu Ile 
            20                  25                  30          


Arg Leu Phe Thr Gly His Pro Glu Thr Leu Glu Lys Phe Asp Lys Phe 
        35                  40                  45              


Lys His Leu Lys Thr Glu Ala Glu Met Lys Ala Ser Glu Asp Leu Lys 
    50                  55                  60                  


Lys His Gly Asn Thr Val Leu Thr Ala Leu Gly Gly Ile Leu Lys Lys 
65                  70                  75                  80  


Lys Gly His His Glu Ala Glu Val Lys His Leu Ala Glu Ser His Ala 
                85                  90                  95      


Asn Lys His Lys Ile Pro Val Lys Tyr Leu Glu Phe Ile Ser Asp Ala 
            100                 105                 110         


Ile Ile His Val Leu His Ala Lys His Pro Ser Asp Phe Gly Ala Asp 
        115                 120                 125             


Ala Gln Ala Ala Met Ser Lys Ala Leu Glu Leu Phe Arg Asn Asp Met 
    130                 135                 140                 


Ala Ala Gln Tyr Lys Val Leu Gly Phe His Gly 
145                 150                 155 


<210>  21
<211>  161
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of FBA-VsLegH

<400>  21

Met Gly Val Glu Gln Ile Leu Lys Arg Lys Thr Gly Val Ile Val Gly 
1               5                   10                  15      


Ser Val Ala Phe Ser Asp Lys Gln Glu Ala Leu Val Asn Gly Ser Trp 
            20                  25                  30          


Glu Ala Phe Lys Thr Asn Ile Pro Lys Tyr Ser Val Val Phe Tyr Thr 
        35                  40                  45              


Thr Ile Leu Glu Lys Ala Pro Ala Ala Lys Asn Leu Phe Ser Phe Leu 
    50                  55                  60                  


Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu Leu Ala His Ala Glu 
65                  70                  75                  80  


Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala Gln Leu Arg Ala Ser 
                85                  90                  95      


Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Val His Ser Gln Lys 
            100                 105                 110         


Ala Val Thr Asp Ala Gln Phe Leu Val Val Lys Glu Ala Leu Val Lys 
        115                 120                 125             


Thr Leu Lys Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Thr Thr 
    130                 135                 140                 


Ala Val Glu Leu Ala Tyr Asp Glu Leu Ala Val Ala Ile Lys Lys Ala 
145                 150                 155                 160 


Tyr 
    


<210>  22
<211>  150
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of SKIK-VsLegH

<400>  22

Met Ser Lys Ile Lys Ser Val Ala Phe Ser Asp Lys Gln Glu Ala Leu 
1               5                   10                  15      


Val Asn Gly Ser Trp Glu Ala Phe Lys Thr Asn Ile Pro Lys Tyr Ser 
            20                  25                  30          


Val Val Phe Tyr Thr Thr Ile Leu Glu Lys Ala Pro Ala Ala Lys Asn 
        35                  40                  45              


Leu Phe Ser Phe Leu Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu 
    50                  55                  60                  


Leu Ala His Ala Glu Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala 
65                  70                  75                  80  


Gln Leu Arg Ala Ser Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser 
                85                  90                  95      


Val His Ser Gln Lys Ala Val Thr Asp Ala Gln Phe Leu Val Val Lys 
            100                 105                 110         


Glu Ala Leu Val Lys Thr Leu Lys Glu Ala Val Gly Asp Lys Trp Ser 
        115                 120                 125             


Asp Glu Leu Thr Thr Ala Val Glu Leu Ala Tyr Asp Glu Leu Ala Val 
    130                 135                 140                 


Ala Ile Lys Lys Ala Tyr 
145                 150 


<210>  23
<211>  154
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LaLegH1

<400>  23

Met Ala Ile Leu Thr Asp Val Gln Val Ala Leu Val Lys Ser Ser Phe 
1               5                   10                  15      


Glu Glu Phe Asn Ala Asn Ile Pro Lys His Thr His Arg Phe Phe Thr 
            20                  25                  30          


Leu Val Leu Glu Ile Ala Pro Thr Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Lys Gly Thr Asn Glu Val Pro Gln Asn Asn Pro Asp Leu Gln Ala His 
    50                  55                  60                  


Ala Gly Lys Val Phe Lys Leu Thr Tyr Glu Ala Ala Ile Gln Leu Gln 
65                  70                  75                  80  


Val Asn Gly Ala Val Ala Thr Asp Ala Thr Leu Lys Asn Leu Gly Ser 
                85                  90                  95      


Val His Val Ser Lys Gly Val Ile Asp Ala His Phe Pro Val Val Lys 
            100                 105                 110         


Glu Ala Ile Leu Lys Thr Ile Lys Glu Val Val Gly Asp Lys Trp Ser 
        115                 120                 125             


Glu Glu Leu Asn Asn Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala Ile 
    130                 135                 140                 


Ile Ile Lys Lys Glu Met Lys Asp Ala Ala 
145                 150                 


<210>  24
<211>  149
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LaLegH2

