                         SEQUENCE LISTING

<110>  DuPont Nutrition Biosciences ApS
 
<120>  BACTERIUM WITH REDUCED SENSITIVITY TO BACTERIOPHAGE

<130>  NB41696-WO-PCT

<150>  US63/118,315
<151>  2020-11-25

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  2073
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  nucleotide sequence encoding SL12699 autolysin

<400>  1
atgaaactga aaaaaactca cattatttca ctaatactct tttctggatt actatttaca       60

aaaccagtat tagctgaaga cactattagt tcagataaca ccccgatggg gcactacatt      120

gagcaaattc aaactcaaga aacttccaag gacagtagta gtgatacatc atctacaact      180

cctaatatca gagcacgttc atttgcagca gctccaactg ccaatgttcc ctctgatctc      240

aagtccgatg ataatacact gccaagaaaa gatgctgtag atattgctag ctatcaatca      300

tggatgacac aagcagactt taattctctt aagacatctg gtgtcaagtc aatcgttgtt      360

aaactaaccg aaggaactaa ttatacgaac ccatatgctg caaatcaaat taaaatggca      420

cagaatgccg gcttaaatgt tgcagtttat cactacgcac gcttaacagg agccaattca      480

caaagtgatg caaattcatt agccatacag gaagccgcat actttgcaaa agtagctaaa      540

tcttttggat tgtctagcaa cattgtaatg attatggact gcgagcaacc ttatagagat      600

ggctcgggaa atattatcgg acccaatccc ataacggttg attgggcaac agctggtgta      660

caatttgcta acacgctcaa agcaaatggt tatagcaaca caaagtttta tacctctgct      720

tcatggattg gaacagatac agcaacttgt caaatgaact ataatactct aggtggtctc      780

aaaaatcttt gggctgctca atatctatat ggcaagccat cttctagtaa tcttcagaat      840

acgcaatatg gtgcttggca atatacaagt cagatgtatt atcaaggaac ctctaacttg      900

aaagccaatg ctgttgatac ttcaattgat tatagtaatt attttacctc tacatcccct      960

gttcctcctt caacttatac tataacattt aacacagacg gtggaacacc aattgccaat     1020

caatcggttg cagctggtag tgtagtaagc caaccagcag ctcctactaa agctggattt     1080

aacttctcag gttggtatag tgattcttca cttactcaag cttataactt cgcaaaagta     1140

attacatcta atacaaccct ttatgctaaa tggatcccga taacatctcc ttcaatttct     1200

taccaagccc aagtccaaaa tataggatgg caaaaaaatt cctataacgg agaaactgct     1260

ggtacaactg ggttagggtt acgcatggag tcacttaaaa ttagccttat caacctttca     1320

aacggtctga ctaattcaaa tagtcacatt caatatcagg gttatgtaca aaatatcgga     1380

tggcaaaatc cggttcaaga tgggacaatt gctggaacag ttggacaagg gttgcgattt     1440

gaggcaataa aaatgaattt aagcggagaa atagcaaatc aatacgatct atactaccga     1500

gttcaagctc aaaatattgg atggatggat tgggcaaaaa atggcgaagc tgctggaact     1560

tctacgatgt cctaccgttt agaagccatt caaattcaac tcgttaaaaa aggaaatcca     1620

gctcctggag ctacaacttt tacttttttg actactcctt ccctccagta ttgcactcag     1680

gttcaaaaca ttggctggca aaatcctgta tctactggtc agatctctgg aacggtgggt     1740

aaaagcttac gaacggaagc tttaaaaatt aatattggca acctttcagc aggtattgac     1800

ggtggggtat cgtatagcag ccaaattcaa aatattggct ggcaagcacc agtatccaat     1860

ggtcaaatct ctggaactgt aggacaaagt ctacgtttag aggccttgaa aataaacctc     1920

actggtagaa tctcacagta ttttgatatc aattatgcca gccaagtcca aaacatcggc     1980

tggcaagcac cagtatccaa cggtcaaatc tctggaactg tgggaatgtc cttacgagtt     2040

gaagctgtaa aactctctct ttctccaaaa taa                                  2073


<210>  2
<211>  690
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SL12699 autolysin amino acid sequence

