                         SEQUENCE LISTING

<110>  Greffex, Inc.
 
<120>  Design of Optimized Universal Influenza Vaccines, Their Designs 
       and Uses

<130>  543346.13

<150>  US 63/115,459
<151>  2020-11-18

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  343
<212>  PRT
<213>  Influenza A virus

<400>  1

Met Asn Thr Gln Ile Leu Ala Phe Ala Leu Leu Gly Ile Ala Val Thr 
1               5                   10                  15      


Asn Ala Asp Lys Ile Cys Leu Gly His His Ala Val Ser Asn Gly Thr 
            20                  25                  30          


Lys Val Asn Thr Leu Thr Glu Lys Gly Val Glu Val Val Asn Ala Thr 
        35                  40                  45              


Glu Thr Val Glu Ser Thr Ser Leu Lys Asp Ile Cys Gly Gly Gly Gly 
    50                  55                  60                  


Cys Glu Gly Glu Cys Phe Tyr Ser Gly Gly Thr Ile Asn Ser Lys Leu 
65                  70                  75                  80  


Pro Phe Gln Asn Ile Asp Ser Arg Ala Val Gly Lys Cys Pro Arg Tyr 
                85                  90                  95      


Val Lys Gln Lys Ser Leu Leu Leu Ala Thr Gly Met Lys Asn Val Pro 
            100                 105                 110         


Glu Val Ala Lys Arg Lys Arg Gln Ala Arg Gly Leu Phe Gly Ala Ile 
        115                 120                 125             


Ala Gly Phe Ile Glu Asn Gly Trp Glu Gly Leu Ile Asp Gly Trp Tyr 
    130                 135                 140                 


Gly Phe Arg His Gln Asn Ala Gln Gly Glu Gly Thr Ala Ala Asp Tyr 
145                 150                 155                 160 


Lys Ser Thr Gln Ala Ala Ile Asp Gln Ile Thr Gly Lys Leu Asn Arg 
                165                 170                 175     


Leu Ile Glu Lys Thr Asn Gln Gln Phe Glu Leu Ile Asp Asn Glu Phe 
            180                 185                 190         


Ser Glu Val Glu Gln Gln Ile Gly Asn Val Ile Asn Trp Thr Arg Asp 
        195                 200                 205             


Ser Ile Thr Glu Ile Trp Ser Tyr Asn Ala Glu Leu Leu Val Ala Met 
    210                 215                 220                 


Glu Asn Gln His Thr Ile Asp Leu Ala Asp Ser Glu Met Asn Lys Leu 
225                 230                 235                 240 


Tyr Glu Arg Val Arg Arg Gln Leu Arg Glu Asn Ala Glu Glu Asp Gly 
                245                 250                 255     


Thr Gly Cys Phe Glu Ile Phe His Lys Cys Asp Asp Asn Cys Met Glu 
            260                 265                 270         


Ser Ile Arg Asn Asn Thr Tyr Asp His Thr Gln Tyr Arg Glu Glu Ala 
        275                 280                 285             


Leu Gln Asn Arg Ile Gln Ile Asp Pro Val Lys Leu Ser Ser Gly Tyr 
    290                 295                 300                 


Lys Asp Val Ile Leu Trp Phe Ser Phe Gly Ala Ser Cys Phe Val Leu 
305                 310                 315                 320 


Leu Ala Ile Val Met Gly Leu Val Phe Ile Cys Val Lys Asn Gly Asn 
                325                 330                 335     


Met Arg Cys Thr Ile Cys Ile 
            340             


<210>  2
<211>  355
<212>  PRT
<213>  Influenza A virus

<400>  2

Met Leu Ser Ile Ile Val Leu Ser Leu Ile Leu Ala Glu Thr Ser Ala 
1               5                   10                  15      


Gln Lys Ile Ser Asn Asn Asp Thr Gly Asn Pro Val Ile Cys Leu Gly 
            20                  25                  30          