<400>  24

Met Gly Ala Leu Thr Glu Ser Gln Ala Ala Leu Val Lys Ser Ser Phe 
1               5                   10                  15      


Glu Glu Phe Asn Ala Asn Ile Pro Lys His Thr Gln Arg Phe Phe Ile 
            20                  25                  30          


Leu Val Leu Glu Ile Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Lys Gly Thr Ser Glu Val Pro Gln Asn Asn Pro Glu Leu Gln Ala His 
    50                  55                  60                  


Ala Gly Lys Val Phe Lys Leu Val Tyr Glu Ala Ala Ile Gln Leu Gln 
65                  70                  75                  80  


Val Thr Gly Ala Val Val Ala Asp Ala Thr Leu Lys Asn Leu Gly Ser 
                85                  90                  95      


Ile His Val Ser Lys Gly Val Val Asp Ala His Phe Pro Val Val Lys 
            100                 105                 110         


Glu Ala Ile Leu Lys Thr Ile Lys Glu Val Val Gly Asp Lys Trp Ser 
        115                 120                 125             


Glu Glu Leu Gly Asn Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala Ile 
    130                 135                 140                 


Val Ile Lys Arg Gly 
145                 


<210>  25
<211>  154
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LlLegH1

<400>  25

Met Gly Val Leu Thr Asp Val Gln Val Ala Leu Val Lys Ser Ser Phe 
1               5                   10                  15      


Glu Glu Phe Asn Ala Asn Ile Pro Lys Asn Thr His Arg Phe Phe Thr 
            20                  25                  30          


Leu Val Leu Glu Ile Ala Pro Gly Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Lys Gly Ser Ser Glu Val Pro Gln Asn Asn Pro Asp Leu Gln Ala His 
    50                  55                  60                  


Ala Gly Lys Val Phe Lys Leu Thr Tyr Glu Ala Ala Ile Gln Leu Gln 
65                  70                  75                  80  


Val Asn Gly Ala Val Ala Ser Asp Ala Thr Leu Lys Ser Leu Gly Ser 
                85                  90                  95      


Val His Val Ser Lys Gly Val Val Asp Ala His Phe Pro Val Val Lys 
            100                 105                 110         


Glu Ala Ile Leu Lys Thr Ile Lys Glu Val Val Gly Asp Lys Trp Ser 
        115                 120                 125             


Glu Glu Leu Asn Thr Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala Ile 
    130                 135                 140                 


Ile Ile Lys Lys Glu Met Lys Asp Ala Ala 
145                 150                 


<210>  26
<211>  148
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LalLegH

<400>  26

Met Gly Ala Leu Thr Glu Thr Gln Ala Ala Leu Val Lys Ser Ser Trp 
1               5                   10                  15      


Glu Glu Phe Asn Ala Asn Ile Pro Lys His Thr His Leu Phe Phe Thr 
            20                  25                  30          


Leu Val Leu Glu Ile Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Lys Gly Thr Ser Glu Val Pro Leu Asn Ser Pro Glu Leu Gln Ala His 
    50                  55                  60                  


Ala Gly Lys Val Phe Lys Leu Val Tyr Glu Ala Ala Ile Gln Leu Gln 
65                  70                  75                  80  


Val Asn Gly Val Ile Val Thr Asp Ala Thr Ser Lys Asn Leu Gly Ser 
                85                  90                  95      


Leu His Val Ser Lys Gly Val Val Asp Ala His Phe Pro Val Val Lys 
            100                 105                 110         


Glu Ala Ile Leu Lys Thr Ile Lys Glu Val Val Gly Ala Lys Trp Ser 
        115                 120                 125             


Glu Glu Leu Ser Thr Ala Trp Thr Ile Ala Tyr Asp Glu Leu Ala Ile 
    130                 135                 140                 


Val Ile Lys Asn 
145             


<210>  27
<211>  462
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LaLegH1

<400>  27
atggcgattt tgacggatgt tcaggttgcc ctcgttaagt cctcgtttga ggagtttaac       60

gccaacattc cgaaacacac acatcgcttc tttacgctgg tcttggaaat tgctcctact      120

gccaaagacc tgtttagctt cctgaaaggc acgaatgaag tgccgcaaaa caacccggat      180

ttacaggcac atgctgggaa agtgttcaaa ctgacctatg aagcagcgat tcagcttcaa      240

gtgaatggag ctgttgcgac tgatgcaacc ctgaagaatc tgggttcagt ccatgtctct      300

aaaggtgtga tcgatgcaca ctttccagtg gtgaaagaag cgattctgaa aaccatcaaa      360

gaagtagtag gcgacaaatg gagtgaagaa ctgaacaatg cgtggaccat tgcctacgac      420

gaattagcga tcatcatcaa gaaggagatg aaagatgcgg cc                         462


<210>  28
<211>  462
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LlLegH1

