                         SEQUENCE LISTING

<110>  Zoetis Services LLC
       Strietzel, Catherine J.
       Lightle, Sandra Ann Marie
       Campos, Henry Luis
       Zhu, Yaqi
       Fici, Gregory
       Bally, Alexander
       Shanbhogue, Prajna	
 
<120>  BOVINE ANTIBODY VARIANTS

<130>  ZP000367A

<160>  113    

<170>  PatentIn version 3.5

<210>  1
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Wildtype (NCBI ID number 1S82409)

<400>  1

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  2
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1b Wildtype (NCBI ID number X16701)

<400>  2

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  3
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1c Wildtype (NCBI ID number DQ452014.1)

<400>  3

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Ser Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Asp Pro Arg Cys Lys Arg Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asn Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  4
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1d Wildtype (NCBI ID number X62916.1)

<400>  4

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  5
<211>  327
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Wildtype (NCBI ID number X16702.1)

<400>  5

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Gly Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Ser Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys 
            100                 105                 110         


Val Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
        115                 120                 125             


Leu Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val 
    130                 135                 140                 


Gly His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val 
145                 150                 155                 160 


Glu Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
                165                 170                 175     


Thr Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr 
            180                 185                 190         


Gly Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala 
        195                 200                 205             


Ser Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro 
    210                 215                 220                 


Gln Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr 
225                 230                 235                 240 


Val Ser Leu Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp 
                245                 250                 255     


Val Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg 
            260                 265                 270         


Thr Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser 
        275                 280                 285             


Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr 
    290                 295                 300                 


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Met Gln Lys Ser 
305                 310                 315                 320 


Thr Ser Lys Ser Ala Gly Lys 
                325         


<210>  6
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2b Wildtype (NCBI ID number S82407)

<400>  6

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ala Ser Ser Cys Gly 
1               5                   10                  15      


Asp Thr Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
                245                 250                 255     


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Leu His Asn His Tyr Met Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  7
<211>  352
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Wildtype

<400>  7

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ala Ser Ser Cys Gly 
1               5                   10                  15      


Asp Thr Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Arg Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Ser Ser Glu Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile 
            100                 105                 110         


Pro Pro Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro 
        115                 120                 125             


Cys Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe 
    130                 135                 140                 


Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val 
                165                 170                 175     


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala 
        195                 200                 205             


Leu Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys 
    210                 215                 220                 


Lys Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser 
225                 230                 235                 240 


Arg Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro 
                245                 250                 255     


Pro Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile 
            260                 265                 270         


Thr Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly 
        275                 280                 285             


Gln Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp 
    290                 295                 300                 


Ala Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser 
305                 310                 315                 320 


Ser Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala 
                325                 330                 335     


Leu Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
            340                 345                 350         


<210>  8
<211>  352
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3b Wildtype

<400>  8

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ala Ser Arg Cys Gly 
1               5                   10                  15      


Asp Thr Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Thr Ser Glu Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile 
            100                 105                 110         


Pro Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro 
        115                 120                 125             


Cys Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe 
    130                 135                 140                 


Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val 
                165                 170                 175     


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala 
        195                 200                 205             


Leu Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys 
    210                 215                 220                 


Lys Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser 
225                 230                 235                 240 


Arg Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro 
                245                 250                 255     


Pro Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile 
            260                 265                 270         


Thr Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly 
        275                 280                 285             


Gln Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp 
    290                 295                 300                 


Ala Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser 
305                 310                 315                 320 


Ser Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala 
                325                 330                 335     


Leu Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
            340                 345                 350         


<210>  9
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Wildtype from Dairy Cow

<400>  9

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Leu His Asn His Tyr Met Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  10
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Having DP1 Mutation

<400>  10

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Glu Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  11
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Having DP2 Mutation

<400>  11

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Glu Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  12
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Having DP1 and DP2 Mutations

<400>  12

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Glu Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Glu Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  13
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Wildtype Fragment Physical Positions 99-329

<400>  13

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Leu 
1               5                   10                  15      


Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Pro Ala 
            100                 105                 110         


Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  14
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Fragment Having SAP Mutation

<400>  14

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Leu 
1               5                   10                  15      


Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Ser Ala 
            100                 105                 110         


Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  15
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Fragment Having SAS Mutation

<400>  15

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Leu 
1               5                   10                  15      


Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Ser Ala 
            100                 105                 110         


Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  16
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Fragment Having Winter Mutations

<400>  16

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Ala 
1               5                   10                  15      


Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Pro Ala 
            100                 105                 110         


Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  17
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Fragment Having Winter and SAS Mutations

<400>  17

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Ala 
1               5                   10                  15      


Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Ser Ala 
            100                 105                 110         


Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  18
<211>  231
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG1a Fragment Having Winter and SS Mutations

<400>  18

Asp Pro Arg Cys Lys Thr Thr Cys Asp Cys Cys Pro Pro Pro Glu Ala 
1               5                   10                  15      


Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp Asp Val 
    50                  55                  60                  


Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp Trp Thr 
                85                  90                  95      


Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys Ser Thr 
    130                 135                 140                 


Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr Ile Ala 
145                 150                 155                 160 


Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys Tyr Gly 
                165                 170                 175     


Thr Thr Pro Pro Gln Leu Asp Ala Asp Gly Ser Tyr Phe Leu Tyr Ser 
            180                 185                 190         


Arg Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr Tyr Thr 
        195                 200                 205             


Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
    210                 215                 220                 


Thr Ser Lys Ser Ala Gly Lys 
225                 230     


<210>  19
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutation L432A

<400>  19

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Ala His Asn His Tyr Met Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  20
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutation N434A

<400>  20

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Leu His Ala His Tyr Met Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  21
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutation M437A

<400>  21

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Leu His Asn His Tyr Ala Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  22
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutations L432A and M437A

<400>  22

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Ala His Asn His Tyr Ala Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  23
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutations N434A and M437A

<400>  23

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Leu His Ala His Tyr Ala Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  24
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutations L432A, N434A, and M437A

<400>  24

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Ala His Ala His Tyr Ala Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  25
<211>  326
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG2a Having Mutations L432A and N434A

<400>  25

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Thr Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Arg Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Ser Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Gly Val Ser Ser Asp Cys Ser Lys Pro Asn Asn Gln His Cys Val 
            100                 105                 110         


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
        115                 120                 125             


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
    130                 135                 140                 


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
145                 150                 155                 160 


Val His Thr Ala Arg Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
                165                 170                 175     


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
            180                 185                 190         


Gly Lys Glu Phe Lys Cys Lys Val Asn Ile Lys Gly Leu Ser Ala Ser 
        195                 200                 205             


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
    210                 215                 220                 


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Val 
225                 230                 235                 240 


Ser Val Thr Cys Met Val Ile Gly Phe Tyr Pro Glu Asp Val Asp Val 
                245                 250                 255     


Glu Trp Gln Arg Asp Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
            260                 265                 270         


