                         SEQUENCE LISTING

<110>  Vivaldi Biosciences Inc.
 
<120>  INFLUENZA VIRUS ENCODING A TRUNCATED NS1 PROTEIN AND A SARS-COV 
       RECEPTOR BINDING DOMAIN

<130>  REDL-1133PCT

<160>  57    

<170>  PatentIn version 3.5

<210>  1
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Wuhan-Hu-1/2019 Reference strain (GenBank MN908947)

<400>  1

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  2
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCoV-19/Austria/CeMM0639/2020 (EPI_ISL_475936)

<400>  2

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  3
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCoV-19/Sweden/20-51988/2020 (EPI_ISL_4755690)

<400>  3

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  4
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCoV-19/Singapore/397/2020 (EPI_ISL_469146)

<400>  4

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  5
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCoV-19/USA/IL-NM0118/2020 (EPI_ISL_444601)

<400>  5

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val 
        


<210>  6
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS CoV-2 RBD amino acid sequence inserted (RBD219, see scheme 
       in Fig.1)

<400>  6

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  7
<211>  1325
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BGHB delNS-RBD219 BSMBI spm1

<400>  7
cgtctcaggg agcagaagca gaggatttgt ttagtcactg gcaaacagga aaaatggcgg       60

acaatatgac cacaacacaa attgaggtgg gtccgggagc aggatccgga gcaacaaatt      120

tctcacttct taaacaagca ggggacgtgg aagaaaatcc aggaccaatg aaaacagata      180

cacttcttct ttgggtgctg cttttgtggg ttccaagatc acatgggaat atcacgaatc      240

tgtgcccctt tggagaggtg ttcaatgcta cccgctttgc ttcagtgtac gcttggaata      300

ggaaacggat cagtaattgc gtggctgatt attctgtgct gtacaatagc gcaagcttca      360

gcacattcaa atgctatgga gtgagcccca caaaactgaa tgatctgtgc ttcactaatg      420

tttacgccga ttcatttgtg atacgcggag atgaggtgag acagattgcc cctggccaaa      480

caggaaaaat cgcggattac aattacaaac tgccagatga tttcactgga tgcgtgattg      540

catggaattc aaataatctg gatagtaaag ttggaggcaa ttacaattac ctgtacagac      600

tgttcagaaa aagcaatctg aaaccctttg agcgagatat cagcaccgaa atctaccagg      660

ctggctctac gccttgcaat ggagtggagg gattcaattg ttattttcct ctgcagagtt      720

acggattcca accgaccaat ggtgtgggat atcagcccta cagagtggtg gtgctgagct      780

tcgaactgct tcacgctcca gcaacagtgt gcggacccaa aaaatctact aatctggtga      840

aaaataagtg cgtgaatttc aatttcaatg gactgacggg aacgtaatga tgagcggccg      900

cccaagcaga aagtggtact aaccttcttc tctttcttct cctgacagtg gaggatgaag      960

aagatggcca tcggatcctc aattcactct tcgagcgtct taatgaagga cattcaaagc     1020

caattcgagc agctgaaact gcggtgggag tcttatccca atttggtcaa gagcaccgat     1080

tatcaccaga agagggagac aattagactg gtcacggaag aactttatct tttaagtaaa     1140

agaattgatg ataacatatt gttccacaaa acagtaatag ctaacagctc cataatagct     1200

gacatggttg tatcattatc attattagaa acattgtatg aaatgaagga tgtggttgaa     1260

gtgtacagca ggcagtgctt gtgaatttaa aataaaaatc ctcttgttac tactaatacg     1320

agacg                                                                 1325


<210>  8
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A Sequence (all in frame)

<400>  8

Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      


Pro Gly Pro 
            


<210>  9
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptides


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (4)..(4)
<223>  Xaa can be any naturally occurring amino acid

<400>  9

Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               


<210>  10
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  10
caggtagatt g                                                            11


<210>  11
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  11
caggtaagta t                                                            11


<210>  12
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  12
caggtaaatt g                                                            11


<210>  13
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  13
gaggtgggtc c                                                            11


<210>  14
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  14
gaggtaggtc c                                                            11


<210>  15
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  linker

<400>  15
tactaacctt cttctctttc ttctcctgac ag                                     32


<210>  16
<211>  1097
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Influenza B/Thueringen/02/06 wild-type NS segment nucleotide 
       sequence

<400>  16
agcagaagca gaggatttgt ttagtcactg gcaaacagga aaaatggcgg acaatatgac       60

