                         SEQUENCE LISTING

<110>  Insitugen Limited
 
<120>  NOVEL STEROID HORMONE LIGAND ASSAYS

<130>  IGN1007PC

<150>  US 63/112,924
<151>  2020-11-12

<160>  100   

<170>  PatentIn version 3.5

<210>  1
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  1
taatacgact cactatag                                                     18


<210>  2
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3 RNA polymerase promoter/initiator sequence

<400>  2
aattaaccct cactaaag                                                     18


<210>  3
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6 RNA polymerase promoter/initiator sequence

<400>  3
atttaggtga cactatag                                                     18


<210>  4
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 1 - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  4
agaacannnt gttct                                                        15


<210>  5
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 1 - Antisense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  5
tcttgtnnna caaga                                                        15


<210>  6
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 2 - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  6
ggtacannnt gttct                                                        15


<210>  7
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element 2 - Antisense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  7
ccatgtnnna caaga                                                        15


<210>  8
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Estrogen Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  8
aggtcannnt gacct                                                        15


<210>  9
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Estrogen Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(9)
<223>  n is a, c, g, or t

<400>  9
tccagtnnna ctgga                                                        15


<210>  10
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Progesterone Response Element - Sense Strand

<400>  10
ggtacaaact gttct                                                        15


<210>  11
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Progesterone Response Element - Antisense Strand

<400>  11
ccatgtttga caaga                                                        15


<210>  12
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glucocorticoid/Mineralocorticoid Response Element - Sense Strand


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<400>  12
agaacanaat gttct                                                        15


<210>  13
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Glucocorticoid/Mineralocorticoid Response Element - Antisense 
       Strand


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  n is a, c, g, or t

<400>  13
tcttgtntta caaga                                                        15


<210>  14
<211>  174
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-F30MangoII

<400>  14
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

gagaacagcc tgttctccat tgccatgtgt atgtgggtac gaaggagagg agaggaagag      120

gagagtaccc acatactctg atgatccttc gggatcattc atggcaatct agga            174


<210>  15
<211>  170
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-F30MangoII

<400>  15
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaaca       60

ggtcagcatg acctgttgcc atgtgtatgt gggtacgaag gagaggagag gaagaggaga      120

gtacccacat actctgatga tccttcggga tcattcatgg caatctagga                 170


<210>  16
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-F30MangoII

<400>  16
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaagg       60

tacaaactgt tctttgccat gtgtatgtgg gtacgaagga gaggagagga agaggagagt      120

acccacatac tctgatgatc cttcgggatc attcatggca atctagga                   168


<210>  17
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-F30MangoII

<400>  17
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctttgccat gtgtatgtgg gtacgaagga gaggagagga agaggagagt      120

acccacatac tctgatgatc cttcgggatc attcatggca atctagga                   168


<210>  18
<211>  168
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-F30MangoII

<400>  18
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctttgccat gtgtatgtgg gtacgaagga gaggagagga agaggagagt      120

acccacatac tctgatgatc cttcgggatc attcatggca atctagga                   168


<210>  19
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-MangoII

<400>  19
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

gagaacagcc tgttctccat acgaaggaga ggagaggaag aggagagta                  109


<210>  20
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-MangoII

<400>  20
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaaca       60

ggtcagcatg acctgtacga aggagaggag aggaagagga gagta                      105


<210>  21
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-MangoII

<400>  21
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaagg       60

tacaaactgt tcttacgaag gagaggagag gaagaggaga gta                        103


<210>  22
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-MangoII

<400>  22
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tcttacgaag gagaggagag gaagaggaga gta                        103


<210>  23
<211>  103
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-MangoII

<400>  23
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tcttacgaag gagaggagag gaagaggaga gta                        103


<210>  24
<211>  148
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-iSpinach

<400>  24
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

gagaacagcc tgttctccaa ggagtacggt gagggtcggg tccagtaggt acgcctactg      120

ttgagtagag tgtgggctcc gtactccc                                         148


<210>  25
<211>  144
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-iSpinach

<400>  25
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaaca       60

ggtcagcatg acctgaggag tacggtgagg gtcgggtcca gtaggtacgc ctactgttga      120

gtagagtgtg ggctccgtac tccc                                             144


<210>  26
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-iSpinach

<400>  26
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaagg       60

tacaaactgt tctaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt      120

agagtgtggg ctccgtactc cc                                               142


<210>  27
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-iSpinach

<400>  27
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt      120

agagtgtggg ctccgtactc cc                                               142


<210>  28
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-iSpinach

<400>  28
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt      120

agagtgtggg ctccgtactc cc                                               142


<210>  29
<211>  218
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-F30iSpinach

