                         LISTADO DE SECUENCIAS

<110>  Consejo Superior de Investigaciones Cientificas (CSIC)
 
<120>  Saccharomyces cerevisiae recombinante para la produccin de 
       hidroxitirosol

<130>  PCT1641.1569

<150>  ES P202031186
<151>  2020-11-26

<160>  58    

<170>  PatentIn version 3.5

<210>  1
<211>  1563
<212>  DNA
<213>  Escherichia coli

<400>  1
atgaaaccag aagatttccg cgccagtacc caacgtccgt tcaccgggga agagtatctg       60

aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa agacgtcact      120

actcatccgg catttcgtaa tgcggctgcg tctgttgccc aactgtacga cgcgctacac      180

aaaccggaga tgcaggactc tctgtgctgg aacaccgaca ccggcagcgg cggctatacc      240

cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccacgaacg cgatgccatc      300

gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta caaagctgct      360

ttcggttgcg cactgggcgg aactccgggc ttttacggtc agttcgagca gaacgcccgt      420

aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat tgttaaccca      480

ccgatcgatc gtcatttgcc gaccgataaa gtaaaagacg tttacatcaa gctggaaaaa      540

gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa ctcggcgctg      600

actcactaca acatgattgg cttcggctcg gcacaagtaa tgggcgaaaa cccggacttc      660

gcactgatgt tcgttgcgcc aatggatgcc gatggcgtca aattaatctc ccgcgcctct      720

tatgagatgg tcgcgggtgc taccggctca ccgtatgact acccgctctc cagccgcttc      780

gatgagaacg atgcgattct ggtgatggat aacgtgctga tcccatggga aaacgtgctg      840

ctctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt cgcccgtatg      900

tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg acttcattac ggcactgctg      960

aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc cgatctcggt     1020

gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg actcgatgtg ttctgaagcg     1080

acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac ctatcgcgta     1140

ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt taccagtggc     1200

ctgatctatc tcccttccag tgcccgtgac ctgaacaatc cgcagatcga ccagtatctg     1260

gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa gatcctcaaa     1320

ctgatgtggg atgccattgg cagcgagttt ggtggtcgtc acgaactgta tgaaatcaac     1380

tactccggta gccaggatga gattcgcctg cagtgtctgc gccaggcaca aagctccggc     1440

aatatggaca agatgatggc gatggttgat cgctgcctgt cggaatacga ccagaacggc     1500

tggactgtgc cgcacctgca caacaacgac gatatcaaca tgctggataa gctgctgaaa     1560

taa                                                                   1563


<210>  2
<211>  520
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Lys Pro Glu Asp Phe Arg Ala Ser Thr Gln Arg Pro Phe Thr Gly 
1               5                   10                  15      


Glu Glu Tyr Leu Lys Ser Leu Gln Asp Gly Arg Glu Ile Tyr Ile Tyr 
            20                  25                  30          


Gly Glu Arg Val Lys Asp Val Thr Thr His Pro Ala Phe Arg Asn Ala 
        35                  40                  45              


Ala Ala Ser Val Ala Gln Leu Tyr Asp Ala Leu His Lys Pro Glu Met 
    50                  55                  60                  


Gln Asp Ser Leu Cys Trp Asn Thr Asp Thr Gly Ser Gly Gly Tyr Thr 
65                  70                  75                  80  


His Lys Phe Phe Arg Val Ala Lys Ser Ala Asp Asp Leu Arg His Glu 
                85                  90                  95      


Arg Asp Ala Ile Ala Glu Trp Ser Arg Leu Ser Tyr Gly Trp Met Gly 
            100                 105                 110         


Arg Thr Pro Asp Tyr Lys Ala Ala Phe Gly Cys Ala Leu Gly Gly Thr 
        115                 120                 125             


Pro Gly Phe Tyr Gly Gln Phe Glu Gln Asn Ala Arg Asn Trp Tyr Thr 
    130                 135                 140                 


Arg Ile Gln Glu Thr Gly Leu Tyr Phe Asn His Ala Ile Val Asn Pro 
145                 150                 155                 160 


Pro Ile Asp Arg His Leu Pro Thr Asp Lys Val Lys Asp Val Tyr Ile 
                165                 170                 175     


Lys Leu Glu Lys Glu Thr Asp Ala Gly Ile Ile Val Ser Gly Ala Lys 
            180                 185                 190         


Val Val Ala Thr Asn Ser Ala Leu Thr His Tyr Asn Met Ile Gly Phe 
        195                 200                 205             


Gly Ser Ala Gln Val Met Gly Glu Asn Pro Asp Phe Ala Leu Met Phe 
    210                 215                 220                 


Val Ala Pro Met Asp Ala Asp Gly Val Lys Leu Ile Ser Arg Ala Ser 
225                 230                 235                 240 


Tyr Glu Met Val Ala Gly Ala Thr Gly Ser Pro Tyr Asp Tyr Pro Leu 
                245                 250                 255     


Ser Ser Arg Phe Asp Glu Asn Asp Ala Ile Leu Val Met Asp Asn Val 
            260                 265                 270         


Leu Ile Pro Trp Glu Asn Val Leu Leu Tyr Arg Asp Phe Asp Arg Cys 
        275                 280                 285             


Arg Arg Trp Thr Met Glu Gly Gly Phe Ala Arg Met Tyr Pro Leu Gln 
    290                 295                 300                 


Ala Cys Val Arg Leu Ala Val Lys Leu Asp Phe Ile Thr Ala Leu Leu 
305                 310                 315                 320 


Lys Lys Ser Leu Glu Cys Thr Gly Thr Leu Glu Phe Arg Gly Val Gln 
                325                 330                 335     


Ala Asp Leu Gly Glu Val Val Ala Trp Arg Asn Thr Phe Trp Ala Leu 
            340                 345                 350         


Ser Asp Ser Met Cys Ser Glu Ala Thr Pro Trp Val Asn Gly Ala Tyr 
        355                 360                 365             


Leu Pro Asp His Ala Ala Leu Gln Thr Tyr Arg Val Leu Ala Pro Met 
    370                 375                 380                 


Ala Tyr Ala Lys Ile Lys Asn Ile Ile Glu Arg Asn Val Thr Ser Gly 
385                 390                 395                 400 


Leu Ile Tyr Leu Pro Ser Ser Ala Arg Asp Leu Asn Asn Pro Gln Ile 
                405                 410                 415     


Asp Gln Tyr Leu Ala Lys Tyr Val Arg Gly Ser Asn Gly Met Asp His 
            420                 425                 430         


Val Gln Arg Ile Lys Ile Leu Lys Leu Met Trp Asp Ala Ile Gly Ser 
        435                 440                 445             


Glu Phe Gly Gly Arg His Glu Leu Tyr Glu Ile Asn Tyr Ser Gly Ser 
    450                 455                 460                 


Gln Asp Glu Ile Arg Leu Gln Cys Leu Arg Gln Ala Gln Ser Ser Gly 
465                 470                 475                 480 


