               SEQUENCE LISTING

<110> Novartis Pharma AG

<120> Expression technology for antibody constructs

<130> PAT059005-WO-PCT

<150> US 63/117,379
<151> 2020-11-23

<160> 25

<170> BiSSAP 1.3.6

<210> 1
<211> 98
<212> PRT
<213> Homo sapiens


<400> 1
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val 
        

<210> 2
<211> 12
<212> PRT
<213> Homo sapiens


<400> 2
Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro 
1               5                   10          

<210> 3
<211> 113
<212> PRT
<213> Homo sapiens


<400> 3
Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe 
1               5                   10                  15      
Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val 
            20                  25                  30          
Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe 
        35                  40                  45              
Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro 
    50                  55                  60                  
Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr 
65                  70                  75                  80  
Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val 
                85                  90                  95      
Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala 
            100                 105                 110         
Lys 
    

<210> 4
<211> 107
<212> PRT
<213> Homo sapiens


<400> 4
Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp 
1               5                   10                  15      
Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          
Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              
Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  
Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly 
65                  70                  75                  80  
Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      
Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            100                 105         

<210> 5
<211> 330
<212> PRT
<213> Homo sapiens


<400> 5
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     
Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 

<210> 6
<211> 106
<212> PRT
<213> Homo sapiens


<400> 6
Gly Gln Pro Lys Ala Asn Pro Thr Val Thr Leu Phe Pro Pro Ser Ser 
1               5                   10                  15      
Glu Glu Leu Gln Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp 
            20                  25                  30          
Phe Tyr Pro Gly Ala Val Thr Val Ala Trp Lys Ala Asp Gly Ser Pro 
        35                  40                  45              
Val Lys Ala Gly Val Glu Thr Thr Lys Pro Ser Lys Gln Ser Asn Asn 
    50                  55                  60                  
Lys Tyr Ala Ala Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys 
65                  70                  75                  80  
Ser His Arg Ser Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val 
                85                  90                  95      
Glu Lys Thr Val Ala Pro Thr Glu Cys Ser 
            100                 105     

<210> 7
<211> 107
<212> PRT
<213> Homo sapiens


<400> 7
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         

<210> 8
<211> 8
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide consensus sequence

<220> 
<221> VARIANT
<222> 2
<223> Xaa may be any amino acid

<220> 
<221> VARIANT
<222> 4
<223> Xaa may be any amino acid

<400> 8
Asp Xaa Glu Xaa Asn Pro Gly Pro 
1               5               

<210> 9
<211> 13
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide consensus sequence

<220> 
<221> VARIANT
<222> 2
<223> Xaa may be any amino acid

<220> 
<221> VARIANT
<222> 3
<223> Xaa may be any amino acid

<220> 
<221> VARIANT
<222> 4
<223> Xaa may be any amino acid

<220> 
<221> VARIANT
<222> 9
<223> Xaa may be any amino acid

<400> 9
Leu Xaa Xaa Xaa Gly Asp Val Glu Xaa Asn Pro Gly Pro 
1               5                   10              

<210> 10
<211> 24
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of foot-and-mouth disease virus

<400> 10
Ala Pro Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly 
1               5                   10                  15      
Asp Val Glu Ser Asn Pro Gly Pro 
            20                  

<210> 11
<211> 33
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of foot-and-mouth disease virus

<400> 11
Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val Lys Gln Thr Leu 
1               5                   10                  15      
Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu Ser Asn Pro Gly 
            20                  25                  30          
Pro 
    

<210> 12
<211> 19
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of porcine teschovirus-1

<400> 12
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn 
1               5                   10                  15      
Pro Gly Pro 
            

<210> 13
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of equine rhinitis A virus

<400> 13
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 14
<211> 18
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of Thosea asigna virus

<400> 14
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro 
1               5                   10                  15      
Gly Pro 
        

<210> 15
<211> 20
<212> PRT
<213> Artificial Sequence


<220> 
<223> 2A peptide of Thosea asigna virus

<400> 15
Arg Ala Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 16
<211> 25
<212> PRT
<213> Strongylocentrotus purpuratus


<400> 16
Asp Gly Phe Cys Ile Leu Tyr Leu Leu Leu Ile Leu Leu Met Arg Ser 
1               5                   10                  15      
Gly Asp Val Glu Thr Asn Pro Gly Pro 
            20                  25  

<210> 17
<211> 20
<212> PRT
<213> Amphimedon queenslandica


<400> 17
Leu Leu Cys Phe Met Leu Leu Leu Leu Leu Ser Gly Asp Val Glu Leu 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 18
<211> 20
<212> PRT
<213> Amphimedon queenslandica


<400> 18
His His Phe Met Phe Leu Leu Leu Leu Leu Ala Gly Asp Ile Glu Leu 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 19
<211> 19
<212> PRT
<213> Saccoglossus kowalevskii


<400> 19
Trp Phe Leu Val Leu Leu Ser Phe Ile Leu Ser Gly Asp Ile Glu Val 
1               5                   10                  15      
Asn Pro Gly 
            

<210> 20
<211> 20
<212> PRT
<213> Branchiostoma floridae


<400> 20
Lys Asn Cys Ala Met Tyr Met Leu Leu Leu Ser Gly Asp Val Glu Thr 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 21
<211> 20
<212> PRT
<213> Branchiostoma floridae


<400> 21
Met Val Ile Ser Gln Leu Met Leu Lys Leu Ala Gly Asp Val Glu Glu 
1               5                   10                  15      
Asn Pro Gly Pro 
            20  

<210> 22
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> furin recognition site

<220> 
<221> VARIANT
<222> 2
<223> Xaa may be any amino acid

<220> 
<221> VARIANT
<222> 3
<223> Xaa may be Arg or Lys

<400> 22
Arg Xaa Xaa Arg 
1               

<210> 23
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> furin recognition site

<400> 23
Arg Lys Arg Arg 
1               

<210> 24
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> furin recognition site

<400> 24
Arg Arg Lys Arg 
1               

<210> 25
<211> 37
<212> PRT
<213> Artificial Sequence


<220> 
<223> furin/2A peptide linker

<400> 25
Arg Lys Arg Arg Glu Ala Arg His Lys Gln Lys Ile Val Ala Pro Val 
1               5                   10                  15      
Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val Glu 
            20                  25                  30          
Ser Asn Pro Gly Pro 
        35          

