                               SEQUENCE LISTING

<110> THE GENERAL HOSPITAL CORPORATION
 
<120> TEMPLATE ASSEMBLY BY PROXIMITY-ENHANCED REACTIVITY VIA METABOLIC
      LABELING

<130> 29539-0533WO1

<140> PCT/US2021/058314
<141> 2021-11-05

<150> 63/111,401
<151> 2020-11-09

<160> 84    

<170> PatentIn version 3.5

<210> 1
<211> 14
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 1
Lys Leu Ala Lys Leu Ala Lys Lys Leu Ala Lys Leu Ala Lys 
1               5                   10                  


<210> 2
<211> 47
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 2
Cys Ser Gly Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys 
1               5                   10                  15      


Val Lys Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn 
            20                  25                  30          


Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45          


<210> 3
<211> 49
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 3
Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys 
1               5                   10                  15      


Thr Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu 
            20                  25                  30          


Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro Ser Gly Gly 
        35                  40                  45              


Cys 
    


<210> 4
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 4
Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu 
1               5                   10                  15      


Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys 
            20                  25                  30          


Glu Lys Glu Lys Leu Glu Gly Ala Pro 
        35                  40      


<210> 5
<211> 47
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 5
Cys Ser Gly Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys 
1               5                   10                  15      


Val Lys Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn 
            20                  25                  30          


Met Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45          


<210> 6
<211> 49
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys 
1               5                   10                  15      


Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn Met Leu 
            20                  25                  30          


Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro Ser Gly Gly 
        35                  40                  45              


Cys 
    


<210> 7
<211> 60
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
actcgagacg tctccttgtc tttgcttttc ttcaggacac agtggcgaga cgtctcgagt       60


<210> 8
<211> 51
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 8
actcgagacg tctccttcct gcccctcctc ctgctccgag acgtctcgag t                51


<210> 9
<211> 11
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
agctctcgag t                                                            11


<210> 10
<211> 12
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
gacgtctcga gt                                                           12


<210> 11
<211> 29
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 11
Ala Leu Lys Lys Glu Leu Gln Ala Asn Lys Lys Glu Leu Ala Gln Leu 
1               5                   10                  15      


Lys Trp Glu Leu Gln Ala Leu Lys Lys Glu Leu Ala Gln 
            20                  25                  


<210> 12
<211> 30
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Glu Gln Leu Glu Lys Lys Leu Gln Ala Leu Glu Lys Lys Leu Ala Gln 
1               5                   10                  15      


Leu Glu Trp Lys Asn Gln Ala Leu Glu Lys Lys Leu Ala Gln 
            20                  25                  30  


<210> 13
<211> 46
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Cys Ser Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val 
1               5                   10                  15      


Lys Ser Phe Lys Ala Gln Asn Ser Glu Asn Ala Ser Thr Ala Asn Met 
            20                  25                  30          


Leu Arg Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro 
        35                  40                  45      


<210> 14
<211> 43
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 14
Ser Gly Ala Ser Arg Glu Leu Thr Asp Thr Leu Gln Ala Glu Thr Asp 
1               5                   10                  15      


Gln Leu Glu Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu 
            20                  25                  30          


Leu Lys Glu Lys Glu Lys Leu Glu Gly Ala Pro 
        35                  40              


<210> 15
<211> 97
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 15
Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe Pro 
1               5                   10                  15      


Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
            20                  25                  30          


Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
        35                  40                  45              


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
    50                  55                  60                  


Gln Met Cys Pro Asp Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile 
65                  70                  75                  80  


Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu 
                85                  90                  95      


Glu 
    


<210> 16
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 16
Met Gly Val Gln Val Glu Thr Ile Ser Pro Gly Asp Gly Arg Thr Phe 
1               5                   10                  15      


Pro Lys Arg Gly Gln Thr Cys Val Val His Tyr Thr Gly Met Leu Glu 
            20                  25                  30          


Asp Gly Lys Lys Val Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys 
        35                  40                  45              


Phe Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val 
    50                  55                  60                  


Ala Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp 
65                  70                  75                  80  


Tyr Ala Tyr Gly Ala Thr Gly His Pro Gly Ile Ile Pro Pro His Ala 
                85                  90                  95      


Thr Leu Val Phe Asp Val Glu Leu Leu Lys Leu Glu 
            100                 105             


<210> 17
<211> 10
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 17
Ala Gly Ser Ser Ala Ala Gly Ser Gly Ser 
1               5                   10  


