                         SEQUENCE LISTING

<110>  ɽ˴Ƽ޹˾
 
<120>  CasøϵͳԼӦ

<130>  111

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  1283
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ukcpf1

<400>  1

Met Ser Asn Met Gln Gln Tyr Asp Asn Phe Ile Asn His Tyr Ala Ile 
1               5                   10                  15      


Gln Lys Thr Leu Arg Phe Glu Leu Gln Pro Ile Gly Lys Thr Arg Glu 
            20                  25                  30          


His Ile Gln Lys Asn Gly Ile Ile Glu His Asp Glu Ala Leu Glu Gln 
        35                  40                  45              


Lys Tyr Gln Ile Val Lys Lys Ile Ile Asp Arg Phe His Arg Lys His 
    50                  55                  60                  


Ile Asp Glu Ala Leu Ser Leu Ala Asp Phe Ser Lys Asp Thr Ala Met 
65                  70                  75                  80  


Leu Lys Arg Phe Glu Glu Leu Tyr Trp Lys Lys Asn Lys Asn Glu Asn 
                85                  90                  95      


Glu Lys Asn Glu Phe Val Lys Ile Gln Ser Asp Leu Arg Lys Arg Val 
            100                 105                 110         


Val Ser Phe Leu Glu Gly Lys Val Glu Gly Asp Ala Arg Phe Ala Lys 
        115                 120                 125             


Val Gln Gln Arg Tyr Gly Ile Leu Phe Asp Ala Lys Ile Phe Lys Asp 
    130                 135                 140                 


Lys Glu Phe Ile Ser Thr Ala Cys Asp Asp Ile Glu Lys Asp Ala Ile 
145                 150                 155                 160 


Glu Ala Phe Lys Arg Phe Ala Thr Tyr Phe Thr Gly Phe His Glu Asn 
                165                 170                 175     


Arg Lys Asn Met Tyr Ser Ala Asp Glu Glu Ser Thr Ala Ile Ala Tyr 
            180                 185                 190         


Arg Val Ile Asn Glu Asn Leu Pro Arg Phe Leu Glu Asn Lys Ala Arg 
        195                 200                 205             


Phe Glu Lys Ile Gln His Thr Val Asp Ser Lys Thr Leu Asn Glu Ile 
    210                 215                 220                 


Ala Thr Glu Leu Lys Pro Val Leu Glu Lys Asn Lys Leu Glu Thr Ile 
225                 230                 235                 240 


Phe Thr Leu Asn Tyr Phe Gln Asn Thr Leu Ser Gln Ala Gly Ile Thr 
                245                 250                 255     


Tyr Tyr Asn Thr Ile Leu Gly Gly Lys Thr Lys Glu Asn Gly Glu Lys 
            260                 265                 270         


Val Gln Gly Leu Asn Glu Ile Ile Asn Leu Phe Asn Gln Lys Asn Lys 
        275                 280                 285             


Asp Thr Met Leu Pro Leu Leu Lys Pro Leu Tyr Lys Gln Ile Leu Ser 
    290                 295                 300                 


Glu Glu Tyr Ser Thr Ser Phe Thr Ile Ser Ala Phe Glu Lys Asp Asn 
305                 310                 315                 320 


Asp Val Leu Gln Ala Ile Gly Ser Phe Cys Asn Asp Cys Ile Phe Tyr 
                325                 330                 335     


Ala Lys Asn Asn Val Asn Gly Lys Ala Tyr Asn Leu Leu Gln Thr Val 
            340                 345                 350         


Gln Ala Phe Cys Asn Ser Ile Asp Thr Tyr Asn Asp Asn Arg Leu Asp 
        355                 360                 365             


Gly Leu His Ile Glu Arg Lys Asn Leu Ala Thr Leu Ser His Gln Val 
    370                 375                 380                 


Tyr Gly Glu Trp Asn Ile Leu Arg Asp Ala Leu Gln Ile His Tyr Glu 
385                 390                 395                 400 


Ala Tyr Glu Gln Lys Asp Asn Gly Asn Asn Asn Asn Tyr Leu Glu Ser 
                405                 410                 415     


