                         SEQUENCE LISTING

<110>  Merck Sharp & Dohme Corp.
       Castor, Michelle
       Chen, Ming-Tang
       Hsieh, Chung-Ming
 
<120>  FULL-LENGTH HUMAN IMMUNOGLOBULIN G ANTIBODY LIBRARIES FOR SURFACE
        DISPLAY AND SECRETION IN SACCHAROMYCES CEREVISIAE

<130>  25142

<150>  63/117,289
<151>  2020-11-23

<160>  42    

<170>  PatentIn version 3.5

<210>  1
<211>  1710
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding Fc-SED1.320 bait expression cassette (ScSED1.320 
       Anchor)

<400>  1
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgac       60

aagacacata cttgtccacc atgtccagct ccagaattgt tgggtggtcc atccgttttc      120

ttgttcccac caaagccaaa ggacactttg atgatctcca gaactccaga ggttacatgt      180

gttgttgttg acgtttctca cgaggaccca gaggttaagt tcaactggta cgttgacggt      240

gttgaagttc acaacgctaa gactaagcca agagaagagc agtacgcttc cacttacaga      300

gttgtttccg ttttgactgt tttgcaccag gactggttga acggtaaaga atacaagtgt      360

aaggtttcca acaaggcttt gccagctcca atcgaaaaga ctatctccaa ggctaagggt      420

caaccaagag agccacaggt ttacactttg ccaccatcca gagaagagat gactaagaac      480

caggtttcct tgacttgttt ggttaaagga ttctacccat ccgacattgc tgttgagtgg      540

gaatctaacg gtcaaccaga gaacaactac aagactactc caccagtttt ggattctgat      600

ggttccttct tcttgtactc caagttgact gttgacaagt ccagatggca acagggtaac      660

gttttctcct gttccgttat gcatgaggct ttgcacaacc actacactca aaagtccttg      720

tctttgtccc ctggtggtgg tggtgtcgac caattttcta attctacatc agcatcttca      780

acagacgtaa cttccagttc ttcaatatca acttccagtg gttccgtcac tatcacatct      840

tcagaagctc cagaaagtga taacggtact tctactgcag cccctacaga aacctcaact      900

gaagccccaa ccactgctat tcctactaat ggtacatcta ccgaagcacc aacaaccgcc      960

atacctacaa acggtacttc tacagaagca ccaactgata ctacaaccga agctccaact     1020

acagcattgc ctacaaatgg tacttctact gaagccccaa ctgacaccac tacagaagct     1080

ccaaccactg gtttgcctac aaacggtaca acctcagctt ttccacctac tacatcctta     1140

ccacctagta ataccactac aaccccacct tataacccat ctactgatta tactacagac     1200

tacacagttg taactgaata taccacttac tgtccagaac ctacaacctt cactacaaat     1260

ggtaaaacat acaccgttac tgaaccaacc actttaacaa taaccgattg tccatgcaca     1320

atcgaaaagc ctacaaccac ttctacaacc gaatacacag tcgttactga atacactaca     1380

tactgtccag aacctaccac tttcacaacc aatggtaaaa cttacacagt taccgaacca     1440

actacattga ctattacaga ctgtccttgc actatagaaa agtcagaagc tccagaatcc     1500

agtgtacctg tcacagaatc caaaggtact actacaaagg aaactggtgt taccactaaa     1560

caaacaaccg caaatccatc tttaacagtc tcaactgtag tccctgtttc ttcatccgcc     1620

agttctcatt cagttgtaat taattccaac ggtgctaatg ttgtcgttcc aggtgctttg     1680

ggtttggcag gtgttgctat gttgtttttg                                      1710


<210>  2
<211>  318
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TetO7 promoter DNA sequence

<400>  2
ctcgatcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac       60

tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag      120

aaaagtgaaa gtcgagttta ccactcctca gtgactatag agaaaagtga aagtcgagtt      180

taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg      240

atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt      300

cgagctcggt accctatg                                                    318


<210>  3
<211>  235
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CYC-TT DNA sequence

<400>  3
catgtaatta gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga       60

aaaggaagga gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt      120

agtattaaga acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta      180

cgcatgtaac attatactga aaaccttgct tgagaaggtt ttgggacgct cgaag           235


<210>  4
<211>  1615
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ScMET15 gene

<400>  4
atcctcatga aaactgtgta acataataac cgaagtgtcg aaaaggtggc accttgtcca       60

attgaacacg ctcgatgaaa aaaataagat atatataagg ttaagtaaag cgtctgttag      120

aaaggaagtt tttccttttt cttgctctct tgtcttttca tctactattt ccttcgtgta      180

atacagggtc gtcagataca tagatacaat tctattaccc ccatccatac aatgccatct      240

catttcgata ctgttcaact acacgccggc caagagaacc ctggtgacaa tgctcacaga      300

tccagagctg taccaattta cgccaccact tcttatgttt tcgaaaactc taagcatggt      360

tcgcaattgt ttggtctaga agttccaggt tacgtctatt cccgtttcca aaacccaacc      420

agtaatgttt tggaagaaag aattgctgct ttagaaggtg gtgctgctgc tttggctgtt      480

tcctccggtc aagccgctca aacccttgcc atccaaggtt tggcacacac tggtgacaac      540

atcgtttcca cttcttactt atacggtggt acttataacc agttcaaaat ctcgttcaaa      600

agatttggta tcgaggctag atttgttgaa ggtgacaatc cagaagaatt cgaaaaggtc      660

tttgatgaaa gaaccaaggc tgtttatttg gaaaccattg gtaatccaaa gtacaatgtt      720

ccggattttg aaaaaattgt tgcaattgct cacaaacacg gtattccagt tgtcgttgac      780

aacacatttg gtgccggtgg ttacttctgt cagccaatta aatacggtgc tgatattgta      840

acacattctg ctaccaaatg gattggtggt catggtacta ctatcggtgg tattattgtt      900

gactctggta agttcccatg gaaggactac ccagaaaagt tccctcaatt ctctcaacct      960

gccgaaggat atcacggtac tatctacaat gaagcctacg gtaacttggc atacatcgtt     1020

catgttagaa ctgaactatt aagagatttg ggtccattga tgaacccatt tgcctctttc     1080

ttgctactac aaggtgttga aacattatct ttgagagctg aaagacacgg tgaaaatgca     1140

ttgaagttag ccaaatggtt agaacaatcc ccatacgtat cttgggtttc ataccctggt     1200

ttagcatctc attctcatca tgaaaatgct aagaagtatc tatctaacgg tttcggtggt     1260

gtcttatctt tcggtgtaaa agacttacca aatgccgaca aggaaactga cccattcaaa     1320

ctttctggtg ctcaagttgt tgacaattta aagcttgcct ctaacttggc caatgttggt     1380

gatgccaaga ccttagtcat tgctccatac ttcactaccc acaaacaatt aaatgacaaa     1440

gaaaagttgg catctggtgt taccaaggac ttaattcgtg tctctgttgg tatcgaattt     1500

attgatgaca ttattgcaga cttccagcaa tcttttgaaa ctgttttcgc tggccaaaaa     1560

ccatgagtgt gcgtaatgag ttgtaaaatt atgtataaac ctactttctc tcaca          1615


<210>  5
<211>  1482
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ScURA3 gene

<400>  5
caatacagac gatgataaca aaccgaagtt atctgatgta gaaaaggatt aaagatgcta       60

agagatagtg atgatatttc ataaataatg taattctata tatgttaatt accttttttg      120

cgaggcatat ttatggtgaa ggataagttt tgaccatcaa agaaggttaa tgtggctgtg      180

gtttcagggt ccataaagct tttcaattca tctttttttt ttttgttctt ttttttgatt      240

ccggtttctt tgaaattttt ttgattcggt aatctccgag cagaaggaag aacgaaggaa      300

ggagcacaga cttagattgg tatatatacg catatgtggt gttgaagaaa catgaaattg      360

cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat      420

gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct      480

atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac      540

caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca      600

tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc      660

cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt      720

caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc      780

acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcgg aagaagtaac      840

aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctagctac      900

tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg      960

ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac     1020

acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga     1080

tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg     1140

aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag     1200

aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc     1260

acaaattaga gcttcaattt aattatatca gttattaccc gggaatctcg gtcgtaatga     1320

tttctataat gacgaaaaaa aaaaaattgg aaagaaaaag cttcatggcc tttataaaaa     1380

ggaactatcc aatacctcgc cagaaccaag taacagtatt ttacggggca caaatcaaga     1440

acaataagac aggactgtaa agatggacgc attgaactcc aa                        1482


<210>  6
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding Anti-HER2 IgG1-G1m(f), N297A expression cassette

<400>  6
atgagattcc catccatctt cactgctgtt ttgttcgctg cttcttctgc tttggctgag       60

gttcagttgg ttgaatctgg aggaggattg gttcaacctg gtggttcttt gagattgtcc      120

tgtgctgctt ccggtttcaa catcaaggac acttacatcc actgggttag acaagctcca      180

ggaaagggat tggagtgggt tgctagaatc tacccaacta acggttacac aagatacgct      240

gactccgtta agggaagatt cactatctct gctgacactt ccaagaacac tgcttacttg      300

cagatgaact ccttgagagc tgaggatact gctgtttact actgttccag atggggtggt      360

gatggtttct acgctatgga ctactggggt caaggaactt tggttactgt ctcgagtgct      420

tcaactaagg gaccatctgt cttcccattg gctccatctt caaagtctac ttcaggtggt      480

actgctgctt tgggttgctt ggtcaaagac tacttcccag agccagtcac agtttcttgg      540

aactctggtg ctttgacttc tggtgtccac actttcccag cagtcttaca atcttctggt      600

ttgtattcat tgtcttctgt tgttacagtt ccatcttctt ctttgggtac tcaaacttat      660

atttgtaatg ttaaccataa accatctaat actaaagttg ataagaaagt tgaaccaaaa      720

tcttgtgata agactcacac ttgtccacct tgcccagctc cagaattgtt aggtggtcct      780

tctgtcttct tgttcccacc aaagccaaaa gatacattga tgatttctag aactccagag      840

gttacatgtg tcgtcgtcga tgtctctcac gaggatcctg aggtcaagtt caactggtac      900

gtcgacggtg ttgaggtcca caacgctaag actaagccaa gagaagaaca atacgcttct      960

acatatagag ttgtctctgt tttgactgtt ttgcatcagg attggttaaa tggtaaagaa     1020

tataagtgta aagtttctaa taaggcttta ccagcaccaa ttgaaaaaac tatttctaag     1080

gctaagggtc aaccaaggga accacaggtc tacacattgc caccatcaag agaagaaatg     1140

actaaaaatc aagtttcttt aacttgcttg gttaagggtt tctatccatc agatattgct     1200

gtcgagtggg aatctaatgg tcagccagaa aataattata aaacaactcc accagttttg     1260

gattctgatg gttctttctt tttatattct aaattgacag tcgataagtc taggtggcag     1320

caaggtaacg ttttctcatg ctctgtcatg cacgaggctt tgcacaacca ctacacacaa     1380

aaatctttgt cattatctcc aggt                                            1404


<210>  7
<211>  456
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gal1 promoter DNA sequence