<210> 18
<211> 18
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 18
Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly 
        


<210> 19
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 19
Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Gly 
            20          


<210> 20
<211> 26
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 20
Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly 
            20                  25      


<210> 21
<211> 15
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 21
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15  


<210> 22
<211> 20
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 22
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Gly 
            20  


<210> 23
<211> 25
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 23
Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15      


Gly Gly Gly Gly Ser Gly Gly Gly Gly 
            20                  25  


<210> 24
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 24
Ser Gly Gly Gly Ser 
1               5   


<210> 25
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 25
Ser Gly Ser Gly 
1               


<210> 26
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 26
Ser Gly Gly Gly Gly Ser 
1               5       


<210> 27
<211> 5
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 27
Ser Gly Ser Gly Gly 
1               5   


<210> 28
<211> 156
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      superfolder GFP sequence

<400> 28
Met Arg Lys Gly Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu 
1               5                   10                  15      


Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu Gly 
            20                  25                  30          


Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr 
        35                  40                  45              


Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr 
    50                  55                  60                  


Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln His 
65                  70                  75                  80  


Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr 
                85                  90                  95      


Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val Lys 
            100                 105                 110         


Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp 
        115                 120                 125             


Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Phe 
    130                 135                 140                 


Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155     


<210> 29
<211> 81
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      superfolder GFP sequence

<400> 29
Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp 
1               5                   10                  15      


Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly 
            20                  25                  30          


Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser 
        35                  40                  45              


Val Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu 
    50                  55                  60                  


Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr 
65                  70                  75                  80  


Lys 
    


<210> 30
<211> 229
<212> PRT
<213> Renilla reniformis

<400> 30
Met Ala Ser Lys Val Tyr Asp Pro Glu Gln Arg Lys Arg Met Ile Thr 
1               5                   10                  15      


Gly Pro Gln Trp Trp Ala Arg Cys Lys Gln Met Asn Val Leu Asp Ser 
            20                  25                  30          


Phe Ile Asn Tyr Tyr Asp Ser Glu Lys His Ala Glu Asn Ala Val Ile 
        35                  40                  45              


Phe Leu His Gly Asn Ala Ala Ser Ser Tyr Leu Trp Arg His Val Val 
    50                  55                  60                  


Pro His Ile Glu Pro Val Ala Arg Cys Ile Ile Pro Asp Leu Ile Gly 
65                  70                  75                  80  


Met Gly Lys Ser Gly Lys Ser Gly Asn Gly Ser Tyr Arg Leu Leu Asp 
                85                  90                  95      


His Tyr Lys Tyr Leu Thr Ala Trp Phe Glu Leu Leu Asn Leu Pro Lys 
            100                 105                 110         


Lys Ile Ile Phe Val Gly His Asp Trp Gly Ala Cys Leu Ala Phe His 
        115                 120                 125             


Tyr Ser Tyr Glu His Gln Asp Lys Ile Lys Ala Ile Val His Ala Glu 
    130                 135                 140                 


Ser Val Val Asp Val Ile Glu Ser Trp Asp Glu Trp Pro Asp Ile Glu 
145                 150                 155                 160 


Glu Asp Ile Ala Leu Ile Lys Ser Glu Glu Gly Glu Lys Met Val Leu 
                165                 170                 175     


Glu Asn Asn Phe Phe Val Glu Thr Met Leu Pro Ser Lys Ile Met Arg 
            180                 185                 190         


Lys Leu Glu Pro Glu Glu Phe Ala Ala Tyr Leu Glu Pro Phe Lys Glu 
        195                 200                 205             


Lys Gly Glu Val Arg Arg Pro Thr Leu Ser Trp Pro Arg Glu Ile Pro 
    210                 215                 220                 


Leu Val Lys Gly Gly 
225                 


<210> 31
<211> 82
<212> PRT
<213> Renilla reniformis

<400> 31
Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg 
1               5                   10                  15      


Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe 
            20                  25                  30          


Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu 
        35                  40                  45              


Phe Val Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp 
    50                  55                  60                  


Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn 
65                  70                  75                  80  


Glu Gln 
        


<210> 32
<211> 93
<212> PRT
<213> Gaussia princeps

<400> 32
Met Lys Pro Thr Glu Asn Asn Glu Asp Phe Asn Ile Val Ala Val Ala 
1               5                   10                  15      


Ser Asn Phe Ala Thr Thr Asp Leu Asp Ala Asp Arg Gly Lys Leu Pro 
            20                  25                  30          