Thr Pro Pro Gln Leu Asp Ala Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Arg Val Asp Arg Asn Ser Trp Gln Arg Gly Asp Thr Tyr Thr Cys 
    290                 295                 300                 


Val Val Met His Glu Ala Ala His Ala His Tyr Met Gln Lys Ser Thr 
305                 310                 315                 320 


Ser Lys Ser Ala Gly Lys 
                325     


<210>  26
<211>  254
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Wildtype Fragment Physical Positions 99-352

<400>  26

Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro Pro 
1               5                   10                  15      


Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro Cys Gln 
            20                  25                  30          


Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile Phe 
        35                  40                  45              


Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val 
    50                  55                  60                  


Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln Phe 
65                  70                  75                  80  


Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys Pro 
                85                  90                  95      


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg 
            100                 105                 110         


Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys Val 
        115                 120                 125             


Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr 
    130                 135                 140                 


Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Arg 
145                 150                 155                 160 


Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr Gly 
                165                 170                 175     


Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
            180                 185                 190         


Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala Asp 
        195                 200                 205             


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser Ser Trp 
    210                 215                 220                 


Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu Arg 
225                 230                 235                 240 


Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
                245                 250                 


<210>  27
<211>  254
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Fragment Having SAP Mutation

<400>  27

Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro Pro 
1               5                   10                  15      


Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro Cys Gln 
            20                  25                  30          


Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile Phe 
        35                  40                  45              


Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val 
    50                  55                  60                  


Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln Phe 
65                  70                  75                  80  


Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys Pro 
                85                  90                  95      


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg 
            100                 105                 110         


Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys Val 
        115                 120                 125             


Asn Asn Lys Gly Leu Ser Ala Pro Ile Val Arg Thr Ile Ser Arg Thr 
    130                 135                 140                 


Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Arg 
145                 150                 155                 160 


Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr Gly 
                165                 170                 175     


Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
            180                 185                 190         


Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala Asp 
        195                 200                 205             


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser Ser Trp 
    210                 215                 220                 


Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu Arg 
225                 230                 235                 240 


Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
                245                 250                 


<210>  28
<211>  254
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Fragment Having SAS Mutation

<400>  28

Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro Pro 
1               5                   10                  15      


Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro Cys Gln 
            20                  25                  30          


Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile Phe 
        35                  40                  45              


Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val 
    50                  55                  60                  


Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln Phe 
65                  70                  75                  80  


Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys Pro 
                85                  90                  95      


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg 
            100                 105                 110         


Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys Val 
        115                 120                 125             


Asn Asn Lys Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser Arg Thr 
    130                 135                 140                 


Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Arg 
145                 150                 155                 160 


Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr Gly 
                165                 170                 175     


Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
            180                 185                 190         


Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala Asp 
        195                 200                 205             


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser Ser Trp 
    210                 215                 220                 


Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu Arg 
225                 230                 235                 240 


Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
                245                 250                 


<210>  29
<211>  254
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Fragment Having Winter Mutation

<400>  29

Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro Pro 
1               5                   10                  15      


Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro Cys Gln 
            20                  25                  30          


Cys Ser Lys Cys Pro Glu Ala Ala Gly Ala Leu Ser Val Phe Ile Phe 
        35                  40                  45              


Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val 
    50                  55                  60                  


Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln Phe 
65                  70                  75                  80  


Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys Pro 
                85                  90                  95      


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg 
            100                 105                 110         


Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys Val 
        115                 120                 125             


Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr 
    130                 135                 140                 


Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Arg 
145                 150                 155                 160 


Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr Gly 
                165                 170                 175     


Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
            180                 185                 190         


Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala Asp 
        195                 200                 205             


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser Ser Trp 
    210                 215                 220                 


Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu Arg 
225                 230                 235                 240 


Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
                245                 250                 


<210>  30
<211>  254
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Fragment Having Winter and SAS Mutations

<400>  30

Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro Pro 
1               5                   10                  15      


Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro Cys Gln 
            20                  25                  30          


Cys Ser Lys Cys Pro Glu Ala Ala Gly Ala Leu Ser Val Phe Ile Phe 
        35                  40                  45              


Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val 
    50                  55                  60                  


Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln Phe 
65                  70                  75                  80  


Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys Pro 
                85                  90                  95      


Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg 
            100                 105                 110         


Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys Val 
        115                 120                 125             


Asn Asn Lys Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser Arg Thr 
    130                 135                 140                 


Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Arg 
145                 150                 155                 160 


Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr Gly 
                165                 170                 175     


Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln Pro 
            180                 185                 190         


Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala Asp 
        195                 200                 205             


Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser Ser Trp 
    210                 215                 220                 


Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu Arg 
225                 230                 235                 240 


Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
                245                 250                 


<210>  31
<211>  352
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  IgG3a Having Mutation R433H

<400>  31

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ala Ser Ser Cys Gly 
1               5                   10                  15      


Asp Thr Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Arg Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Ser Ser Glu Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile 
            100                 105                 110         


Pro Pro Gly Lys Pro Thr Thr Pro Lys Ser Glu Val Glu Lys Thr Pro 
        115                 120                 125             


Cys Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe 
    130                 135                 140                 


Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val 
                165                 170                 175     


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala 
        195                 200                 205             


Leu Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys 
    210                 215                 220                 


Lys Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser 
225                 230                 235                 240 


Arg Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro 
                245                 250                 255     


Pro Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile 
            260                 265                 270         


Thr Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly 
        275                 280                 285             


Gln Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp 
    290                 295                 300                 


Ala Asp Gly Ser Tyr Phe Leu Tyr Ser Lys Leu Arg Val Asn Lys Ser 
305                 310                 315                 320 


Ser Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala 
                325                 330                 335     


Leu His Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
            340                 345                 350         


<210>  32
<211>  330
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  human IgG1

<400>  32

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 
                         

<210>  33
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b Wildtype.

<400>  33

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  34
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b Wildtype.

<400>  34
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gagctgcctg gaggaccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgcc agctcccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  35
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having Winter mutation.

<400>  35

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Ala Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  36
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having Winter mutation.

<400>  36
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gaggccgccg gagccccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgcc agctcccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  37
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having WinSS mutation.

<400>  37

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Ala Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Pro Ser Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  38
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having WinSS mutation.

<400>  38
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gaggccgccg gagccccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgcc atcctccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  39
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having WinSAS mutation.

<400>  39

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Ala Ala Gly Ala Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Ser Ala Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  40
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having WinSAS mutation.

<400>  40
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gaggccgccg gagccccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgtc cgcttccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  41
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having SAS mutation.

<400>  41

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Ser Ala Ser Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  42
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having SAS mutation.

<400>  42
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gagctgcctg gaggaccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgtc cgcttccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  43
<211>  377
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having SAP mutation.

