                         SEQUENCE LISTING

<110>  Industry-Academic Cooperation Foundation, Yonsei University
 
<120>  A composition for prime editing comprising trans-splicing 
       adeno-associated virus vector

<130>  PX067223PCT

<150>  KR 10-2020-0160446
<151>  2020-11-25

<150>  KR 10-2021-0157739
<151>  2021-11-16

<160>  29    

<170>  PatentIn version 3.2

<210>  1
<211>  2091
<212>  PRT
<213>  Artificial

<220>
<223>  prime editor(nCas9-linker-RT)

<400>  1

Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          


Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              


Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys 
    50                  55                  60                  


Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  


Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      


Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         


Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             


Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 


Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 


Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     


Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         


Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             


Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 


Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 


Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     


Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         


Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             


Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 


Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 


Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     


Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         


Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             


Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 


Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 


Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     


Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         


Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 


Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 


Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     


Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         


Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             


Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 


Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 


Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     


Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 


Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 


Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 


Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp Ala Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1100                 1105                 1110             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1145                 1150                 1155             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1160                 1165                 1170             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1175                 1180                 1185             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1190                 1195                 1200             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1205                 1210                 1215             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1220                 1225                 1230             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1235                 1240                 1245             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1250                 1255                 1260             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1265                 1270                 1275             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1280                 1285                 1290             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1295                 1300                 1305             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1325                 1330                 1335             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp Ser 
    1355                 1360                 1365             


Gly Gly  Ser Ser Gly Gly Ser  Ser Gly Ser Glu Thr  Pro Gly Thr 
    1370                 1375                 1380             


Ser Glu  Ser Ala Thr Pro Glu  Ser Ser Gly Gly Ser  Ser Gly Gly 
    1385                 1390                 1395             


Ser Ser  Thr Leu Asn Ile Glu  Asp Glu Tyr Arg Leu  His Glu Thr 
    1400                 1405                 1410             


Ser Lys  Glu Pro Asp Val Ser  Leu Gly Ser Thr Trp  Leu Ser Asp 
    1415                 1420                 1425             


Phe Pro  Gln Ala Trp Ala Glu  Thr Gly Gly Met Gly  Leu Ala Val 
    1430                 1435                 1440             


Arg Gln  Ala Pro Leu Ile Ile  Pro Leu Lys Ala Thr  Ser Thr Pro 
    1445                 1450                 1455             


Val Ser  Ile Lys Gln Tyr Pro  Met Ser Gln Glu Ala  Arg Leu Gly 
    1460                 1465                 1470             


Ile Lys  Pro His Ile Gln Arg  Leu Leu Asp Gln Gly  Ile Leu Val 
    1475                 1480                 1485             


Pro Cys  Gln Ser Pro Trp Asn  Thr Pro Leu Leu Pro  Val Lys Lys 
    1490                 1495                 1500             


Pro Gly  Thr Asn Asp Tyr Arg  Pro Val Gln Asp Leu  Arg Glu Val 
    1505                 1510                 1515             


Asn Lys  Arg Val Glu Asp Ile  His Pro Thr Val Pro  Asn Pro Tyr 
    1520                 1525                 1530             


Asn Leu  Leu Ser Gly Leu Pro  Pro Ser His Gln Trp  Tyr Thr Val 
    1535                 1540                 1545             


Leu Asp  Leu Lys Asp Ala Phe  Phe Cys Leu Arg Leu  His Pro Thr 
    1550                 1555                 1560             


Ser Gln  Pro Leu Phe Ala Phe  Glu Trp Arg Asp Pro  Glu Met Gly 
    1565                 1570                 1575             


Ile Ser  Gly Gln Leu Thr Trp  Thr Arg Leu Pro Gln  Gly Phe Lys 
    1580                 1585                 1590             


Asn Ser  Pro Thr Leu Phe Asn  Glu Ala Leu His Arg  Asp Leu Ala 
    1595                 1600                 1605             


Asp Phe  Arg Ile Gln His Pro  Asp Leu Ile Leu Leu  Gln Tyr Val 
    1610                 1615                 1620             


Asp Asp  Leu Leu Leu Ala Ala  Thr Ser Glu Leu Asp  Cys Gln Gln 
    1625                 1630                 1635             