Asn Met Asp Lys Met Met Ala Met Val Asp Arg Cys Leu Ser Glu Tyr 
                485                 490                 495     


Asp Gln Asn Gly Trp Thr Val Pro His Leu His Asn Asn Asp Asp Ile 
            500                 505                 510         


Asn Met Leu Asp Lys Leu Leu Lys 
        515                 520 


<210>  3
<211>  513
<212>  DNA
<213>  Escherichia coli

<400>  3
atgcaattag atgaacaacg cctgcgcttt cgtgacgcaa tggccagcct gtcggcagcg       60

gtaaatatta tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc      120

tgctcggtca cggatacacc accatcgctg atggtgtgca ttaacgccaa cagtgcgatg      180

aacccggttt ttcagggcaa cggtaagttg tgcgtcaacg tcctcaacca tgagcaggaa      240

ctgatggcac gccacttcgc gggcatgaca ggcatggcga tggaagagcg ttttagcctc      300

tcatgctggc aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt      360

gaaggtgaga tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt      420

aaaaacatca tcctcagtgc agaaggtcac ggacttatct actttaaacg ccgtttccat      480

ccggtgatgc tggaaatgga agctgcgatt taa                                   513


<210>  4
<211>  170
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Gln Leu Asp Glu Gln Arg Leu Arg Phe Arg Asp Ala Met Ala Ser 
1               5                   10                  15      


Leu Ser Ala Ala Val Asn Ile Ile Thr Thr Glu Gly Asp Ala Gly Gln 
            20                  25                  30          


Cys Gly Ile Thr Ala Thr Ala Val Cys Ser Val Thr Asp Thr Pro Pro 
        35                  40                  45              


Ser Leu Met Val Cys Ile Asn Ala Asn Ser Ala Met Asn Pro Val Phe 
    50                  55                  60                  


Gln Gly Asn Gly Lys Leu Cys Val Asn Val Leu Asn His Glu Gln Glu 
65                  70                  75                  80  


Leu Met Ala Arg His Phe Ala Gly Met Thr Gly Met Ala Met Glu Glu 
                85                  90                  95      


Arg Phe Ser Leu Ser Cys Trp Gln Lys Gly Pro Leu Ala Gln Pro Val 
            100                 105                 110         


Leu Lys Gly Ser Leu Ala Ser Leu Glu Gly Glu Ile Arg Asp Val Gln 
        115                 120                 125             


Ala Ile Gly Thr His Leu Val Tyr Leu Val Glu Ile Lys Asn Ile Ile 
    130                 135                 140                 


Leu Ser Ala Glu Gly His Gly Leu Ile Tyr Phe Lys Arg Arg Phe His 
145                 150                 155                 160 


Pro Val Met Leu Glu Met Glu Ala Ala Ile 
                165                 170 


<210>  5
<211>  3983
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fragmento de sobreexpresin del complejo HpaBC

<400>  5
gagcgacctc atgctatacc tgagaaagca acctgaccta caggaaagag ttactcaaga       60