<400>  28
atgggcgttc tgaccgatgt ccaagttgct ctggtgaaat cgagctttga ggagtttaac       60

gcgaacattc cgaagaatac ccatcgcttc ttcacgttag tgctggaaat tgcaccaggt      120

gctaaagatc tgttttcctt cctcaaaggg agttctgaag taccgcagaa taacccggac      180

ttacaagcgc atgcaggcaa agtctttaag ttgacgtatg aagccgctat tcagcttcag      240

gtgaatggtg cagtagcctc agatgcgaca ctgaaaagcc tgggtagtgt gcacgtgtcg      300

aaaggcgtcg ttgatgcgca ctttcctgtg gttaaagaag cgattctgaa aaccatcaag      360

gaagtcgtag gagacaaatg gagcgaagaa ctgaacactg catggaccat tgcctacgac      420

gaattggcca tcatcatcaa gaaagagatg aaagatgcgg cc                         462


<210>  29
<211>  765
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of HLTev-VsLegH

<400>  29
atgcaccacc accaccacca cagcggcgct tttgaattta agctgccgga cattggcgaa       60

ggcatccacg aaggtgaaat tgtcaaatgg tttgtgaaac cgggcgatga agtgaacgaa      120

gacgatgtat tgtgcgaagt gcaaaatgac aaggcggttg tcgaaattcc ctccccggtc      180

aaagggaaag tgcttgaaat cctcgtcccg gagggaacag tggcaacggt cgggcaaacg      240

ctcatcacgc tcgatgcgcc gggttatgaa aacatgacga ccggcagcga caccggcgaa      300

aacctgtact tccagggtgg atccatgtct gttgcttttt ctgataagca agaagcattg      360

gttaatggtt catgggaagc ctttaaaact aacatcccaa agtactctgt tgttttctat      420

actacaattt tagaaaaagc tccagctgct aaaaatttgt tttcattttt ggcaaatggt      480

gttgatccat caaatccaaa attgttagct catgcagaaa aattgttcgg tttagttaga      540

gattctgctg cacaattgag agcttcaggt acagttgttg cagatgctgc attaggttct      600

gttcattcac aaaaagctgt tactgatgca caattcttgg ttgttaaaga agcattggtt      660

aagacattga aagaagcagt tggtgacaaa tggtcagatg aattaactac agctgttgaa      720

ttggcttatg atgaattagc tgttgctatt aaaaaggctt attaa                      765


<210>  30
<211>  792
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of HLTev-LlLegH

<400>  30
atgcaccacc accaccacca cagcggcgct tttgaattta agctgccgga cattggcgaa       60

ggcatccacg aaggtgaaat tgtcaaatgg tttgtgaaac cgggcgatga agtgaacgaa      120

gacgatgtat tgtgcgaagt gcaaaatgac aaggcggttg tcgaaattcc ctccccggtc      180

aaagggaaag tgcttgaaat cctcgtcccg gagggaacag tggcaacggt cgggcaaacg      240

ctcatcacgc tcgatgcgcc gggttatgaa aacatgacga ccggcagcga caccggcgaa      300

aacctgtact tccagggtgg atccatgtct ggtgctttga ctgaatctca agctgcattg      360

gttaagtcct catgggaaga gtttaatgct aacatcccaa agcatacaca tagatttttc      420

attttggttt tagaaattgc accagctgca aaagatttgt tttcattttt gaagggtact      480

tcagaagttc cacaaaataa tccagaattg caagctcatg ctggtaaagt ttttaaattg      540

gtttacgaag ctgcaattca attagaagtt acaggtgttg ttgttactga tgctacattg      600

aaaaatttgg gttctgttca tgtttcaaaa ggtgttgctg atgcacattt tccagttgtt      660

aaagaagcta tcttgaagac tattaaagaa gttgttggtg caaaatggtc tgaagaatta      720

aattctgctt ggacaatcgc atacgatgaa ttagcaatcg ttattaagaa agaaatggat      780

gatgctgcat aa                                                          792


<210>  31
<211>  144
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LegH C1

<400>  31

Met Gly Ala Phe Thr Glu Lys Gln Glu Ala Leu Val Ser Ser Ser Phe 
1               5                   10                  15      


Glu Ala Phe Lys Ala Asn Ile Pro Gln Tyr Ser Val Val Phe Tyr Asn 
            20                  25                  30          


Ser Ile Leu Glu Lys Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Ala Asn Gly Val Asp Pro Thr Asn Pro Lys Leu Thr Gly His Ala Glu 
    50                  55                  60                  


Lys Leu Phe Ala Leu Val Arg Asp Ser Ala Gly Gln Leu Lys Thr Asn 
65                  70                  75                  80  


Gly Thr Val Val Ala Asp Ala Ala Leu Val Ser Ile His Ala Gln Lys 
                85                  90                  95      


Ala Val Thr Asp Pro Gln Phe Val Val Val Lys Glu Ala Leu Leu Lys 
            100                 105                 110         


Thr Ile Lys Glu Ala Val Gly Gly Asn Trp Ser Asp Glu Leu Ser Ser 
        115                 120                 125             


Ala Trp Glu Val Ala Tyr Asp Glu Leu Ala Ala Ala Ile Lys Lys Ala 
    130                 135                 140                 