Gly Lys Lys Leu Pro Leu Glu Val Leu Lys Glu Met Glu Ala Asn Ala 
        35                  40                  45              


Arg Lys Ala Gly Cys Thr Arg Gly Cys Leu Ile Cys Leu Ser His Ile 
    50                  55                  60                  


Lys Cys Thr Pro Lys Met Lys Lys Phe Ile Pro Gly Arg Cys His Thr 
65                  70                  75                  80  


Tyr Glu Gly Asp Lys Glu Ser Ala Gln Gly Gly Ile Gly 
                85                  90              


<210> 33
<211> 76
<212> PRT
<213> Gaussia princeps

<400> 33
Glu Ala Ile Val Asp Ile Pro Glu Ile Pro Gly Phe Lys Asp Leu Glu 
1               5                   10                  15      


Pro Met Glu Gln Phe Ile Ala Gln Val Asp Leu Cys Val Asp Cys Thr 
            20                  25                  30          


Thr Gly Cys Leu Lys Gly Leu Ala Asn Val Gln Cys Ser Asp Leu Leu 
        35                  40                  45              


Lys Lys Trp Leu Pro Gln Arg Cys Ala Thr Phe Ala Ser Lys Ile Gln 
    50                  55                  60                  


Gly Gln Val Asp Lys Ile Lys Gly Ala Gly Gly Asp 
65                  70                  75      


<210> 34
<211> 88
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 34
Ala Pro Ile Val Thr Cys Arg Lys Leu Asp Gly Arg Glu Lys Pro Phe 
1               5                   10                  15      


Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly Leu 
            20                  25                  30          


Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His 
        35                  40                  45              


Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp 
    50                  55                  60                  


Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp 
65                  70                  75                  80  


Val Lys Thr Ser Gln Gln Lys Gly 
                85              


<210> 35
<211> 41
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 35
Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val 
1               5                   10                  15      


Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln 
            20                  25                  30          


Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40      


<210> 36
<211> 12
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 36
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly 
1               5                   10          


<210> 37
<211> 118
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 37
Arg Glu Lys Pro Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala 
1               5                   10                  15      


Arg Lys Ala Gly Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr 
            20                  25                  30          


Phe Ala Gly Asp His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala 
        35                  40                  45              


Asp Ala Ile Leu Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala 
    50                  55                  60                  


Glu Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr 
65                  70                  75                  80  


Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys 
                85                  90                  95      


Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu 
            100                 105                 110         


Phe Phe Glu Lys Cys Asp 
        115             


<210> 38
<211> 37
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 38
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys 
        35          


<210> 39
<211> 93
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 39
Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His Ile Arg Trp Gly 
1               5                   10                  15      


Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp Glu Tyr Pro Ile 
            20                  25                  30          


Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp Val Lys Thr Ser 
        35                  40                  45              


Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser 
    50                  55                  60                  


Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp 
65                  70                  75                  80  


Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
                85                  90              


<210> 40
<211> 61
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 40
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp 
    50                  55                  60      


<210> 41
<211> 69
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 41
Ala Ile Leu Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu 
1               5                   10                  15      


Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro 
            20                  25                  30          


Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly 
        35                  40                  45              


Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe 
    50                  55                  60                  


Phe Glu Lys Cys Asp 
65                  


<210> 42
<211> 73
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 42
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly 
65                  70              


<210> 43
<211> 57
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 43
Lys Asn Ala Glu Trp Ala Lys Asp Val Lys Thr Ser Gln Gln Lys Gly 
1               5                   10                  15      


Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala Val 
            20                  25                  30          


Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn Gln 
        35                  40                  45              


Gly Lys Glu Phe Phe Glu Lys Cys Asp 
    50                  55          


<210> 44
<211> 81
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 44
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  


Asp 
    


<210> 45
<211> 49
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 45
Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val 
1               5                   10                  15      


Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His 
            20                  25                  30          


Ser Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys 
        35                  40                  45              


Asp 
    


<210> 46
<211> 86
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 46
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  


Asp Val Lys Thr Ser Gln 
                85      


<210> 47
<211> 44
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 47
Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg 
1               5                   10                  15      


Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys 
            20                  25                  30          


Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40                  


<210> 48
<211> 103
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 48
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  


Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val 
                85                  90                  95      


Val Tyr Ala Asn Ser Arg Gly 
            100             


<210> 49
<211> 27
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 49
Ala Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn 
1               5                   10                  15      


Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
            20                  25          


<210> 50
<211> 119
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 50
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  


