                         SEQUENCE LISTING

<110>  Han, Jang
 
<120>  METHODS AND COMPOSITIONS FOR TREATING SARS-CoV-2 INFECTION

<130>  HAN0002-101-US

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  216
<212>  DNA
<213>  SARS-CoV

<400>  1
aattctaaca atcttgattc taaggttggt ggtaattata attacctgta tagattgttt       60

aggaagtcta atctcaaacc ttttgagaga gatatttcaa ctgaaatcta tcaggccggt      120

agcacacctt gtaatggtgt tgaaggtttt aattgttact ttcctttaca atcatatggt      180

ttccaaccca ctaatggtgt tggttaccaa ccatac                                216


<210>  2
<211>  72
<212>  PRT
<213>  SARS-CoV

<400>  2

Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu 
1               5                   10                  15      


Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile 
            20                  25                  30          


Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu 
        35                  40                  45              


Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr 
    50                  55                  60                  


Asn Gly Val Gly Tyr Gln Pro Tyr 
65                  70          


<210>  3
<211>  2136
<212>  DNA
<213>  Homo sapiens

<400>  3
atgaaacttg tcttcctcgt cctgctgttc ctcggggccc tcggactgtg tctggctggc       60

cgtaggagaa ggagtgttca gtggtgcgcc gtatcccaac ccgaggccac aaaatgcttc      120

caatggcaaa ggaatatgag aaaagtgcgt ggccctcctg tcagctgcat aaagagagac      180

tcccccatcc agtgtatcca ggccattgcg gaaaacaggg ccgatgctgt gacccttgat      240

ggtggtttca tatacgaggc aggcctggcc ccctacaaac tgcgacctgt agcggcggaa      300

gtctacggga ccgaaagaca gccacgaact cactattatg ccgtggctgt ggtgaagaag      360

ggcggcagct ttcagctgaa cgaactgcaa ggtctgaagt cctgccacac aggccttcgc      420

aggaccgctg gatggaatgt ccctataggg acacttcgtc cattcttgaa ttggacgggt      480

ccacctgagc ccattgaggc agctgtggcc aggttcttct cagccagctg tgttcccggt      540

gcagataaag gacagttccc caacctgtgt cgcctgtgtg cggggacagg ggaaaacaaa      600

tgtgccttct cctcccagga accgtacttc agctactctg gtgccttcaa gtgtctgaga      660

gacggggctg gagacgtggc ttttatcaga gagagcacag tgtttgagga cctgtcagac      720

gaggctgaaa gggacgagta tgagttactc tgcccagaca acactcggaa gccagtggac      780

aagttcaaag actgccatct ggcccgggtc ccttctcatg ccgttgtggc acgaagtgtg      840

aatggcaagg aggatgccat ctggaatctt ctccgccagg cacaggaaaa gtttggaaag      900

gacaagtcac cgaaattcca gctctttggc tcccctagtg ggcagaaaga tctgctgttc      960

aaggactctg ccattgggtt ttcgagggtg cccccgagga tagattctgg gctgtacctt     1020

ggctccggct acttcactgc catccagaac ttgaggaaaa gtgaggagga agtggctgcc     1080

cggcgtgcgc gggtcgtgtg gtgtgcggtg ggcgagcagg agctgcgcaa gtgtaaccag     1140

tggagtggct tgagcgaagg cagcgtgacc tgctcctcgg cctccaccac agaggactgc     1200

atcgccctgg tgctgaaagg agaagctgat gccatgagtt tggatgaagg atatgtgtac     1260

actgcaggca aatgtggttt ggtgcctgtc ctggcagaga actacaaatc ccaacaaagc     1320

agtgaccctg atcctaactg tgtggataga cctgtggaag gatatcttgc tgtggcggtg     1380

gttaggagat cagacactag ccttacctgg aactctgtga aaggcaagaa gtcctgccac     1440

accgccgtgg acaggactgc aggctggaat atccccatgg gcctgctctt caaccagacg     1500

ggctcctgca aatttgatga atatttcagt caaagctgtg cccctgggtc tgacccgaga     1560

tctaatctct gtgctctgtg tattggcgac gagcagggtg agaataagtg cgtgcccaac     1620

agcaacgaga gatactacgg ctacactggg gctttccggt gcctggctga gaatgctgga     1680

gacgttgcat ttgtgaaaga tgtcactgtc ttgcagaaca ctgatggaaa taacaatgag     1740

gcatgggcta aggatttgaa gctggcagac tttgcgctgc tgtgcctcga tggcaaacgg     1800

aagcctgtga ctgaggctag aagctgccat cttgccatgg ccccgaatca tgccgtggtg     1860

tctcggatgg ataaggtgga acgcctgaaa caggtgttgc tccaccaaca ggctaaattt     1920

gggagaaatg gatctgactg cccggacaag ttttgcttat tccagtctga aaccaaaaac     1980

cttctgttca atgacaacac tgagtgtctg gccagactcc atggcaaaac aacatatgaa     2040

aaatatttgg gaccacagta tgtcgcaggc attactaatc tgaaaaagtg ctcaacctcc     2100

cccctcctgg aagcctgtga attcctcagg aagtaa                               2136


<210>  4
<211>  711
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Lys Leu Val Phe Leu Val Leu Leu Phe Leu Gly Ala Leu Gly Leu 
1               5                   10                  15      