<210>  32
<211>  145
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LegH C2

<400>  32

Met Gly Ala Phe Thr Glu Lys Gln Glu Ala Leu Val Ser Ser Ser Phe 
1               5                   10                  15      


Glu Ala Phe Lys Ala Asn Ile Pro Gln Tyr Ser Val Val Phe Tyr Thr 
            20                  25                  30          


Ser Ile Leu Glu Lys Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Ser Asn Gly Val Asp Pro Ser Asn Pro Lys Leu Thr Gly His Ala Glu 
    50                  55                  60                  


Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Gly Gln Leu Lys Ala Asn 
65                  70                  75                  80  


Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Ile His Ala Gln Lys 
                85                  90                  95      


Ala Ile Thr Asp Pro Gln Phe Val Val Val Lys Glu Ala Leu Leu Lys 
            100                 105                 110         


Thr Ile Lys Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Ser Ser 
        115                 120                 125             


Ala Trp Glu Val Ala Tyr Asp Glu Leu Ala Ala Ala Ile Lys Lys Ala 
    130                 135                 140                 


Phe 
145 


<210>  33
<211>  145
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LegH C3

<400>  33

Met Gly Ala Phe Thr Asp Lys Gln Glu Ala Leu Val Ser Ser Ser Phe 
1               5                   10                  15      


Glu Ala Phe Lys Thr Asn Ile Pro Gln Tyr Ser Val Val Phe Tyr Thr 
            20                  25                  30          


Ser Ile Leu Glu Lys Ala Pro Val Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Ala Asn Gly Val Asp Pro Thr Asn Pro Lys Leu Thr Gly His Ala Glu 
    50                  55                  60                  


Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Gly Gln Leu Lys Ala Ser 
65                  70                  75                  80  


Gly Thr Val Val Ile Asp Ala Ala Leu Gly Ser Ile His Gly Gln Lys 
                85                  90                  95      


Ala Ile Thr Asp Pro Gln Phe Val Val Val Lys Glu Ala Leu Leu Lys 
            100                 105                 110         


Thr Ile Lys Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Ser Ser 
        115                 120                 125             


Ala Trp Glu Val Ala Tyr Asp Glu Leu Ala Ala Ala Ile Lys Lys Ala 
    130                 135                 140                 


Phe 
145 


<210>  34
<211>  144
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of LegH A

<400>  34

Met Val Ala Phe Thr Glu Lys Gln Asp Ala Leu Val Ser Ser Ser Phe 
1               5                   10                  15      


Glu Ala Phe Lys Ala Asn Ile Pro Gln Tyr Ser Val Val Phe Tyr Thr 
            20                  25                  30          


Ser Ile Leu Glu Lys Ala Pro Ala Ala Lys Asp Leu Phe Ser Phe Leu 
        35                  40                  45              


Ala Asn Gly Val Asp Pro Thr Asn Pro Lys Leu Thr Gly His Ala Glu 
    50                  55                  60                  


Lys Leu Phe Ala Leu Val Arg Asp Ser Ala Gly Gln Leu Lys Ala Ser 
65                  70                  75                  80  


Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Val His Ala Gln Lys 
                85                  90                  95      


Ala Val Thr Asp Pro Gln Phe Val Val Val Lys Glu Ala Leu Leu Lys 
            100                 105                 110         


Thr Ile Lys Ala Ala Val Gly Asp Lys Trp Ser Asp Glu Leu Ser Arg 
        115                 120                 125             


Ala Trp Glu Val Ala Tyr Asp Glu Leu Ala Ala Ala Ile Lys Lys Ala 
    130                 135                 140                 


<210>  35
<211>  115
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of VsLegH9

<400>  35

Met Asn Arg Ile Leu Glu Lys Ala Pro Ala Ala Lys Asn Leu Phe Ser 
1               5                   10                  15      


Phe Leu Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu Leu Ala His 
            20                  25                  30          


Ala Glu Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala Gln Leu Arg 
        35                  40                  45              


Ala Ser Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Val His Ser 
    50                  55                  60                  


Gln Lys Ala Val Thr Asp Ala Gln Phe Leu Val Val Lys Glu Ala Leu 
65                  70                  75                  80  


Val Lys Thr Leu Lys Glu Ala Val Gly Asp Lys Trp Ser Asp Glu Leu 
                85                  90                  95      


Ala Thr Ala Val Glu Leu Ala Tyr Asp Glu Leu Ala Val Ala Ile Lys 
            100                 105                 110         


Lys Ala Tyr 
        115 


<210>  36
<211>  267
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of VsLegH10

<400>  36

Met Val His Gly Lys His Ser Arg Gln Thr Phe Leu Asn Thr Val Leu 
1               5                   10                  15      


Ser Ser Thr Pro Arg Glu Ile Leu Glu Lys Ala Pro Ala Ala Lys Asn 
            20                  25                  30          


Leu Phe Ser Phe Leu Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu 
        35                  40                  45              


Leu Ala His Ala Glu Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala 
    50                  55                  60                  