<400>  29
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

gagaacagcc tgttctccat tgccatgtgt atgtgggagg agtacggtga gggtcgggtc      120

cagtaggtac gcctactgtt gagtagagtg tgggctccgt actcccccca catactctga      180

tgatccttcg ggatcattca tggcaatcta gatctaga                              218


<210>  30
<211>  214
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ERE-F30iSpinach

<400>  30
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaaca       60

ggtcagcatg acctgttgcc atgtgtatgt gggaggagta cggtgagggt cgggtccagt      120

aggtacgcct actgttgagt agagtgtggg ctccgtactc cccccacata ctctgatgat      180

ccttcgggat cattcatggc aatctagatc taga                                  214


<210>  31
<211>  212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-PRE-F30iSpinach

<400>  31
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaagg       60

tacaaactgt tctttgccat gtgtatgtgg gaggagtacg gtgagggtcg ggtccagtag      120

gtacgcctac tgttgagtag agtgtgggct ccgtactccc cccacatact ctgatgatcc      180

ttcgggatca ttcatggcaa tctagatcta ga                                    212


<210>  32
<211>  212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-MRE-F30iSpinach

<400>  32
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctttgccat gtgtatgtgg gaggagtacg gtgagggtcg ggtccagtag      120

gtacgcctac tgttgagtag agtgtgggct ccgtactccc cccacatact ctgatgatcc      180

ttcgggatca ttcatggcaa tctagatcta ga                                    212


<210>  33
<211>  212
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-GRE-F30iSpinach

<400>  33
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

tacaggatgt tctttgccat gtgtatgtgg gaggagtacg gtgagggtcg ggtccagtag      120

gtacgcctac tgttgagtag agtgtgggct ccgtactccc cccacatact ctgatgatcc      180

ttcgggatca ttcatggcaa tctagatcta ga                                    212


<210>  34
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ARE-MangoII

<400>  34
aattaaccct cactaaagac tctggaggaa tggagaacag cctgttctcc atacgaagga       60

gaggagagga agaggagagt a                                                 81


<210>  35
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ERE-MangoII

<400>  35
aattaaccct cactaaagac tctggaggaa caggtcagca tgacctgtac gaaggagagg       60

agaggaagag gagagta                                                      77


<210>  36
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-PRE-MangoII

<400>  36
aattaaccct cactaaagac tctggaggaa ggtacaaact gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  37
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-MRE-MangoII

<400>  37
aattaaccct cactaaagac tctggaggaa tgtacaggat gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  38
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-GRE-MangoII

<400>  38
aattaaccct cactaaagac tctggaggaa tgtacaggat gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  39
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ARE-F30MangoII

<400>  39
aattaaccct cactaaagac tctggaggaa tggagaacag cctgttctcc attgccatgt       60

gtatgtgggt acgaaggaga ggagaggaag aggagagtac ccacatactc tgatgatcct      120

tcgggatcat tcatggcaat ctagga                                           146


<210>  40
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ERE-F30MangoII

<400>  40
aattaaccct cactaaagac tctggaggaa caggtcagca tgacctgttg ccatgtgtat       60

gtgggtacga aggagaggag aggaagagga gagtacccac atactctgat gatccttcgg      120

gatcattcat ggcaatctag ga                                               142


<210>  41
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-PRE-F30MangoII

<400>  41
aattaaccct cactaaagac tctggaggaa ggtacaaact gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  42
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-MRE-F30MangoII

<400>  42
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  43
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-GRE-F30MangoII

<400>  43
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  44
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ARE-iSpinach

<400>  44
aattaaccct cactaaagac tctggaggaa tggagaacag cctgttctcc aaggagtacg       60

gtgagggtcg ggtccagtag gtacgcctac tgttgagtag agtgtgggct ccgtactccc      120


<210>  45
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ERE-iSpinach

<400>  45
aattaaccct cactaaagac tctggaggaa caggtcagca tgacctgagg agtacggtga       60

gggtcgggtc cagtaggtac gcctactgtt gagtagagtg tgggctccgt actccc          116


<210>  46
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-PRE-iSpinach

<400>  46
aattaaccct cactaaagac tctggaggaa ggtacaaact gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  47
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-MRE-iSpinach

<400>  47
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  48
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-GRE-iSpinach

<400>  48
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  49
<211>  190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ARE-F30iSpinach

