               SEQUENCE LISTING

<110> Technische Universität München

<120> Bioorthogonal reporter gene system

<130> AD1553 PCT

<140> PCT/EP2021/081775
<141> 2021-11-16

<150> EP 20 207 716.0
<151> 2020-11-16

<160> 30

<170> BiSSAP 1.3.6

<210> 1
<211> 60
<212> DNA
<213> Homo sapiens


<220> 
<223> Lcn2 signal peptide

<400> 1
atgcctctcg gactgctgtg gcttggactg gctctgctgg gagcactgca tgctcaggcc     60


<210> 2
<211> 20
<212> PRT
<213> Homo sapiens


<220> 
<223> Lcn2 signal peptide

<400> 2
Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      
His Ala Gln Ala 
            20  

<210> 3
<211> 531
<212> DNA
<213> Artificial Sequence


<220> 
<223> DTPA-binding Anticalin CL31d(A145T)

<400> 3
caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag      60

aacttccagg acaaccaatt ccatgggaag tggtatcaag tgggcagagc cggaaatgcc     120

gctcctcctg aagatcctga actgctgctg ctgacagccc agacctacga gctgaaagag     180

gacaagagct acgacgtgac cgccgtgcgg ttccggaaga agatgtgcga gtacctgacc     240

atgaccttcg tgcctggatc tcagcctggc gagttcaccc tgggcaacat caagaactac     300

cccggcctga ccagctactt cgtcagagtg gtgtccacca actacaacca gcacgccatg     360

gtgttcttca agaaggtcca gcagaaccgc gagtacttca gcatctctct gctcggccgg     420

accaaagagc tgaccagcga actgaaagag aacttcatcc ggttctccaa atctctgggc     480

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga t              531


<210> 4
<211> 177
<212> PRT
<213> Artificial Sequence


<220> 
<223> DTPA-binding Anticalin CL31d(A145T)

<400> 4
Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Lys Trp Tyr 
            20                  25                  30          
Gln Val Gly Arg Ala Gly Asn Ala Ala Pro Pro Glu Asp Pro Glu Leu 
        35                  40                  45              
Leu Leu Leu Thr Ala Gln Thr Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asp Val Thr Ala Val Arg Phe Arg Lys Lys Met Cys Glu Tyr Leu Thr 
65                  70                  75                  80  
Met Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe Thr Leu Gly Asn 
                85                  90                  95      
Ile Lys Asn Tyr Pro Gly Leu Thr Ser Tyr Phe Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Lys Val Gln Gln 
        115                 120                 125             
Asn Arg Glu Tyr Phe Ser Ile Ser Leu Leu Gly Arg Thr Lys Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp 
    

<210> 5
<211> 531
<212> DNA
<213> Artificial Sequence


<220> 
<223> Colchicine-binding Anticalin D6.4(Q77E)

<400> 5
caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag      60

aacttccagg acaaccaatt ccatggggag tggtatgtgg taggtgtcgc cggcaatggt     120

ttcctcagag aagataaaga tccgattaaa atggcggcca ccatctacga attaaaagaa     180

gacaagtctt ataacgtgac ctttatgaag tttccaatga agaaatgcga gtacatgacc     240

gacacgctgg ttcctggaag tcagcccggc gagtttatcc ttggcaatat caagagtgaa     300

cctggataca catcttggct cgtgcgcgtc gtgtcaacaa actataatca gcacgctatg     360

gtgttcttta aggctgttca acagaatcgg gaggactttt tcattactct ttacggaagg     420

acaatagagc tgacctccga gctgaaggag aactttattc gattctccaa atctctgggc     480

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga t              531


<210> 6
<211> 177
<212> PRT
<213> Artificial Sequence


<220> 
<223> Colchicine-binding Anticalin D6.4(Q77E)

<400> 6
Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro Leu Ser Lys Val 
1               5                   10                  15      
Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His Gly Glu Trp Tyr 
            20                  25                  30          
Val Val Gly Val Ala Gly Asn Gly Phe Leu Arg Glu Asp Lys Asp Pro 
        35                  40                  45              
Ile Lys Met Ala Ala Thr Ile Tyr Glu Leu Lys Glu Asp Lys Ser Tyr 
    50                  55                  60                  
Asn Val Thr Phe Met Lys Phe Pro Met Lys Lys Cys Glu Tyr Met Thr 
65                  70                  75                  80  
Asp Thr Leu Val Pro Gly Ser Gln Pro Gly Glu Phe Ile Leu Gly Asn 
                85                  90                  95      
Ile Lys Ser Glu Pro Gly Tyr Thr Ser Trp Leu Val Arg Val Val Ser 
            100                 105                 110         
Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys Ala Val Gln Gln 
        115                 120                 125             
Asn Arg Glu Asp Phe Phe Ile Thr Leu Tyr Gly Arg Thr Ile Glu Leu 
    130                 135                 140                 
Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser Lys Ser Leu Gly 
145                 150                 155                 160 
Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile Asp Gln Cys Ile 
                165                 170                 175     
Asp 
    