cacaacacaa attgaggtgg gtccgggagc aaccaatgcc accataaact ttgaagcagg      120

aattctggag tgctatgaaa gactttcatg gcaaagggcc cttgactacc ctggtcaaga      180

ccgcctaaac agactaaaga gaaaattaga gtcaagaata aagactcaca acaaaagtga      240

gcctgaaagt aaaaggatgt ctcttgaaga gagaaaagca attggagtaa aaatgatgaa      300

agtactccta tttatgaatc cgtctgctgg aattgaaggg tttgagccat actgtatgaa      360

aagttcctca aagagcaact gtccgaaata caattggatc gattaccctt caaccccagg      420

gaggtgcctt gatgacatag aagaagaacc agatgatgtt gatggcccaa ctgaaatagt      480

attaagggac atgaacaaca aagatgcaag gcaaaagata aaggaggaag taaacactca      540

gaaagaaggg aagttccgtt tgacaataaa aagggatatg cgtaatgtat tgtccctgag      600

agtgttagta aacggaacat tcctcaaaca ccccaatggg tacaagtcct tatcaactct      660

gcatagattg aatgcatatg accagagtgg aaggcttgtt gctaaacttg ttgctactga      720

tgatcttaca gtggaggatg aagaagatgg ccatcggatc ctcaattcac tcttcgagcg      780

tcttaatgaa ggacattcaa agccaattcg agcagctgaa actgcggtgg gagtcttatc      840

ccaatttggt caagagcacc gattatcacc agaagaggga gacaattaga ctggtcacgg      900

aagaacttta tcttttaagt aaaagaattg atgataacat attgttccac aaaacagtaa      960

tagctaacag ctccataata gctgacatgg ttgtatcatt atcattatta gaaacattgt     1020

atgaaatgaa ggatgtggtt gaagtgtaca gcaggcagtg cttgtgaatt taaaataaaa     1080

atcctcttgt tactact                                                    1097


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  17

Gly Gly Ser Gly Gly 
1               5   


<210>  18
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  18

Gly Ser Gly Ser Gly 
1               5   


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  19

Gly Ser Gly Ser Gly Ser Gly Ser 
1               5               


<210>  20
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza B Thueringen/02/06 wild-type NS1 amino acid sequence

<400>  20

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Val Gly Pro Gly Ala 
1               5                   10                  15      


Thr Asn Ala Thr Ile Asn Phe Glu Ala Gly Ile Leu Glu Cys Tyr Glu 
            20                  25                  30          


Arg Leu Ser Trp Gln Arg Ala Leu Asp Tyr Pro Gly Gln Asp Arg Leu 
        35                  40                  45              


Asn Arg Leu Lys Arg Lys Leu Glu Ser Arg Ile Lys Thr His Asn Lys 
    50                  55                  60                  


Ser Glu Pro Glu Ser Lys Arg Met Ser Leu Glu Glu Arg Lys Ala Ile 
65                  70                  75                  80  


Gly Val Lys Met Met Lys Val Leu Leu Phe Met Asn Pro Ser Ala Gly 
                85                  90                  95      


Ile Glu Gly Phe Glu Pro Tyr Cys Met Lys Ser Ser Ser Lys Ser Asn 
            100                 105                 110         


Cys Pro Lys Tyr Asn Trp Ile Asp Tyr Pro Ser Thr Pro Gly Arg Cys 
        115                 120                 125             


Leu Asp Asp Ile Glu Glu Glu Pro Asp Asp Val Asp Gly Pro Thr Glu 
    130                 135                 140                 


Ile Val Leu Arg Asp Met Asn Asn Lys Asp Ala Arg Gln Lys Ile Lys 
145                 150                 155                 160 


Glu Glu Val Asn Thr Gln Lys Glu Gly Lys Phe Arg Leu Thr Ile Lys 
                165                 170                 175     


Arg Asp Met Arg Asn Val Leu Ser Leu Arg Val Leu Val Asn Gly Thr 
            180                 185                 190         


Phe Leu Lys His Pro Asn Gly Tyr Lys Ser Leu Ser Thr Leu His Arg 
        195                 200                 205             


Leu Asn Ala Tyr Asp Gln Ser Gly Arg Leu Val Ala Lys Leu Val Ala 
    210                 215                 220                 


Thr Asp Asp Leu Thr Val Glu Asp Glu Glu Asp Gly His Arg Ile Leu 
225                 230                 235                 240 


Asn Ser Leu Phe Glu Arg Leu Asn Glu Gly His Ser Lys Pro Ile Arg 
                245                 250                 255     


Ala Ala Glu Thr Ala Val Gly Val Leu Ser Gln Phe Gly Gln Glu His 
            260                 265                 270         


Arg Leu Ser Pro Glu Glu Gly Asp Asn 
        275                 280     


<210>  21
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  21

Gly Gly Gly Gly 
1               


<210>  22
<211>  752
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Thueringen/02/06 amino acid sequence PB1 D67N

<400>  22

Met Asn Ile Asn Pro Tyr Phe Leu Phe Ile Asp Val Pro Ile Gln Ala 
1               5                   10                  15      


Ala Ile Ser Thr Thr Phe Pro Tyr Thr Gly Val Pro Pro Tyr Ser His 
            20                  25                  30          


Gly Thr Gly Thr Gly Tyr Thr Ile Asp Thr Val Ile Arg Thr His Glu 
        35                  40                  45              


Tyr Ser Asn Lys Gly Lys Gln Tyr Val Ser Asp Ile Thr Gly Cys Thr 
    50                  55                  60                  


Met Ile Asn Pro Thr Asn Gly Pro Leu Pro Glu Asp Asn Glu Pro Ser 
65                  70                  75                  80  


Ala Tyr Ala Gln Leu Asp Cys Val Leu Glu Ala Leu Asp Arg Met Asp 
                85                  90                  95      


Glu Glu His Pro Gly Leu Phe Gln Ala Ala Ser Gln Asn Ala Met Glu 
            100                 105                 110         


Ala Leu Met Val Thr Thr Val Asp Lys Leu Thr Gln Gly Arg Gln Thr 
        115                 120                 125             


Phe Asp Trp Thr Val Cys Arg Asn Gln Pro Ala Ala Thr Ala Leu Asn 
    130                 135                 140                 


Thr Thr Ile Thr Ser Phe Arg Leu Asn Asp Leu Asn Gly Ala Asp Lys 
145                 150                 155                 160 