Gly Thr  Arg Ala Leu Leu Gln  Thr Leu Gly Asn Leu  Gly Tyr Arg 
    1640                 1645                 1650             


Ala Ser  Ala Lys Lys Ala Gln  Ile Cys Gln Lys Gln  Val Lys Tyr 
    1655                 1660                 1665             


Leu Gly  Tyr Leu Leu Lys Glu  Gly Gln Arg Trp Leu  Thr Glu Ala 
    1670                 1675                 1680             


Arg Lys  Glu Thr Val Met Gly  Gln Pro Thr Pro Lys  Thr Pro Arg 
    1685                 1690                 1695             


Gln Leu  Arg Glu Phe Leu Gly  Lys Ala Gly Phe Cys  Arg Leu Phe 
    1700                 1705                 1710             


Ile Pro  Gly Phe Ala Glu Met  Ala Ala Pro Leu Tyr  Pro Leu Thr 
    1715                 1720                 1725             


Lys Pro  Gly Thr Leu Phe Asn  Trp Gly Pro Asp Gln  Gln Lys Ala 
    1730                 1735                 1740             


Tyr Gln  Glu Ile Lys Gln Ala  Leu Leu Thr Ala Pro  Ala Leu Gly 
    1745                 1750                 1755             


Leu Pro  Asp Leu Thr Lys Pro  Phe Glu Leu Phe Val  Asp Glu Lys 
    1760                 1765                 1770             


Gln Gly  Tyr Ala Lys Gly Val  Leu Thr Gln Lys Leu  Gly Pro Trp 
    1775                 1780                 1785             


Arg Arg  Pro Val Ala Tyr Leu  Ser Lys Lys Leu Asp  Pro Val Ala 
    1790                 1795                 1800             


Ala Gly  Trp Pro Pro Cys Leu  Arg Met Val Ala Ala  Ile Ala Val 
    1805                 1810                 1815             


Leu Thr  Lys Asp Ala Gly Lys  Leu Thr Met Gly Gln  Pro Leu Val 
    1820                 1825                 1830             


Ile Leu  Ala Pro His Ala Val  Glu Ala Leu Val Lys  Gln Pro Pro 
    1835                 1840                 1845             


Asp Arg  Trp Leu Ser Asn Ala  Arg Met Thr His Tyr  Gln Ala Leu 
    1850                 1855                 1860             


Leu Leu  Asp Thr Asp Arg Val  Gln Phe Gly Pro Val  Val Ala Leu 
    1865                 1870                 1875             


Asn Pro  Ala Thr Leu Leu Pro  Leu Pro Glu Glu Gly  Leu Gln His 
    1880                 1885                 1890             


Asn Cys  Leu Asp Ile Leu Ala  Glu Ala His Gly Thr  Arg Pro Asp 
    1895                 1900                 1905             


Leu Thr  Asp Gln Pro Leu Pro  Asp Ala Asp His Thr  Trp Tyr Thr 
    1910                 1915                 1920             


Asp Gly  Ser Ser Leu Leu Gln  Glu Gly Gln Arg Lys  Ala Gly Ala 
    1925                 1930                 1935             


Ala Val  Thr Thr Glu Thr Glu  Val Ile Trp Ala Lys  Ala Leu Pro 
    1940                 1945                 1950             


Ala Gly  Thr Ser Ala Gln Arg  Ala Glu Leu Ile Ala  Leu Thr Gln 
    1955                 1960                 1965             


Ala Leu  Lys Met Ala Glu Gly  Lys Lys Leu Asn Val  Tyr Thr Asp 
    1970                 1975                 1980             


Ser Arg  Tyr Ala Phe Ala Thr  Ala His Ile His Gly  Glu Ile Tyr 
    1985                 1990                 1995             


Arg Arg  Arg Gly Trp Leu Thr  Ser Glu Gly Lys Glu  Ile Lys Asn 
    2000                 2005                 2010             


Lys Asp  Glu Ile Leu Ala Leu  Leu Lys Ala Leu Phe  Leu Pro Lys 
    2015                 2020                 2025             


Arg Leu  Ser Ile Ile His Cys  Pro Gly His Gln Lys  Gly His Ser 
    2030                 2035                 2040             


