                         SEQUENCE LISTING

<110>  Massachusetts Institute of Technology
 
<120>  Engineered Chimeric Antimicrobial Agents against Gram-Positive 
       Bacteria

<130>  SP103343WO

<150>  SG10202011321V
<151>  2020-11-13

<160>  20    

<170>  PatentIn version 3.5

<210>  1
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyV12 insert forward primer

<400>  1
ctgaacggtg gttctacccc                                                   20


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyV12 insert reverse primer

<400>  2
tttgaaggta ccccacgctt c                                                 21


<210>  3
<211>  47
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyV12 P10N-V12C forward primer

<400>  3
cgtccgccgt acgaaaaaga taccccgctg aacggtggtt ctacccc                     47


<210>  4
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PlyV12 P10N-V12C reverse primer

<400>  4
ggatcctcaa tggtggtgat gatggtgcgc tttgaaggta ccccacgctt c                51


<210>  5
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysEF-P10 insert forward primer

<400>  5
gcgccgaaac cgccgg                                                       16


<210>  6
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysEF-P10 insert reverse primer

<400>  6
cgcaactttg aactgcgggt gagacag                                           27


<210>  7
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysEF-P10 V12N-P10C forward primer

<400>  7
gtatagcatg ggttggtacg tttatcgtct ggcgccgaaa ccgccg                      46


<210>  8
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LysEF-P10 V12N-P10C reverse primer

<400>  8
ggatcctcaa tggtggtgat gatggtgcgc aactttgaac tgcgggtgag aca              53


<210>  9
<211>  338
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin LKCHAPV12 amino acid sequence

<400>  9

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Ser Ser Gly Leu Asn Gly Gly Ser Thr Pro 
                165                 170                 175     


Pro Lys Pro Asn Thr Lys Lys Val Lys Val Leu Lys His Ala Thr Asn 
            180                 185                 190         


Trp Ser Pro Ser Ser Lys Gly Ala Lys Met Ala Ser Phe Val Lys Gly 
        195                 200                 205             


Gly Thr Phe Glu Val Lys Gln Gln Arg Pro Ile Ser Tyr Ser Tyr Ser 
    210                 215                 220                 


Asn Gln Glu Tyr Leu Ile Val Asn Lys Gly Thr Val Leu Gly Trp Val 
225                 230                 235                 240 


Leu Ser Gln Asp Ile Glu Gly Gly Tyr Gly Ser Asp Arg Val Gly Gly 
                245                 250                 255     


Ser Lys Pro Lys Leu Pro Ala Gly Phe Thr Lys Glu Glu Ala Thr Phe 
            260                 265                 270         


Ile Asn Gly Asn Ala Pro Ile Thr Thr Arg Lys Asn Lys Pro Ser Leu 
        275                 280                 285             


Ser Ser Gln Thr Ala Thr Pro Leu Tyr Pro Gly Gln Ser Val Arg Tyr 
    290                 295                 300                 


Leu Gly Trp Lys Ser Ala Glu Gly Tyr Ile Trp Ile Tyr Ala Thr Asp 
305                 310                 315                 320 


Gly Arg Tyr Ile Pro Val Arg Pro Val Gly Lys Glu Ala Trp Gly Thr 
                325                 330                 335     


Phe Lys 
        


<210>  10
<211>  1014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin LKCHAPV12 nucleotide sequence