ataagaattt tcgttttaaa acctaagagt cactttaaaa tttgtataca cttatttttt      120

ttataactta tttaataata aaaatcataa atcataagaa attcgcttat ttagaagtgt      180

caacaacgta tctaccaacg gaatgcgtgc gatttaaatc gcagcttcca tttccagcat      240

caccggatgg aaacggcgtt taaagtagat aagtccgtga ccttctgcac tgaggatgat      300

gtttttaatc tccaccagat acaccagatg tgtgccaatt gcctgcacat cgcggatctc      360

accttcaaga ctggccagcg aaccttttag caccggctgc gccagcggac ctttttgcca      420

gcatgagagg ctaaaacgct cttccatcgc catgcctgtc atgcccgcga agtggcgtgc      480

catcagttcc tgctcatggt tgaggacgtt gacgcacaac ttaccgttgc cctgaaaaac      540

cgggttcatc gcactgttgg cgttaatgca caccatcagc gatggtggtg tatccgtgac      600

cgagcagacg gccgttgccg taatcccgca ttgtccggcg tcgccctcgg tggtgataat      660

atttaccgct gccgacaggc tggccattgc gtcacgaaag cgcaggcgtt gttcatctaa      720

ttgcattgtt ttacctgcac ttttgtaatt aaaacttaga ttagattgct atgctttctt      780

tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa ctgaaacttg      840

agaaattgaa gaccgtttat taacttaaat atcaatggga ggtcatcgaa agagaaaaaa      900

atcaaaaaaa aaaattttca agaaaaagaa acgtgataaa aatttttatt gcctttttcg      960

acgaagaaaa agaaacgagg cggtctcttt tttcttttcc aaacctttag tacgggtaat     1020

taacgacacc ctagaggaag aaagagggga aatttagtat gctgtgcttg ggtgttttga     1080

agtggtacgg cgatgcgcgg agtccgagaa aatctggaag agtaaaaaag gagtagaaac     1140

attttgaagc tatggtgtgt gcggccggcc tggaagtacc ttcaaagaat ggggtcttat     1200

cttgttttgc aagtaccact gagcaggata ataatagaaa tgataatata ctatagtaga     1260

gataacgtcg atgacttccc atactgtaat tgcttttagt tgtgtatttt tagtgtgcaa     1320

gtttctgtaa atcgattaat ttttttttct ttcctctttt tattaacctt aatttttatt     1380

ttagattcct gacttcaact caagacgcac agatattata acatctgcat aataggcatt     1440

tgcaagaatt actcgtgagt aaggaaagag tgaggaacta tcgcatacct gcatttaaag     1500

atgccgattt gggcgcgaat cctttatttt ggcttcaccc tcatactatt atcagggcca     1560

gaaaaaggaa gtgtttccct ccttcttgaa ttgatgttac cctcataaag cacgtggcct     1620

cttatcgaga aagaaattac cgtcgctcgt gatttgtttg caaaaagaac aaaactgaaa     1680

aaacccagac acgctcgact tcctgtcttc ctattgattg cagcttccaa tttcgtcaca     1740

caacaaggtc ctagcgacgg ctcacaggtt ttgtaacaag caatcgaagg ttctggaatg     1800

gcgggaaagg gtttagtacc acatgctatg atgcccactg tgatctccag agcaaagttc     1860

gttcgatcgt actgttactc tctctctttc aaacagaatt gtccgaatcg tgtgacaaca     1920

acagcctgtt ctcacacact cttttcttct aaccaagggg gtggtttagt ttagtagaac     1980

ctcgtgaaac ttacatttac atatatataa acttgcataa attggtcaat gcaagaaata     2040

catatttggt cttttctaat tcgtagtttt tcaagttctt agatgctttc tttttctctt     2100

ttttacagat catcaaggaa gtaattatct actttttaca acaaatataa aacaaatctg     2160

tcataaaaca atgaaaccag aagatttccg cgccagtacc caacgtccgt tcaccgggga     2220

agagtatctg aaaagcctgc aggatggtcg cgagatctat atctatggcg agcgagtgaa     2280

agacgtcact actcatccgg catttcgtaa tgcggctgcg tctgttgccc aactgtacga     2340

cgcgctacac aaaccggaga tgcaggactc tctgtgctgg aacaccgaca ccggcagcgg     2400

cggctatacc cataaattct tccgcgtggc gaaaagtgcc gacgacctgc gccacgaacg     2460

cgatgccatc gctgagtggt cacgcctgag ctatggctgg atgggccgta ccccagacta     2520

caaagctgct ttcggttgcg cactgggcgg aactccgggc ttttacggtc agttcgagca     2580

gaacgcccgt aactggtaca cccgtattca ggaaactggc ctctacttta accacgcgat     2640

tgttaaccca ccgatcgatc gtcatttgcc gaccgataaa gtaaaagacg tttacatcaa     2700

gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg ttgccaccaa     2760

ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtaa tgggcgaaaa     2820

cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtca aattaatctc     2880

ccgcgcctct tatgagatgg tcgcgggtgc taccggctca ccgtatgact acccgctctc     2940

cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtgctga tcccatggga     3000

aaacgtgctg ctctaccgcg attttgatcg ctgccgtcgc tggacgatgg aaggcggttt     3060

cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg acttcattac     3120

ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg gtgtgcaggc     3180

cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg actcgatgtg     3240

ttctgaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg cactgcaaac     3300

ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatcg aacgcaacgt     3360

taccagtggc ctgatctatc tcccttccag tgcccgtgac ctgaacaatc cgcagatcga     3420

ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc agcgcatcaa     3480

gatcctcaaa ctgatgtggg atgccattgg cagcgagttt gtttataact tatttaataa     3540

taaaaatcat aaatcataag aaattcgcgt ggtcgtcacg aactgtatga aatcaactac     3600

tccggtagcc aggatgagat tcgcctgcag tgtctgcgcc aggcacaaag ctccggcaat     3660

atggacaaga tgatggcgat ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg     3720

actgtgccgc acctgcacaa caacgacgat atcaacatgc tggataagct gctgaaataa     3780

atcgcgtgca ttcatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg     3840

tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt     3900

ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg     3960

agaaggtttt gggacgctcg aag                                             3983


<210>  6
<211>  1113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO4 K229L

<400>  6
atgagtgaat ctccaatgtt cgctgccaac ggcatgccaa aggtaaatca aggtgctgaa       60

gaagatgtca gaattttagg ttacgaccca ttagcttctc cagctctcct tcaagtgcaa      120

atcccagcca caccaacttc tttggaaact gccaagagag gtagaagaga agctatagat      180

attattaccg gtaaagacga cagagttctt gtcattgtcg gtccttgttc catccatgat      240

ctagaagccg ctcaagaata cgctttgaga ttaaagaaat tgtcagatga attaaaaggt      300

gatttatcca tcattatgag agcatacttg gagaagccaa gaacaaccgt cggctggaaa      360

ggtctaatta atgaccctga tgttaacaac actttcaaca tcaacaaggg tttgcaatcc      420

gctagacaat tgtttgtcaa cttgacaaat atcggtttgc caattggttc tgaaatgctt      480

gataccattt ctcctcaata cttggctgat ttggtctcct tcggtgccat tggtgccaga      540

accaccgaat ctcaactgca cagagaattg gcctccggtt tgtctttccc agttggtttc      600

aagaacggta ccgatggtac cttaaatgtt gctgtggatg cttgtcaagc cgctgctcat      660

tctcaccatt tcatgggtgt tactttgcat ggtgttgctg ctatcaccac tactaagggt      720

aacgaacact gcttcgttat tctaagaggt ggtaaaaagg gtaccaacta cgacgctaag      780

tccgttgcag aagctaaggc tcaattgcct gccggttcca acggtctaat gattgactac      840

tctcacggta actccaataa ggatttcaga aaccaaccaa aggtcaatga cgttgtttgt      900

gagcaaatcg ctaacggtga aaacgccatt accggtgtca tgattgaatc aaacatcaac      960

gaaggtaacc aaggcatccc agccgaaggt aaagccggct tgaaatatgg tgtttccatc     1020

actgatgctt gtataggttg ggaaactact gaagacgtct tgaggaaatt ggctgctgct     1080

gtcagacaaa gaagagaagt taacaagaaa tag                                  1113


<210>  7
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ARO4 K229L

<400>  7

Met Ser Glu Ser Pro Met Phe Ala Ala Asn Gly Met Pro Lys Val Asn 
1               5                   10                  15      


Gln Gly Ala Glu Glu Asp Val Arg Ile Leu Gly Tyr Asp Pro Leu Ala 
            20                  25                  30          


Ser Pro Ala Leu Leu Gln Val Gln Ile Pro Ala Thr Pro Thr Ser Leu 
        35                  40                  45              


Glu Thr Ala Lys Arg Gly Arg Arg Glu Ala Ile Asp Ile Ile Thr Gly 
    50                  55                  60                  


Lys Asp Asp Arg Val Leu Val Ile Val Gly Pro Cys Ser Ile His Asp 
65                  70                  75                  80  


Leu Glu Ala Ala Gln Glu Tyr Ala Leu Arg Leu Lys Lys Leu Ser Asp 
                85                  90                  95      


Glu Leu Lys Gly Asp Leu Ser Ile Ile Met Arg Ala Tyr Leu Glu Lys 
            100                 105                 110         


Pro Arg Thr Thr Val Gly Trp Lys Gly Leu Ile Asn Asp Pro Asp Val 
        115                 120                 125             


Asn Asn Thr Phe Asn Ile Asn Lys Gly Leu Gln Ser Ala Arg Gln Leu 
    130                 135                 140                 


Phe Val Asn Leu Thr Asn Ile Gly Leu Pro Ile Gly Ser Glu Met Leu 
145                 150                 155                 160 


Asp Thr Ile Ser Pro Gln Tyr Leu Ala Asp Leu Val Ser Phe Gly Ala 
                165                 170                 175     


Ile Gly Ala Arg Thr Thr Glu Ser Gln Leu His Arg Glu Leu Ala Ser 
            180                 185                 190         


Gly Leu Ser Phe Pro Val Gly Phe Lys Asn Gly Thr Asp Gly Thr Leu 
        195                 200                 205             


Asn Val Ala Val Asp Ala Cys Gln Ala Ala Ala His Ser His His Phe 
    210                 215                 220                 


Met Gly Val Thr Leu His Gly Val Ala Ala Ile Thr Thr Thr Lys Gly 
225                 230                 235                 240 


Asn Glu His Cys Phe Val Ile Leu Arg Gly Gly Lys Lys Gly Thr Asn 
                245                 250                 255     


Tyr Asp Ala Lys Ser Val Ala Glu Ala Lys Ala Gln Leu Pro Ala Gly 
            260                 265                 270         


Ser Asn Gly Leu Met Ile Asp Tyr Ser His Gly Asn Ser Asn Lys Asp 
        275                 280                 285             