Gly Gly Leu Val Pro Phe Cys Gln Asp Ile Ile Asp Ser Leu Asp Lys 
                165                 170                 175     


Pro Glu Met Thr Phe Phe Ser Val Lys Asn Ile Lys Lys Lys Leu Pro 
            180                 185                 190         


Ala Lys Asn Arg Lys Gly Phe Leu Ile Lys Arg Ile Pro Met Lys Val 
        195                 200                 205             


Lys Asp Arg Ile Ser Arg Val Glu Tyr Ile Lys Arg Ala Leu Ser Leu 
    210                 215                 220                 


Asn Thr Met Thr Lys Asp Ala Glu Arg Gly Lys Leu Lys Arg Arg Ala 
225                 230                 235                 240 


Ile Ala Thr Ala Gly Ile Gln Ile Arg Gly Phe Val Leu Val Val Glu 
                245                 250                 255     


Asn Leu Ala Lys Asn Ile Cys Glu Asn Leu Glu Gln Ser Gly Leu Pro 
            260                 265                 270         


Val Gly Gly Asn Glu Lys Lys Ala Lys Leu Ser Asn Ala Val Ala Lys 
        275                 280                 285             


Met Leu Ser Asn Cys Pro Pro Gly Gly Ile Ser Met Thr Val Thr Gly 
    290                 295                 300                 


Asp Asn Thr Lys Trp Asn Glu Cys Leu Asn Pro Arg Ile Phe Leu Ala 
305                 310                 315                 320 


Met Thr Glu Arg Ile Thr Arg Asp Ser Pro Ile Trp Phe Arg Asp Phe 
                325                 330                 335     


Cys Ser Ile Ala Pro Val Leu Phe Ser Asn Lys Ile Ala Arg Leu Gly 
            340                 345                 350         


Lys Gly Phe Met Ile Thr Ser Lys Thr Lys Arg Leu Lys Ala Gln Ile 
        355                 360                 365             


Pro Cys Pro Asp Leu Phe Ser Ile Pro Leu Glu Arg Tyr Asn Glu Glu 
    370                 375                 380                 


Thr Arg Ala Lys Leu Lys Arg Leu Lys Pro Phe Phe Asn Glu Glu Gly 
385                 390                 395                 400 


Thr Ala Ser Leu Ser Pro Gly Met Met Met Gly Met Phe Asn Met Leu 
                405                 410                 415     


Ser Thr Val Leu Gly Val Ala Ala Leu Gly Ile Lys Asn Ile Gly Asn 
            420                 425                 430         


Lys Glu Tyr Leu Trp Asp Gly Leu Gln Ser Ser Asp Asp Phe Ala Leu 
        435                 440                 445             


Phe Val Asn Ala Lys Asp Glu Glu Thr Cys Met Glu Gly Ile Asn Asp 
    450                 455                 460                 


Phe Tyr Arg Thr Cys Lys Leu Leu Gly Ile Asn Met Ser Lys Lys Lys 
465                 470                 475                 480 


Ser Tyr Cys Asn Glu Thr Gly Met Phe Glu Phe Thr Ser Met Phe Tyr 
                485                 490                 495     


Arg Asp Gly Phe Val Ser Asn Phe Ala Met Glu Ile Pro Ser Phe Gly 
            500                 505                 510         


Val Ala Gly Val Asn Glu Ser Ala Asp Met Ala Ile Gly Met Thr Ile 
        515                 520                 525             


Ile Lys Asn Asn Met Ile Asn Asn Gly Met Gly Pro Ala Thr Ala Gln 
    530                 535                 540                 


Thr Ala Ile Gln Leu Phe Ile Ala Asp Tyr Arg Tyr Thr Tyr Lys Cys 
545                 550                 555                 560 


His Arg Gly Asp Ser Lys Val Glu Gly Lys Arg Met Lys Ile Ile Lys 
                565                 570                 575     


Glu Leu Trp Glu Asn Thr Lys Gly Arg Asp Gly Leu Leu Val Ala Asp 
            580                 585                 590         


Gly Gly Pro Asn Ile Tyr Asn Leu Arg Asn Leu His Ile Pro Glu Ile 
        595                 600                 605             


Val Leu Lys Tyr Asn Leu Met Asp Pro Glu Tyr Lys Gly Arg Leu Leu 
    610                 615                 620                 


His Pro Gln Asn Pro Phe Val Gly His Leu Ser Ile Glu Gly Ile Lys 
625                 630                 635                 640 


Glu Ala Asp Ile Thr Pro Ala His Gly Pro Val Arg Lys Met Asp Tyr 
                645                 650                 655     


Asp Ala Val Ser Gly Thr His Ser Trp Arg Thr Lys Arg Asn Arg Ser 
            660                 665                 670         


Ile Leu Asn Thr Asp Gln Arg Asn Met Ile Leu Glu Glu Gln Cys Tyr 
        675                 680                 685             


Ala Lys Cys Cys Asn Leu Phe Glu Ala Cys Phe Asn Ser Ala Ser Tyr 
    690                 695                 700                 


Arg Lys Pro Val Gly Gln His Ser Met Leu Glu Ala Met Ala His Arg 
705                 710                 715                 720 


Leu Arg Met Asp Ala Arg Leu Asp Tyr Glu Ser Gly Arg Met Ser Lys 
                725                 730                 735     


