                         SEQUENCE LISTING

<110>  Institute of Medical Biology, Chinese Academy of Medical 
       Sciences & Peking Union Medical College; LU, Shan
 
<120>  Immunity and Protection of SARS-CoV-2 DNA and protein vaccine

<130>  P10962-PCT

<150>  PCT/CN2020/131098
<151>  2020-11-24

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1273
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of S protein

<400>  1

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala 
        675                 680                 685             


Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser 
    690                 695                 700                 


Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile 
705                 710                 715                 720 


Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val 
                725                 730                 735     


Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu 
            740                 745                 750         


Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr 
        755                 760                 765             


Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln 
    770                 775                 780                 


Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe 
785                 790                 795                 800 


Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser 
                805                 810                 815     


Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly 
            820                 825                 830         


Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp 
        835                 840                 845             


Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu 
    850                 855                 860                 


Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly 
865                 870                 875                 880 


Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile 
                885                 890                 895     


Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr 
            900                 905                 910         


Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn 
        915                 920                 925             


Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala 
    930                 935                 940                 


Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn 
945                 950                 955                 960 


Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val 
                965                 970                 975     


Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln 
            980                 985                 990         


Ile Asp Arg Leu Ile Thr Gly Arg  Leu Gln Ser Leu Gln  Thr Tyr Val 
        995                 1000                 1005             


Thr Gln  Gln Leu Ile Arg Ala  Ala Glu Ile Arg Ala  Ser Ala Asn 
    1010                 1015                 1020             


Leu Ala  Ala Thr Lys Met Ser  Glu Cys Val Leu Gly  Gln Ser Lys 
    1025                 1030                 1035             


Arg Val  Asp Phe Cys Gly Lys  Gly Tyr His Leu Met  Ser Phe Pro 
    1040                 1045                 1050             


Gln Ser  Ala Pro His Gly Val  Val Phe Leu His Val  Thr Tyr Val 
    1055                 1060                 1065             


Pro Ala  Gln Glu Lys Asn Phe  Thr Thr Ala Pro Ala  Ile Cys His 
    1070                 1075                 1080             


Asp Gly  Lys Ala His Phe Pro  Arg Glu Gly Val Phe  Val Ser Asn 
    1085                 1090                 1095             


Gly Thr  His Trp Phe Val Thr  Gln Arg Asn Phe Tyr  Glu Pro Gln 
    1100                 1105                 1110             


Ile Ile  Thr Thr Asp Asn Thr  Phe Val Ser Gly Asn  Cys Asp Val 
    1115                 1120                 1125             


Val Ile  Gly Ile Val Asn Asn  Thr Val Tyr Asp Pro  Leu Gln Pro 
    1130                 1135                 1140             


Glu Leu  Asp Ser Phe Lys Glu  Glu Leu Asp Lys Tyr  Phe Lys Asn 
    1145                 1150                 1155             


His Thr  Ser Pro Asp Val Asp  Leu Gly Asp Ile Ser  Gly Ile Asn 
    1160                 1165                 1170             


Ala Ser  Val Val Asn Ile Gln  Lys Glu Ile Asp Arg  Leu Asn Glu 
    1175                 1180                 1185             


Val Ala  Lys Asn Leu Asn Glu  Ser Leu Ile Asp Leu  Gln Glu Leu 
    1190                 1195                 1200             


Gly Lys  Tyr Glu Gln Tyr Ile  Lys Trp Pro Trp Tyr  Ile Trp Leu 
    1205                 1210                 1215             


Gly Phe  Ile Ala Gly Leu Ile  Ala Ile Val Met Val  Thr Ile Met 
    1220                 1225                 1230             


Leu Cys  Cys Met Thr Ser Cys  Cys Ser Cys Leu Lys  Gly Cys Cys 
    1235                 1240                 1245             


Ser Cys  Gly Ser Cys Cys Lys  Phe Asp Glu Asp Asp  Ser Glu Pro 
    1250                 1255                 1260             


Val Leu  Lys Gly Val Lys Leu  His Tyr Thr 
    1265                 1270             


<210>  2
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S protein coding sequence, S-FL-wt