Phe Arg Asn Gln Pro Lys Val Asn Asp Val Val Cys Glu Gln Ile Ala 
    290                 295                 300                 


Asn Gly Glu Asn Ala Ile Thr Gly Val Met Ile Glu Ser Asn Ile Asn 
305                 310                 315                 320 


Glu Gly Asn Gln Gly Ile Pro Ala Glu Gly Lys Ala Gly Leu Lys Tyr 
                325                 330                 335     


Gly Val Ser Ile Thr Asp Ala Cys Ile Gly Trp Glu Thr Thr Glu Asp 
            340                 345                 350         


Val Leu Arg Lys Leu Ala Ala Ala Val Arg Gln Arg Arg Glu Val Asn 
        355                 360                 365             


Lys Lys 
    370 


<210>  8
<211>  1991
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fragmento de sobreexpresin de ARO4 K229L

<400>  8
agtttatcat tatcaatact cgccatttca aagaatacgt aaataattaa tagtagtgat       60

tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc      120

ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt      180

tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa      240

aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc      300

tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat      360

ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat      420

ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga      480

aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa      540

agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact      600

tttatagtta gtcttttttt tagttttaaa acaccagaac ttagtttcga cggattctag      660

aactagtgga tccaaatgag tgaatctcca atgttcgctg ccaacggcat gccaaaggta      720

aatcaaggtg ctgaagaaga tgtcagaatt ttaggttacg acccattagc ttctccagct      780

ctccttcaag tgcaaatccc agccacacca acttctttgg aaactgccaa gagaggtaga      840

agagaagcta tagatattat taccggtaaa gacgacagag ttcttgtcat tgtcggtcct      900

tgttccatcc atgatctaga agccgctcaa gaatacgctt tgagattaaa gaaattgtca      960

gatgaattaa aaggtgattt atccatcatt atgagagcat acttggagaa gccaagaaca     1020

accgtcggct ggaaaggtct aattaatgac cctgatgtta acaacacttt caacatcaac     1080

aagggtttgc aatccgctag acaattgttt gtcaacttga caaatatcgg tttgccaatt     1140

ggttctgaaa tgcttgatac catttctcct caatacttgg ctgatttggt ctccttcggt     1200

gccattggtg ccagaaccac cgaatctcaa ctgcacagag aattggcctc cggtttgtct     1260

ttcccagttg gtttcaagaa cggtaccgat ggtaccttaa atgttgctgt ggatgcttgt     1320

caagccgctg ctcattctca ccatttcatg ggtgttactt tgcatggtgt tgctgctatc     1380

accactacta agggtaacga acactgcttc gttattctaa gaggtggtaa aaagggtacc     1440

aactacgacg ctaagtccgt tgcagaagct aaggctcaat tgcctgccgg ttccaacggt     1500

ctaatgattg actactctca cggtaactcc aataaggatt tcagaaacca accaaaggtc     1560

aatgacgttg tttgtgagca aatcgctaac ggtgaaaacg ccattaccgg tgtcatgatt     1620

gaatcaaaca tcaacgaagg taaccaaggc atcccagccg aaggtaaagc cggcttgaaa     1680

tatggtgttt ccatcactga tgcttgtata ggttgggaaa ctactgaaga cgtcttgagg     1740

aaattggctg ctgctgtcag acaaagaaga gaagttaaca agaaatagat cgcgtgcatt     1800

catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc cctatttatt     1860

tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt cttttttttc     1920

tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag aaggttttgg     1980

gacgctcgaa g                                                          1991


<210>  9
<211>  1113
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  9
atgagtgaat ctccaatgtt cgctgccaac ggcatgccaa aggtaaatca aggtgctgaa       60

gaagatgtca gaattttagg ttacgaccca ttagcttctc cagctctcct tcaagtgcaa      120

atcccagcca caccaacttc tttggaaact gccaagagag gtagaagaga agctatagat      180

attattaccg gtaaagacga cagagttctt gtcattgtcg gtccttgttc catccatgat      240

ctagaagccg ctcaagaata cgctttgaga ttaaagaaat tgtcagatga attaaaaggt      300

gatttatcca tcattatgag agcatacttg gagaagccaa gaacaaccgt cggctggaaa      360

ggtctaatta atgaccctga tgttaacaac actttcaaca tcaacaaggg tttgcaatcc      420

gctagacaat tgtttgtcaa cttgacaaat atcggtttgc caattggttc tgaaatgctt      480

gataccattt ctcctcaata cttggctgat ttggtctcct tcggtgccat tggtgccaga      540

accaccgaat ctcaactgca cagagaattg gcctccggtt tgtctttccc agttggtttc      600

aagaacggta ccgatggtac cttaaatgtt gctgtggatg cttgtcaagc cgctgctcat      660

tctcaccatt tcatgggtgt tactaagcat ggtgttgctg ctatcaccac tactaagggt      720

aacgaacact gcttcgttat tctaagaggt ggtaaaaagg gtaccaacta cgacgctaag      780

tccgttgcag aagctaaggc tcaattgcct gccggttcca acggtctaat gattgactac      840

tctcacggta actccaataa ggatttcaga aaccaaccaa aggtcaatga cgttgtttgt      900

gagcaaatcg ctaacggtga aaacgccatt accggtgtca tgattgaatc aaacatcaac      960

gaaggtaacc aaggcatccc agccgaaggt aaagccggct tgaaatatgg tgtttccatc     1020

actgatgctt gtataggttg ggaaactact gaagacgtct tgaggaaatt ggctgctgct     1080

gtcagacaaa gaagagaagt taacaagaaa tag                                  1113


<210>  10
<211>  1113
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  10
atgttcatta aaaacgatca cgccggtgac aggaaacgct tggaagactg gagaatcaaa       60

ggttatgatc cattaacccc tccagatctg cttcaacatg aatttccaat ttcagccaaa      120

ggtgaggaaa acattatcaa ggcaagagac tccgtctgtg atattttgaa tggtaaagat      180

gatcgtttag ttatcgtgat cgggccatgt tccctacatg accccaaagc cgcttacgat      240

tacgctgaca gattggctaa aatttcagaa aagttgtcaa aagacttatt gattattatg      300

agagcgtatt tagaaaaacc aaggactact gttggctgga aagggttgat taacgaccct      360

gatatgaata actcttttca aatcaataaa ggtctacgga tttcgagaga aatgttcata      420

aaactggttg aaaaattacc cattgctggt gagatgttgg ataccatttc tccgcagttt      480

ttgagtgatt gtttctcctt gggtgccatc ggcgccagaa ctactgaatc ccaactgcac      540

agagaattag catccggtct atctttccct attggattta agaacggtac tgatggtggt      600

ttgcaagtcg ccatcgacgc tatgagagcc gctgcacatg aacattactt cctttctgtc      660

acaaagccag gtgtcactgc tatcgtgggc actgaaggta acaaggatac cttcctgatc      720

ttgagaggtg gtaagaacgg tactaacttt gacaaagaaa gtgttcaaaa tactaagaaa      780

cagttagaaa aggccggttt gactgatgat tcccagaaaa gaattatgat cgattgttcc      840

cacggcaaca gtaataaaga tttcaagaac caaccaaagg ttgccaaatg tatttatgac      900

cagctgacgg agggtgagaa tagtctctgt ggtgttatga ttgagtccaa cataaatgaa      960

ggtagacaag atattcccaa agaaggtggc agagagggat tgaagtatgg ttgttctgtt     1020

acggatgctt gtattggctg ggagtccacc gaacaggtat tggagctatt ggcagaaggt     1080

gttagaaaca gaagaaaggc cttgaaaaaa tag                                  1113


<210>  11
<211>  1113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO3 K222L

