                         SEQUENCE LISTING

<110>  NEW YORK UNIVERSITY
 
<120>  NPC1 MONOBODIES AND MONOBODY CONJUGATES THEREOF

<130>  147462.002251

<140>  PCT/US2021/058783
<141>  2021-11-10

<150>  63/112,031
<151>  2020-11-10

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  94
<212>  PRT
<213>  Homo sapiens

<400>  1

Val Ser Asp Val Pro Arg Asp Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Arg Tyr Tyr 
            20                  25                  30          


Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln Glu Phe 
        35                  40                  45              


Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys Pro 
    50                  55                  60                  


Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Val Thr Gly Arg Gly Asp 
65                  70                  75                  80  


Ser Pro Ala Ser Ser Lys Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  


<210>  2
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  2

Lys Met Tyr Ser Tyr Pro Tyr Trp Tyr Tyr Ser 
1               5                   10      


<210>  3
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  3

Trp Gly Gly Trp Lys Trp Tyr Ser 
1               5               


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  4

Tyr Ser Tyr Tyr Lys Gly Trp Tyr Trp Ser 
1               5                   10  


<210>  5
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  5

Tyr Ser Pro Tyr Tyr Pro Ala Pro Tyr Arg Ser Ser 
1               5                   10          


<210>  6
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  6

Tyr Ser Ser Ser Met His Phe Ser Ser Ser 
1               5                   10  


<210>  7
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  7

Tyr Ser Pro Tyr Tyr Pro Ala Pro Tyr Arg Ser Ser 
1               5                   10          


<210>  8
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  8

Tyr Ser Pro Tyr Tyr Pro Ala Pro Tyr Arg Ser Ser 
1               5                   10          


<210>  9
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  9

Lys Ser Tyr Met Tyr Gly Pro Pro Ser Tyr Lys Ser Ser 
1               5                   10              


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  10

Tyr Tyr Gly Gln Met Arg Tyr Tyr Ser 
1               5                   


<210>  11
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  11

Tyr Tyr Ser Ser Tyr Arg Tyr Trp Ser 
1               5                   


<210>  12
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  12

Ser Ser Ser Ser Ser Gly Ser Ser Ser Ser Ser Lys 
1               5                   10          


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FG Loop sequence

<400>  13

Tyr Asp Gly Phe Tyr Tyr Thr Asn Asn Asp Ser 
1               5                   10      


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DE Loop sequence

<400>  14

Pro Ser Ser Ser Ser 
1               5   


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  15

Ala Tyr Val Ser Tyr Trp Lys Val Arg 
1               5                   


<210>  16
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  16

Ala Arg Gly Val Pro Ile Trp Trp Gln Val Tyr 
1               5                   10      


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  17

Ala Gln Pro Met Tyr Arg Ser Val Ser 
1               5                   


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  18

Ala Pro Ala Val Thr Val Ser 
1               5           


<210>  19
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  19

Ala Ser Ser Ser Ser Val Ser 
1               5           


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  20

Ala Pro Ala Val Thr Val Val 
1               5           


<210>  21
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  BC Loop sequence

<400>  21

Ala Pro Ala Val Thr Val Asp 
1               5           


<210>  22
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DE Loop sequence

<400>  22

Pro Gly Ser Ser Ser 
1               5   


<210>  23
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  23

Gly Gly Pro Phe Trp His Tyr 
1               5           


<210>  24
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  24

Gly Gly Ser Tyr Trp His Tyr 
1               5           


<210>  25
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  25

Gly Ser Pro Tyr Trp His Tyr 
1               5           


<210>  26
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  26

Gly Gly His Tyr Trp His Trp 
1               5           


<210>  27
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  27

Gly Ala Pro Val Trp His Val 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  28

Gly Ala Ser Pro Tyr Tyr Tyr 
1               5           


<210>  29
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD Loop sequence

<400>  29

Gly Gly Asn Ser Pro Val 
1               5       


<210>  30
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DE Loop sequence

<400>  30

Pro Gly Ser Ser Tyr 
1               5   


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DE Loop sequence

<400>  31

Pro Gly Ser Lys Ser 
1               5   


<210>  32
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N8)

<400>  32

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Tyr Val Ser Tyr Trp Lys Val Arg 
            20                  25                  30          


Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln 
        35                  40                  45              


Glu Phe Thr Val Pro Ser Ser Ser Ser Thr Ala Thr Ile Ser Gly Leu 
    50                  55                  60                  


Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Lys Met Tyr Ser 
65                  70                  75                  80  