<400>  2
atgtttgttt ttcttgtttt attgccacta gtctctagtc agtgtgttaa tcttacaacc       60

agaactcaat taccccctgc atacactaat tctttcacac gtggtgttta ttaccctgac      120

aaagttttca gatcctcagt tttacattca actcaggact tgttcttacc tttcttttcc      180

aatgttactt ggttccatgc tatacatgtc tctgggacca atggtactaa gaggtttgat      240

aaccctgtcc taccatttaa tgatggtgtt tattttgctt ccactgagaa gtctaacata      300

ataagaggct ggatttttgg tactacttta gattcgaaga cccagtccct acttattgtt      360

aataacgcta ctaatgttgt tattaaagtc tgtgaatttc aattttgtaa tgatccattt      420

ttgggtgttt attaccacaa aaacaacaaa agttggatgg aaagtgagtt cagagtttat      480

tctagtgcga ataattgcac ttttgaatat gtctctcagc cttttcttat ggaccttgaa      540

ggaaaacagg gtaatttcaa aaatcttagg gaatttgtgt ttaagaatat tgatggttat      600

tttaaaatat attctaagca cacgcctatt aatttagtgc gtgatctccc tcagggtttt      660

tcggctttag aaccattggt agatttgcca ataggtatta acatcactag gtttcaaact      720

ttacttgctt tacatagaag ttatttgact cctggtgatt cttcttcagg ttggacagct      780

ggtgctgcag cttattatgt gggttatctt caacctagga cttttctatt aaaatataat      840

gaaaatggaa ccattacaga tgctgtagac tgtgcacttg accctctctc agaaacaaag      900

tgtacgttga aatccttcac tgtagaaaaa ggaatctatc aaacttctaa ctttagagtc      960

caaccaacag aatctattgt tagatttcct aatattacaa acttgtgccc ttttggtgaa     1020

gtttttaacg ccaccagatt tgcatctgtt tatgcttgga acaggaagag aatcagcaac     1080

tgtgttgctg attattctgt cctatataat tccgcatcat tttccacttt taagtgttat     1140

ggagtgtctc ctactaaatt aaatgatctc tgctttacta atgtctatgc agattcattt     1200

gtaattagag gtgatgaagt cagacaaatc gctccagggc aaactggaaa gattgctgat     1260

tataattata aattaccaga tgattttaca ggctgcgtta tagcttggaa ttctaacaat     1320

cttgattcta aggttggtgg taattataat tacctgtata gattgtttag gaagtctaat     1380

ctcaaacctt ttgagagaga tatttcaact gaaatctatc aggccggtag cacaccttgt     1440

aatggtgttg aaggttttaa ttgttacttt cctttacaat catatggttt ccaacccact     1500

aatggtgttg gttaccaacc atacagagta gtagtacttt cttttgaact tctacatgca     1560

ccagcaactg tttgtggacc taaaaagtct actaatttgg ttaaaaacaa atgtgtcaat     1620

ttcaacttca atggtttaac aggcacaggt gttcttactg agtctaacaa aaagtttctg     1680

cctttccaac aatttggcag agacattgct gacactactg atgctgtccg tgatccacag     1740

acacttgaga ttcttgacat tacaccatgt tcttttggtg gtgtcagtgt tataacacca     1800

ggaacaaata cttctaacca ggttgctgtt ctttatcagg atgttaactg cacagaagtc     1860

cctgttgcta ttcatgcaga tcaacttact cctacttggc gtgtttattc tacaggttct     1920

aatgtttttc aaacacgtgc aggctgttta ataggggctg aacatgtcaa caactcatat     1980

gagtgtgaca tacccattgg tgcaggtata tgcgctagtt atcagactca gactaattct     2040

cctcggcggg cacgtagtgt agctagtcaa tccatcattg cctacactat gtcacttggt     2100

gcagaaaatt cagttgctta ctctaataac tctattgcca tacccacaaa ttttactatt     2160

agtgttacca cagaaattct accagtgtct atgaccaaga catcagtaga ttgtacaatg     2220

tacatttgtg gtgattcaac tgaatgcagc aatcttttgt tgcaatatgg cagtttttgt     2280

acacaattaa accgtgcttt aactggaata gctgttgaac aagacaaaaa cacccaagaa     2340

gtttttgcac aagtcaaaca aatttacaaa acaccaccaa ttaaagattt tggtggtttt     2400

aatttttcac aaatattacc agatccatca aaaccaagca agaggtcatt tattgaagat     2460

ctacttttca acaaagtgac acttgcagat gctggcttca tcaaacaata tggtgattgc     2520

cttggtgata ttgctgctag agacctcatt tgtgcacaaa agtttaacgg ccttactgtt     2580

ttgccacctt tgctcacaga tgaaatgatt gctcaataca cttctgcact gttagcgggt     2640

acaatcactt ctggttggac ctttggtgca ggtgctgcat tacaaatacc atttgctatg     2700

caaatggctt ataggtttaa tggtattgga gttacacaga atgttctcta tgagaaccaa     2760

aaattgattg ccaaccaatt taatagtgct attggcaaaa ttcaagactc actttcttcc     2820