Ala Glu  Ala Arg Gly Asn Arg  Met Ala Asp Gln Ala  Ala Arg Lys 
    2045                 2050                 2055             


Ala Ala  Ile Thr Glu Thr Pro  Asp Thr Ser Thr Leu  Leu Ile Glu 
    2060                 2065                 2070             


Asn Ser  Ser Pro Ser Gly Gly  Ser Lys Arg Thr Ala  Asp Gly Ser 
    2075                 2080                 2085             


Glu Phe  Glu 
    2090     


<210>  2
<211>  6273
<212>  DNA
<213>  Artificial

<220>
<223>  prime editor(nCas9-linker-RT)

<400>  2
gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc     60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac    120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc    180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat    240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg    300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac    360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa    420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg    480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg    540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc    600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg    660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg    720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat    780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag    840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg    900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg    960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag   1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc   1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa   1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag   1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc   1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag   1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga   1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg   1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac   1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat   1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc   1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg   1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc   1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc   1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg   1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac   1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg   1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat   2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc   2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac   2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg   2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc   2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg   2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg   2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat   2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc   2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac   2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac   2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc   2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg   2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact   2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag   2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac   2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac   3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg   3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac   3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct   3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc   3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag   3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc   3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat   3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa   3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt   3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac   3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag   3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac   3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag   3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc   3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc   3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct   3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag   4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac   4080

