                         SEQUENCE LISTING

<110>  University of Maryland, College Park
 
<120>  MODIFIED SECRETED HEPATITIS C VIRUS (HCV) E1E2 GLYCOPROTEINS AND 
       METHODS OF USE THEREOF

<130>  36429.0030P1

<150>  63/113,180
<151>  2020-11-12

<150>  63/260,475
<151>  2021-08-20

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; HCV E1 polypeptide

<400>  1

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala 
145                 150                 155             


<210>  2
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; HCV E2 polypeptide

<400>  2

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
65                  70                  75                  80  


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
            180                 185                 190         


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile 
                325                 330     


<210>  3
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; coiled-coil domain

<400>  3

Ala Ala Glu Asp Leu Leu Glu Leu Ala His Thr Ile Leu Lys Thr Ala 
1               5                   10                  15      


Arg Asn Gln Leu Arg Thr Met Glu Ile Leu Arg Lys Glu Arg 
            20                  25                  30  


<210>  4
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; coiled-coil domain

<400>  4

Ala Asp Glu Arg Arg Lys Ala Lys Glu Leu Leu Lys Glu Ala Glu Glu 
1               5                   10                  15      


Ile Trp Lys Arg Ile Asn Glu Leu Ala Glu Arg Glu Thr Lys 
            20                  25                  30  


<210>  5
<211>  587
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified HCV E1E2 glycoproteins

<400>  5

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Pro Gly 
145                 150                 155                 160 


Gly Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln 
                165                 170                 175     


Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala 
            180                 185                 190         


Gln Leu Lys Gln Lys Val Met Asn Tyr Arg Arg Arg Arg Arg Arg Glu 
        195                 200                 205             


Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu Val 
    210                 215                 220                 


Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn Thr 
225                 230                 235                 240 


Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu Ser 
                245                 250                 255     


Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe Asn 
            260                 265                 270         


Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr Asp 
        275                 280                 285             


Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly Leu 
    290                 295                 300                 


Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly Ile 
305                 310                 315                 320 


Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro Ser 
                325                 330                 335     


Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr Ser 
            340                 345                 350         


Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg Pro 
        355                 360                 365             


Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly Phe 
    370                 375                 380                 


Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly Asn 
385                 390                 395                 400 


Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu Ala 
                405                 410                 415     


Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys Met 
            420                 425                 430         


Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn Tyr 
        435                 440                 445             


Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg Leu 
    450                 455                 460                 


Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu Asp 
465                 470                 475                 480 


Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln Trp 
                485                 490                 495     


Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr Gly 
            500                 505                 510         


Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr Gly 
        515                 520                 525             


Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Pro Gly Gly Leu Thr Asp 
    530                 535                 540                 


Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Lys Lys Ser Ala Leu 
545                 550                 555                 560 


Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu Phe 
                565                 570                 575     


Ile Leu Ala Ala Tyr His His His His His His 
            580                 585         


<210>  6
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified HCV E1E2 glycoproteins

<400>  6

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln Pro Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
65                  70                  75                  80  


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
            180                 185                 190         


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile 
                325                 330     


<210>  7
<211>  331
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified HCV E2 polypeptide

<400>  7

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
65                  70                  75                  80  


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
            180                 185                 190         


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Val Arg Met Asn Val Ser Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile 
                325                 330     


<210>  8
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; c-Jun sequence

<400>  8

Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln Asn 
1               5                   10                  15      


Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala Gln 
            20                  25                  30          


Leu Lys Gln Lys Val Met Asn Tyr 
        35                  40  


<210>  9
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; c-Fos sequence

<400>  9

Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Lys Lys 
1               5                   10                  15      


Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys 
            20                  25                  30          


Leu Glu Phe Ile Leu Ala Ala Tyr 
        35                  40  


<210>  10
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; c-Jun sequence

<400>  10

Pro Gly Gly Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys 
1               5                   10                  15      


Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln 
            20                  25                  30          


Val Ala Gln Leu Lys Gln Lys Val Met Asn Tyr 
        35                  40              


<210>  11
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; c-Fos sequence

<400>  11

Pro Gly Gly Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu 
1               5                   10                  15      


Asp Lys Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu 
            20                  25                  30          


Lys Glu Lys Leu Glu Phe Ile Leu Ala Ala Tyr 
        35                  40              


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site

<400>  12

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  13
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site