Lys Thr Phe Ser Trp Lys Ala Leu Lys Asp Ala Leu Thr Thr Tyr Lys 
            420                 425                 430         


Ser Leu Val Glu Glu Ala Gln Asp Ile Asp Glu Asn Gly Phe Ile Ala 
        435                 440                 445             


Tyr Phe Lys Asp Met Lys Phe Lys Glu Glu Ile Asp Gly Lys Thr Thr 
    450                 455                 460                 


Ser Ile Asp Leu Ile Glu Asn Ile Gln Thr Arg Tyr Lys Ser Ile Glu 
465                 470                 475                 480 


Thr Ile Leu Gln Glu Asp Arg Asn Asn Lys Asn Asn Leu His Gln Glu 
                485                 490                 495     


Lys Glu Lys Val Ala Thr Ile Lys Gly Phe Leu Asp Ser Val Lys Tyr 
            500                 505                 510         


Leu Gln Trp Phe Leu Asn Leu Met Tyr Ile Ala Ser Pro Val Asp Asp 
        515                 520                 525             


Lys Asp Tyr Asp Phe Tyr Asn Glu Leu Glu Met Tyr His Asp Thr Leu 
    530                 535                 540                 


Leu Pro Leu Thr Thr Leu Tyr Asn Lys Val Arg Asn Tyr Met Thr Arg 
545                 550                 555                 560 


Lys Pro Tyr Ser Val Glu Lys Phe Lys Leu Thr Phe Glu Lys Ser Thr 
                565                 570                 575     


Leu Leu Asp Gly Trp Asp Lys Asn Lys Glu Arg Ala Asn Leu Gly Val 
            580                 585                 590         


Ile Leu Arg Lys Gly Asn Asn Tyr Tyr Leu Gly Ile Met Asn Lys Lys 
        595                 600                 605             


Tyr Asn Asp Ile Phe Asp Ser Ile Pro Gly Leu Thr Thr Thr Asp Tyr 
    610                 615                 620                 


Cys Glu Lys Met Asn Tyr Lys Leu Leu Pro Gly Pro Asn Lys Met Leu 
625                 630                 635                 640 


Pro Lys Val Phe Phe Ser Lys Lys Gly Val Gln Phe Tyr Lys Pro Ser 
                645                 650                 655     


Gln Glu Ile Ile Arg Leu Tyr Asn Asn Lys Glu Phe Lys Lys Gly Asp 
            660                 665                 670         


Thr Phe Asn Lys Asn Ser Leu His Lys Leu Ile Asn Phe Tyr Lys Glu 
        675                 680                 685             


Ser Ile Ala Lys Thr Glu Asp Trp Ser Val Phe Gln Phe Lys Phe Lys 
    690                 695                 700                 


Asn Thr Asn Asp Tyr Ala Asp Ile Ser Gln Phe Tyr Lys Asp Val Glu 
705                 710                 715                 720 


Arg Gln Gly Tyr Lys Ile Ser Phe Asp Lys Ile Asp Trp Glu Tyr Ile 
                725                 730                 735     


Leu Leu Leu Val Asp Glu Gly Lys Leu Phe Leu Phe Lys Ile Tyr Asn 
            740                 745                 750         


Lys Asp Phe Ser Pro Tyr Ser Lys Gly Lys Pro Asn Leu His Thr Ile 
        755                 760                 765             


Tyr Trp Lys Asn Ile Phe Ser His Asp Asn Leu Asn Asn Val Val Tyr 
    770                 775                 780                 


Lys Leu Asn Gly Glu Ala Glu Val Phe Tyr Arg Lys Lys Ser Ile Glu 
785                 790                 795                 800 


Tyr Pro Glu Glu Ile Leu Gln Lys Gly His His Val Asn Glu Leu Lys 
                805                 810                 815     


Asp Lys Phe Lys Tyr Pro Ile Ile Lys Asp Lys Arg Tyr Ala Glu Asp 
            820                 825                 830         


Lys Phe Leu Phe His Val Pro Ile Thr Met Asn Phe Leu Ser Lys Gly 
        835                 840                 845             