ctgtctcagc tgggaggtga ctctggagga tctagcggag gatcctctgg cagcgagaca   4140

ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcagc   4200

accctaaata tagaagatga gtatcggcta catgagacct caaaagagcc agatgtttct   4260

ctagggtcca catggctgtc tgattttcct caggcctggg cggaaaccgg gggcatggga   4320

ctggcagttc gccaagctcc tctgatcata cctctgaaag caacctctac ccccgtgtcc   4380

ataaaacaat accccatgtc acaagaagcc agactgggga tcaagcccca catacagaga   4440

ctgttggacc agggaatact ggtaccctgc cagtccccct ggaacacgcc cctgctaccc   4500

gttaagaaac cagggactaa tgattatagg cctgtccagg atctgagaga agtcaacaag   4560

cgggtggaag acatccaccc caccgtgccc aacccttaca acctcttgag cgggctccca   4620

ccgtcccacc agtggtacac tgtgcttgat ttaaaggatg cctttttctg cctgagactc   4680

caccccacca gtcagcctct cttcgccttt gagtggagag atccagagat gggaatctca   4740

ggacaattga cctggaccag actcccacag ggtttcaaaa acagtcccac cctgtttaat   4800

gaggcactgc acagagacct agcagacttc cggatccagc acccagactt gatcctgcta   4860

cagtacgtgg atgacttact gctggccgcc acttctgagc tagactgcca acaaggtact   4920

cgggccctgt tacaaaccct agggaacctc gggtatcggg cctcggccaa gaaagcccaa   4980

atttgccaga aacaggtcaa gtatctgggg tatcttctaa aagagggtca gagatggctg   5040

actgaggcca gaaaagagac tgtgatgggg cagcctactc cgaagacccc tcgacaacta   5100

agggagttcc tagggaaggc aggcttctgt cgcctcttca tccctgggtt tgcagaaatg   5160

gcagcccccc tgtaccctct caccaaaccg gggactctgt ttaattgggg cccagaccaa   5220

caaaaggcct atcaagaaat caagcaagct cttctaactg ccccagccct ggggttgcca   5280

gatttgacta agccctttga actctttgtc gacgagaagc agggctacgc caaaggtgtc   5340

ctaacgcaaa aactgggacc ttggcgtcgg ccggtggcct acctgtccaa aaagctagac   5400

ccagtagcag ctgggtggcc cccttgccta cggatggtag cagccattgc cgtactgaca   5460

aaggatgcag gcaagctaac catgggacag ccactagtca ttctggcccc ccatgcagta   5520

gaggcactag tcaaacaacc ccccgaccgc tggctttcca acgcccggat gactcactat   5580

caggccttgc ttttggacac ggaccgggtc cagttcggac cggtggtagc cctgaacccg   5640

gctacgctgc tcccactgcc tgaggaaggg ctgcaacaca actgccttga tatcctggcc   5700

gaagcccacg gaacccgacc cgacctaacg gaccagccgc tcccagacgc cgaccacacc   5760

tggtacacgg atggaagcag tctcttacaa gagggacagc gtaaggcggg agctgcggtg   5820

accaccgaga ccgaggtaat ctgggctaaa gccctgccag ccgggacatc cgctcagcgg   5880

gctgaactga tagcactcac ccaggcccta aagatggcag aaggtaagaa gctaaatgtt   5940

tatactgata gccgttatgc ttttgctact gcccatatcc atggagaaat atacagaagg   6000

cgtgggtggc tcacatcaga aggcaaagag atcaaaaata aagacgagat cttggcccta   6060

ctaaaagccc tctttctgcc caaaagactt agcataatcc attgtccagg acatcaaaag   6120

ggacacagcg ccgaggctag aggcaaccgg atggctgacc aagcggcccg aaaggcagcc   6180

atcacagaga ctccagacac ctctaccctc ctcatagaaa attcatcacc ctctggcggc   6240

tcaaaaagaa ccgccgacgg cagcgaattc gag                                6273


<210>  3
<211>  122
<212>  DNA
<213>  Artificial

<220>
<223>  pegRNA

<400>  3
gtgcctttca gtttctgggt gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt gggaccgagt cggtccagta ccacccatcc agaaactgaa    120

ag                                                                   122


<210>  4
<211>  96
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA

<400>  4
gatgagtacg tccatgttgg gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgc                               96


<210>  5
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-N_F1

<400>  5
gcagagctct ctggctaact accggtgcca ccatgaaacg gacag                     45


<210>  6
<211>  24
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-N_F2

<400>  6
gtaagtatca aggttacaag acag                                            24


<210>  7
<211>  42
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-N_R1

<400>  7
ctgtcttgta accttgatac ttacgtactt ggcggtagcc tt                        42


<210>  8
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-N_R2

<400>  8
catcactagg ggttcctgcg                                                 20


<210>  9
<211>  47
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_F1

<400>  9
tcactagggg ttcctgcggc ctctagactt gtcgagacag agaagac                   47


<210>  10
<211>  44
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_F2

<400>  10
ctttgccttt ctctccacag ttcttctaca gcaacatcat gaac                      44


<210>  11
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_F3

<400>  11
aatcaacctc tggattacaa aat                                             23


<210>  12
<211>  38
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_F4

<400>  12
tttgggccgc ctccccgcct gtgccttcta gttgccag                             38


<210>  13
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_R1

<400>  13
ctgtggagag aaaggcaaag                                                 20


<210>  14
<211>  45
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_R2

<400>  14
attttgtaat ccagaggttg atttggtgat gatgaccggt tagac                     45


<210>  15
<211>  18
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_R3

<400>  15
gcggggaggc ggcccaaa                                                   18


<210>  16
<211>  46
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE_R4

<400>  16
ctccatcact aggggttcct gcggccgccc atagagccca ccgcat                    46


<210>  17
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA1_input

<400>  17
aagcctacaa atcgctgaga cacaggtccg                                      30


<210>  18
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA1_target

<400>  18
cggacctgtg tctcagcgat ttgtaggctt                                      30


<210>  19
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA1_guide

<400>  19
cctgtgtctc agcgatttgt                                                 20


<210>  20
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA2_input

<400>  20
ggtccgccaa catggacgta ctcatcgtgc                                      30


<210>  21
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA2_target

<400>  21
gcacgatgag tacgtccatg ttggcggacc                                      30


<210>  22
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA2_guide

<400>  22
gatgagtacg tccatgttgg                                                 20


<210>  23
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA3_input

<400>  23
ccgccaacat ggacgtactc atcgtgctcg                                      30


<210>  24
<211>  30
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA3_target

<400>  24
cgagcacgat gagtacgtcc atgttggcgg                                      30


