                         SEQUENCE LISTING

<110>  YALE UNIVERSITY
       Chen, Lieping
       Zheng, Linghua
 
<120>  METHODS AND AGENTS FOR MODULATING NOVEL IMMUNOLOGICAL INTERACTION

<130>  047162-5270-00WO

<160>  27    

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine Ab 3D9 VH

<400>  1

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Lys 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Glu Ala Ser Gly Phe Thr Val Arg Asn Tyr 
            20                  25                  30          


Tyr Met Asn Trp Val Arg Gln Ala Pro Gly Lys Ala Leu Glu Trp Val 
        35                  40                  45              


Ala Asp Thr Ser Gly Gly Ser Ser Asp Ile Lys Tyr Ala Asp Val Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Leu Leu Phe 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Ser Glu Asp Thr Ala Met Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Ser Arg Leu Gly Arg Gly Asp Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Met Val Thr Val Ser Ser 
        115                 120 


<210>  2
<211>  360
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine Ab 3D9 VH

<400>  2
gaggtgcagc tggtggagtc tgggggaggc ttggtgcagc ctggaaagtc cctgaaactc       60

tcctgtgagg cctctggatt caccgtcagg aactattaca tgaactgggt ccgccaggct      120

ccagggaagg cgctggagtg ggtcgcagac actagtggtg gtagtagtga tatcaaatat      180

gccgacgttg tgaagggccg gttcaccatc tccagagaca atgccaagaa cctactgttc      240

ctacaaatga acaatctcaa gtctgaggac acagccatgt attactgtgc aagatctcgg      300

ctgggacggg gggactactt tgattactgg ggccaaggaa ccatggtcac cgtctcctca      360


<210>  3
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Murine Ab 3D9 VL

<400>  3

Gln Phe Val Leu Thr Gln Pro Lys Ser Val Ser Glu Ser Leu Gly Arg 
1               5                   10                  15      


Thr Val Thr Ile Ser Cys Lys Arg Ser Ser Gly Asn Ile Gly Asp Tyr 
            20                  25                  30          


Tyr Val Ser Trp Tyr Gln Gln His Phe Gly Ser Ser Pro Thr Thr Val 
        35                  40                  45              


Ile Tyr Asp Asp His Lys Arg Pro Ser Gly Val Pro Glu Arg Phe Ser 
    50                  55                  60                  


Gly Ser Ile Asp Ser Ser Ser Asn Ser Ala Ser Leu Thr Ile Thr Asp 
65                  70                  75                  80  


Leu Gln Thr Asp Asp Glu Ala Glu Tyr Phe Cys Phe Ser Tyr Asp Ser 
                85                  90                  95      


Asn Asn Leu Val Phe Gly Ser Gly Thr Gln Leu Thr Val Leu 
            100                 105                 110 


<210>  4
<211>  331
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Murine Ab 3D9 VL

<400>  4
cagtttgtgc taactcagcc aaagtctgtg tcagagtctc tagggcgaac agtcaccatc       60

tcctgcaagc gcagcagtgg caacattggg gactactatg tgagctggta ccagcagcac      120

tttggaagct cacccacaac tgtgatctat gatgatcata aaaggccatc tggggtacct      180

gaaaggttct ctggctccat tgacagctcc tccaactcag cctcgctgac catcactgat      240

ctgcagactg acgatgaggc tgaatacttc tgtttctcgt atgatagtaa caatcttgtt      300

tttggcagcg gaacccagct taccgtccta g                                     331


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 HC-CDR1

<400>  5

Gly Phe Thr Val Arg Asn Tyr Tyr 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 HC-CDR2

<400>  6

Thr Ser Gly Gly Ser Ser Asp Ile 
1               5               


<210>  7
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 HC-CDR3

<400>  7

Ala Arg Ser Arg Leu Gly Arg Gly Asp Tyr Phe Asp Tyr 
1               5                   10              