<400>  13

Arg Arg Arg Arg Lys Arg 
1               5       


<210>  14
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site

<400>  14

Arg Arg Arg Lys Lys Arg 
1               5       


<210>  15
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  15

Arg Xaa Lys Arg 
1               


<210>  16
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<400>  16

Arg Xaa Arg Arg 
1               


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site

<400>  17

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


<210>  18
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; cleavage site

<400>  18

Leu Glu Val Leu Phe Gln Gly Pro 
1               5               


<210>  19
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  19

Met Gly Val Leu Leu Thr Gln Arg Thr Leu Leu Ser Leu Val Leu Ala 
1               5                   10                  15      


Leu Leu Phe Pro Ser Met Ala Ser Met 
            20                  25  


<210>  20
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  20

Met Lys Cys Leu Leu Tyr Leu Ala Phe Leu Phe Ile Gly Val Asn Cys 
1               5                   10                  15      


<210>  21
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  21

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Asp 
            20      


<210>  22
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  22

Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      


Val His Ser 
            


<210>  23
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  23

Met Arg Ala Trp Ile Phe Phe Leu Leu Cys Leu Ala Gly Arg Ala Leu 
1               5                   10                  15      


Ala 
    


<210>  24
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  24

Met Trp Trp Arg Leu Trp Trp Leu Leu Leu Leu Leu Leu Leu Leu Trp 
1               5                   10                  15      


Pro Met Val Trp Ala 
            20      


<210>  25
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  25

Met Asp Met Arg Val Pro Ala Gln Leu Leu Gly Leu Leu Leu Leu Trp 
1               5                   10                  15      


Leu Arg Gly Ala Arg Cys 
            20          


<210>  26
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  26

Met Pro Leu Leu Leu Leu Leu Pro Leu Leu Trp Ala Gly Ala Leu Ala 
1               5                   10                  15      


<210>  27
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  27

Met Asp Ala Met Lys Arg Gly Leu Cys Cys Val Leu Leu Leu Cys Gly 
1               5                   10                  15      


Ala Val Phe Val Ser Pro Ser 
            20              


<210>  28
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  28

Met Ala Phe Leu Trp Leu Leu Ser Cys Trp Ala Leu Leu Gly Thr Thr 
1               5                   10                  15      


Phe Gly 
        


<210>  29
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  29

Met Asn Leu Leu Leu Ile Leu Thr Phe Val Ala Ala Ala Val Ala 
1               5                   10                  15  


<210>  30
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  30

Met Tyr Arg Met Gln Leu Leu Ser Cys Ile Ala Leu Ser Leu Ala Leu 
1               5                   10                  15      


Val Thr Asn Ser 
            20  


<210>  31
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  31

Met Gly Val Lys Val Leu Phe Ala Leu Ile Cys Ile Ala Val Ala Glu 
1               5                   10                  15      


Ala 
    


<210>  32
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  32

Met Lys Trp Val Thr Phe Ile Ser Leu Leu Phe Ser Ser Ala Tyr Ser 
1               5                   10                  15      


<210>  33
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  33

Met Lys Thr Ile Ile Ala Leu Ser Tyr Ile Phe Cys Leu Val Leu Gly 
1               5                   10                  15      


<210>  34
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  34

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala 
            20                  


<210>  35
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; leader sequence

<400>  35

Met Lys Pro Ile Phe Leu Val Leu Leu Val Val Thr Ser Ala Tyr Ala 
1               5                   10                  15      


<210>  36
<211>  555
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; membrane bound E1/E2

<400>  36

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Gly Ala 
145                 150                 155                 160 


His Trp Gly Val Leu Ala Gly Ile Ala Tyr Phe Ser Met Val Gly Asn 
                165                 170                 175     


Trp Ala Lys Val Leu Val Val Leu Leu Leu Phe Ala Gly Val Asp Ala 
            180                 185                 190         


Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
        195                 200                 205             


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
    210                 215                 220                 


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
225                 230                 235                 240 


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe 
                245                 250                 255     


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
            260                 265                 270         


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
        275                 280                 285             


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
    290                 295                 300                 


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
305                 310                 315                 320 


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
                325                 330                 335     


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
            340                 345                 350         


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
        355                 360                 365             


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
    370                 375                 380                 


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
385                 390                 395                 400 


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
                405                 410                 415     


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
            420                 425                 430         


Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg 
        435                 440                 445             


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
    450                 455                 460                 


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
465                 470                 475                 480 


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
                485                 490                 495     


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
            500                 505                 510         


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Lys Trp Glu Tyr Val 
        515                 520                 525             


Val Leu Leu Phe Leu Leu Leu Ala Asp Ala Arg Val Cys Ser Cys Leu 
    530                 535                 540                 


