                         SEQUENCE LISTING

<110>  WORLD BIOTECH REGENERATIVE MEDICAL GROUP LIMITED
       MAN, Wing Yin Cornelia
       MA, Wei
       GAO, Quangui
 
<120>  SYSTEMS FOR THE DETECTION OF TARGETED GENE VARIATIONS AND VIRAL 
       GENOMES AND METHODS OF PRODUCING AND USING SAME

<130>  10132739-50738774

<150>  US 63/114,844
<151>  2020-11-17

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs429358 (APOE) F3 primer

<400>  1
aggaacaact gaccccgg                                                     18


<210>  2
<211>  13
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs429358 (APOE) B3 primer

<400>  2
ggcgcacccg cag                                                          13


<210>  3
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs429358 (APOE) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  3
gaacgtcctc catgtccgcg gcacggctgt ccaagg                                 36


<210>  4
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs429358 (APOE) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  4
ctcggccgcc tggtgcagta gctctggccg agcatg                                 36


<210>  5
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs429358 (APOE) LoopF primer

<400>  5
gcgccgcctg cagct                                                        15


<210>  6
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7412 (APOE) F3 primer

<400>  6
cggccagagc accg                                                         14


<210>  7
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7412 (APOE) B3 primer

<400>  7
cacgcggccc tgttcc                                                       16


<210>  8
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7412 (APOE) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  8
agttctgcag gtcatcggca gcctcgcctc ccacc                                  35


<210>  9
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7412 (APOE) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  9
tccctggcag tgtaccaggc gcgctcgcgg atggc                                  35


<210>  10
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7412 (APOE) LoopF primer

<400>  10
ccgcttacgc agcttgc                                                      17


<210>  11
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4343 (ACE) F3 primer

<400>  11
gaaattctct gagctcccct t                                                 21


<210>  12
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4343 (ACE) B3 primer

<400>  12
tttcgggtaa aactggagga tg                                                22


<210>  13
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4343 (ACE) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  13
tctggccatc acattcgtca caagcagagg tgagctaagg                             40


<210>  14
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4343 (ACE) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  14
agcccggaaa tatgaagacc ctctccccgc cttgtct                                37


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4343 (ACE) LoopF primer

<400>  15
ggggtttgaa tgccttgagc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs6265 (BDNF) F3 primer

<400>  16
tgactactga gcatcaccct                                                   20


<210>  17
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs6265 (BDNF) B3 primer

<400>  17
tgggactctg gagagcg                                                      17


<210>  18
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs6265 (BDNF) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  18
acgatagaag agctgttgga gacgtgtaca agtctgcgt                              39


<210>  19
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs6265 (BDNF) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  19
tctgttcgaa agtgtcagcc tgaatgggcc caaggcag                               38


<210>  20
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs6265 (BDNF) LoopF primer

<400>  20
ggcccaatga agaaaacaat aagg                                              24


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7349186 (PINK1) F3 primer

<400>  21
ccaacatcat ccgggttctc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7349186 (PINK1) B3 primer

<400>  22
tgggagatgt atcagctcca                                                   20


<210>  23
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7349186 (PINK1) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  23
ccgtggaggc gtgagggcag gccttcacct cttccgtg                               38


<210>  24
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7349186 (PINK1) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  24
gagaaggcct gggccatggc ccgcgctgct gtcact                                 36


<210>  25
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs7349186 (PINK1) LoopF primer

<400>  25
tcagggtagt cgaccaggg                                                    19


<210>  26
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs226249 (DJ1) F3 primer

<400>  26
gtgacgcagc gtgagg                                                       16


<210>  27
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs226249 (DJ1) B3 primer

<400>  27
cgctggcgct gacctg                                                       16


<210>  28
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs226249 (DJ1) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  28
cagaacgcac gccagcacgc caaggcggcg tgagtc                                 36


<210>  29
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs226249 (DJ1) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  29
gattcagcct ggtgtggggt tcttcctgcg gacgcg                                 36


<210>  30
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs226249 (DJ1) LoopF primer

<400>  30
cgtccggcct ccctca                                                       16


<210>  31
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs2736990 (SNCA) F3 primer

<400>  31
gatgatgagc aggcagtc                                                     18


<210>  32
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs2736990 (SNCA) B3 primer

<400>  32
acagatgact tagtaaatgt ctg                                               23


<210>  33
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs2736990 (SNCA) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  33
agttcttcct aaacagctca caattacata gtcagaaatc tgagt                       45


<210>  34
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs2736990 (SNCA) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  34
tcgtatatgt gtgtaacagg ttgcatcaga taatggctta c                           41


<210>  35
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs34637584 (LRRK2) F3 primer

<400>  35
gccatgatta tataccgaga cc                                                22


<210>  36
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs34637584 (LRRK2) B3 primer

