                         SEQUENCE LISTING

<110>  JL BIOSCIENCES, INC.
       AIKENS, John
       CABALKA TOURTELOTTE, LeAnne M
 
<120>  ENZYMES ENTRAPPED IN ORGANOPOLYSILOXANE MATRIX

<130>  68446-212902

<140>  PCT/US2021/060583
<141>  2021-11-23

<150>  63/117,648
<151>  2020-11-24

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  332
<212>  PRT
<213>  Geobacillus stearothermophilus 10

<400>  1

Met Ala Lys Thr Val Glu Thr Val Leu Gly Pro Val Pro Val Glu Gln 
1               5                   10                  15      


Leu Gly Lys Thr Leu Ile His Glu His Phe Leu Phe Gly Tyr Pro Gly 
            20                  25                  30          


Phe Gln Gly Asp Val Thr Arg Gly Thr Phe Arg Glu Asp Glu Ala Leu 
        35                  40                  45              


Arg Val Ala Val Glu Ala Ala Glu Lys Met Lys Arg His Gly Ile Gln 
    50                  55                  60                  


Thr Val Val Asp Pro Thr Pro Asn Asp Cys Gly Arg Asn Pro Ala Phe 
65                  70                  75                  80  


Leu Arg Arg Val Ala Glu Glu Thr Gly Leu Asn Ile Ile Cys Ala Thr 
                85                  90                  95      


Gly Tyr Tyr Tyr Glu Gly Glu Gly Ala Pro Pro Tyr Phe Gln Phe Arg 
            100                 105                 110         


Arg Leu Leu Gly Thr Ala Glu Asp Asp Ile Tyr Asp Met Phe Met Ala 
        115                 120                 125             


Glu Leu Thr Glu Gly Ile Ala Asp Thr Gly Ile Lys Ala Gly Val Ile 
    130                 135                 140                 


Lys Leu Ala Ser Ser Lys Gly Arg Ile Thr Glu Tyr Glu Lys Met Phe 
145                 150                 155                 160 


Phe Arg Ala Ala Ala Arg Ala Gln Lys Glu Thr Gly Ala Val Ile Ile 
                165                 170                 175     


Thr His Thr Gln Glu Gly Thr Met Gly Pro Glu Gln Ala Ala Tyr Leu 
            180                 185                 190         


Leu Glu His Gly Ala Asp Pro Lys Lys Ile Val Ile Gly His Met Cys 
        195                 200                 205             


Gly Asn Thr Asp Pro Asp Tyr His Arg Lys Thr Leu Ala Tyr Gly Val 
    210                 215                 220                 


Tyr Ile Ala Phe Asp Arg Phe Gly Ile Gln Gly Met Val Gly Ala Pro 
225                 230                 235                 240 


Thr Asp Glu Glu Arg Val Arg Thr Leu Leu Ala Leu Leu Arg Asp Gly 
                245                 250                 255     


Tyr Glu Lys Gln Ile Met Leu Ser His Asp Thr Val Asn Val Trp Leu 
            260                 265                 270         


Gly Arg Pro Phe Thr Leu Pro Glu Pro Phe Ala Glu Met Met Lys Asn 
        275                 280                 285             


Trp His Val Glu His Leu Phe Val Asn Ile Ile Pro Ala Leu Lys Asn 
    290                 295                 300                 


Glu Gly Ile Arg Asp Glu Val Leu Glu Gln Met Phe Ile Gly Asn Pro 
305                 310                 315                 320 


Ala Ala Leu Phe Ser Ala His His His His His His 
                325                 330         