acagcaagtg cacttggaaa acttcaagat gtggtcaacc aaaatgcaca agctttaaac     2880

acgcttgtta aacaacttag ctccaatttt ggtgcaattt caagtgtttt aaatgatatc     2940

ctttcacgtc ttgacaaagt tgaggctgaa gtgcaaattg ataggttgat cacaggcaga     3000

cttcaaagtt tgcagacata tgtgactcaa caattaatta gagctgcaga aatcagagct     3060

tctgctaatc ttgctgctac taaaatgtca gagtgtgtac ttggacaatc aaaaagagtt     3120

gatttttgtg gaaagggcta tcatcttatg tccttccctc agtcagcacc tcatggtgta     3180

gtcttcttgc atgtgactta tgtccctgca caagaaaaga acttcacaac tgctcctgcc     3240

atttgtcatg atggaaaagc acactttcct cgtgaaggtg tctttgtttc aaatggcaca     3300

cactggtttg taacacaaag gaatttttat gaaccacaaa tcattactac agacaacaca     3360

tttgtgtctg gtaactgtga tgttgtaata ggaattgtca acaacacagt ttatgatcct     3420

ttgcaacctg aattagactc attcaaggag gagttagata aatattttaa gaatcataca     3480

tcaccagatg ttgatttagg tgacatctct ggcattaatg cttcagttgt aaacattcaa     3540

aaagaaattg accgcctcaa tgaggttgcc aagaatttaa atgaatctct catcgatctc     3600

caagaacttg gaaagtatga gcagtatata aaatggccat ggtacatttg gctaggtttt     3660

atagctggct tgattgccat agtaatggtg acaattatgc tttgctgtat gaccagttgc     3720

tgtagttgtc tcaagggctg ttgttcttgt ggatcctgct gcaaatttga tgaagacgac     3780

tctgagccag tgctcaaagg agtcaaatta cattacacat aa                        3822


<210>  3
<211>  3822
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S protein coding sequence, S-FL-opt

<400>  3
atgttcgtgt tcctggtgct cctccctctc gtgtcttctc agtgcgtgaa cctgaccaca       60

cggacccagc tgccacccgc ttacaccaac tccttcacaa gaggcgtgta ctaccccgac      120

aaggtgttcc ggtcttctgt gctccactct acccaggacc tgttcctgcc attcttctct      180

aacgtgacat ggttccacgc tatccacgtg tctggcacca acggcacaaa gagattcgac      240

aaccccgtgc tcccattcaa cgacggcgtg tacttcgcta gcacagagaa gtccaacatc      300

atccggggct ggatcttcgg caccacactg gactctaaga cccagtccct cctcatcgtg      360

aacaacgcca caaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgacccattc      420

ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt ccgggtgtac      480

tctagcgcta acaactgcac attcgagtac gtgtctcagc cattcctcat ggacctcgag      540

ggcaagcagg gcaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actctaagca caccccaatc aacctggtga gggacctgcc tcagggcttc      660

tccgctctcg agccactggt ggacctgcca atcggcatca acatcacccg gttccagaca      720

ctcctcgccc tccaccggtc ttacctgacc ccaggcgact ctagcagcgg ctggaccgcc      780

ggcgccgccg cttactacgt gggctacctg cagcctagga cattcctcct gaagtacaac      840

gagaacggca caatcaccga cgctgtggac tgcgccctcg acccactgag cgagacaaag      900

tgcacactga agtctttcac agtggagaag ggcatctacc agacatctaa cttcagagtg      960

cagcctacag agtctatcgt gagattccca aacatcacaa acctgtgccc tttcggcgag     1020

gtgttcaacg ctacacggtt cgcttctgtg tacgcttgga accggaagcg gatctctaac     1080

tgcgtggccg actactctgt gctgtacaac tccgcctctt tctccacatt caagtgctac     1140

ggcgtgagcc caacaaagct gaacgacctg tgcttcacaa acgtgtacgc cgactctttc     1200

gtgatccggg gcgacgaggt gaggcagatc gctccaggcc agacaggcaa gatcgccgac     1260

tacaactaca agctccccga cgacttcaca ggctgcgtga tcgcttggaa ctctaacaac     1320

ctggactcta aggtgggcgg caactacaac tacctgtaca gactgttccg gaagtctaac     1380

ctgaagcctt tcgagcggga catctctacc gagatctacc aggccggcag caccccttgc     1440

aacggcgtgg agggcttcaa ctgctacttc ccactgcagt cttacggctt ccagcctaca     1500

aacggcgtgg gctaccagcc ataccgggtg gtggtgctgt ccttcgagct cctccacgcc     1560

cccgctacag tgtgcggccc aaagaagtcc acaaacctcg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac aggcacaggc gtgctcacag agtctaacaa gaagttcctc     1680