<210>  8
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 LC-CDR1

<400>  8

Ser Gly Asn Ile Gly Asp Tyr Tyr 
1               5               


<210>  9
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 LC-CDR2

<400>  9

Asp Asp His 
1           


<210>  10
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3D9 LC-CDR3

<400>  10

Phe Ser Tyr Asp Ser Asn Asn Leu Val 
1               5                   


<210>  11
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  human CD8? sequence (UniProtKB - P01732)

<400>  11

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ser Gln Phe Arg Val Ser Pro Leu Asp Arg Thr 
            20                  25                  30          


Trp Asn Leu Gly Glu Thr Val Glu Leu Lys Cys Gln Val Leu Leu Ser 
        35                  40                  45              


Asn Pro Thr Ser Gly Cys Ser Trp Leu Phe Gln Pro Arg Gly Ala Ala 
    50                  55                  60                  


Ala Ser Pro Thr Phe Leu Leu Tyr Leu Ser Gln Asn Lys Pro Lys Ala 
65                  70                  75                  80  


Ala Glu Gly Leu Asp Thr Gln Arg Phe Ser Gly Lys Arg Leu Gly Asp 
                85                  90                  95      


Thr Phe Val Leu Thr Leu Ser Asp Phe Arg Arg Glu Asn Glu Gly Tyr 
            100                 105                 110         


Tyr Phe Cys Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe 
        115                 120                 125             


Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg 
    130                 135                 140                 


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
145                 150                 155                 160 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
                165                 170                 175     


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
            180                 185                 190         


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His 
        195                 200                 205             


Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Lys Ser 
    210                 215                 220                 


Gly Asp Lys Pro Ser Leu Ser Ala Arg Tyr Val 
225                 230                 235 


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' guide RNA

<400>  12
ctagaatgaa gcagtaacgt                                                   20


<210>  13
<211>  166
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' Homology Directed Repair (HDR) and inserted loxp oligo

<400>  13
ggcagaactc agatgtgggg tagagactgc caggcaacct gtctccaact agaatgaagc       60

agtaagtcga cataacttcg tataatgtat gctatacgaa gttatcgtgg gaggcacacc      120

ctgtatgggg ttccaagtgg aaaaccccac taagtacaag ccacat                     166


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' guide RNA

<400>  14
tacaggtgat tacaccaacc                                                   20


<210>  15
<211>  166
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' Homology Directed Repair (HDR) and inserted loxp (capitalized)
       oligo

<400>  15
agatttgcct taatcccacc tcacaaacca ttcactcctc tgagtctata caggtgatta       60

caccaataac ttcgtataat gtatgctata cgaagttatc tcgagaccag gtcaacagat      120

tcccagagac actcacatct gcatttacaa tgcctctccc ttccca                     166


<210>  16
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' loxp insertion genotyping forward primer

<400>  16
gggcagaact cagatgtggg gtag                                              24


<210>  17
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' loxp insertion genotyping reverse primer

<400>  17
ttgggccttt gactttctct gttg                                              24


<210>  18
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' loxp insertion genotyping forward primer

<400>  18
agcagccaaa tcagcagtta gcac                                              24


<210>  19
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' loxp insertion genotyping reverse primer

<400>  19
ttgggaaggg agaggcattg ta                                                22


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  5' guide RNA

<400>  20
gtcactgtcc aagaagccca                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3' guide RNA

<400>  21
cctcaagtga agcttccgca                                                   20


<210>  22
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primers: primer 1F

<400>  22
gcctctgccc caacctttca a                                                 21


<210>  23
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primers: primer 1R

<400>  23
ctgctttctc cctggcgttc tct                                               23


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primers: primer 2F

<400>  24
aacgaaggcg gcgacgacaa                                                   20


<210>  25
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Genotyping primers: primer 2R

<400>  25
tcccccatca ccacagagtt agg                                               23


<210>  26
<211>  234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain

<400>  26

Met Thr Trp Thr Pro Leu Leu Leu Ile Phe Leu His His Leu Met Gly 
1               5                   10                  15      


