                         SEQUENCE LISTING

<110>  WOJTOVICH, Andrew et al.
 
<120>  FUSION PROTEIN TARGETING MITOCHONDRIA, METHOD OF MAKING AND USE 
       THEREOF

<130>  1134-058 PCT

<150>  US 63/115,826
<151>  2020-11-19

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  758
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(758)
<223>  Human IMMT (UniProtKB - Q16891)

<400>  1

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Ala Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Gly Ser Ser Gly Leu Thr Thr Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Glu Met Val Leu Gly Pro Ala Ala Tyr Asn 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Ser Ile Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Lys Glu Ser Lys Gln Pro Ala Ser Gln Leu 
        115                 120                 125             


Gln Lys Gln Lys Gly Asp Thr Pro Ala Ser Ala Thr Ala Pro Thr Glu 
    130                 135                 140                 


Ala Ala Gln Ile Ile Ser Ala Ala Gly Asp Thr Leu Ser Val Pro Ala 
145                 150                 155                 160 


Pro Ala Val Gln Pro Glu Glu Ser Leu Lys Thr Asp His Pro Glu Ile 
                165                 170                 175     


Gly Glu Gly Lys Pro Thr Pro Ala Leu Ser Glu Glu Ala Ser Ser Ser 
            180                 185                 190         


Ser Ile Arg Glu Arg Pro Pro Glu Glu Val Ala Ala Arg Leu Ala Gln 
        195                 200                 205             


Gln Glu Lys Gln Glu Gln Val Lys Ile Glu Ser Leu Ala Lys Ser Leu 
    210                 215                 220                 


Glu Asp Ala Leu Arg Gln Thr Ala Ser Val Thr Leu Gln Ala Ile Ala 
225                 230                 235                 240 


Ala Gln Asn Ala Ala Val Gln Ala Val Asn Ala His Ser Asn Ile Leu 
                245                 250                 255     


Lys Ala Ala Met Asp Asn Ser Glu Ile Ala Gly Glu Lys Lys Ser Ala 
            260                 265                 270         


Gln Trp Arg Thr Val Glu Gly Ala Leu Lys Glu Arg Arg Lys Ala Val 
        275                 280                 285             


Asp Glu Ala Ala Asp Ala Leu Leu Lys Ala Lys Glu Glu Leu Glu Lys 
    290                 295                 300                 


Met Lys Ser Val Ile Glu Asn Ala Lys Lys Lys Glu Val Ala Gly Ala 
305                 310                 315                 320 


Lys Pro His Ile Thr Ala Ala Glu Gly Lys Leu His Asn Met Ile Val 
                325                 330                 335     


Asp Leu Asp Asn Val Val Lys Lys Val Gln Ala Ala Gln Ser Glu Ala 
            340                 345                 350         


Lys Val Val Ser Gln Tyr His Glu Leu Val Val Gln Ala Arg Asp Asp 
        355                 360                 365             


Phe Lys Arg Glu Leu Asp Ser Ile Thr Pro Glu Val Leu Pro Gly Trp 
    370                 375                 380                 


Lys Gly Met Ser Val Ser Asp Leu Ala Asp Lys Leu Ser Thr Asp Asp 
385                 390                 395                 400 


Leu Asn Ser Leu Ile Ala His Ala His Arg Arg Ile Asp Gln Leu Asn 
                405                 410                 415     


Arg Glu Leu Ala Glu Gln Lys Ala Thr Glu Lys Gln His Ile Thr Leu 
            420                 425                 430         


Ala Leu Glu Lys Gln Lys Leu Glu Glu Lys Arg Ala Phe Asp Ser Ala 
        435                 440                 445             


Val Ala Lys Ala Leu Glu His His Arg Ser Glu Ile Gln Ala Glu Gln 
    450                 455                 460                 


Asp Arg Lys Ile Glu Glu Val Arg Asp Ala Met Glu Asn Glu Met Arg 
465                 470                 475                 480 


Thr Gln Leu Arg Arg Gln Ala Ala Ala His Thr Asp His Leu Arg Asp 
                485                 490                 495     


Val Leu Arg Val Gln Glu Gln Glu Leu Lys Ser Glu Phe Glu Gln Asn 
            500                 505                 510         


