                         SEQUENCE LISTING

<110>  OSPEDALE SAN RAFFAELE S.R.L.
 
<120>  AGENTS AND METHODS FOR INCREASING LIVER IMMUNE RESPONSE

<130>  P120879PCT

<140>  PCT/EP2021/083225
<141>  2021-11-26

<150>  GB2018657.3
<151>  2020-11-26

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  153
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-2

<400>  1

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu 
            20                  25                  30          


Gln Leu Glu His Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile 
        35                  40                  45              


Asn Asn Tyr Lys Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe 
    50                  55                  60                  


Tyr Met Pro Lys Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu 
65                  70                  75                  80  


Glu Glu Leu Lys Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys 
                85                  90                  95      


Asn Phe His Leu Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile 
            100                 105                 110         


Val Leu Glu Leu Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala 
        115                 120                 125             


Asp Glu Thr Ala Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe 
    130                 135                 140                 


Cys Gln Ser Ile Ile Ser Thr Leu Thr 
145                 150             


<210>  2
<211>  462
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-2

<400>  2
atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacaaacagt       60

gcacctactt caagttctac aaagaaaaca cagctacaac tggagcattt actgctggat      120

ttacagatga ttttgaatgg aattaataat tacaagaatc ccaaactcac caggatgctc      180

acatttaagt tttacatgcc caagaaggcc acagaactga aacatcttca gtgtctagaa      240

gaagaactca aacctctgga ggaagtgcta aatttagctc aaagcaaaaa ctttcactta      300

agacccaggg acttaatcag caatatcaac gtaatagttc tggaactaaa gggatctgaa      360

acaacattca tgtgtgaata tgctgatgag acagcaacca ttgtagaatt tctgaacaga      420

tggattacct tttgtcaaag catcatctca acactgactt ga                         462


<210>  3
<211>  177
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-7

<400>  3

Met Phe His Val Ser Phe Arg Tyr Ile Phe Gly Leu Pro Pro Leu Ile 
1               5                   10                  15      


Leu Val Leu Leu Pro Val Ala Ser Ser Asp Cys Asp Ile Glu Gly Lys 
            20                  25                  30          


Asp Gly Lys Gln Tyr Glu Ser Val Leu Met Val Ser Ile Asp Gln Leu 
        35                  40                  45              


Leu Asp Ser Met Lys Glu Ile Gly Ser Asn Cys Leu Asn Asn Glu Phe 
    50                  55                  60                  


Asn Phe Phe Lys Arg His Ile Cys Asp Ala Asn Lys Glu Gly Met Phe 
65                  70                  75                  80  


Leu Phe Arg Ala Ala Arg Lys Leu Arg Gln Phe Leu Lys Met Asn Ser 
                85                  90                  95      


Thr Gly Asp Phe Asp Leu His Leu Leu Lys Val Ser Glu Gly Thr Thr 
            100                 105                 110         


Ile Leu Leu Asn Cys Thr Gly Gln Val Lys Gly Arg Lys Pro Ala Ala 
        115                 120                 125             


Leu Gly Glu Ala Gln Pro Thr Lys Ser Leu Glu Glu Asn Lys Ser Leu 
    130                 135                 140                 


Lys Glu Gln Lys Lys Leu Asn Asp Leu Cys Phe Leu Lys Arg Leu Leu 
145                 150                 155                 160 


Gln Glu Ile Lys Thr Cys Trp Asn Lys Ile Leu Met Gly Thr Lys Glu 
                165                 170                 175     


His 
    


<210>  4
<211>  534
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-7

<400>  4
atgttccatg tttcttttag gtatatcttt ggacttcctc ccctgatcct tgttctgttg       60

ccagtagcat catctgattg tgatattgaa ggtaaagatg gcaaacaata tgagagtgtt      120

ctaatggtca gcatcgatca attattggac agcatgaaag aaattggtag caattgcctg      180

aataatgaat ttaacttttt taaaagacat atctgtgatg ctaataagga aggtatgttt      240

ttattccgtg ctgctcgcaa gttgaggcaa tttcttaaaa tgaatagcac tggtgatttt      300

gatctccact tattaaaagt ttcagaaggc acaacaatac tgttgaactg cactggccag      360

gttaaaggaa gaaaaccagc tgccctgggt gaagcccaac caacaaagag tttggaagaa      420

aataaatctt taaaggaaca gaaaaaactg aatgacttgt gtttcctaaa gagactatta      480

caagagataa aaacttgttg gaataaaatt ttgatgggca ctaaagaaca ctga            534


<210>  5
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IL-15

<400>  5

Met Arg Ile Ser Lys Pro His Leu Arg Ser Ile Ser Ile Gln Cys Tyr 
1               5                   10                  15      