<400>  2

Met Lys Leu Lys Lys Thr His Ile Ile Ser Leu Ile Leu Phe Ser Gly 
1               5                   10                  15      


Leu Leu Phe Thr Lys Pro Val Leu Ala Glu Asp Thr Ile Ser Ser Asp 
            20                  25                  30          


Asn Thr Pro Met Gly His Tyr Ile Glu Gln Ile Gln Thr Gln Glu Thr 
        35                  40                  45              


Ser Lys Asp Ser Ser Ser Asp Thr Ser Ser Thr Thr Pro Asn Ile Arg 
    50                  55                  60                  


Ala Arg Ser Phe Ala Ala Ala Pro Thr Ala Asn Val Pro Ser Asp Leu 
65                  70                  75                  80  


Lys Ser Asp Asp Asn Thr Leu Pro Arg Lys Asp Ala Val Asp Ile Ala 
                85                  90                  95      


Ser Tyr Gln Ser Trp Met Thr Gln Ala Asp Phe Asn Ser Leu Lys Thr 
            100                 105                 110         


Ser Gly Val Lys Ser Ile Val Val Lys Leu Thr Glu Gly Thr Asn Tyr 
        115                 120                 125             


Thr Asn Pro Tyr Ala Ala Asn Gln Ile Lys Met Ala Gln Asn Ala Gly 
    130                 135                 140                 


Leu Asn Val Ala Val Tyr His Tyr Ala Arg Leu Thr Gly Ala Asn Ser 
145                 150                 155                 160 


Gln Ser Asp Ala Asn Ser Leu Ala Ile Gln Glu Ala Ala Tyr Phe Ala 
                165                 170                 175     


Lys Val Ala Lys Ser Phe Gly Leu Ser Ser Asn Ile Val Met Ile Met 
            180                 185                 190         


Asp Cys Glu Gln Pro Tyr Arg Asp Gly Ser Gly Asn Ile Ile Gly Pro 
        195                 200                 205             


Asn Pro Ile Thr Val Asp Trp Ala Thr Ala Gly Val Gln Phe Ala Asn 
    210                 215                 220                 


Thr Leu Lys Ala Asn Gly Tyr Ser Asn Thr Lys Phe Tyr Thr Ser Ala 
225                 230                 235                 240 


Ser Trp Ile Gly Thr Asp Thr Ala Thr Cys Gln Met Asn Tyr Asn Thr 
                245                 250                 255     


Leu Gly Gly Leu Lys Asn Leu Trp Ala Ala Gln Tyr Leu Tyr Gly Lys 
            260                 265                 270         


Pro Ser Ser Ser Asn Leu Gln Asn Thr Gln Tyr Gly Ala Trp Gln Tyr 
        275                 280                 285             


Thr Ser Gln Met Tyr Tyr Gln Gly Thr Ser Asn Leu Lys Ala Asn Ala 
    290                 295                 300                 


Val Asp Thr Ser Ile Asp Tyr Ser Asn Tyr Phe Thr Ser Thr Ser Pro 
305                 310                 315                 320 


Val Pro Pro Ser Thr Tyr Thr Ile Thr Phe Asn Thr Asp Gly Gly Thr 
                325                 330                 335     


Pro Ile Ala Asn Gln Ser Val Ala Ala Gly Ser Val Val Ser Gln Pro 
            340                 345                 350         


Ala Ala Pro Thr Lys Ala Gly Phe Asn Phe Ser Gly Trp Tyr Ser Asp 
        355                 360                 365             


Ser Ser Leu Thr Gln Ala Tyr Asn Phe Ala Lys Val Ile Thr Ser Asn 
    370                 375                 380                 


Thr Thr Leu Tyr Ala Lys Trp Ile Pro Ile Thr Ser Pro Ser Ile Ser 
385                 390                 395                 400 