Cys Leu Ala Gly Arg Arg Arg Arg Ser Val Gln Trp Cys Ala Val Ser 
            20                  25                  30          


Gln Pro Glu Ala Thr Lys Cys Phe Gln Trp Gln Arg Asn Met Arg Lys 
        35                  40                  45              


Val Arg Gly Pro Pro Val Ser Cys Ile Lys Arg Asp Ser Pro Ile Gln 
    50                  55                  60                  


Cys Ile Gln Ala Ile Ala Glu Asn Arg Ala Asp Ala Val Thr Leu Asp 
65                  70                  75                  80  


Gly Gly Phe Ile Tyr Glu Ala Gly Leu Ala Pro Tyr Lys Leu Arg Pro 
                85                  90                  95      


Val Ala Ala Glu Val Tyr Gly Thr Glu Arg Gln Pro Arg Thr His Tyr 
            100                 105                 110         


Tyr Ala Val Ala Val Val Lys Lys Gly Gly Ser Phe Gln Leu Asn Glu 
        115                 120                 125             


Leu Gln Gly Leu Lys Ser Cys His Thr Gly Leu Arg Arg Thr Ala Gly 
    130                 135                 140                 


Trp Asn Val Pro Ile Gly Thr Leu Arg Pro Phe Leu Asn Trp Thr Gly 
145                 150                 155                 160 


Pro Pro Glu Pro Ile Glu Ala Ala Val Ala Arg Phe Phe Ser Ala Ser 
                165                 170                 175     


Cys Val Pro Gly Ala Asp Lys Gly Gln Phe Pro Asn Leu Cys Arg Leu 
            180                 185                 190         


Cys Ala Gly Thr Gly Glu Asn Lys Cys Ala Phe Ser Ser Gln Glu Pro 
        195                 200                 205             


Tyr Phe Ser Tyr Ser Gly Ala Phe Lys Cys Leu Arg Asp Gly Ala Gly 
    210                 215                 220                 


Asp Val Ala Phe Ile Arg Glu Ser Thr Val Phe Glu Asp Leu Ser Asp 
225                 230                 235                 240 


Glu Ala Glu Arg Asp Glu Tyr Glu Leu Leu Cys Pro Asp Asn Thr Arg 
                245                 250                 255     


Lys Pro Val Asp Lys Phe Lys Asp Cys His Leu Ala Arg Val Pro Ser 
            260                 265                 270         


His Ala Val Val Ala Arg Ser Val Asn Gly Lys Glu Asp Ala Ile Trp 
        275                 280                 285             


Asn Leu Leu Arg Gln Ala Gln Glu Lys Phe Gly Lys Asp Lys Ser Pro 
    290                 295                 300                 


Lys Phe Gln Leu Phe Gly Ser Pro Ser Gly Gln Lys Asp Leu Leu Phe 
305                 310                 315                 320 


Lys Asp Ser Ala Ile Gly Phe Ser Arg Val Pro Pro Arg Ile Asp Ser 
                325                 330                 335     


Gly Leu Tyr Leu Gly Ser Gly Tyr Phe Thr Ala Ile Gln Asn Leu Arg 
            340                 345                 350         


Lys Ser Glu Glu Glu Val Ala Ala Arg Arg Ala Arg Val Val Trp Cys 
        355                 360                 365             


Ala Val Gly Glu Gln Glu Leu Arg Lys Cys Asn Gln Trp Ser Gly Leu 
    370                 375                 380                 


Ser Glu Gly Ser Val Thr Cys Ser Ser Ala Ser Thr Thr Glu Asp Cys 
385                 390                 395                 400 


Ile Ala Leu Val Leu Lys Gly Glu Ala Asp Ala Met Ser Leu Asp Glu 
                405                 410                 415     


Gly Tyr Val Tyr Thr Ala Gly Lys Cys Gly Leu Val Pro Val Leu Ala 
            420                 425                 430         


Glu Asn Tyr Lys Ser Gln Gln Ser Ser Asp Pro Asp Pro Asn Cys Val 
        435                 440                 445             


Asp Arg Pro Val Glu Gly Tyr Leu Ala Val Ala Val Val Arg Arg Ser 
    450                 455                 460                 


Asp Thr Ser Leu Thr Trp Asn Ser Val Lys Gly Lys Lys Ser Cys His 
465                 470                 475                 480 


Thr Ala Val Asp Arg Thr Ala Gly Trp Asn Ile Pro Met Gly Leu Leu 
                485                 490                 495     