<400>  10
atggctaaaa cccaggctga aattaacaaa cgtctggatg cgtacgcaaa aggtactgtt       60

gattccccgt atcgtgttaa aaaagcgacc agctacgatc cgtccttcgg tgttatggaa      120

gcgggtgcga tcgatgcaga cggctactac cacgcacagt gtcaggatct gatcaccgat      180

tatgttctgt ggctgaccga taacaaagtt cgtacctggg gtaacgcgaa agatcagatc      240

aaacagtctt acggcaccgg tttcaaaatc catgaaaaca aaccgagcac cgttccgaaa      300

aaaggctgga ttgcggtttt tacttctggc tcttacgaac agtggggtca catcggtatc      360

gtttatgatg gtggtaacac cagcaccttc accattctgg aacagaactg gaacggttat      420

gcgaacaaaa aaccgaccaa acgtgttgat aactattatg gtctgaccca cttcattgaa      480

atcccggtta aagcgggctc ttctggtctg aacggtggta gcaccccgcc gaaaccgaac      540

accaaaaaag ttaaagttct gaaacatgca accaactgga gcccgtctag caaaggcgcg      600

aaaatggcat ccttcgttaa aggcggcacc tttgaagtta aacagcagcg tccgatcagc      660

tactcttata gcaaccagga atacctgatc gttaacaaag gtactgttct gggctgggtt      720

ctgagccagg atattgaagg tggttacggt tccgatcgtg ttggtggttc taaaccgaaa      780

ctgccggcgg gtttcaccaa agaagaagcg accttcatta acggtaacgc gccgatcact      840

acccgtaaaa ataaaccgag cctgagttct cagaccgcga ccccgctgta tccgggtcag      900

tctgttcgtt atctgggctg gaaatctgcg gaaggttaca tctggatcta cgcgaccgat      960

ggtcgttata tcccggttcg tccggttggt aaagaagcgt ggggcacctt caaa           1014


<210>  11
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin P10N-V12C amino acid sequence

<400>  11

Met Val Lys Val Asn Asp Val Val Ser Tyr Leu Asn Ser Arg Val Gly 
1               5                   10                  15      


Thr Gly Ile Asp Met Asp Gly Ala Tyr Gly Phe Gln Cys Ala Asp Leu 
            20                  25                  30          


Ala Gln Ala Ile Thr Tyr Asn Phe Phe Gly Trp Trp Phe Tyr Gly Asn 
        35                  40                  45              


Ala Ile Ala Leu Ala Ser Gln Pro Ile Pro Asn Gly Phe Glu Arg Ile 
    50                  55                  60                  


Arg Val Thr Asp Ala Thr Gln Ile Lys Ala Gly Asp Ile Val Val Trp 
65                  70                  75                  80  


Ser Glu His Glu Tyr Ala Gln Tyr Gly His Val Ala Ile Ala Ala Lys 
                85                  90                  95      


Asp Gly Tyr Ser Asp Gln Thr Phe Glu Asn Tyr Ala Gln Asn Trp Leu 
            100                 105                 110         


Asn Ala Ser Leu Thr Val Gly Ser Pro Ile Ala Leu Val Arg Thr Asn 
        115                 120                 125             


Met Tyr Gly Val Gly Tyr Val Ile Arg Pro Pro Tyr Glu Lys Asp Thr 
    130                 135                 140                 


Pro Leu Asn Gly Gly Ser Thr Pro Pro Lys Pro Asn Thr Lys Lys Val 
145                 150                 155                 160 


Lys Val Leu Lys His Ala Thr Asn Trp Ser Pro Ser Ser Lys Gly Ala 
                165                 170                 175     


Lys Met Ala Ser Phe Val Lys Gly Gly Thr Phe Glu Val Lys Gln Gln 
            180                 185                 190         


Arg Pro Ile Ser Tyr Ser Tyr Ser Asn Gln Glu Tyr Leu Ile Val Asn 
        195                 200                 205             


Lys Gly Thr Val Leu Gly Trp Val Leu Ser Gln Asp Ile Glu Gly Gly 
    210                 215                 220                 


Tyr Gly Ser Asp Arg Val Gly Gly Ser Lys Pro Lys Leu Pro Ala Gly 
225                 230                 235                 240 


Phe Thr Lys Glu Glu Ala Thr Phe Ile Asn Gly Asn Ala Pro Ile Thr 
                245                 250                 255     