Tyr Gln Ala Gln Val Gln Asn Ile Gly Trp Gln Lys Asn Ser Tyr Asn 
                405                 410                 415     


Gly Glu Thr Ala Gly Thr Thr Gly Leu Gly Leu Arg Met Glu Ser Leu 
            420                 425                 430         


Lys Ile Ser Leu Ile Asn Leu Ser Asn Gly Leu Thr Asn Ser Asn Ser 
        435                 440                 445             


His Ile Gln Tyr Gln Gly Tyr Val Gln Asn Ile Gly Trp Gln Asn Pro 
    450                 455                 460                 


Val Gln Asp Gly Thr Ile Ala Gly Thr Val Gly Gln Gly Leu Arg Phe 
465                 470                 475                 480 


Glu Ala Ile Lys Met Asn Leu Ser Gly Glu Ile Ala Asn Gln Tyr Asp 
                485                 490                 495     


Leu Tyr Tyr Arg Val Gln Ala Gln Asn Ile Gly Trp Met Asp Trp Ala 
            500                 505                 510         


Lys Asn Gly Glu Ala Ala Gly Thr Ser Thr Met Ser Tyr Arg Leu Glu 
        515                 520                 525             


Ala Ile Gln Ile Gln Leu Val Lys Lys Gly Asn Pro Ala Pro Gly Ala 
    530                 535                 540                 


Thr Thr Phe Thr Phe Leu Thr Thr Pro Ser Leu Gln Tyr Cys Thr Gln 
545                 550                 555                 560 


Val Gln Asn Ile Gly Trp Gln Asn Pro Val Ser Thr Gly Gln Ile Ser 
                565                 570                 575     


Gly Thr Val Gly Lys Ser Leu Arg Thr Glu Ala Leu Lys Ile Asn Ile 
            580                 585                 590         


Gly Asn Leu Ser Ala Gly Ile Asp Gly Gly Val Ser Tyr Ser Ser Gln 
        595                 600                 605             


Ile Gln Asn Ile Gly Trp Gln Ala Pro Val Ser Asn Gly Gln Ile Ser 
    610                 615                 620                 


Gly Thr Val Gly Gln Ser Leu Arg Leu Glu Ala Leu Lys Ile Asn Leu 
625                 630                 635                 640 


Thr Gly Arg Ile Ser Gln Tyr Phe Asp Ile Asn Tyr Ala Ser Gln Val 
                645                 650                 655     


Gln Asn Ile Gly Trp Gln Ala Pro Val Ser Asn Gly Gln Ile Ser Gly 
            660                 665                 670         


Thr Val Gly Met Ser Leu Arg Val Glu Ala Val Lys Leu Ser Leu Ser 
        675                 680                 685             


Pro Lys 
    690 


<210>  3
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer CloneAutoF

<400>  3
ttctggaatg ggattcagga ctaccactat tcccaaagca tttc                        44


<210>  4
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer CloneAutoR

<400>  4
atccgtgtcg ttctgtccac gcaatgggag atgattggag agg                         43


<210>  5
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer VF-pg9

<400>  5
gtggacagaa cgacacggat                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer VR-pg9

<400>  6
tcctgaatcc cattccagaa                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer RSF

<400>  7
ttctggaatg ggattcagga                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer GC pG9vF

<400>  8
atccgtgtcg ttctgtccac                                                   20


<210>  9
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer Autolysin_F1

<400>  9
ctcacattat ttcactaata ctcttttctg g                                      31


<210>  10
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer Autolysin_F2

<400>  10
catggattgg aacagataca gcaacttg                                          28


<210>  11
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer Autolysin_F3

<400>  11
caatacgatc tatactaccg agttcaagc                                         29


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer internalErmR-F

<400>  12
gctgaatcga gacttgagtg                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer internalErmR-R

<400>  13
gtcatctatt caacttatcg                                                   20


<210>  14
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer AutolysinNested_F

<400>  14
gccaactttg tcttcatact cacc                                              24


<210>  15
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer AutolysinNested_R

<400>  15
gtatgttttg gaagccctca gg                                                22