Phe Asn Gln Thr Gly Ser Cys Lys Phe Asp Glu Tyr Phe Ser Gln Ser 
            500                 505                 510         


Cys Ala Pro Gly Ser Asp Pro Arg Ser Asn Leu Cys Ala Leu Cys Ile 
        515                 520                 525             


Gly Asp Glu Gln Gly Glu Asn Lys Cys Val Pro Asn Ser Asn Glu Arg 
    530                 535                 540                 


Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Ala Glu Asn Ala Gly 
545                 550                 555                 560 


Asp Val Ala Phe Val Lys Asp Val Thr Val Leu Gln Asn Thr Asp Gly 
                565                 570                 575     


Asn Asn Asn Glu Ala Trp Ala Lys Asp Leu Lys Leu Ala Asp Phe Ala 
            580                 585                 590         


Leu Leu Cys Leu Asp Gly Lys Arg Lys Pro Val Thr Glu Ala Arg Ser 
        595                 600                 605             


Cys His Leu Ala Met Ala Pro Asn His Ala Val Val Ser Arg Met Asp 
    610                 615                 620                 


Lys Val Glu Arg Leu Lys Gln Val Leu Leu His Gln Gln Ala Lys Phe 
625                 630                 635                 640 


Gly Arg Asn Gly Ser Asp Cys Pro Asp Lys Phe Cys Leu Phe Gln Ser 
                645                 650                 655     


Glu Thr Lys Asn Leu Leu Phe Asn Asp Asn Thr Glu Cys Leu Ala Arg 
            660                 665                 670         


Leu His Gly Lys Thr Thr Tyr Glu Lys Tyr Leu Gly Pro Gln Tyr Val 
        675                 680                 685             


Ala Gly Ile Thr Asn Leu Lys Lys Cys Ser Thr Ser Pro Leu Leu Glu 
    690                 695                 700                 


Ala Cys Glu Phe Leu Arg Lys 
705                 710     


<210>  5
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  4-glycine linker

<400>  5
ggcggcggcg gc                                                           12


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-glycine linker

<400>  6

Gly Gly Gly Gly 
1               


<210>  7
<211>  2817
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of hLF-CoV2-RBD construct

<400>  7
atgaaacttg tcttcctcgt cctgctgttc ctcggggccc tcggactgtg tctggctggc       60