<400>  43

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
145                 150                 155                 160 


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
                165                 170                 175     


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
            180                 185                 190         


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
        195                 200                 205             


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
    210                 215                 220                 


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
225                 230                 235                 240 


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
                245                 250                 255     


Ser Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
            260                 265                 270         


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
        275                 280                 285             


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
    290                 295                 300                 


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
305                 310                 315                 320 


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
            340                 345                 350         


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
        355                 360                 365             


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
    370                 375         


<210>  44
<211>  1131
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having SAP mutation.

<400>  44
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcagaggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctgc gcgccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagatt ctgacgatcc tacctgcaag ccctcccctt gtgactgctg tccacctcca      480

gagctgcctg gaggaccatc cgtgttcatc tttcccccta agccaaagga cacactgacc      540

atcagcggca caccagaggt gacctgcgtg gtggtggatg tgggccacga cgatccagag      600

gtgaagttct cttggtttgt ggacgatgtg gaggtgaaca ccgccaccac aaagcccagg      660

gaggagcagt tcaatagcac atatagagtg gtgtctgctc tgcgcatcca gcaccaggat      720

tggaccggcg gcaaggagtt taagtgcaag gtgcataacg agggcctgtc cgctcccatc      780

gtgagaacaa tctcccgcac caagggacca gccagggagc ctcaggtgta cgtgctggct      840

ccaccccagg aggagctgtc taagtccaca gtgtccctga cctgtatggt gacctccttc      900

taccccgact atatcgctgt ggagtggcag aggaatggac agcctgagag cgaggataag      960

tatggcacca cacctccaca gctggacgct gattccagct actttctgta tagcaagctg     1020

agggtggacc ggaactcttg gcaggagggc gatacataca cctgcgtggt catgcatgag     1080

gccctgcaca atcattatac ccagaagagc acatctaagt ccgctggcaa g              1131


<210>  45
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having D216E mutation.

<400>  45

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Glu Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Asp Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  46
<211>  987
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having D216E mutation.

<400>  46
gcctctacca cagctccaaa ggtgtatccc ctgtcttcct gctgtggcga taagagctct       60

tccaccgtga cactgggctg tctggtgagc tcttacatgc ccgagcctgt gaccgtgaca      120

tggaactctg gcgccctgaa gtccggcgtg cacaccttcc cagctgtgct gcagtccagc      180

ggcctgtaca gcctgtcttc catggtgaca gtgcccggct ctacctccgg ccagaccttt      240

acatgcaatg tggcccatcc tgctagctct acaaaggtgg acaaggccgt ggagccaacc      300

tgcaagccaa gcccctgtga ctgctgtcca cctccagagc tgccaggagg accttccgtg      360

ttcatctttc cccctaagcc aaaggacacc ctgacaatct ccggcacccc agaggtgaca      420

tgcgtggtgg tggatgtggg ccacgacgat cctgaggtga agttctcttg gtttgtggac      480

gatgtggagg tgaacacagc caccacaaag ccacgggagg agcagttcaa tagcacctac      540

agggtggtgt ctgctctgcg gatccagcac caggattgga ccggcggcaa ggagtttaag      600

tgcaaggtgc ataacgaggg cctgcctgct ccaatcgtga gaaccatctc ccgcacaaag      660

ggacctgcca gggagccaca ggtgtatgtg ctggctccac cccaggagga gctgagcaag      720

tctaccgtgt ccctgacatg tatggtgacc agcttctatc ccgactacat cgccgtggag      780

tggcagagga atggccagcc tgagagcgag gataagtacg gcaccacacc tccacagctg      840

gacgctgatt ccagctattt tctgtactct aagctgaggg tggaccggaa ctcctggcag      900

gagggcgata cctatacatg cgtggtcatg catgaggccc tgcacaatca ttacacacag      960

aagtccacca gcaagtctgc tggcaag                                          987


<210>  47
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having D270E mutation.

<400>  47

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Asp Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Glu Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  48
<211>  987
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having D270E mutation.

<400>  48
gcctctacca cagctccaaa ggtgtatccc ctgtcttcct gctgtggcga taagagctct       60

tccaccgtga cactgggctg tctggtgagc tcttacatgc ccgagcctgt gaccgtgaca      120

tggaactctg gcgccctgaa gtccggcgtg cacaccttcc cagctgtgct gcagtccagc      180

ggcctgtaca gcctgtcttc catggtgaca gtgcccggct ctacctccgg ccagaccttt      240

acatgcaatg tggcccatcc tgctagctct acaaaggtgg acaaggccgt ggatccaacc      300

tgcaagccaa gcccctgtga ctgctgtcca cctccagagc tgccaggagg accttccgtg      360

ttcatctttc cccctaagcc aaaggacacc ctgacaatct ccggcacccc agaggtgaca      420

tgcgtggtgg tggatgtggg ccacgacgag cctgaggtga agttctcttg gtttgtggac      480

gatgtggagg tgaacacagc caccacaaag ccacgggagg agcagttcaa tagcacctac      540

agggtggtgt ctgctctgcg gatccagcac caggattgga ccggcggcaa ggagtttaag      600

tgcaaggtgc ataacgaggg cctgcctgct ccaatcgtga gaaccatctc ccgcacaaag      660

ggacctgcca gggagccaca ggtgtatgtg ctggctccac cccaggagga gctgagcaag      720

tctaccgtgt ccctgacatg tatggtgacc agcttctatc ccgactacat cgccgtggag      780

tggcagagga atggccagcc tgagagcgag gataagtacg gcaccacacc tccacagctg      840

gacgctgatt ccagctattt tctgtactct aagctgaggg tggaccggaa ctcctggcag      900

gagggcgata cctatacatg cgtggtcatg catgaggccc tgcacaatca ttacacacag      960

aagtccacca gcaagtctgc tggcaag                                          987


<210>  49
<211>  329
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG1b having D216E and D270E 
       mutations.

<400>  49

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ser Ser Cys Cys Gly 
1               5                   10                  15      


Asp Lys Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Gly Ser Thr Ser Gly Gln Thr Phe 
65                  70                  75                  80  


Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Ala 
                85                  90                  95      


Val Glu Pro Thr Cys Lys Pro Ser Pro Cys Asp Cys Cys Pro Pro Pro 
            100                 105                 110         


Glu Leu Pro Gly Gly Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Gly His Asp Glu Pro Glu Val Lys Phe Ser Trp Phe Val Asp 
145                 150                 155                 160 


Asp Val Glu Val Asn Thr Ala Thr Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Ala Leu Arg Ile Gln His Gln Asp 
            180                 185                 190         


Trp Thr Gly Gly Lys Glu Phe Lys Cys Lys Val His Asn Glu Gly Leu 
        195                 200                 205             


Pro Ala Pro Ile Val Arg Thr Ile Ser Arg Thr Lys Gly Pro Ala Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Val Leu Ala Pro Pro Gln Glu Glu Leu Ser Lys 
225                 230                 235                 240 


Ser Thr Val Ser Leu Thr Cys Met Val Thr Ser Phe Tyr Pro Asp Tyr 
                245                 250                 255     


Ile Ala Val Glu Trp Gln Arg Asn Gly Gln Pro Glu Ser Glu Asp Lys 
            260                 265                 270         


Tyr Gly Thr Thr Pro Pro Gln Leu Asp Ala Asp Ser Ser Tyr Phe Leu 
        275                 280                 285             


Tyr Ser Lys Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Thr 
    290                 295                 300                 


Tyr Thr Cys Val Val Met His Glu Ala Leu His Asn His Tyr Thr Gln 
305                 310                 315                 320 


Lys Ser Thr Ser Lys Ser Ala Gly Lys 
                325                 


<210>  50
<211>  987
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG1b having D216E and D270E 
       mutations.