Glu Pro Asn Ile Asn Gln Arg Val Gln Gln Tyr Ile Ala Ser Thr Ser 
    850                 855                 860                 


Glu Asp Tyr His Ile Ile Gly Ile Asp Arg Gly Glu Arg Asn Leu Leu 
865                 870                 875                 880 


Tyr Leu Ser Leu Ile Asp Ala Thr Gly Lys Ile Ile Lys Gln Leu Ser 
                885                 890                 895     


Leu Asn Thr Ile Lys Asn Glu Asn Phe Asn Thr Thr Ile Asp Tyr His 
            900                 905                 910         


Ala Lys Leu Asp Glu Lys Glu Lys Lys Arg Glu Glu Ala Arg Lys Asn 
        915                 920                 925             


Trp Asp Val Ile Glu Asn Ile Lys Glu Leu Lys Glu Gly Tyr Leu Ser 
    930                 935                 940                 


Gln Val Val His Gln Ile Ala Lys Leu Met Val Glu Tyr Lys Ala Ile 
945                 950                 955                 960 


Leu Val Met Glu Asp Leu Asn Thr Gly Phe Lys Arg Gly Arg Phe Lys 
                965                 970                 975     


Val Glu Lys Gln Val Tyr Gln Lys Phe Glu Lys Met Met Ile Asp Lys 
            980                 985                 990         


Leu Asn Tyr Leu Val Leu Lys Asp  Arg Gln Ala Thr Gln  Pro Gly Gly 
        995                 1000                 1005             


Ser Leu  Lys Ala Tyr Gln Leu  Ala Ser Ser Leu Glu  Ser Phe Lys 
    1010                 1015                 1020             


Lys Leu  Gly Lys Gln Cys Gly  Met Ile Phe Tyr Val  Pro Ala Val 
    1025                 1030                 1035             


Tyr Thr  Ser Lys Ile Asp Pro  Thr Thr Gly Phe Tyr  Asn Phe Leu 
    1040                 1045                 1050             


Arg Val  Asp Val Ser Thr Leu  Asn Ser Ala His Ser  Phe Phe Asn 
    1055                 1060                 1065             


Arg Phe  Asn Ala Ile Val Tyr  Asn Asn Glu Gln Asp  Tyr Phe Glu 
    1070                 1075                 1080             


Phe His  Cys Thr Tyr Lys Asn  Phe Val Ser Glu Pro  Ser Leu Gln 
    1085                 1090                 1095             


Lys Asn  Val Lys Ser Ser Lys  Met His Glu Tyr Asn  Asn Leu Lys 
    1100                 1105                 1110             


Asp Thr  Thr Trp Val Leu Cys  Ser Thr His His Glu  Arg Tyr Lys 
    1115                 1120                 1125             


Lys Phe  Lys Asn Lys Ser Gly  Tyr Phe Glu Tyr Lys  Pro Val Asn 
    1130                 1135                 1140             


Val Thr  Gln Ser Leu Lys Gln  Leu Phe Asp Glu Ala  Gly Ile Asp 
    1145                 1150                 1155             


Tyr Gln  Ala Gly Ala Asp Leu  Lys Glu Ala Ile Val  Thr Gly Lys 
    1160                 1165                 1170             


Asn Thr  Lys Leu Leu Lys Gly  Leu Gly Glu Gln Leu  Asn Ile Leu 
    1175                 1180                 1185             


Leu Ala  Met Arg Tyr Asn Asn  Gly Lys His Gly Asn  Glu Glu Lys 
    1190                 1195                 1200             


Asp Tyr  Ile Val Ser Pro Val  Lys Asn Asn Tyr Gly  Lys Phe Phe 
    1205                 1210                 1215             


Cys Thr  Leu Asp Gly Asp Ala  Ser Leu Pro Val Asp  Ala Asp Ala 
    1220                 1225                 1230             


Asn Gly  Ala Tyr Ala Ile Ala  Leu Lys Gly Leu Met  Leu Val Glu 
    1235                 1240                 1245             


Arg Met  Lys Ser Asn Lys Asp  Ile Lys Gly Arg Ile  Asp Tyr Phe 
    1250                 1255                 1260             