Tyr Pro Tyr Trp Tyr Tyr Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95  


<210>  33
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N16)

<400>  33

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Arg Gly Val Pro Ile Trp Trp Gln 
            20                  25                  30          


Val Tyr Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro 
        35                  40                  45              


Val Gln Glu Phe Thr Val Pro Gly Ser Ser Ser Thr Ala Thr Ile Ser 
    50                  55                  60                  


Gly Leu Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Trp Gly 
65                  70                  75                  80  


Gly Trp Lys Trp Tyr Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  


<210>  34
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N18)

<400>  34

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Gln Pro Met Tyr Arg Ser Val Ser 
            20                  25                  30          


Tyr Tyr Arg Ile Thr Tyr Gly Glu Thr Gly Gly Asn Ser Pro Val Gln 
        35                  40                  45              


Glu Phe Thr Val Pro Gly Ser Ser Tyr Thr Ala Thr Ile Ser Gly Leu 
    50                  55                  60                  


Lys Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Ser Tyr Tyr 
65                  70                  75                  80  


Lys Gly Trp Tyr Trp Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  


<210>  35
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N22)

<400>  35

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Ser Tyr Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Gly Pro Phe Trp His Tyr Gln Thr 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Ser Pro Tyr Tyr 
65                  70                  75                  80  


Pro Ala Pro Tyr Arg Ser Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95  


<210>  36
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N23)

<400>  36

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Ser Ser Ser Ser Val Ser Tyr Tyr 
            20                  25                  30          


Arg Ile Thr Tyr Gly Glu Thr Gly Gly Pro Phe Trp His Tyr Gln Thr 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Thr Tyr Ser Ser Ser Met 
65                  70                  75                  80  


His Phe Ser Ser Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90              


<210>  37
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N24)

<400>  37

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Ser Tyr Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Gly Ser Tyr Trp His Tyr Gln Thr 
        35                  40                  45              


Phe Lys Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Ser Pro Tyr Tyr 
65                  70                  75                  80  


Pro Ala Pro Tyr Arg Ser Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95  


<210>  38
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N26)

<400>  38

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Ser Tyr Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Ser Pro Tyr Trp His Tyr Gln Thr 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Ser Pro Tyr Tyr 
65                  70                  75                  80  


Pro Ala Pro Tyr Arg Ser Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95  


<210>  39
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N31)

<400>  39

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Ser Tyr Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Gly His Tyr Trp His Trp Gln Thr 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Lys Ser Tyr Met Tyr 
65                  70                  75                  80  


Gly Pro Pro Ser Tyr Lys Ser Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95      


<210>  40
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N34)

<400>  40

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Ser Tyr Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Gly His Tyr Trp His Trp Gln Thr 
        35                  40                  45              


Phe Lys Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Tyr Gly Gln Met 
65                  70                  75                  80  


Arg Tyr Tyr Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  41
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N35)

<400>  41

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Val Tyr Tyr 
            20                  25                  30          


Asp Ile Thr Tyr Gly Glu Thr Gly Gly Ser Tyr Trp His Tyr Gln Thr 
        35                  40                  45              


Phe Lys Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Tyr Ser Ser Tyr 
65                  70                  75                  80  


Arg Tyr Trp Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90          


<210>  42
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1N-N38)

<400>  42

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Asp His Tyr 
            20                  25                  30          


Phe Ile Thr Tyr Gly Glu Thr Gly Ala Pro Val Trp His Val Gln Lys 
        35                  40                  45              


Phe Thr Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Ser Ser Ser Ser Ser 
65                  70                  75                  80  


Gly Ser Ser Ser Ser Ser Lys Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  95  


<210>  43
<211>  94
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mb(NPC1C-C45)

<400>  43

Val Ser Ser Val Pro Thr Lys Leu Glu Val Val Ala Ala Thr Pro Thr 
1               5                   10                  15      


Ser Leu Leu Ile Ser Trp Asp Ala Pro Ala Val Thr Val Val His Tyr 
            20                  25                  30          


Val Ile Thr Tyr Gly Glu Thr Gly Ala Ser Pro Tyr Tyr Tyr Gln Lys 
        35                  40                  45              


Phe Ala Val Pro Gly Ser Lys Ser Thr Ala Thr Ile Ser Gly Leu Lys 
    50                  55                  60                  


Pro Gly Val Asp Tyr Thr Ile Thr Val Tyr Ala Tyr Asp Gly Phe Tyr 
65                  70                  75                  80  


Tyr Thr Asn Asn Asp Ser Pro Ile Ser Ile Asn Tyr Arg Thr 
                85                  90                  