<400>  11
atgttcatta aaaacgatca cgccggtgac aggaaacgct tggaagactg gagaatcaaa       60

ggttatgatc cattaacccc tccagatctg cttcaacatg aatttccaat ttcagccaaa      120

ggtgaggaaa acattatcaa ggcaagagac tccgtctgtg atattttgaa tggtaaagat      180

gatcgtttag ttatcgtgat cgggccatgt tccctacatg accccaaagc cgcttacgat      240

tacgctgaca gattggctaa aatttcagaa aagttgtcaa aagacttatt gattattatg      300

agagcgtatt tagaaaaacc aaggactact gttggctgga aagggttgat taacgaccct      360

gatatgaata actcttttca aatcaataaa ggtctacgga tttcgagaga aatgttcata      420

aaactggttg aaaaattacc cattgctggt gagatgttgg ataccatttc tccgcagttt      480

ttgagtgatt gtttctcctt gggtgccatc ggcgccagaa ctactgaatc ccaactgcac      540

agagaattag catccggtct atctttccct attggattta agaacggtac tgatggtggt      600

ttgcaagtcg ccatcgacgc tatgagagcc gctgcacatg aacattactt cctttctgtc      660

acattgccag gtgtcactgc tatcgtgggc actgaaggta acaaggatac cttcctgatc      720

ttgagaggtg gtaagaacgg tactaacttt gacaaagaaa gtgttcaaaa tactaagaaa      780

cagttagaaa aggccggttt gactgatgat tcccagaaaa gaattatgat cgattgttcc      840

cacggcaaca gtaataaaga tttcaagaac caaccaaagg ttgccaaatg tatttatgac      900

cagctgacgg agggtgagaa tagtctctgt ggtgttatga ttgagtccaa cataaatgaa      960

ggtagacaag atattcccaa agaaggtggc agagagggat tgaagtatgg ttgttctgtt     1020

acggatgctt gtattggctg ggagtccacc gaacaggtat tggagctatt ggcagaaggt     1080

gttagaaaca gaagaaaggc cttgaaaaaa tag                                  1113


<210>  12
<211>  771
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  12
atggatttca caaaaccaga aactgtttta aatctacaaa atattagaga tgaattagtt       60

agaatggagg attcgatcat cttcaaattt attgagaggt cgcatttcgc cacatgtcct      120

tcagtttatg aggcaaacca tccaggttta gaaattccga attttaaagg atctttcttg      180

gattgggctc tttcaaatct tgaaattgcg cattctcgca tcagaagatt cgaatcacct      240

gatgaaactc ccttctttcc tgacaagatt cagaaatcat tcttaccgag cattaactac      300

ccacaaattt tggcgcctta tgccccagaa gttaattaca atgataaaat aaaaaaagtt      360

tatattgaaa agattatacc attaatttcg aaaagagatg gtgatgataa gaataacttc      420

ggttctgttg ccactagaga tatagaatgt ttgcaaagct tgagtaggag aatccacttt      480

ggcaagtttg ttgctgaagc caagttccaa tcggatatcc cgctatacac aaagctgatc      540

aaaagtaaag atgtcgaggg gataatgaag aatatcacca attctgccgt tgaagaaaag      600

attctagaaa gattaactaa gaaggctgaa gtctatggtg tggaccctac caacgagtca      660

ggtgaaagaa ggattactcc agaatatttg gtaaaaattt ataaggaaat tgttatacct      720

atcactaagg aagttgaggt ggaatacttg ctaagaaggt tggaagagta a               771


<210>  13
<211>  771
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO7 G141S

<400>  13
atggatttca caaaaccaga aactgtttta aatctacaaa atattagaga tgaattagtt       60

agaatggagg attcgatcat cttcaaattt attgagaggt cgcatttcgc cacatgtcct      120

tcagtttatg aggcaaacca tccaggttta gaaattccga attttaaagg atctttcttg      180

gattgggctc tttcaaatct tgaaattgcg cattctcgca tcagaagatt cgaatcacct      240

gatgaaactc ccttctttcc tgacaagatt cagaaatcat tcttaccgag cattaactac      300

ccacaaattt tggcgcctta tgccccagaa gttaattaca atgataaaat aaaaaaagtt      360

tatattgaaa agattatacc attaatttcg aaaagagatg gtgatgataa gaataacttc      420

tcttctgttg ccactagaga tatagaatgt ttgcaaagct tgagtaggag aatccacttt      480

ggcaagtttg ttgctgaagc caagttccaa tcggatatcc cgctatacac aaagctgatc      540

aaaagtaaag atgtcgaggg gataatgaag aatatcacca attctgccgt tgaagaaaag      600

attctagaaa gattaactaa gaaggctgaa gtctatggtg tggaccctac caacgagtca      660

ggtgaaagaa ggattactcc agaatatttg gtaaaaattt ataaggaaat tgttatacct      720

atcactaagg aagttgaggt ggaatacttg ctaagaaggt tggaagagta a               771


<210>  14
<211>  1908
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  14
atggcacctg ttacaattga aaagttcgta aatcaagaag aacgacacct tgtttccaac       60