Gln Leu Arg Ala Ser Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser 
65                  70                  75                  80  


Val His Ser Gln Lys Ala Val Thr Asp Ala Gln Phe Leu Val Val Lys 
                85                  90                  95      


Glu Ala Leu Val Lys Thr Leu Lys Glu Ala Val Gly Asp Lys Trp Ser 
            100                 105                 110         


Asp Glu Leu Ala Thr Ala Val Glu Leu Ala Tyr Asp Glu Leu Ala Lys 
        115                 120                 125             


Gln Glu Gln Leu Val Asn Ser Ser Trp Glu Ala Phe Lys Gly Asn Leu 
    130                 135                 140                 


Ser His Asn Ser Ala Thr Phe Phe Asn Leu Val Leu Glu Lys Glu Pro 
145                 150                 155                 160 


Gly Ala Lys Asn Leu Phe Ser Phe Leu Gly Asp Gly Val Asp Pro Asn 
                165                 170                 175     


Asn Pro Lys Ile Ala Ala His Ala Glu Lys Leu Phe Gly Thr Val Arg 
            180                 185                 190         


Asp Ala Ala Val Gln Leu Lys Thr Lys Gly Glu Val Val Ala Glu Ala 
        195                 200                 205             


Lys Leu Gly Ser Val His Ala Glu Lys Lys Val Thr Asp Pro Gln Phe 
    210                 215                 220                 


Ala Val Ile Lys Glu Ala Val Leu Lys Thr Ile Lys Glu Ala Val Gly 
225                 230                 235                 240 


Glu Lys Trp Asn Glu Glu Val Asn Asp Ala Trp Ala Gln Ala Tyr Asp 
                245                 250                 255     


Ala Met Ala Ala Ala Ile Lys Lys Thr Ile Ala 
            260                 265         


<210>  37
<211>  83
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of VsLegH61

<400>  37

Met Asn Arg Ile Leu Glu Lys Ala Pro Ile Ala Lys Asn Leu Phe Ser 
1               5                   10                  15      


Phe Leu Ala Asn Gly Val Asp Pro Ser Asn Pro Lys Leu Val Ala His 
            20                  25                  30          


Ala Glu Lys Leu Phe Gly Leu Val Arg Asp Ser Ala Ala Gln Leu Arg 
        35                  40                  45              


Ala Ser Gly Thr Val Val Ala Asp Ala Ala Leu Gly Ser Val His Ser 
    50                  55                  60                  


Gln Lys Ala Val Thr Asp Ala Gln Tyr Leu Ile Pro Leu Val Thr Ile 
65                  70                  75                  80  


Val Leu Trp 
            


<210>  38
<211>  146
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence of bact. hemoglobin from Vitreoscilla 
       stercoraria

<400>  38

Met Leu Asp Gln Gln Thr Ile Asn Ile Ile Lys Ala Thr Val Pro Val 
1               5                   10                  15      


Leu Lys Glu His Gly Val Thr Ile Thr Thr Thr Phe Tyr Lys Asn Leu 
            20                  25                  30          


Phe Ala Lys His Pro Glu Val Arg Pro Leu Phe Asp Met Gly Arg Gln 
        35                  40                  45              


Glu Ser Leu Glu Gln Pro Lys Ala Leu Ala Met Thr Val Leu Ala Ala 
    50                  55                  60                  


Ala Gln Asn Ile Glu Asn Leu Pro Ala Ile Leu Pro Ala Val Lys Lys 
65                  70                  75                  80  


Ile Ala Val Lys His Cys Gln Ala Gly Val Ala Ala Ala His Tyr Pro 
                85                  90                  95      


Ile Val Gly Gln Glu Leu Leu Gly Ala Ile Lys Glu Val Leu Gly Asp 
            100                 105                 110         


Ala Ala Thr Asp Asp Ile Leu Asp Ala Trp Gly Lys Ala Tyr Gly Val 
        115                 120                 125             


Ile Ala Asp Val Phe Ile Gln Val Glu Ala Asp Leu Tyr Ala Gln Ala 
    130                 135                 140                 


Val Glu 
145     


<210>  39
<211>  432
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LegH C1

<400>  39
atgggcgcct ttaccgagaa acaagaagcg ctggtgtcaa gcagcttcga agcgtttaaa       60

gccaacattc cgcagtattc cgtggtgttc tacaactcga ttctggaaaa ggcccctgct      120

gccaaagacc tgttcagctt tctggccaat ggcgtagatc caaccaaccc gaaactcacc      180

ggtcatgcgg aaaagctgtt tgcgttggtt cgcgatagtg ctggtcagct gaaaacgaat      240

ggcacagttg tggctgatgc agccttagtc tccattcacg cacagaaagc ggtcactgat      300

ccgcaatttg tcgttgtgaa agaagcgttg ctgaaaacga tcaaagaggc agtaggaggg      360

aattggagtg acgaactttc gtctgcatgg gaagttgcgt atgacgagtt agctgcagcg      420

atcaagaaag cg                                                          432


<210>  40
<211>  435
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LegH C2