<210>  2
<211>  981
<212>  DNA
<213>  Geobacillus stearothermophilus 10

<400>  2
atggctaaaa ctgtcgaaac cgtccttggc ccagtgccgg tggaacagct tggcaaaacg       60

ctcatccacg agcatttcct cttcggctat ccagggtttc aaggcgatgt gacgcgcggc      120

acgttccgcg aagacgaggc gcttcgcgtc gcagtcgagg cggcggaaaa gatgaagcgg      180

cacggcattc aaacggttgt cgatcccacg ccgaacgact gcgggcgcaa cccggcattt      240

ttgcggcgcg ttgctgaaga gacgggactg aacattattt gcgccaccgg ctattattat      300

gaaggggaag gggcgccgcc gtacttccaa ttccgccggc ttctcggaac agcggaagat      360

gatatttacg acatgtttat ggccgagctg accgagggca ttgccgatac cggaatcaag      420

gcgggtgtca tcaagctcgc ctcgagcaaa gggcgcatca ccgagtacga aaagatgttc      480

ttccgcgctg ccgcccgcgc gcaaaaagag acgggtgcgg tcatcatcac ccatacgcaa      540

gaaggaacga tggggccgga acaagccgcc tatttgcttg agcacggcgc cgatccgaaa      600

aaaattgtca tcggccatat gtgcggcaac acggacccgg actatcatcg aaagacgctt      660

gcttacggcg tttacattgc gtttgaccgc ttcggcatcc aagggatggt cggcgcgccg      720

actgatgagg agcgggtgcg gacgctcctt gctctgctcc gcgatgggta cgagaaacaa      780

attatgctgt cgcatgacac tgtcaacgtt tggctcggtc gtccgtttac gctgccggaa      840

ccgtttgcgg aaatgatgaa aaattggcat gtcgagcatt tgtttgtgaa catcatcccc      900

gcgctgaaaa atgaaggaat ccgcgacgaa gtgcttgagc aaatgttcat cggcaatccg      960

gcggcgctgt tctcggcttg a                                                981


<210>  3
<211>  334
<212>  PRT
<213>  Geobacillus stearothermophilus F

<400>  3

Met Lys Lys Gly Met Val Glu Thr Val Cys Gly Pro Val Pro Ala Ser 
1               5                   10                  15      


Glu Leu Gly Lys Thr Leu Ile His Glu His Phe Val Phe Gly Tyr Pro 
            20                  25                  30          


Gly Phe Gln Gly Asp Val Thr Leu Gly Pro Leu Arg Phe Glu Glu Ala 
        35                  40                  45              


Leu Glu Ala Gly Ile Ala Val Ala Gln Lys Met Met Ala His Gly Ile 
    50                  55                  60                  


Gln Thr Val Val Asp Pro Thr Pro Asn Asp Cys Gly Arg Asn Pro Glu 
65                  70                  75                  80  


Leu Leu Arg Arg Ile Ser Glu Ala Thr Gly Leu Asn Ile Ile Cys Ala 
                85                  90                  95      


Thr Gly Tyr Tyr Tyr Glu Gly Glu Gly Ala Thr Pro Tyr Phe Lys Phe 
            100                 105                 110         


Arg Arg Leu Leu Gly Thr Ala Glu Glu Glu Ile Tyr Asp Met Phe Met 
        115                 120                 125             


Ala Glu Ile Thr Asn Gly Ile Ala Gly Thr Gly Ile Lys Pro Gly Val 
    130                 135                 140                 


Ile Lys Leu Ala Ser Ser Lys Asn Arg Ile Thr Glu Tyr Glu Lys Met 
145                 150                 155                 160 


Phe Phe Arg Ala Ala Ala Arg Val Gln Lys Glu Thr Gly Ile Val Ile 
                165                 170                 175     


Ile Thr His Thr Gln Glu Gly Thr Met Gly Pro Glu Gln Ala Ala Tyr 
            180                 185                 190         


Leu Leu Glu His Gly Ala Val Pro Gln Lys Ile Val Ile Gly His Met 
        195                 200                 205             


Cys Gly Asn Thr Asp Pro Glu Tyr His Lys Lys Thr Leu Gln Tyr Gly 
    210                 215                 220                 


Val Tyr Ile Ala Phe Asp Arg Phe Gly Leu Gln Gly Met Val Gly Ala 
225                 230                 235                 240 


Pro Thr Asp Glu Glu Arg Ile Arg Thr Leu Leu Val Leu Leu Arg Glu 
                245                 250                 255     


