                         SEQUENCE LISTING

<110>  NATIONAL INSTITUTE OF PLANT GENOMIC RESEARCH
 
<120>  METHODS FOR PRODUCING TRANSGENIC PLANTS OVEREXPRESSING 
       NON-SYMBIOTIC HEMOGLOBIN CLASS-1 GENE, AND APPLICATIONS THEREOF

<130>  PD037539IN-SC

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  depicts the nucleic acid sequence encoding non-symbiotic 
       hemoglobin.

<400>  1
atgagtagct ttagtgaaga acaagaagct ttggtagtca aatcatgggg gtctatgaag       60

aaggatgctg gtgaatgggg tctcaaattc tttctcaaga tatttgaaat agcaccatca      120

gcaaaaaaga tgttctcatt cctcaaagat tcaaatgtgc cattggatca aaatcctaag      180

ctcaagattc atgctaaatc tgtccttgtt atgacttgtg aagcggcagt tcaacttaga      240

aaagctggaa aggttgtggt gagggattcc actctcaaaa aaataggtgc tacacacttt      300

aagtatggtg tggttgatga gcactttgag gtaacaaaat atgccttgtt ggagacaata      360

aaagaagcaa gtcaagaaat gtggagtgtt gagatgaaga atgcatgggg agaggcctat      420

gatcaattgg ttagtgctat caagactgag atgaagtag                             459


<210>  2
<211>  152
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  depicts the amino acid sequence of non-symbiotic hemoglobin class
       1 gene (nsHb1).

<400>  2

Met Ser Ser Phe Ser Glu Glu Gln Glu Ala Leu Val Val Lys Ser Trp 
1               5                   10                  15      


Gly Ser Met Lys Lys Asp Ala Gly Glu Trp Gly Leu Lys Phe Phe Leu 
            20                  25                  30          


Lys Ile Phe Glu Ile Ala Pro Ser Ala Lys Lys Met Phe Ser Phe Leu 
        35                  40                  45              


Lys Asp Ser Asn Val Pro Leu Asp Gln Asn Pro Lys Leu Lys Ile His 
    50                  55                  60                  


Ala Lys Ser Val Leu Val Met Thr Cys Glu Ala Ala Val Gln Leu Arg 
65                  70                  75                  80  


Lys Ala Gly Lys Val Val Val Arg Asp Ser Thr Leu Lys Lys Ile Gly 
                85                  90                  95      


Ala Thr His Phe Lys Tyr Gly Val Val Asp Glu His Phe Glu Val Thr 
            100                 105                 110         


Lys Tyr Ala Leu Leu Glu Thr Ile Lys Glu Ala Ser Gln Glu Met Trp 
        115                 120                 125             


Ser Val Glu Met Lys Asn Ala Trp Gly Glu Ala Tyr Asp Gln Leu Val 
    130                 135                 140                 


Ser Ala Ile Lys Thr Glu Met Lys 
145                 150         


<210>  3
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  depicts the forward primer nucleic acid sequence used for 
       amplification of Solanum lycopersicum non-symbiotic hemoglobin 
       class 1 gene (SlNSH1).

<400>  3
caccatgagt agctttagtg aaga                                              24


<210>  4
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  depicts the reverse primer nucleic acid sequence used for 
       amplification of Solanum lycopersicum non-symbiotic hemoglobin 
       class 1 gene (SlNSH1).

<400>  4
tagcacacaa attagattat                                                   20