<400>  40
atgggagcgt ttacggagaa acaggaagca ctggttagtt ccagctttga ggccttcaaa       60

gccaacattc cgcaatattc cgtggtgttc tacaccagca ttctggagaa agcgcctgca      120

gccaaagacc tgttttcgtt cctctctaat ggcgttgatc cgtcaaaccc gaaattaacc      180

ggtcatgcgg aaaagctgtt tgggttggta cgcgatagtg cgggtcaact gaaggcgaat      240

ggcacagtcg tcgctgatgc agccttaggt agcattcacg cgcagaaagc catcactgat      300

ccacagtttg tggtggtcaa agaagcgctg ttgaaaacca tcaaagaagc tgttggcgac      360

aaatggagcg atgaactgtc gtctgcatgg gaagtagcct atgacgaact tgcagctgct      420

atcaagaaag cgttc                                                       435


<210>  41
<211>  435
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LegH C3

<400>  41
atgggtgcct tcacagacaa acaggaagcc ttggtttcct ctagctttga ggctttcaag       60

accaacattc cgcagtattc ggtcgtgttc tacacctcca tcctggagaa agctccagtc      120

gccaaagacc tgttttcgtt cttagcaaac ggcgttgatc cgacgaatcc gaaactgact      180

ggacatgcgg agaaactgtt tggcttggtt cgcgattctg ccggtcaact caaagcgagt      240

ggtaccgtgg tcattgatgc ggcgttaggc agcattcacg ggcaaaaggc cattaccgat      300

cctcagtttg tggtggtgaa agaagcactg cttaaaacga tcaaagaagc tgtaggcgac      360

aaatggagtg atgaactgag ctcagcgtgg gaagtagcgt atgatgaact ggcagcagcc      420

atcaagaaag cgttt                                                       435


<210>  42
<211>  432
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of LegH A

<400>  42
atggttgcgt ttacggagaa acaggatgct ctcgttagct cgagcttcga agcctttaaa       60

gcgaacattc cgcagtattc ggtggtgttc tacacctcca ttctggagaa agctccagct      120

gcgaaagact tgttctcctt tctggccaat ggggtcgatc ctaccaaccc gaaattgaca      180

ggacacgcag aaaagctgtt tgcgttagta cgcgatagtg ccggtcaact gaaagcctca      240

ggtaccgttg tcgccgatgc tgcattaggc agtgttcatg cgcagaaagc ggtgactgat      300

ccgcaatttg tggtggtgaa agaagccctg cttaagacga tcaaagcagc ggtaggcgac      360

aaatggagcg atgaactgtc tcgtgcatgg gaagtcgcgt atgacgaact ggcagcagcg      420

atcaagaaag cc                                                          432


<210>  43
<211>  348
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of VsLegH9

<400>  43
atgaaccgca ttctggagaa agccccagca gcgaagaacc ttttcagctt tctggccaat       60

ggtgtcgatc cgtcgaatcc gaaactcctg gcacatgcag agaagctgtt cgggttagtg      120

cgcgatagtg ctgcccaact gcgtgcttca ggcaccgttg ttgccgatgc agcgttaggc      180

tctgtacact cccagaaagc ggttaccgat gcgcagtttc tcgtcgtcaa agaagcgctg      240

gtgaaaacgc tgaaagaagc tgtgggtgac aaatggagcg acgaattggc aactgcggtg      300

gaactggcgt atgacgaatt ggccgtagcg atcaagaaag cctactaa                   348


<210>  44
<211>  801
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of VsLegH10

<400>  44
atggtacacg gcaaacacag ccgtcaaacg ttcctgaata ccgtgcttag ctcaactcct       60

cgcgagattc tcgaaaaagc cccagcagct aaaaacctgt ttagctttct ggcgaatggc      120

gttgatccga gtaatccgaa actgttagca catgctgaaa agctgtttgg gctggttcgc      180

gactcagcgg cgcaattacg cgcatctggc acggtagtgg ctgatgccgc attggggagt      240

gtccattccc agaaagccgt gaccgatgcg cagtttctgg ttgtgaagga agcactggtg      300

aaaacgctga aagaagccgt tggtgacaaa tggtcggatg aactggcaac agcggttgaa      360

ctcgcctatg acgagttagc gaaacaggaa cagctggtga atagctcttg ggaagcgttc      420

aaaggcaact tgtcgcacaa tagcgcgacc ttcttcaacc ttgtcctgga gaaagaacca      480

ggtgcgaaaa acctgttctc ctttctggga gatggagtcg atcccaacaa cccgaaaatt      540

gcggcacatg ccgagaaact ctttggcact gtacgtgatg ccgctgttca gttaaagacc      600

aaaggtgagg tagtggcgga agccaaactt ggttcggtgc atgcggagaa gaaagtcaca      660

gatccgcagt ttgcggtgat caaagaagcg gtgttgaaaa ccatcaagga agccgtcggt      720

gaaaaatgga acgaagaggt taatgacgcg tgggctcaag cctacgacgc aatggcagct      780

gctatcaaaa agaccattgc c                                                801


<210>  45
<211>  249
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of VsLegH61