Asp Val Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val 
                85                  90                  95      


Val Tyr Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr 
            100                 105                 110         


His Ser Lys Val Asp Lys Asn 
        115                 


<210> 51
<211> 11
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 51
Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
1               5                   10      


<210> 52
<211> 34
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 52
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr 
        


<210> 53
<211> 96
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 53
Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp His Ile 
1               5                   10                  15      


Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu Trp Glu 
            20                  25                  30          


Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys Asp Val 
        35                  40                  45              


Lys Thr Ser Gln Gln Lys Gly Gly Pro Thr Pro Ile Arg Val Val Tyr 
    50                  55                  60                  


Ala Asn Ser Arg Gly Ala Val Gln Tyr Cys Gly Val Met Thr His Ser 
65                  70                  75                  80  


Lys Val Asp Lys Asn Asn Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
                85                  90                  95      


<210> 54
<211> 34
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 54
aagccactgt gtcctgaaga aaagcaaaga catc                                   34


<210> 55
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (7)..(7)
<223> D-Phenylalanine

<400> 55
Ser Tyr Ser Met Glu His Phe Arg Trp Gly Lys Pro Val Gly Gly Gly 
1               5                   10                  15      


Ser Ser Gly Gly Gly Cys 
            20          


<210> 56
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (4)..(4)
<223> Norleucine

<220>
<221> MOD_RES
<222> (7)..(7)
<223> D-Phenylalanine

<400> 56
Ser Tyr Ser Xaa Glu His Phe Arg Trp Gly Lys Pro Val Gly Gly Gly 
1               5                   10                  15      


Ser Ser Gly Gly Gly Cys 
            20          


<210> 57
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (17)..(17)
<223> D-Phenylalanine

<400> 57
Cys Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser Tyr Ser Met Glu His 
1               5                   10                  15      


Phe Arg Trp Gly Lys Pro Val 
            20              


<210> 58
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (14)..(14)
<223> Norleucine

<220>
<221> MOD_RES
<222> (17)..(17)
<223> D-Phenylalanine

<400> 58
Cys Ser Gly Gly Gly Ser Ser Gly Gly Gly Ser Tyr Ser Xaa Glu His 
1               5                   10                  15      


Phe Arg Trp Gly Lys Pro Val 
            20              


<210> 59
<211> 90
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 59
Ala Pro Ile Val Thr Cys Arg Pro Lys Leu Asp Gly Arg Glu Lys Pro 
1               5                   10                  15      


Phe Lys Val Asp Val Ala Thr Ala Gln Ala Gln Ala Arg Lys Ala Gly 
            20                  25                  30          


Leu Thr Thr Gly Lys Ser Gly Asp Pro His Arg Tyr Phe Ala Gly Asp 
        35                  40                  45              


His Ile Arg Trp Gly Val Asn Asn Cys Asp Lys Ala Asp Ala Ile Leu 
    50                  55                  60                  


Trp Glu Tyr Pro Ile Tyr Trp Val Gly Lys Asn Ala Glu Trp Ala Lys 
65                  70                  75                  80  


Asp Val Lys Thr Ser Gln Gln Lys Gly Cys 
                85                  90  


<210> 60
<211> 42
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 60
Cys Gly Pro Thr Pro Ile Arg Val Val Tyr Ala Asn Ser Arg Gly Ala 
1               5                   10                  15      


Val Gln Tyr Cys Gly Val Met Thr His Ser Lys Val Asp Lys Asn Asn 
            20                  25                  30          


Gln Gly Lys Glu Phe Phe Glu Lys Cys Asp 
        35                  40          


<210> 61
<211> 4
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      Factor Xa protease cleavage site sequence

<400> 61
Ile Glu Gly Arg 
1               


<210> 62
<211> 9
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 62
Gly Ser Gly Ser Gly His His His His 
1               5                   


<210> 63
<211> 34
<212> PRT
<213> Saccharomyces cerevisiae

<400> 63
Met Gln Ile Phe Val Lys Thr Leu Thr Gly Lys Thr Ile Thr Leu Glu 
1               5                   10                  15      


Val Glu Ser Ser Asp Thr Ile Asp Asn Val Lys Ser Lys Ile Gln Asp 
            20                  25                  30          


Lys Glu 
        


<210> 64
<211> 42
<212> PRT
<213> Saccharomyces cerevisiae

<400> 64
Gly Ile Pro Pro Asp Gln Gln Arg Leu Ile Phe Ala Gly Lys Gln Leu 
1               5                   10                  15      