cgtaggagaa ggagtgttca gtggtgcgcc gtatcccaac ccgaggccac aaaatgcttc      120

caatggcaaa ggaatatgag aaaagtgcgt ggccctcctg tcagctgcat aaagagagac      180

tcccccatcc agtgtatcca ggccattgcg gaaaacaggg ccgatgctgt gacccttgat      240

ggtggtttca tatacgaggc aggcctggcc ccctacaaac tgcgacctgt agcggcggaa      300

gtctacggga ccgaaagaca gccacgaact cactattatg ccgtggctgt ggtgaagaag      360

ggcggcagct ttcagctgaa cgaactgcaa ggtctgaagt cctgccacac aggccttcgc      420

aggaccgctg gatggaatgt ccctataggg acacttcgtc cattcttgaa ttggacgggt      480

ccacctgagc ccattgaggc agctgtggcc aggttcttct cagccagctg tgttcccggt      540

gcagataaag gacagttccc caacctgtgt cgcctgtgtg cggggacagg ggaaaacaaa      600

tgtgccttct cctcccagga accgtacttc agctactctg gtgccttcaa gtgtctgaga      660

gacggggctg gagacgtggc ttttatcaga gagagcacag tgtttgagga cctgtcagac      720

gaggctgaaa gggacgagta tgagttactc tgcccagaca acactcggaa gccagtggac      780

aagttcaaag actgccatct ggcccgggtc ccttctcatg ccgttgtggc acgaagtgtg      840

aatggcaagg aggatgccat ctggaatctt ctccgccagg cacaggaaaa gtttggaaag      900

gacaagtcac cgaaattcca gctctttggc tcccctagtg ggcagaaaga tctgctgttc      960

aaggactctg ccattgggtt ttcgagggtg cccccgagga tagattctgg gctgtacctt     1020

ggctccggct acttcactgc catccagaac ttgaggaaaa gtgaggagga agtggctgcc     1080

cggcgtgcgc gggtcgtgtg gtgtgcggtg ggcgagcagg agctgcgcaa gtgtaaccag     1140

tggagtggct tgagcgaagg cagcgtgacc tgctcctcgg cctccaccac agaggactgc     1200

atcgccctgg tgctgaaagg agaagctgat gccatgagtt tggatgaagg atatgtgtac     1260

actgcaggca aatgtggttt ggtgcctgtc ctggcagaga actacaaatc ccaacaaagc     1320

agtgaccctg atcctaactg tgtggataga cctgtggaag gatatcttgc tgtggcggtg     1380

gttaggagat cagacactag ccttacctgg aactctgtga aaggcaagaa gtcctgccac     1440

accgccgtgg acaggactgc aggctggaat atccccatgg gcctgctctt caaccagacg     1500

ggctcctgca aatttgatga atatttcagt caaagctgtg cccctgggtc tgacccgaga     1560

tctaatctct gtgctctgtg tattggcgac gagcagggtg agaataagtg cgtgcccaac     1620

agcaacgaga gatactacgg ctacactggg gctttccggt gcctggctga gaatgctgga     1680

gacgttgcat ttgtgaaaga tgtcactgtc ttgcagaaca ctgatggaaa taacaatgag     1740

gcatgggcta aggatttgaa gctggcagac tttgcgctgc tgtgcctcga tggcaaacgg     1800

aagcctgtga ctgaggctag aagctgccat cttgccatgg ccccgaatca tgccgtggtg     1860

tctcggatgg ataaggtgga acgcctgaaa caggtgttgc tccaccaaca ggctaaattt     1920

gggagaaatg gatctgactg cccggacaag ttttgcttat tccagtctga aaccaaaaac     1980

cttctgttca atgacaacac tgagtgtctg gccagactcc atggcaaaac aacatatgaa     2040

aaatatttgg gaccacagta tgtcgcaggc attactaatc tgaaaaagtg ctcaacctcc     2100

cccctcctgg aagcctgtga attcctcagg aagggcggcg gcggcagggt ccaaccaaca     2160

gagagcattg tgaggtttcc aaacatcacc aacctgtgtc catttggaga ggtgttcaat     2220

gccaccaggt ttgcctctgt ctatgcctgg aacaggaaga ggattagcaa ctgtgtggct     2280

gactactctg tgctctacaa ctctgcctcc ttcagcacct tcaagtgtta tggagtgagc     2340

ccaaccaaac tgaatgacct gtgtttcacc aatgtctatg ctgactcctt tgtgattagg     2400

ggagatgagg tgagacagat tgcccctgga caaacaggca agattgctga ctacaactac     2460

aaactgcctg atgacttcac aggctgtgtg attgcctgga acagcaacaa cctggacagc     2520

aaggtgggag gcaactacaa ctacctctac agactgttca ggaagagcaa cctgaaacca     2580

tttgagaggg acatcagcac agagatttac caggctggca gcacaccatg taatggagtg     2640

gagggcttca actgttactt tccactccaa tcctatggct tccaaccaac caatggagtg     2700

ggctaccaac catacagggt ggtggtgctg tcctttgaac tgctccatgc ccctgccaca     2760

gtgtgtggac caaagaagag caccaacctg gtgaagaaca agtgtgtgaa cttctaa        2817


<210>  8
<211>  938
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of hLF-CoV2-RBD construct

<400>  8

Met Lys Leu Val Phe Leu Val Leu Leu Phe Leu Gly Ala Leu Gly Leu 
1               5                   10                  15      


Cys Leu Ala Gly Arg Arg Arg Arg Ser Val Gln Trp Cys Ala Val Ser 
            20                  25                  30          


Gln Pro Glu Ala Thr Lys Cys Phe Gln Trp Gln Arg Asn Met Arg Lys 
        35                  40                  45              


Val Arg Gly Pro Pro Val Ser Cys Ile Lys Arg Asp Ser Pro Ile Gln 
    50                  55                  60                  


Cys Ile Gln Ala Ile Ala Glu Asn Arg Ala Asp Ala Val Thr Leu Asp 
65                  70                  75                  80  


Gly Gly Phe Ile Tyr Glu Ala Gly Leu Ala Pro Tyr Lys Leu Arg Pro 
                85                  90                  95      


Val Ala Ala Glu Val Tyr Gly Thr Glu Arg Gln Pro Arg Thr His Tyr 
            100                 105                 110         


Tyr Ala Val Ala Val Val Lys Lys Gly Gly Ser Phe Gln Leu Asn Glu 
        115                 120                 125             


Leu Gln Gly Leu Lys Ser Cys His Thr Gly Leu Arg Arg Thr Ala Gly 
    130                 135                 140                 


Trp Asn Val Pro Ile Gly Thr Leu Arg Pro Phe Leu Asn Trp Thr Gly 
145                 150                 155                 160 


Pro Pro Glu Pro Ile Glu Ala Ala Val Ala Arg Phe Phe Ser Ala Ser 
                165                 170                 175     


Cys Val Pro Gly Ala Asp Lys Gly Gln Phe Pro Asn Leu Cys Arg Leu 
            180                 185                 190         


Cys Ala Gly Thr Gly Glu Asn Lys Cys Ala Phe Ser Ser Gln Glu Pro 
        195                 200                 205             


Tyr Phe Ser Tyr Ser Gly Ala Phe Lys Cys Leu Arg Asp Gly Ala Gly 
    210                 215                 220                 


Asp Val Ala Phe Ile Arg Glu Ser Thr Val Phe Glu Asp Leu Ser Asp 
225                 230                 235                 240 


