                         SEQUENCE LISTING

<110>  BLISS BIOPHARMACEUTICAL (HANGZHOU) CO., LTD.
 
<120>  ANTI-CD276 ANTIBODY, ANTIBODY-DRUG CONJUGATE, AND USE THEREOF

<130>  P21408941C

<150>  PCT/CN2020/130409
<151>  2020-11-20

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH of 27B4H4L3 and BH73

<400>  1

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Tyr Pro Gly Asp Gly Gly Ser Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Asn Tyr Ile Ser Phe Pro Met Tyr Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  2
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL of 27B4H4L3 and BH73

<400>  2

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Lys Ala Ser Arg Gly Val Ser Thr Ala 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln His Tyr Asp Thr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  3
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy Chain of 27B4H4L3

<400>  3

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Tyr Pro Gly Asp Gly Gly Ser Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Asn Tyr Ile Ser Phe Pro Met Tyr Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Arg Val Glu Pro Lys Ser Cys Asp 
    210                 215                 220                 


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Val Gly Gly 
225                 230                 235                 240 


Pro Ser Val Phe Leu Leu Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
                245                 250                 255     


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
            260                 265                 270         


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
        275                 280                 285             


Asn Ala Lys Thr Lys Pro Pro Glu Glu Gln Tyr Asn Ser Thr Leu Arg 
    290                 295                 300                 


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
305                 310                 315                 320 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
                325                 330                 335     


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
            340                 345                 350         


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
        355                 360                 365             


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
    370                 375                 380                 


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Leu Val 
385                 390                 395                 400 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
                405                 410                 415     


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
            420                 425                 430         


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
        435                 440                 445             


Gly Lys 
    450 


<210>  4
<211>  453
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy Chain of BH73

<400>  4

Glu Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Glu 
1               5                   10                  15      


Ser Leu Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Thr Tyr 
            20                  25                  30          


Trp Ile Gly Trp Val Arg Gln Met Pro Gly Lys Gly Leu Glu Trp Met 
        35                  40                  45              


Gly Ile Ile Tyr Pro Gly Asp Gly Gly Ser Arg Tyr Ser Pro Ser Phe 
    50                  55                  60                  


Gln Gly Gln Val Thr Ile Ser Ala Asp Lys Ser Ile Ser Thr Ala Tyr 
65                  70                  75                  80  


Leu Gln Trp Ser Ser Leu Lys Ala Ser Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Gly Gly Asn Tyr Ile Ser Phe Pro Met Tyr Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val 
        115                 120                 125             


Phe Pro Leu Ala Pro Cys Ser Arg Ser Thr Ser Glu Ser Thr Ala Ala 
    130                 135                 140                 


Leu Gly Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser 
145                 150                 155                 160 


Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val 
                165                 170                 175     


Leu Gln Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro 
            180                 185                 190         


Ser Ser Asn Phe Gly Thr Gln Thr Tyr Thr Cys Asn Val Asp His Lys 
        195                 200                 205             


Pro Ser Asn Thr Lys Val Asp Lys Thr Val Glu Pro Pro Lys Ser Asp 
    210                 215                 220                 


Cys Lys Thr Lys Thr Val Glu Cys Pro Pro Cys Pro Ala Pro Glu Leu 
225                 230                 235                 240 


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
                245                 250                 255     


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
            260                 265                 270         


Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val 
        275                 280                 285             


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser 
    290                 295                 300                 


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
305                 310                 315                 320 


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala 
                325                 330                 335     


Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
            340                 345                 350         


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
        355                 360                 365             


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
    370                 375                 380                 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
385                 390                 395                 400 


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
                405                 410                 415     


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
            420                 425                 430         


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
        435                 440                 445             


Leu Ser Pro Gly Lys 
    450             


<210>  5
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL-CL (Human Kappa) of 27B4H4L3 and BH73

<400>  5

Glu Ile Val Met Thr Gln Ser Pro Ala Thr Leu Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Arg Ala Thr Leu Ser Cys Lys Ala Ser Arg Gly Val Ser Thr Ala 
            20                  25                  30          


Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile 
        35                  40                  45              


Tyr Trp Ala Ser Thr Arg Ala Thr Gly Ile Pro Ala Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Ser 
65                  70                  75                  80  


Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln His Tyr Asp Thr Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala 
            100                 105                 110         


Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly 
        115                 120                 125             


Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala 
    130                 135                 140                 


Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln 
145                 150                 155                 160 


Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser 
                165                 170                 175     


Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr 
            180                 185                 190         


Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser 
        195                 200                 205             


Phe Asn Arg Gly Glu Cys 
    210                 


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy Chain CDR1 of BH73 (and 27B4H4L3)

<400>  6

Gly Tyr Thr Phe Thr Thr Tyr Trp 
1               5               


<210>  7
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy Chain CDR2 of BH73 (and 27B4H4L3)

<400>  7

Ile Tyr Pro Gly Asp Gly Gly Ser 
1               5               


<210>  8
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Heavy Chain CDR3 of BH73 (and 27B4H4L3)

<400>  8

Ala Arg Gly Gly Asn Tyr Ile Ser Phe Pro Met Tyr Tyr 
1               5                   10              


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light Chain CDR1 of BH73 (and 27B4H4L3)

<400>  9

Arg Gly Val Ser Thr Ala 
1               5       


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Light Chain CDR3 of BH73 (and 27B4H4L3)

<400>  10

Gln Gln His Tyr Asp Thr Pro Tyr Thr 
1               5                   


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of 27B4H4L3

<400>  11

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  12
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of BH73

<400>  12

Glu Pro Pro Lys Ser Asp Cys Lys Thr Lys Thr Val Glu Cys Pro Pro 
1               5                   10                  15      


Cys Pro 
        


<210>  13
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  13

Cys Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10  


<210>  14
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  14

Asp Lys Thr Cys His Thr Cys Pro Pro Cys Pro 
1               5                   10      


<210>  15
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  15

Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  16

Glu Pro Lys Ser Asp Cys Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  17

Glu Pro Lys Ser Asp Lys Cys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  18
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  18

Glu Pro Lys Ser Asp Cys Lys Thr His Thr Val Glu Cys Pro Pro Cys 
1               5                   10                  15      


Pro 
    


<210>  19
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  19

Glu Pro Lys Ser Asp Cys Lys Thr Val Glu Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  20

Glu Pro Lys Ser Asp Lys Cys Thr His Thr Val Glu Cys Pro Pro Cys 
1               5                   10                  15      


Pro 
    


<210>  21
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  21

Glu Pro Pro Lys Ser Cys Asp Lys Thr His Thr Val Glu Cys Pro Pro 
1               5                   10                  15      


Cys Pro 
        


<210>  22
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  22

Glu Pro Pro Lys Ser Asp Cys Lys Thr His Thr Val Glu Cys Pro Pro 
1               5                   10                  15      


Cys Pro 
        


<210>  23
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  23

Glu Pro Pro Pro Lys Ser Cys Asp Lys Thr His Thr Val Glu Cys Pro 
1               5                   10                  15      


Pro Cys Pro 
            


<210>  24
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hinge Sequence of Heavy Chain of Other Examples

<400>  24

Glu Pro Pro Pro Pro Lys Ser Cys Asp Lys Thr His Thr Val Glu Cys 
1               5                   10                  15      


Pro Pro Cys Pro 
            20  