Leu Ser Glu Lys Leu Ser Glu Gln Glu Leu Gln Phe Arg Arg Leu Ser 
        515                 520                 525             


Gln Glu Gln Val Asp Asn Phe Thr Leu Asp Ile Asn Thr Ala Tyr Ala 
    530                 535                 540                 


Arg Leu Arg Gly Ile Glu Gln Ala Val Gln Ser His Ala Val Ala Glu 
545                 550                 555                 560 


Glu Glu Ala Arg Lys Ala His Gln Leu Trp Leu Ser Val Glu Ala Leu 
                565                 570                 575     


Lys Tyr Ser Met Lys Thr Ser Ser Ala Glu Thr Pro Thr Ile Pro Leu 
            580                 585                 590         


Gly Ser Ala Val Glu Ala Ile Lys Ala Asn Cys Ser Asp Asn Glu Phe 
        595                 600                 605             


Thr Gln Ala Leu Thr Ala Ala Ile Pro Pro Glu Ser Leu Thr Arg Gly 
    610                 615                 620                 


Val Tyr Ser Glu Glu Thr Leu Arg Ala Arg Phe Tyr Ala Val Gln Lys 
625                 630                 635                 640 


Leu Ala Arg Arg Val Ala Met Ile Asp Glu Thr Arg Asn Ser Leu Tyr 
                645                 650                 655     


Gln Tyr Phe Leu Ser Tyr Leu Gln Ser Leu Leu Leu Phe Pro Pro Gln 
            660                 665                 670         


Gln Leu Lys Pro Pro Pro Glu Leu Cys Pro Glu Asp Ile Asn Thr Phe 
        675                 680                 685             


Lys Leu Leu Ser Tyr Ala Ser Tyr Cys Ile Glu His Gly Asp Leu Glu 
    690                 695                 700                 


Leu Ala Ala Lys Phe Val Asn Gln Leu Lys Gly Glu Ser Arg Arg Val 
705                 710                 715                 720 


Ala Gln Asp Trp Leu Lys Glu Ala Arg Met Thr Leu Glu Thr Lys Gln 
                725                 730                 735     


Ile Val Glu Ile Leu Thr Ala Tyr Ala Ser Ala Val Gly Ile Gly Thr 
            740                 745                 750         


Thr Gln Val Gln Pro Glu 
        755             


<210>  2
<211>  65
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(65)
<223>  first 65 aa of human IMMT sequence

<400>  2

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Ala Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Gly Ser Ser Gly Leu Thr Thr Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala 
65  


<210>  3
<211>  187
<212>  PRT
<213>  Homo sapiens


<220>
<221>  SITE
<222>  (1)..(187)
<223>  first 187 aa of human IMMT sequence

<400>  3

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Ala Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Gly Ser Ser Gly Leu Thr Thr Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Glu Met Val Leu Gly Pro Ala Ala Tyr Asn 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Ser Ile Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Lys Glu Ser Lys Gln Pro Ala Ser Gln Leu 
        115                 120                 125             


Gln Lys Gln Lys Gly Asp Thr Pro Ala Ser Ala Thr Ala Pro Thr Glu 
    130                 135                 140                 


Ala Ala Gln Ile Ile Ser Ala Ala Gly Asp Thr Leu Ser Val Pro Ala 
145                 150                 155                 160 


Pro Ala Val Gln Pro Glu Glu Ser Leu Lys Thr Asp His Pro Glu Ile 
                165                 170                 175     


Gly Glu Gly Lys Pro Thr Pro Ala Leu Ser Glu 
            180                 185         


<210>  4
<211>  757
<212>  PRT
<213>  Mus musculus


<220>
<221>  SITE
<222>  (1)..(757)
<223>  Mouse IMMT (UniProtKB - Q8CAQ8)

<400>  4

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Leu Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Gly Met Val Leu Gly Ser Ala Pro Tyr Thr 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Pro Val Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Lys Asp Ser Lys Leu Pro Val Ala Gln Ser 
        115                 120                 125             


Gln Lys Thr Lys Gly Asp Thr Pro Ala Ser Ala Ala Ser Thr Gly Ala 
    130                 135                 140                 


Ala Gln Ile Ile Ser Ala Ala Gly Asp Thr Leu Ser Val Pro Ala Pro 
145                 150                 155                 160 


