               SEQUENCE LISTING

<110> Janssen Pharmaceuticals, Inc.

<120> ANALYTICAL METHOD FOR GLYCOCONJUGATES USING A CAPILLARY-BASED IMMUNOASSAY SYSTEM

<130> CRU6040EPEPA1

<150> EP20210768.6
<151> 2020-11-30

<160> 3

<170> BiSSAP 1.3.6

<210> 1
<400> 1
000

<210> 2
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> optimized glycosylation consensus sequence

<220> 
<221> VARIANT
<222> 1
<223> Asp or Glu

<220> 
<221> VARIANT
<222> 2
<223> any amino acid residue except Pro

<220> 
<221> VARIANT
<222> 4
<223> any amino acid residue except Pro

<220> 
<221> VARIANT
<222> 5
<223> Ser or Thr

<400> 2
Xaa Xaa Asn Xaa Xaa 
1               5   

<210> 3
<211> 652
<212> PRT
<213> Artificial Sequence


<220> 
<223> EPA carrier protein including 4 N-linked glycosylation consensus
      sequences

<400> 3
Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr Gly Ser Gly Gly Gly Lys 
1               5                   10                  15      
Leu Ala Glu Glu Ala Phe Asp Leu Trp Asn Glu Cys Ala Lys Ala Cys 
            20                  25                  30          
Val Leu Asp Leu Lys Asp Gly Val Arg Ser Ser Arg Met Ser Val Asp 
        35                  40                  45              
Pro Ala Ile Ala Asp Thr Asn Gly Gln Gly Val Leu His Tyr Ser Met 
    50                  55                  60                  
Val Leu Glu Gly Gly Asn Asp Ala Leu Lys Leu Ala Ile Asp Asn Ala 
65                  70                  75                  80  
Leu Ser Ile Thr Ser Asp Gly Leu Thr Ile Arg Leu Glu Gly Gly Val 
                85                  90                  95      
Glu Pro Asn Lys Pro Val Arg Tyr Ser Tyr Thr Arg Gln Ala Arg Gly 
            100                 105                 110         
Ser Trp Ser Leu Asn Trp Leu Val Pro Ile Gly His Glu Lys Pro Ser 
        115                 120                 125             
Asn Ile Lys Val Phe Ile His Glu Leu Asn Ala Gly Asn Gln Leu Ser 
    130                 135                 140                 
His Met Ser Pro Ile Tyr Thr Ile Glu Met Gly Asp Glu Leu Leu Ala 
145                 150                 155                 160 
Lys Leu Ala Arg Asp Ala Thr Phe Phe Val Arg Ala His Glu Ser Asn 
                165                 170                 175     
Glu Met Gln Pro Thr Leu Ala Ile Ser His Ala Gly Val Ser Val Val 
            180                 185                 190         
Met Ala Gln Ala Gln Pro Arg Arg Glu Lys Arg Trp Ser Glu Trp Ala 
        195                 200                 205             
Ser Gly Lys Val Leu Cys Leu Leu Asp Pro Leu Asp Gly Val Tyr Asn 
    210                 215                 220                 
Tyr Leu Ala Gln Gln Arg Cys Asn Leu Asp Asp Thr Trp Glu Gly Lys 
225                 230                 235                 240 
Ile Tyr Arg Val Leu Ala Gly Asn Pro Ala Lys His Asp Leu Asp Ile 
                245                 250                 255     
Lys Asp Asn Asn Asn Ser Thr Pro Thr Val Ile Ser His Arg Leu His 
            260                 265                 270         
Phe Pro Glu Gly Gly Ser Leu Ala Ala Leu Thr Ala His Gln Ala Cys 
        275                 280                 285             
His Leu Pro Leu Glu Ala Phe Thr Arg His Arg Gln Pro Arg Gly Trp 
    290                 295                 300                 
Glu Gln Leu Glu Gln Cys Gly Tyr Pro Val Gln Arg Leu Val Ala Leu 
305                 310                 315                 320 
Tyr Leu Ala Ala Arg Leu Ser Trp Asn Gln Val Asp Gln Val Ile Arg 
                325                 330                 335     
Asn Ala Leu Ala Ser Pro Gly Ser Gly Gly Asp Leu Gly Glu Ala Ile 
            340                 345                 350         
Arg Glu Gln Pro Glu Gln Ala Arg Leu Ala Leu Thr Leu Ala Ala Ala 
        355                 360                 365             
Glu Ser Glu Arg Phe Val Arg Gln Gly Thr Gly Asn Asp Glu Ala Gly 
    370                 375                 380                 
Ala Ala Ser Ala Asp Val Val Ser Leu Thr Cys Pro Val Ala Lys Asp 
385                 390                 395                 400 
Gln Asn Arg Thr Lys Gly Glu Cys Ala Gly Pro Ala Asp Ser Gly Asp 
                405                 410                 415     
Ala Leu Leu Glu Arg Asn Tyr Pro Thr Gly Ala Glu Phe Leu Gly Asp 
            420                 425                 430         
Gly Gly Asp Val Ser Phe Ser Thr Arg Gly Thr Gln Asn Trp Thr Val 
        435                 440                 445             
Glu Arg Leu Leu Gln Ala His Arg Gln Leu Glu Glu Arg Gly Tyr Val 
    450                 455                 460                 
Phe Val Gly Tyr His Gly Thr Phe Leu Glu Ala Ala Gln Ser Ile Val 
465                 470                 475                 480 
Phe Gly Gly Val Arg Ala Arg Ser Gln Asp Leu Asp Ala Ile Trp Arg 
                485                 490                 495     
Gly Phe Tyr Ile Ala Gly Asp Pro Ala Leu Ala Tyr Gly Tyr Ala Gln 
            500                 505                 510         
Asp Gln Glu Pro Asp Ala Arg Gly Arg Ile Arg Asn Gly Ala Leu Leu 
        515                 520                 525             
Arg Val Tyr Val Pro Arg Trp Ser Leu Pro Gly Phe Tyr Arg Thr Gly 
    530                 535                 540                 
Leu Thr Leu Ala Ala Pro Glu Ala Ala Gly Glu Val Glu Arg Leu Ile 
545                 550                 555                 560 
Gly His Pro Leu Pro Leu Arg Leu Asp Ala Ile Thr Gly Pro Glu Glu 
                565                 570                 575     
Glu Gly Gly Arg Val Thr Ile Leu Gly Trp Pro Leu Ala Glu Arg Thr 
            580                 585                 590         
Val Val Ile Pro Ser Ala Ile Pro Thr Asp Pro Arg Asn Val Gly Gly 
        595                 600                 605             
Asp Leu Asp Pro Ser Ser Ile Pro Asp Lys Glu Gln Ala Ile Ser Ala 
    610                 615                 620                 
Leu Pro Asp Tyr Ala Ser Gln Pro Gly Lys Pro Pro Arg Glu Asp Leu 
625                 630                 635                 640 
Lys Leu Gly Ser Gly Gly Gly Asp Gln Asn Ala Thr 
                645                 650         