<400>  7
tgaagtacgg attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc       60

gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg      120

ctccgaacaa taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc      180

agtaacctgg ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata      240

atgcgattag ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg      300

atctattaac agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac      360

attttcggtt tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta      420

atatacctct atactttaac gtcaaggaga aaaaac                                456


<210>  8
<211>  1103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ScLEU2 gene

<400>  8
atgtctgccc ctatgtctgc ccctaagaag atcgtcgttt tgccaggtga ccacgttggt       60

caagaaatca cagccgaagc cattaaggtt cttaaagcta tttctgatgt tcgttccaat      120

gtcaagttcg atttcgaaaa tcatttaatt ggtggtgctg ctatcgatgc tacaggtgtc      180

ccacttccag atgaggcgct ggaagcctcc aagaaggttg atgccgtttt gttaggtgct      240

gtgggtggtc ctaaatgggg taccggtagt gttagacctg aacaaggttt actaaaaatc      300

cgtaaagaac ttcaattgta cgccaactta agaccatgta actttgcatc cgactctctt      360

ttagacttat ctccaatcaa gccacaattt gctaaaggta ctgacttcgt tgttgtcaga      420

gaattagtgg gaggtattta ctttggtaag agaaaggaag acgatggtga tggtgtcgct      480

tgggatagtg aacaatacac cgttccagaa gtgcaaagaa tcacaagaat ggccgctttc      540

atggccctac aacatgagcc accattgcct atttggtcct tggataaagc taatgttttg      600

gcctcttcaa gattatggag aaaaactgtg gaggaaacca tcaagaacga atttcctaca      660

ttgaaggttc aacatcaatt gattgattct gccgccatga tcctagttaa gaacccaacc      720

cacctaaatg gtattataat caccagcaac atgtttggtg atatcatctc cgatgaagcc      780

tccgttatcc caggttcctt gggtttgttg ccatctgcgt ccttggcctc tttgccagac      840

aagaacaccg catttggttt gtacgaacca tgccacggtt ctgctccaga tttgccaaag      900

aataaggtca accctatcgc cactatcttg tctgctgcaa tgatgttgaa attgtcattg      960

aacttgcctg aagaaggtaa ggccattgaa gatgcagtta aaaaggtttt ggatgcaggt     1020

atcagaactg gtgatttagg tggttccaac agtaccaccg aagtcggtga tgctgtcgcc     1080

gaagaagtta agaaaatcct tgc                                             1103


<210>  9
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding MF-Pre-anti-HER2-Lc Kappa CL expression cassette

<400>  9
atgagattcc catccatctt caccgccgtt ttgttcgctg cttcttccgc tttggctgat       60

attcaaatga ctcaatctcc atcttctttg tctgcttctg tcggtgatcg tgtcactatt      120

acttgtcgtg cttctcaaga tgtcaacact gctgtcgctt ggtatcaaca gaagcccggt      180

aaggctccaa agttgttgat ttattctgct tcttttttgt actctggtgt cccatctagg      240

ttctctggtt ctaggtctgg tactgatttt actttgacta tttcttcttt gcaaccagaa      300

gattttgcta cttattattg tcaacaacat tatactacac caccaacttt tggtcaaggt      360

accaaggttg agatcaagag aactgttgct gctccatccg ttttcatttt cccaccatcc      420

gacgaacagt tgaagtctgg tacagcttcc gttgtttgtt tgttgaacaa cttctaccca      480

agagaggcta aggttcagtg gaaggttgac aacgctttgc aatccggtaa ctcccaagaa      540

tccgttactg agcaagactc taaggactcc acttactcct tgtcctccac tttgactttg      600

tccaaggctg attacgagaa gcacaaggtt tacgcttgtg aggttacaca tcagggtttg      660

tcctccccag ttactaagtc cttcaacaga ggagagtgt                             699


<210>  10
<211>  672
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TRP1 gene

<400>  10
atgtctgtta ttaatttcac aggtagttct ggtccattgg tgaaagtttg cggcttgcag       60

agcacagagg ccgcagaatg tgctctagat tccgatgctg acttgctggg tattatatgt      120

gtgcccaata gaaagagaac aattgacccg gttattgcaa ggaaaatttc aagtcttgta      180

aaagcatata aaaatagttc aggcactccg aaatacttgg ttggcgtgtt tcgtaatcaa      240

cctaaggagg atgttttggc tctggtcaat gattacggca ttgatatcgt ccaactgcat      300

ggagatgagt cgtggcaaga ataccaagag ttcctcggtt tgccagttat taaaagactc      360

gtatttccaa aagactgcaa catactactc agtgcagctt cacagaaacc tcattcgttt      420

attcccttgt ttgattcaga agcaggtggg acaggtgaac ttttggattg gaactcgatt      480

tctgactggg ttggaaggca agagagcccc gaaagcttac attttatgtt agctggtgga      540

ctgacgccag aaaatgttgg tgatgcgctt agattaaatg gcgttattgg tgttgatgta      600

agcggaggtg tggagacaaa tggtgtaaaa gactctaaca aaatagcaaa tttcgtcaaa      660

aatgctaaga aa                                                          672


<210>  11
<211>  2175
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding AGA1

<400>  11
atgactttgt ctttcgcaca tttcacttat ttatttacta ttttgttggg attgactaat       60

attgctttgg cttctgatcc agaaactatt ttggttacta ttactaaaac taacgatgct      120

aacggagtcg tcacaactac tgtctcacca gctttggtct ctacttctac tattgtccaa      180

gctggtacaa ctactttgta tactacttgg tgtccattaa ctgtttctac ttcatcagca      240

gcagaaattt ctccatctat ttcatacgct acaacattgt caagattttc aactttaaca      300

ttgtcaactg aggtttgctc acatgaggct tgcccttctt catctacttt acctactaca      360

acattgtcag ttacttcaaa gttcacttct tacatttgcc caacatgtca tactacagct      420

atttcttcat tgtctgaagt cggtactaca actgttgttt catcttctgc tatagaacca      480

tcttcagctt ctattatatc tccagttaca tctactttgt cttcaacaac ttcttctaac      540

cctacaacta cttctttgtc atcaacatct acttctccat cttcaacttc tacttctcca      600

tcatctacat ctacttcatc ttcttctact tctacttcat cttcttctac ttcaacttca      660

tcttcttcta cttctacttc tccatcatct acatcaacat catcatcttt gacatctaca      720

tcttcttctt caacatctac ttcacagtct tctacttcta catcttcttc atcaacttca      780

acatctcctt cttcaacatc tacatcttct tcttcaacat caacatcacc atcatctaaa      840

tcaacttcag cttcatctac ttctacatct tcttattcta cttctacttc tccatcattg      900

acttcttctt caccaacatt ggcttctaca tcaccatctt ctacttctat atcttctaca      960

tttactgatt caacttcttc tttgggttca tcaatagctt cttcttcaac atctgtttct     1020

ttgtactctc catctactcc cgtttactca gtcccttcta catcatctaa cgttgctact     1080

ccttctatga catcttctac tgtcgagact actgtttcat ctcaatcatc ttctgaatat     1140

attactaaat cttcaatttc tactactatt ccatcttttt ctatgtcaac atatttcact     1200

acagtttctg gtgttacaac tatgtacact acttggtgcc catactcttc agaatctgaa     1260

acttcaacat tgacttctat gcatgaaaca gtcacaactg acgctactgt ttgcacacac     1320

gagtcttgca tgccatctca gactacttca ttaattactt cttctattaa aatgtctact     1380

aaaaatgttg ctacttctgt ttcaacatct acagttgaat cttcttacgc ttgttctact     1440

tgcgctgaaa cttcacattc atattcttca gttcaaactg cttcttcttc ttctgttact     1500

cagcaaacaa cttcaacaaa atcatgggtc tcatctatga caacatctga tgaagacttt     1560

aacaaacacg ctactggtaa atatcatgtt acttcttctg gaacttctac aatttctaca     1620

tctgtttctg aagctacatc tacttcttct atagattctg agtctcaaga acaatcttct     1680

cacttattat caacatctgt cttgtcttct tcttctttat ctgctacatt gtcatctgac     1740

tcaactattt tgttattctc ttcagtctct tctttgtcag ttgaacaatc tccagtcact     1800

actttgcaaa tatcatcaac ttctgagatt ttacaaccaa catcttctac agctatagct     1860

actatttctg cttctacttc ttctttatct gctacttcta tatctacacc atctacatct     1920

gttgaatcta ctattgagtc ttcttctttg acacctactg tttcttctat tttcttatct     1980

tcttcttctg ctccatcttc tttgcaaaca tctgttacaa ctactgaagt ttctactaca     2040

tctatttcaa ttcaatatca gacttcttct atggtcacaa tttctcaata catgggttct     2100

ggttctcaaa ctagattgcc attgggtaaa ttggtttttg ctattatggc agtcgcttgt     2160

aatgttattt tctct                                                      2175


<210>  12
<211>  993
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding Fc-AGA2 bait expression cassette

<400>  12
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgac       60

aagacacata cttgtccacc atgtccagct ccagaattgt tgggtggtcc atccgttttc      120

ttgttcccac caaagccaaa ggacactttg atgatctcca gaactccaga ggttacatgt      180

gttgttgttg acgtttctca cgaggaccca gaggttaagt tcaactggta cgttgacggt      240

gttgaagttc acaacgctaa gactaagcca agagaagagc agtacgcttc cacttacaga      300

gttgtttccg ttttgactgt tttgcaccag gactggttga acggtaaaga atacaagtgt      360

aaggtttcca acaaggcttt gccagctcca atcgaaaaga ctatctccaa ggctaagggt      420

caaccaagag agccacaggt ttacactttg ccaccatcca gagaagagat gactaagaac      480

caggtttcct tgacttgttt ggttaaagga ttctacccat ccgacattgc tgttgagtgg      540

gaatctaacg gtcaaccaga gaacaactac aagactactc caccagtttt ggattctgat      600

ggttccttct tcttgtactc caagttgact gttgacaagt ccagatggca acagggtaac      660

gttttctcct gttccgttat gcatgaggct ttgcacaacc actacactca aaagtccttg      720

tctttgtccc ctaaagctag cggtggtggt ggttctgaag ctgcagcaaa agaggcagct      780

gctaagcaag agttgactac tatttgtgaa caaattccat ctccaacttt ggaatctact      840

ccatattctt tgtctactac tactattttg gctaatggta aagctatgca aggtgttttt      900

gaatattata aatctgttac tttcgtctct aactgcggtt ctcacccatc tactacttct      960

aagggttctc caattaatac tcaatatgtt ttt                                   993


<210>  13
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.320 (1-18 SED1 signal sequence)