Glu Ala Glu Arg Asp Glu Tyr Glu Leu Leu Cys Pro Asp Asn Thr Arg 
                245                 250                 255     


Lys Pro Val Asp Lys Phe Lys Asp Cys His Leu Ala Arg Val Pro Ser 
            260                 265                 270         


His Ala Val Val Ala Arg Ser Val Asn Gly Lys Glu Asp Ala Ile Trp 
        275                 280                 285             


Asn Leu Leu Arg Gln Ala Gln Glu Lys Phe Gly Lys Asp Lys Ser Pro 
    290                 295                 300                 


Lys Phe Gln Leu Phe Gly Ser Pro Ser Gly Gln Lys Asp Leu Leu Phe 
305                 310                 315                 320 


Lys Asp Ser Ala Ile Gly Phe Ser Arg Val Pro Pro Arg Ile Asp Ser 
                325                 330                 335     


Gly Leu Tyr Leu Gly Ser Gly Tyr Phe Thr Ala Ile Gln Asn Leu Arg 
            340                 345                 350         


Lys Ser Glu Glu Glu Val Ala Ala Arg Arg Ala Arg Val Val Trp Cys 
        355                 360                 365             


Ala Val Gly Glu Gln Glu Leu Arg Lys Cys Asn Gln Trp Ser Gly Leu 
    370                 375                 380                 


Ser Glu Gly Ser Val Thr Cys Ser Ser Ala Ser Thr Thr Glu Asp Cys 
385                 390                 395                 400 


Ile Ala Leu Val Leu Lys Gly Glu Ala Asp Ala Met Ser Leu Asp Glu 
                405                 410                 415     


Gly Tyr Val Tyr Thr Ala Gly Lys Cys Gly Leu Val Pro Val Leu Ala 
            420                 425                 430         


Glu Asn Tyr Lys Ser Gln Gln Ser Ser Asp Pro Asp Pro Asn Cys Val 
        435                 440                 445             


Asp Arg Pro Val Glu Gly Tyr Leu Ala Val Ala Val Val Arg Arg Ser 
    450                 455                 460                 


Asp Thr Ser Leu Thr Trp Asn Ser Val Lys Gly Lys Lys Ser Cys His 
465                 470                 475                 480 


Thr Ala Val Asp Arg Thr Ala Gly Trp Asn Ile Pro Met Gly Leu Leu 
                485                 490                 495     


Phe Asn Gln Thr Gly Ser Cys Lys Phe Asp Glu Tyr Phe Ser Gln Ser 
            500                 505                 510         


Cys Ala Pro Gly Ser Asp Pro Arg Ser Asn Leu Cys Ala Leu Cys Ile 
        515                 520                 525             


Gly Asp Glu Gln Gly Glu Asn Lys Cys Val Pro Asn Ser Asn Glu Arg 
    530                 535                 540                 


Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Ala Glu Asn Ala Gly 
545                 550                 555                 560 


Asp Val Ala Phe Val Lys Asp Val Thr Val Leu Gln Asn Thr Asp Gly 
                565                 570                 575     


Asn Asn Asn Glu Ala Trp Ala Lys Asp Leu Lys Leu Ala Asp Phe Ala 
            580                 585                 590         


Leu Leu Cys Leu Asp Gly Lys Arg Lys Pro Val Thr Glu Ala Arg Ser 
        595                 600                 605             


Cys His Leu Ala Met Ala Pro Asn His Ala Val Val Ser Arg Met Asp 
    610                 615                 620                 


Lys Val Glu Arg Leu Lys Gln Val Leu Leu His Gln Gln Ala Lys Phe 
625                 630                 635                 640 


Gly Arg Asn Gly Ser Asp Cys Pro Asp Lys Phe Cys Leu Phe Gln Ser 
                645                 650                 655     


Glu Thr Lys Asn Leu Leu Phe Asn Asp Asn Thr Glu Cys Leu Ala Arg 
            660                 665                 670         


Leu His Gly Lys Thr Thr Tyr Glu Lys Tyr Leu Gly Pro Gln Tyr Val 
        675                 680                 685             


Ala Gly Ile Thr Asn Leu Lys Lys Cys Ser Thr Ser Pro Leu Leu Glu 
    690                 695                 700                 


Ala Cys Glu Phe Leu Arg Lys Gly Gly Gly Gly Arg Val Gln Pro Thr 
705                 710                 715                 720 


Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly 
                725                 730                 735     


Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg 
            740                 745                 750         


Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser 
        755                 760                 765             


Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu 
    770                 775                 780                 


Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg 
785                 790                 795                 800 


Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala 
                805                 810                 815     


Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala 
            820                 825                 830         


Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr 
        835                 840                 845             


Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp 
    850                 855                 860                 


Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val 
865                 870                 875                 880 


Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro 
                885                 890                 895     


Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe 
            900                 905                 910         


Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr 
        915                 920                 925             


Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    930                 935             


<210>  9
<211>  3546
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA sequence of LF-Fc-RBD construct