cgatcagcaa caattccgtt tggtgaatac atatttaaaa gattgttgtc catcgatacg      120

aaatcagttt tcggtgttcc tggtgacttc aacttatctc tattagaata tctctattca      180

cctagtgttg aatcagctgg cctaagatgg gtcggcacgt gtaatgaact gaacgccgct      240

tatgcggccg acggatattc ccgttactct aataagattg gctgtttaat aaccacgtat      300

ggcgttggtg aattaagcgc cttgaacggt atagccggtt cgttcgctga aaatgtcaaa      360

gttttgcaca ttgttggtgt ggccaagtcc atagattcgc gttcaagtaa ctttagtgat      420

cggaacctac atcatttggt cccacagcta catgattcaa attttaaagg gccaaatcat      480

aaagtatatc atgatatggt aaaagataga gtcgcttgct cggtagccta cttggaggat      540

attgaaactg catgtgacca agtcgataat gttatccgcg atatttacaa gtattctaaa      600

cctggttata tttttgttcc tgcagatttt gcggatatgt ctgttacatg tgataatttg      660

gttaatgttc cacgtatatc tcaacaagat tgtatagtat acccttctga aaaccaattg      720

tctgacataa tcaacaagat tactagttgg atatattcca gtaaaacacc tgcgatcctt      780

ggagacgtac tgactgatag gtatggtgtg agtaactttt tgaacaagct tatctgcaaa      840

actgggattt ggaatttttc cactgttatg ggaaaatctg taattgatga gtcaaaccca      900

acttatatgg gtcaatataa tggtaaagaa ggtttaaaac aagtctatga acattttgaa      960

ctgtgcgact tggtcttgca ttttggagtc gacatcaatg aaattaataa tgggcattat     1020

acttttactt ataaaccaaa tgctaaaatc attcaatttc atccgaatta tattcgcctt     1080

gtggacacta ggcagggcaa tgagcaaatg ttcaaaggaa tcaattttgc ccctatttta     1140

aaagaactat acaagcgcat tgacgtttct aaactttctt tgcaatatga ttcaaatgta     1200

actcaatata cgaacgaaac aatgcggtta gaagatccta ccaatggaca atcaagcatt     1260

attacacaag ttcacttaca aaagacgatg cctaaatttt tgaaccctgg tgatgttgtc     1320

gtttgtgaaa caggctcttt tcaattctct gttcgtgatt tcgcgtttcc ttcgcaatta     1380

aaatatatat cgcaaggatt tttcctttcc attggcatgg cccttcctgc cgccctaggt     1440

gttggaattg ccatgcaaga ccactcaaac gctcacatca atggtggcaa cgtaaaagag     1500

gactataagc caagattaat tttgtttgaa ggtgacggtg cagcacagat gacaatccaa     1560

gaactgagca ccattctgaa gtgcaatatt ccactagaag ttatcatttg gaacaataac     1620

ggctacacta ttgaaagagc catcatgggc cctaccaggt cgtataacga cgttatgtct     1680

tggaaatgga ccaaactatt tgaagcattc ggagacttcg acggaaagta tactaatagc     1740

actctcattc aatgtccctc taaattagca ctgaaattgg aggagcttaa gaattcaaac     1800

aaaagaagcg ggatagaact tttagaagtc aaattaggcg aattggattt ccccgaacag     1860

ctaaagtgca tggttgaagc agcggcactt aaaagaaata aaaaatag                  1908


<210>  15
<211>  984
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promotor PGK1

<400>  15
ggaagtacct tcaaagaatg gggtcttatc ttgttttgca agtaccactg agcaggataa       60

taatagaaat gataatatac tatagtagag ataacgtcga tgacttccca tactgtaatt      120

gcttttagtt gtgtattttt agtgtgcaag tttctgtaaa tcgattaatt tttttttctt      180

tcctcttttt attaacctta atttttattt tagattcctg acttcaactc aagacgcaca      240

gatattataa catctgcata ataggcattt gcaagaatta ctcgtgagta aggaaagagt      300

gaggaactat cgcatacctg catttaaaga tgccgatttg ggcgcgaatc ctttattttg      360

gcttcaccct catactatta tcagggccag aaaaaggaag tgtttccctc cttcttgaat      420

tgatgttacc ctcataaagc acgtggcctc ttatcgagaa agaaattacc gtcgctcgtg      480

atttgtttgc aaaaagaaca aaactgaaaa aacccagaca cgctcgactt cctgtcttcc      540

tattgattgc agcttccaat ttcgtcacac aacaaggtcc tagcgacggc tcacaggttt      600

tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg tttagtacca catgctatga      660

tgcccactgt gatctccaga gcaaagttcg ttcgatcgta ctgttactct ctctctttca      720

aacagaattg tccgaatcgt gtgacaacaa cagcctgttc tcacacactc ttttcttcta      780

accaaggggg tggtttagtt tagtagaacc tcgtgaaact tacatttaca tatatataaa      840

cttgcataaa ttggtcaatg caagaaatac atatttggtc ttttctaatt cgtagttttt      900

caagttctta gatgctttct ttttctcttt tttacagatc atcaaggaag taattatcta      960

ctttttacaa caaatataaa acaa                                             984


<210>  16
<211>  420
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promotor TEF1

<400>  16
cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac       60

tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat ttcccctctt      120

tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa aaaagagacc      180

gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg tttctttttc      240

ttgaaaattt ttttttttga tttttttctc tttcgatgac ctcccattga tatttaagtt      300

aataaacggt cttcaatttc tcaagtttca gtttcatttt tcttgttcta ttacaacttt      360

ttttacttct tgctcattag aaagaaagca tagcaatcta atctaagttt taattacaaa      420


<210>  17
<211>  1414
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biobrick promotor dual PGK1p-TEF1p

<400>  17
tttgtaatta aaacttagat tagattgcta tgctttcttt ctaatgagca agaagtaaaa       60

aaagttgtaa tagaacaaga aaaatgaaac tgaaacttga gaaattgaag accgtttatt      120

aacttaaata tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa aaattttcaa      180

gaaaaagaaa cgtgataaaa atttttattg cctttttcga cgaagaaaaa gaaacgaggc      240

ggtctctttt ttcttttcca aacctttagt acgggtaatt aacgacaccc tagaggaaga      300

aagaggggaa atttagtatg ctgtgcttgg gtgttttgaa gtggtacggc gatgcgcgga      360

gtccgagaaa atctggaaga gtaaaaaagg agtagaaaca ttttgaagct atggtgtgtg      420

cggccggcct ggaagtacct tcaaagaatg gggtcttatc ttgttttgca agtaccactg      480

agcaggataa taatagaaat gataatatac tatagtagag ataacgtcga tgacttccca      540

tactgtaatt gcttttagtt gtgtattttt agtgtgcaag tttctgtaaa tcgattaatt      600

tttttttctt tcctcttttt attaacctta atttttattt tagattcctg acttcaactc      660

aagacgcaca gatattataa catctgcata ataggcattt gcaagaatta ctcgtgagta      720

aggaaagagt gaggaactat cgcatacctg catttaaaga tgccgatttg ggcgcgaatc      780

ctttattttg gcttcaccct catactatta tcagggccag aaaaaggaag tgtttccctc      840

cttcttgaat tgatgttacc ctcataaagc acgtggcctc ttatcgagaa agaaattacc      900

gtcgctcgtg atttgtttgc aaaaagaaca aaactgaaaa aacccagaca cgctcgactt      960

cctgtcttcc tattgattgc agcttccaat ttcgtcacac aacaaggtcc tagcgacggc     1020

tcacaggttt tgtaacaagc aatcgaaggt tctggaatgg cgggaaaggg tttagtacca     1080

catgctatga tgcccactgt gatctccaga gcaaagttcg ttcgatcgta ctgttactct     1140

ctctctttca aacagaattg tccgaatcgt gtgacaacaa cagcctgttc tcacacactc     1200

ttttcttcta accaaggggg tggtttagtt tagtagaacc tcgtgaaact tacatttaca     1260

tatatataaa cttgcataaa ttggtcaatg caagaaatac atatttggtc ttttctaatt     1320

cgtagttttt caagttctta gatgctttct ttttctcttt tttacagatc atcaaggaag     1380

taattatcta ctttttacaa caaatataaa acaa                                 1414


<210>  18
<211>  1788
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  biobrick GPDp ARO4 K229L