His His Ala Val Pro Asn Gly Thr Ile Val Lys Thr Leu Thr Asn Asp 
        35                  40                  45              


Gln Ile Glu Val Val Ser Ala Gln Glu Leu Val Glu Ser Ser Asn Ile 
    50                  55                  60                  


Asp Glu Leu Cys Gly Gly Gly Gly Cys Thr Ser Glu Cys Ile Thr Asp 
65                  70                  75                  80  


Lys Gly Ser Ile Ser Ser Asp Lys Pro Phe Gln Asn Val Ser Arg Ile 
                85                  90                  95      


Ala Ile Gly Asp Cys Pro Lys Tyr Val Lys Gln Ser Ser Leu Lys Leu 
            100                 105                 110         


Ala Thr Gly Met Arg Asn Ile Pro Glu Lys Gln Thr Arg Gly Leu Phe 
        115                 120                 125             


Gly Ala Ile Ala Gly Phe Ile Glu Asn Gly Trp Gln Gly Leu Ile Asp 
    130                 135                 140                 


Gly Trp Tyr Gly Phe Arg His Gln Asn Ala Glu Gly Thr Gly Thr Ala 
145                 150                 155                 160 


Ala Asp Leu Lys Ser Thr Gln Ala Ala Ile Asp Gln Ile Asn Gly Lys 
                165                 170                 175     


Leu Asn Arg Leu Ile Glu Lys Thr Asn Glu Lys Tyr His Gln Ile Glu 
            180                 185                 190         


Lys Glu Phe Glu Gln Val Glu Gly Arg Ile Gln Asp Leu Glu Lys Tyr 
        195                 200                 205             


Val Glu Asp Thr Lys Ile Asp Leu Trp Ser Tyr Asn Ala Glu Leu Leu 
    210                 215                 220                 


Val Ala Leu Glu Asn Gln His Thr Ile Asp Val Thr Asp Ser Glu Met 
225                 230                 235                 240 


Asn Lys Leu Phe Glu Arg Val Arg Arg Gln Leu Arg Glu Asn Ala Glu 
                245                 250                 255     


Asp Gln Gly Asn Gly Cys Phe Glu Ile Phe His Lys Cys Asp Asn Asn 
            260                 265                 270         


Cys Ile Glu Ser Ile Arg Asn Gly Thr Tyr Asp His Asn Ile Tyr Arg 
        275                 280                 285             


Asp Glu Ala Ile Asn Asn Arg Phe Gln Ile Lys Gly Val Glu Leu Thr 
    290                 295                 300                 


Gln Gly Tyr Lys Asp Val Ile Leu Trp Ile Ser Phe Ser Ile Ser Cys 
305                 310                 315                 320 


Phe Leu Leu Val Ala Leu Leu Leu Gly Phe Ile Leu Trp Ala Cys Gln 
                325                 330                 335     


Asn Gly Asn Ile Arg Cys Gln Ile Cys Ile Val His Leu Lys Thr Pro 
            340                 345                 350         


Leu Phe Leu 
        355 


<210>  3
<211>  347
<212>  PRT
<213>  Influenza A virus

<400>  3

Met Met Met Ala Leu Val Ile Leu Leu Leu Leu Phe Thr Thr Val Lys 
1               5                   10                  15      


Ser Asp Gln Ile Cys Ile Gly Tyr His Ala Asn Asn Ser Thr Glu Lys 
            20                  25                  30          


Val Asp Thr Ile Leu Glu Lys Asn Val Thr Val Thr His Ser Val Asn 
        35                  40                  45              


Leu Leu Glu Lys Thr His Asn Gly Lys Leu Cys Gly Gly Gly Gly Cys 
    50                  55                  60                  


Asn Thr Thr Cys Gln Thr Pro Glu Gly Ala Ile Asn Thr Ser Leu Pro 
65                  70                  75                  80  