Asp Asp Phe Glu Lys Ala Met Ala His Leu Gly Glu Ile Gly Tyr Thr 
            740                 745                 750         


<210>  23
<211>  248
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Thueringen/02/06, amino acid sequence M1 K93R

<400>  23

Met Ser Leu Phe Gly Asp Thr Ile Ala Tyr Leu Leu Ser Leu Thr Glu 
1               5                   10                  15      


Asp Gly Glu Gly Lys Ala Glu Leu Ala Glu Lys Leu His Cys Trp Phe 
            20                  25                  30          


Gly Gly Lys Glu Phe Asp Leu Asp Ser Ala Leu Glu Trp Ile Lys Asn 
        35                  40                  45              


Lys Arg Cys Leu Thr Asp Ile Gln Lys Ala Leu Ile Gly Ala Ser Ile 
    50                  55                  60                  


Cys Phe Leu Lys Pro Lys Asp Gln Glu Arg Lys Arg Arg Phe Ile Thr 
65                  70                  75                  80  


Glu Pro Leu Ser Gly Met Gly Thr Thr Ala Thr Lys Arg Lys Gly Leu 
                85                  90                  95      


Ile Leu Ala Glu Arg Lys Met Arg Lys Cys Val Ser Phe His Glu Ala 
            100                 105                 110         


Phe Glu Ile Ala Glu Gly His Glu Ser Ser Ala Leu Leu Tyr Cys Leu 
        115                 120                 125             


Met Val Met Tyr Leu Asn Pro Gly Asn Tyr Ser Met Gln Val Lys Leu 
    130                 135                 140                 


Gly Thr Leu Cys Ala Leu Cys Glu Lys Gln Ala Ser His Ser His Arg 
145                 150                 155                 160 


Ala His Ser Arg Ala Ala Arg Ser Ser Val Pro Gly Val Arg Arg Glu 
                165                 170                 175     


Met Gln Met Val Ser Ala Met Asn Thr Ala Lys Thr Met Asn Gly Met 
            180                 185                 190         


Gly Lys Gly Glu Asp Val Gln Lys Leu Ala Glu Glu Leu Gln Ser Asn 
        195                 200                 205             


Ile Gly Val Leu Arg Ser Leu Gly Ala Ser Gln Lys Asn Gly Glu Gly 
    210                 215                 220                 


Ile Ala Lys Asp Val Met Glu Val Leu Lys Gln Ser Ser Met Gly Asn 
225                 230                 235                 240 


Ser Ala Leu Val Lys Lys Tyr Leu 
                245             


<210>  24
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B/Thueringen/02/06 BGHB ?NS, amino acid sequence NS2 Y117H

<400>  24

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Trp Arg Met Lys Lys 
1               5                   10                  15      


Met Ala Ile Gly Ser Ser Ile His Ser Ser Ser Val Leu Met Lys Asp 
            20                  25                  30          


Ile Gln Ser Gln Phe Glu Gln Leu Lys Leu Arg Trp Glu Ser Tyr Pro 
        35                  40                  45              


Asn Leu Val Lys Ser Thr Asp Tyr His Gln Lys Arg Glu Thr Ile Arg 
    50                  55                  60                  


Leu Val Thr Glu Glu Leu Tyr Leu Leu Ser Lys Arg Ile Asp Asp Asn 
65                  70                  75                  80  


Ile Leu Phe His Lys Thr Val Ile Ala Asn Ser Ser Ile Ile Ala Asp 
                85                  90                  95      


Met Val Val Ser Leu Ser Leu Leu Glu Thr Leu Tyr Glu Met Lys Asp 
            100                 105                 110         


Val Val Glu Val His Ser Arg 
        115                 


<210>  25
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  protein

<400>  25

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Val Gly Pro Gly Ala 
1               5                   10                  15      


Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
            20                  25                  30          


Glu Glu Asn Pro Gly Pro Met Lys Thr Asp Thr Leu Leu Leu Trp Val 
        35                  40                  45              


Leu Leu Leu Trp Val Pro Arg Ser His Gly Asn Ile Thr Asn Leu Cys 
    50                  55                  60                  


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
65                  70                  75                  80  


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
                85                  90                  95      


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
            100                 105                 110         


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
        115                 120                 125             


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
    130                 135                 140                 


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
145                 150                 155                 160 


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
                165                 170                 175     


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
            180                 185                 190         


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
        195                 200                 205             


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
    210                 215                 220                 


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
225                 230                 235                 240 


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
                245                 250                 255     


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn 
            260                 265                 270         


Gly Leu Thr Gly Thr 
        275         


<210>  26
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminally truncated NS1

<400>  26

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Val Gly Pro Gly Ala 
1               5                   10                  15      


<210>  27
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  signal peptide

<400>  27

Met Lys Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Arg Ser His Gly 
            20  


<210>  28
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD sequence

<400>  28

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  29
<211>  657
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD219 (nt 22553-23209 in SARS-CoV-2 reference strain 
       hCoV-19/Wuhan/Hu-1/2019 EPI ISL 402125, S Protein is nt 
       21536-25384)