Ala Val Gln His Glu Asp Thr Ile Lys Thr Glu Cys Pro Asn Thr Asn 
                165                 170                 175     


Glu Gly Lys Ser Thr Ser Glu Thr Thr Glu Glu Ala Phe Ser Ser Ser 
            180                 185                 190         


Val Arg Glu Arg Pro Pro Glu Glu Val Ala Ala Arg Leu Ala Gln Gln 
        195                 200                 205             


Glu Lys Gln Glu Gln Val Glu Met Glu Ser Leu Ala Lys Ser Leu Glu 
    210                 215                 220                 


Asp Ala Leu Asn Arg Thr Ser Ser Val Thr Leu Gln Thr Ile Thr Ala 
225                 230                 235                 240 


Gln Asn Ala Ala Val Gln Ala Val Lys Ala His Ser Asn Ile Leu Lys 
                245                 250                 255     


Thr Ala Met Asp Asn Ser Glu Ile Ala Gly Glu Lys Lys Ser Ala Gln 
            260                 265                 270         


Trp Arg Thr Val Glu Gly Ala Leu Lys Glu Arg Arg Lys Ala Val Asp 
        275                 280                 285             


Glu Ala Ala Asp Ala Leu Leu Lys Ala Lys Glu Glu Leu Glu Lys Met 
    290                 295                 300                 


Lys Thr Ile Ile Glu Asp Ala Lys Lys Arg Glu Ile Ala Gly Ala Thr 
305                 310                 315                 320 


Pro His Ile Thr Ala Ala Glu Gly Arg Leu His Asn Met Ile Val Asp 
                325                 330                 335     


Leu Asp Asn Val Val Lys Lys Val Gln Ala Ala Gln Ser Glu Ala Lys 
            340                 345                 350         


Val Val Ser Gln Tyr His Glu Leu Val Val Gln Ala Arg Asp Asp Phe 
        355                 360                 365             


Arg Lys Glu Leu Asp Ser Ile Thr Pro Asp Ile Thr Pro Gly Trp Lys 
    370                 375                 380                 


Gly Met Ser Ile Ser Asp Leu Ala Gly Lys Leu Ser Thr Asp Asp Leu 
385                 390                 395                 400 


Asn Ser Leu Ile Ala His Ala His Arg Arg Ile Asp Gln Leu Asn Arg 
                405                 410                 415     


Glu Leu Ala Gln Gln Lys Ala Thr Glu Lys Gln His Ile Glu Leu Ala 
            420                 425                 430         


Leu Glu Lys His Lys Leu Glu Glu Lys Arg Thr Phe Asp Ser Ala Val 
        435                 440                 445             


Ala Lys Ala Leu Glu His His Arg Ser Glu Ile Gln Ala Glu Gln Asp 
    450                 455                 460                 


Arg Lys Val Glu Glu Val Arg Asp Ala Met Glu Asn Glu Met Arg Thr 
465                 470                 475                 480 


Gln Leu Arg Arg Gln Ala Ala Ala His Thr Asp His Leu Arg Asp Val 
                485                 490                 495     


Leu Lys Val Gln Glu Gln Glu Leu Lys Tyr Glu Phe Glu Gln Gly Leu 
            500                 505                 510         


Ser Glu Lys Leu Ser Glu Gln Glu Leu Glu Phe Arg Arg Arg Ser Gln 
        515                 520                 525             


Glu Gln Met Asp Ser Phe Thr Leu Asp Ile Asn Thr Ala Tyr Ala Arg 
    530                 535                 540                 


Leu Arg Gly Ile Glu Gln Ala Val Gln Ser His Ala Val Ala Glu Glu 
545                 550                 555                 560 


Glu Ala Arg Lys Ala His Gln Leu Trp Leu Ser Val Glu Ala Leu Lys 
                565                 570                 575     


Tyr Ser Met Lys Thr Ser Ser Ala Glu Met Pro Thr Ile Pro Leu Gly 
            580                 585                 590         


Ser Ala Val Glu Ala Ile Arg Val Asn Cys Ser Asp Asn Glu Phe Thr 
        595                 600                 605             


Gln Ala Leu Thr Ala Ala Ile Pro Pro Glu Ser Leu Thr Arg Gly Val 
    610                 615                 620                 