Gly Phe Ala Asp Lys Ile Met Leu Ala His Asp Thr Val Asn Ile Trp 
            260                 265                 270         


Leu Gly Arg Pro Leu Gln Leu Pro Glu Pro Phe Ala Thr Met Thr Lys 
        275                 280                 285             


Asn Trp His Val Glu His Val Phe Glu His Ile Ile Pro Ile Leu Thr 
    290                 295                 300                 


Lys Glu Gly Val Thr Glu Glu Gln Leu Glu Gln Met Phe Leu Lys Asn 
305                 310                 315                 320 


Pro Ala Ala Leu Phe Ala Ser Gly His His His His His His 
                325                 330                 


<210>  4
<211>  978
<212>  DNA
<213>  Geobacillus stearothermophilus F

<400>  4
atgaaaaaag gaatggtgga gactgtctgc ggccctgtgc cggcgagcga gcttggcaaa       60

acgctgatcc atgagcattt tgtctttggg tatcccgggt ttcaaggcga tgtgacgctc      120

ggaccgcttc gctttgagga agcgctcgag gcgggaattg ccgtcgcgca aaaaatgatg      180

gcccatggca tccaaaccgt tgtcgatccg acgccgaatg actgcggccg caatccggag      240

ctgctccgcc gcatttccga agcgaccggg ctgaacatta tctgtgcaac tggatattat      300

tacgaaggcg aaggggcaac gccgtatttt aagtttcgcc ggctgctcgg tacggcggaa      360

gaagaaatat atgacatgtt tatggcggaa attacaaacg gcattgccgg caccggcatt      420

aaaccaggcg tcatcaaact ggcgtcaagc aaaaaccgga tcaccgaata tgaaaaaatg      480

tttttccgcg ccgcggcccg cgtgcaaaaa gaaacaggaa tcgtgattat cacccatacg      540

caggaaggaa cgatggggcc ggaacaggcg gcatacttgc tcgagcacgg tgccgttccg      600

caaaagattg tcatcggcca tatgtgcgga aacaccgatc cggaatacca taaaaagacg      660

ctgcaatacg gagtgtacat cgcttttgac cgctttggcc ttcaaggtat ggtcggtgcc      720

ccgacggatg aagaacggat ccgcacgttg ctcgtgctgc ttcgcgaagg ctttgctgat      780

aagattatgc tcgctcatga taccgtcaat atttggctcg gccgtccgct gcagctgcca      840

gagccatttg cgacgatgac gaaaaactgg catgtcgaac atgtatttga acatattatt      900

ccaattttaa cgaaagaagg agtgacagag gaacagctag agcaaatgtt tttgaaaaat      960

cctgccgctt tgtttgcg                                                    978


<210>  5
<211>  314
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  5

Met Arg Ile Pro Leu Val Gly Lys Asp Ser Ile Glu Ser Lys Asp Ile 
1               5                   10                  15      


Gly Phe Thr Leu Ile His Glu His Leu Arg Val Phe Ser Glu Ala Val 
            20                  25                  30          


Arg Gln Gln Trp Pro His Leu Tyr Asn Glu Asp Glu Glu Phe Arg Asn 
        35                  40                  45              


Ala Val Asn Glu Val Lys Arg Ala Met Gln Phe Gly Val Lys Thr Ile 
    50                  55                  60                  


Val Asp Pro Thr Val Met Gly Leu Gly Arg Asp Ile Arg Phe Met Glu 
65                  70                  75                  80  


Lys Val Val Lys Ala Thr Gly Ile Asn Leu Val Ala Gly Thr Gly Ile 
                85                  90                  95      


Tyr Ile Tyr Ile Asp Leu Pro Phe Tyr Phe Leu Asn Arg Ser Ile Asp 
            100                 105                 110         


Glu Ile Ala Asp Leu Phe Ile His Asp Ile Lys Glu Gly Ile Gln Gly 
        115                 120                 125             


Thr Leu Asn Lys Ala Gly Phe Val Lys Ile Ala Ala Asp Glu Pro Gly 
    130                 135                 140                 