<400>  29
aatattacaa acttgtgccc ttttggtgaa gtttttaacg ccaccagatt tgcatctgtt       60

tatgcttgga acaggaagag aatcagcaac tgtgttgctg attattctgt cctatataat      120

tccgcatcat tttccacttt taagtgttat ggagtgtctc ctactaaatt aaatgatctc      180

tgctttacta atgtctatgc agattcattt gtaattagag gtgatgaagt cagacaaatc      240

gctccagggc aaactggaaa gattgctgat tataattata aattaccaga tgattttaca      300

ggctgcgtta tagcttggaa ttctaacaat cttgattcta aggttggtgg taattataat      360

tacctgtata gattgtttag gaagtctaat ctcaaacctt ttgagagaga tatttcaact      420

gaaatctatc aggccggtag cacaccttgt aatggtgttg aaggttttaa ttgttacttt      480

cctttacaat catatggttt ccaacccact aatggtgttg gttaccaacc atacagagta      540

gtagtacttt cttttgaact tctacatgca ccagcaactg tttgtggacc taaaaagtct      600

actaatttgg ttaaaaacaa atgtgtcaat ttcaacttca atggtttaac aggcaca         657


<210>  30
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence is AA 331-524 in the S Protein of the 
       reference strain

<400>  30

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  31
<211>  669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD223 (nt 22517- 23185 in SARS-CoV-2 reference strain 
       hCoV-19/Wuhan/Hu-1/2019 EPI ISL 402125, S Protein is nt 
       21536-25384)

<400>  31
agagtccagc cgaccgaatc tattgttaga tttcctaata tcacgaatct gtgccccttt       60

ggagaggtgt tcaatgctac ccgctttgct tcagtgtacg cttggaatag gaaacggatc      120

agtaattgcg tggctgatta ttctgtgctg tacaatagcg caagcttcag cacattcaaa      180

tgctatggag tgagccccac aaaactgaat gatctgtgct tcactaatgt ttacgccgat      240

tcatttgtga tacgcggaga tgaggtgaga cagattgccc ctggccaaac aggaaaaatc      300

gcggattaca attacaaact gccagatgat ttcactggat gcgtgattgc atggaattca      360

aataatctgg atagtaaagt tggaggcaat tacaattacc tgtacagact gttcagaaaa      420

agcaatctga aaccctttga gcgagatatc agcaccgaaa tctaccaggc tggctctacg      480

ccttgcaatg gagtggaggg attcaattgt tattttcctc tgcagagtta cggattccaa      540

ccgaccaatg gtgtgggata tcagccctac agagtggtgg tgctgagctt cgaactgctt      600

cacgctccag caacagtgtg cggacccaaa aaatctacta atctggtgaa aaataagtgc      660

gtgaatttc                                                              669


<210>  32
<211>  223
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid Sequence is AA 319-514 in the S protein of the 
       reference strain

<400>  32

Arg Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn 
1               5                   10                  15      


Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val 
            20                  25                  30          


Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser 
        35                  40                  45              


Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val 
    50                  55                  60                  


Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp 
65                  70                  75                  80  


Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln 
                85                  90                  95      


Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr 
            100                 105                 110         


Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly 
        115                 120                 125             


Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys 
    130                 135                 140                 


Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr 
145                 150                 155                 160 


Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser 
                165                 170                 175     


Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val 
            180                 185                 190         


Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly 
        195                 200                 205             


Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe 
    210                 215                 220             


<210>  33
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid: Transmembrane Domain

<400>  33

Leu Leu Tyr Tyr Ser Thr Ala Ala Ser Ser Leu Ala Val Thr Leu Met 
1               5                   10                  15      


Leu Ala Ile Phe Ile Val Tyr Met Val Ser 
            20                  25      


<210>  34
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino Acid: Cytoplasmic Tail

<400>  34

Arg Asp Asn Val Ser Cys Ser Ile Cys Leu 
1               5                   10  


<210>  35
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide: Transmembrane Domain

<400>  35
ctgctctatt actcaactgc tgcttctagt ttggctgtaa cattaatgct agctattttt       60

attgtttata tggtctcc                                                     78


<210>  36
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleotide: Cytoplasmic Tail

<400>  36
agagacaacg tttcatgctc catctgtcta                                        30


<210>  37
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flu A (A/Texas/04/2009) HA2 Transmembrane domain and Cytoplasmic 
       Tail

Amino Acid:

Transmembrane Domain

<400>  37

Gln Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Val 
1               5                   10                  15      


Val Ser Leu Gly Ala Ile Ser Phe Trp Met Cys 
            20                  25          


<210>  38
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flu A (A/Texas/04/2009) HA2 Transmembrane domain and Cytoplasmic 
       Tail

Amino Acid:

Cytoplasmic Tail

<400>  38

Ser Asn Gly Ser Leu Gln Cys Arg Ile Cys Ile 
1               5                   10      


<210>  39
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flu A (A/Texas/04/2009) HA2 Transmembrane domain and Cytoplasmic 
       Tail Nucleotide:

Transmembrane Domain

<400>  39
agattttggc gatctattca actgtcgcca gttcattggt actggtagtc tccctggggg       60

caatcagttt ctggatgtgc                                                   80


<210>  40
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Flu A (A/Texas/04/2009) HA2 Transmembrane domain and Cytoplasmic 
       Tail Nucleotide: Cytoplasmic Tail

<400>  40
tctaatgggt ctctacagtg tagaatatgt att                                    33


<210>  41
<211>  1442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BGHB delNS1-RBD219 BSMBI plus Flu B HA2 linker, transmembrane 
       domain, and cytoplasmic tail from B/Phuket/3073/2013 (Codon 
       optimized) nucleotide sequence