Glu Asp Gly Arg Thr Leu Ser Asp Tyr Asn Ile Gln Lys Glu Ser Thr 
            20                  25                  30          


Leu His Leu Val Leu Arg Leu Arg Gly Gly 
        35                  40          


<210> 65
<211> 157
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      superfolder GFP sequence

<400> 65
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155         


<210> 66
<211> 40
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 66
uuuuuccugu guccugaaga aagcaaagac aucuggacaa                             40


<210> 67
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 67
ctttcttcag gacacagagg tagcgtctca ggtgggaagt tcggattgcc agtaggtg         58


<210> 68
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 68
cactactgtg ctcctctcac tgcactcttg ctcactccac ttttgtccag atgtctttg        59


<210> 69
<211> 117
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 69
cactactgtg ctcctctcac tgcactcttg ctcactccac ttttgtccag atgtctttgc       60

tttcttcagg acacagaggt agcgtctcag gtgggaagtt cggattgcca gtaggtg         117


<210> 70
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 70
cacctactgg caatccgaac t                                                 21


<210> 71
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 71
cactactgtg ctcctctcac t                                                 21


<210> 72
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      probe

<400> 72
tccagatgtc tttgcttttc ttcaggacac a                                      31


<210> 73
<211> 32
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 73
cttacctaat gctgtttata tcatcgttct gt                                     32


<210> 74
<211> 58
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 74
ctttcttcag gacacagagg tagcgtctca ggtgggaagt tcggattgcc agtaggtg         58


<210> 75
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 75
cactactgtg ctcctctcac tgcactcttg ctcactccac ttttgtccag atgtctttg        59


<210> 76
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 76
tcccacctga gacgctacct                                                   20


<210> 77
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 77
gcactcttgc tcactccact t                                                 21


<210> 78
<211> 48
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 78
Gly Ala Ser Leu Glu Arg Ile Ala Arg Leu Glu Glu Lys Val Lys Thr 
1               5                   10                  15      


Leu Lys Ala Gln Asn Ser Glu Leu Ala Ser Thr Ala Asn Met Leu Arg 
            20                  25                  30          


Glu Gln Val Ala Gln Leu Lys Gln Lys Gly Ala Pro Ser Gly Gly Cys 
        35                  40                  45              


<210> 79
<211> 40
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 79
Ala Ser Arg Glu Thr Asp Thr Leu Gln Ala Glu Thr Asp Gln Leu Glu 
1               5                   10                  15      


Asp Glu Lys Ser Ala Leu Gln Thr Glu Ile Ala Asn Leu Leu Lys Glu 
            20                  25                  30          


Lys Glu Lys Leu Glu Gly Ala Pro 
        35                  40  


<210> 80
<211> 157
<212> PRT
<213> Unknown

<220>
<223> Description of Unknown: 
      superfolder GFP sequence

<400> 80
Met Arg Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val 
1               5                   10                  15      


Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu 
            20                  25                  30          


Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile Cys 
        35                  40                  45              


Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu 
    50                  55                  60                  


Thr Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Gln 
65                  70                  75                  80  


His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg 
                85                  90                  95      


Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu Val 
            100                 105                 110         


Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile 
        115                 120                 125             


Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn 
    130                 135                 140                 


Phe Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln 
145                 150                 155         


<210> 81
<211> 83
<212> PRT
<213> Renilla reniformis

<400> 81
Lys Pro Asp Val Val Gln Ile Val Arg Asn Tyr Asn Ala Tyr Leu Arg 
1               5                   10                  15      


Ala Ser Asp Asp Leu Pro Lys Met Phe Ile Glu Ser Asp Pro Gly Phe 
            20                  25                  30          


Phe Ser Asn Ala Ile Val Glu Gly Ala Lys Lys Phe Pro Asn Thr Glu 
        35                  40                  45              


Phe Val Lys Val Lys Gly Leu His Phe Ser Gln Glu Asp Ala Pro Asp 
    50                  55                  60                  


Glu Met Gly Lys Tyr Ile Lys Ser Phe Val Glu Arg Val Leu Lys Asn 
65                  70                  75                  80  


Glu Gln Glx 
            


<210> 82
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (6)..(6)
<223> D-Methionine

<400> 82
Trp Lys Tyr Met Val Met 
1               5       


<210> 83
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 83
His His His His His His 
1               5       


<210> 84
<211> 4
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      4xHis tag

<400> 84
His His His His 
1               