<400>  18
agtttatcat tatcaatact cgccatttca aagaatacgt aaataattaa tagtagtgat       60

tttcctaact ttatttagtc aaaaaattag ccttttaatt ctgctgtaac ccgtacatgc      120

ccaaaatagg gggcgggtta cacagaatat ataacatcgt aggtgtctgg gtgaacagtt      180

tattcctggc atccactaaa tataatggag cccgcttttt aagctggcat ccagaaaaaa      240

aaagaatccc agcaccaaaa tattgttttc ttcaccaacc atcagttcat aggtccattc      300

tcttagcgca actacagaga acaggggcac aaacaggcaa aaaacgggca caacctcaat      360

ggagtgatgc aacctgcctg gagtaaatga tgacacaagg caattgaccc acgcatgtat      420

ctatctcatt ttcttacacc ttctattacc ttctgctctc tctgatttgg aaaaagctga      480

aaaaaaaggt tgaaaccagt tccctgaaat tattccccta cttgactaat aagtatataa      540

agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt aaattctact      600

tttatagtta gtcttttttt tagttttaaa acaccagaac ttagtttcga cggattctag      660

aactagtgga tccaaatgag tgaatctcca atgttcgctg ccaacggcat gccaaaggta      720

aatcaaggtg ctgaagaaga tgtcagaatt ttaggttacg acccattagc ttctccagct      780

ctccttcaag tgcaaatccc agccacacca acttctttgg aaactgccaa gagaggtaga      840

agagaagcta tagatattat taccggtaaa gacgacagag ttcttgtcat tgtcggtcct      900

tgttccatcc atgatctaga agccgctcaa gaatacgctt tgagattaaa gaaattgtca      960

gatgaattaa aaggtgattt atccatcatt atgagagcat acttggagaa gccaagaaca     1020

accgtcggct ggaaaggtct aattaatgac cctgatgtta acaacacttt caacatcaac     1080

aagggtttgc aatccgctag acaattgttt gtcaacttga caaatatcgg tttgccaatt     1140

ggttctgaaa tgcttgatac catttctcct caatacttgg ctgatttggt ctccttcggt     1200

gccattggtg ccagaaccac cgaatctcaa ctgcacagag aattggcctc cggtttgtct     1260

ttcccagttg gtttcaagaa cggtaccgat ggtaccttaa atgttgctgt ggatgcttgt     1320

caagccgctg ctcattctca ccatttcatg ggtgttactt tgcatggtgt tgctgctatc     1380

accactacta agggtaacga acactgcttc gttattctaa gaggtggtaa aaagggtacc     1440

aactacgacg ctaagtccgt tgcagaagct aaggctcaat tgcctgccgg ttccaacggt     1500

ctaatgattg actactctca cggtaactcc aataaggatt tcagaaacca accaaaggtc     1560

aatgacgttg tttgtgagca aatcgctaac ggtgaaaacg ccattaccgg tgtcatgatt     1620

gaatcaaaca tcaacgaagg taaccaaggc atcccagccg aaggtaaagc cggcttgaaa     1680

tatggtgttt ccatcactga tgcttgtata ggttgggaaa ctactgaaga cgtcttgagg     1740

aaattggctg ctgctgtcag acaaagaaga gaagttaaca agaaatag                  1788


<210>  19
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ARO3 K222L

<400>  19

Met Phe Ile Lys Asn Asp His Ala Gly Asp Arg Lys Arg Leu Glu Asp 
1               5                   10                  15      


Trp Arg Ile Lys Gly Tyr Asp Pro Leu Thr Pro Pro Asp Leu Leu Gln 
            20                  25                  30          


His Glu Phe Pro Ile Ser Ala Lys Gly Glu Glu Asn Ile Ile Lys Ala 
        35                  40                  45              


Arg Asp Ser Val Cys Asp Ile Leu Asn Gly Lys Asp Asp Arg Leu Val 
    50                  55                  60                  


Ile Val Ile Gly Pro Cys Ser Leu His Asp Pro Lys Ala Ala Tyr Asp 
65                  70                  75                  80  


Tyr Ala Asp Arg Leu Ala Lys Ile Ser Glu Lys Leu Ser Lys Asp Leu 
                85                  90                  95      


Leu Ile Ile Met Arg Ala Tyr Leu Glu Lys Pro Arg Thr Thr Val Gly 
            100                 105                 110         


Trp Lys Gly Leu Ile Asn Asp Pro Asp Met Asn Asn Ser Phe Gln Ile 
        115                 120                 125             


Asn Lys Gly Leu Arg Ile Ser Arg Glu Met Phe Ile Lys Leu Val Glu 
    130                 135                 140                 


Lys Leu Pro Ile Ala Gly Glu Met Leu Asp Thr Ile Ser Pro Gln Phe 
145                 150                 155                 160 


Leu Ser Asp Cys Phe Ser Leu Gly Ala Ile Gly Ala Arg Thr Thr Glu 
                165                 170                 175     


Ser Gln Leu His Arg Glu Leu Ala Ser Gly Leu Ser Phe Pro Ile Gly 
            180                 185                 190         


Phe Lys Asn Gly Thr Asp Gly Gly Leu Gln Val Ala Ile Asp Ala Met 
        195                 200                 205             


Arg Ala Ala Ala His Glu His Tyr Phe Leu Ser Val Thr Leu Pro Gly 
    210                 215                 220                 


Val Thr Ala Ile Val Gly Thr Glu Gly Asn Lys Asp Thr Phe Leu Ile 
225                 230                 235                 240 


Leu Arg Gly Gly Lys Asn Gly Thr Asn Phe Asp Lys Glu Ser Val Gln 
                245                 250                 255     


Asn Thr Lys Lys Gln Leu Glu Lys Ala Gly Leu Thr Asp Asp Ser Gln 
            260                 265                 270         


Lys Arg Ile Met Ile Asp Cys Ser His Gly Asn Ser Asn Lys Asp Phe 
        275                 280                 285             


Lys Asn Gln Pro Lys Val Ala Lys Cys Ile Tyr Asp Gln Leu Thr Glu 
    290                 295                 300                 


Gly Glu Asn Ser Leu Cys Gly Val Met Ile Glu Ser Asn Ile Asn Glu 
305                 310                 315                 320 


Gly Arg Gln Asp Ile Pro Lys Glu Gly Gly Arg Glu Gly Leu Lys Tyr 
                325                 330                 335     


Gly Cys Ser Val Thr Asp Ala Cys Ile Gly Trp Glu Ser Thr Glu Gln 
            340                 345                 350         


Val Leu Glu Leu Leu Ala Glu Gly Val Arg Asn Arg Arg Lys Ala Leu 
        355                 360                 365             


Lys Lys 
    370 


<210>  20
<211>  256
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ARO7 G141S

<400>  20

Met Asp Phe Thr Lys Pro Glu Thr Val Leu Asn Leu Gln Asn Ile Arg 
1               5                   10                  15      