Ile Ser  Asn Asn Glu Trp Phe  Asn Tyr Leu Ile Ala  Lys Asn Thr 
    1265                 1270                 1275             


Leu Asn  Lys Ser Lys 
    1280             


<210>  2
<211>  3852
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ukcpf1

<400>  2
atgagtaaca tgcaacagta cgataatttt atcaatcatt atgcaataca gaaaacacta       60

cggtttgaac tgcaaccaat aggaaaaaca cgggagcata ttcaaaaaaa cgggattatt      120

gagcatgatg aagcgcttga gcaaaagtat cagattgtta aaaagattat tgatcgtttt      180

cacaggaaac acattgatga agcactttct ttagcagatt tttcaaagga tactgctatg      240

ttaaagcgat ttgaggaatt atattggaaa aagaacaaaa atgaaaatga aaaaaatgaa      300

tttgtaaaaa tacaatctga tttgcgcaaa agggttgttt cttttttaga aggtaaagta      360

gaaggtgatg caaggtttgc aaaggtacag caacggtacg gtatactatt tgatgcaaaa      420

atatttaaag acaaagaatt tatatcaaca gcctgtgatg atattgaaaa agatgctata      480

gaagctttta aacgatttgc tacctatttt acaggtttcc atgaaaatcg taaaaacatg      540

tattccgctg atgaagaatc tactgccatt gcctatagag taatcaatga aaaccttccg      600

cgttttttag aaaataaagc aaggtttgaa aagatacaac acacagttga tagtaaaacg      660

cttaacgaga tagcaacaga acttaaacca gtattggaaa agaataagct tgaaaccata      720

tttacgctga actattttca aaatacacta tcgcaggctg gaattaccta ttataacacc      780

atccttggtg gtaaaactaa agaaaatggc gaaaaggttc agggcttaaa tgaaattata      840

aatcttttta atcagaaaaa taaggatact atgctgccat tattaaaacc actgtataag      900

caaattttaa gtgaagagta ttccacatca tttaccattt ctgcttttga aaaagataat      960

gatgttttgc aggcaatagg ttctttttgt aatgattgta ttttttatgc aaaaaataat     1020

gtcaatggca aggcatacaa ccttttgcaa acggtacagg cattttgtaa cagtattgat     1080

acctataacg ataacaggct tgatggtttg catatagaaa gaaaaaacct tgctaccctt     1140

tcacatcagg tgtatggcga atggaatata ctgcgggatg cattacagat acactatgaa     1200

gcgtatgaac aaaaggataa tggcaataat aataactatc tggaaagcaa aaccttttcg     1260

tggaaagcat taaaagatgc attaacaaca tataaatcac tggtggagga agcacaggat     1320

attgatgaaa atggatttat agcttacttc aaggacatga aatttaaaga agaaattgat     1380

ggtaaaacaa ccagtattga tttaattgaa aatatacaaa cccggtataa aagtattgaa     1440

accatattgc aggaagaccg caacaacaaa aacaatcttc atcaggaaaa ggaaaaggta     1500

gctacaataa agggctttct tgattcggta aaatacctgc aatggttttt aaaccttatg     1560

tatatagcaa gccctgttga tgataaagat tatgattttt acaatgagct tgagatgtac     1620

catgacacat tgttaccact tactacactg tataataaag tacgcaacta tatgacacgc     1680

aaaccatatt ccgttgaaaa gtttaaattg acctttgaaa aatcaactct tcttgatgga     1740

tgggataaaa acaaggaacg agccaatctt ggtgttatac taaggaaggg taataattat     1800

tatcttggca ttatgaacaa aaaatacaat gatatatttg atagtatccc cggacttact     1860

accactgact attgtgaaaa gatgaactat aaattgctgc ctggccccaa taaaatgttg     1920

cctaaagtat ttttttcaaa aaaaggtgta cagttttaca aaccttcaca ggaaatcatc     1980

cgtttgtata ataataagga atttaaaaag ggagatacct ttaataaaaa tagtttgcac     2040

aaacttatta atttttataa agaatccatt gcaaaaactg aagattggag tgtattccag     2100

tttaaattta aaaacacaaa cgattatgcc gatataagcc agttttataa agatgttgaa     2160

agacagggct ataaaattag ttttgataag attgattggg aatatatact attgctggta     2220

gatgaaggaa aattatttct tttcaagatt tacaataagg acttttcacc ctatagcaag     2280

ggtaagccta acctgcatac catttactgg aaaaatattt tttcccatga taatttaaac     2340