<400>  13

Met Lys Leu Ser Thr Val Leu Leu Ser Ala Gly Leu Ala Ser Thr Thr 
1               5                   10                  15      


Leu Ala Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr 
            20                  25                  30          


Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser 
        35                  40                  45              


Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr 
    50                  55                  60                  


Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr 
65                  70                  75                  80  


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
                85                  90                  95      


Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro 
            100                 105                 110         


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala 
        115                 120                 125             


Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro 
    130                 135                 140                 


Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn 
145                 150                 155                 160 


Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr 
                165                 170                 175     


Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr 
            180                 185                 190         


Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr 
        195                 200                 205             


Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr 
    210                 215                 220                 


Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly 
225                 230                 235                 240 


Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys 
                245                 250                 255     


Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val 
            260                 265                 270         


Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys 
        275                 280                 285             


Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val 
    290                 295                 300                 


Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala 
305                 310                 315                 320 


Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu 
                325                 330                 335     


Phe Leu 
        


<210>  14
<211>  419
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.401 (1-18 SED1 signal sequence)

<400>  14

Met Lys Leu Ser Thr Val Leu Leu Ser Ala Gly Leu Ala Ser Thr Thr 
1               5                   10                  15      


Leu Ala Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr 
            20                  25                  30          


Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser 
        35                  40                  45              


Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr 
    50                  55                  60                  


Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr 
65                  70                  75                  80  


Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala 
                85                  90                  95      


Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr 
            100                 105                 110         


Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala 
        115                 120                 125             


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn 
    130                 135                 140                 


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
145                 150                 155                 160 


Thr Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr 
                165                 170                 175     


Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser 
            180                 185                 190         


Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr 
        195                 200                 205             


Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val 
    210                 215                 220                 


Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu 
225                 230                 235                 240 


Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr 
                245                 250                 255     


Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr 
            260                 265                 270         


Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys 
        275                 280                 285             


Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val 
    290                 295                 300                 


Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn 
305                 310                 315                 320 


Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp 
                325                 330                 335     


Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro 
            340                 345                 350         


Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr 
        355                 360                 365             


Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro 
    370                 375                 380                 


Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly 
385                 390                 395                 400 


Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met 
                405                 410                 415     


Leu Phe Leu 
            


<210>  15
<211>  448
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.430 (1-18 SED1 signal sequence)

<400>  15

Met Lys Leu Ser Thr Val Leu Leu Ser Ala Gly Leu Ala Ser Thr Thr 
1               5                   10                  15      


Leu Ala Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr 
            20                  25                  30          


Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser 
        35                  40                  45              


Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr 
    50                  55                  60                  


Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr 
65                  70                  75                  80  


Ser Thr Glu Ala Pro Thr Asp Thr Thr Ser Glu Ala Pro Thr Thr Ala 
                85                  90                  95      


Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr 
            100                 105                 110         


Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala 
        115                 120                 125             


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn 
    130                 135                 140                 


Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn 
145                 150                 155                 160 


Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp 
                165                 170                 175     


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
            180                 185                 190         


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
        195                 200                 205             


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser 
    210                 215                 220                 


Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu 
225                 230                 235                 240 


Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro 
                245                 250                 255     


Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr 
            260                 265                 270         


Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr 
        275                 280                 285             


Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val 
    290                 295                 300                 


Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu 
305                 310                 315                 320 


Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr 
                325                 330                 335     


Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr 
            340                 345                 350         


Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys 
        355                 360                 365             


Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr Glu 
    370                 375                 380                 


Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln Thr 
385                 390                 395                 400 


Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser Ser 
                405                 410                 415     


Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn Val 
            420                 425                 430         


Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
        435                 440                 445             


<210>  16
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.481 (1-18 SED1 signal sequence)

<400>  16

Met Lys Leu Ser Thr Val Leu Leu Ser Ala Gly Leu Ala Ser Thr Thr 
1               5                   10                  15      


Leu Ala Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Ala Thr 
            20                  25                  30          


Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser 
        35                  40                  45              


Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr 
    50                  55                  60                  


Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr 
65                  70                  75                  80  


Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala 
                85                  90                  95      


Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr 
            100                 105                 110         


Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala 
        115                 120                 125             


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn 
    130                 135                 140                 


Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn 
145                 150                 155                 160 


Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp 
                165                 170                 175     


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
            180                 185                 190         


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
        195                 200                 205             


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser 
    210                 215                 220                 


Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu 
225                 230                 235                 240 


Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro 
                245                 250                 255     


Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr 
            260                 265                 270         


Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr 
        275                 280                 285             


Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val 
    290                 295                 300                 


Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu 
305                 310                 315                 320 


Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr 
                325                 330                 335     


Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr 
            340                 345                 350         


Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys 
        355                 360                 365             


Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val 
    370                 375                 380                 


Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn 
385                 390                 395                 400 


Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp 
                405                 410                 415     


Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro 
            420                 425                 430         


Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr 
        435                 440                 445             


Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro 
    450                 455                 460                 


Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly 
465                 470                 475                 480 


Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met 
                485                 490                 495     


Leu Phe Leu 
            


<210>  17
<211>  320
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.320 (without SED1 signal sequence)

<400>  17

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala 
65                  70                  75                  80  


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn 
                85                  90                  95      


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
            100                 105                 110         


Thr Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr 
        115                 120                 125             


Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser 
    130                 135                 140                 


Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr 
145                 150                 155                 160 


Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val 
                165                 170                 175     


Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu 
            180                 185                 190         


Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr 
        195                 200                 205             


Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr 
    210                 215                 220                 


Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys 
225                 230                 235                 240 


Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr Glu 
                245                 250                 255     


Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln Thr 
            260                 265                 270         


Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser Ser 
        275                 280                 285             


Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn Val 
    290                 295                 300                 


Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
305                 310                 315                 320 


<210>  18
<211>  401
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.401 (without SED1 signal sequence)

<400>  18

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro 
65                  70                  75                  80  


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala 
                85                  90                  95      


Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr 
            100                 105                 110         


Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr 
        115                 120                 125             


Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Gly 
    130                 135                 140                 


Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu 
145                 150                 155                 160 


Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp 
                165                 170                 175     


Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro 
            180                 185                 190         


Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu 
        195                 200                 205             


Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro 
    210                 215                 220                 


Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr 
225                 230                 235                 240 


Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr 
                245                 250                 255     


Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile 
            260                 265                 270         


Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu 
        275                 280                 285             


Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys 
    290                 295                 300                 


Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro 
305                 310                 315                 320 


Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr 
                325                 330                 335     


Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln 
            340                 345                 350         


Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser 
        355                 360                 365             


Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn 
    370                 375                 380                 


Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe 
385                 390                 395                 400 


Leu 
    


<210>  19
<211>  430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.430 (without SED1 signal sequence)

<400>  19

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Asp Thr Thr Ser Glu Ala Pro Thr Thr Ala Leu Pro 
65                  70                  75                  80  


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala 
                85                  90                  95      


Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr 
            100                 105                 110         


Asp Thr Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr 
        115                 120                 125             


Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr 
    130                 135                 140                 


Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr 
145                 150                 155                 160 


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
                165                 170                 175     


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
            180                 185                 190         


Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr 
        195                 200                 205             


Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
    210                 215                 220                 


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
225                 230                 235                 240 


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr 
                245                 250                 255     


Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro 
            260                 265                 270         


Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu 
        275                 280                 285             


Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro 
    290                 295                 300                 


Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr 
305                 310                 315                 320 


Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr 
                325                 330                 335     


Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile 
            340                 345                 350         


Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr Glu Ser Lys 
        355                 360                 365             


Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln Thr Thr Ala 
    370                 375                 380                 


Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser Ser Ser Ala 
385                 390                 395                 400 


Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn Val Val Val 
                405                 410                 415     


Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
            420                 425                 430 


<210>  20
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.481 (without SED1 signal sequence)

<400>  20

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Ala Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro 
65                  70                  75                  80  


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala 
                85                  90                  95      


Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr 
            100                 105                 110         


Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr 
        115                 120                 125             


Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr 
    130                 135                 140                 


Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr 
145                 150                 155                 160 


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
                165                 170                 175     


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
            180                 185                 190         


Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr 
        195                 200                 205             


Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
    210                 215                 220                 


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
225                 230                 235                 240 


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr 
                245                 250                 255     


Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro 
            260                 265                 270         


Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu 
        275                 280                 285             


Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro 
    290                 295                 300                 


Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr 
305                 310                 315                 320 


Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr 
                325                 330                 335     


Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile 
            340                 345                 350         


Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu 
        355                 360                 365             


Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys 
    370                 375                 380                 


Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro 
385                 390                 395                 400 


Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr 
                405                 410                 415     


Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln 
            420                 425                 430         


Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser 
        435                 440                 445             


Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn 
    450                 455                 460                 


Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe 
465                 470                 475                 480 


Leu 
    


<210>  21
<211>  735
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DNA encoding human IgG1 Fc N297A mutein with S. cerevisiae 
       pre-pro alpha-mating factor signal sequence at the 5' end

<400>  21

Ala Thr Gly Ala Gly Ala Thr Thr Thr Cys Cys Thr Thr Cys Ala Ala 
1               5                   10                  15      


Thr Thr Thr Thr Thr Ala Cys Thr Gly Cys Thr Gly Thr Thr Thr Thr 
            20                  25                  30          


Ala Thr Thr Cys Gly Cys Ala Gly Cys Ala Thr Cys Cys Thr Cys Cys 
        35                  40                  45              


Gly Cys Ala Thr Thr Ala Gly Cys Thr Gly Ala Cys Ala Ala Gly Ala 
    50                  55                  60                  


Cys Ala Cys Ala Thr Ala Cys Thr Thr Gly Thr Cys Cys Ala Cys Cys 
65                  70                  75                  80  


Ala Thr Gly Thr Cys Cys Ala Gly Cys Thr Cys Cys Ala Gly Ala Ala 
                85                  90                  95      


Thr Thr Gly Thr Thr Gly Gly Gly Thr Gly Gly Thr Cys Cys Ala Thr 
            100                 105                 110         


Cys Cys Gly Thr Thr Thr Thr Cys Thr Thr Gly Thr Thr Cys Cys Cys 
        115                 120                 125             


Ala Cys Cys Ala Ala Ala Gly Cys Cys Ala Ala Ala Gly Gly Ala Cys 
    130                 135                 140                 


Ala Cys Thr Thr Thr Gly Ala Thr Gly Ala Thr Cys Thr Cys Cys Ala 
145                 150                 155                 160 


Gly Ala Ala Cys Thr Cys Cys Ala Gly Ala Gly Gly Thr Thr Ala Cys 
                165                 170                 175     


Ala Thr Gly Thr Gly Thr Thr Gly Thr Thr Gly Thr Thr Gly Ala Cys 
            180                 185                 190         