<400>  45
atgaatcgca tccttgagaa agctccgatt gcgaagaacc tgttctcctt tctggccaat       60

ggtgtggacc cttcgaaccc gaaactggtc gcacatgccg aaaagctgtt tggccttgtt      120

cgcgactcag ctgctcaact ccgtgcttct ggtactgtag tggctgatgc ggcattaggc      180

tcagtacaca gccagaaagc cgttactgat gcgcagtacc tgatcccact ggtgaccatc      240

gtcttgtgg                                                              249


<210>  46
<211>  438
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid of bact. hemoglobin from Vitreoscilla stercoraria

<400>  46
atgctggatc agcagaccat taacatcatc aaagccactg ttccggtact gaaagaacac       60

ggcgttacca ttacgaccac cttctacaag aatctgtttg cgaaacatcc ggaagttcgt      120

cccctctttg acatgggtcg ccaagaaagc ttggaacagc cgaaagctct ggcgatgaca      180

gtgttagctg cagcccagaa catcgagaat ctgccagcga ttcttcctgc ggtgaagaaa      240

attgccgtga aacactgcca agcgggtgta gctgcggcac attacccgat tgttggccag      300

gaactgttag gtgccatcaa agaggtcttg ggcgatgcag ctacggacga tatcctggat      360

gcctggggaa aagcgtatgg ggtcattgcc gatgtgttca ttcaggtgga agcagacctg      420

tatgcacaag cggtcgag                                                    438


<210>  47
<211>  7010
<212>  DNA
<213>  artificial sequence

<220>
<223>  nucleic acid sequence of pTTB2-HLTev-VsLegH

<400>  47
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ccttaaggaa cgtacagacg      420

gcttaaaagc ctttaaaaac gtttttaagg ggtttgtaga caaggtaaag gataaaacag      480

cacaattcca agaaaaacac gatttagaac ctaaaaagaa cgaatttgaa ctaactcata      540

accgagaggt aaaaaaagaa cgaagtcgag atcagggaat gagtttataa aataaaaaaa      600

gcacctgaaa aggtgtcttt ttttgatggt tttgaacttg ttctttctta tcttgataca      660

tatagaaata acgtcatttt tattttagtt gctgaaaggt gcgttgaagt gttggtatgt      720

atgtgtttta aagtattgaa aacccttaaa attggttgca cagaaaaacc ccatctgtta      780

aagttataag tgactaaaca aataactaaa tagatggggg tttcttttaa tattatgtgt      840

cctaatagta gcatttattc agatgaaaaa tcaagggttt tagtggacaa gacaaaaagt      900

ggaaaagtga gaccatggag agaaaagaaa atcgctaatg ttgattactt tgaacttctg      960

catattcttg aatttaaaaa ggctgaaaga gtaaaagatt gtgctgaaat attagagtat     1020

aaacaaaatc gtgaaacagg cgaaagaaag ttgtatcgag tgtggttttg taaatccagg     1080

ctttgtccaa tgtgcaactg gaggagagca atgaaacatg gcattcagtc acaaaaggtt     1140

gttgctgaag ttattaaaca aaagccaaca gttcgttggt tgtttctcac attaacagtt     1200

aaaaatgttt atgatggcga agaattaaat aagagtttgt cagatatggc tcaaggattt     1260

cgccgaatga tgcaatataa aaaaattaat aaaaatcttg ttggttttat gcgtgcaacg     1320

gaagtgacaa taaataataa agataattct tataatcagc acatgcatgt attggtatgt     1380

gtggaaccaa cttattttaa gaatacagaa aactacgtga atcaaaaaca atggattcaa     1440

ttttggaaaa aggcaatgaa attagactat gatccaaatg taaaagttca aatgattcga     1500

ccgaaaaata aatataaatc ggatatacaa tcggcaattg acgaaactgc aaaatatcct     1560

gtaaaggata cggattttat gaccgatgat gaagaaaaga atttgaaacg tttgtctgat     1620

ttggaggaag gtttacaccg taaaaggtta atctcctatg gtggtttgtt aaaagaaata     1680

cataaaaaat taaaccttga tgacacagaa gaaggcgatt tgattcatac agatgatgac     1740

gaaaaagccg atgaagatgg attttctatt attgcaatgt ggaattggga acggaaaaat     1800

tattttatta aagagtagtt caacaaacgg gccagtttgt tgaagattag atgctataat     1860

tgttattaaa aggattgaag gatgcttagg aagacgagtt attaatagct gaataagaac     1920

ggtgctctcc aaatattctt atttagaaaa gcaaatctaa aattatctga aaagggaatg     1980

agaatattgt ctgaatccag cgcaagaacc gaaatgaaaa tcagcttgcc cgaaaaccta     2040

gtagctgaat tggatggtgt agcgatgcgg gagaaacgaa gcagaaacga actgatatca     2100

caagcagtga gagcgtatgt cagcgaacga acaactcgtc acaaccgtga tttgatgaga     2160

cgcggctata tggaaatggc gaaaatcaac ctgaatattt cttctgaggc tcactttgcg     2220

gagtgcgagg ctgaaacgac agttgagcgc ttagtcagcg gaggataaac gatgacctct     2280

aataattgtt aatcatgttg gttacgtatt tattaacttc tcctagtatt agtaattatc     2340

atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta     2400

ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt     2460

agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatcccggc attgccagtc     2520