Phe His Asn Val His Pro Leu Thr Ile Gly Glu Cys Pro Lys Tyr Val 
                85                  90                  95      


Lys Ser Glu Lys Leu Arg Leu Ala Thr Gly Leu Arg Asn Val Pro Gln 
            100                 105                 110         


Ile Glu Ser Arg Arg Lys Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe 
        115                 120                 125             


Ile Glu Gly Gly Trp Thr Gly Met Val Asp Gly Trp Tyr Gly Tyr His 
    130                 135                 140                 


His Ser Asn Glu Gln Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ser Thr 
145                 150                 155                 160 


Gln Lys Ala Ile Asp Gly Ile Thr Asn Lys Val Asn Ser Val Ile Asp 
                165                 170                 175     


Lys Met Asn Thr Gln Phe Glu Ala Val Gly Lys Glu Phe Asn Asn Leu 
            180                 185                 190         


Glu Arg Arg Ile Glu Asn Leu Asn Lys Lys Met Glu Asp Gly Phe Leu 
        195                 200                 205             


Asp Val Trp Thr Tyr Asn Ala Glu Leu Leu Val Leu Met Glu Asn Glu 
    210                 215                 220                 


Arg Thr Leu Asp Phe His Asp Ser Asn Val Lys Asn Leu Tyr Asp Lys 
225                 230                 235                 240 


Val Arg Met Gln Leu Arg Asp Asn Ala Lys Glu Leu Gly Asn Gly Cys 
                245                 250                 255     


Phe Glu Phe Tyr Lys Cys Asp Asn Glu Cys Met Glu Ser Val Lys Asn 
            260                 265                 270         


Gly Thr Tyr Asp Tyr Pro Lys Tyr Ser Glu Glu Ala Lys Leu Asn Arg 
        275                 280                 285             


Glu Lys Ile Glu Gly Val Lys Leu Glu Ser Met Gly Val Tyr Gln Ile 
    290                 295                 300                 


Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val Val Met 
305                 310                 315                 320 


Ile Ala Gly Ile Ser Phe Trp Met Cys Ser Asn Gly Ser Leu Gln Cys 
                325                 330                 335     


Arg Ile Cys Ile Ile Cys Glu Leu Arg Leu Leu 
            340                 345         


<210>  4
<211>  339
<212>  PRT
<213>  Influenza A virus

<400>  4

Met Glu Lys Phe Ile Leu Ile Ser Ile Leu Leu Ala Ala Thr Val Ala 
1               5                   10                  15      


Asn Tyr Asp Lys Ile Cys Ile Gly Tyr Gln Ser Asn Asn Ser Thr Glu 
            20                  25                  30          


Thr Val Asn Thr Leu Ile Glu Gln Asn Val Pro Val Thr Gln Ala Lys 
        35                  40                  45              


Glu Leu Val His Thr Glu Lys Asn Pro Val Leu Cys Gly Gly Gly Gly 
    50                  55                  60                  


Cys Thr Thr Gln Cys Gln Thr Glu Lys Gly Ala Leu Asn Thr Ser Lys 
65                  70                  75                  80  


Pro Phe Gln Asn Val Ser Lys His Tyr Ile Gly Lys Cys Pro Lys Tyr 
                85                  90                  95      


Ile Pro Ser Ala Ser Leu Lys Leu Ala Val Gly Leu Arg Asn Val Pro 
            100                 105                 110         


Ser Ile Gln Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu 
        115                 120                 125             


Gly Gly Trp Ser Gly Leu Val Ala Gly Trp Tyr Gly Phe Gln His Ser 
    130                 135                 140                 


Asn Ser Glu Gly Thr Gly Met Ala Ala Asp Arg Asp Ser Thr Gln Lys 
145                 150                 155                 160 


Ala Ile Asp Lys Ile Thr Asn Lys Val Asn Asn Ile Val Asp Lys Met 
                165                 170                 175     


Asn Lys Gln Phe Glu Val Val Asp His Glu Phe Ser Glu Val Glu Ser 
            180                 185                 190         