Gly Thr Thr Thr Cys Thr Cys Ala Cys Gly Ala Gly Gly Ala Cys Cys 
        195                 200                 205             


Cys Ala Gly Ala Gly Gly Thr Thr Ala Ala Gly Thr Thr Cys Ala Ala 
    210                 215                 220                 


Cys Thr Gly Gly Thr Ala Cys Gly Thr Thr Gly Ala Cys Gly Gly Thr 
225                 230                 235                 240 


Gly Thr Thr Gly Ala Ala Gly Thr Thr Cys Ala Cys Ala Ala Cys Gly 
                245                 250                 255     


Cys Thr Ala Ala Gly Ala Cys Thr Ala Ala Gly Cys Cys Ala Ala Gly 
            260                 265                 270         


Ala Gly Ala Ala Gly Ala Gly Cys Ala Gly Thr Ala Cys Gly Cys Thr 
        275                 280                 285             


Thr Cys Cys Ala Cys Thr Thr Ala Cys Ala Gly Ala Gly Thr Thr Gly 
    290                 295                 300                 


Thr Thr Thr Cys Cys Gly Thr Thr Thr Thr Gly Ala Cys Thr Gly Thr 
305                 310                 315                 320 


Thr Thr Thr Gly Cys Ala Cys Cys Ala Gly Gly Ala Cys Thr Gly Gly 
                325                 330                 335     


Thr Thr Gly Ala Ala Cys Gly Gly Thr Ala Ala Ala Gly Ala Ala Thr 
            340                 345                 350         


Ala Cys Ala Ala Gly Thr Gly Thr Ala Ala Gly Gly Thr Thr Thr Cys 
        355                 360                 365             


Cys Ala Ala Cys Ala Ala Gly Gly Cys Thr Thr Thr Gly Cys Cys Ala 
    370                 375                 380                 


Gly Cys Thr Cys Cys Ala Ala Thr Cys Gly Ala Ala Ala Ala Gly Ala 
385                 390                 395                 400 


Cys Thr Ala Thr Cys Thr Cys Cys Ala Ala Gly Gly Cys Thr Ala Ala 
                405                 410                 415     


Gly Gly Gly Thr Cys Ala Ala Cys Cys Ala Ala Gly Ala Gly Ala Gly 
            420                 425                 430         


Cys Cys Ala Cys Ala Gly Gly Thr Thr Thr Ala Cys Ala Cys Thr Thr 
        435                 440                 445             


Thr Gly Cys Cys Ala Cys Cys Ala Thr Cys Cys Ala Gly Ala Gly Ala 
    450                 455                 460                 


Ala Gly Ala Gly Ala Thr Gly Ala Cys Thr Ala Ala Gly Ala Ala Cys 
465                 470                 475                 480 


Cys Ala Gly Gly Thr Thr Thr Cys Cys Thr Thr Gly Ala Cys Thr Thr 
                485                 490                 495     


Gly Thr Thr Thr Gly Gly Thr Thr Ala Ala Ala Gly Gly Ala Thr Thr 
            500                 505                 510         


Cys Thr Ala Cys Cys Cys Ala Thr Cys Cys Gly Ala Cys Ala Thr Thr 
        515                 520                 525             


Gly Cys Thr Gly Thr Thr Gly Ala Gly Thr Gly Gly Gly Ala Ala Thr 
    530                 535                 540                 


Cys Thr Ala Ala Cys Gly Gly Thr Cys Ala Ala Cys Cys Ala Gly Ala 
545                 550                 555                 560 


Gly Ala Ala Cys Ala Ala Cys Thr Ala Cys Ala Ala Gly Ala Cys Thr 
                565                 570                 575     


Ala Cys Thr Cys Cys Ala Cys Cys Ala Gly Thr Thr Thr Thr Gly Gly 
            580                 585                 590         


Ala Thr Thr Cys Thr Gly Ala Thr Gly Gly Thr Thr Cys Cys Thr Thr 
        595                 600                 605             


Cys Thr Thr Cys Thr Thr Gly Thr Ala Cys Thr Cys Cys Ala Ala Gly 
    610                 615                 620                 


Thr Thr Gly Ala Cys Thr Gly Thr Thr Gly Ala Cys Ala Ala Gly Thr 
625                 630                 635                 640 


Cys Cys Ala Gly Ala Thr Gly Gly Cys Ala Ala Cys Ala Gly Gly Gly 
                645                 650                 655     


Thr Ala Ala Cys Gly Thr Thr Thr Thr Cys Thr Cys Cys Thr Gly Thr 
            660                 665                 670         


Thr Cys Cys Gly Thr Thr Ala Thr Gly Cys Ala Thr Gly Ala Gly Gly 
        675                 680                 685             


Cys Thr Thr Thr Gly Cys Ala Cys Ala Ala Cys Cys Ala Cys Thr Ala 
    690                 695                 700                 


Cys Ala Cys Thr Cys Ala Ala Ala Ala Gly Thr Cys Cys Thr Thr Gly 
705                 710                 715                 720 


Thr Cys Thr Thr Thr Gly Thr Cys Cys Cys Cys Thr Gly Gly Thr 
                725                 730                 735 


<210>  22
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 Fc N297A mutein (C-terminal K-) with 1-19 S. 
       cerevisiae pre-pro alpha-mating factor signal sequence at the 5' 
       end

<400>  22

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            20                  25                  30          


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        35                  40                  45              


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    50                  55                  60                  


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
65                  70                  75                  80  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            100                 105                 110         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        115                 120                 125             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    130                 135                 140                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
145                 150                 155                 160 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                165                 170                 175     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            180                 185                 190         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        195                 200                 205             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    210                 215                 220                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
225                 230                 235                 240 


Ser Leu Ser Pro Gly 
                245 


<210>  23
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  23

Gly Gly Gly Val Asp 
1               5   


<210>  24
<211>  250
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 Fc N297A mutein (C-terminal K-) with GGGVD linker and 
       1-19 S. cerevisiae pre-pro alpha- mating factor signal sequence

<400>  24

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            20                  25                  30          


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        35                  40                  45              


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    50                  55                  60                  


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
65                  70                  75                  80  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            100                 105                 110         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        115                 120                 125             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    130                 135                 140                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
145                 150                 155                 160 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                165                 170                 175     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            180                 185                 190         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        195                 200                 205             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    210                 215                 220                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
225                 230                 235                 240 


Ser Leu Ser Pro Gly Gly Gly Gly Val Asp 
                245                 250 


<210>  25
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae pre-pro alpha- mating factor signal sequence

<400>  25

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala 
            


<210>  26
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human IgG1 Fc N297A mutein (C-terminal K-) without signal 
       sequence

<400>  26

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly 
225     


<210>  27
<211>  551
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.320 IgG1 Fc N297A mutein (without signal sequence) 
       (ScSED1.320 Anchor)

<400>  27

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Gly Gly Gly Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser 
225                 230                 235                 240 


Ser Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser 
                245                 250                 255     


Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser 
            260                 265                 270         


Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile 
        275                 280                 285             


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr 
    290                 295                 300                 


Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro 
305                 310                 315                 320 


Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp 
                325                 330                 335     


Thr Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr 
            340                 345                 350         


Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr 
        355                 360                 365             


Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val 
    370                 375                 380                 


Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr 
385                 390                 395                 400 


Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr 
                405                 410                 415     


Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu 
            420                 425                 430         


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
        435                 440                 445             


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
    450                 455                 460                 


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu 
465                 470                 475                 480 


Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr 
                485                 490                 495     


Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser 
            500                 505                 510         


Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile 
        515                 520                 525             


Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala 
    530                 535                 540                 


Gly Val Ala Met Leu Phe Leu 
545                 550     


<210>  28
<211>  632
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.401 IgG1 Fc N297A mutein (without signal sequence) 
       (ScSED1.401 Anchor)

<400>  28

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Gly Gly Gly Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser 
225                 230                 235                 240 


Ser Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser 
                245                 250                 255     


Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser 
            260                 265                 270         


Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile 
        275                 280                 285             


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu 
    290                 295                 300                 


Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro 
305                 310                 315                 320 


Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly 
                325                 330                 335     


Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr 
            340                 345                 350         


Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr 
        355                 360                 365             


Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala 
    370                 375                 380                 


Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro 
385                 390                 395                 400 


Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr 
                405                 410                 415     


Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly 
            420                 425                 430         


Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys 
        435                 440                 445             


Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr 
    450                 455                 460                 


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
465                 470                 475                 480 


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
                485                 490                 495     


Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr 
            500                 505                 510         


Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
        515                 520                 525             


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
    530                 535                 540                 


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro 
545                 550                 555                 560 


Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu 
                565                 570                 575     


Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val 
            580                 585                 590         


Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val 
        595                 600                 605             


Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu 
    610                 615                 620                 


Ala Gly Val Ala Met Leu Phe Leu 
625                 630         


<210>  29
<211>  661
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.430 IgG1 Fc N297A mutein (without signal sequence) 
       (ScSED1.430 Anchor)

<400>  29

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Gly Gly Gly Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser 
225                 230                 235                 240 


Ser Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser 
                245                 250                 255     


Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser 
            260                 265                 270         


Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile 
        275                 280                 285             


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Ser Glu 
    290                 295                 300                 


Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro 
305                 310                 315                 320 


Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly 
                325                 330                 335     


Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr 
            340                 345                 350         


Gly Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser 
        355                 360                 365             


Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr 
    370                 375                 380                 


Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys 
385                 390                 395                 400 


Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr 
                405                 410                 415     


Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys 
            420                 425                 430         


Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr 
        435                 440                 445             


Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr 
    450                 455                 460                 


Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr 
465                 470                 475                 480 


Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr 
                485                 490                 495     


Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly 
            500                 505                 510         


Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys 
        515                 520                 525             


Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr 
    530                 535                 540                 


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
545                 550                 555                 560 


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
                565                 570                 575     


Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser 
            580                 585                 590         


Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val 
        595                 600                 605             


Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val 
    610                 615                 620                 


Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser 
625                 630                 635                 640 


Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val 
                645                 650                 655     


Ala Met Leu Phe Leu 
            660     


<210>  30
<211>  712
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ScSED1.481 IgG1 Fc N297A mutein (without signal sequence) 
       (ScSED1.481 Anchor)

<400>  30

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Gly Gly Gly Val Asp Gln Phe Ser Asn Ser Thr Ser Ala Ser 
225                 230                 235                 240 


Ser Thr Asp Ala Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser 
                245                 250                 255     


Val Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser 
            260                 265                 270         


Thr Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile 
        275                 280                 285             


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu 
    290                 295                 300                 


Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro 
305                 310                 315                 320 


Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn Gly 
                325                 330                 335     


Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr 
            340                 345                 350         


Ala Leu Pro Thr Asn Gly Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser 
        355                 360                 365             


Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr 
    370                 375                 380                 


Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys 
385                 390                 395                 400 


Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr 
                405                 410                 415     


Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys 
            420                 425                 430         


Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr 
        435                 440                 445             


Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr 
    450                 455                 460                 


Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr 
465                 470                 475                 480 


Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr 
                485                 490                 495     


Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly 
            500                 505                 510         


Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys 
        515                 520                 525             


Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr 
    530                 535                 540                 


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
545                 550                 555                 560 


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
                565                 570                 575     


Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr 
            580                 585                 590         


Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
        595                 600                 605             


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
    610                 615                 620                 


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro 
625                 630                 635                 640 


Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu 
                645                 650                 655     


Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val 
            660                 665                 670         


Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val 
        675                 680                 685             


Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu 
    690                 695                 700                 


Ala Gly Val Ala Met Leu Phe Leu 
705                 710         


<210>  31
<211>  2229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding ScSED1.481 IgG1 Fc N297A mutein (with 1-19 S. 
       cerevisiae pre-pro alpha-mating factor signal sequence) 
       (ScSED1.481 Anchor)

<400>  31
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgac       60

aagacacata cttgtccacc atgtccagct ccagaattgt tgggtggtcc atccgttttc      120

ttgttcccac caaagccaaa ggacactttg atgatctcca gaactccaga ggttacatgt      180

gttgttgttg acgtttctca cgaggaccca gaggttaagt tcaactggta cgttgacggt      240

gttgaagttc acaacgctaa gactaagcca agagaagagc agtacgcttc cacttacaga      300

gttgtttccg ttttgactgt tttgcaccag gactggttga acggtaaaga atacaagtgt      360

aaggtttcca acaaggcttt gccagctcca atcgaaaaga ctatctccaa ggctaagggt      420

caaccaagag agccacaggt ttacactttg ccaccatcca gagaagagat gactaagaac      480

caggtttcct tgacttgttt ggttaaagga ttctacccat ccgacattgc tgttgagtgg      540

gaatctaacg gtcaaccaga gaacaactac aagactactc caccagtttt ggattctgat      600

ggttccttct tcttgtactc caagttgact gttgacaagt ccagatggca acagggtaac      660

gttttctcct gttccgttat gcatgaggct ttgcacaacc actacactca aaagtccttg      720

tctttgtccc ctaaagctag cggtggtggt ggttctgaag cagctgcaaa agaagctgct      780

gctaagcaat tttctaattc tacttctgca tcatcaacag acgctacttc ttcttcttct      840

atttctactt cttctggttc tgttactatt acttcttctg aggcaccaga gtctgacaac      900

ggtacatcta ctgctgctcc aacagagaca tccaccgaag caccaactac tgctataccc      960

actaatggta cttctactga agctccaact gatacaacta ctgaggctcc taccaccgcc     1020

ttgccaacaa acggtacttc aactgaggct ccaacagaca caaccactga agcacctact     1080

acagctttgc caactaacgg tacctctaca gaggccccaa ctgacactac aactgaggca     1140

cccactactg ccttaccaac taatggaact acttctgctt tcccaccaac tacttctttg     1200

ccaccatcta acactactac aacaccacca tataacccat ccactgatta cactactgac     1260

tacactgttg tcactgaata cacaacctac tgtcccgaac caactacttt tactaccaat     1320

ggtaaaacat acacagttac cgaaccaaca acattaacca ttactgactg tccatgcact     1380

attgagaagc caaccactac ctccacaacc gaatacactg ttgttactga atacactact     1440

tactgccccg aacctacaac atttactaca aacggcaaaa cttatacagt cacagagcca     1500

actactttaa ccattacaga ctgtccttgt actatcgaaa agcctacaac aacttctaca     1560

accgaatata ccgtcgtcac cgaatataca acttattgcc cagaacccac cactttcaca     1620

accaatggta agacttatac cgttactgag cctaccactt tgactattac tgattgccca     1680

tgtacaattg aaaaacctac tactacttct actactgagt atacagtcgt caccgagtat     1740

actacttatt gtcccgaacc tactactttc accactaatg gcaaaaccta tactgttacc     1800

gagcccacaa ctttaactat aactgattgc ccttgcacta ttgaaaaacc aacaactact     1860

tctaccactg agtatactgt cgttactgag tacaccacat attgtcccga acctacaacc     1920

tttactacta acggcaagac atacactgtt acagagccca ctactttaac tatcaccgat     1980

tgtccatgca ccatcgaaaa atctgaagct ccagagtctt ctgtcccagt tacagaatct     2040

aaaggtacta ctacaaagga aactggtgtt actactaaac aaactactgc taatccttct     2100

ttgacagttt ctacagttgt tccagtctct tcttctgctt cttctcattc tgttgttatt     2160

aattctaacg gtgcaaatgt cgttgttccc ggtgctttgg gtttggctgg agttgctatg     2220

ttgtttttg                                                             2229


<210>  32
<211>  1404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding adalimumab VH and an IgG1 Fc comprising the N297A 
       mutation (with 1-19 S. cerevisiae pre-pro alpha-mating factor 
       signal sequence)

<400>  32
agattcccat ccatcttcac tgctgttttg ttcgctgctt cttctgcttt ggctgaagtt       60

caattggttg aatctggtgg tggtttggtt cagcccggta ggtctttgag gttgtcttgc      120

gctgcatctg gtttcacttt cgacgactac gctatgcatt gggtcagaca agctcccggt      180

aagggtttgg aatgggtttc tgctattact tggaactctg gtcatattga ttatgctgat      240

tctgttgaag gtagatttac tatttctaga gataatgcta aaaactcttt gtacttgcag      300

atgaactctt tgagggctga ggacactgct gtctactact gtgctaaggt ttcttatttg      360

tctactgctt cttctttgga ctactggggt caaggtactt tggttactgt ctcgagtgct      420

tcaactaagg gaccatctgt cttcccattg gctccatctt caaagtctac ttcaggtggt      480

actgctgctt tgggttgctt ggtcaaagac tacttcccag agccagtcac agtttcttgg      540

aactctggtg ctttgacttc tggtgtccac actttcccag cagtcttaca atcttctggt      600

ttgtattcat tgtcttctgt tgttacagtt ccatcttctt ctttgggtac tcaaacttat      660

atttgtaatg ttaaccataa accatctaat actaaagttg ataagaaagt tgaaccaaaa      720

tcttgtgata agactcacac ttgtccacct tgcccagctc cagaattgtt aggtggtcct      780

tctgtcttct tgttcccacc aaagccaaaa gatacattga tgatttctag aactccagag      840

gttacatgtg tcgtcgtcga tgtctctcac gaggatcctg aggtcaagtt caactggtac      900

gtcgacggtg ttgaggtcca caacgctaag actaagccaa gagaagaaca atacgcttct      960

acatatagag ttgtctctgt tttgactgtt ttgcatcagg attggttaaa tggtaaagaa     1020

tataagtgta aagtttctaa taaggcttta ccagcaccaa ttgaaaaaac tatttctaag     1080

gctaagggtc aaccaaggga accacaggtc tacacattgc caccatcaag agaagaaatg     1140

actaaaaatc aagtttcttt aacttgcttg gttaagggtt tctatccatc agatattgct     1200

gtcgagtggg aatctaatgg tcagccagaa aataattata aaacaactcc accagttttg     1260

gattctgatg gttctttctt tttatattct aaattgacag tcgataagtc taggtggcag     1320

caaggtaacg ttttctcatg ctctgtcatg cacgaggctt tgcacaacca ctacacacaa     1380

aaatctttgt cattatctcc aggt                                            1404


<210>  33
<211>  699
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding adalimumab VL - Kappa (with 1-19 S. cerevisiae 
       pre-pro alpha-mating factor signal sequence)

<400>  33
atgagattcc catccatctt caccgccgtt ttgttcgctg cttcttccgc tttggctgat       60

attcaaatga ctcaatctcc atcttctttg tctgcttctg tcggtgatcg tgtcactatt      120

acttgtcgtg cttctcaagg tattagaaac tacttggctt ggtatcaaca gaagcccggt      180

aaggctccaa agttgttgat ttacgctgct tctactttgc agtctggtgt cccatctagg      240

ttctctggtt ctggttctgg tactgatttt actttgacta tttcttcttt gcaaccagaa      300

gatgttgcta cttattattg tcaaagatat aatagagctc catatacttt tggtcaaggt      360

accaaggttg agatcaagag aactgttgct gctccatccg ttttcatttt cccaccatcc      420

gacgaacagt tgaagtctgg tacagcttcc gttgtttgtt tgttgaacaa cttctaccca      480

agagaggcta aggttcagtg gaaggttgac aacgctttgc aatccggtaa ctcccaagaa      540

tccgttactg agcaagactc taaggactcc acttactcct tgtcctccac tttgactttg      600

tccaaggctg attacgagaa gcacaaggtt tacgcttgtg aggttacaca tcagggtttg      660

tcctccccag ttactaagtc cttcaacaga ggagagtgt                             699


<210>  34
<211>  680
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO5-680 without signal sequence

<400>  34

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala 
65                  70                  75                  80  


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn 
                85                  90                  95      


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
            100                 105                 110         


Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr Glu Pro Trp Thr Gly Thr 
        115                 120                 125             


Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Asp Thr Asn Gly 
    130                 135                 140                 


Gln Leu Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Thr Ala 
145                 150                 155                 160 


Ser Thr Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
                165                 170                 175     


Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro Thr 
            180                 185                 190         


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
        195                 200                 205             


Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
    210                 215                 220                 


Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr 
225                 230                 235                 240 


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Thr Thr Thr 
                245                 250                 255     


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Val Thr 
            260                 265                 270         


Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val 
        275                 280                 285             


Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Thr Thr Thr Thr Glu Pro 
    290                 295                 300                 


Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr 
305                 310                 315                 320 


Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr 
                325                 330                 335     


Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly 
            340                 345                 350         


Thr Phe Thr Ser Thr Ser Thr Glu Val Thr Thr Ile Thr Gly Thr Asn 
        355                 360                 365             


Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser 
    370                 375                 380                 


Glu Gly Leu Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr 
385                 390                 395                 400 


Ser Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro 
                405                 410                 415     


Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu 
            420                 425                 430         


Ile Thr Arg Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser 
        435                 440                 445             


Thr Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu 
    450                 455                 460                 


Thr Val Ile Val Ile Arg Thr Pro Thr Thr Ala Ile Ser Ser Ser Thr 
465                 470                 475                 480 


Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr 
                485                 490                 495     


Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr 
            500                 505                 510         


Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr 
        515                 520                 525             


Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile 
    530                 535                 540                 


Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr 
545                 550                 555                 560 


Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
                565                 570                 575     


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
            580                 585                 590         


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro 
        595                 600                 605             


Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu 
    610                 615                 620                 


Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val 
625                 630                 635                 640 


Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val 
                645                 650                 655     


Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu 
            660                 665                 670         


Ala Gly Val Ala Met Leu Phe Leu 
        675                 680 


<210>  35
<211>  660
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO1-660 without signal sequence

<400>  35

Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val Thr Ser Ser 
1               5                   10                  15      


Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr Ser Ser Glu 
            20                  25                  30          


Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro Thr Glu Thr 
        35                  40                  45              


Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr 
    50                  55                  60                  


Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser Thr Glu Ala 
65                  70                  75                  80  


Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu Pro Thr Asn 
                85                  90                  95      


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
            100                 105                 110         


Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr Glu Pro Trp Thr Gly Thr 
        115                 120                 125             


Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Gly Thr Asn Gly 
    130                 135                 140                 


Val Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu 
145                 150                 155                 160 


Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
                165                 170                 175     


Thr Ser Thr Glu Met Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr 
            180                 185                 190         


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
        195                 200                 205             


Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
    210                 215                 220                 


Glu Met Thr His Val Thr Gly Thr Asn Gly Val Pro Thr Asp Glu Thr 
225                 230                 235                 240 


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr 
                245                 250                 255     


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Val Thr 
            260                 265                 270         


Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val 
        275                 280                 285             


Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro 
    290                 295                 300                 


Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr 
305                 310                 315                 320 


Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr 
                325                 330                 335     


Pro Thr Ser Glu Gly Leu Val Thr Thr Thr Thr Glu Pro Trp Thr Gly 
            340                 345                 350         


Thr Phe Thr Ser Thr Ser Thr Glu Met Ser Thr Val Thr Gly Thr Asn 
        355                 360                 365             


Gly Leu Pro Thr Asp Glu Thr Val Ile Val Val Lys Thr Pro Thr Thr 
    370                 375                 380                 


Ala Ile Ser Ser Ser Leu Ser Ser Ser Ser Ser Gly Gln Ile Thr Ser 
385                 390                 395                 400 


Ser Ile Thr Ser Ser Arg Pro Ile Ile Thr Pro Phe Tyr Pro Ser Asn 
                405                 410                 415     


Gly Thr Ser Val Ile Ser Ser Ser Val Ile Ser Ser Ser Val Thr Ser 
            420                 425                 430         


Ser Leu Phe Thr Ser Ser Pro Val Ile Ser Ser Ser Val Ile Ser Ser 
        435                 440                 445             


Ser Thr Thr Thr Ser Thr Ser Ile Phe Ser Glu Thr Thr Ser Ala Phe 
    450                 455                 460                 


Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro 
465                 470                 475                 480 


Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu 
                485                 490                 495     


Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys 
            500                 505                 510         


Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro 
        515                 520                 525             


Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val 
    530                 535                 540                 


Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr 
545                 550                 555                 560 


Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr 
                565                 570                 575     


Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu Ser Ser Val 
            580                 585                 590         


Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr Gly Val Thr 
        595                 600                 605             


Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser Thr Val Val 
    610                 615                 620                 


Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile Asn Ser Asn 
625                 630                 635                 640 


Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala Gly Val Ala 
                645                 650                 655     


Met Leu Phe Leu 
            660 


<210>  36
<211>  2826
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding SED1-FLO5-680 fusion anchor (IgG1 (N297A) with 1-19 
       S. cerevisiae pre-pro alpha-mating factor signal sequence and 
       linker)

<400>  36
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgac       60

aagacacata cttgtccacc atgtccagct ccagaattgt tgggtggtcc atccgttttc      120

ttgttcccac caaagccaaa ggacactttg atgatctcca gaactccaga ggttacatgt      180

gttgttgttg acgtttctca cgaggaccca gaggttaagt tcaactggta cgttgacggt      240

gttgaagttc acaacgctaa gactaagcca agagaagagc agtacgcttc cacttacaga      300

gttgtttccg ttttgactgt tttgcaccag gactggttga acggtaaaga atacaagtgt      360

aaggtttcca acaaggcttt gccagctcca atcgaaaaga ctatctccaa ggctaagggt      420

caaccaagag agccacaggt ttacactttg ccaccatcca gagaagagat gactaagaac      480

caggtttcct tgacttgttt ggttaaagga ttctacccat ccgacattgc tgttgagtgg      540

gaatctaacg gtcaaccaga gaacaactac aagactactc caccagtttt ggattctgat      600

ggttccttct tcttgtactc caagttgact gttgacaagt ccagatggca acagggtaac      660

gttttctcct gttccgttat gcatgaggct ttgcacaacc actacactca aaagtccttg      720

tctttgtccc ctaaagctag cggaggtgga ggttctgaag ccgcggcgaa agaggcagct      780

gccaaacagt ttagcaatag tacgagcgcc tcctcaaccg atgtgacgtc atcaagctcc      840

attagtacaa gtagtggcag cgtcacaata actagtagcg aggcacccga gtcagacaat      900

ggcaccagta cagcagctcc taccgagact agtacagagg ccccgaccac agctatccca      960

accaatggca catccaccga agcccccact acggcgattc ccacgaacgg tacgtccacg     1020

gaagctccca cagacaccac tacagaggcg cctacgaccg ccctacctac taatgggacc     1080

agtacggaag ccccaacgga tactaccaca gaggcaccca cgacgggact accaacaaac     1140

ggcactacca cggagccgtg gaccggtacc tttacgagca cgtctaccga aatgactact     1200

atcacggaca ccaatggtca acttacggat gagacggtga ttgtgataag aactccaact     1260

accgcaagca ctatcaccac aaccacggaa ccttggactg gaactttcac atctacctcc     1320

accgagatga ctaccgtaac gggcacgaat ggacaaccga ctgacgaaac tgtaatagtc     1380

atccgtacgc ccacgtcaga gggcttaatt acaactacaa ccgagccgtg gacgggaaca     1440

ttcaccagta caagcacaga gatgaccacg gtaaccggca caaacgggca gcctacagat     1500

gagacagtaa tcgttatacg tacacccacc tccgaaggat tgataactac tacaaccgaa     1560

ccctggacgg gaaccttcac ctctacgagt actgaagtaa cgaccataac tgggacaaat     1620

ggccaaccta ctgatgaaac agtcatcgta atcaggaccc cgaccagcga gggtttgatt     1680

acgaccacca cggagccatg gacaggaact tttactagca caagtaccga aatgactact     1740

gtcactggaa ctaacggcca gccgactgac gagacggtaa ttgtaattag gacccccact     1800

agtgagggtc taataagtac aactactgaa ccttggacgg ggacgttcac atccacgagc     1860

acagaggtca ccacaattac agggaccaac ggacagccaa ctgatgagac tgtgatcgtt     1920

attaggaccc caacatctga agggttaatc accacgacca ccgagccgtg gacaggtaca     1980

tttacctcta ccagcacaga gatgacaact gtaacaggta ctaatggaca accgactgac     2040

gagacagtca tcgtcatccg aacccccacg agtgaaggct tgattacgcg aacaacggaa     2100

ccctggaccg gcaccttcac aagtacgtca acagaggtca ctacgattac gggaactaat     2160

gggcagccaa ctgatgagac agtcatcgta attcggacgc cgaccacggc catcagcagc     2220

agtacgacct ctgcattccc ccctactacc agcttgccac cgagcaatac tactactact     2280

cccccgtata accctagtac agattatacc acagattaca ctgtagttac cgagtacacc     2340

acatactgtc ccgaaccaac tacgtttacc accaatggga aaacgtacac cgtgaccgaa     2400

ccgacaactc tcactattac tgactgccca tgtactattg agaagcccac cacgacttca     2460

accactgaat ataccgtagt gactgaatat acaacctact gccctgaacc tacaacattc     2520

accactaatg gcaagactta caccgtgacg gaacctacaa cattgacaat taccgactgt     2580

ccttgcacta tcgagaaatc tgaggcccca gaaagttctg tcccagttac tgaatccaag     2640

gggacgacaa ctaaggagac tggggtaacg accaagcaga caactgccaa cccaagtctc     2700

accgtaagca ctgtggtacc ggtaagtagt tcagcgagta gtcactctgt tgtaatcaac     2760

tccaatggcg ccaacgttgt agtaccgggg gcacttggtc ttgctggggt tgcgatgctg     2820

ttcttg                                                                2826


<210>  37
<211>  942
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO5-680 fusion anchor (IgG1 (N297A) with 1-19 S. cerevisiae
       pre-pro alpha-mating factor signal sequence and linker)

<400>  37

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            20                  25                  30          


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        35                  40                  45              


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    50                  55                  60                  


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
65                  70                  75                  80  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            100                 105                 110         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        115                 120                 125             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    130                 135                 140                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
145                 150                 155                 160 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                165                 170                 175     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            180                 185                 190         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        195                 200                 205             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    210                 215                 220                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
225                 230                 235                 240 


Ser Leu Ser Pro Lys Ala Ser Gly Gly Gly Gly Ser Glu Ala Ala Ala 
                245                 250                 255     


Lys Glu Ala Ala Ala Lys Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser 
            260                 265                 270         


Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val 
        275                 280                 285             


Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr 
    290                 295                 300                 


Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro 
305                 310                 315                 320 


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn 
                325                 330                 335     


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
            340                 345                 350         


Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr 
        355                 360                 365             


Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr 
    370                 375                 380                 


Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr 
385                 390                 395                 400 


Ile Thr Asp Thr Asn Gly Gln Leu Thr Asp Glu Thr Val Ile Val Ile 
                405                 410                 415     


Arg Thr Pro Thr Thr Ala Ser Thr Ile Thr Thr Thr Thr Glu Pro Trp 
            420                 425                 430         


Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr Gly 
        435                 440                 445             


Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro 
    450                 455                 460                 


Thr Ser Glu Gly Leu Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr 
465                 470                 475                 480 


Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn Gly 
                485                 490                 495     


Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu 
            500                 505                 510         


Gly Leu Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
        515                 520                 525             


Thr Ser Thr Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr 
    530                 535                 540                 


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
545                 550                 555                 560 


Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
                565                 570                 575     


Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr 
            580                 585                 590         


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr 
        595                 600                 605             


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Val Thr 
    610                 615                 620                 


Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val 
625                 630                 635                 640 


Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Thr Thr Thr Thr Glu Pro 
                645                 650                 655     


Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr 
            660                 665                 670         


Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr 
        675                 680                 685             


Pro Thr Ser Glu Gly Leu Ile Thr Arg Thr Thr Glu Pro Trp Thr Gly 
    690                 695                 700                 


Thr Phe Thr Ser Thr Ser Thr Glu Val Thr Thr Ile Thr Gly Thr Asn 
705                 710                 715                 720 


Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Thr 
                725                 730                 735     


Ala Ile Ser Ser Ser Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu 
            740                 745                 750         


Pro Pro Ser Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp 
        755                 760                 765             


Tyr Thr Thr Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro 
    770                 775                 780                 


Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu 
785                 790                 795                 800 


Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro 
                805                 810                 815     


Thr Thr Thr Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr 
            820                 825                 830         


Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr 
        835                 840                 845             


Val Thr Glu Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile 
    850                 855                 860                 


Glu Lys Ser Glu Ala Pro Glu Ser Ser Val Pro Val Thr Glu Ser Lys 
865                 870                 875                 880 