Asp Glu Leu Val Arg Met Glu Asp Ser Ile Ile Phe Lys Phe Ile Glu 
            20                  25                  30          


Arg Ser His Phe Ala Thr Cys Pro Ser Val Tyr Glu Ala Asn His Pro 
        35                  40                  45              


Gly Leu Glu Ile Pro Asn Phe Lys Gly Ser Phe Leu Asp Trp Ala Leu 
    50                  55                  60                  


Ser Asn Leu Glu Ile Ala His Ser Arg Ile Arg Arg Phe Glu Ser Pro 
65                  70                  75                  80  


Asp Glu Thr Pro Phe Phe Pro Asp Lys Ile Gln Lys Ser Phe Leu Pro 
                85                  90                  95      


Ser Ile Asn Tyr Pro Gln Ile Leu Ala Pro Tyr Ala Pro Glu Val Asn 
            100                 105                 110         


Tyr Asn Asp Lys Ile Lys Lys Val Tyr Ile Glu Lys Ile Ile Pro Leu 
        115                 120                 125             


Ile Ser Lys Arg Asp Gly Asp Asp Lys Asn Asn Phe Ser Ser Val Ala 
    130                 135                 140                 


Thr Arg Asp Ile Glu Cys Leu Gln Ser Leu Ser Arg Arg Ile His Phe 
145                 150                 155                 160 


Gly Lys Phe Val Ala Glu Ala Lys Phe Gln Ser Asp Ile Pro Leu Tyr 
                165                 170                 175     


Thr Lys Leu Ile Lys Ser Lys Asp Val Glu Gly Ile Met Lys Asn Ile 
            180                 185                 190         


Thr Asn Ser Ala Val Glu Glu Lys Ile Leu Glu Arg Leu Thr Lys Lys 
        195                 200                 205             


Ala Glu Val Tyr Gly Val Asp Pro Thr Asn Glu Ser Gly Glu Arg Arg 
    210                 215                 220                 


Ile Thr Pro Glu Tyr Leu Val Lys Ile Tyr Lys Glu Ile Val Ile Pro 
225                 230                 235                 240 


Ile Thr Lys Glu Val Glu Val Glu Tyr Leu Leu Arg Arg Leu Glu Glu 
                245                 250                 255     


<210>  21
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO3 F BamHI

<400>  21
gtcgtgggat ccttatgttc attaaaaacg atcacgccgg tg                          42


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO3 K222L R

<400>  22
gcagtgacac ctggcaatgt gacagaaagg                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO3 K222L F

<400>  23
cctttctgtc acattgccag gtgtcactgc                                        30


<210>  24
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO3 R XhoI

<400>  24
ccgctcgagc tattttttca aggcctttct tctgtttcta acacc                       45


<210>  25
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO4 F BamHI

<400>  25
gtcgtgggat ccaaatgagt gaatctccaa tgttcgctgc caac                        44


<210>  26
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO4 K229L R

<400>  26
gcagcaacac catgcaaagt aacacccatg                                        30


<210>  27
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO4 R XhoI

<400>  27
ccgctcgagc tatttcttgt taacttctct tctttgtctg acagc                       45


<210>  28
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO4 K229L F

<400>  28
catgggtgtt actttgcatg gtgttgctgc                                        30


<210>  29
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO7 F BamHI

<400>  29
gtcgtgggat ccatatggat ttcacaaaac cagaaactgt tt                          42


<210>  30
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO7 G141S R

<400>  30
ctagtggcaa cagaagagaa gttattctta                                        30


<210>  31
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO7 G141S F

<400>  31
taagaataac ttctcttctg ttgccactag                                        30


<210>  32
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ARO7 R XhoI

<400>  32
ccgctcgagt tactcttcca accttcttag caagtattcc                             40


<210>  33
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPDPro-F

<400>  33
cggtaggtat tgattgtaat tctg                                              24


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYC1-R

<400>  34
gcgtgaatgt aagcgtgac                                                    19


<210>  35
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  URA3 STOP F

<400>  35
tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt taaaacttgc gctcaattcc       60


<210>  36
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  URA3 STOP R

<400>  36
catgtgtttt tagtaaacaa attttgggac ctaatgcttc ggaattgagc gcaagtttta       60


<210>  37
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LEU2 STOP F

<400>  37
tcgttccaat gtcaagttcg atttcgaaaa tcatttaatt taaaacttgc gctcaattcc       60


<210>  38
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LEU2 STOP R

<400>  38
aaaacggcat cagccttctt ggaggcttcc agcgcctcat ggaattgagc gcaagtttta       60


<210>  39
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hpaB BamHI F

<400>  39
aggtcgtggg atccccatga aaccagaaga tttccg                                 36


<210>  40
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hpaB XhoI R

<400>  40
ccgctcgagt tatttcagca gcttatccag c                                      31


<210>  41
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hpaC BamHI F

<400>  41
aggtcgtggg atccccatgc aattagatga acaacgc                                37


<210>  42
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  hpaC XhoI R

<400>  42
ccgctcgagt taaatcgcag cttccatttc c                                      31


<210>  43
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GV1R (HpaC)

<400>  43
cgtgcgautt aaatcgcagc ttccatttcc                                        30


<210>  44
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GP1F (HpaC)

<400>  44
agtgcaggua aaacaatgca attagatgaa caacgc                                 36


<210>  45
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PG1R(TEF1p)

<400>  45
acctgcacut ttgtaattaa aacttagatt agattgctat                             40


<210>  46
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PG2R (PGK1p)

<400>  46
atgacagaut tgttttatat ttgttgtaaa aagtagataa tt                          42


<210>  47
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GP2F (HpaB)

<400>  47
atctgtcaua aaacaatgaa accagaagat ttccg                                  35


<210>  48
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GV2R(HpaB)

<400>  48
cacgcgautt atttcagcag cttatccagc                                        30


<210>  49
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PV2F (GPDp)

<400>  49
cgtgcgauag tttatcatta tcaatactcg cc                                     32


<210>  50
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GV2R (ARO4)

<400>  50
cacgcgauct atttcttgtt aacttctctt ctttgtct                               38


<210>  51
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ADH1_test_fw

<400>  51
gaaattcgct tatttagaag tgtc                                              24


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYC1_test_rv

<400>  52
ctccttcctt ttcggttaga g                                                 21


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X-3903 Up

<400>  53
tgacgaatcg ttaggcacag                                                   20


<210>  54
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2221

<400>  54
gttgacactt ctaaataagc gaatttc                                           27


<210>  55
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2220

<400>  55
cctgcaggac tagtgctgag                                                   20


<210>  56
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  X-3904 Down

<400>  56
ccgtgcaata ccaaaatcg                                                    19


<210>  57
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Diana de pWS173 URA3 target

<400>  57
attggatgtt cgtaccacca agg                                               23


<210>  58
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Diana de pWS174 URA3 target

<400>  58
ggtgctgcta tcgatgctac agg                                               23