Thr Arg Lys Asn Lys Pro Ser Leu Ser Ser Gln Thr Ala Ile Pro Leu 
            260                 265                 270         


Tyr Pro Gly Gln Ser Val Arg Tyr Leu Gly Trp Lys Ser Ala Glu Gly 
        275                 280                 285             


Tyr Ile Trp Ile Tyr Ala Thr Asp Gly Arg Tyr Ile Pro Val Arg Pro 
    290                 295                 300                 


Val Gly Lys Glu Ala Trp Gly Thr Phe Lys Ala His His His His His 
305                 310                 315                 320 


His 
    


<210>  12
<211>  963
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin P10N-V12C nucleotide sequence

<400>  12
atggttaaag ttaacgatgt tgttagctac ctgaactctc gtgttggtac cggtatcgat       60

atggatggtg cgtacggttt ccagtgcgcg gatctggcgc aggcgatcac ctacaacttc      120

ttcggttggt ggttctacgg caacgcgatc gcactggcgt ctcagccgat cccgaacggc      180

ttcgaacgta tccgtgttac cgatgcgacc cagatcaaag cgggcgatat cgttgtttgg      240

agcgaacacg aatacgcgca gtacggtcac gttgcgatcg cggcgaaaga tggctacagc      300

gatcagacct tcgaaaacta cgcgcagaac tggctgaacg cgagcctgac cgttggtagc      360

ccgatcgcgc tggttcgtac caacatgtac ggcgttggtt acgttatccg tccgccgtac      420

gaaaaagata ccccgctgaa cggtggttct accccgccta aaccgaacac taaaaaagtt      480

aaagttctga aacatgcaac caactggtct ccgagctcta aaggtgcgaa aatggctagc      540

ttcgttaaag gcggtacttt cgaagttaaa cagcagcgtc cgattagcta ctcttactct      600

aaccaggaat acctgattgt taacaaaggt accgttctgg gttgggttct gagccaggat      660

attgaaggtg gttacggctc tgatcgtgtt ggcggtagca aaccgaaact gccggctggt      720

ttcaccaaag aagaagcgac cttcatcaac ggtaacgctc cgatcaccac ccgtaaaaac      780

aaaccgtctc tgtctagcca gaccgcgatc ccgctgtatc cgggtcagag cgttcgttac      840

ctgggctgga aatctgctga aggttacatc tggatctacg cgaccgatgg tcgttacatc      900

ccggttcgtc cggttggtaa agaagcgtgg ggtaccttca aagcgcacca tcatcaccac      960

cat                                                                    963


<210>  13
<211>  242
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin V12NM3 amino acid sequence

<400>  13

Met Ser Asn Ile Asn Met Glu Thr Ala Ile Ala Asn Met Tyr Ala Leu 
1               5                   10                  15      


Lys Ala Arg Gly Ile Thr Tyr Ser Met Asn Tyr Ser Arg Thr Gly Ala 
            20                  25                  30          


Asp Gly Thr Gly Asp Cys Ser Gly Thr Val Tyr Asp Ser Leu Arg Lys 
        35                  40                  45              


Ala Gly Ala Ser Asp Ala Gly Trp Val Leu Asn Thr Asp Ser Met His 
    50                  55                  60                  


Ser Trp Leu Glu Lys Asn Gly Phe Lys Leu Ile Ala Gln Asn Lys Glu 
65                  70                  75                  80  


Trp Ser Ala Lys Arg Gly Asp Val Val Ile Phe Gly Lys Lys Gly Ala 
                85                  90                  95      


Ser Gly Gly Ser Ala Gly His Val Val Ile Phe Ile Ser Ser Thr Gln 
            100                 105                 110         


Ile Ile His Cys Thr Trp Lys Ser Ala Thr Ala Asn Gly Val Tyr Val 
        115                 120                 125             


Asp Asn Glu Ala Thr Thr Cys Pro Tyr Ser Met Gly Trp Tyr Val Tyr 
    130                 135                 140                 