cctttccagc agttcggccg ggacatcgcc gacaccaccg acgccgtgag ggaccctcag     1740

acactcgaga tcctcgacat caccccttgc tctttcggcg gcgtgtctgt gatcaccccc     1800

ggcacaaaca catctaacca ggtggctgtg ctgtaccagg acgtgaactg caccgaggtg     1860

ccagtggcta tccacgccga ccagctcacc cctacatgga gggtgtacag cacaggctct     1920

aacgtgttcc agacgagggc cggctgcctc atcggcgccg agcacgtgaa caactcttac     1980

gagtgcgaca tcccaatcgg cgctggcatc tgcgcttctt accagaccca gacaaacagc     2040

cctaggagag ctaggtccgt ggctagccag tccatcatcg cttacaccat gtctctgggc     2100

gctgagaact ctgtggctta ctctaacaac tctatcgcta tccctacaaa cttcacaatc     2160

tctgtgacca ccgagatcct gccagtgtct atgacaaaga catccgtgga ctgcaccatg     2220

tacatctgcg gcgacagcac cgagtgcagc aacctgctcc tgcagtacgg ctctttctgc     2280

acacagctga accgggccct caccggcatc gccgtggagc aggacaagaa cacccaggag     2340

gtgttcgctc aggtgaagca gatctacaag acccccccta tcaaggactt cggcggcttc     2400

aacttctctc agatcctccc cgaccctagc aagcctagca agcggtcttt catcgaggac     2460

ctcctgttca acaaggtgac actcgctgac gctggcttca tcaagcagta cggcgactgc     2520

ctgggcgaca tcgccgctag agacctgatc tgcgctcaga agttcaacgg cctcacagtg     2580

ctgcccccac tcctgaccga cgagatgatc gctcagtaca ccagcgccct cctcgctggc     2640

acaatcacat ctggctggac attcggcgcc ggcgccgccc tgcagatccc attcgccatg     2700

cagatggctt accggttcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactccgct atcggcaaga tccaggactc cctgtctagc     2820

accgctagcg ccctgggcaa gctccaggac gtggtgaacc agaacgccca ggccctgaac     2880

acactggtga agcagctgtc ttctaacttc ggcgctatca gctctgtgct caacgacatc     2940

ctgtctagac tcgacaaggt ggaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagtccc tccagacata cgtgacccag cagctcatcc gggccgctga gatcagagct     3060

agcgctaacc tcgccgctac caagatgagc gagtgcgtgc tgggccagtc taagcgggtg     3120

gacttctgcg gcaagggcta ccacctcatg tctttccctc agtccgcccc tcacggcgtg     3180

gtgttcctcc acgtgacata cgtgccagct caggagaaga acttcaccac cgccccagct     3240

atctgccacg acggcaaggc ccacttccca cgggagggcg tgttcgtgtc taacggcacc     3300

cactggttcg tgacacagcg gaacttctac gagcctcaga tcatcaccac agacaacaca     3360

ttcgtgtccg gcaactgcga cgtggtgatc ggcatcgtga acaacacagt gtacgaccca     3420

ctgcagcccg agctcgactc tttcaaggag gagctcgaca agtacttcaa gaaccacacc     3480

agccccgacg tggacctggg cgacatctct ggcatcaacg cctctgtggt gaacatccag     3540

aaggagatcg acagactgaa cgaggtggct aagaacctga acgagtccct gatcgacctg     3600

caggagctgg gcaagtacga gcagtacatc aagtggcctt ggtacatctg gctgggcttc     3660

atcgctggcc tgatcgctat cgtgatggtg acaatcatgc tgtgctgcat gacctcttgc     3720

tgctcttgcc tgaagggctg ctgctcttgc ggctcttgct gcaagttcga cgaggacgac     3780

tccgagcccg tgctgaaggg cgtgaagctc cactacacat ga                        3822


<210>  4
<211>  3642
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  truncated S protein coding sequence, S-dTM-opt

<400>  4
atgttcgtgt tcctggtgct cctccctctc gtgtcttctc agtgcgtgaa cctgaccaca       60