<400>  41
agggagcaga agcagaggat ttgtttagtc actggcaaac aggaaaaatg gcggacaata       60

tgaccacaac acaaattgag gtgggtccgg gagcaggatc cggagcaaca aatttctcac      120

ttcttaaaca agcaggggac gtggaagaaa atccaggacc aatgaaaaca gatacacttc      180

ttctttgggt gctgcttttg tgggttccaa gatcacatgg gaatatcacg aatctgtgcc      240

cctttggaga ggtgttcaat gctacccgct ttgcttcagt gtacgcttgg aataggaaac      300

ggatcagtaa ttgcgtggct gattattctg tgctgtacaa tagcgcaagc ttcagcacat      360

tcaaatgcta tggagtgagc cccacaaaac tgaatgatct gtgcttcact aatgtttacg      420

ccgattcatt tgtgatacgc ggagatgagg tgagacagat tgcccctggc caaacaggaa      480

aaatcgcgga ttacaattac aaactgccag atgatttcac tggatgcgtg attgcatgga      540

attcaaataa tctggatagt aaagttggag gcaattacaa ttacctgtac agactgttca      600

gaaaaagcaa tctgaaaccc tttgagcgag atatcagcac cgaaatctac caggctggct      660

ctacgccttg caatggagtg gagggattca attgttattt tcctctgcag agttacggat      720

tccaaccgac caatggtgtg ggatatcagc cctacagagt ggtggtgctg agcttcgaac      780

tgcttcacgc tccagcaaca gtgtgcggac ccaaaaaatc tactaatctg gtgaaaaata      840

agtgcgtgaa tttcaatttc aatggactga cgggaacggg attggataat catactatac      900

tgctttacta ctcaactgct gcctccagtt tggctgtaac actgatgata gctatctttg      960

ttgtttatat ggtctccaga gacaatgttt cttgctccat ctgtctataa tgatgagcgg     1020

ccgcccaagc agaaagtggt actaaccttc ttctctttct tctcctgaca gtggaggatg     1080

aagaagatgg ccatcggatc ctcaattcac tcttcgagcg tcttaatgaa ggacattcaa     1140

agccaattcg agcagctgaa actgcggtgg gagtcttatc ccaatttggt caagagcacc     1200

gattatcacc agaagaggga gacaattaga ctggtcacgg aagaacttta tcttttaagt     1260

aaaagaattg atgataacat attgttccac aaaacagtaa tagctaacag ctccataata     1320

gctgacatgg ttgtatcatt atcattatta gaaacattgt atgaaatgaa ggatgtggtt     1380

gaagtgtaca gcaggcagtg cttgtgaatt taaaataaaa atcctcttgt tactactaat     1440

ac                                                                    1442


<210>  42
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BGHB delNS1-RBD219 BSMBI plus Flu B HA2 linker, transmembrane 
       domain, and cytoplasmic tail from B/Phuket/3073/2013 (Codon 
       optimized) amino acid sequence NEP/NS2 (spliced)

<400>  42

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Trp Arg Met Lys Lys 
1               5                   10                  15      


Met Ala Ile Gly Ser Ser Ile His Ser Ser Ser Val Leu Met Lys Asp 
            20                  25                  30          


Ile Gln Ser Gln Phe Glu Gln Leu Lys Leu Arg Trp Glu Ser Tyr Pro 
        35                  40                  45              


Asn Leu Val Lys Ser Thr Asp Tyr His Gln Lys Arg Glu Thr Ile Arg 
    50                  55                  60                  


Leu Val Thr Glu Glu Leu Tyr Leu Leu Ser Lys Arg Ile Asp Asp Asn 
65                  70                  75                  80  


Ile Leu Phe His Lys Thr Val Ile Ala Asn Ser Ser Ile Ile Ala Asp 
                85                  90                  95      


Met Val Val Ser Leu Ser Leu Leu Glu Thr Leu Tyr Glu Met Lys Asp 
            100                 105                 110         


Val Val Glu Val Tyr Ser Arg Gln Cys Leu 
        115                 120         


<210>  43
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD219

<400>  43

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  44
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flu B HA2 Linker

<400>  44

Gly Leu Asp Asn His Thr Ile 
1               5           


<210>  45
<211>  1448
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BGHB delNS1-RBD219 BSMBI plus generic linker, Flu B HA2 
       transmembrane domain, and cytoplasmic tail from 
       B/Phuket/3073/2013 (Codon optimized) nucleotide sequence

<400>  45
agggagcaga agcagaggat ttgtttagtc actggcaaac aggaaaaatg gcggacaata       60