<400>  36
cctgaagata gaattatgag acag                                              24


<210>  37
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs34637584 (LRRK2) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  37
tctagtcagc aatctttgca tgaaacccca caatgtgc                               38


<210>  38
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs34637584 (LRRK2) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  38
atcattgctc agtactgctg acctgatcac ctacctgg                               38


<210>  39
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs34637584 (LRRK2) LoopF primer

<400>  39
cattgggata cagtgtgaaa agca                                              24


<210>  40
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1871042 (GSTP1) F3 primer

<400>  40
agcaaagcag agcagacc                                                     18


<210>  41
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1871042 (GSTP1) B3 primer

<400>  41
gcatccaggc agccag                                                       16


<210>  42
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1871042 (GSTP1) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  42
tgcagggact cagccagcgg taggggatgg gcttaggc                               38


<210>  43
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1871042 (GSTP1) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  43
atcccctgcc ctgcagatct ctcatggatc agcagcaagt                             40


<210>  44
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1871042 (GSTP1) LoopF primer

<400>  44
aggaattggg ggcagagg                                                     18


<210>  45
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1050450 (GXT1) F3 primer

<400>  45
tgttcctccc tcgtaggtt                                                    19


<210>  46
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1050450 (GXT1) B3 primer

<400>  46
tgggccctga cggtgt                                                       16


<210>  47
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1050450 (GXT1) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  47
tgagctgtgc ctagggcgcc atagatgaaa acccccccga                             40


<210>  48
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1050450 (GXT1) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  48
atcccttgag acagcagggc agccgccgct tccaga                                 36


<210>  49
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1050450 (GXT1) LoopF primer

<400>  49
gcagttgcag tgctgctg                                                     18


<210>  50
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4880 (SOD2) F3 primer

<400>  50
tgcgcgttga tgtgagg                                                      17


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4880 (SOD2) B3 primer

<400>  51
cagcctgcgt agacggtc                                                     18


<210>  52
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4880 (SOD2) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  52
cattggggta tctgggctcc ttccagggcg ccgtag                                 36


<210>  53
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4880 (SOD2) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  53
gtcggagcca gctgcctgct cgcggcgctg actgac                                 36


<210>  54
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs4880 (SOD2) LoopF primer

<400>  54
aggcagaagc acagcctcc                                                    19


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1001179 (CAT) F3 primer

<400>  55
cagcagggtc taagtattcc g                                                 21


<210>  56
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1001179 (CAT) B3 primer

<400>  56
ccggagatac ccaggagg                                                     18


<210>  57
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1001179 (CAT) FIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  57
agtagccgaa cccagggcgg ggcaggccac caacgg                                 36


<210>  58
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1001179 (CAT) BIP primer


<220>
<221>  misc_feature
<222>  (1)..(2)
<223>  Nucleotides not matching with reference genome

<220>
<221>  misc_feature
<222>  (20)..(21)
<223>  Junction of first and second specific sequences

<400>  58
tacgggcacc ccgggccggc ggaagggtcc cgctct                                 36


<210>  59
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: rs1001179 (CAT) LoopF primer

<400>  59
cccggttatc agcatccttc a                                                 21


<210>  60
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 N gene F3 primer

<400>  60
tggctactac cgaagagct                                                    19


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 N gene B3 primer

<400>  61
tgcagcattg ttagcaggat                                                   20


<210>  62
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 N gene FIP primer


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  Junction of first and second specific sequences

<400>  62
tctggcccag ttcctaggta gtgacgaatt cgtggtggtg a                           41


<210>  63
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 N gene BIP primer


<220>
<221>  misc_feature
<222>  (22)..(23)
<223>  Junction of first and second specific sequences

<400>  63
agacggcatc atatgggttg cagcgggtgc caatgtgatc                             40


<210>  64
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 N gene LoopF primer

<400>  64
tggactgaga tctttcattt taccg                                             25


<210>  65
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 ORF1ab F3 primer

<400>  65
gaaaagaaaa agcttgatgg c                                                 21


<210>  66
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 ORF1ab B3 primer

<400>  66
ggtaagtaac cacaagtagt g                                                 21


<210>  67
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 ORF1ab FIP primer


<220>
<221>  misc_feature
<222>  (24)..(25)
<223>  Junction of first and second specific sequences

<400>  67
agagttgaaa ggcacatttg gttgtttatg ggtagaattc gatctg                      46


<210>  68
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 ORF1ab BIP primer


<220>
<221>  misc_feature
<222>  (23)..(24)
<223>  Junction of first and second specific sequences

<400>  68
cagacgggcg attttgttaa agccaccttc tttagtcaaa ttctca                      46


<210>  69
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: COVID19 ORF1ab LoopF primer

<400>  69
tggtgacgca actggataga                                                   20