<400>  50
gcctctacca cagctccaaa ggtgtatccc ctgtcttcct gctgtggcga taagagctct       60

tccaccgtga cactgggctg tctggtgagc tcttacatgc ccgagcctgt gaccgtgaca      120

tggaactctg gcgccctgaa gtccggcgtg cacaccttcc cagctgtgct gcagtccagc      180

ggcctgtaca gcctgtcttc catggtgaca gtgcccggct ctacctccgg ccagaccttt      240

acatgcaatg tggcccatcc tgctagctct acaaaggtgg acaaggccgt ggagccaacc      300

tgcaagccaa gcccctgtga ctgctgtcca cctccagagc tgccaggagg accttccgtg      360

ttcatctttc cccctaagcc aaaggacacc ctgacaatct ccggcacccc agaggtgaca      420

tgcgtggtgg tggatgtggg ccacgacgag cctgaggtga agttctcttg gtttgtggac      480

gatgtggagg tgaacacagc caccacaaag ccacgggagg agcagttcaa tagcacctac      540

agggtggtgt ctgctctgcg gatccagcac caggattgga ccggcggcaa ggagtttaag      600

tgcaaggtgc ataacgaggg cctgcctgct ccaatcgtga gaaccatctc ccgcacaaag      660

ggacctgcca gggagccaca ggtgtatgtg ctggctccac cccaggagga gctgagcaag      720

tctaccgtgt ccctgacatg tatggtgacc agcttctatc ccgactacat cgccgtggag      780

tggcagagga atggccagcc tgagagcgag gataagtacg gcaccacacc tccacagctg      840

gacgctgatt ccagctattt tctgtactct aagctgaggg tggaccggaa ctcctggcag      900

gagggcgata cctatacatg cgtggtcatg catgaggccc tgcacaatca ttacacacag      960

aagtccacca gcaagtctgc tggcaag                                          987


<210>  51
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b Wildtype.

<400>  51

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Leu His Asn His Tyr Met Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  52
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b Wildtype.

<400>  52
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctctgcac     1080

aatcattata tgcagaagag cacatctaag tccgctggca ag                        1122


<210>  53
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having L432A mutation.

<400>  53

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Ala His Asn His Tyr Met Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  54
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having L432A mutation.

<400>  54
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctgcccac     1080

aatcattata tgcagaagag cacatctaag tccgctggca ag                        1122


<210>  55
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having N434A mutation.

<400>  55

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Leu His Ala His Tyr Met Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  56
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having N434A mutation.

<400>  56
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctctgcac     1080

gcccattata tgcagaagag cacatctaag tccgctggca ag                        1122


<210>  57
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having M437A mutation.

<400>  57
Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Leu His Asn His Tyr Ala Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  58
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having M437A mutation.

<400>  58
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctctgcac     1080

aatcattatg cccagaagag cacatctaag tccgctggca ag                        1122


<210>  59
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having L432A and N434A 
       mutations.

<400>  59

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Ala His Ala His Tyr Met Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  60
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having L432A and N434A 
       mutations.

<400>  60
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctgcccac     1080

gcccattata tgcagaagag cacatctaag tccgctggca ag                        1122


<210>  61
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having L432A and M437A 
       mutations.

<400>  61

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Ala His Asn His Tyr Ala Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  62
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having L432A and M437A 
       mutations.

<400>  62
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctgcccac     1080

aatcattatg cccagaagag cacatctaag tccgctggca ag                        1122


<210>  63
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having N434A and M437A 
       mutations.

<400>  63

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Leu His Ala His Tyr Ala Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  64
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having N434A and M437A 
       mutations.

<400>  64
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctctgcac     1080

gcccattatg cccagaagag cacatctaag tccgctggca ag                        1122


<210>  65
<211>  374
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG2b having L432A, N434A and M437A 
       mutations.

<400>  65

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Gly Val Ser Ile Asp Cys Ser Lys Cys His Asn Gln Pro Cys Val 
145                 150                 155                 160 


Arg Glu Pro Ser Val Phe Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu 
                165                 170                 175     


Met Ile Thr Gly Thr Pro Glu Val Thr Cys Val Val Val Asn Val Gly 
            180                 185                 190         


His Asp Asn Pro Glu Val Gln Phe Ser Trp Phe Val Asp Asp Val Glu 
        195                 200                 205             


Val His Thr Ala Arg Ser Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
    210                 215                 220                 


Tyr Arg Val Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Thr Gly 
225                 230                 235                 240 


Gly Lys Glu Phe Lys Cys Lys Val Asn Asn Lys Gly Leu Ser Ala Pro 
                245                 250                 255     


Ile Val Arg Ile Ile Ser Arg Ser Lys Gly Pro Ala Arg Glu Pro Gln 
            260                 265                 270         


Val Tyr Val Leu Asp Pro Pro Lys Glu Glu Leu Ser Lys Ser Thr Leu 
        275                 280                 285             


Ser Val Thr Cys Met Val Thr Gly Phe Tyr Pro Glu Asp Val Ala Val 
    290                 295                 300                 


Glu Trp Gln Arg Asn Arg Gln Thr Glu Ser Glu Asp Lys Tyr Arg Thr 
305                 310                 315                 320 


Thr Pro Pro Gln Leu Asp Thr Asp Arg Ser Tyr Phe Leu Tyr Ser Lys 
                325                 330                 335     


Leu Arg Val Asp Arg Asn Ser Trp Gln Glu Gly Asp Ala Tyr Thr Cys 
            340                 345                 350         


Val Val Met His Glu Ala Ala His Ala His Tyr Ala Gln Lys Ser Thr 
        355                 360                 365             


Ser Lys Ser Ala Gly Lys 
    370                 


<210>  66
<211>  1122
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG2b having L432A, N434A and M437A 
       mutations.