Ser Cys Ala Gln Phe Val Leu Thr Gln Pro Lys Ser Val Ser Glu Ser 
            20                  25                  30          


Leu Gly Arg Thr Val Thr Ile Ser Cys Lys Arg Ser Ser Gly Asn Ile 
        35                  40                  45              


Gly Asp Tyr Tyr Val Ser Trp Tyr Gln Gln His Phe Gly Ser Ser Pro 
    50                  55                  60                  


Thr Thr Val Ile Tyr Asp Asp His Lys Arg Pro Ser Gly Val Pro Glu 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Ile Asp Ser Ser Ser Asn Ser Ala Ser Leu Thr 
                85                  90                  95      


Ile Thr Asp Leu Gln Thr Asp Asp Glu Ala Glu Tyr Phe Cys Phe Ser 
            100                 105                 110         


Tyr Asp Ser Asn Asn Leu Val Phe Gly Ser Gly Thr Gln Leu Thr Val 
        115                 120                 125             


Leu Gly Gly Pro Lys Ser Ser Pro Lys Val Thr Val Phe Pro Pro Ser 
    130                 135                 140                 


Pro Glu Glu Leu Arg Thr Asn Lys Ala Thr Leu Val Cys Leu Val Asn 
145                 150                 155                 160 


Asp Phe Tyr Pro Gly Ser Ala Thr Val Thr Trp Lys Ala Asn Gly Ala 
                165                 170                 175     


Thr Ile Asn Asp Gly Val Lys Thr Thr Lys Pro Ser Lys Gln Gly Gln 
            180                 185                 190         


Asn Tyr Met Thr Ser Ser Tyr Leu Ser Leu Thr Ala Asp Gln Trp Lys 
        195                 200                 205             


Ser His Asn Arg Val Ser Cys Gln Val Thr His Glu Gly Glu Thr Val 
    210                 215                 220                 


Glu Lys Ser Leu Ser Pro Ala Glu Cys Leu 
225                 230                 


<210>  27
<211>  258
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain

<400>  27

Met Asn Ser Gly Leu Lys Phe Val Phe Phe Val Leu Ile Leu Lys Gly 
1               5                   10                  15      


Val Gln Cys Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Lys Ser Leu Lys Leu Ser Cys Glu Ala Ser Gly Phe Thr Val 
        35                  40                  45              


Arg Asn Tyr Tyr Met Asn Trp Val Arg Gln Ala Pro Gly Lys Ala Leu 
    50                  55                  60                  


Glu Trp Val Ala Asp Thr Ser Gly Gly Ser Ser Asp Ile Lys Tyr Ala 
65                  70                  75                  80  


Asp Val Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn 
                85                  90                  95      


Leu Leu Phe Leu Gln Met Asn Asn Leu Lys Ser Glu Asp Thr Ala Met 
            100                 105                 110         


Tyr Tyr Cys Ala Arg Ser Arg Leu Gly Arg Gly Asp Tyr Phe Asp Tyr 
        115                 120                 125             


Trp Gly Gln Gly Thr Met Val Thr Val Ser Ser Ala Thr Thr Thr Ala 
    130                 135                 140                 


Pro Ser Val Tyr Pro Leu Ala Pro Ala Cys Asp Ser Thr Thr Ser Thr 
145                 150                 155                 160 


Thr Asn Thr Val Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu 
                165                 170                 175     


Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly Val His 
            180                 185                 190         


Thr Phe Pro Ser Val Leu His Ser Gly Leu Tyr Ser Leu Ser Ser Ser 
        195                 200                 205             


Val Thr Val Pro Ser Ser Thr Trp Pro Ser Gln Thr Val Thr Cys Asn 
    210                 215                 220                 


Val Ala His Pro Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro 
225                 230                 235                 240 


Gly Asp Gly Ser Gly Cys Gly Gly Gly Ser Pro His His His His His 
                245                 250                 255     


His Gly 
        