Arg Gly Ser Ser Gly Lys Ser Ala Ser Lys Ile Thr Val Gly Ser Lys 
145                 150                 155                 160 


Ala Pro Tyr Asn Leu Lys Trp Ser Lys Gly Ala Tyr Phe Asn Ala Lys 
                165                 170                 175     


Ile Asp Gly Leu Gly Ala Thr Ser Ala Thr Arg Tyr Gly Asp Asn Arg 
            180                 185                 190         


Thr Asn Tyr Arg Phe Asp Val Gly Gln Ala Val Tyr Ala Pro Gly Thr 
        195                 200                 205             


Leu Ile Tyr Val Phe Glu Ile Ile Asp Gly Trp Cys Arg Ile Tyr Trp 
    210                 215                 220                 


Asn Asn His Asn Glu Trp Ile Trp His Glu Arg Leu Ile Val Lys Glu 
225                 230                 235                 240 


Val Phe 
        


<210>  14
<211>  726
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin V12NM3 nucleotide sequence

<400>  14
atgtctaaca ttaacatgga aaccgcgatc gctaacatgt atgctctgaa agcgcgtggt       60

atcacctact ctatgaacta cagccgtacc ggtgctgatg gcaccggtga ttgttctggc      120

accgtttacg attctctgcg taaagcaggt gcttctgatg cgggttgggt tctgaacacc      180

gatagcatgc acagctggct ggagaaaaac ggcttcaaac tgattgctca gaacaaagaa      240

tggtctgcaa aacgtggtga tgttgttatc tttggtaaaa aaggtgcatc tggtggcagc      300

gcgggtcacg ttgttatctt catcagctcc acccagatca ttcattgcac ctggaaaagc      360

gctaccgcga acggtgttta cgttgataac gaagctacca cctgtccgta ctctatgggt      420

tggtacgttt accgtggctc ttctggtaaa tctgcgagca aaatcaccgt tggttctaaa      480

gcgccgtaca acctgaaatg gtctaaaggt gcgtacttca acgcaaaaat cgatggtctg      540

ggtgcgacct ctgcgacccg ttacggtgat aaccgtacca actaccgttt cgatgttggt      600

caggcggttt acgcgccggg caccctgatc tacgttttcg aaatcatcga tggttggtgc      660

cgtatctatt ggaacaacca caacgaatgg atctggcacg aacgtctgat cgttaaagaa      720

gttttc                                                                 726


<210>  15
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin LKNM3 amino acid sequence

<400>  15

Met Ala Lys Thr Gln Ala Glu Ile Asn Lys Arg Leu Asp Ala Tyr Ala 
1               5                   10                  15      


Lys Gly Thr Val Asp Ser Pro Tyr Arg Val Lys Lys Ala Thr Ser Tyr 
            20                  25                  30          


Asp Pro Ser Phe Gly Val Met Glu Ala Gly Ala Ile Asp Ala Asp Gly 
        35                  40                  45              


Tyr Tyr His Ala Gln Cys Gln Asp Leu Ile Thr Asp Tyr Val Leu Trp 
    50                  55                  60                  


Leu Thr Asp Asn Lys Val Arg Thr Trp Gly Asn Ala Lys Asp Gln Ile 
65                  70                  75                  80  


Lys Gln Ser Tyr Gly Thr Gly Phe Lys Ile His Glu Asn Lys Pro Ser 
                85                  90                  95      


Thr Val Pro Lys Lys Gly Trp Ile Ala Val Phe Thr Ser Gly Ser Tyr 
            100                 105                 110         


Glu Gln Trp Gly His Ile Gly Ile Val Tyr Asp Gly Gly Asn Thr Ser 
        115                 120                 125             


Thr Phe Thr Ile Leu Glu Gln Asn Trp Asn Gly Tyr Ala Asn Lys Lys 
    130                 135                 140                 