aatgttgtgt ataagcttaa tggtgaagcc gaagtttttt accggaagaa atccatagag     2400

tatccagaag aaatactaca aaaaggtcat catgtcaatg agttaaagga taaatttaag     2460

taccctatta ttaaagataa acgctatgcc gaagacaaat ttcttttcca cgttcccatt     2520

accatgaact ttttatcaaa aggggaacct aatataaatc aaagggttca gcaatatatt     2580

gcatctacta gtgaagatta tcatattatt ggtattgacc ggggtgaaag aaatttactg     2640

tatctgtcgc tcatagatgc aacaggcaaa ataataaaac aactatcgct caataccatt     2700

aaaaatgaaa attttaacac aacaatagat taccatgcaa aattagatga aaaagaaaag     2760

aaaagagaag aagcaagaaa aaattgggat gtcatagaaa atattaaaga gcttaaagaa     2820

ggatacctgt cacaggttgt tcatcagatt gcaaaactta tggttgaata taaagcaatc     2880

ctggtgatgg aggatttgaa taccggtttt aagcgtggaa ggtttaaagt ggaaaaacag     2940

gtatatcaaa aatttgaaaa aatgatgata gataaattaa attaccttgt gttgaaggac     3000

aggcaggcta cacagccagg tggaagctta aaagcatatc aacttgcatc gtcattggaa     3060

tcgtttaaaa aacttggtaa acagtgtggc atgatttttt atgttcctgc agtgtataca     3120

agcaagattg atccaacaac tggtttttac aactttttaa gggtagatgt ttcaacattg     3180

aacagcgcgc acagtttttt taacaggttt aatgcaattg tttataataa tgagcaggat     3240

tattttgaat ttcattgcac ctacaaaaat tttgtttctg aaccgtcatt gcaaaaaaat     3300

gtaaaaagca gcaaaatgca cgagtataac aatttaaaag atactacatg ggtactatgc     3360

tctactcatc atgagcgcta taaaaagttt aaaaataaaa gcggttattt tgagtataaa     3420

ccagttaatg taacccaaag cttaaagcag ctttttgacg aggctggtat tgattatcaa     3480

gcaggggctg acttaaagga agcgatagta accggtaaaa atactaaact attaaagggt     3540

ctgggcgagc agttgaacat actattagcc atgcgatata acaatggtaa acatgggaat     3600

gaagaaaaag attatattgt ttctccagta aaaaataatt atggaaaatt tttctgcacg     3660

cttgatggtg atgcatcatt acctgttgac gccgatgcca atggtgcgta tgccatagca     3720

ttgaaagggc ttatgcttgt tgaaaggatg aaaagcaata aagatataaa agggaggata     3780

gattatttta tttcaaataa tgagtggttt aactatttaa ttgcaaagaa cacattaaat     3840

aaatcaaaat aa                                                         3852


<210>  3
<211>  18
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-DR

<400>  3
auuucuacua uuguagau                                                     18


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gTGW6-1

<400>  4
actacaaaac cggcaacctg tac                                               23


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gTGW6-2

<400>  5
tttcaccgac agcagcatga act                                               23


<210>  6
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gTGW6-3

<400>  6
ttgacctgcc aggctatcct gat                                               23


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gTGW6-4

<400>  7
ggtccggata gtcacttggt tgc                                               23


<210>  8
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gTGW6-5

<400>  8
cgtgtagctg gggctgtacg tgt                                               23


<210>  9
<211>  52
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  N-B-i3g1-ssDNA0

<400>  9
cgacattccg aagaacgctg aagcgctggg ggcaaattgt gcaatttgcg gc               52


<210>  10
<211>  43
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  gRNA-trans

<400>  10
agagaaugug ugcauaguca caccccccag cgcuucagcg uuc                         43