<210> 7
<211> 63
<212> DNA
<213> Artificial Sequence


<220> 
<223> Peptide-linker containing V5-tag

<400> 7
gccagcggca agcccattcc taatcctctg ctgggcctcg attctactgg cgcgcctgct     60

tct                                                                   63


<210> 8
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> Peptide-linker containing V5-tag

<400> 8
Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  15      
Gly Ala Pro Ala Ser 
            20      

<210> 9
<211> 105
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD4 transmembrane domain

<400> 9
cctgttcaac ccatggctct gatcgtgctt ggcggagttg ctggactgct gctgtttatc     60

ggcctgggca tcttcttttg cgtgcggtgc agacataggc ggaga                    105


<210> 10
<211> 35
<212> PRT
<213> Homo sapiens


<220> 
<223> CD4 transmembrane domain

<400> 10
Pro Val Gln Pro Met Ala Leu Ile Val Leu Gly Gly Val Ala Gly Leu 
1               5                   10                  15      
Leu Leu Phe Ile Gly Leu Gly Ile Phe Phe Cys Val Arg Cys Arg His 
            20                  25                  30          
Arg Arg Arg 
        35  

<210> 11
<211> 111
<212> DNA
<213> Homo sapiens


<220> 
<223> CD28 transmembrane domain

<400> 11
cctagcaagc ctttttgggt gctcgtggtc gttggcggag tgctggcctg ttatagcctg     60

ctggtcaccg tggccttcat catcttttgg gtccgaagca agcggagcag a             111


<210> 12
<211> 37
<212> PRT
<213> Homo sapiens


<220> 
<223> CD28 transmembrane domain

<400> 12
Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala 
1               5                   10                  15      
Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg 
            20                  25                  30          
Ser Lys Arg Ser Arg 
        35          

<210> 13
<211> 96
<212> DNA
<213> Homo sapiens


<220> 
<223> CD55 GPI-anchor coding sequence

<400> 13
ggcactactt ccggaaccac cagactgctg agcggtcaca cgtgcttcac cctcacaggg     60

ttgcttggca cactggtgac catgggactc ctgaca                               96


<210> 14
<211> 32
<212> PRT
<213> Homo sapiens


<220> 
<223> CD55 GPI-anchor coding sequence

<400> 14
Gly Thr Thr Ser Gly Thr Thr Arg Leu Leu Ser Gly His Thr Cys Phe 
1               5                   10                  15      
Thr Leu Thr Gly Leu Leu Gly Thr Leu Val Thr Met Gly Leu Leu Thr 
            20                  25                  30          



<210> 15
<211> 90
<212> DNA
<213> Homo sapiens


<220> 
<223> CD59 GPI-anchor coding sequence

<400> 15
cagctcgaga acggaggcac ttctctgtcc gaaaagaccg tgctgctgct ggtcacaccc     60

ttccttgcag ctgcctggag cttgcaccct                                      90


<210> 16
<211> 30
<212> PRT
<213> Artificial Sequence


<220> 
<223> CD59 GPI-anchor coding sequence

<400> 16
Gln Leu Glu Asn Gly Gly Thr Ser Leu Ser Glu Lys Thr Val Leu Leu 
1               5                   10                  15      
Leu Val Thr Pro Phe Leu Ala Ala Ala Trp Ser Leu His Pro 
            20                  25                  30  

<210> 17
<211> 723
<212> DNA
<213> Artificial Sequence


<220> 
<223> mRuby3 fluorescent protein

<400> 17
gcctctggta ccgtgtccaa gggcgaagaa ctgatcaaag aaaacatgcg gatgaaggtg      60