Pro Thr Lys Arg Val Asp Asn Tyr Tyr Gly Leu Thr His Phe Ile Glu 
145                 150                 155                 160 


Ile Pro Val Lys Ala Gly Ser Ser Gly Lys Ser Ala Ser Lys Ile Thr 
                165                 170                 175     


Val Gly Ser Lys Ala Pro Tyr Asn Leu Lys Trp Ser Lys Gly Ala Tyr 
            180                 185                 190         


Phe Asn Ala Lys Ile Asp Gly Leu Gly Ala Thr Ser Ala Thr Arg Tyr 
        195                 200                 205             


Gly Asp Asn Arg Thr Asn Tyr Arg Phe Asp Val Gly Gln Ala Val Tyr 
    210                 215                 220                 


Ala Pro Gly Thr Leu Ile Tyr Val Phe Glu Ile Ile Asp Gly Trp Cys 
225                 230                 235                 240 


Arg Ile Tyr Trp Asn Asn His Asn Glu Trp Ile Trp His Glu Arg Leu 
                245                 250                 255     


Ile Val Lys Glu Val Phe 
            260         


<210>  16
<211>  786
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin LKNM3 nucleotide sequence

<400>  16
atggctaaaa cccaggctga aattaacaaa cgtctggatg cgtacgcaaa aggtactgtt       60

gattccccgt atcgtgttaa aaaagcgacc agctacgatc cgtccttcgg tgttatggaa      120

gcgggtgcga tcgatgcaga cggctactac cacgcacagt gtcaggatct gatcaccgat      180

tatgttctgt ggctgaccga taacaaagtt cgtacctggg gtaacgcgaa agatcagatc      240

aaacagtctt acggcaccgg tttcaaaatc catgaaaaca aaccgagcac cgttccgaaa      300

aaaggctgga ttgcggtttt tacttctggc tcttacgaac agtggggtca catcggtatc      360

gtttatgatg gtggtaacac cagcaccttc accattctgg aacagaactg gaacggttat      420

gcgaacaaaa aaccgaccaa acgtgttgat aactattatg gtctgaccca cttcattgaa      480

atcccggtta aagcgggttc ttctggtaaa tctgcgagca aaatcaccgt tggttctaaa      540

gcgccgtaca acctgaaatg gtctaaaggt gcgtacttca acgcaaaaat cgatggtctg      600

ggtgcgacct ctgcgacccg ttacggtgat aaccgtacca actaccgttt cgatgttggt      660

caggcggttt acgcgccggg caccctgatc tacgttttcg aaatcatcga tggttggtgc      720

cgtatctatt ggaacaacca caacgaatgg atctggcacg aacgtctgat cgttaaagaa      780

gttttc                                                                 786


<210>  17
<211>  321
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin PlyV12 amino acid sequence

<400>  17

Met Ser Asn Ile Asn Met Glu Thr Ala Ile Ala Asn Met Tyr Ala Leu 
1               5                   10                  15      


Lys Ala Arg Gly Ile Thr Tyr Ser Met Asn Tyr Ser Arg Thr Gly Ala 
            20                  25                  30          


Asp Gly Thr Gly Asp Cys Ser Gly Thr Val Tyr Asp Ser Leu Arg Lys 
        35                  40                  45              


Ala Gly Ala Ser Asp Ala Gly Trp Val Leu Asn Thr Asp Ser Met His 
    50                  55                  60                  


Ser Trp Leu Glu Lys Asn Gly Phe Lys Leu Ile Ala Gln Asn Lys Glu 
65                  70                  75                  80  


Trp Ser Ala Lys Arg Gly Asp Val Val Ile Phe Gly Lys Lys Gly Ala 
                85                  90                  95      


Ser Gly Gly Ser Ala Gly His Val Val Ile Phe Ile Ser Ser Thr Gln 
            100                 105                 110         


Ile Ile His Cys Thr Trp Lys Ser Ala Thr Ala Asn Gly Val Tyr Val 
        115                 120                 125             