Gly Thr Thr Thr Lys Glu Thr Gly Val Thr Thr Lys Gln Thr Thr Ala 
                885                 890                 895     


Asn Pro Ser Leu Thr Val Ser Thr Val Val Pro Val Ser Ser Ser Ala 
            900                 905                 910         


Ser Ser His Ser Val Val Ile Asn Ser Asn Gly Ala Asn Val Val Val 
        915                 920                 925             


Pro Gly Ala Leu Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
    930                 935                 940         


<210>  38
<211>  923
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO5-680 fusion anchor (IgG1 (N297A)

<400>  38

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Lys Ala Ser Gly Gly Gly Gly Ser Glu Ala Ala Ala Lys Glu Ala 
225                 230                 235                 240 


Ala Ala Lys Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val 
                245                 250                 255     


Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr 
            260                 265                 270         


Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro 
        275                 280                 285             


Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly 
    290                 295                 300                 


Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser 
305                 310                 315                 320 


Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu 
                325                 330                 335     


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu 
            340                 345                 350         


Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr Glu Pro Trp 
        355                 360                 365             


Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Asp 
    370                 375                 380                 


Thr Asn Gly Gln Leu Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro 
385                 390                 395                 400 


Thr Thr Ala Ser Thr Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr 
                405                 410                 415     


Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn Gly 
            420                 425                 430         


Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu 
        435                 440                 445             


Gly Leu Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
    450                 455                 460                 


Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro Thr 
465                 470                 475                 480 


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
                485                 490                 495     


Thr Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
            500                 505                 510         


Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr 
        515                 520                 525             


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Thr Thr Thr 
    530                 535                 540                 


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr 
545                 550                 555                 560 


Thr Val Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val 
                565                 570                 575     


Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro 
            580                 585                 590         


Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Val Thr Thr Ile Thr 
        595                 600                 605             


Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr 
    610                 615                 620                 


Pro Thr Ser Glu Gly Leu Ile Thr Thr Thr Thr Glu Pro Trp Thr Gly 
625                 630                 635                 640 


Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Val Thr Gly Thr Asn 
                645                 650                 655     


Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser 
            660                 665                 670         


Glu Gly Leu Ile Thr Arg Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr 
        675                 680                 685             


Ser Thr Ser Thr Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro 
    690                 695                 700                 


Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Thr Ala Ile Ser 
705                 710                 715                 720 


Ser Ser Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser 
                725                 730                 735     


Asn Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr 
            740                 745                 750         


Asp Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr 
        755                 760                 765             


Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr 
    770                 775                 780                 


Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr 
785                 790                 795                 800 


Ser Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro 
                805                 810                 815     


Glu Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu 
            820                 825                 830         


Pro Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser 
        835                 840                 845             


Glu Ala Pro Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr 
    850                 855                 860                 


Thr Lys Glu Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser 
865                 870                 875                 880 


Leu Thr Val Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His 
                885                 890                 895     


Ser Val Val Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala 
            900                 905                 910         


Leu Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
        915                 920             


<210>  39
<211>  2766
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding SED1-FLO1-660 fusion anchor (with 1-19 S. cerevisiae
       pre-pro alpha-mating factor signal sequence)

<400>  39
atgagatttc cttcaatttt tactgctgtt ttattcgcag catcctccgc attagctgac       60

aagacacata cttgtccacc atgtccagct ccagaattgt tgggtggtcc atccgttttc      120

ttgttcccac caaagccaaa ggacactttg atgatctcca gaactccaga ggttacatgt      180

gttgttgttg acgtttctca cgaggaccca gaggttaagt tcaactggta cgttgacggt      240

gttgaagttc acaacgctaa gactaagcca agagaagagc agtacgcttc cacttacaga      300

gttgtttccg ttttgactgt tttgcaccag gactggttga acggtaaaga atacaagtgt      360

aaggtttcca acaaggcttt gccagctcca atcgaaaaga ctatctccaa ggctaagggt      420

caaccaagag agccacaggt ttacactttg ccaccatcca gagaagagat gactaagaac      480

caggtttcct tgacttgttt ggttaaagga ttctacccat ccgacattgc tgttgagtgg      540

gaatctaacg gtcaaccaga gaacaactac aagactactc caccagtttt ggattctgat      600

ggttccttct tcttgtactc caagttgact gttgacaagt ccagatggca acagggtaac      660

gttttctcct gttccgttat gcatgaggct ttgcacaacc actacactca aaagtccttg      720

tctttgtccc ctaaagctag cggaggaggt ggaagcgaag ctgccgctaa ggaggcagcc      780

gccaagcagt tttctaattc cacgagcgcg tctagtaccg acgtgacatc tagttcatcc      840

atcagtacta gcagcggctc cgtcactata actagctctg aggcacccga gagcgacaat      900

ggtacatcaa cagctgcccc tacagaaact tctacggagg cccccacgac cgccatacct      960

accaatggca ccagtactga ggcgcctact accgcaatcc cgacaaacgg aaccagcaca     1020

gaagctccaa ctgatacaac aacagaagcc ccaacaacag cactgccgac gaacggcacg     1080

tctaccgaag ctcccacgga cacgacaact gaggcgccga ctacaggatt acccacaaat     1140

gggactacca ccgagccttg gacagggacc ttcacctcta cgtctaccga aatgacaaca     1200

ataaccggta cgaacggagt accaactgac gagactgtca ttgttattcg tacaccaaca     1260

agcgaaggtc ttataagcac gacgactgag ccatggactg ggacttttac gagtacgagt     1320

acggagatga ctactataac aggcaccaat ggccagccca ctgacgagac cgtcatagtt     1380

ataaggactc ctacctcaga aggattaatc tctacaacaa cagagccatg gacgggcacc     1440

ttcacttcaa cgtcaactga gatgacacat gtgacaggta cgaatggtgt accaaccgac     1500

gagacggtca tcgtgattag gactcccaca agcgaggggc tgatatcaac aactactgaa     1560

ccctggacgg gtacgttcac tagtacatcc accgaagtta cgactataac cggaacgaac     1620

gggcaaccca ctgatgaaac cgttattgtg atcaggacgc ctacttccga aggtctgata     1680

tctaccacga ctgagccctg gacgggcacg ttcacttcaa cttccactga aatgacgacg     1740

gtgactggaa ccaacggtca accgaccgac gaaaccgtca tagtgattag gacacctacc     1800

agtgagggac tagttaccac taccacggaa ccatggactg gtacatttac atcaacatcc     1860

accgagatgt ctacagttac gggcacaaat ggcttgccta cagatgaaac cgtgatagtt     1920

gttaaaacgc ccaccactgc aatttcaagc agcttgtcta gctctagctc cggacaaatt     1980

accagttcca taacatctag ccgtccaatt ataacaccat tttatccaag caacggcact     2040

agtgtgattt cctccagtgt tatatcatct agtgttacgt catcactatt cacatcatcc     2100

cctgtcatct cttcatccgt tatctcctct tctacgacca cgtcaacgtc aatttttagt     2160

gaaacaacct ctgcatttcc cccgacgacg tctctacctc caagtaatac gactactact     2220

cccccttata atccgagcac agactacaca actgactata ccgtcgttac ggagtacacg     2280

acgtattgcc ctgaacccac aacctttact actaatggga aaacttacac tgtcactgaa     2340

ccgaccacgc taacgattac agactgcccc tgcacgatag aaaaacctac caccacttca     2400

accacggagt acactgtggt gactgaatac acgacatact gtccagaacc tacaaccttc     2460

acaaccaacg gaaaaacata cacggtgacg gaacctacaa cgctgacgat cactgattgc     2520

ccatgtacaa tcgagaaatc tgaagctcca gaatcatctg tcccggttac tgaatcaaag     2580

ggtactacaa ccaaggaaac tggggtcact acaaaacaaa ccacggcaaa tccgagtttg     2640

acagtcagta cggtagtgcc tgtgtctagt agcgccagtt cacacagcgt agtgataaat     2700

tctaatgggg cgaacgtggt tgtacctggc gcgttaggct tggccggggt agccatgctt     2760

tttttg                                                                2766


<210>  40
<211>  922
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO1-660 fusion anchor (with 1-19 S. cerevisiae pre-pro 
       alpha-mating factor signal sequence)

<400>  40

Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      


Ala Leu Ala Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
            20                  25                  30          


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        35                  40                  45              


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    50                  55                  60                  


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
65                  70                  75                  80  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala 
                85                  90                  95      


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
            100                 105                 110         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
        115                 120                 125             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
    130                 135                 140                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
145                 150                 155                 160 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                165                 170                 175     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            180                 185                 190         


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        195                 200                 205             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    210                 215                 220                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
225                 230                 235                 240 


Ser Leu Ser Pro Lys Ala Ser Gly Gly Gly Gly Ser Glu Ala Ala Ala 
                245                 250                 255     


Lys Glu Ala Ala Ala Lys Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser 
            260                 265                 270         


Thr Asp Val Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val 
        275                 280                 285             


Thr Ile Thr Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr 
    290                 295                 300                 


Ala Ala Pro Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro 
305                 310                 315                 320 


Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn 
                325                 330                 335     


Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr 
            340                 345                 350         


Thr Ala Leu Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr 
        355                 360                 365             


Thr Thr Glu Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr 
    370                 375                 380                 


Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr 
385                 390                 395                 400 


Ile Thr Gly Thr Asn Gly Val Pro Thr Asp Glu Thr Val Ile Val Ile 
                405                 410                 415     


Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp 
            420                 425                 430         


Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Gly 
        435                 440                 445             


Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro 
    450                 455                 460                 


Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr 
465                 470                 475                 480 


Phe Thr Ser Thr Ser Thr Glu Met Thr His Val Thr Gly Thr Asn Gly 
                485                 490                 495     


Val Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu 
            500                 505                 510         


Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
        515                 520                 525             


Thr Ser Thr Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr 
    530                 535                 540                 


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
545                 550                 555                 560 


Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
                565                 570                 575     


Glu Met Thr Thr Val Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr 
            580                 585                 590         


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Val Thr Thr Thr 
        595                 600                 605             


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Ser 
    610                 615                 620                 


Thr Val Thr Gly Thr Asn Gly Leu Pro Thr Asp Glu Thr Val Ile Val 
625                 630                 635                 640 


Val Lys Thr Pro Thr Thr Ala Ile Ser Ser Ser Leu Ser Ser Ser Ser 
                645                 650                 655     


Ser Gly Gln Ile Thr Ser Ser Ile Thr Ser Ser Arg Pro Ile Ile Thr 
            660                 665                 670         


Pro Phe Tyr Pro Ser Asn Gly Thr Ser Val Ile Ser Ser Ser Val Ile 
        675                 680                 685             


Ser Ser Ser Val Thr Ser Ser Leu Phe Thr Ser Ser Pro Val Ile Ser 
    690                 695                 700                 