Trp Met Met Leu Leu Ile Ser Gln Ala Glu Ala 
545                 550                 555 


<210>  37
<211>  530
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified E1/E2

<400>  37

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Gly Ser 
145                 150                 155                 160 


Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
                165                 170                 175     


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe Leu Arg Arg Arg Arg Arg 
            180                 185                 190         


Arg Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly 
        195                 200                 205             


Leu Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile 
    210                 215                 220                 


Asn Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn 
225                 230                 235                 240 


Glu Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys 
                245                 250                 255     


Phe Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu 
            260                 265                 270         


Thr Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser 
        275                 280                 285             


Gly Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys 
    290                 295                 300                 


Gly Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr 
305                 310                 315                 320 


Pro Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr 
                325                 330                 335     


Tyr Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr 
            340                 345                 350         


Arg Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr 
        355                 360                 365             


Gly Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val 
    370                 375                 380                 


Gly Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro 
385                 390                 395                 400 


Glu Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg 
                405                 410                 415     


Cys Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile 
            420                 425                 430         


Asn Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His 
        435                 440                 445             


Arg Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu 
    450                 455                 460                 


Glu Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr 
465                 470                 475                 480 


Gln Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser 
                485                 490                 495     


Thr Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu 
            500                 505                 510         


Tyr Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile His His His His 
        515                 520                 525             


His His 
    530 


<210>  38
<211>  561
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified E/E2

<400>  38

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Ala Ala 
145                 150                 155                 160 


Glu Asp Leu Leu Glu Leu Ala His Thr Ile Leu Lys Thr Ala Arg Asn 
                165                 170                 175     


Gln Leu Arg Thr Met Glu Ile Leu Arg Lys Glu Arg Arg Arg Arg Arg 
            180                 185                 190         


Arg Arg Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala 
        195                 200                 205             


Gly Leu Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu 
    210                 215                 220                 


Ile Asn Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys 
225                 230                 235                 240 


Asn Glu Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His 
                245                 250                 255     


Lys Phe Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg 
            260                 265                 270         


Leu Thr Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly 
        275                 280                 285             


Ser Gly Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro 
    290                 295                 300                 


Cys Gly Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe 
305                 310                 315                 320 


Thr Pro Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro 
                325                 330                 335     


Thr Tyr Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn 
            340                 345                 350         


Thr Arg Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser 
        355                 360                 365             


Thr Gly Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly 
    370                 375                 380                 


Val Gly Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His 
385                 390                 395                 400 


Pro Glu Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro 
                405                 410                 415     


Arg Cys Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr 
            420                 425                 430         


Ile Asn Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu 
        435                 440                 445             


His Arg Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp 
    450                 455                 460                 


Leu Glu Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr 
465                 470                 475                 480 


Thr Gln Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu 
                485                 490                 495     


Ser Thr Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr 
            500                 505                 510         


Leu Tyr Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Ala Asp Glu 
        515                 520                 525             


Arg Arg Lys Ala Lys Glu Leu Leu Lys Glu Ala Glu Glu Ile Trp Lys 
    530                 535                 540                 


Arg Ile Asn Glu Leu Ala Glu Arg Glu Thr Lys His His His His His 
545                 550                 555                 560 


His 
    


<210>  39
<211>  501
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified E1/E2

<400>  39

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Arg Arg 
145                 150                 155                 160 


Arg Arg Arg Arg Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr 
                165                 170                 175     


Thr Ala Gly Leu Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile 
            180                 185                 190         


Gln Leu Ile Asn Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu 
        195                 200                 205             


Asn Cys Asn Glu Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr 
    210                 215                 220                 


Gln His Lys Phe Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys 
225                 230                 235                 240 


Arg Arg Leu Thr Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala 
                245                 250                 255     


Asn Gly Ser Gly Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro 
            260                 265                 270         


Arg Pro Cys Gly Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr 
        275                 280                 285             


Cys Phe Thr Pro Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly 
    290                 295                 300                 


Ala Pro Thr Tyr Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu 
305                 310                 315                 320 


Asn Asn Thr Arg Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met 
                325                 330                 335     


Asn Ser Thr Gly Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile 
            340                 345                 350         


Gly Gly Val Gly Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg 
        355                 360                 365             


Lys His Pro Glu Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile 
    370                 375                 380                 


Thr Pro Arg Cys Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro 
385                 390                 395                 400 


Cys Thr Ile Asn Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly 
                405                 410                 415     


Val Glu His Arg Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg 
            420                 425                 430         