Asp Asn Glu Ala Thr Thr Cys Pro Tyr Ser Met Gly Trp Tyr Val Tyr 
    130                 135                 140                 


Arg Leu Asn Gly Gly Ser Thr Pro Pro Lys Pro Asn Thr Lys Lys Val 
145                 150                 155                 160 


Lys Val Leu Lys His Ala Thr Asn Trp Ser Pro Ser Ser Lys Gly Ala 
                165                 170                 175     


Lys Met Ala Ser Phe Val Lys Gly Gly Thr Phe Glu Val Lys Gln Gln 
            180                 185                 190         


Arg Pro Ile Ser Tyr Ser Tyr Ser Asn Gln Glu Tyr Leu Ile Val Asn 
        195                 200                 205             


Lys Gly Thr Val Leu Gly Trp Val Leu Ser Gln Asp Ile Glu Gly Gly 
    210                 215                 220                 


Tyr Gly Ser Asp Arg Val Gly Gly Ser Lys Pro Lys Leu Pro Ala Gly 
225                 230                 235                 240 


Phe Thr Lys Glu Glu Ala Thr Phe Ile Asn Gly Asn Ala Pro Ile Thr 
                245                 250                 255     


Thr Arg Lys Asn Lys Pro Ser Leu Ser Ser Gln Thr Ala Thr Pro Leu 
            260                 265                 270         


Tyr Pro Gly Gln Ser Val Arg Tyr Leu Gly Trp Lys Ser Ala Glu Gly 
        275                 280                 285             


Tyr Ile Trp Ile Tyr Ala Thr Asp Gly Arg Tyr Ile Pro Val Arg Pro 
    290                 295                 300                 


Val Gly Lys Glu Ala Trp Gly Thr Phe Lys Ala His His His His His 
305                 310                 315                 320 


His 
    


<210>  18
<211>  963
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin PlyV12 nucleotide sequence

<400>  18
atgtctaaca tcaacatgga aaccgcaatc gctaacatgt acgcgctgaa agctcgtggt       60

attacctata gcatgaacta tagccgtacc ggtgctgatg gtaccggtga ttgctctggc      120

accgtgtacg atagcctgcg taaagcgggc gctagcgatg ctggctgggt tctgaacacc      180

gattctatgc attcctggct ggaaaagaac ggctttaaac tgatcgcaca gaacaaagaa      240

tggtctgcta aacgtggtga cgttgttatt ttcggtaaaa aaggtgctag cggcggtagc      300

gctggccacg ttgttatctt catcagcagc acccagatta tccactgtac ctggaaatct      360

gcgaccgcga acggtgttta cgttgataac gaagctacca cctgcccgta tagcatgggt      420

tggtacgttt atcgtctgaa cggtggttct accccgccta aaccgaacac taaaaaagtt      480

aaagttctga aacatgcaac caactggtct ccgagctcta aaggtgcgaa aatggctagc      540

ttcgttaaag gcggtacttt cgaagttaaa cagcagcgtc cgattagcta ctcttactct      600

aaccaggaat acctgattgt taacaaaggt accgttctgg gttgggttct gagccaggat      660

attgaaggtg gttacggctc tgatcgtgtt ggcggtagca aaccgaaact gccggctggt      720

ttcaccaaag aagaagcgac cttcatcaac ggtaacgctc cgatcaccac ccgtaaaaac      780

aaaccgtctc tgtctagcca gaccgcgacc ccgctgtatc cgggtcagag cgttcgttac      840

ctgggctgga aatctgctga aggttacatc tggatctacg cgaccgatgg tcgttacatc      900

ccggttcgtc cggttggtaa agaagcgtgg ggtaccttca aagcgcacca tcatcaccac      960

cat                                                                    963


<210>  19
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Lysin LysEF-P10 amino acid sequence