Ile Thr Lys Asp Val Glu Lys Val Ile Arg Ala Ala Ala Ile Ala Asn 
145                 150                 155                 160 


Lys Glu Thr Lys Val Pro Ile Ile Thr His Ser Asn Ala His Asn Asn 
                165                 170                 175     


Thr Gly Leu Glu Gln Gln Arg Ile Leu Thr Glu Glu Gly Val Asp Pro 
            180                 185                 190         


Gly Lys Ile Leu Ile Gly His Leu Gly Asp Thr Asp Asn Ile Asp Tyr 
        195                 200                 205             


Ile Lys Lys Ile Ala Asp Lys Gly Ser Phe Ile Gly Leu Asp Arg Tyr 
    210                 215                 220                 


Gly Leu Asp Leu Phe Leu Pro Val Asp Lys Arg Asn Glu Thr Thr Leu 
225                 230                 235                 240 


Arg Leu Ile Lys Asp Gly Tyr Ser Asp Lys Ile Met Ile Ser His Asp 
                245                 250                 255     


Tyr Cys Cys Thr Ile Asp Trp Gly Thr Ala Lys Pro Glu Tyr Lys Pro 
            260                 265                 270         


Lys Leu Ala Pro Arg Trp Ser Ile Thr Leu Ile Phe Glu Asp Thr Ile 
        275                 280                 285             


Pro Phe Leu Lys Arg Asn Gly Val Asn Glu Glu Val Ile Ala Thr Ile 
    290                 295                 300                 


Phe Lys Glu Asn Pro Lys Lys Phe Phe Ser 
305                 310                 


<210>  6
<211>  945
<212>  DNA
<213>  Sulfolobus solfataricus

<400>  6
atgagaatac cattagttgg gaaagattca atagaatcta aggacatagg atttacgcta       60

attcatgaac atttaagagt ttttagcgaa gcggtcagac aacaatggcc ccatctatat      120

aacgaagatg aggagttcag aaacgctgta aatgaggtta aaagggcaat gcaatttgga      180

gtaaagacta tagtagatcc cactgtaatg ggattgggta gggacatcag atttatggaa      240

aaagtggtta aggctaccgg gataaattta gttgcgggga cggggattta catatatatc      300

gacttacctt tctatttctt aaataggtca attgatgaga tagctgactt gtttattcat      360

gatataaaag agggaataca aggtactctc aataaagctg gcttcgtaaa gatagctgca      420

gatgaacctg ggatcacaaa ggatgtggag aaggtaataa gggctgctgc catagcaaac      480

aaagagacta aagtaccaat aattacccac tctaacgctc acaataacac cggattagaa      540

cagcaaagaa tattgactga agaaggtgtt gatccaggga aaatattaat aggtcattta      600

ggtgatacag ataatataga ttacataaag aagatagcag ataagggatc ctttattgga      660

ttagatagat atggtttaga tttattccta cctgttgata agagaaatga aacgacctta      720

agactaatca aagatggtta ttcagataag ataatgatct ctcacgatta ttgttgcaca      780

atcgactggg gaactgcaaa accagaatat aaacctaagc ttgctccaag atggagtata      840

actctaatat ttgaggatac gataccgttc ttaaagagaa atggagtgaa tgaagaggtt      900

atagctacaa tatttaagga aaatccgaaa aagttcttca gctaa                      945


<210>  7
<211>  314
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  7

Met Arg Ile Pro Leu Val Gly Lys Asp Ser Ile Glu Ser Lys Asp Ile 
1               5                   10                  15      


Gly Phe Thr Leu Ile His Glu His Leu Arg Val Phe Ser Glu Ala Val 
            20                  25                  30          


Arg Gln Gln Trp Pro His Leu Tyr Asn Glu Asp Glu Glu Phe Arg Asn 
        35                  40                  45              


Ala Val Asn Glu Val Lys Arg Ala Met Gln Phe Gly Val Lys Thr Ile 
    50                  55                  60                  