cggacccagc tgccacccgc ttacaccaac tccttcacaa gaggcgtgta ctaccccgac      120

aaggtgttcc ggtcttctgt gctccactct acccaggacc tgttcctgcc attcttctct      180

aacgtgacat ggttccacgc tatccacgtg tctggcacca acggcacaaa gagattcgac      240

aaccccgtgc tcccattcaa cgacggcgtg tacttcgcta gcacagagaa gtccaacatc      300

atccggggct ggatcttcgg caccacactg gactctaaga cccagtccct cctcatcgtg      360

aacaacgcca caaacgtggt gatcaaggtg tgcgagttcc agttctgcaa cgacccattc      420

ctgggcgtgt actaccacaa gaacaacaag tcttggatgg agagcgagtt ccgggtgtac      480

tctagcgcta acaactgcac attcgagtac gtgtctcagc cattcctcat ggacctcgag      540

ggcaagcagg gcaacttcaa gaacctgagg gagttcgtgt tcaagaacat cgacggctac      600

ttcaagatct actctaagca caccccaatc aacctggtga gggacctgcc tcagggcttc      660

tccgctctcg agccactggt ggacctgcca atcggcatca acatcacccg gttccagaca      720

ctcctcgccc tccaccggtc ttacctgacc ccaggcgact ctagcagcgg ctggaccgcc      780

ggcgccgccg cttactacgt gggctacctg cagcctagga cattcctcct gaagtacaac      840

gagaacggca caatcaccga cgctgtggac tgcgccctcg acccactgag cgagacaaag      900

tgcacactga agtctttcac agtggagaag ggcatctacc agacatctaa cttcagagtg      960

cagcctacag agtctatcgt gagattccca aacatcacaa acctgtgccc tttcggcgag     1020

gtgttcaacg ctacacggtt cgcttctgtg tacgcttgga accggaagcg gatctctaac     1080

tgcgtggccg actactctgt gctgtacaac tccgcctctt tctccacatt caagtgctac     1140

ggcgtgagcc caacaaagct gaacgacctg tgcttcacaa acgtgtacgc cgactctttc     1200

gtgatccggg gcgacgaggt gaggcagatc gctccaggcc agacaggcaa gatcgccgac     1260

tacaactaca agctccccga cgacttcaca ggctgcgtga tcgcttggaa ctctaacaac     1320

ctggactcta aggtgggcgg caactacaac tacctgtaca gactgttccg gaagtctaac     1380

ctgaagcctt tcgagcggga catctctacc gagatctacc aggccggcag caccccttgc     1440

aacggcgtgg agggcttcaa ctgctacttc ccactgcagt cttacggctt ccagcctaca     1500

aacggcgtgg gctaccagcc ataccgggtg gtggtgctgt ccttcgagct cctccacgcc     1560

cccgctacag tgtgcggccc aaagaagtcc acaaacctcg tgaagaacaa gtgcgtgaac     1620

ttcaacttca acggcctgac aggcacaggc gtgctcacag agtctaacaa gaagttcctc     1680

cctttccagc agttcggccg ggacatcgcc gacaccaccg acgccgtgag ggaccctcag     1740

acactcgaga tcctcgacat caccccttgc tctttcggcg gcgtgtctgt gatcaccccc     1800

ggcacaaaca catctaacca ggtggctgtg ctgtaccagg acgtgaactg caccgaggtg     1860

ccagtggcta tccacgccga ccagctcacc cctacatgga gggtgtacag cacaggctct     1920

aacgtgttcc agacgagggc cggctgcctc atcggcgccg agcacgtgaa caactcttac     1980

gagtgcgaca tcccaatcgg cgctggcatc tgcgcttctt accagaccca gacaaacagc     2040

cctaggagag ctaggtccgt ggctagccag tccatcatcg cttacaccat gtctctgggc     2100

gctgagaact ctgtggctta ctctaacaac tctatcgcta tccctacaaa cttcacaatc     2160

tctgtgacca ccgagatcct gccagtgtct atgacaaaga catccgtgga ctgcaccatg     2220

tacatctgcg gcgacagcac cgagtgcagc aacctgctcc tgcagtacgg ctctttctgc     2280

acacagctga accgggccct caccggcatc gccgtggagc aggacaagaa cacccaggag     2340

gtgttcgctc aggtgaagca gatctacaag acccccccta tcaaggactt cggcggcttc     2400

aacttctctc agatcctccc cgaccctagc aagcctagca agcggtcttt catcgaggac     2460

ctcctgttca acaaggtgac actcgctgac gctggcttca tcaagcagta cggcgactgc     2520

ctgggcgaca tcgccgctag agacctgatc tgcgctcaga agttcaacgg cctcacagtg     2580

ctgcccccac tcctgaccga cgagatgatc gctcagtaca ccagcgccct cctcgctggc     2640

acaatcacat ctggctggac attcggcgcc ggcgccgccc tgcagatccc attcgccatg     2700

cagatggctt accggttcaa cggcatcggc gtgacccaga acgtgctgta cgagaaccag     2760

aagctgatcg ctaaccagtt caactccgct atcggcaaga tccaggactc cctgtctagc     2820

accgctagcg ccctgggcaa gctccaggac gtggtgaacc agaacgccca ggccctgaac     2880

acactggtga agcagctgtc ttctaacttc ggcgctatca gctctgtgct caacgacatc     2940

ctgtctagac tcgacaaggt ggaggccgag gtgcagatcg acagactgat cacaggcaga     3000

ctgcagtccc tccagacata cgtgacccag cagctcatcc gggccgctga gatcagagct     3060

agcgctaacc tcgccgctac caagatgagc gagtgcgtgc tgggccagtc taagcgggtg     3120

gacttctgcg gcaagggcta ccacctcatg tctttccctc agtccgcccc tcacggcgtg     3180

gtgttcctcc acgtgacata cgtgccagct caggagaaga acttcaccac cgccccagct     3240

atctgccacg acggcaaggc ccacttccca cgggagggcg tgttcgtgtc taacggcacc     3300

cactggttcg tgacacagcg gaacttctac gagcctcaga tcatcaccac agacaacaca     3360

ttcgtgtccg gcaactgcga cgtggtgatc ggcatcgtga acaacacagt gtacgaccca     3420

ctgcagcccg agctcgactc tttcaaggag gagctcgaca agtacttcaa gaaccacacc     3480

agccccgacg tggacctggg cgacatctct ggcatcaacg cctctgtggt gaacatccag     3540

aaggagatcg acagactgaa cgaggtggct aagaacctga acgagtccct gatcgacctg     3600

caggagctgg gcaagtacga gcagtacatc aagtggcctt ga                        3642


<210>  5
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligmer for amplification of S-FL-opt insert

<400>  5
tccatgggtc ttttctgcag tcaccgtcca agcttgcaat cgccaccatg ttcgtgttcc       60

t                                                                       61


<210>  6
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  oligmer for amplification of S-FL-opt insert

<400>  6
gggattgcga ggatccttat catgtgtagt ggagcttcac g                           41


<210>  7
<211>  685
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of S1 subunit

<400>  7

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val 
1               5                   10                  15      


Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe 
            20                  25                  30          


Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu 
        35                  40                  45              


His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp 