<400>  66
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcccgg cagcaccggc       60

gatatgcacg tggctcagcc tgctgtggtg ctggcctcca gccggggcgt ggcttctttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggatccc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg atagcacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgta aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaacggc acccagatct acgtgatcga ccccgagcct      420

tgcccagaca gcgatggcgt gtctatcgat tgctccaagt gtcacaatca gccatgcgtg      480

agggagccct ccgtgttcat ctttccccct aagcctaagg acaccctgat gatcacaggc      540

accccagagg tgacatgcgt ggtggtgaac gtgggccacg ataatcccga ggtgcagttc      600

tcttggtttg tggacgatgt ggaggtgcat accgccaggt ccaagccacg ggaggagcag      660

ttcaactcca catacagggt ggtgtccgcc ctgcccatcc agcatcagga ctggaccggc      720

ggcaaggagt ttaagtgcaa ggtgaacaat aagggcctga gcgcccctat cgtgagaatc      780

atctcccgca gcaagggacc agctcgggag cctcaggtgt atgtgctgga tccacccaag      840

gaggagctgt ctaagtccac actgtctgtg acctgtatgg tgacaggctt ctacccagag      900

gacgtggccg tggagtggca gaggaatcgg cagaccgagt ccgaggataa gtatagaacc      960

acacctccac agctggacac agatcgcagc tactttctgt attctaagct gagagtggac     1020

cgcaactctt ggcaggaggg cgatgcctac acctgcgtgg tcatgcatga ggctgcccac     1080

gcccattatg cccagaagag cacatctaag tccgctggca ag                        1122


<210>  67
<211>  399
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b Wildtype.

<400>  67

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro 
145                 150                 155                 160 


Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro Cys 
                165                 170                 175     


Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln 
    210                 215                 220                 


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu 
                245                 250                 255     


Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser Arg 
        275                 280                 285             


Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro 
    290                 295                 300                 


Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr 
305                 310                 315                 320 


Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 
                325                 330                 335     


Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala 
            340                 345                 350         


Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser Ser 
        355                 360                 365             


Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu 
    370                 375                 380                 


Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
385                 390                 395                 


<210>  68
<211>  1197
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b Wildtype.

<400>  68
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcaggggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagaca gcgatacagc taggcggccc gtgcctacca cacccaagac cacaatcccc      480

cctggcaagc ctaccacaca ggagtctgag gtggagaaga ccccctgcca gtgttccaag      540

tgcccagagc cactgggagg actgagcgtg ttcatctttc cacccaagcc aaaggacaca      600

ctgaccatct ccggcacacc agaggtgacc tgcgtggtgg tggatgtggg ccaggacgat      660

ccagaggtgc agttcagctg gtttgtggac gatgtggagg tgcacacagc caggaccaag      720

ccccgggagg agcagttcaa ctccacctac agagtggtgt ccgccctgcg catccagcat      780

caggattggc tgcagggcaa ggagtttaag tgcaaggtga acaataaggg cctgcctgcc      840

ccaatcgtga gaacaatctc tcgcaccaag ggccaggcca gagagcctca ggtgtatgtg      900

ctggctcctc cacgcgagga gctgtctaag tccacactgt ctctgacctg tctgatcaca      960

ggcttctacc cagaggagat cgacgtggag tggcagagga atggccagcc cgagtccgag     1020

gataagtatc acaccacagc ccctcagctg gacgctgatg gcagctactt tctgtattct     1080

agactgcgcg tgaacaagtc cagctggcag gagggcgacc attacacctg tgccgtgatg     1140

cacgaggctc tgaggaatca ttataaggag aagagcatct ctcggtcccc tggcaag        1197


<210>  69
<211>  399
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b having Winter mutation.

<400>  69

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro 
145                 150                 155                 160 


Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro Cys 
                165                 170                 175     


Gln Cys Ser Lys Cys Pro Glu Ala Ala Gly Ala Leu Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln 
    210                 215                 220                 


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu 
                245                 250                 255     


Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser Arg 
        275                 280                 285             


Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro 
    290                 295                 300                 


Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr 
305                 310                 315                 320 


Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 
                325                 330                 335     


Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala 
            340                 345                 350         


Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser Ser 
        355                 360                 365             


Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu 
    370                 375                 380                 


Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
385                 390                 395                 


<210>  70
<211>  1197
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b having Winter mutation.

<400>  70
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcaggggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagaca gcgatacagc taggcggccc gtgcctacca cacccaagac cacaatcccc      480

cctggcaagc ctaccacaca ggagtctgag gtggagaaga ccccctgcca gtgttccaag      540

tgcccagagg ccgccggagc cctgagcgtg ttcatctttc cacccaagcc aaaggacaca      600

ctgaccatct ccggcacacc agaggtgacc tgcgtggtgg tggatgtggg ccaggacgat      660

ccagaggtgc agttcagctg gtttgtggac gatgtggagg tgcacacagc caggaccaag      720

ccccgggagg agcagttcaa ctccacctac agagtggtgt ccgccctgcg catccagcat      780

caggattggc tgcagggcaa ggagtttaag tgcaaggtga acaataaggg cctgcctgcc      840

ccaatcgtga gaacaatctc tcgcaccaag ggccaggcca gagagcctca ggtgtatgtg      900

ctggctcctc cacgcgagga gctgtctaag tccacactgt ctctgacctg tctgatcaca      960

ggcttctacc cagaggagat cgacgtggag tggcagagga atggccagcc cgagtccgag     1020

gataagtatc acaccacagc ccctcagctg gacgctgatg gcagctactt tctgtattct     1080

agactgcgcg tgaacaagtc cagctggcag gagggcgacc attacacctg tgccgtgatg     1140

cacgaggctc tgaggaatca ttataaggag aagagcatct ctcggtcccc tggcaag        1197


<210>  71
<211>  399
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b having WinSAS mutation.

<400>  71

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro 
145                 150                 155                 160 


Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro Cys 
                165                 170                 175     


Gln Cys Ser Lys Cys Pro Glu Ala Ala Gly Ala Leu Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln 
    210                 215                 220                 


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu 
                245                 250                 255     


Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser Arg 
        275                 280                 285             


Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro 
    290                 295                 300                 


Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr 
305                 310                 315                 320 


Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 
                325                 330                 335     


Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala 
            340                 345                 350         


Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser Ser 
        355                 360                 365             


Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu 
    370                 375                 380                 


Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
385                 390                 395                 


<210>  72
<211>  1197
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b having WinSAS mutation.