<210>  25
<211>  20
<212>  DNA
<213>  Artificial

<220>
<223>  sgRNA3_guide

<400>  25
cacgatgagt acgtccatgt                                                 20


<210>  26
<211>  4122
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-N

<400>  26
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt     60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact    120

aggggttcct gcggcctcta gactcgaggc gttgacattg attattgact agttattaat    180

agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac    240

ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa    300

tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt    360

atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc    420

ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat    480

gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc    540

ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc    600

tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa    660

aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg    720

tctatataag cagagctctc tggctaacta ccggtgccac catgaaacgg acagccgacg    780

gaagcgagtt cgagtcacca aagaagaagc ggaaagtcga caagaagtac agcatcggcc    840

tggacatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca    900

gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg    960

gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca   1020

gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg   1080

agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag   1140

aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc   1200

acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg   1260

ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc   1320

tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg   1380

tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc gtggacgcca   1440

aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg atcgcccagc   1500

tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc ctgggcctga   1560

cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg   1620

acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag tacgccgacc   1680

tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc ctgagagtga   1740

acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac gacgagcacc   1800

accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag aagtacaaag   1860

agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc ggagccagcc   1920

aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc accgaggaac   1980

tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc gacaacggca   2040

gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg caggaagatt   2100

tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc   2160

cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg accagaaaga   2220

gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc gcttccgccc   2280

agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag aaggtgctgc   2340

ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc aaagtgaaat   2400

acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg   2460

tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa gaggactact   2520

tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat cggttcaacg   2580

cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac ttcctggaca   2640

atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg tttgaggaca   2700

gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac aaagtgatga   2760

agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag ctgatcaacg   2820

gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc gacggcttcg   2880

ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa gaggacatcc   2940

agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc aatctggccg   3000

gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac gagctcgtga   3060

aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga gagaaccaga   3120

ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa gagggcatca   3180

aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag ctgcagaacg   3240

agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac caggaactgg   3300

acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc tttctgaagg   3360

acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc aagagcgaca   3420

acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg   3480

ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga ggcggcctga   3540

gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg cagatcacaa   3600

agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag aatgacaagc   3660

tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat ttccggaagg   3720

atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac gacgcctacc   3780

tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa agcgagttcg   3840

tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa   3900

tcggcaaggc taccgccaag tacgtaagta tcaaggttac aagacaggtt taaggagacc   3960

aatagaaact ggggcggccg caggaacccc tagtgatgga gttggccact ccctctctgc   4020

gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc   4080

gggcggcctc agtgagcgag cgagcgcgca gctgcctgca gg                      4122


<210>  27
<211>  4396
<212>  DNA
<213>  Artificial

<220>
<223>  TS-PE2-C-WPRE

<400>  27
cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt     60

ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact    120

aggggttcct gcggcctcta gacttgtcga gacagagaag actcttgcgt ttctgatagg    180