    50                  55                  60                  


Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp 
65                  70                  75                  80  


Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu 
                85                  90                  95      


Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser 
            100                 105                 110         


Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile 
        115                 120                 125             


Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr 
    130                 135                 140                 


Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr 
145                 150                 155                 160 


Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu 
                165                 170                 175     


Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe 
            180                 185                 190         


Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr 
        195                 200                 205             


Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu 
    210                 215                 220                 


Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr 
225                 230                 235                 240 


Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser 
                245                 250                 255     


Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro 
            260                 265                 270         


Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala 
        275                 280                 285             


Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys 
    290                 295                 300                 


Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val 
305                 310                 315                 320 


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
                325                 330                 335     


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
            340                 345                 350         


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
        355                 360                 365             


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
    370                 375                 380                 


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
385                 390                 395                 400 


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
                405                 410                 415     


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
            420                 425                 430         


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
        435                 440                 445             


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
    450                 455                 460                 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
465                 470                 475                 480 


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
                485                 490                 495     


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
            500                 505                 510         


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
        515                 520                 525             


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
    530                 535                 540                 


Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu 
545                 550                 555                 560 


Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val 
                565                 570                 575     


Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe 
            580                 585                 590         


Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val 
        595                 600                 605             


Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala Ile 
    610                 615                 620                 


His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser 
625                 630                 635                 640 


Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val 
                645                 650                 655     


Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala 
            660                 665                 670         


Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg 
        675                 680                 685 


<210>  8
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe for SARS-CoV2 N (nucleocapsid) gene

<400>  8
ttgctgcttg acagatt                                                      17


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  forward primer for SARS-CoV2 N (nucleocapsid) gene

<400>  9
ggggaacttc tcctgctaga at                                                22


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse primer for SARS-CoV2 N (nucleocapsid) gene

<400>  10
cagacatttt gctctcaagc tg                                                22


<210>  11
<211>  8515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCW1093 full length sequence

<400>  11
ctgacgtcgc ggtcgacaat attggctatt ggccattgca tacgttgtat ctatatcata       60