<400>  19

Met His His His His His His Ser Ser Gly Val Asp Leu Gly Thr Glu 
1               5                   10                  15      


Asn Leu Tyr Phe Gln Ser Met Val Lys Val Asn Asp Val Val Ser Tyr 
            20                  25                  30          


Leu Asn Ser Arg Val Gly Thr Gly Ile Asp Met Asp Gly Ala Tyr Gly 
        35                  40                  45              


Phe Gln Cys Ala Asp Leu Ala Gln Ala Ile Thr Tyr Asn Phe Phe Gly 
    50                  55                  60                  


Trp Trp Phe Tyr Gly Asn Ala Ile Ala Leu Ala Ser Gln Pro Ile Pro 
65                  70                  75                  80  


Asn Gly Phe Glu Arg Ile Arg Val Thr Asp Ala Thr Gln Ile Lys Ala 
                85                  90                  95      


Gly Asp Ile Val Val Trp Ser Glu His Glu Tyr Ala Gln Tyr Gly His 
            100                 105                 110         


Val Ala Ile Ala Ala Lys Asp Gly Tyr Ser Asp Gln Thr Phe Glu Asn 
        115                 120                 125             


Tyr Ala Gln Asn Trp Leu Asn Ala Ser Leu Thr Val Gly Ser Pro Ile 
    130                 135                 140                 


Ala Leu Val Arg Thr Asn Met Tyr Gly Val Gly Tyr Val Ile Arg Pro 
145                 150                 155                 160 


Pro Tyr Glu Lys Asp Thr Pro Ala Pro Lys Pro Pro Ala Pro Lys Pro 
                165                 170                 175     


Ala Lys Pro Asn Leu Pro Thr Pro Lys Gly Asp Asp Thr Met Leu Ala 
            180                 185                 190         


Ile Tyr Tyr Lys His Leu Lys Asn Gly Asn Val Glu Gln Trp Leu Leu 
        195                 200                 205             


Ile Asn Gly Lys Arg Val Tyr Leu Pro Thr Gln Thr Trp Val Asn Glu 
    210                 215                 220                 


Ala Asn Ala Leu Ile Lys Ala Tyr Gly Gly Thr Lys Glu Val Val Gln 
225                 230                 235                 240 


Tyr Asn His Asp Asn Phe Gly Leu Lys Leu Leu Glu Leu Ser His Pro 
                245                 250                 255     


Gln Phe Lys Val 
            260 


<210>  20
<211>  780
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lysin LysEF-P10 nucleotide sequence

<400>  20
atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc       60

caatccatgg ttaaagttaa cgatgttgtt agctacctga actctcgtgt tggtaccggt      120

atcgatatgg atggtgcgta cggtttccag tgcgcggatc tggcgcaggc gatcacctac      180

aacttcttcg gttggtggtt ctacggcaac gcgatcgcac tggcgtctca gccgatcccg      240

aacggcttcg aacgtatccg tgttaccgat gcgacccaga tcaaagcggg cgatatcgtt      300

gtttggagcg aacacgaata cgcgcagtac ggtcacgttg cgatcgcggc gaaagatggc      360

tacagcgatc agaccttcga aaactacgcg cagaactggc tgaacgcgag cctgaccgtt      420

ggtagcccga tcgcgctggt tcgtaccaac atgtacggcg ttggttacgt tatccgtccg      480

ccgtacgaaa aagatacccc ggcgccgaaa ccgccggctc cgaaaccggc taaaccgaac      540

ctgccgaccc cgaaaggtga tgataccatg ctggctatct attacaaaca tctgaaaaac      600

ggtaacgttg aacagtggct gctgattaac ggtaaacgcg tttacctgcc gacccagacc      660

tgggttaacg aagcgaacgc tctgattaaa gcgtatggtg gcaccaaaga agttgttcag      720

tataaccatg ataacttcgg cctgaaactg ctggaactgt ctcacccgca gttcaaagtt      780