<400>  9
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg       60

ggctcgaggg gccgtaggag aaggagtgtt cagtggtgcg ccgtatccca acccgaggcc      120

acaaaatgct tccaatggca aaggaatatg agaaaagtgc gtggccctcc tgtcagctgc      180

ataaagagag actcccccat ccagtgtatc caggccattg cggaaaacag ggccgatgct      240

gtgacccttg atggtggttt catatacgag gcaggcctgg ccccctacaa actgcgacct      300

gtagcggcgg aagtctacgg gaccgaaaga cagccacgaa ctcactatta tgccgtggct      360

gtggtgaaga agggcggcag ctttcagctg aacgaactgc aaggtctgaa gtcctgccac      420

acaggccttc gcaggaccgc tggatggaat gtccctatag ggacacttcg tccattcttg      480

aattggacgg gtccacctga gcccattgag gcagctgtgg ccaggttctt ctcagccagc      540

tgtgttcccg gtgcagataa aggacagttc cccaacctgt gtcgcctgtg tgcggggaca      600

ggggaaaaca aatgtgcctt ctcctcccag gaaccgtact tcagctactc tggtgccttc      660

aagtgtctga gagacggggc tggagacgtg gcttttatca gagagagcac agtgtttgag      720

gacctgtcag acgaggctga aagggacgag tatgagttac tctgcccaga caacactcgg      780

aagccagtgg acaagttcaa agactgccat ctggcccggg tcccttctca tgccgttgtg      840

gcacgaagtg tgaatggcaa ggaggatgcc atctggaatc ttctccgcca ggcacaggaa      900

aagtttggaa aggacaagtc accgaaattc cagctctttg gctcccctag tgggcagaaa      960

gatctgctgt tcaaggactc tgccattggg ttttcgaggg tgcccccgag gatagattct     1020

gggctgtacc ttggctccgg ctacttcact gccatccaga acttgaggaa aagtgaggag     1080

gaagtggctg cccggcgtgc gcgggtcgtg tggtgtgcgg tgggcgagca ggagctgcgc     1140

aagtgtaacc agtggagtgg cttgagcgaa ggcagcgtga cctgctcctc ggcctccacc     1200

acagaggact gcatcgccct ggtgctgaaa ggagaagctg atgccatgag tttggatgaa     1260

ggatatgtgt acactgcagg caaatgtggt ttggtgcctg tcctggcaga gaactacaaa     1320

tcccaacaaa gcagtgaccc tgatcctaac tgtgtggata gacctgtgga aggatatctt     1380

gctgtggcgg tggttaggag atcagacact agccttacct ggaactctgt gaaaggcaag     1440

aagtcctgcc acaccgccgt ggacaggact gcaggctgga atatccccat gggcctgctc     1500

ttcaaccaga cgggctcctg caaatttgat gaatatttca gtcaaagctg tgcccctggg     1560

tctgacccga gatctaatct ctgtgctctg tgtattggcg acgagcaggg tgagaataag     1620

tgcgtgccca acagcaacga gagatactac ggctacactg gggctttccg gtgcctggct     1680

gagaatgctg gagacgttgc atttgtgaaa gatgtcactg tcttgcagaa cactgatgga     1740

aataacaatg aggcatgggc taaggatttg aagctggcag actttgcgct gctgtgcctc     1800

gatggcaaac ggaagcctgt gactgaggct agaagctgcc atcttgccat ggccccgaat     1860

catgccgtgg tgtctcggat ggataaggtg gaacgcctga aacaggtgtt gctccaccaa     1920

caggctaaat ttgggagaaa tggatctgac tgcccggaca agttttgctt attccagtct     1980

gaaaccaaaa accttctgtt caatgacaac actgagtgtc tggccagact ccatggcaaa     2040

acaacatatg aaaaatattt gggaccacag tatgtcgcag gcattactaa tctgaaaaag     2100

tgctcaacct cccccctcct ggaagcctgt gaattcctca ggaaggggcc ctcgggctcg     2160

agtgctgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc agcacctgaa     2220

ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac cctcatgatc     2280

tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc     2340

aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa gccgcgggag     2400

gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg     2460

ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag     2520

aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac cctgccccca     2580

tcccgggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctat     2640

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc     2700

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct caccgtggac     2760

aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga ggctctgcac     2820

aaccactaca cgcagaagag cctctccctg tctccgggta aaggcggcgg cggcagggtc     2880

caaccaacag agagcattgt gaggtttcca aacatcacca acctgtgtcc atttggagag     2940

gtgttcaatg ccaccaggtt tgcctctgtc tatgcctgga acaggaagag gattagcaac     3000

tgtgtggctg actactctgt gctctacaac tctgcctcct tcagcacctt caagtgttat     3060

ggagtgagcc caaccaaact gaatgacctg tgtttcacca atgtctatgc tgactccttt     3120

gtgattaggg gagatgaggt gagacagatt gcccctggac aaacaggcaa gattgctgac     3180

tacaactaca aactgcctga tgacttcaca ggctgtgtga ttgcctggaa cagcaacaac     3240

ctggacagca aggtgggagg caactacaac tacctctaca gactgttcag gaagagcaac     3300

ctgaaaccat ttgagaggga catcagcaca gagatttacc aggctggcag cacaccatgt     3360

aatggagtgg agggcttcaa ctgttacttt ccactccaat cctatggctt ccaaccaacc     3420

aatggagtgg gctaccaacc atacagggtg gtggtgctgt cctttgaact gctccatgcc     3480

cctgccacag tgtgtggacc aaagaagagc accaacctgg tgaagaacaa gtgtgtgaac     3540

ttctag                                                                3546


<210>  10
<211>  1181
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of LF-Fc-RBD construct

<400>  10

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Gly Ser Arg Gly Arg Arg Arg Arg Ser Val Gln Trp 
            20                  25                  30          


Cys Ala Val Ser Gln Pro Glu Ala Thr Lys Cys Phe Gln Trp Gln Arg 
        35                  40                  45              


Asn Met Arg Lys Val Arg Gly Pro Pro Val Ser Cys Ile Lys Arg Asp 
    50                  55                  60                  


Ser Pro Ile Gln Cys Ile Gln Ala Ile Ala Glu Asn Arg Ala Asp Ala 
65                  70                  75                  80  


Val Thr Leu Asp Gly Gly Phe Ile Tyr Glu Ala Gly Leu Ala Pro Tyr 
                85                  90                  95      


Lys Leu Arg Pro Val Ala Ala Glu Val Tyr Gly Thr Glu Arg Gln Pro 
            100                 105                 110         


Arg Thr His Tyr Tyr Ala Val Ala Val Val Lys Lys Gly Gly Ser Phe 
        115                 120                 125             


Gln Leu Asn Glu Leu Gln Gly Leu Lys Ser Cys His Thr Gly Leu Arg 
    130                 135                 140                 


Arg Thr Ala Gly Trp Asn Val Pro Ile Gly Thr Leu Arg Pro Phe Leu 
145                 150                 155                 160 


Asn Trp Thr Gly Pro Pro Glu Pro Ile Glu Ala Ala Val Ala Arg Phe 
                165                 170                 175     


Phe Ser Ala Ser Cys Val Pro Gly Ala Asp Lys Gly Gln Phe Pro Asn 
            180                 185                 190         


Leu Cys Arg Leu Cys Ala Gly Thr Gly Glu Asn Lys Cys Ala Phe Ser 
        195                 200                 205             


Ser Gln Glu Pro Tyr Phe Ser Tyr Ser Gly Ala Phe Lys Cys Leu Arg 
    210                 215                 220                 


Asp Gly Ala Gly Asp Val Ala Phe Ile Arg Glu Ser Thr Val Phe Glu 
225                 230                 235                 240 


Asp Leu Ser Asp Glu Ala Glu Arg Asp Glu Tyr Glu Leu Leu Cys Pro 
                245                 250                 255     


Asp Asn Thr Arg Lys Pro Val Asp Lys Phe Lys Asp Cys His Leu Ala 
            260                 265                 270         


Arg Val Pro Ser His Ala Val Val Ala Arg Ser Val Asn Gly Lys Glu 
        275                 280                 285             


Asp Ala Ile Trp Asn Leu Leu Arg Gln Ala Gln Glu Lys Phe Gly Lys 
    290                 295                 300                 


Asp Lys Ser Pro Lys Phe Gln Leu Phe Gly Ser Pro Ser Gly Gln Lys 
305                 310                 315                 320 


Asp Leu Leu Phe Lys Asp Ser Ala Ile Gly Phe Ser Arg Val Pro Pro 
                325                 330                 335     


Arg Ile Asp Ser Gly Leu Tyr Leu Gly Ser Gly Tyr Phe Thr Ala Ile 
            340                 345                 350         


Gln Asn Leu Arg Lys Ser Glu Glu Glu Val Ala Ala Arg Arg Ala Arg 
        355                 360                 365             


Val Val Trp Cys Ala Val Gly Glu Gln Glu Leu Arg Lys Cys Asn Gln 
    370                 375                 380                 


Trp Ser Gly Leu Ser Glu Gly Ser Val Thr Cys Ser Ser Ala Ser Thr 
385                 390                 395                 400 


Thr Glu Asp Cys Ile Ala Leu Val Leu Lys Gly Glu Ala Asp Ala Met 
                405                 410                 415     


Ser Leu Asp Glu Gly Tyr Val Tyr Thr Ala Gly Lys Cys Gly Leu Val 
            420                 425                 430         


Pro Val Leu Ala Glu Asn Tyr Lys Ser Gln Gln Ser Ser Asp Pro Asp 
        435                 440                 445             


Pro Asn Cys Val Asp Arg Pro Val Glu Gly Tyr Leu Ala Val Ala Val 
    450                 455                 460                 