cacctattgg tcttactgac atccactttg cctttctctc cacagttctt ctacagcaac    240

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct    300

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc    360

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag    420

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc    480

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat    540

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa    600

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt    660

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac    720

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag    780

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac    840

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag    900

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc    960

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc   1020

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct   1080

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag   1140

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac   1200

ctgtctcagc tgggaggtga ctctggagga tctagcggag gatcctctgg cagcgagaca   1260

ccaggaacaa gcgagtcagc aacaccagag agcagtggcg gcagcagcgg cggcagcagc   1320

accctaaata tagaagatga gtatcggcta catgagacct caaaagagcc agatgtttct   1380

ctagggtcca catggctgtc tgattttcct caggcctggg cggaaaccgg gggcatggga   1440

ctggcagttc gccaagctcc tctgatcata cctctgaaag caacctctac ccccgtgtcc   1500

ataaaacaat accccatgtc acaagaagcc agactgggga tcaagcccca catacagaga   1560

ctgttggacc agggaatact ggtaccctgc cagtccccct ggaacacgcc cctgctaccc   1620

gttaagaaac cagggactaa tgattatagg cctgtccagg atctgagaga agtcaacaag   1680

cgggtggaag acatccaccc caccgtgccc aacccttaca acctcttgag cgggctccca   1740

ccgtcccacc agtggtacac tgtgcttgat ttaaaggatg cctttttctg cctgagactc   1800

caccccacca gtcagcctct cttcgccttt gagtggagag atccagagat gggaatctca   1860

ggacaattga cctggaccag actcccacag ggtttcaaaa acagtcccac cctgtttaat   1920

gaggcactgc acagagacct agcagacttc cggatccagc acccagactt gatcctgcta   1980

cagtacgtgg atgacttact gctggccgcc acttctgagc tagactgcca acaaggtact   2040

cgggccctgt tacaaaccct agggaacctc gggtatcggg cctcggccaa gaaagcccaa   2100

atttgccaga aacaggtcaa gtatctgggg tatcttctaa aagagggtca gagatggctg   2160

actgaggcca gaaaagagac tgtgatgggg cagcctactc cgaagacccc tcgacaacta   2220

agggagttcc tagggaaggc aggcttctgt cgcctcttca tccctgggtt tgcagaaatg   2280

gcagcccccc tgtaccctct caccaaaccg gggactctgt ttaattgggg cccagaccaa   2340

caaaaggcct atcaagaaat caagcaagct cttctaactg ccccagccct ggggttgcca   2400

gatttgacta agccctttga actctttgtc gacgagaagc agggctacgc caaaggtgtc   2460

ctaacgcaaa aactgggacc ttggcgtcgg ccggtggcct acctgtccaa aaagctagac   2520

ccagtagcag ctgggtggcc cccttgccta cggatggtag cagccattgc cgtactgaca   2580

aaggatgcag gcaagctaac catgggacag ccactagtca ttctggcccc ccatgcagta   2640

gaggcactag tcaaacaacc ccccgaccgc tggctttcca acgcccggat gactcactat   2700

caggccttgc ttttggacac ggaccgggtc cagttcggac cggtggtagc cctgaacccg   2760

gctacgctgc tcccactgcc tgaggaaggg ctgcaacaca actgccttga tatcctggcc   2820

gaagcccacg gaacccgacc cgacctaacg gaccagccgc tcccagacgc cgaccacacc   2880

tggtacacgg atggaagcag tctcttacaa gagggacagc gtaaggcggg agctgcggtg   2940

accaccgaga ccgaggtaat ctgggctaaa gccctgccag ccgggacatc cgctcagcgg   3000

gctgaactga tagcactcac ccaggcccta aagatggcag aaggtaagaa gctaaatgtt   3060

tatactgata gccgttatgc ttttgctact gcccatatcc atggagaaat atacagaagg   3120

cgtgggtggc tcacatcaga aggcaaagag atcaaaaata aagacgagat cttggcccta   3180

ctaaaagccc tctttctgcc caaaagactt agcataatcc attgtccagg acatcaaaag   3240

ggacacagcg ccgaggctag aggcaaccgg atggctgacc aagcggcccg aaaggcagcc   3300

atcacagaga ctccagacac ctctaccctc ctcatagaaa attcatcacc ctctggcggc   3360

tcaaaaagaa ccgccgacgg cagcgaattc gagcccaaga agaagaggaa agtctaaccg   3420

gtcatcatca ccaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct   3480

taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc   3540

tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct   3600

ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga   3660

cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc   3720

tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac   3780

aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt   3840

tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt   3900

cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc   3960

tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc   4020

gcctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac   4080

cctggaaggt gccactccca ctgtcctttc ctaataaaat gagaaaattg catcgcattg   4140

tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga   4200

ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgggcg gccgcaggaa   4260

cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg   4320

cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg   4380

cgcagctgcc tgcagg                                                   4396


<210>  28
<211>  118
<212>  DNA
<213>  Artificial

<220>
<223>  pegRNA 157

<400>  28
ggagccctgg cccacatcag gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgcag tctcctccga tgtgggcc      118


<210>  29
<211>  119
<212>  DNA
<213>  Artificial

<220>
<223>  pegRNA 198

<400>  29
ggagccctgg cccacatcag gttttagagc tagaaatagc aagttaaaat aaggctagtc     60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgcggca gtctcctccg atgtgggcc     119