Arg Ile Asn Met Ile Asn Asn Lys Ile Asp Asp Gln Ile Gln Asp Ile 
        195                 200                 205             


Trp Ala Tyr Asn Ala Glu Leu Leu Val Leu Leu Glu Asn Gln Lys Thr 
    210                 215                 220                 


Leu Asp Glu His Asp Ala Asn Val Arg Asn Leu Tyr Asp Lys Val Lys 
225                 230                 235                 240 


Arg Ala Leu Ser Ser Asn Ala Ile Asp Thr Gly Asn Gly Cys Phe Glu 
                245                 250                 255     


Ile Leu His Lys Cys Asp Asn Gln Cys Met Glu Thr Ile Arg Asn Gly 
            260                 265                 270         


Thr Tyr Asn His Arg Glu Tyr Glu Glu Glu Ser Lys Leu Glu Arg Gln 
        275                 280                 285             


Lys Ile Asn Gly Val Lys Leu Glu Glu Asn Ser Thr Tyr Lys Ile Leu 
    290                 295                 300                 


Ser Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Ala Leu Met Ile 
305                 310                 315                 320 


Ala Gly Gly Leu Ile Phe Gly Met Gln Asn Gly Ser Cys Arg Cys Thr 
                325                 330                 335     


Phe Cys Ile 
            


<210>  5
<211>  340
<212>  PRT
<213>  Influenza A virus

<400>  5

Met Glu Val Lys Leu Leu Phe Ile Leu Ile Ile Val Tyr Gly Val Lys 
1               5                   10                  15      


Ala Asp Lys Ile Cys Ile Gly Tyr Leu Ser Asn Asn Ser Thr Asp Lys 
            20                  25                  30          


Val Asp Thr Leu Ile Glu Asn Asn Val Pro Val Thr Ser Ser Val Asp 
        35                  40                  45              


Leu Val Glu Thr Asn His Thr Gly Thr Tyr Cys Gly Gly Gly Gly Cys 
    50                  55                  60                  


Asn Thr Lys Cys Gln Thr Ser Val Gly Gly Ile Asn Thr Asn Lys Thr 
65                  70                  75                  80  


Phe Gln Asn Ile Glu Arg Asn Ala Ile Gly Asp Cys Pro Lys Tyr Ile 
                85                  90                  95      


Lys Ser Gly Gln Leu Lys Leu Ala Thr Gly Leu Arg Asn Val Pro Ser 
            100                 105                 110         


Ile Ala Ser Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Phe Ile Glu 
        115                 120                 125             


Gly Gly Trp Pro Gly Leu Ile Asn Gly Trp Tyr Gly Phe Gln His Gln 
    130                 135                 140                 


Asn Glu Gln Gly Thr Gly Ile Ala Ala Asp Lys Glu Ser Thr Gln Lys 
145                 150                 155                 160 


Ala Ile Asp Gln Ile Thr Thr Lys Ile Asn Asn Ile Ile Glu Lys Met 
                165                 170                 175     


Asn Gly Asn Tyr Asp Ser Ile Arg Gly Glu Phe Asn Gln Val Glu Glu 
            180                 185                 190         


Arg Ile Asn Met Leu Ala Asp Arg Val Asp Asp Ala Val Thr Asp Val 
        195                 200                 205             


Trp Ser Tyr Asn Ala Lys Leu Leu Val Leu Leu Glu Asn Asp Lys Thr 
    210                 215                 220                 


Leu Asp Leu His Asp Ala Asn Val Arg Asn Leu His Asp Gln Val Arg 
225                 230                 235                 240 


Arg Ala Leu Lys Asn Asn Ala Ile Asp Glu Gly Asn Gly Cys Phe Asn 
                245                 250                 255     


Leu Leu His Lys Cys Asn Asp Ser Cys Met Glu Thr Ile Arg Asn Gly 
            260                 265                 270         