gtcatggaag gcagcgtgaa cggccaccag ttcaagtgta caggcgaagg cgagggcaga     120

ccttacgaag gcgtgcaagt catgcggatc aaagtgatcg aaggcggccc tctgcctttt     180

gccttcgata tcctggccac cagctttatg tacggcagcc ggaccttcat caagtacccc     240

gccgacattc ccgatttctt caagcagagc ttccccgagg gcttcacctg ggagagagtg     300

accagatacg aggatggcgg cgtcgtgacc gtgacacagg ataccagtct ggaagatggc     360

gagctggtgt acaacgtgaa agtgcggggc gtgaacttcc ccagcaatgg ccctgtgatg     420

cagaaaaaga ccaaaggctg ggagcccaac accgagatga tgtaccctgc tgatggcggc     480

ctgagaggct acacagatat cgccctgaaa gtggacggcg gaggccatct gcactgcaac     540

ttcgtgacca cctatcggtc caagaaaacc gtgggcaaca tcaagatgcc cggggtgcac     600

gccgtggacc acagactgga aagaatcgaa gagagcgaca acgagacata cgtggtgcag     660

agagaagtgg ccgtcgccaa gtacagcaat ctcggaggcg gaatggacga gctgtacaag     720

taa                                                                   723


<210> 18
<211> 240
<212> PRT
<213> Artificial Sequence


<220> 
<223> mRuby3 fluorescent protein

<400> 18
Ala Ser Gly Thr Val Ser Lys Gly Glu Glu Leu Ile Lys Glu Asn Met 
1               5                   10                  15      
Arg Met Lys Val Val Met Glu Gly Ser Val Asn Gly His Gln Phe Lys 
            20                  25                  30          
Cys Thr Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Val Gln Val Met 
        35                  40                  45              
Arg Ile Lys Val Ile Glu Gly Gly Pro Leu Pro Phe Ala Phe Asp Ile 
    50                  55                  60                  
Leu Ala Thr Ser Phe Met Tyr Gly Ser Arg Thr Phe Ile Lys Tyr Pro 
65                  70                  75                  80  
Ala Asp Ile Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly Phe Thr 
                85                  90                  95      
Trp Glu Arg Val Thr Arg Tyr Glu Asp Gly Gly Val Val Thr Val Thr 
            100                 105                 110         
Gln Asp Thr Ser Leu Glu Asp Gly Glu Leu Val Tyr Asn Val Lys Val 
        115                 120                 125             
Arg Gly Val Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys Lys Thr 
    130                 135                 140                 
Lys Gly Trp Glu Pro Asn Thr Glu Met Met Tyr Pro Ala Asp Gly Gly 
145                 150                 155                 160 
Leu Arg Gly Tyr Thr Asp Ile Ala Leu Lys Val Asp Gly Gly Gly His 
                165                 170                 175     
Leu His Cys Asn Phe Val Thr Thr Tyr Arg Ser Lys Lys Thr Val Gly 
            180                 185                 190         
Asn Ile Lys Met Pro Gly Val His Ala Val Asp His Arg Leu Glu Arg 
        195                 200                 205             
Ile Glu Glu Ser Asp Asn Glu Thr Tyr Val Val Gln Arg Glu Val Ala 
    210                 215                 220                 
Val Ala Lys Tyr Ser Asn Leu Gly Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 240 



<210> 19
<211> 945
<212> DNA
<213> Artificial Sequence


<220> 
<223> miRFP720 fluorescent protein

<400> 19
gcggagggga gcgtagccag gcagcctgat ttgctcactt gtgatgacga accgattcac      60