tgaccacaac acaaattgag gtgggtccgg gagcaggatc cggagcaaca aatttctcac      120

ttcttaaaca agcaggggac gtggaagaaa atccaggacc aatgaaaaca gatacacttc      180

ttctttgggt gctgcttttg tgggttccaa gatcacatgg gaatatcacg aatctgtgcc      240

cctttggaga ggtgttcaat gctacccgct ttgcttcagt gtacgcttgg aataggaaac      300

ggatcagtaa ttgcgtggct gattattctg tgctgtacaa tagcgcaagc ttcagcacat      360

tcaaatgcta tggagtgagc cccacaaaac tgaatgatct gtgcttcact aatgtttacg      420

ccgattcatt tgtgatacgc ggagatgagg tgagacagat tgcccctggc caaacaggaa      480

aaatcgcgga ttacaattac aaactgccag atgatttcac tggatgcgtg attgcatgga      540

attcaaataa tctggatagt aaagttggag gcaattacaa ttacctgtac agactgttca      600

gaaaaagcaa tctgaaaccc tttgagcgag atatcagcac cgaaatctac caggctggct      660

ctacgccttg caatggagtg gagggattca attgttattt tcctctgcag agttacggat      720

tccaaccgac caatggtgtg ggatatcagc cctacagagt ggtggtgctg agcttcgaac      780

tgcttcacgc tccagcaaca gtgtgcggac ccaaaaaatc tactaatctg gtgaaaaata      840

agtgcgtgaa tttcaatttc aatggactga cgggaacggg tagtggtagt ggtagtggta      900

gtggtctgct ttactactca actgctgcct ccagtttggc tgtaacactg atgatagcta      960

tctttgttgt ttatatggtc tccagagaca atgtttcttg ctccatctgt ctataatgat     1020

gagcggccgc ccaagcagaa agtggtacta accttcttct ctttcttctc ctgacagtgg     1080

aggatgaaga agatggccat cggatcctca attcactctt cgagcgtctt aatgaaggac     1140

attcaaagcc aattcgagca gctgaaactg cggtgggagt cttatcccaa tttggtcaag     1200

agcaccgatt atcaccagaa gagggagaca attagactgg tcacggaaga actttatctt     1260

ttaagtaaaa gaattgatga taacatattg ttccacaaaa cagtaatagc taacagctcc     1320

ataatagctg acatggttgt atcattatca ttattagaaa cattgtatga aatgaaggat     1380

gtggttgaag tgtacagcag gcagtgcttg tgaatttaaa ataaaaatcc tcttgttact     1440

actaatac                                                              1448


<210>  46
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BGHB delNS1-RBD219 BSMBI plus generic linker, Flu B HA2 
       transmembrane domain, and cytoplasmic tail from 
       B/Phuket/3073/2013 (Codon optimized) amino acid sequence NEP/NS2 
       (spliced)

<400>  46

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Trp Arg Met Lys Lys 
1               5                   10                  15      


Met Ala Ile Gly Ser Ser Ile His Ser Ser Ser Val Leu Met Lys Asp 
            20                  25                  30          


Ile Gln Ser Gln Phe Glu Gln Leu Lys Leu Arg Trp Glu Ser Tyr Pro 
        35                  40                  45              


Asn Leu Val Lys Ser Thr Asp Tyr His Gln Lys Arg Glu Thr Ile Arg 
    50                  55                  60                  


Leu Val Thr Glu Glu Leu Tyr Leu Leu Ser Lys Arg Ile Asp Asp Asn 
65                  70                  75                  80  


Ile Leu Phe His Lys Thr Val Ile Ala Asn Ser Ser Ile Ile Ala Asp 
                85                  90                  95      


Met Val Val Ser Leu Ser Leu Leu Glu Thr Leu Tyr Glu Met Lys Asp 
            100                 105                 110         


Val Val Glu Val Tyr Ser Arg Gln Cys Leu 
        115                 120         


<210>  47
<211>  219
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD219

<400>  47

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys 
        195                 200                 205             


Val Asn Phe Asn Phe Asn Gly Leu Thr Gly Thr 
    210                 215                 


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Generic Linker

<400>  48

Gly Ser Gly Ser Gly Ser Gly Ser Gly 
1               5                   


<210>  49
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal domain sequence is nearly 100% conserved across all 
       IAV subtypes

<400>  49

Met Asn Pro Asn Gln Lys 
1               5       


<210>  50
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza B Thueringen/02/06 wild-type NEP/NS2 amino acid 
       sequence

<400>  50

Met Ala Asp Asn Met Thr Thr Thr Gln Ile Glu Trp Arg Met Lys Lys 
1               5                   10                  15      


Met Ala Ile Gly Ser Ser Ile His Ser Ser Ser Val Leu Met Lys Asp 
            20                  25                  30          


Ile Gln Ser Gln Phe Glu Gln Leu Lys Leu Arg Trp Glu Ser Tyr Pro 
        35                  40                  45              


Asn Leu Val Lys Ser Thr Asp Tyr His Gln Lys Arg Glu Thr Ile Arg 
    50                  55                  60                  


Leu Val Thr Glu Glu Leu Tyr Leu Leu Ser Lys Arg Ile Asp Asp Asn 
65                  70                  75                  80  


Ile Leu Phe His Lys Thr Val Ile Ala Asn Ser Ser Ile Ile Ala Asp 
                85                  90                  95      


Met Val Val Ser Leu Ser Leu Leu Glu Thr Leu Tyr Glu Met Lys Asp 
            100                 105                 110         


Val Val Glu Val Tyr Ser Arg Gln Cys Leu 
        115                 120         


<210>  51
<211>  890
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Influenza A/Puerto Rico/8/34 wild-type NS segment nucleotide 
       sequence

