                         SEQUENCE LISTING

<110>  Insitugen Limited
 
<120>  NOVEL STEROID HORMONE LIGAND ASSAYS

<130>  IGN1008/PC

<150>  US 63/112,974
<151>  2020-11-12

<160>  37    

<170>  PatentIn version 3.5

<210>  1
<211>  124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-crRecognition Sequence

<400>  1
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgga gaacagcctg       60

ttctccatct agaaatatgg attacttggt agaacagcaa tctactcgac ctgcaggcat      120

gcaa                                                                   124


<210>  2
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-crRecognition Sequence

<400>  2
cgcgtcaaat taatacgact cactataggg aactctggag gaagagcagg tcagcatgac       60

ctgttgcctc tagaaatatg gattacttgg tagaacagca atctactcga cctgcaggca      120

tgcaa                                                                  125


<210>  3
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-crRecognition Sequence

<400>  3
cgcgtcaaat taatacgact cactataggg aactctggag gaagagggta caaactgttc       60

ttctagaaat atggattact tggtagaaca gcaatctact cgacctgcag gcatgcaa        118


<210>  4
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-crRecognition Sequence

<400>  4
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgta caggatgttc       60

ttctagaaat atggattact tggtagaaca gcaatctact cgacctgcag gcatgcaa        118


<210>  5
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-crRecognition Sequence

<400>  5
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgta caggatgttc       60

ttctagaaat atggattact tggtagaaca gcaatctact cgacctgcag gcatgcaa        118


<210>  6
<211>  195
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-non/coding sequence

<400>  6
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgga gaacagcctg       60

ttctccaatg gggagaagcc tacgagcctg agcctccagt cttgccatgt gtatgtgggt      120

acgaaggaga ggagaggaag aggagagtac ccacatactc tgatgatcct tcgggatcat      180

tcatggcaat ctaga                                                       195


<210>  7
<211>  196
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-non/coding sequence

<400>  7
cgcgtcaaat taatacgact cactataggg aactctggag gaagagcagg tcagcatgac       60

ctgttgccat ggggagaagc ctacgagcct gagcctccag tcttgccatg tgtatgtggg      120

tacgaaggag aggagaggaa gaggagagta cccacatact ctgatgatcc ttcgggatca      180

ttcatggcaa tctaga                                                      196


<210>  8
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-non/coding sequence

<400>  8
cgcgtcaaat taatacgact cactataggg aactctggag gaagagggta caaactgttc       60

tatggggaga agcctacgag cctgagcctc cagtcttgcc atgtgtatgt gggtacgaag      120

gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga tcattcatgg      180

caatctaga                                                              189


<210>  9
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-non/coding sequence

<400>  9
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgta caggatgttc       60

tatggggaga agcctacgag cctgagcctc cagtcttgcc atgtgtatgt gggtacgaag      120

gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga tcattcatgg      180

caatctaga                                                              189


<210>  10
<211>  189
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-non/coding sequence

<400>  10
cgcgtcaaat taatacgact cactataggg aactctggag gaagagtgta caggatgttc       60

tatggggaga agcctacgag cctgagcctc cagtcttgcc atgtgtatgt gggtacgaag      120

gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga tcattcatgg      180

caatctaga                                                              189


<210>  11
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  11
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgt                      46


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  12
taatacgact cactatag                                                     18


<210>  13
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  13
taatacgact cactataggg aga                                               23


<210>  14
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  14
taatacgact cacaatcgcg gag                                               23


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3 RNA polymerase promoter/initiator sequence

<400>  15
aattaaccct cactaaag                                                     18


<210>  16
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6 RNA polymerase promoter/initiator sequence

<400>  16
atttaggtga cactatag                                                     18


<210>  17
<211>  64
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA sequence

<400>  17
uaccuaauga accaucuugu cguuagauca aaaucagggg aagcaaaaac cccaucagau       60

uuag                                                                    64


<210>  18
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 1 - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  18
agaacannnt gttct                                                        15


<210>  19
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 1 - Antisense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  19
tcttgtnnna caaga                                                        15


<210>  20
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 2 - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  20
ggtacannnt gttct                                                        15


<210>  21
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 2 - Antisense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  21
ccatgtnnna caaga                                                        15


<210>  22
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Estrogen Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  22
aggtcannnt gacct                                                        15


<210>  23
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Estrogen Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  23
tccagtnnna ctgga                                                        15


<210>  24
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Progesterone Response Element - Sense Strand

<400>  24
ggtacaaact gttct                                                        15


<210>  25
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Progesterone Response Element - Antisense Strand

<400>  25
ccatgtttga caaga                                                        15


<210>  26
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glucocorticoid/Mineralocorticoid Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<400>  26
agaacanaat gttct                                                        15


<210>  27
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glucocorticoid/Mineralocorticoid Response Element - Antisense 
       Strand


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<400>  27
tcttgtntta caaga                                                        15


<210>  28
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  15bp Spacer Sequence

<400>  28
actctggagg aagag                                                        15


<210>  29
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element

<400>  29
tggagaacag cctgttctcc a                                                 21


<210>  30
<211>  128
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mango II RNA Aptamer + F30 scaffold

<400>  30
atggggagaa gcctacgagc ctgagcctcc agtcttgcca tgtgtatgtg ggtacgaagg       60

agaggagagg aagaggagag tacccacata ctctgatgat ccttcgggat cattcatggc      120

aatctaga                                                               128


<210>  31
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CRISPR Recognition Sequence

<400>  31
atggattact tggtagaaca gcaatc                                            26


<210>  32
<211>  26
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  crRNA domain complementary to CRISPR recognition sequence

<400>  32
uaccuaauga accaucuugu cguuag                                            26


<210>  33
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  33
aggccggaga attgtaatac gactcactat agggagacgc gtgt                        44


<210>  34
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  34
caaattaata cgactcacta taggga                                            26


<210>  35
<211>  2
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2bp Spacer Sequence

<400>  35
ac                                                                       2


<210>  36
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-crRecognition Sequence

<400>  36
cgcgcaaatt aatacgactc actataggga acagaacagc ctgttctatg gattacttgg       60

tagaacagca atctactcga cctgcaggca tgcaa                                  95


<210>  37
<211>  110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-crRecognition Sequence

<400>  37
cgcgcaaatt aatacgactc actataggga actggagaac agcctgttct ccatctagaa       60

atatggatta cttggtagaa cagcaatcta ctcgacctgc aggcatgcaa                 110