<400>  49
aattaaccct cactaaagac tctggaggaa tggagaacag cctgttctcc attgccatgt       60

gtatgtggga ggagtacggt gagggtcggg tccagtaggt acgcctactg ttgagtagag      120

tgtgggctcc gtactccccc cacatactct gatgatcctt cgggatcatt catggcaatc      180

tagatctaga                                                             190


<210>  50
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-ERE- F30iSpinach

<400>  50
aattaaccct cactaaagac tctggaggaa caggtcagca tgacctgttg ccatgtgtat       60

gtgggaggag tacggtgagg gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg      120

ggctccgtac tccccccaca tactctgatg atccttcggg atcattcatg gcaatctaga      180

tctaga                                                                 186


<210>  51
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-PRE- F30iSpinach

<400>  51
aattaaccct cactaaagac tctggaggaa ggtacaaact gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  52
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-MRE- F30iSpinach

<400>  52
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  53
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3i-GRE- F30iSpinach

<400>  53
aattaaccct cactaaagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  54
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ARE-MangoII

<400>  54
atttaggtga cactatagac tctggaggaa tggagaacag cctgttctcc atacgaagga       60

gaggagagga agaggagagt a                                                 81


<210>  55
<211>  77
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ERE-MangoII

<400>  55
atttaggtga cactatagac tctggaggaa caggtcagca tgacctgtac gaaggagagg       60

agaggaagag gagagta                                                      77


<210>  56
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-PRE-MangoII

<400>  56
atttaggtga cactatagac tctggaggaa ggtacaaact gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  57
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-MRE-MangoII

<400>  57
atttaggtga cactatagac tctggaggaa tgtacaggat gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  58
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-GRE-MangoII

<400>  58
atttaggtga cactatagac tctggaggaa tgtacaggat gttcttacga aggagaggag       60

aggaagagga gagta                                                        75


<210>  59
<211>  146
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ARE-F30MangoII

<400>  59
atttaggtga cactatagac tctggaggaa tggagaacag cctgttctcc attgccatgt       60

gtatgtgggt acgaaggaga ggagaggaag aggagagtac ccacatactc tgatgatcct      120

tcgggatcat tcatggcaat ctagga                                           146


<210>  60
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ERE-F30MangoII

<400>  60
atttaggtga cactatagac tctggaggaa caggtcagca tgacctgttg ccatgtgtat       60

gtgggtacga aggagaggag aggaagagga gagtacccac atactctgat gatccttcgg      120

gatcattcat ggcaatctag ga                                               142


<210>  61
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-PRE-F30MangoII

<400>  61
atttaggtga cactatagac tctggaggaa ggtacaaact gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  62
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-MRE-F30MangoII

<400>  62
atttaggtga cactatagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  63
<211>  140
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-GRE-F30MangoII

<400>  63
atttaggtga cactatagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggtacgaag gagaggagag gaagaggaga gtacccacat actctgatga tccttcggga      120

tcattcatgg caatctagga                                                  140


<210>  64
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ARE-iSpinach

<400>  64
atttaggtga cactatagac tctggaggaa tggagaacag cctgttctcc aaggagtacg       60

gtgagggtcg ggtccagtag gtacgcctac tgttgagtag agtgtgggct ccgtactccc      120


<210>  65
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ERE-iSpinach

<400>  65
atttaggtga cactatagac tctggaggaa caggtcagca tgacctgagg agtacggtga       60

gggtcgggtc cagtaggtac gcctactgtt gagtagagtg tgggctccgt actccc          116


<210>  66
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-PRE-iSpinach

<400>  66
atttaggtga cactatagac tctggaggaa ggtacaaact gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  67
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-MRE-iSpinach

<400>  67
atttaggtga cactatagac tctggaggaa tgtacaggat gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  68
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-GRE-iSpinach

<400>  68
atttaggtga cactatagac tctggaggaa tgtacaggat gttctaggag tacggtgagg       60

gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg ggctccgtac tccc            114


<210>  69
<211>  190
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ARE-F30iSpinach

<400>  69
atttaggtga cactatagac tctggaggaa tggagaacag cctgttctcc attgccatgt       60

gtatgtggga ggagtacggt gagggtcggg tccagtaggt acgcctactg ttgagtagag      120

tgtgggctcc gtactccccc cacatactct gatgatcctt cgggatcatt catggcaatc      180

tagatctaga                                                             190


<210>  70
<211>  186
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-ERE- F30iSpinach