<400>  51
agcaaaagca gggtgacaaa gacataatgg atccaaacac tgtgtcaagc tttcaggtag       60

attgctttct ttggcatgtc cgcaaacgag ttgcagacca agaactaggt gatgccccat      120

tccttgatcg gcttcgccga gatcagaaat ccctaagagg aaggggcagc accctcggtc      180

tggacatcga gacagccaca cgtgctggaa agcagatagt ggagcggatt ctgaaagaag      240

aatccgatga ggcacttaaa atgaccatgg cctctgtacc tgcgtcgcgt tacctaactg      300

acatgactct tgaggaaatg tcaagggact ggtccatgct catacccaag cagaaagtgg      360

caggccctct ttgtatcaga atggaccagg cgatcatgga taagaacatc atactgaaag      420

cgaacttcag tgtgattttt gaccggctgg agactctaat attgctaagg gctttcaccg      480

aagagggagc aattgttggc gaaatttcac cattgccttc tcttccagga catactgctg      540

aggatgtcaa aaatgcagtt ggagtcctca tcgggggact tgaatggaat gataacacag      600

ttcgagtctc tgaaactcta cagagattcg cttggagaag cagtaatgag aatgggagac      660

ctccactcac tccaaaacag aaacgagaaa tggcgggaac aattaggtca gaagtttgaa      720

gaaataagat ggttgattga agaagtgaga cacaaactga agataacaga gaatagtttt      780

gagcaaataa catttatgca agccttacat ctattgcttg aagtggagca agagataaga      840

actttctcgt ttcagcttat ttaataataa aaaacaccct tgtttctact                 890


<210>  52
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza A/Puerto Rico/8/34 wild-type NS1 amino acid sequence

<400>  52

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Val Asp Cys Phe Leu Trp 
1               5                   10                  15      


His Val Arg Lys Arg Val Ala Asp Gln Glu Leu Gly Asp Ala Pro Phe 
            20                  25                  30          


Leu Asp Arg Leu Arg Arg Asp Gln Lys Ser Leu Arg Gly Arg Gly Ser 
        35                  40                  45              


Thr Leu Gly Leu Asp Ile Glu Thr Ala Thr Arg Ala Gly Lys Gln Ile 
    50                  55                  60                  


Val Glu Arg Ile Leu Lys Glu Glu Ser Asp Glu Ala Leu Lys Met Thr 
65                  70                  75                  80  


Met Ala Ser Val Pro Ala Ser Arg Tyr Leu Thr Asp Met Thr Leu Glu 
                85                  90                  95      


Glu Met Ser Arg Asp Trp Ser Met Leu Ile Pro Lys Gln Lys Val Ala 
            100                 105                 110         


Gly Pro Leu Cys Ile Arg Met Asp Gln Ala Ile Met Asp Lys Asn Ile 
        115                 120                 125             


Ile Leu Lys Ala Asn Phe Ser Val Ile Phe Asp Arg Leu Glu Thr Leu 
    130                 135                 140                 


Ile Leu Leu Arg Ala Phe Thr Glu Glu Gly Ala Ile Val Gly Glu Ile 
145                 150                 155                 160 


Ser Pro Leu Pro Ser Leu Pro Gly His Thr Ala Glu Asp Val Lys Asn 
                165                 170                 175     


Ala Val Gly Val Leu Ile Gly Gly Leu Glu Trp Asn Asp Asn Thr Val 
            180                 185                 190         


Arg Val Ser Glu Thr Leu Gln Arg Phe Ala Trp Arg Ser Ser Asn Glu 
        195                 200                 205             


Asn Gly Arg Pro Pro Leu Thr Pro Lys Gln Lys Arg Glu Met Ala Gly 
    210                 215                 220                 


Thr Ile Arg Ser Glu Val 
225                 230 


<210>  53
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Influenza A/Puerto Rico/8/34 wild-type NEP/NS2 amino acid 
       sequence

<400>  53

Met Asp Pro Asn Thr Val Ser Ser Phe Gln Asp Ile Leu Leu Arg Met 
1               5                   10                  15      


Ser Lys Met Gln Leu Glu Ser Ser Ser Gly Asp Leu Asn Gly Met Ile 
            20                  25                  30          


Thr Gln Phe Glu Ser Leu Lys Leu Tyr Arg Asp Ser Leu Gly Glu Ala 
        35                  40                  45              


Val Met Arg Met Gly Asp Leu His Ser Leu Gln Asn Arg Asn Glu Lys 
    50                  55                  60                  


Trp Arg Glu Gln Leu Gly Gln Lys Phe Glu Glu Ile Arg Trp Leu Ile 
65                  70                  75                  80  


Glu Glu Val Arg His Lys Leu Lys Ile Thr Glu Asn Ser Phe Glu Gln 
                85                  90                  95      


Ile Thr Phe Met Gln Ala Leu His Leu Leu Leu Glu Val Glu Gln Glu 
            100                 105                 110         


Ile Arg Thr Phe Ser Phe Gln Leu Ile 
        115                 120     


<210>  54
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD-219 Original Sequence

<400>  54

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val 
        


<210>  55
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD-219 Original Sequence: England Variant: N501Y

<400>  55

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val 
        


<210>  56
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD-219 Original Sequence: South Africa Variant: 
       K417N, E484K and N501Y

<400>  56

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Asn Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Thr Pro Cys Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val 
        


<210>  57
<211>  194
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SARS-CoV-2 RBD-219 Delta Variant: L452R and T478K

<400>  57

Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg 
1               5                   10                  15      


Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val 
            20                  25                  30          


Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys 
        35                  40                  45              


Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn 
    50                  55                  60                  


Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile 
65                  70                  75                  80  


Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro 
                85                  90                  95      


Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp 
            100                 105                 110         


Ser Lys Val Gly Gly Asn Tyr Asn Tyr Arg Tyr Arg Leu Phe Arg Lys 
        115                 120                 125             


Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln 
    130                 135                 140                 


Ala Gly Ser Lys Pro Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe 
145                 150                 155                 160 


Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln 
                165                 170                 175     


Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala 
            180                 185                 190         


Thr Val 
        