<400>  72
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcaggggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagaca gcgatacagc taggcggccc gtgcctacca cacccaagac cacaatcccc      480

cctggcaagc ctaccacaca ggagtctgag gtggagaaga ccccctgcca gtgttccaag      540

tgcccagagg ccgccggagc cctgagcgtg ttcatctttc cacccaagcc aaaggacaca      600

ctgaccatct ccggcacacc agaggtgacc tgcgtggtgg tggatgtggg ccaggacgat      660

ccagaggtgc agttcagctg gtttgtggac gatgtggagg tgcacacagc caggaccaag      720

ccccgggagg agcagttcaa ctccacctac agagtggtgt ccgccctgcg catccagcat      780

caggattggc tgcagggcaa ggagtttaag tgcaaggtga acaataaggg cctgtccgcc      840

tccatcgtga gaacaatctc tcgcaccaag ggccaggcca gagagcctca ggtgtatgtg      900

ctggctcctc cacgcgagga gctgtctaag tccacactgt ctctgacctg tctgatcaca      960

ggcttctacc cagaggagat cgacgtggag tggcagagga atggccagcc cgagtccgag     1020

gataagtatc acaccacagc ccctcagctg gacgctgatg gcagctactt tctgtattct     1080

agactgcgcg tgaacaagtc cagctggcag gagggcgacc attacacctg tgccgtgatg     1140

cacgaggctc tgaggaatca ttataaggag aagagcatct ctcggtcccc tggcaag        1197


<210>  73
<211>  399
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b having SAS mutation.

<400>  73

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro 
145                 150                 155                 160 


Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro Cys 
                165                 170                 175     


Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln 
    210                 215                 220                 


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu 
                245                 250                 255     


Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser Arg 
        275                 280                 285             


Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro 
    290                 295                 300                 


Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr 
305                 310                 315                 320 


Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 
                325                 330                 335     


Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala 
            340                 345                 350         


Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser Ser 
        355                 360                 365             


Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu 
    370                 375                 380                 


Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
385                 390                 395                 


<210>  74
<211>  1197
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b having SAS mutation.

<400>  74
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcaggggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagaca gcgatacagc taggcggccc gtgcctacca cacccaagac cacaatcccc      480

cctggcaagc ctaccacaca ggagtctgag gtggagaaga ccccctgcca gtgttccaag      540

tgcccagagc cactgggagg actgagcgtg ttcatctttc cacccaagcc aaaggacaca      600

ctgaccatct ccggcacacc agaggtgacc tgcgtggtgg tggatgtggg ccaggacgat      660

ccagaggtgc agttcagctg gtttgtggac gatgtggagg tgcacacagc caggaccaag      720

ccccgggagg agcagttcaa ctccacctac agagtggtgt ccgccctgcg catccagcat      780

caggattggc tgcagggcaa ggagtttaag tgcaaggtga acaataaggg cctgtccgcc      840

tccatcgtga gaacaatctc tcgcaccaag ggccaggcca gagagcctca ggtgtatgtg      900

ctggctcctc cacgcgagga gctgtctaag tccacactgt ctctgacctg tctgatcaca      960

ggcttctacc cagaggagat cgacgtggag tggcagagga atggccagcc cgagtccgag     1020

gataagtatc acaccacagc ccctcagctg gacgctgatg gcagctactt tctgtattct     1080

agactgcgcg tgaacaagtc cagctggcag gagggcgacc attacacctg tgccgtgatg     1140

cacgaggctc tgaggaatca ttataaggag aagagcatct ctcggtcccc tggcaag        1197


<210>  75
<211>  399
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b having SAP mutation.

<400>  75

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp Met His Val Ala Gln Pro Ala Val Val Leu Ala 
            20                  25                  30          


Ser Ser Arg Gly Val Ala Ser Phe Val Cys Glu Tyr Gly Ser Ser Gly 
        35                  40                  45              


Asn Ala Ala Glu Val Arg Val Thr Val Leu Arg Gln Ala Gly Ser Gln 
    50                  55                  60                  


Met Thr Glu Val Cys Ala Ala Thr Tyr Thr Val Glu Asp Glu Leu Ala 
65                  70                  75                  80  


Phe Leu Asp Asp Ser Thr Cys Thr Gly Thr Ser Ser Gly Asn Lys Val 
                85                  90                  95      


Asn Leu Thr Ile Gln Gly Leu Arg Ala Met Asp Thr Gly Leu Tyr Ile 
            100                 105                 110         


Cys Lys Val Glu Leu Met Tyr Pro Pro Pro Tyr Tyr Val Gly Met Gly 
        115                 120                 125             


Asn Gly Thr Gln Ile Tyr Val Ile Asp Pro Glu Pro Cys Pro Asp Ser 
    130                 135                 140                 


Asp Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile Pro 
145                 150                 155                 160 


Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro Cys 
                165                 170                 175     


Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe Ile 
            180                 185                 190         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro Glu 
        195                 200                 205             


Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val Gln 
    210                 215                 220                 


Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr Lys 
225                 230                 235                 240 


Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala Leu 
                245                 250                 255     


Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys Lys 
            260                 265                 270         


Val Asn Asn Lys Gly Leu Ser Ala Pro Ile Val Arg Thr Ile Ser Arg 
        275                 280                 285             


Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro Pro 
    290                 295                 300                 


Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile Thr 
305                 310                 315                 320 


Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly Gln 
                325                 330                 335     


Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp Ala 
            340                 345                 350         


Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser Ser 
        355                 360                 365             


Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala Leu 
    370                 375                 380                 


Arg Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
385                 390                 395                 


<210>  76
<211>  1197
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b having SAP mutation.

<400>  76
atggagaccg acacactgct gctgtgggtg ctgctgctgt gggtgcctgg ctctaccggc       60

gatatgcacg tggctcagcc agctgtggtg ctggcctcca gcaggggcgt ggcttccttc      120

gtgtgcgagt acggctcttc cggaaacgct gctgaggtga gggtgaccgt gctgaggcag      180

gctggaagcc agatgacaga ggtgtgcgcc gctacctata cagtggagga cgagctggct      240

tttctggacg attctacctg tacaggcacc agctctggca acaaggtcaa tctgaccatc      300

cagggcctga gagccatgga tacaggcctg tacatctgca aggtggagct gatgtatccc      360

cctccatact atgtgggcat gggcaatggc acccagatct acgtgatcga ccccgagcct      420

tgtccagaca gcgatacagc taggcggccc gtgcctacca cacccaagac cacaatcccc      480

cctggcaagc ctaccacaca ggagtctgag gtggagaaga ccccctgcca gtgttccaag      540

tgcccagagc cactgggagg actgagcgtg ttcatctttc cacccaagcc aaaggacaca      600

ctgaccatct ccggcacacc agaggtgacc tgcgtggtgg tggatgtggg ccaggacgat      660

ccagaggtgc agttcagctg gtttgtggac gatgtggagg tgcacacagc caggaccaag      720

ccccgggagg agcagttcaa ctccacctac agagtggtgt ccgccctgcg catccagcat      780

caggattggc tgcagggcaa ggagtttaag tgcaaggtga acaataaggg cctgtccgcc      840

ccaatcgtga gaacaatctc tcgcaccaag ggccaggcca gagagcctca ggtgtatgtg      900

ctggctcctc cacgcgagga gctgtctaag tccacactgt ctctgacctg tctgatcaca      960

ggcttctacc cagaggagat cgacgtggag tggcagagga atggccagcc cgagtccgag     1020

gataagtatc acaccacagc ccctcagctg gacgctgatg gcagctactt tctgtattct     1080

agactgcgcg tgaacaagtc cagctggcag gagggcgacc attacacctg tgccgtgatg     1140

cacgaggctc tgaggaatca ttataaggag aagagcatct ctcggtcccc tggcaag        1197


<210>  77
<211>  352
<212>  PRT
<213>  Bos taurus


<220>
<221>  MISC_FEATURE
<223>  The amino acid sequence of IgG3b having R433H mutation.