Tyr Ser Glu Glu Thr Leu Arg Ala Arg Phe Tyr Ala Val Gln Lys Leu 
625                 630                 635                 640 


Ala Arg Arg Val Ala Met Ile Asp Glu Thr Arg Asn Ser Leu Tyr Gln 
                645                 650                 655     


Tyr Phe Leu Ser Tyr Leu Gln Ser Leu Leu Leu Phe Pro Pro Lys Gln 
            660                 665                 670         


Leu Lys Pro Pro Ala Glu Leu Tyr Pro Glu Asp Ile Asn Thr Phe Lys 
        675                 680                 685             


Leu Leu Ser Tyr Ala Ser Tyr Cys Ile Glu His Gly Asp Leu Glu Leu 
    690                 695                 700                 


Ala Ala Lys Phe Val Asn Gln Leu Lys Gly Glu Ser Arg Arg Val Ala 
705                 710                 715                 720 


Gln Asp Trp Leu Lys Glu Ala Arg Met Thr Leu Glu Thr Lys Gln Ile 
                725                 730                 735     


Val Glu Ile Leu Thr Ala Tyr Ala Ser Ala Val Gly Ile Gly Thr Thr 
            740                 745                 750         


Gln Val Gln Gln Glu 
        755         


<210>  5
<211>  65
<212>  PRT
<213>  Mus musculus


<220>
<221>  SITE
<222>  (1)..(65)
<223>  first 65 aa of mouse IMMT sequence

<400>  5

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Leu Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala 
65  


<210>  6
<211>  187
<212>  PRT
<213>  Mus musculus


<220>
<221>  SITE
<222>  (1)..(187)
<223>  first 187 aa of mouse IMMT sequence

<400>  6

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Leu Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Gly Met Val Leu Gly Ser Ala Pro Tyr Thr 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Pro Val Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Lys Asp Ser Lys Leu Pro Val Ala Gln Ser 
        115                 120                 125             


Gln Lys Thr Lys Gly Asp Thr Pro Ala Ser Ala Ala Ser Thr Gly Ala 
    130                 135                 140                 


Ala Gln Ile Ile Ser Ala Ala Gly Asp Thr Leu Ser Val Pro Ala Pro 
145                 150                 155                 160 


Ala Val Gln His Glu Asp Thr Ile Lys Thr Glu Cys Pro Asn Thr Asn 
                165                 170                 175     


Glu Gly Lys Ser Thr Ser Glu Thr Thr Glu Glu 
            180                 185         


<210>  7
<211>  609
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(609)
<223>  Rat IMMT (UniProtKB - Q3KR86); would use aa 1-187; minimum aa 
       1-66)

<400>  7

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Val Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Gly Met Val Leu Gly Ser Ala Pro Tyr Thr 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Pro Ile Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Thr Asp Ser Glu Leu Pro Met Ala Gln Thr 
        115                 120                 125             


Gln Glu Thr Asn Gly Asp Thr Pro Ala Ser Ala Ala Gly Asp Pro Ala 
    130                 135                 140                 


Pro Glu Val Glu His Glu Asp Thr Ile Asn Thr Glu Cys Pro Asn Thr 
145                 150                 155                 160 


Asp Glu Gly Thr Ser Thr Phe Val Thr Ala Ala Leu Ala Lys Ser Leu 
                165                 170                 175     


Glu Asp Ala Leu Asn Gln Thr Ala Thr Val Thr Arg Gln Thr Ile Thr 
            180                 185                 190         


Ala Gln Asn Ala Ala Val Gln Ala Val Lys Ala His Ser Ser Thr Leu 
        195                 200                 205             


Lys Thr Ala Met Asp Asn Ser Glu Ile Ala Gly Glu Lys Lys Ser Ala 
    210                 215                 220                 


Gln Trp Arg Thr Val Glu Gly Ala Leu Lys Glu Arg Arg Lys Ala Val 
225                 230                 235                 240 


Asp Glu Ala Ala Asp Ala Leu Leu Lys Ala Lys Glu Glu Leu Glu Lys 
                245                 250                 255     


Met Lys Thr Ile Ile Glu Asp Ala Lys Lys Arg Glu Ile Ala Gly Ala 
            260                 265                 270         


