                         SEQUENCE LISTING

<110>  BlueSky Immunotherapies GmbH
 
<120>  NOVEL REPLICATION DEFICIENT INFLUENZA A VIRUS INDUCING HIGH 
       LEVELS OF TYPE I INTERFERON

<130>  MT008P

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of full length NS1 protein

<400>  1

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              


Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  


Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  


Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      


Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala 
            100                 105                 110         


Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 
        115                 120                 125             


Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 
    130                 135                 140                 


Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile 
145                 150                 155                 160 


Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn 
                165                 170                 175     


Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 
            180                 185                 190         


Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 
        195                 200                 205             


Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly 
    210                 215                 220                 


Thr Ile Arg Ser Glu Val 
225                 230 


<210>  2
<211>  890
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide sequence of full length NS gene

<400>  2
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagc accctcggtc      180

tggacatcga gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagaag      240

aatccgatga ggcacttaaa atgaccatgg cctctgtacc tgcgtcgcgt tacctaactg      300

acatgactct tgaggaaatg tcaagggact ggtccatgct catacccaag cagaaagtgg      360

caggccctct ttgtatcaga atggaccagg cgatcatgga taagaacatc atactgaaag      420

cgaacttcag tgtgattttt gaccggctgg agactctaat attgctaagg gctttcaccg      480

aagagggagc aattgttggc gaaatttcac cattgccttc tcttccagga catactgctg      540

aggatgtcaa aaatgcagtt ggagtcctca tcgggggact tgaatggaat gataacacag      600

ttcgagtctc tgaaactcta cagagattcg cttggagaag cagtaatgag aatgggagac      660

ctccactcac tccaaaacag aaacgagaaa tggcgggaac aattaggtca gaagtttgaa      720

gaaataagat ggttgattga agaagtgaga cacaaactga agataacaga gaatagtttt      780

gagcaaataa catttatgca agccttacat ctattgcttg aagtggagca agagataaga      840

actttctcgt ttcagcttat ttaataataa aaaacaccct tgtttctact                 890


<210>  3
<211>  171
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence DelNS15-73

<400>  3

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Asp Glu 
1               5                   10                  15      


Ala Leu Lys Met Thr Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr 
            20                  25                  30          


Asp Met Thr Leu Glu Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro 
        35                  40                  45              


Lys Gln Lys Val Ala Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile 
    50                  55                  60                  


Met Asp Lys Asn Ile Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp 
65                  70                  75                  80  


Arg Leu Glu Thr Leu Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala 
                85                  90                  95      


Ile Val Gly Glu Ile Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala 
            100                 105                 110         


Glu Asp Val Lys Asn Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp 
        115                 120                 125             


Asn Asp Asn Thr Val Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp 
    130                 135                 140                 


Arg Ser Ser Asn Glu Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys 
145                 150                 155                 160 


Arg Glu Met Ala Gly Thr Ile Arg Ser Glu Val 
                165                 170     


<210>  4
<211>  713
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence DelNS15-73

<400>  4
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttga tgaggcactt aaaatgacca tggcctctgt acctgcgtcg cgttacctaa      120

ctgacatgac tcttgaggaa atgtcaaggg actggtccat gctcataccc aagcagaaag      180

tggcaggccc tctttgtatc agaatggacc aggcgatcat ggataagaac atcatactga      240

aagcgaactt cagtgtgatt tttgaccggc tggagactct aatattgcta agggctttca      300

ccgaagaggg agcaattgtt ggcgaaattt caccattgcc ttctcttcca ggacatactg      360

ctgaggatgt caaaaatgca gttggagtcc tcatcggggg acttgaatgg aatgataaca      420

cagttcgagt ctctgaaact ctacagagat tcgcttggag aagcagtaat gagaatggga      480

gacctccact cactccaaaa cagaaacgag aaatggcggg aacaattagg tcagaagttt      540

gaagaaataa gatggttgat tgaagaagtg agacacaaac tgaagataac agagaatagt      600

tttgagcaaa taacatttat gcaagcctta catctattgc ttgaagtgga gcaagagata      660

agaactttct cgtttcagct tatttaataa taaaaaacac ccttgtttct act             713


<210>  5
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DelNS35-50

<400>  5

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
        35                  40                  45              


Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
    50                  55                  60                  


Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
65                  70                  75                  80  


Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala 
                85                  90                  95      


Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 
            100                 105                 110         


Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 
        115                 120                 125             


Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile 
    130                 135                 140                 


Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn 
145                 150                 155                 160 


Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 
                165                 170                 175     


Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 
            180                 185                 190         


Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly 
        195                 200                 205             


Thr Ile Arg Ser Glu Val 
    210                 


<210>  6
<211>  842
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence of DelNS35-50

<400>  6
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatgg tctggacatc gagacagcca cacgtgctgg aaagcagata gtggagcgga      180

ttctgaaaga agaatccgat gaggcactta aaatgaccat ggcctctgta cctgcgtcgc      240

gttacctaac tgacatgact cttgaggaaa tgtcaaggga ctggtccatg ctcataccca      300

agcagaaagt ggcaggccct ctttgtatca gaatggacca ggcgatcatg gataagaaca      360

tcatactgaa agcgaacttc agtgtgattt ttgaccggct ggagactcta atattgctaa      420

gggctttcac cgaagaggga gcaattgttg gcgaaatttc accattgcct tctcttccag      480

gacatactgc tgaggatgtc aaaaatgcag ttggagtcct catcggggga cttgaatgga      540

atgataacac agttcgagtc tctgaaactc tacagagatt cgcttggaga agcagtaatg      600

agaatgggag acctccactc actccaaaac agaaacgaga aatggcggga acaattaggt      660

cagaagtttg aagaaataag atggttgatt gaagaagtga gacacaaact gaagataaca      720

gagaatagtt ttgagcaaat aacatttatg caagccttac atctattgct tgaagtggag      780

caagagataa gaactttctc gtttcagctt atttaataat aaaaaacacc cttgtttcta      840

ct                                                                     842


<210>  7
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DelNS35-70

<400>  7

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Glu Glu Ser Asp Glu Ala Leu Lys Met Thr Met Ala Ser Val 
        35                  40                  45              


Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu Glu Met Ser Arg 
    50                  55                  60                  


Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala Gly Pro Leu Cys 
65                  70                  75                  80  


Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile Ile Leu Lys Ala 
                85                  90                  95      


Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu Ile Leu Leu Arg 
            100                 105                 110         


Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile Ser Pro Leu Pro 
        115                 120                 125             


Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn Ala Val Gly Val 
    130                 135                 140                 


Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val Arg Val Ser Glu 
145                 150                 155                 160 


Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu Asn Gly Arg Pro 
                165                 170                 175     


Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly Thr Ile Arg Ser 
            180                 185                 190         


Glu Val 
        


<210>  8
<211>  782
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence of DelNS35-70

<400>  8
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatga agaatccgat gaggcactta aaatgaccat ggcctctgta cctgcgtcgc      180

gttacctaac tgacatgact cttgaggaaa tgtcaaggga ctggtccatg ctcataccca      240

agcagaaagt ggcaggccct ctttgtatca gaatggacca ggcgatcatg gataagaaca      300

tcatactgaa agcgaacttc agtgtgattt ttgaccggct ggagactcta atattgctaa      360

gggctttcac cgaagaggga gcaattgttg gcgaaatttc accattgcct tctcttccag      420

gacatactgc tgaggatgtc aaaaatgcag ttggagtcct catcggggga cttgaatgga      480

atgataacac agttcgagtc tctgaaactc tacagagatt cgcttggaga agcagtaatg      540

agaatgggag acctccactc actccaaaac agaaacgaga aatggcggga acaattaggt      600

cagaagtttg aagaaataag atggttgatt gaagaagtga gacacaaact gaagataaca      660

gagaatagtt ttgagcaaat aacatttatg caagccttac atctattgct tgaagtggag      720

caagagataa gaactttctc gtttcagctt atttaataat aaaaaacacc cttgtttcta      780

ct                                                                     782


<210>  9
<211>  209
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DelNS40-60

<400>  9

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Arg Leu Arg Arg Asp Gly Lys Gln Ile Val Glu Arg Ile Leu 
        35                  40                  45              


Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr Met Ala Ser Val Pro 
    50                  55                  60                  


Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu Glu Met Ser Arg Asp 
65                  70                  75                  80  


Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala Gly Pro Leu Cys Ile 
                85                  90                  95      


Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile Ile Leu Lys Ala Asn 
            100                 105                 110         


Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu Ile Leu Leu Arg Ala 
        115                 120                 125             


Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile Ser Pro Leu Pro Ser 
    130                 135                 140                 


Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn Ala Val Gly Val Leu 
145                 150                 155                 160 


Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val Arg Val Ser Glu Thr 
                165                 170                 175     


Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu Asn Gly Arg Pro Pro 
            180                 185                 190         


Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly Thr Ile Arg Ser Glu 
        195                 200                 205             


Val 
    


<210>  10
<211>  827
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence of DelNS40-60

<400>  10
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatcg gcttcgccga gatggaaagc agatagtgga gcggattctg aaagaagaat      180

ccgatgaggc acttaaaatg accatggcct ctgtacctgc gtcgcgttac ctaactgaca      240

tgactcttga ggaaatgtca agggactggt ccatgctcat acccaagcag aaagtggcag      300

gccctctttg tatcagaatg gaccaggcga tcatggataa gaacatcata ctgaaagcga      360

acttcagtgt gatttttgac cggctggaga ctctaatatt gctaagggct ttcaccgaag      420

agggagcaat tgttggcgaa atttcaccat tgccttctct tccaggacat actgctgagg      480

atgtcaaaaa tgcagttgga gtcctcatcg ggggacttga atggaatgat aacacagttc      540

gagtctctga aactctacag agattcgctt ggagaagcag taatgagaat gggagacctc      600

cactcactcc aaaacagaaa cgagaaatgg cgggaacaat taggtcagaa gtttgaagaa      660

ataagatggt tgattgaaga agtgagacac aaactgaaga taacagagaa tagttttgag      720

caaataacat ttatgcaagc cttacatcta ttgcttgaag tggagcaaga gataagaact      780

ttctcgtttc agcttattta ataataaaaa acacccttgt ttctact                    827


<210>  11
<211>  189
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of DelNS40-80

<400>  11

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Arg Leu Arg Arg Asp Met Ala Ser Val Pro Ala Ser Arg Tyr 
        35                  40                  45              


Leu Thr Asp Met Thr Leu Glu Glu Met Ser Arg Asp Trp Ser Met Leu 
    50                  55                  60                  


Ile Pro Lys Gln Lys Val Ala Gly Pro Leu Cys Ile Arg Met Asp Gln 
65                  70                  75                  80  


Ala Ile Met Asp Lys Asn Ile Ile Leu Lys Ala Asn Phe Ser Val Ile 
                85                  90                  95      


Phe Asp Arg Leu Glu Thr Leu Ile Leu Leu Arg Ala Phe Thr Glu Glu 
            100                 105                 110         


Gly Ala Ile Val Gly Glu Ile Ser Pro Leu Pro Ser Leu Pro Gly His 
        115                 120                 125             


Thr Ala Glu Asp Val Lys Asn Ala Val Gly Val Leu Ile Gly Gly Leu 
    130                 135                 140                 


Glu Trp Asn Asp Asn Thr Val Arg Val Ser Glu Thr Leu Gln Arg Phe 
145                 150                 155                 160 


Ala Trp Arg Ser Ser Asn Glu Asn Gly Arg Pro Pro Leu Thr Pro Lys 
                165                 170                 175     


Gln Lys Arg Glu Met Ala Gly Thr Ile Arg Ser Glu Val 
            180                 185                 


<210>  12
<211>  767
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nt sequence of DelNS40-80

<400>  12
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatcg gcttcgccga gatatggcct ctgtacctgc gtcgcgttac ctaactgaca      180

tgactcttga ggaaatgtca agggactggt ccatgctcat acccaagcag aaagtggcag      240

gccctctttg tatcagaatg gaccaggcga tcatggataa gaacatcata ctgaaagcga      300

acttcagtgt gatttttgac cggctggaga ctctaatatt gctaagggct ttcaccgaag      360

agggagcaat tgttggcgaa atttcaccat tgccttctct tccaggacat actgctgagg      420

atgtcaaaaa tgcagttgga gtcctcatcg ggggacttga atggaatgat aacacagttc      480

gagtctctga aactctacag agattcgctt ggagaagcag taatgagaat gggagacctc      540

cactcactcc aaaacagaaa cgagaaatgg cgggaacaat taggtcagaa gtttgaagaa      600

ataagatggt tgattgaaga agtgagacac aaactgaaga taacagagaa tagttttgag      660

caaataacat ttatgcaagc cttacatcta ttgcttgaag tggagcaaga gataagaact      720

ttctcgtttc agcttattta ataataaaaa acacccttgt ttctact                    767