<400>  77

Ala Ser Thr Thr Ala Pro Lys Val Tyr Pro Leu Ala Ser Arg Cys Gly 
1               5                   10                  15      


Asp Thr Ser Ser Ser Thr Val Thr Leu Gly Cys Leu Val Ser Ser Tyr 
            20                  25                  30          


Met Pro Glu Pro Val Thr Val Thr Trp Asn Ser Gly Ala Leu Lys Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Met Val Thr Val Pro Ala Ser Thr Ser Glu Thr Gln Thr 
65                  70                  75                  80  


Phe Thr Cys Asn Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys 
                85                  90                  95      


Ala Val Thr Ala Arg Arg Pro Val Pro Thr Thr Pro Lys Thr Thr Ile 
            100                 105                 110         


Pro Pro Gly Lys Pro Thr Thr Gln Glu Ser Glu Val Glu Lys Thr Pro 
        115                 120                 125             


Cys Gln Cys Ser Lys Cys Pro Glu Pro Leu Gly Gly Leu Ser Val Phe 
    130                 135                 140                 


Ile Phe Pro Pro Lys Pro Lys Asp Thr Leu Thr Ile Ser Gly Thr Pro 
145                 150                 155                 160 


Glu Val Thr Cys Val Val Val Asp Val Gly Gln Asp Asp Pro Glu Val 
                165                 170                 175     


Gln Phe Ser Trp Phe Val Asp Asp Val Glu Val His Thr Ala Arg Thr 
            180                 185                 190         


Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Ala 
        195                 200                 205             


Leu Arg Ile Gln His Gln Asp Trp Leu Gln Gly Lys Glu Phe Lys Cys 
    210                 215                 220                 


Lys Val Asn Asn Lys Gly Leu Pro Ala Pro Ile Val Arg Thr Ile Ser 
225                 230                 235                 240 


Arg Thr Lys Gly Gln Ala Arg Glu Pro Gln Val Tyr Val Leu Ala Pro 
                245                 250                 255     


Pro Arg Glu Glu Leu Ser Lys Ser Thr Leu Ser Leu Thr Cys Leu Ile 
            260                 265                 270         


Thr Gly Phe Tyr Pro Glu Glu Ile Asp Val Glu Trp Gln Arg Asn Gly 
        275                 280                 285             


Gln Pro Glu Ser Glu Asp Lys Tyr His Thr Thr Ala Pro Gln Leu Asp 
    290                 295                 300                 


Ala Asp Gly Ser Tyr Phe Leu Tyr Ser Arg Leu Arg Val Asn Lys Ser 
305                 310                 315                 320 


Ser Trp Gln Glu Gly Asp His Tyr Thr Cys Ala Val Met His Glu Ala 
                325                 330                 335     


Leu His Asn His Tyr Lys Glu Lys Ser Ile Ser Arg Ser Pro Gly Lys 
            340                 345                 350         


<210>  78
<211>  1056
<212>  DNA
<213>  Bos taurus


<220>
<221>  misc_feature
<223>  The nucleic acid sequence of IgG3b having R433H mutation.

<400>  78
gcctccacca cagctccaaa ggtgtatccc ctggccagca ggtgcggcga tacctcttcc       60

agcaccgtga cactgggctg tctggtgtct tcctacatgc ccgagcctgt gaccgtgaca      120

tggaactccg gcgccctgaa gagcggagtg cacaccttcc cagctgtgct gcagagctct      180

ggcctgtact ctctgtccag catggtgacc gtgcccgctt ccacaagcga gacccagacc      240

ttcacctgca atgtggccca tcctgcttct tccaccaagg tggataaggc tgtgacagct      300

aggcggccag tgcccaccac acccaagacc acaatccccc ctggcaagcc taccacacag      360

gagtccgagg tggagaagac cccctgccag tgtagcaagt gccctgagcc actgggagga      420

ctgtccgtgt tcatctttcc acccaagcca aaggacacac tgaccatctc tggcacaccc      480

gaggtgacct gcgtggtggt ggatgtgggc caggacgatc cagaggtgca gttctcctgg      540

tttgtggacg atgtggaggt gcacacagcc aggaccaagc cccgggagga gcagttcaac      600

agcacctata gggtggtgtc tgctctgcgg atccagcatc aggactggct gcagggcaag      660

gagtttaagt gcaaggtgaa caataagggc ctgcccgccc ctatcgtgag aacaatctcc      720

cgcaccaagg gacaggccag ggagcctcag gtgtacgtgc tggctcctcc acgggaggag      780

ctgtctaagt ccacactgag cctgacctgt ctgatcacag gcttctatcc agaggagatc      840

gacgtggagt ggcagagaaa tggccagcct gagtctgagg ataagtacca caccacagcc      900

ccacagctgg acgctgatgg ctcttatttt ctgtactcca gactgcgcgt gaacaagagc      960

tcttggcagg agggcgatca ttatacctgt gccgtgatgc acgaggctct gcacaatcat     1020

tacaaggaga agagcatctc tcgctcccct ggcaag                               1056


<210>  79
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG1bWin 
       (L234A_P235A_G237A): LPGG to AAGA.

<400>  79

Cys Asp Cys Cys Pro Pro Pro Glu Ala Ala Gly Ala Pro Ser Val Phe 
1               5                   10                  15      


Ile Phe Pro Pro 
            20  


<210>  80
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1bWin 
       (L234A_P235A_G237A): LPGG to AAGA

<400>  80
tgtgactgct gtccacctcc agaggccgcc ggagccccat ccgtgttcat ctttccccct       60


<210>  81
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flanking amino acid sequence of bIgG1bWinSS (A330S_P331S): PAP to
       PSS.

<400>  81

Lys Val His Asn Glu Gly Leu Pro Ser Ser Ile Val Arg Thr Ile Ser 
1               5                   10                  15      


Arg Thr Lys 
            


<210>  82
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1bWinSS (A330S_P331S): 
       PAP to PSS.

<400>  82
aaggtgcata acgagggcct gccatcctcc atcgtgagaa caatctcccg caccaag          57


<210>  83
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG1bSAS (P329S_P331S): PAP 
       to SAS.