Thr Pro Tyr Ile Thr Ala Ala Glu Glu Lys Leu His Ser Met Ile Val 
        275                 280                 285             


Asp Leu Asp Ser Val Val Lys Lys Val Gln Ala Ala Gln Ser Glu Ala 
    290                 295                 300                 


Lys Val Val Ser Gln Tyr His Glu Leu Val Val Gln Ala Arg Asp Asp 
305                 310                 315                 320 


Phe Arg Lys Glu Leu Asp Ser Ile Thr Pro Asp Ile Thr Pro Gly Trp 
                325                 330                 335     


Lys Gly Met Ser Ile Ser Asp Leu Ala Gly Thr Leu Ser Thr Asp Asp 
            340                 345                 350         


Leu Asn Ala Leu Ile Ala His Ala His Arg Arg Ile Asp Gln Leu Asn 
        355                 360                 365             


Arg Glu Leu Ala Gln Gln Lys Ala Thr Glu Lys Gln His Ile Glu Leu 
    370                 375                 380                 


Ala Leu Glu Arg Gln Lys Leu Glu Glu Lys Arg Ala Phe Asp Ser Ala 
385                 390                 395                 400 


Val Ala Lys Ala Leu Glu His His Arg Ser Glu Ile Gln Ala Glu Gln 
                405                 410                 415     


Asp Arg Lys Val Glu Glu Val Arg Asp Ala Met Glu Asn Glu Met Arg 
            420                 425                 430         


Thr Gln Leu Arg Arg Gln Ala Ala Ala His Thr Asp His Leu Arg Asp 
        435                 440                 445             


Val Leu Lys Val Gln Glu Gln Glu Leu Lys Phe Glu Phe Glu Gln Asp 
    450                 455                 460                 


Leu Ser Glu Lys Leu Ser Glu Gln Glu Leu Glu Phe His Arg Arg Ser 
465                 470                 475                 480 


Gln Glu Gln Met Asp Asn Phe Thr Leu Asp Ile Asn Thr Ala Tyr Ala 
                485                 490                 495     


Arg Leu Arg Gly Ile Glu Gln Ala Val Gln Ser His Ala Val Ala Glu 
            500                 505                 510         


Glu Glu Ala Arg Lys Ala His Gln Leu Trp Leu Ser Val Glu Ala Leu 
        515                 520                 525             


Lys Tyr Ser Met Lys Thr Ser Ser Ala Glu Met Pro Thr Ile Pro Leu 
    530                 535                 540                 


Gly Ser Ala Val Glu Ala Ile Arg Val Ser Cys Ser Asp Asn Glu Phe 
545                 550                 555                 560 


Thr Gln Ala Leu Thr Ala Ala Ile Pro Pro Glu Ser Leu Thr Arg Gly 
                565                 570                 575     


Val Tyr Ser Glu Glu Thr Leu Arg Ala Arg Phe Tyr Ala Val Gln Lys 
            580                 585                 590         


Leu Ala Gly Arg Val Ala Met Ile Asp Glu Thr Lys Asn Ser Leu Tyr 
        595                 600                 605             


Gln 
    


<210>  8
<211>  66
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(66)
<223>  first 66 aa of rat IMMT sequence

<400>  8

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Val Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys 
65      


<210>  9
<211>  187
<212>  PRT
<213>  Rattus norvegicus


<220>
<221>  SITE
<222>  (1)..(187)
<223>  first 187 aa of rat IMMT sequence

<400>  9

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Val Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Gly Met Val Leu Gly Ser Ala Pro Tyr Thr 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Pro Ile Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Thr Asp Ser Glu Leu Pro Met Ala Gln Thr 
        115                 120                 125             


Gln Glu Thr Asn Gly Asp Thr Pro Ala Ser Ala Ala Gly Asp Pro Ala 
    130                 135                 140                 


Pro Glu Val Glu His Glu Asp Thr Ile Asn Thr Glu Cys Pro Asn Thr 
145                 150                 155                 160 


Asp Glu Gly Thr Ser Thr Phe Val Thr Ala Ala Leu Ala Lys Ser Leu 
                165                 170                 175     