atatgtacat ttatattggc tcatgtccaa tatgaccgcc atgttgacat tagttattga      120

ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc      180

gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat      240

tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc      300

aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc      360

caagtccgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt      420

acatgacctt acgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta      480

ccatggtgat gcggttttgg cagtacacca atgggcgtgg atagcggttt gactcacggg      540

gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac      600

gggactttcc aaaatgtcgt aataaccccg ccccgttgac gcaaatgggc ggtaggcgtg      660

tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac      720

gccatccacg ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc      780

gggaacggtg cattggaacg cggattcccc gtgccaagag tgacgtaagt accgcctata      840

gactctatag gcacacccct ttggctctta tgcatgctat actgtttttg gcttggggcc      900

tatacacccc cgcttcctta tgctataggt gatggtatag cttagcctat aggtgtgggt      960

tattgaccat tattgaccac tcccctattg gtgacgatac tttccattac taatccataa     1020

catggctctt tgccacaact atctctattg gctatatgcc aatactctgt ccttcagaga     1080

ctgacacgga ctctgtattt ttacaggatg gggtcccatt tattatttac aaattcacat     1140

atacaacaac gccgtccccc gtgcccgcag tttttattaa acatagcgtg ggatctccac     1200

gcgaatctcg ggtacgtgtt ccggacatgg gctcttctcc ggtagcggcg gagcttccac     1260

atccgagccc tggtcccatg cctccagcgg ctcatggtcg ctcggcagct ccttgctcct     1320

aacagtggag gccagactta ggcacagcac aatgcccacc accaccagtg tgccgcacaa     1380

ggccgtggcg gtagggtatg tgtctgaaaa tgagctcgga gattgggctc gcaccgctga     1440

cgcagatgga agacttaagg cagcggcaga agaagatgca ggcagctgag ttgttgtatt     1500

ctgataagag tcagaggtaa ctcccgttgc ggtgctgtta acggtggagg gcagtgtagt     1560

ctgagcagta ctcgttgctg ccgcgcgcgc caccagacat aatagctgac agactaacag     1620

actgttcctt tccatgggtc ttttctgcag tcaccgtcca agcttgcaat cgccaccatg     1680

ttcgtgttcc tggtgctcct ccctctcgtg tcttctcagt gcgtgaacct gaccacacgg     1740

acccagctgc cacccgctta caccaactcc ttcacaagag gcgtgtacta ccccgacaag     1800

gtgttccggt cttctgtgct ccactctacc caggacctgt tcctgccatt cttctctaac     1860

gtgacatggt tccacgctat ccacgtgtct ggcaccaacg gcacaaagag attcgacaac     1920

cccgtgctcc cattcaacga cggcgtgtac ttcgctagca cagagaagtc caacatcatc     1980

cggggctgga tcttcggcac cacactggac tctaagaccc agtccctcct catcgtgaac     2040

aacgccacaa acgtggtgat caaggtgtgc gagttccagt tctgcaacga cccattcctg     2100

ggcgtgtact accacaagaa caacaagtct tggatggaga gcgagttccg ggtgtactct     2160

agcgctaaca actgcacatt cgagtacgtg tctcagccat tcctcatgga cctcgagggc     2220

aagcagggca acttcaagaa cctgagggag ttcgtgttca agaacatcga cggctacttc     2280

aagatctact ctaagcacac cccaatcaac ctggtgaggg acctgcctca gggcttctcc     2340

gctctcgagc cactggtgga cctgccaatc ggcatcaaca tcacccggtt ccagacactc     2400

ctcgccctcc accggtctta cctgacccca ggcgactcta gcagcggctg gaccgccggc     2460

gccgccgctt actacgtggg ctacctgcag cctaggacat tcctcctgaa gtacaacgag     2520

aacggcacaa tcaccgacgc tgtggactgc gccctcgacc cactgagcga gacaaagtgc     2580

acactgaagt ctttcacagt ggagaagggc atctaccaga catctaactt cagagtgcag     2640

cctacagagt ctatcgtgag attcccaaac atcacaaacc tgtgcccttt cggcgaggtg     2700

ttcaacgcta cacggttcgc ttctgtgtac gcttggaacc ggaagcggat ctctaactgc     2760

gtggccgact actctgtgct gtacaactcc gcctctttct ccacattcaa gtgctacggc     2820

gtgagcccaa caaagctgaa cgacctgtgc ttcacaaacg tgtacgccga ctctttcgtg     2880

atccggggcg acgaggtgag gcagatcgct ccaggccaga caggcaagat cgccgactac     2940

aactacaagc tccccgacga cttcacaggc tgcgtgatcg cttggaactc taacaacctg     3000

gactctaagg tgggcggcaa ctacaactac ctgtacagac tgttccggaa gtctaacctg     3060

aagcctttcg agcgggacat ctctaccgag atctaccagg ccggcagcac cccttgcaac     3120

ggcgtggagg gcttcaactg ctacttccca ctgcagtctt acggcttcca gcctacaaac     3180

ggcgtgggct accagccata ccgggtggtg gtgctgtcct tcgagctcct ccacgccccc     3240

gctacagtgt gcggcccaaa gaagtccaca aacctcgtga agaacaagtg cgtgaacttc     3300

aacttcaacg gcctgacagg cacaggcgtg ctcacagagt ctaacaagaa gttcctccct     3360

ttccagcagt tcggccggga catcgccgac accaccgacg ccgtgaggga ccctcagaca     3420

ctcgagatcc tcgacatcac cccttgctct ttcggcggcg tgtctgtgat cacccccggc     3480

acaaacacat ctaaccaggt ggctgtgctg taccaggacg tgaactgcac cgaggtgcca     3540

gtggctatcc acgccgacca gctcacccct acatggaggg tgtacagcac aggctctaac     3600

gtgttccaga cgagggccgg ctgcctcatc ggcgccgagc acgtgaacaa ctcttacgag     3660

tgcgacatcc caatcggcgc tggcatctgc gcttcttacc agacccagac aaacagccct     3720

aggagagcta ggtccgtggc tagccagtcc atcatcgctt acaccatgtc tctgggcgct     3780

gagaactctg tggcttactc taacaactct atcgctatcc ctacaaactt cacaatctct     3840

gtgaccaccg agatcctgcc agtgtctatg acaaagacat ccgtggactg caccatgtac     3900

atctgcggcg acagcaccga gtgcagcaac ctgctcctgc agtacggctc tttctgcaca     3960

cagctgaacc gggccctcac cggcatcgcc gtggagcagg acaagaacac ccaggaggtg     4020

ttcgctcagg tgaagcagat ctacaagacc ccccctatca aggacttcgg cggcttcaac     4080

ttctctcaga tcctccccga ccctagcaag cctagcaagc ggtctttcat cgaggacctc     4140