<400>  83

Lys Val His Asn Glu Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser 
1               5                   10                  15      


Arg Thr Lys 
            


<210>  84
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1bSAS (P329S_P331S): 
       PAP to SAS.

<400>  84
aaggtgcata acgagggcct gtccgcttcc atcgtgagaa caatctcccg caccaag          57


<210>  85
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG1bSAP (P329S): PAP to 
       SAP.

<400>  85

Lys Val His Asn Glu Gly Leu Ser Ala Pro Ile Val Arg Thr Ile Ser 
1               5                   10                  15      


Arg Thr Lys 
            


<210>  86
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1bSAP (P329S): PAP to 
       SAP.

<400>  86
aaggtgcata acgagggcct gtccgctccc atcgtgagaa caatctcccg caccaag          57


<210>  87
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG1b_DP1 (D216E): DP1 to 
       EP1.

<400>  87

Thr Lys Val Asp Lys Ala Val Glu Pro Thr Cys Lys Pro Ser Pro Cys 
1               5                   10                  15      


Asp Cys Cys 
            


<210>  88
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1b_DP1 (D216E): DP1 to 
       EP1.

<400>  88
acaaaggtgg acaaggccgt ggagccaacc tgcaagccaa gcccctgtga ctgctgt          57


<210>  89
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG1b_DP2 (D270E): DP2 to 
       EP2.

<400>  89

Val Val Val Asp Val Gly His Asp Glu Pro Glu Val Lys Phe Ser Trp 
1               5                   10                  15      


Phe 
    


<210>  90
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG1b_DP2 (D270E): DP2 to 
       EP2.

<400>  90
gtggtggtgg atgtgggcca cgacgagcct gaggtgaagt tctcttggtt t                51


<210>  91
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_L432A: LHNHYM to 
       AHNHYM.

<400>  91

Val Met His Glu Ala Ala His Asn His Tyr Met Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  92
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_L432A: LHNHYM to 
       AHNHYM.

<400>  92
gtcatgcatg aggctgccca caatcattat atgcagaaga gcacatctaa g                51


<210>  93
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_N434A: LHNHYM to 
       LHAHYM.

<400>  93
Val Met His Glu Ala Leu His Ala His Tyr Met Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 


<210>  94
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_N434A: LHNHYM to 
       LHAHYM.

<400>  94
gtcatgcatg aggctctgca cgcccattat atgcagaaga gcacatctaa g                51


<210>  95
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_M437A: LHNHYM to 
       LHNHYA.

<400>  95

Val Met His Glu Ala Leu His Asn His Tyr Ala Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  96
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_M437A: LHNHYM to 
       LHNHYA.

<400>  96
gtcatgcatg aggctctgca caatcattat gcccagaaga gcacatctaa g                51


<210>  97
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_L432A_N434A: LHNHYM to
       AHNHYM; LHNHYM to LHAHYM.

<400>  97

Val Met His Glu Ala Ala His Ala His Tyr Met Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  98
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_L432A_N434A: LHNHYM 
       to AHNHYM; LHNHYM to LHAHYM.

<400>  98
gtcatgcatg aggctgccca cgcccattat atgcagaaga gcacatctaa g                51


<210>  99
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_L432A_M437A: LHNHYM to
       AHNHYM; LHNHYM to LHNHYA.

<400>  99

Val Met His Glu Ala Ala His Asn His Tyr Ala Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  100
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_L432A_M437A: LHNHYM 
       to AHNHYM; LHNHYM to LHNHYA.

<400>  100
gtcatgcatg aggctgccca caatcattat gcccagaaga gcacatctaa g                51


<210>  101
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_N434A_M437A: LHNHYM to
       LHAHYM; LHNHYM to LHNHYA.

<400>  101

Val Met His Glu Ala Leu His Ala His Tyr Ala Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  102
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_N434A_M437A: LHNHYM 
       to LHAHYM; LHNHYM to LHNHYA.

<400>  102
gtcatgcatg aggctctgca cgcccattat gcccagaaga gcacatctaa g                51


<210>  103
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG2b_L432A_N434A_M437A: 
       LHNHYM to AHNHYM; LHNHYM to LHAHYM; LHNHYM to LHNHYA.

<400>  103

Val Met His Glu Ala Ala His Ala His Tyr Ala Gln Lys Ser Thr Ser 
1               5                   10                  15      


Lys 
    


<210>  104
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG2b_L432A_N434A_M437A: 
       LHNHYM to AHNHYM; LHNHYM to LHAHYM; LHNHYM to LHNHYA.

<400>  104
gtcatgcatg aggctgccca cgcccattat gcccagaaga gcacatctaa g                51


<210>  105
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG3bWin 
       (P234A_L235A_G237A): PLGG to AAGA.

<400>  105

Gln Cys Ser Lys Cys Pro Glu Ala Ala Gly Ala Leu Ser Val Phe Ile 
1               5                   10                  15      


Phe Pro Pro 
            


<210>  106
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG3bWin 
       (P234A_L235A_G237A): PLGG to AAGA.

<400>  106
cagtgttcca agtgcccaga ggccgccgga gccctgagcg tgttcatctt tccaccc          57


<210>  107
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG3bSAS (P329S_P331S): PAP 
       to SAS.

<400>  107

Lys Val Asn Asn Lys Gly Leu Ser Ala Ser Ile Val Arg Thr Ile Ser 
1               5                   10                  15      


Arg Thr Lys 
            


<210>  108
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG3bSAS (P329S_P331S): 
       PAP to SAS.

<400>  108
aaggtgaaca ataagggcct gtccgcctcc atcgtgagaa caatctctcg caccaag          57


<210>  109
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG3bSAP (P329S): PAP to 
       SAP.

<400>  109

Lys Val Asn Asn Lys Gly Leu Ser Ala Pro Ile Val Arg Thr Ile Ser 
1               5                   10                  15      


Arg Thr Lys 
            


<210>  110
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG3bSAP (P329S): PAP to 
       SAP.

<400>  110
aaggtgaaca ataagggcct gtccgcccca atcgtgagaa caatctctcg caccaag          57


<210>  111
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The flanking amino acid sequence of bIgG3b_R433H: ALRNH to ALHNH.

<400>  111

Ala Val Met His Glu Ala Leu His Asn His Tyr Lys Glu Lys Ser Ile 
1               5                   10                  15      


Ser Arg 
        


<210>  112
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  The flanking nucleic acid sequence of bIgG3b_R433H: ALRNH to 
       ALHNH.

<400>  112
gccgtgatgc acgaggctct gcacaatcat tacaaggaga agagcatctc tcgc             54


<210>  113
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  The amino acid sequence of Biotin Acceptor Peptide (BAP).

<400>  113

Ala Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15      