atccctggag caatacagcc tcacgggcta ctgctcgcgt tagcggctga catgactatt     120

gttgcgggca gcgataactt accagagcta acgggacttg caatcggggc tttgataggg     180

cgatctgctg cagacgtgtt cgattcggag acgcataacc gtttgaccat cgctttggct     240

gagccggggg cagctgtcgg agcccctatt actgtcggct ttactatgcg caaggacgct     300

ggattcatcg gctcttggca tcggcacgat cagctgattt ttcttgaact tgaaccaccc     360

cagcgagatg tggccgaacc ccaagcattc tttcgcagaa caaactcagc catacgtcgg     420

ctccaagcag cagagaccct cgagtcagct tgcgccgctg ccgctcagga agtccgaaaa     480

atcacaggtt ttgatagggt tatgatatat cggttcgcca gtgattttag tggtgaagtc     540

attgcagagg accgctgtgc cgaagtagag tctaaactgg gactgcacta ccctgcctcc     600

tttatccctg cccaggcccg cagactgtac accattaatc cagtccggat aattccagac     660

atcaattata ggcccgttcc agttacacca gaccttaatc ccgtgacagg cagacccatt     720

gacctctcct tcgccattct ccggagcgtg agccccgtgc atctggaatt catgcgcaac     780

atcggcatgc acggtaccat gtccatctcc atcctgaggg gtgagaggct gtggggcctg     840

atcgtgtgcc accataggac cccctattac gtggacctgg acggaagaca ggcctgcaag     900

agagtggccg agagactggc cacccaaatc ggcgtgatgg aggag                     945


<210> 20
<211> 315
<212> PRT
<213> Artificial Sequence


<220> 
<223> miRFP720 fluorescent protein

<400> 20
Ala Glu Gly Ser Val Ala Arg Gln Pro Asp Leu Leu Thr Cys Asp Asp 
1               5                   10                  15      
Glu Pro Ile His Ile Pro Gly Ala Ile Gln Pro His Gly Leu Leu Leu 
            20                  25                  30          
Ala Leu Ala Ala Asp Met Thr Ile Val Ala Gly Ser Asp Asn Leu Pro 
        35                  40                  45              
Glu Leu Thr Gly Leu Ala Ile Gly Ala Leu Ile Gly Arg Ser Ala Ala 
    50                  55                  60                  
Asp Val Phe Asp Ser Glu Thr His Asn Arg Leu Thr Ile Ala Leu Ala 
65                  70                  75                  80  
Glu Pro Gly Ala Ala Val Gly Ala Pro Ile Thr Val Gly Phe Thr Met 
                85                  90                  95      
Arg Lys Asp Ala Gly Phe Ile Gly Ser Trp His Arg His Asp Gln Leu 
            100                 105                 110         
Ile Phe Leu Glu Leu Glu Pro Pro Gln Arg Asp Val Ala Glu Pro Gln 
        115                 120                 125             
Ala Phe Phe Arg Arg Thr Asn Ser Ala Ile Arg Arg Leu Gln Ala Ala 
    130                 135                 140                 
Glu Thr Leu Glu Ser Ala Cys Ala Ala Ala Ala Gln Glu Val Arg Lys 
145                 150                 155                 160 
Ile Thr Gly Phe Asp Arg Val Met Ile Tyr Arg Phe Ala Ser Asp Phe 
                165                 170                 175     
Ser Gly Glu Val Ile Ala Glu Asp Arg Cys Ala Glu Val Glu Ser Lys 
            180                 185                 190         
Leu Gly Leu His Tyr Pro Ala Ser Phe Ile Pro Ala Gln Ala Arg Arg 
        195                 200                 205             
Leu Tyr Thr Ile Asn Pro Val Arg Ile Ile Pro Asp Ile Asn Tyr Arg 
    210                 215                 220                 
Pro Val Pro Val Thr Pro Asp Leu Asn Pro Val Thr Gly Arg Pro Ile 
225                 230                 235                 240 
Asp Leu Ser Phe Ala Ile Leu Arg Ser Val Ser Pro Val His Leu Glu 
                245                 250                 255     
Phe Met Arg Asn Ile Gly Met His Gly Thr Met Ser Ile Ser Ile Leu 
            260                 265                 270         
Arg Gly Glu Arg Leu Trp Gly Leu Ile Val Cys His His Arg Thr Pro 
        275                 280                 285             
Tyr Tyr Val Asp Leu Asp Gly Arg Gln Ala Cys Lys Arg Val Ala Glu 
    290                 295                 300                 
Arg Leu Ala Thr Gln Ile Gly Val Met Glu Glu 
305                 310                 315 

<210> 21
<211> 1482
<212> DNA
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD-mRuby3

<400> 21
atgcctctcg gactgctgtg gcttggactg gctctgctgg gagcactgca tgctcaggcc      60

caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag     120

aacttccagg acaaccaatt ccatgggaag tggtatcaag tgggcagagc cggaaatgcc     180

gctcctcctg aagatcctga actgctgctg ctgacagccc agacctacga gctgaaagag     240

gacaagagct acgacgtgac cgccgtgcgg ttccggaaga agatgtgcga gtacctgacc     300

atgaccttcg tgcctggatc tcagcctggc gagttcaccc tgggcaacat caagaactac     360

cccggcctga ccagctactt cgtcagagtg gtgtccacca actacaacca gcacgccatg     420

gtgttcttca agaaggtcca gcagaaccgc gagtacttca gcatctctct gctcggccgg     480

accaaagagc tgaccagcga actgaaagag aacttcatcc ggttctccaa atctctgggc     540

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga tgccagcggc     600

aagcccattc ctaatcctct gctgggcctc gattctactg gcgcgcctgc ttctcctgtt     660

caacccatgg ctctgatcgt gcttggcgga gttgctggac tgctgctgtt tatcggcctg     720

ggcatcttct tttgcgtgcg gtgcagacat aggcggagag cctctggtac cgtgtccaag     780

ggcgaagaac tgatcaaaga aaacatgcgg atgaaggtgg tcatggaagg cagcgtgaac     840

ggccaccagt tcaagtgtac aggcgaaggc gagggcagac cttacgaagg cgtgcaagtc     900

atgcggatca aagtgatcga aggcggccct ctgccttttg ccttcgatat cctggccacc     960

agctttatgt acggcagccg gaccttcatc aagtaccccg ccgacattcc cgatttcttc    1020

aagcagagct tccccgaggg cttcacctgg gagagagtga ccagatacga ggatggcggc    1080

gtcgtgaccg tgacacagga taccagtctg gaagatggcg agctggtgta caacgtgaaa    1140

gtgcggggcg tgaacttccc cagcaatggc cctgtgatgc agaaaaagac caaaggctgg    1200

gagcccaaca ccgagatgat gtaccctgct gatggcggcc tgagaggcta cacagatatc    1260

gccctgaaag tggacggcgg aggccatctg cactgcaact tcgtgaccac ctatcggtcc    1320

aagaaaaccg tgggcaacat caagatgccc ggggtgcacg ccgtggacca cagactggaa    1380

agaatcgaag agagcgacaa cgagacatac gtggtgcaga gagaagtggc cgtcgccaag    1440

tacagcaatc tcggaggcgg aatggacgag ctgtacaagt aa                       1482


<210> 22
<211> 493
<212> PRT
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD-mRuby3

<400> 22
Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      
His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          
Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 
        35                  40                  45              
Gly Lys Trp Tyr Gln Val Gly Arg Ala Gly Asn Ala Ala Pro Pro Glu 
    50                  55                  60                  
Asp Pro Glu Leu Leu Leu Leu Thr Ala Gln Thr Tyr Glu Leu Lys Glu 
65                  70                  75                  80  
Asp Lys Ser Tyr Asp Val Thr Ala Val Arg Phe Arg Lys Lys Met Cys 
                85                  90                  95      
Glu Tyr Leu Thr Met Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 
            100                 105                 110         
Thr Leu Gly Asn Ile Lys Asn Tyr Pro Gly Leu Thr Ser Tyr Phe Val 
        115                 120                 125             
Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 
Lys Val Gln Gln Asn Arg Glu Tyr Phe Ser Ile Ser Leu Leu Gly Arg 
145                 150                 155                 160 
Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     
Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         
Asp Gln Cys Ile Asp Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu 
        195                 200                 205             
Gly Leu Asp Ser Thr Gly Ala Pro Ala Ser Pro Val Gln Pro Met Ala 
    210                 215                 220                 
Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu 
225                 230                 235                 240 
Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Ala Ser Gly 
                245                 250                 255     
Thr Val Ser Lys Gly Glu Glu Leu Ile Lys Glu Asn Met Arg Met Lys 
            260                 265                 270         
Val Val Met Glu Gly Ser Val Asn Gly His Gln Phe Lys Cys Thr Gly 
        275                 280                 285             
Glu Gly Glu Gly Arg Pro Tyr Glu Gly Val Gln Val Met Arg Ile Lys 
    290                 295                 300                 
Val Ile Glu Gly Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr 
305                 310                 315                 320 
Ser Phe Met Tyr Gly Ser Arg Thr Phe Ile Lys Tyr Pro Ala Asp Ile 
                325                 330                 335     
Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg 
            340                 345                 350         
Val Thr Arg Tyr Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Thr 
        355                 360                 365             
Ser Leu Glu Asp Gly Glu Leu Val Tyr Asn Val Lys Val Arg Gly Val 
    370                 375                 380                 
Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys Lys Thr Lys Gly Trp 
385                 390                 395                 400 
Glu Pro Asn Thr Glu Met Met Tyr Pro Ala Asp Gly Gly Leu Arg Gly 
                405                 410                 415     
Tyr Thr Asp Ile Ala Leu Lys Val Asp Gly Gly Gly His Leu His Cys 
            420                 425                 430         
Asn Phe Val Thr Thr Tyr Arg Ser Lys Lys Thr Val Gly Asn Ile Lys 
        435                 440                 445             
Met Pro Gly Val His Ala Val Asp His Arg Leu Glu Arg Ile Glu Glu 
    450                 455                 460                 
Ser Asp Asn Glu Thr Tyr Val Val Gln Arg Glu Val Ala Val Ala Lys 
465                 470                 475                 480 
Tyr Ser Asn Leu Gly Gly Gly Met Asp Glu Leu Tyr Lys 
                485                 490             

<210> 23
<211> 774
<212> DNA
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD

<400> 23
atgcctctcg gactgctgtg gcttggactg gctctgctgg gagcactgca tgctcaggcc      60

caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag     120

aacttccagg acaaccaatt ccatgggaag tggtatcaag tgggcagagc cggaaatgcc     180

gctcctcctg aagatcctga actgctgctg ctgacagccc agacctacga gctgaaagag     240

gacaagagct acgacgtgac cgccgtgcgg ttccggaaga agatgtgcga gtacctgacc     300

atgaccttcg tgcctggatc tcagcctggc gagttcaccc tgggcaacat caagaactac     360

cccggcctga ccagctactt cgtcagagtg gtgtccacca actacaacca gcacgccatg     420

gtgttcttca agaaggtcca gcagaaccgc gagtacttca gcatctctct gctcggccgg     480

accaaagagc tgaccagcga actgaaagag aacttcatcc ggttctccaa atctctgggc     540

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga tgccagcggc     600

aagcccattc ctaatcctct gctgggcctc gattctactg gcgcgcctgc ttctcctgtt     660

caacccatgg ctctgatcgt gcttggcgga gttgctggac tgctgctgtt tatcggcctg     720

ggcatcttct tttgcgtgcg gtgcagacat aggcggagag cctctaccgg ttaa           774


<210> 24
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD

<400> 24
Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      
His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          
Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 
        35                  40                  45              
Gly Lys Trp Tyr Gln Val Gly Arg Ala Gly Asn Ala Ala Pro Pro Glu 
    50                  55                  60                  
Asp Pro Glu Leu Leu Leu Leu Thr Ala Gln Thr Tyr Glu Leu Lys Glu 
65                  70                  75                  80  
Asp Lys Ser Tyr Asp Val Thr Ala Val Arg Phe Arg Lys Lys Met Cys 
                85                  90                  95      
Glu Tyr Leu Thr Met Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 
            100                 105                 110         
Thr Leu Gly Asn Ile Lys Asn Tyr Pro Gly Leu Thr Ser Tyr Phe Val 
        115                 120                 125             
Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 
Lys Val Gln Gln Asn Arg Glu Tyr Phe Ser Ile Ser Leu Leu Gly Arg 
145                 150                 155                 160 
Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     
Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         
Asp Gln Cys Ile Asp Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu 
        195                 200                 205             
Gly Leu Asp Ser Thr Gly Ala Pro Ala Ser Pro Val Gln Pro Met Ala 
    210                 215                 220                 
Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu 
225                 230                 235                 240 
Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Ala Ser Thr 
                245                 250                 255     
Gly 
    

<210> 25
<211> 774
<212> DNA
<213> Artificial Sequence


<220> 
<223> Colchi-V5-TMD

<400> 25
atgcctctcg gactgctgtg gcttggactg gctctgctgg gagcactgca tgctcaggcc      60

caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag     120

aacttccagg acaaccaatt ccatggggag tggtatgtgg taggtgtcgc cggcaatggt     180

ttcctcagag aagataaaga tccgattaaa atggcggcca ccatctacga attaaaagaa     240

gacaagtctt ataacgtgac ctttatgaag tttccaatga agaaatgcga gtacatgacc     300

gacacgctgg ttcctggaag tcagcccggc gagtttatcc ttggcaatat caagagtgaa     360

cctggataca catcttggct cgtgcgcgtc gtgtcaacaa actataatca gcacgctatg     420

gtgttcttta aggctgttca acagaatcgg gaggactttt tcattactct ttacggaagg     480

acaatagagc tgacctccga gctgaaggag aactttattc gattctccaa atctctgggc     540

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga tgccagcggc     600

aagcccattc ctaatcctct gctgggcctc gattctactg gcgcgcctgc ttctcctgtt     660

caacccatgg ctctgatcgt gcttggcgga gttgctggac tgctgctgtt tatcggcctg     720

ggcatcttct tttgcgtgcg gtgcagacat aggcggagag cctctaccgg ttaa           774


<210> 26
<211> 257
<212> PRT
<213> Artificial Sequence


<220> 
<223> Colchi-V5-TMD

<400> 26
Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      
His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          
Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 
        35                  40                  45              
Gly Glu Trp Tyr Val Val Gly Val Ala Gly Asn Gly Phe Leu Arg Glu 
    50                  55                  60                  
Asp Lys Asp Pro Ile Lys Met Ala Ala Thr Ile Tyr Glu Leu Lys Glu 
65                  70                  75                  80  
Asp Lys Ser Tyr Asn Val Thr Phe Met Lys Phe Pro Met Lys Lys Cys 
                85                  90                  95      
Glu Tyr Met Thr Asp Thr Leu Val Pro Gly Ser Gln Pro Gly Glu Phe 
            100                 105                 110         
Ile Leu Gly Asn Ile Lys Ser Glu Pro Gly Tyr Thr Ser Trp Leu Val 
        115                 120                 125             
Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 
Ala Val Gln Gln Asn Arg Glu Asp Phe Phe Ile Thr Leu Tyr Gly Arg 
145                 150                 155                 160 
Thr Ile Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     
Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         
Asp Gln Cys Ile Asp Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu 
        195                 200                 205             
Gly Leu Asp Ser Thr Gly Ala Pro Ala Ser Pro Val Gln Pro Met Ala 
    210                 215                 220                 
Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu 
225                 230                 235                 240 
Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Ala Ser Thr 
                245                 250                 255     
Gly 
    