<400>  70
atttaggtga cactatagac tctggaggaa caggtcagca tgacctgttg ccatgtgtat       60

gtgggaggag tacggtgagg gtcgggtcca gtaggtacgc ctactgttga gtagagtgtg      120

ggctccgtac tccccccaca tactctgatg atccttcggg atcattcatg gcaatctaga      180

tctaga                                                                 186


<210>  71
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-PRE- F30iSpinach

<400>  71
atttaggtga cactatagac tctggaggaa ggtacaaact gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  72
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-MRE-F30iSpinach

<400>  72
atttaggtga cactatagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  73
<211>  184
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SP6i-GRE-F30iSpinach

<400>  73
atttaggtga cactatagac tctggaggaa tgtacaggat gttctttgcc atgtgtatgt       60

gggaggagta cggtgagggt cgggtccagt aggtacgcct actgttgagt agagtgtggg      120

ctccgtactc cccccacata ctctgatgat ccttcgggat cattcatggc aatctagatc      180

taga                                                                   184


<210>  74
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  12 bp Spacer Sequence

<400>  74
actctggagg aa                                                           12


<210>  75
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3xTandem Androgen Response Element

<400>  75
aagcttagaa cagtttgtaa cgagctcgtt acaaactgtt ctagctcgtt acaaactgtt       60

ctaagct                                                                 67


<210>  76
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element

<400>  76
tggagaacag cctgttctcc a                                                 21


<210>  77
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MangoII Aptamer + F30 Scaffold

<400>  77
ttgccatgtg tatgtgggta cgaaggagag gagaggaaga ggagagtacc cacatactct       60

gatgatcctt cgggatcatt catggcaatc tagga                                  95


<210>  78
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MangoII Aptamer

<400>  78
tacgaaggag aggagaggaa gaggagagta                                        30


<210>  79
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Estrogen Response Element

<400>  79
caggtcagca tgacctg                                                      17


<210>  80
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hormone Response Element hexameric palindrome sequence

<400>  80
tgttct                                                                   6


<210>  81
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hormone Response Element hexameric palindrome sequence

<400>  81
tgacct                                                                   6


<210>  82
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  82
taatacgact cactatag                                                     18


<210>  83
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  83
taatacgact cactataggg aga                                               23


<210>  84
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  84
taatacgact cacaatcgcg gag                                               23


<210>  85
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 RNA polymerase promoter/initiator sequence

<400>  85
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgt                      46


<210>  86
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  15 bp Spacer Sequence

<400>  86
actctggagg aacat                                                        15


<210>  87
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  9 bp Spacer Sequence

<400>  87
actctggag                                                                9


<210>  88
<211>  2
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2 bp Spacer Sequence

<400>  88
ac                                                                       2


<210>  89
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  18 bp Spacer Sequence

<400>  89
actctggagg aacatatt                                                     18


<210>  90
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  21 bp Spacer Sequence

<400>  90
actctggagg aacatattgt a                                                 21


<210>  91
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  24 bp Spacer Sequence

<400>  91
actctggagg aacatattgt atcg                                              24


<210>  92
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  27 bp Spacer Sequence

<400>  92
actctggagg aacatattgt atcgatt                                           27


<210>  93
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Response Element

<400>  93
agaacagcct gttct                                                        15


<210>  94
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Androgen Response Element

<400>  94
aagaacagcc tgttcta                                                      17


<210>  95
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Androgen Response Element

<400>  95
tagaacagcc tgttctt                                                      17


<210>  96
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Androgen Response Element

<400>  96
agaacatcct gttct                                                        15


<210>  97
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified Androgen Response Element

<400>  97
agaacatcat gttct                                                        15


<210>  98
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Receptor Binding Site

<400>  98
agaaca                                                                   6


<210>  99
<211>  6
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Androgen Receptor Binding Site

<400>  99
tgttct                                                                   6


<210>  100
<211>  387
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7i-ARE-F30MangoII-T7i-ERE-iSpinach

<400>  100
ggaggccgga gaattgtaat acgactcact atagggagac gcgtgtactc tggaggaatg       60

gagaacagcc tgttctccat tgccatgtgt atgtgggtac gaaggagagg agaggaagag      120

gagagtaccc acatactctg atgatccttc gggatcattc atggcaatct aggaaacccc      180

gcggggcctt tcgggggtct cgcggggttt tttgctcgga ggccggagaa ttgtaatacg      240

actcactata gggagacgcg tgtactctgg aggaacaggt cagcatgacc tgttgccatg      300

tgtatgtggg tacgaaggag aggagaggaa gaggagagta cccacatact ctgatgatcc      360

ttcgggatca ttcatggcaa tctagga                                          387