Glu Asp Ala Leu Asn Gln Thr Ala Thr Val Thr 
            180                 185         


<210>  10
<211>  314
<212>  PRT
<213>  L. maculans


<220>
<221>  SITE
<222>  (1)..(314)
<223>  Mac (L. maculans) sequence

<400>  10

Leu Ile Val Asp Gln Phe Glu Glu Val Leu Met Lys Thr Ser Gln Leu 
1               5                   10                  15      


Phe Pro Leu Pro Thr Ala Thr Gln Ser Ala Gln Pro Thr His Val Ala 
            20                  25                  30          


Pro Val Pro Thr Val Leu Pro Asp Thr Pro Ile Tyr Glu Thr Val Gly 
        35                  40                  45              


Asp Ser Gly Ser Lys Thr Leu Trp Val Val Phe Val Leu Met Leu Ile 
    50                  55                  60                  


Ala Ser Ala Ala Phe Thr Ala Leu Ser Trp Lys Ile Pro Val Asn Arg 
65                  70                  75                  80  


Arg Leu Tyr His Val Ile Thr Thr Ile Ile Thr Leu Thr Ala Ala Leu 
                85                  90                  95      


Ser Tyr Phe Ala Met Ala Thr Gly His Gly Val Ala Leu Asn Lys Ile 
            100                 105                 110         


Val Ile Arg Thr Gln His Asp His Val Pro Asp Thr Tyr Glu Thr Val 
        115                 120                 125             


Tyr Arg Gln Val Tyr Tyr Ala Arg Tyr Ile Asp Trp Ala Ile Thr Thr 
    130                 135                 140                 


Pro Leu Leu Leu Leu Asp Leu Gly Leu Leu Ala Gly Met Ser Gly Ala 
145                 150                 155                 160 


His Ile Phe Met Ala Ile Val Ala Asp Leu Ile Met Val Leu Thr Gly 
                165                 170                 175     


Leu Phe Ala Ala Phe Gly Ser Glu Gly Thr Pro Gln Lys Trp Gly Trp 
            180                 185                 190         


Tyr Thr Ile Ala Cys Ile Ala Tyr Ile Phe Val Val Trp His Leu Val 
        195                 200                 205             


Leu Asn Gly Gly Ala Asn Ala Arg Val Lys Gly Glu Lys Leu Arg Ser 
    210                 215                 220                 


Phe Phe Val Ala Ile Gly Ala Tyr Thr Leu Ile Leu Trp Thr Ala Tyr 
225                 230                 235                 240 


Pro Ile Val Trp Gly Leu Ala Asp Gly Ala Arg Lys Ile Gly Val Asp 
                245                 250                 255     


Gly Glu Ile Ile Ala Tyr Ala Val Leu Asp Val Leu Ala Lys Gly Val 
            260                 265                 270         


Phe Gly Ala Trp Leu Leu Val Thr His Ala Asn Leu Arg Glu Ser Asp 
        275                 280                 285             


Val Glu Leu Asn Gly Phe Trp Ala Asn Gly Leu Asn Arg Glu Gly Ala 
    290                 295                 300                 


Ile Arg Ile Gly Glu Asp Asp Gly Ala Arg 
305                 310                 


<210>  11
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Complete mtON fusion protein sequence

<400>  11

Met Leu Arg Ala Cys Gln Leu Ser Gly Val Thr Val Ala Ala Gln Ser 
1               5                   10                  15      


Cys Leu Cys Gly Lys Phe Val Leu Arg Pro Leu Arg Pro Cys Arg Arg 
            20                  25                  30          


Tyr Ser Thr Ser Ser Ser Ser Gly Leu Thr Ala Gly Lys Ile Ala Gly 
        35                  40                  45              


Ala Gly Leu Leu Phe Val Gly Gly Gly Ile Gly Gly Thr Ile Leu Tyr 
    50                  55                  60                  


Ala Lys Trp Asp Ser His Phe Arg Glu Ser Val Glu Lys Thr Ile Pro 
65                  70                  75                  80  


Tyr Ser Asp Lys Leu Phe Gly Met Val Leu Gly Ser Ala Pro Tyr Thr 
                85                  90                  95      


Val Pro Leu Pro Lys Lys Pro Val Gln Ser Gly Pro Leu Lys Ile Ser 
            100                 105                 110         


Ser Val Ser Glu Val Met Lys Asp Ser Lys Leu Pro Val Ala Gln Ser 
        115                 120                 125             