<210> 27
<211> 1719
<212> DNA
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD-miRFP720

<400> 27
atgcctctcg gactgctgtg gcttggactg gctctgctgg gagcactgca tgctcaggcc      60

caggatagca ccagcgatct gattccagct cctccactga gcaaggtgcc cctgcagcag     120

aacttccagg acaaccaatt ccatgggaag tggtatcaag tgggcagagc cggaaatgcc     180

gctcctcctg aagatcctga actgctgctg ctgacagccc agacctacga gctgaaagag     240

gacaagagct acgacgtgac cgccgtgcgg ttccggaaga agatgtgcga gtacctgacc     300

atgaccttcg tgcctggatc tcagcctggc gagttcaccc tgggcaacat caagaactac     360

cccggcctga ccagctactt cgtcagagtg gtgtccacca actacaacca gcacgccatg     420

gtgttcttca agaaggtcca gcagaaccgc gagtacttca gcatctctct gctcggccgg     480

accaaagagc tgaccagcga actgaaagag aacttcatcc ggttctccaa atctctgggc     540

ctgcccgaga atcacatcgt gttccccgtg cctatcgacc agtgcatcga tgccagcggc     600

aagcccattc ctaatcctct gctgggcctc gattctactg gcgcgcctgc ttctcctgtt     660

caacccatgg ctctgatcgt gcttggcgga gttgctggac tgctgctgtt tatcggcctg     720

ggcatcttct tttgcgtgcg gtgcagacat aggcggagag cctctaccgg tgcggagggg     780

agcgtagcca ggcagcctga tttgctcact tgtgatgacg aaccgattca catccctgga     840

gcaatacagc ctcacgggct actgctcgcg ttagcggctg acatgactat tgttgcgggc     900

agcgataact taccagagct aacgggactt gcaatcgggg ctttgatagg gcgatctgct     960

gcagacgtgt tcgattcgga gacgcataac cgtttgacca tcgctttggc tgagccgggg    1020

gcagctgtcg gagcccctat tactgtcggc tttactatgc gcaaggacgc tggattcatc    1080

ggctcttggc atcggcacga tcagctgatt tttcttgaac ttgaaccacc ccagcgagat    1140

gtggccgaac cccaagcatt ctttcgcaga acaaactcag ccatacgtcg gctccaagca    1200

gcagagaccc tcgagtcagc ttgcgccgct gccgctcagg aagtccgaaa aatcacaggt    1260

tttgataggg ttatgatata tcggttcgcc agtgatttta gtggtgaagt cattgcagag    1320

gaccgctgtg ccgaagtaga gtctaaactg ggactgcact accctgcctc ctttatccct    1380

gcccaggccc gcagactgta caccattaat ccagtccgga taattccaga catcaattat    1440

aggcccgttc cagttacacc agaccttaat cccgtgacag gcagacccat tgacctctcc    1500

ttcgccattc tccggagcgt gagccccgtg catctggaat tcatgcgcaa catcggcatg    1560

cacggtacca tgtccatctc catcctgagg ggtgagaggc tgtggggcct gatcgtgtgc    1620

caccatagga ccccctatta cgtggacctg gacggaagac aggcctgcaa gagagtggcc    1680

gagagactgg ccacccaaat cggcgtgatg gaggagtaa                           1719


<210> 28
<211> 572
<212> PRT
<213> Artificial Sequence


<220> 
<223> DTPA-V5-TMD-miRFP720

<400> 28
Met Pro Leu Gly Leu Leu Trp Leu Gly Leu Ala Leu Leu Gly Ala Leu 
1               5                   10                  15      
His Ala Gln Ala Gln Asp Ser Thr Ser Asp Leu Ile Pro Ala Pro Pro 
            20                  25                  30          
Leu Ser Lys Val Pro Leu Gln Gln Asn Phe Gln Asp Asn Gln Phe His 
        35                  40                  45              
Gly Lys Trp Tyr Gln Val Gly Arg Ala Gly Asn Ala Ala Pro Pro Glu 
    50                  55                  60                  
Asp Pro Glu Leu Leu Leu Leu Thr Ala Gln Thr Tyr Glu Leu Lys Glu 
65                  70                  75                  80  
Asp Lys Ser Tyr Asp Val Thr Ala Val Arg Phe Arg Lys Lys Met Cys 
                85                  90                  95      
Glu Tyr Leu Thr Met Thr Phe Val Pro Gly Ser Gln Pro Gly Glu Phe 
            100                 105                 110         
Thr Leu Gly Asn Ile Lys Asn Tyr Pro Gly Leu Thr Ser Tyr Phe Val 
        115                 120                 125             
Arg Val Val Ser Thr Asn Tyr Asn Gln His Ala Met Val Phe Phe Lys 
    130                 135                 140                 
Lys Val Gln Gln Asn Arg Glu Tyr Phe Ser Ile Ser Leu Leu Gly Arg 
145                 150                 155                 160 