Ser Ser Val Ile Ser Ser Ser Thr Thr Thr Ser Thr Ser Ile Phe Ser 
705                 710                 715                 720 


Glu Thr Thr Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn 
                725                 730                 735     


Thr Thr Thr Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp 
            740                 745                 750         


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
        755                 760                 765             


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
    770                 775                 780                 


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser 
785                 790                 795                 800 


Thr Thr Glu Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu 
                805                 810                 815     


Pro Thr Thr Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro 
            820                 825                 830         


Thr Thr Leu Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu 
        835                 840                 845             


Ala Pro Glu Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr 
    850                 855                 860                 


Lys Glu Thr Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu 
865                 870                 875                 880 


Thr Val Ser Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser 
                885                 890                 895     


Val Val Ile Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu 
            900                 905                 910         


Gly Leu Ala Gly Val Ala Met Leu Phe Leu 
        915                 920         


<210>  41
<211>  903
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SED1-FLO1-660 fusion anchor

<400>  41

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Lys Ala Ser Gly Gly Gly Gly Ser Glu Ala Ala Ala Lys Glu Ala 
225                 230                 235                 240 


Ala Ala Lys Gln Phe Ser Asn Ser Thr Ser Ala Ser Ser Thr Asp Val 
                245                 250                 255     


Thr Ser Ser Ser Ser Ile Ser Thr Ser Ser Gly Ser Val Thr Ile Thr 
            260                 265                 270         


Ser Ser Glu Ala Pro Glu Ser Asp Asn Gly Thr Ser Thr Ala Ala Pro 
        275                 280                 285             


Thr Glu Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly 
    290                 295                 300                 


Thr Ser Thr Glu Ala Pro Thr Thr Ala Ile Pro Thr Asn Gly Thr Ser 
305                 310                 315                 320 


Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu Ala Pro Thr Thr Ala Leu 
                325                 330                 335     


Pro Thr Asn Gly Thr Ser Thr Glu Ala Pro Thr Asp Thr Thr Thr Glu 
            340                 345                 350         


Ala Pro Thr Thr Gly Leu Pro Thr Asn Gly Thr Thr Thr Glu Pro Trp 
        355                 360                 365             


Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Gly 
    370                 375                 380                 


Thr Asn Gly Val Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro 
385                 390                 395                 400 


Thr Ser Glu Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr 
                405                 410                 415     


Phe Thr Ser Thr Ser Thr Glu Met Thr Thr Ile Thr Gly Thr Asn Gly 
            420                 425                 430         


Gln Pro Thr Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu 
        435                 440                 445             


Gly Leu Ile Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser 
    450                 455                 460                 


Thr Ser Thr Glu Met Thr His Val Thr Gly Thr Asn Gly Val Pro Thr 
465                 470                 475                 480 


Asp Glu Thr Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile 
                485                 490                 495     


Ser Thr Thr Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr 
            500                 505                 510         


Glu Val Thr Thr Ile Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr 
        515                 520                 525             


Val Ile Val Ile Arg Thr Pro Thr Ser Glu Gly Leu Ile Ser Thr Thr 
    530                 535                 540                 


Thr Glu Pro Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Thr 
545                 550                 555                 560 


Thr Val Thr Gly Thr Asn Gly Gln Pro Thr Asp Glu Thr Val Ile Val 
                565                 570                 575     


Ile Arg Thr Pro Thr Ser Glu Gly Leu Val Thr Thr Thr Thr Glu Pro 
            580                 585                 590         


Trp Thr Gly Thr Phe Thr Ser Thr Ser Thr Glu Met Ser Thr Val Thr 
        595                 600                 605             


Gly Thr Asn Gly Leu Pro Thr Asp Glu Thr Val Ile Val Val Lys Thr 
    610                 615                 620                 


Pro Thr Thr Ala Ile Ser Ser Ser Leu Ser Ser Ser Ser Ser Gly Gln 
625                 630                 635                 640 


Ile Thr Ser Ser Ile Thr Ser Ser Arg Pro Ile Ile Thr Pro Phe Tyr 
                645                 650                 655     


Pro Ser Asn Gly Thr Ser Val Ile Ser Ser Ser Val Ile Ser Ser Ser 
            660                 665                 670         


Val Thr Ser Ser Leu Phe Thr Ser Ser Pro Val Ile Ser Ser Ser Val 
        675                 680                 685             


Ile Ser Ser Ser Thr Thr Thr Ser Thr Ser Ile Phe Ser Glu Thr Thr 
    690                 695                 700                 


Ser Ala Phe Pro Pro Thr Thr Ser Leu Pro Pro Ser Asn Thr Thr Thr 
705                 710                 715                 720 


Thr Pro Pro Tyr Asn Pro Ser Thr Asp Tyr Thr Thr Asp Tyr Thr Val 
                725                 730                 735     


Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr Phe Thr Thr 
            740                 745                 750         


Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu Thr Ile Thr 
        755                 760                 765             


Asp Cys Pro Cys Thr Ile Glu Lys Pro Thr Thr Thr Ser Thr Thr Glu 
    770                 775                 780                 


Tyr Thr Val Val Thr Glu Tyr Thr Thr Tyr Cys Pro Glu Pro Thr Thr 
785                 790                 795                 800 


Phe Thr Thr Asn Gly Lys Thr Tyr Thr Val Thr Glu Pro Thr Thr Leu 
                805                 810                 815     


Thr Ile Thr Asp Cys Pro Cys Thr Ile Glu Lys Ser Glu Ala Pro Glu 
            820                 825                 830         


Ser Ser Val Pro Val Thr Glu Ser Lys Gly Thr Thr Thr Lys Glu Thr 
        835                 840                 845             


Gly Val Thr Thr Lys Gln Thr Thr Ala Asn Pro Ser Leu Thr Val Ser 
    850                 855                 860                 


Thr Val Val Pro Val Ser Ser Ser Ala Ser Ser His Ser Val Val Ile 
865                 870                 875                 880 


Asn Ser Asn Gly Ala Asn Val Val Val Pro Gly Ala Leu Gly Leu Ala 
                885                 890                 895     


Gly Val Ala Met Leu Phe Leu 
            900             


<210>  42
<211>  522
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S. cerevisiae PDI1 (amino acids 1-28 signal sequence)

<400>  42

Met Lys Phe Ser Ala Gly Ala Val Leu Ser Trp Ser Ser Leu Leu Leu 
1               5                   10                  15      


Ala Ser Ser Val Phe Ala Gln Gln Glu Ala Val Ala Pro Glu Asp Ser 
            20                  25                  30          


Ala Val Val Lys Leu Ala Thr Asp Ser Phe Asn Glu Tyr Ile Gln Ser 
        35                  40                  45              


His Asp Leu Val Leu Ala Glu Phe Phe Ala Pro Trp Cys Gly His Cys 
    50                  55                  60                  


Lys Asn Met Ala Pro Glu Tyr Val Lys Ala Ala Glu Thr Leu Val Glu 
65                  70                  75                  80  


Lys Asn Ile Thr Leu Ala Gln Ile Asp Cys Thr Glu Asn Gln Asp Leu 
                85                  90                  95      


Cys Met Glu His Asn Ile Pro Gly Phe Pro Ser Leu Lys Ile Phe Lys 
            100                 105                 110         


Asn Ser Asp Val Asn Asn Ser Ile Asp Tyr Glu Gly Pro Arg Thr Ala 
        115                 120                 125             


Glu Ala Ile Val Gln Phe Met Ile Lys Gln Ser Gln Pro Ala Val Ala 
    130                 135                 140                 


Val Val Ala Asp Leu Pro Ala Tyr Leu Ala Asn Glu Thr Phe Val Thr 
145                 150                 155                 160 


Pro Val Ile Val Gln Ser Gly Lys Ile Asp Ala Asp Phe Asn Ala Thr 
                165                 170                 175     


Phe Tyr Ser Met Ala Asn Lys His Phe Asn Asp Tyr Asp Phe Val Ser 
            180                 185                 190         


Ala Glu Asn Ala Asp Asp Asp Phe Lys Leu Ser Ile Tyr Leu Pro Ser 
        195                 200                 205             


Ala Met Asp Glu Pro Val Val Tyr Asn Gly Lys Lys Ala Asp Ile Ala 
    210                 215                 220                 


Asp Ala Asp Val Phe Glu Lys Trp Leu Gln Val Glu Ala Leu Pro Tyr 
225                 230                 235                 240 


Phe Gly Glu Ile Asp Gly Ser Val Phe Ala Gln Tyr Val Glu Ser Gly 
                245                 250                 255     


Leu Pro Leu Gly Tyr Leu Phe Tyr Asn Asp Glu Glu Glu Leu Glu Glu 
            260                 265                 270         


Tyr Lys Pro Leu Phe Thr Glu Leu Ala Lys Lys Asn Arg Gly Leu Met 
        275                 280                 285             


Asn Phe Val Ser Ile Asp Ala Arg Lys Phe Gly Arg His Ala Gly Asn 
    290                 295                 300                 


Leu Asn Met Lys Glu Gln Phe Pro Leu Phe Ala Ile His Asp Met Thr 
305                 310                 315                 320 


Glu Asp Leu Lys Tyr Gly Leu Pro Gln Leu Ser Glu Glu Ala Phe Asp 
                325                 330                 335     


Glu Leu Ser Asp Lys Ile Val Leu Glu Ser Lys Ala Ile Glu Ser Leu 
            340                 345                 350         


Val Lys Asp Phe Leu Lys Gly Asp Ala Ser Pro Ile Val Lys Ser Gln 
        355                 360                 365             


Glu Ile Phe Glu Asn Gln Asp Ser Ser Val Phe Gln Leu Val Gly Lys 
    370                 375                 380                 


Asn His Asp Glu Ile Val Asn Asp Pro Lys Lys Asp Val Leu Val Leu 
385                 390                 395                 400 


Tyr Tyr Ala Pro Trp Cys Gly His Cys Lys Arg Leu Ala Pro Thr Tyr 
                405                 410                 415     


Gln Glu Leu Ala Asp Thr Tyr Ala Asn Ala Thr Ser Asp Val Leu Ile 
            420                 425                 430         


Ala Lys Leu Asp His Thr Glu Asn Asp Val Arg Gly Val Val Ile Glu 
        435                 440                 445             


Gly Tyr Pro Thr Ile Val Leu Tyr Pro Gly Gly Lys Lys Ser Glu Ser 
    450                 455                 460                 


Val Val Tyr Gln Gly Ser Arg Ser Leu Asp Ser Leu Phe Asp Phe Ile 
465                 470                 475                 480 


Lys Glu Asn Gly His Phe Asp Val Asp Gly Lys Ala Leu Tyr Glu Glu 
                485                 490                 495     


Ala Gln Glu Lys Ala Ala Glu Glu Ala Asp Ala Asp Ala Glu Leu Ala 
            500                 505                 510         


Asp Glu Glu Asp Ala Ile His Asp Glu Leu 
        515                 520         