ggggatatta aaaagagtat aggtttttat tgggataaag taggtttcac tttggttcac     2580

catgaagatg gattcgcagt tctaatgtgt aatgaggttc ggattcatct atgggaggca     2640

agtgatgaag gctggcgcct cgtagtaatg attcaccggt ttgtacaggt gcggagtcgt     2700

ttattgctgg tactgctagt tgccgcattg aagtagaggg aattgatgaa ttatatcaac     2760

atattaagcc tttgggcatt ttgcacccca atacatcatt aaaagatcag tggtgggatg     2820

aacgagactt tgcagtaatt gatcccgaca acaatttgat tagctttttt caacaaataa     2880

aaagctaaaa tctattatta atctgttcag caatcgggcg cgattgctga ataaaagata     2940

cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac actagaaaac     3000

cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct agacaaaacg     3060

gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat cttctcaaaa     3120

aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc ccctttgctg     3180

aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga aaggtcttaa     3240

aggttttatg gttttggtcg gcactgccgc gcctcgcaga gcacacactt tatgaatata     3300

aagtatagtg tgttatactt tacttggaag tggttgccgg aaagagcgaa aatgcctcac     3360

atttgtgcca cctaaaaagg agcgatttac atatgagtta tgcagtttgt agaatgcaaa     3420

aagtgaaatc agctggacta aaaggcatgc aatttcataa tcaaagagag cgaaaaagta     3480

gaacgaatga tgatattgac catgagcgaa cacgtgaaaa ttatgatttg aaaaatgata     3540

aaaatattga ttacaacgaa cgtgtcaaag aaattattga atcacaaaaa acaggtacaa     3600

gaaaaacgag gaaagatgct gttcttgtaa atgagttgct agtaacatct gaccgagatt     3660

tttttgagca actggatcct gataggtggt atgttttcgc ttgaactttt aaatacagcc     3720

attgaacata cggttgattt aataactgac aaacatcacc ctcttgctaa agcggccaag     3780

gacgctgccg ccggggctgt ttgcgttttt gccgtgattt cgtgtatcat tggtttactt     3840

atttttttgc caaagctgta atggctgaaa attcttacat ttattttaca tttttagaaa     3900

tgggcgtgaa aaaaagcgcg cgattatgta aaatataaag tgatagcggt acccatatgc     3960

accaccacca ccaccacagc ggcgcttttg aatttaagct gccggacatt ggcgaaggca     4020

tccacgaagg tgaaattgtc aaatggtttg tgaaaccggg cgatgaagtg aacgaagacg     4080

atgtattgtg cgaagtgcaa aatgacaagg cggttgtcga aattccctcc ccggtcaaag     4140

ggaaagtgct tgaaatcctc gtcccggagg gaacagtggc aacggtcggg caaacgctca     4200

tcacgctcga tgcgccgggt tatgaaaaca tgacgaccgg cagcgacacc ggcgaaaacc     4260

tgtacttcca gggtggatcc atgtctgttg ctttttctga taagcaagaa gcattggtta     4320

atggttcatg ggaagccttt aaaactaaca tcccaaagta ctctgttgtt ttctatacta     4380

caattttaga aaaagctcca gctgctaaaa atttgttttc atttttggca aatggtgttg     4440

atccatcaaa tccaaaattg ttagctcatg cagaaaaatt gttcggttta gttagagatt     4500

ctgctgcaca attgagagct tcaggtacag ttgttgcaga tgctgcatta ggttctgttc     4560

attcacaaaa agctgttact gatgcacaat tcttggttgt taaagaagca ttggttaaga     4620

cattgaaaga agcagttggt gacaaatggt cagatgaatt aactacagct gttgaattgg     4680

cttatgatga attagctgtt gctattaaaa aggcttatta agaattcgag ctccgtcgac     4740

ctcgaggggc tagccgctgc agttgaattc aagcttggcg taatcatggt catagctgtt     4800

tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa     4860

gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact     4920

gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc     4980

ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg     5040

ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc     5100

cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag     5160

gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     5220

tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     5280

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     5340

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     5400

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     5460

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     5520

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     5580

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     5640

tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc     5700

cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg     5760

cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg     5820

gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta     5880

gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg     5940

gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg     6000

ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc     6060

atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc     6120

agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc     6180

ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag     6240

tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat     6300

ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg     6360

caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt     6420

gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag     6480

atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg     6540

accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt     6600

aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct     6660

gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac     6720

tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat     6780

aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat     6840

ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca     6900

aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat     6960

tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc                7010