Gln Lys Thr Lys Gly Asp Thr Pro Ala Ser Ala Ala Ser Thr Gly Ala 
    130                 135                 140                 


Ala Gln Ile Ile Ser Ala Ala Gly Asp Thr Leu Ser Val Pro Ala Pro 
145                 150                 155                 160 


Ala Val Gln His Glu Asp Thr Ile Lys Thr Glu Cys Pro Asn Thr Asn 
                165                 170                 175     


Glu Gly Lys Ser Thr Ser Glu Thr Thr Glu Glu Pro Ala Gly Leu Ile 
            180                 185                 190         


Val Asp Gln Phe Glu Glu Val Leu Met Lys Thr Ser Gln Leu Phe Pro 
        195                 200                 205             


Leu Pro Thr Ala Thr Gln Ser Ala Gln Pro Thr His Val Ala Pro Val 
    210                 215                 220                 


Pro Thr Val Leu Pro Asp Thr Pro Ile Tyr Glu Thr Val Gly Asp Ser 
225                 230                 235                 240 


Gly Ser Lys Thr Leu Trp Val Val Phe Val Leu Met Leu Ile Ala Ser 
                245                 250                 255     


Ala Ala Phe Thr Ala Leu Ser Trp Lys Ile Pro Val Asn Arg Arg Leu 
            260                 265                 270         


Tyr His Val Ile Thr Thr Ile Ile Thr Leu Thr Ala Ala Leu Ser Tyr 
        275                 280                 285             


Phe Ala Met Ala Thr Gly His Gly Val Ala Leu Asn Lys Ile Val Ile 
    290                 295                 300                 


Arg Thr Gln His Asp His Val Pro Asp Thr Tyr Glu Thr Val Tyr Arg 
305                 310                 315                 320 


Gln Val Tyr Tyr Ala Arg Tyr Ile Asp Trp Ala Ile Thr Thr Pro Leu 
                325                 330                 335     


Leu Leu Leu Asp Leu Gly Leu Leu Ala Gly Met Ser Gly Ala His Ile 
            340                 345                 350         


Phe Met Ala Ile Val Ala Asp Leu Ile Met Val Leu Thr Gly Leu Phe 
        355                 360                 365             


Ala Ala Phe Gly Ser Glu Gly Thr Pro Gln Lys Trp Gly Trp Tyr Thr 
    370                 375                 380                 


Ile Ala Cys Ile Ala Tyr Ile Phe Val Val Trp His Leu Val Leu Asn 
385                 390                 395                 400 


Gly Gly Ala Asn Ala Arg Val Lys Gly Glu Lys Leu Arg Ser Phe Phe 
                405                 410                 415     


Val Ala Ile Gly Ala Tyr Thr Leu Ile Leu Trp Thr Ala Tyr Pro Ile 
            420                 425                 430         


Val Trp Gly Leu Ala Asp Gly Ala Arg Lys Ile Gly Val Asp Gly Glu 
        435                 440                 445             


Ile Ile Ala Tyr Ala Val Leu Asp Val Leu Ala Lys Gly Val Phe Gly 
    450                 455                 460                 


Ala Trp Leu Leu Val Thr His Ala Asn Leu Arg Glu Ser Asp Val Glu 
465                 470                 475                 480 


Leu Asn Gly Phe Trp Ala Asn Gly Leu Asn Arg Glu Gly Ala Ile Arg 
                485                 490                 495     


Ile Gly Glu Asp Asp Gly Ala Arg 
            500                 


<210>  12
<211>  1512
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: mtON nucleotide sequence