Val Arg Arg Ser Asp Thr Ser Leu Thr Trp Asn Ser Val Lys Gly Lys 
465                 470                 475                 480 


Lys Ser Cys His Thr Ala Val Asp Arg Thr Ala Gly Trp Asn Ile Pro 
                485                 490                 495     


Met Gly Leu Leu Phe Asn Gln Thr Gly Ser Cys Lys Phe Asp Glu Tyr 
            500                 505                 510         


Phe Ser Gln Ser Cys Ala Pro Gly Ser Asp Pro Arg Ser Asn Leu Cys 
        515                 520                 525             


Ala Leu Cys Ile Gly Asp Glu Gln Gly Glu Asn Lys Cys Val Pro Asn 
    530                 535                 540                 


Ser Asn Glu Arg Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Ala 
545                 550                 555                 560 


Glu Asn Ala Gly Asp Val Ala Phe Val Lys Asp Val Thr Val Leu Gln 
                565                 570                 575     


Asn Thr Asp Gly Asn Asn Asn Glu Ala Trp Ala Lys Asp Leu Lys Leu 
            580                 585                 590         


Ala Asp Phe Ala Leu Leu Cys Leu Asp Gly Lys Arg Lys Pro Val Thr 
        595                 600                 605             


Glu Ala Arg Ser Cys His Leu Ala Met Ala Pro Asn His Ala Val Val 
    610                 615                 620                 


Ser Arg Met Asp Lys Val Glu Arg Leu Lys Gln Val Leu Leu His Gln 
625                 630                 635                 640 


Gln Ala Lys Phe Gly Arg Asn Gly Ser Asp Cys Pro Asp Lys Phe Cys 
                645                 650                 655     


Leu Phe Gln Ser Glu Thr Lys Asn Leu Leu Phe Asn Asp Asn Thr Glu 
            660                 665                 670         


Cys Leu Ala Arg Leu His Gly Lys Thr Thr Tyr Glu Lys Tyr Leu Gly 
        675                 680                 685             


Pro Gln Tyr Val Ala Gly Ile Thr Asn Leu Lys Lys Cys Ser Thr Ser 
    690                 695                 700                 


Pro Leu Leu Glu Ala Cys Glu Phe Leu Arg Lys Gly Pro Ser Gly Ser 
705                 710                 715                 720 


Ser Ala Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
                725                 730                 735     


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
            740                 745                 750         


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
        755                 760                 765             


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
    770                 775                 780                 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
785                 790                 795                 800 


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
                805                 810                 815     


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
            820                 825                 830         


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
        835                 840                 845             


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu 
    850                 855                 860                 


Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
865                 870                 875                 880 


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
                885                 890                 895     


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
            900                 905                 910         


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
        915                 920                 925             


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
    930                 935                 940                 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Gly Gly Gly Gly Arg Val 
945                 950                 955                 960 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                965                 970                 975     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            980                 985                 990         


Trp Asn Arg Lys Arg Ile Ser Asn  Cys Val Ala Asp Tyr  Ser Val Leu 
        995                 1000                 1005             


Tyr Asn  Ser Ala Ser Phe Ser  Thr Phe Lys Cys Tyr  Gly Val Ser 
    1010                 1015                 1020             


Pro Thr  Lys Leu Asn Asp Leu  Cys Phe Thr Asn Val  Tyr Ala Asp 
    1025                 1030                 1035             


Ser Phe  Val Ile Arg Gly Asp  Glu Val Arg Gln Ile  Ala Pro Gly 
    1040                 1045                 1050             


Gln Thr  Gly Lys Ile Ala Asp  Tyr Asn Tyr Lys Leu  Pro Asp Asp 
    1055                 1060                 1065             


Phe Thr  Gly Cys Val Ile Ala  Trp Asn Ser Asn Asn  Leu Asp Ser 
    1070                 1075                 1080             


Lys Val  Gly Gly Asn Tyr Asn  Tyr Leu Tyr Arg Leu  Phe Arg Lys 
    1085                 1090                 1095             


Ser Asn  Leu Lys Pro Phe Glu  Arg Asp Ile Ser Thr  Glu Ile Tyr 
    1100                 1105                 1110             


Gln Ala  Gly Ser Thr Pro Cys  Asn Gly Val Glu Gly  Phe Asn Cys 
    1115                 1120                 1125             


Tyr Phe  Pro Leu Gln Ser Tyr  Gly Phe Gln Pro Thr  Asn Gly Val 
    1130                 1135                 1140             


Gly Tyr  Gln Pro Tyr Arg Val  Val Val Leu Ser Phe  Glu Leu Leu 
    1145                 1150                 1155             


His Ala  Pro Ala Thr Val Cys  Gly Pro Lys Lys Ser  Thr Asn Leu 
    1160                 1165                 1170             


Val Lys  Asn Lys Cys Val Asn  Phe 
    1175                 1180     