Cys Asp Leu Glu Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu 
        435                 440                 445             


Ser Thr Thr Gln Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro 
    450                 455                 460                 


Ala Leu Ser Thr Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val 
465                 470                 475                 480 


Gln Tyr Leu Tyr Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile His 
                485                 490                 495     


His His His His His 
            500     


<210>  40
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified E1/E2

<400>  40

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Thr His 
                165                 170                 175     


Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu Val Gly Leu 
            180                 185                 190         


Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn Thr Asn Gly 
        195                 200                 205             


Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu Ser Leu Asn 
    210                 215                 220                 


Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe Asn Ser Ser 
225                 230                 235                 240 


Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr Asp Phe Ala 
                245                 250                 255     


Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly Leu Asp Glu 
            260                 265                 270         


Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly Ile Val Pro 
        275                 280                 285             


Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro Ser Pro Val 
    290                 295                 300                 


Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr Ser Trp Gly 
305                 310                 315                 320 


Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg Pro Pro Leu 
                325                 330                 335     


Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly Phe Thr Lys 
            340                 345                 350         


Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly Asn Asn Thr 
        355                 360                 365             


Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu Ala Thr Tyr 
    370                 375                 380                 


Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys Met Val Asp 
385                 390                 395                 400 


Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn Tyr Thr Ile 
                405                 410                 415     


Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg Leu Glu Ala 
            420                 425                 430         


Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu Asp Arg Asp 
        435                 440                 445             


Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln Trp Gln Val 
    450                 455                 460                 


Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr Gly Leu Ile 
465                 470                 475                 480 


His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr Gly Val Gly 
                485                 490                 495     


Ser Ser Ile Ala Ser Trp Ala Ile His His His His His His 
            500                 505                 510 


<210>  41
<211>  510
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; modified E1/E2

<400>  41

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
65                  70                  75                  80  


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
            180                 185                 190         


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Gly Gly Gly Gly Ser 
                325                 330                 335     


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Tyr Gln Val Arg Asn Ser 
            340                 345                 350         


Ser Gly Leu Tyr His Val Thr Asn Asp Cys Pro Asn Ser Ser Ile Val 
        355                 360                 365             


Tyr Glu Ala Ala Asp Ala Ile Leu His Thr Pro Gly Cys Val Pro Cys 
    370                 375                 380                 


Val Arg Glu Gly Asn Ala Ser Arg Cys Trp Val Ala Val Thr Pro Thr 
385                 390                 395                 400 


Val Ala Thr Arg Asp Gly Lys Leu Pro Thr Thr Gln Leu Arg Arg His 
                405                 410                 415     


Ile Asp Leu Leu Val Gly Ser Ala Thr Leu Cys Ser Ala Leu Tyr Val 
            420                 425                 430         


Gly Asp Leu Cys Gly Ser Val Phe Leu Val Gly Gln Leu Phe Thr Phe 
        435                 440                 445             


Ser Pro Arg Arg His Trp Thr Thr Gln Asp Cys Asn Cys Ser Ile Tyr 
    450                 455                 460                 


Pro Gly His Ile Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn 
465                 470                 475                 480 


Trp Ser Pro Thr Ala Ala Leu Val Val Ala Gln Leu Leu Arg Ile Pro 
                485                 490                 495     


Gln Ala Ile Met Asp Met Ile Ala His His His His His His 
            500                 505                 510 


<210>  42
<211>  201
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E1-Jun

<400>  42

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Val Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Thr 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Asp 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Ala Ala Leu Val Val Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Met Asp Met Ile Ala Pro Gly 
145                 150                 155                 160 


Gly Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr Leu Lys Ala Gln 
                165                 170                 175     


Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg Glu Gln Val Ala 
            180                 185                 190         


Gln Leu Lys Gln Lys Val Met Asn Tyr 
        195                 200     


<210>  43
<211>  380
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E2-fos

<400>  43

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn Ser Thr Ala Leu Asn Cys Asn Glu 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Arg Leu Thr 
65                  70                  75                  80  


Asp Phe Ala Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Leu Asp Glu Arg Pro Tyr Cys Trp His Tyr Pro Pro Arg Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Ala Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Val Gly 
            180                 185                 190         


Asn Asn Thr Leu Leu Cys Pro Thr Asp Cys Phe Arg Lys His Pro Glu 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Met Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Val Arg Met Tyr Val Gly Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Ser Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Pro Gly Gly Leu Thr 
                325                 330                 335     


Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu Asp Lys Lys Ser Ala 
            340                 345                 350         


Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu Lys Glu Lys Leu Glu 
        355                 360                 365             


Phe Ile Leu Ala Ala Tyr His His His His His His 
    370                 375                 380 


<210>  44
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; coiled coil domain

<400>  44

Ala Ala Glu Asp Leu Leu Glu Leu Ala His Thr Ile Leu Lys Thr Ala 
1               5                   10                  15      


Arg Asn Gln Leu Arg Thr Met Glu Ile Leu Arg Lys Glu Arg 
            20                  25                  30  


<210>  45
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; coiled coil domain

<400>  45

Ala Asp Glu Arg Arg Lys Ala Lys Glu Leu Leu Lys Glu Ala Glu Glu 
1               5                   10                  15      


Ile Trp Lys Arg Ile Asn Glu Leu Ala Glu Arg Glu Thr Lys 
            20                  25                  30  


<210>  46
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; Hex-1

<400>  46

Asp Glu Glu Glu Ala Val Arg His Asn Asn Asn Val Leu Ala Lys Ala 
1               5                   10                  15      


Val Glu Asp Met Leu Lys Ala Val Glu Asp Asn Asn Arg His 
            20                  25                  30  


<210>  47
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-1

<400>  47

Asp Arg Lys Glu Glu Trp Asp Arg Asn Ala Lys His Ile Glu Glu Arg 
1               5                   10                  15      


Ala Arg Glu Trp Leu Lys Arg Met Glu Asp Arg Thr Arg Glu 
            20                  25                  30  


<210>  48
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-2

<400>  48

Asp Ala Met Lys Trp Ala Met Asp Ser Asn Thr Glu Val Ala Glu Met 
1               5                   10                  15      


Ala Trp Arg Ala Phe His Trp Ala Val Arg Leu Arg Glu Lys 
            20                  25                  30  


<210>  49
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; Hex-2

<400>  49

Asp Glu Glu Lys Trp Phe Arg Asp Ser His Arg Arg Ile Arg Glu Trp 
1               5                   10                  15      


Glu Glu Arg Met Arg Glu Leu Tyr Glu Arg Ala Glu Arg Arg 
            20                  25                  30  


<210>  50
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-3

<400>  50

Thr Glu Lys Glu Leu Ile Lys Trp Leu Ala Lys Ala Met Lys Asp Ala 
1               5                   10                  15      


Ile Arg Ile Ile Glu Glu Asn Asn Arg Trp Leu Arg Glu Ser 
            20                  25                  30  


<210>  51
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-3

<400>  51

Asp Glu Glu Ala Glu Arg Glu Trp Arg Asp Leu Lys Arg Trp Val Glu 
1               5                   10                  15      


Glu Leu Lys Arg Arg Ser Glu Glu Glu Trp Arg Arg Ala Asn 
            20                  25                  30  


<210>  52
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-4

<400>  52

Ser Glu Glu Glu Val Ala Arg His Ile Val Lys Ile Ala Glu Trp Phe 
1               5                   10                  15      


Arg Thr Leu Val Lys Ala Phe Glu Ser Asn Val Arg Ser Gln 
            20                  25                  30  


<210>  53
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; hex-4

<400>  53

Ser Lys Lys Ala Glu Asp Asp Ala Arg Lys Ala Asp Asp Glu Ala Arg 
1               5                   10                  15      


Lys Ala Trp Glu Arg Leu Lys Glu Leu Leu Asp Arg Gln Asn 
            20                  25                  30  


<210>  54
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E2 domain D

<400>  54

Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr Gln His Lys 
1               5                   10              


<210>  55
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E2 domain E

<400>  55

Asn Ile Gln Leu Ile Asn Thr Asn Gly Ser Trp His Ile Asn Ser 
1               5                   10                  15  


<210>  56
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E2 domain E

<400>  56

Glu Thr His Val Thr Gly Gly Ser Ala Gly Arg Thr Thr Ala Gly Leu 
1               5                   10                  15      


Val Gly Leu Leu Thr Pro Gly Ala Lys Gln Asn Ile Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Ile Asn 
        35                  40  


<210>  57
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E1 N-terminus

<400>  57

Tyr Gln Val Arg Asn Ser Ser Gly Leu Tyr His Val Thr Asn Asp 
1               5                   10                  15  


<210>  58
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; E1 ectodomain

<400>  58

Thr Gly His Arg Met Ala Trp Asp Met Met Met Asn 
1               5                   10          


<210>  59
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct; His tag

<400>  59

His His His His His His 
1               5       