Leu Cys Leu Leu Leu Asn Ser His Phe Leu Thr Glu Ala Gly Ile His 
            20                  25                  30          


Val Phe Ile Leu Gly Cys Phe Ser Ala Gly Leu Pro Lys Thr Glu Ala 
        35                  40                  45              


Asn Trp Val Asn Val Ile Ser Asp Leu Lys Lys Ile Glu Asp Leu Ile 
    50                  55                  60                  


Gln Ser Met His Ile Asp Ala Thr Leu Tyr Thr Glu Ser Asp Val His 
65                  70                  75                  80  


Pro Ser Cys Lys Val Thr Ala Met Lys Cys Phe Leu Leu Glu Leu Gln 
                85                  90                  95      


Val Ile Ser Leu Glu Ser Gly Asp Ala Ser Ile His Asp Thr Val Glu 
            100                 105                 110         


Asn Leu Ile Ile Leu Ala Asn Asn Ser Leu Ser Ser Asn Gly Asn Val 
        115                 120                 125             


Thr Glu Ser Gly Cys Lys Glu Cys Glu Glu Leu Glu Glu Lys Asn Ile 
    130                 135                 140                 


Lys Glu Phe Leu Gln Ser Phe Val His Ile Val Gln Met Phe Ile Asn 
145                 150                 155                 160 


Thr Ser 
        


<210>  6
<211>  489
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IL-15

<400>  6
atgagaattt cgaaaccaca tttgagaagt atttccatcc agtgctactt gtgtttactt       60

ctaaacagtc attttctaac tgaagctggc attcatgtct tcattttggg ctgtttcagt      120

gcagggcttc ctaaaacaga agccaactgg gtgaatgtaa taagtgattt gaaaaaaatt      180

gaagatctta ttcaatctat gcatattgat gctactttat atacggaaag tgatgttcac      240

cccagttgca aagtaacagc aatgaagtgc tttctcttgg agttacaagt tatttcactt      300

gagtccggag atgcaagtat tcatgataca gtagaaaatc tgatcatcct agcaaacaac      360

agtttgtctt ctaatgggaa tgtaacagaa tctggatgca aagaatgtga ggaactggag      420

gaaaaaaata ttaaagaatt tttgcagagt tttgtacata ttgtccaaat gttcatcaac      480

acttcttga                                                              489


<210>  7
<211>  588
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WPRE sequence

<400>  7
atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc       60

cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta      120

tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt      180

ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg      240

gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta      300

ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt      360

tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg      420

cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca      480

atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc      540

gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgc                   588


<210>  8
<211>  553
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ET promoter

<400>  8
cgcgagttaa taattaccag cgcgggccaa ataaataatc cgcgaggggc aggtgacgtt       60

tgcccagcgc gcgctggtaa ttattaacct cgcgaatatt gattcgaggc cgcgattgcc      120

gcaatcgcga ggggcaggtg acctttgccc agcgcgcgtt cgccccgccc cggacggtat      180

cgataagctt aggagcttgg gctgcaggtc gagggcactg ggaggatgtt gagtaagatg      240

gaaaactact gatgaccctt gcagagacag agtattagga catgtttgaa caggggccgg      300

gcgatcagca ggtagctcta gaggatcccc gtctgtctgc acatttcgta gagcgagtgt      360

tccgatactc taatctccct aggcaaggtt catatttgtg taggttactt attctccttt      420

tgttgactaa gtcaataatc agaatcagca ggtttggagt cagcttggca gggatcagca      480

gcctgggttg gaaggagggg gtataaaagc cccttcacca ggagaagccg tcacacagat      540

ccacaagctc ctg                                                         553


<210>  9
<211>  335
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Albumin promoter

<400>  9
ggcatgcttc catgccaagg cccacactga aatgctcaaa tgggagacaa agagattaag       60

ctcttatgta aaatttgctg ttttacataa ctttaatgaa tggacaaagt cttgtgcatg      120

ggggtggggg tggggttaga ggggaacagc tccagatggc aaacatacgc aagggattta      180

gtcaaacaac tttttggcaa agatggtatg attttgtaat ggggtaggaa ccaatgaaat      240

gcgaggtaag tatggttaat gatctacagt tattggttaa agaagtatat tagagcgagt      300

ctttctgcac acagatcacc tttcctatca acccc                                 335


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-142 target sequence

<400>  10
tccataaagt aggaaacact aca                                               23


<210>  11
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-155 target sequence

<400>  11
cccctatcac gattagcatt aa                                                22


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  miR-223 target sequence

<400>  12
ggggtatttg acaaactgac a                                                 21