Thr Tyr Asn His Lys Glu Tyr Glu Glu Glu Ser Lys Leu Lys Arg Gln 
        275                 280                 285             


Glu Ile Glu Gly Ile Lys Leu Lys Ser Glu Asp Asn Val Tyr Lys Ile 
    290                 295                 300                 


Leu Ser Ile Tyr Ser Cys Ile Ala Ser Ser Val Val Leu Val Gly Leu 
305                 310                 315                 320 


Ile Leu Ala Phe Ile Met Trp Ala Cys Ser Ser Gly Asn Cys Arg Phe 
                325                 330                 335     


Asn Ile Cys Ile 
            340 


<210>  6
<211>  339
<212>  PRT
<213>  Influenza A virus

<400>  6

Met Glu Leu Ile Val Leu Leu Ile Leu Leu Asn Pro Tyr Thr Phe Val 
1               5                   10                  15      


Leu Gly Asp Arg Ile Cys Ile Gly Tyr Gln Ala Asn Gln Asn Asn Gln 
            20                  25                  30          


Thr Val Asn Thr Leu Leu Glu Gln Asn Val Pro Val Thr Gly Ala Gln 
        35                  40                  45              


Glu Ile Leu Glu Thr Asn His Asn Gly Lys Leu Cys Gly Gly Gly Gly 
    50                  55                  60                  


Cys Ser Thr Lys Cys Gln Thr Pro Leu Gly Ala Leu Asn Ser Thr Leu 
65                  70                  75                  80  


Pro Phe Gln Asn Val His Gln Gln Thr Ile Gly Asn Cys Pro Lys Tyr 
                85                  90                  95      


Val Lys Ala Thr Ser Leu Met Leu Ala Thr Gly Leu Arg Asn Asn Pro 
            100                 105                 110         


Gln Met Glu Gly Arg Gly Leu Phe Gly Ala Ile Ala Gly Phe Ile Glu 
        115                 120                 125             


Gly Gly Trp Gln Gly Met Ile Asp Gly Trp Tyr Gly Tyr His His Glu 
    130                 135                 140                 


Asn Gln Glu Gly Ser Gly Tyr Ala Ala Asp Lys Glu Ala Thr Gln Lys 
145                 150                 155                 160 


Ala Val Asp Ala Ile Thr Asn Lys Val Asn Ser Ile Ile Asp Lys Met 
                165                 170                 175     


Asn Ser Gln Phe Glu Ser Asn Ile Lys Glu Phe Asn Arg Leu Glu Leu 
            180                 185                 190         


Arg Ile Gln His Leu Ser Asp Arg Val Asp Asp Ala Leu Leu Asp Ile 
        195                 200                 205             


Trp Ser Tyr Asn Thr Glu Leu Leu Val Leu Leu Glu Asn Glu Arg Thr 
    210                 215                 220                 


Leu Asp Phe His Asp Ala Asn Val Lys Asn Leu Phe Glu Lys Val Lys 
225                 230                 235                 240 


Ala Gln Leu Lys Asp Asn Ala Ile Asp Glu Gly Asn Gly Cys Phe Leu 
                245                 250                 255     


Leu Leu His Lys Cys Asn Asn Ser Cys Met Asp Asp Ile Lys Asn Gly 
            260                 265                 270         


Thr Tyr Lys Tyr Met Asp Tyr Arg Glu Glu Ser His Ile Glu Lys Gln 
        275                 280                 285             


Lys Ile Asp Gly Val Lys Leu Thr Asp Tyr Ser Arg Tyr Tyr Ile Met 
    290                 295                 300                 


Thr Leu Tyr Ser Thr Ile Ala Ser Ser Val Val Leu Gly Ser Leu Ile 
305                 310                 315                 320 


Ile Ala Ala Phe Leu Trp Gly Cys Gln Lys Gly Ser Ile Gln Cys Lys 
                325                 330                 335     


Ile Cys Ile 
            