Val Asp Pro Thr Val Met Gly Leu Gly Arg Asp Ile Arg Phe Met Glu 
65                  70                  75                  80  


Lys Val Val Lys Ala Thr Gly Ile Asn Leu Val Ala Gly Thr Gly Ile 
                85                  90                  95      


Tyr Ile Tyr Ile Asp Leu Pro Phe Tyr Phe Leu Asn Arg Ser Ile Asp 
            100                 105                 110         


Glu Ile Ala Asp Leu Phe Ile His Asp Ile Lys Glu Gly Ile Gln Gly 
        115                 120                 125             


Thr Leu Asn Lys Ala Gly Phe Val Lys Ile Ala Ala Asp Glu Pro Gly 
    130                 135                 140                 


Ile Thr Lys Asp Val Glu Lys Val Ile Arg Ala Ala Ala Ile Ala Asn 
145                 150                 155                 160 


Lys Glu Thr Lys Val Pro Ile Ile Thr His Ser Asn Ala His Asn Asn 
                165                 170                 175     


Thr Gly Leu Glu Gln Gln Arg Ile Leu Thr Glu Glu Gly Val Asp Pro 
            180                 185                 190         


Gly Lys Ile Leu Ile Gly His Leu Gly Asp Thr Asp Asn Ile Asp Tyr 
        195                 200                 205             


Ile Lys Lys Ile Ala Asp Lys Gly Ser Phe Ile Gly Leu Asp Arg Tyr 
    210                 215                 220                 


Gly Leu Asp Leu Phe Leu Pro Val Asp Lys Arg Asn Glu Thr Thr Leu 
225                 230                 235                 240 


Arg Leu Ile Lys Asp Gly Tyr Ser Asp Lys Ile Met Ile Ser His Asp 
                245                 250                 255     


Tyr Cys Cys Thr Ile Asp Ile Gly Thr Ala Lys Pro Glu Tyr Lys Pro 
            260                 265                 270         


Lys Leu Ala Pro Arg Trp Ser Ile Thr Leu Ile Phe Glu Asp Thr Ile 
        275                 280                 285             


Pro Phe Leu Lys Arg Asn Gly Val Asn Glu Glu Val Ile Ala Thr Ile 
    290                 295                 300                 


Phe Lys Glu Asn Pro Lys Lys Phe Phe Ser 
305                 310                 


<210>  8
<211>  945
<212>  DNA
<213>  Sulfolobus solfataricus

<400>  8
atgagaatac cattagttgg gaaagattca atagaatcta aggacatagg atttacgcta       60

attcatgaac atttaagagt ttttagcgaa gcggtcagac aacaatggcc ccatctatat      120

aacgaagatg aggagttcag aaacgctgta aatgaggtta aaagggcaat gcaatttgga      180

gtaaagacta tagtagatcc cactgtaatg ggattgggta gggacatcag atttatggaa      240

aaagtggtta aggctaccgg gataaattta gttgcgggga cggggattta catatatatc      300

gacttacctt tctatttctt aaataggtca attgatgaga tagctgactt gtttattcat      360

gatataaaag agggaataca aggtactctc aataaagctg gcttcgtaaa gatagctgca      420

gatgaacctg ggatcacaaa ggatgtggag aaggtaataa gggctgctgc catagcaaac      480

aaagagacta aagtaccaat aattacccac tctaacgctc acaataacac cggattagaa      540

cagcaaagaa tattgactga agaaggtgtt gatccaggga aaatattaat aggtcattta      600

ggtgatacag ataatataga ttacataaag aagatagcag ataagggatc ctttattgga      660

ttagatagat atggtttaga tttattccta cctgttgata agagaaatga aacgacctta      720

agactaatca aagaatttta ttcagataag ataatgatct ctcacgatta ttgttgcaca      780

atcgactggg gaactgcaaa accagaatat aaacctaagc ttgctccaag atggagtata      840

actctaatat ttgaggatac gataccgttc ttaaagagaa atggagtgaa tgaagaggtt      900

atagctacaa tatttaagga aaatccgaaa aagttcttca gctaa                      945