<400>  12
atgctgcggg cctgtcagtt atccggcgtg accgtcgcgg cccagagttg tctgtgtggg       60

aagttcgtcc tccgtccact acgaccatgc cgtagatact ccacttcaag cagttctggg      120

ttgactgctg gtaagattgc tggagctggc cttttgttcg ttggtggagg gattggtggc      180

actatcctgt atgccaaatg ggattcccat ttccgggaaa gtgtagagaa aaccatccct      240

tactcagaca aactctttgg gatggttctt ggttctgcgc cttatactgt tccattacca      300

aagaaaccgg ttcagtctgg tccactaaaa atctccagtg tatcagaggt gatgaaagac      360

tccaaactgc ctgtggcaca gagccagaaa acgaagggag acactccagc ttcagcagca      420

tctacagggg cagctcaaat tatttctgca gcaggtgata cactttcggt cccggcccct      480

gcagttcagc atgaggacac tataaaaacc gaatgtccca acactaatga aggaaaatct      540

acgtctgaaa ccacagagga acctgcaggc ttgatcgtgg accagttcga ggaggtgctg      600

atgaagacca gccagctgtt cccactgcca accgctaccc agagcgccca gccaacccac      660

gtggcccccg tgccaaccgt gctgcccgac acccccatct acgagaccgt gggcgacagc      720

ggcagcaaga ccctgtgggt ggtgttcgtg ctgatgctga tcgccagcgc cgccttcacc      780

gccctgagct ggaagatccc cgtgaacagg aggctgtacc acgtgatcac caccatcatc      840

accctgaccg ccgccctgag ctacttcgct atggctaccg gccacggagt ggccctgaac      900

aagatcgtga tcaggaccca gcacgaccac gtgcccgaca cctacgagac cgtgtaccga      960

caggtgtact acgccaggta catcgactgg gctatcacca ccccactgct gctgctggac     1020

ctgggactgc tggctggaat gagcggagcc cacatcttca tggccatcgt ggctgacctg     1080

atcatggtgc tgaccggcct gttcgctgct ttcggcagcg agggaacccc acagaagtgg     1140

ggatggtaca ccatcgcctg catcgcctac atcttcgtgg tgtggcacct ggtgctgaac     1200

ggcggcgcca acgccagggt gaagggcgag aagctgagga gcttcttcgt ggccatcgga     1260

gcttacaccc tgatcctgtg gaccgcttac ccaatcgtgt ggggactggc tgacggagct     1320

aggaagatcg gagtggacgg agagatcatc gcttacgctg tgctggacgt gctggctaag     1380

ggagtgttcg gagcttggct gctggtgacc cacgccaacc tgagggagag cgacgtggag     1440

ctgaacggct tctgggccaa cggcctgaac agggagggcg ccatcaggat cggcgaggac     1500

gacggcgccc ga                                                         1512


<210>  13
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for Mac-GFP

<400>  13
acacctgcag gcttgatcgt ggaccagttc ga                                     32


<210>  14
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for Mac-GFP

<400>  14
cacagcggcc gcttacttgt acagctcgtc ca                                     32


<210>  15
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for Immt1

<400>  15
acaaccggta aaaatgctgc gggcctgtca gtt                                    33


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for Immt1

<400>  16
caccctgcag gttcctctgt ggtttcagac g                                      31


<210>  17
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for promoter Peft-3 (also
       known as Peef-1A.1)

<400>  17
aacaaagctt gcacctttgg tctttta                                           27


<210>  18
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for promoter Peft-3 (also
       known as Peef-1A.1)

<400>  18
acatctagag agcaaagtgt ttccca                                            26


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for promoter Pmyo-3

<400>  19
acagctagct gtgtgtgatt gct                                               23


<210>  20
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for promoter Pmyo-3

<400>  20
acaaccggtg cggcaattct agatgg                                            26


<210>  21
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for red fluorescent 
       protein mCherry

<400>  21
ctcgcccagc ttcttgtggc tcacgccgcc gtctcatcga ttcgctcaac ttatcctcgt       60

agccctcgga ctcctcgtag catggtctca aagggtgaag a                          101


<210>  22
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for red fluorescent 
       protein mCherry

<400>  22
ttttaaaaaa cggtgacgca agacaatcaa ttgttctact tatacaattc atccatgcc        59


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DNA target of crRNA

<400>  23
ctaataagtt gagcgaatcg                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for uaDf5 mitochondrial 
       DNA

<400>  24
ttggtgttac aggggcaaca                                                   20


<210>  25
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for uaDf5 mitochondrial 
       DNA

<400>  25
cttctacagt gcattgacct agtc                                              24


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Forward amplification primer for uaDf5 DNA

<400>  26
ccatccgtgc tagaagacaa                                                   20


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Reverse amplification primer for uaDf5 DNA

<400>  27
cttctacagt gcattgacct agtc                                              24