Thr Lys Glu Leu Thr Ser Glu Leu Lys Glu Asn Phe Ile Arg Phe Ser 
                165                 170                 175     
Lys Ser Leu Gly Leu Pro Glu Asn His Ile Val Phe Pro Val Pro Ile 
            180                 185                 190         
Asp Gln Cys Ile Asp Ala Ser Gly Lys Pro Ile Pro Asn Pro Leu Leu 
        195                 200                 205             
Gly Leu Asp Ser Thr Gly Ala Pro Ala Ser Pro Val Gln Pro Met Ala 
    210                 215                 220                 
Leu Ile Val Leu Gly Gly Val Ala Gly Leu Leu Leu Phe Ile Gly Leu 
225                 230                 235                 240 
Gly Ile Phe Phe Cys Val Arg Cys Arg His Arg Arg Arg Ala Ser Thr 
                245                 250                 255     
Gly Ala Glu Gly Ser Val Ala Arg Gln Pro Asp Leu Leu Thr Cys Asp 
            260                 265                 270         
Asp Glu Pro Ile His Ile Pro Gly Ala Ile Gln Pro His Gly Leu Leu 
        275                 280                 285             
Leu Ala Leu Ala Ala Asp Met Thr Ile Val Ala Gly Ser Asp Asn Leu 
    290                 295                 300                 
Pro Glu Leu Thr Gly Leu Ala Ile Gly Ala Leu Ile Gly Arg Ser Ala 
305                 310                 315                 320 
Ala Asp Val Phe Asp Ser Glu Thr His Asn Arg Leu Thr Ile Ala Leu 
                325                 330                 335     
Ala Glu Pro Gly Ala Ala Val Gly Ala Pro Ile Thr Val Gly Phe Thr 
            340                 345                 350         
Met Arg Lys Asp Ala Gly Phe Ile Gly Ser Trp His Arg His Asp Gln 
        355                 360                 365             
Leu Ile Phe Leu Glu Leu Glu Pro Pro Gln Arg Asp Val Ala Glu Pro 
    370                 375                 380                 
Gln Ala Phe Phe Arg Arg Thr Asn Ser Ala Ile Arg Arg Leu Gln Ala 
385                 390                 395                 400 
Ala Glu Thr Leu Glu Ser Ala Cys Ala Ala Ala Ala Gln Glu Val Arg 
                405                 410                 415     
Lys Ile Thr Gly Phe Asp Arg Val Met Ile Tyr Arg Phe Ala Ser Asp 
            420                 425                 430         
Phe Ser Gly Glu Val Ile Ala Glu Asp Arg Cys Ala Glu Val Glu Ser 
        435                 440                 445             
Lys Leu Gly Leu His Tyr Pro Ala Ser Phe Ile Pro Ala Gln Ala Arg 
    450                 455                 460                 
Arg Leu Tyr Thr Ile Asn Pro Val Arg Ile Ile Pro Asp Ile Asn Tyr 
465                 470                 475                 480 
Arg Pro Val Pro Val Thr Pro Asp Leu Asn Pro Val Thr Gly Arg Pro 
                485                 490                 495     
Ile Asp Leu Ser Phe Ala Ile Leu Arg Ser Val Ser Pro Val His Leu 
            500                 505                 510         
Glu Phe Met Arg Asn Ile Gly Met His Gly Thr Met Ser Ile Ser Ile 
        515                 520                 525             
Leu Arg Gly Glu Arg Leu Trp Gly Leu Ile Val Cys His His Arg Thr 
    530                 535                 540                 
Pro Tyr Tyr Val Asp Leu Asp Gly Arg Gln Ala Cys Lys Arg Val Ala 
545                 550                 555                 560 
Glu Arg Leu Ala Thr Gln Ile Gly Val Met Glu Glu 
                565                 570         

<210> 29
<211> 6
<212> PRT
<213> Homo sapiens


<220> 
<223> Amyloid beta sequence recognized by an Anticalin

<400> 29
Val Phe Phe Ala Glu Asp 
1               5       

<210> 30
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> signal peptide

<400> 30
Met Gly Trp Ser Cys Ile Ile Leu Phe Leu Val Ala Thr Ala Thr Gly 
1               5                   10                  15      