ctgttcaaca aggtgacact cgctgacgct ggcttcatca agcagtacgg cgactgcctg     4200

ggcgacatcg ccgctagaga cctgatctgc gctcagaagt tcaacggcct cacagtgctg     4260

cccccactcc tgaccgacga gatgatcgct cagtacacca gcgccctcct cgctggcaca     4320

atcacatctg gctggacatt cggcgccggc gccgccctgc agatcccatt cgccatgcag     4380

atggcttacc ggttcaacgg catcggcgtg acccagaacg tgctgtacga gaaccagaag     4440

ctgatcgcta accagttcaa ctccgctatc ggcaagatcc aggactccct gtctagcacc     4500

gctagcgccc tgggcaagct ccaggacgtg gtgaaccaga acgcccaggc cctgaacaca     4560

ctggtgaagc agctgtcttc taacttcggc gctatcagct ctgtgctcaa cgacatcctg     4620

tctagactcg acaaggtgga ggccgaggtg cagatcgaca gactgatcac aggcagactg     4680

cagtccctcc agacatacgt gacccagcag ctcatccggg ccgctgagat cagagctagc     4740

gctaacctcg ccgctaccaa gatgagcgag tgcgtgctgg gccagtctaa gcgggtggac     4800

ttctgcggca agggctacca cctcatgtct ttccctcagt ccgcccctca cggcgtggtg     4860

ttcctccacg tgacatacgt gccagctcag gagaagaact tcaccaccgc cccagctatc     4920

tgccacgacg gcaaggccca cttcccacgg gagggcgtgt tcgtgtctaa cggcacccac     4980

tggttcgtga cacagcggaa cttctacgag cctcagatca tcaccacaga caacacattc     5040

gtgtccggca actgcgacgt ggtgatcggc atcgtgaaca acacagtgta cgacccactg     5100

cagcccgagc tcgactcttt caaggaggag ctcgacaagt acttcaagaa ccacaccagc     5160

cccgacgtgg acctgggcga catctctggc atcaacgcct ctgtggtgaa catccagaag     5220

gagatcgaca gactgaacga ggtggctaag aacctgaacg agtccctgat cgacctgcag     5280

gagctgggca agtacgagca gtacatcaag tggccttggt acatctggct gggcttcatc     5340

gctggcctga tcgctatcgt gatggtgaca atcatgctgt gctgcatgac ctcttgctgc     5400

tcttgcctga agggctgctg ctcttgcggc tcttgctgca agttcgacga ggacgactcc     5460

gagcccgtgc tgaagggcgt gaagctccac tacacatgat aaggatcctc gcaatcccta     5520

ggaggattag gcaagggctt gagctcacgc tcttgtgagg gacagaaata caatcagggg     5580

cagtatatga atactccatg gagaaaccca gatctacgta tgatcagcct cgactgtgcc     5640

ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg     5700

tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag     5760

gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga     5820

caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag     5880

ctggggctcg acagctcgag ctaggaccgc tatcaggaca tagcgttggc tacccgtgat     5940

attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc     6000

gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga     6060

ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt     6120

ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga     6180

tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg     6240

cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt     6300

tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga     6360

tcgcggccgc gatccgtcga gagcttggcg taatcatggt catagctgtt tcctgtgtga     6420

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     6480

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     6540

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     6600

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     6660

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     6720

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     6780

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     6840

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     6900

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     6960

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     7020

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     7080

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     7140

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     7200

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     7260

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     7320

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     7380

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     7440

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     7500

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     7560

ctagcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta     7620

tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag     7680

ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata     7740

caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg     7800

acgactgaat ccggtgagaa tggcaaaagc ttatgcattt ctttccagac ttgttcaaca     7860

ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt     7920

gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt acaaacagga     7980

atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca     8040

ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat     8100

gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc     8160

cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc     8220

agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc     8280

ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat     8340

cgcggcctcg agcaagacgt ttcccgttga atatggctca tgctagaact cttccttttt     8400

caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt     8460

atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac          8515


