                         SEQUENCE LISTING

<110>  WUGEN, Inc.
 
<120>  CHIMERIC ANTIGEN RECEPTOR CELL THERAPY

<130>  WGN0010-401-PC

<150>  63/109,076
<151>  2020-11-03

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha signal/leader peptide (variant 1)

<400>  1

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  2
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha signal/leader peptide (variant 2)

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala 
            


<210>  3
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha signal/leader peptide (variant 3)

<400>  3

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro 
1               5                   10      


<210>  4
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha signal/leader peptide (variant 4)

<400>  4

Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu 
1               5                   10          


<210>  5
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha signal/leader peptide (variant 5)

<400>  5

Leu Leu Leu Pro Leu Ala Leu Leu Leu His Ala Ala Arg Pro 
1               5                   10                  


<210>  6
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8-alpha hinge

<400>  6

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  7
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 Transmembrane (Tm) domain

<400>  7

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  8
<211>  235
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Surface glycoprotein CD8 alpha chain isoform 1 precursor 
       (NP_001139345.1)

<400>  8

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Ser Gln Phe Arg Val Ser Pro Leu Asp Arg Thr 
            20                  25                  30          


Trp Asn Leu Gly Glu Thr Val Glu Leu Lys Cys Gln Val Leu Leu Ser 
        35                  40                  45              


Asn Pro Thr Ser Gly Cys Ser Trp Leu Phe Gln Pro Arg Gly Ala Ala 
    50                  55                  60                  


Ala Ser Pro Thr Phe Leu Leu Tyr Leu Ser Gln Asn Lys Pro Lys Ala 
65                  70                  75                  80  


Ala Glu Gly Leu Asp Thr Gln Arg Phe Ser Gly Lys Arg Leu Gly Asp 
                85                  90                  95      


Thr Phe Val Leu Thr Leu Ser Asp Phe Arg Arg Glu Asn Glu Gly Tyr 
            100                 105                 110         


Tyr Phe Cys Ser Ala Leu Ser Asn Ser Ile Met Tyr Phe Ser His Phe 
        115                 120                 125             


Val Pro Val Phe Leu Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg 
    130                 135                 140                 


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
145                 150                 155                 160 


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
                165                 170                 175     


Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr 
            180                 185                 190         


Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Asn His 
        195                 200                 205             


Arg Asn Arg Arg Arg Val Cys Lys Cys Pro Arg Pro Val Val Lys Ser 
    210                 215                 220                 


Gly Asp Lys Pro Ser Leu Ser Ala Arg Tyr Val 
225                 230                 235 


<210>  9
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  4-1BB costimulatory domain

<400>  9

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  10
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 costimulatory domain

<400>  10

Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      


<210>  11
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD3 zeta

<400>  11

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  12
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A peptide

<400>  12

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  13
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)4 linker

<400>  13

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)3 linker

<400>  14

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)2 linker

<400>  15

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  16
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (GGGGS)1 linker

<400>  16

Gly Gly Gly Gly Ser 
1               5   


<210>  17
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  hCD34

<400>  17

Met Pro Arg Gly Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser 
1               5                   10                  15      


Gly Phe Met Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro 
            20                  25                  30          


Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu 
        35                  40                  45              


Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser 
    50                  55                  60                  


Gln His Gly Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys 
65                  70                  75                  80  


Phe Thr Ser Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser 
                85                  90                  95      


Ser Val Gln Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro 
            100                 105                 110         


Ala Asn Val Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro 
        115                 120                 125             


Gly Asn Val Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser 
    130                 135                 140                 


Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys 
145                 150                 155                 160 


Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly 
                165                 170                 175     


Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys 
            180                 185                 190         


Asp Arg Gly Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala 
        195                 200                 205             


Asp Ala Asp Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser 
    210                 215                 220                 


Glu Val Arg Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu 
225                 230                 235                 240 


Ile Ser Ser Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys 
                245                 250                 255     


Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln 
            260                 265                 270         


Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu 
        275                 280                 285             


Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser 
    290                 295                 300                 


Trp Ser Pro Ile 
305             


<210>  18
<211>  313
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TrhCD34

<400>  18

Val Gly Pro Phe Glu Ala Met Pro Arg Gly Trp Thr Ala Leu Cys Leu 
1               5                   10                  15      


Leu Ser Leu Leu Pro Ser Gly Phe Met Ser Leu Asp Asn Asn Gly Thr 
            20                  25                  30          


Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr 
        35                  40                  45              


Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr 
    50                  55                  60                  


Ser Leu His Pro Val Ser Gln His Gly Asn Glu Ala Thr Thr Asn Ile 
65                  70                  75                  80  


Thr Glu Thr Thr Val Lys Phe Thr Ser Thr Ser Val Ile Thr Ser Val 
                85                  90                  95      


Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser Gln Thr Ser Val Ile Ser 
            100                 105                 110         


Thr Val Phe Thr Thr Pro Ala Asn Val Ser Thr Pro Glu Thr Thr Leu 
        115                 120                 125             


Lys Pro Ser Leu Ser Pro Gly Asn Val Ser Asp Leu Ser Thr Thr Ser 
    130                 135                 140                 


Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro 
145                 150                 155                 160 


Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu 
                165                 170                 175     


Val Lys Leu Thr Gln Gly Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser 
            180                 185                 190         


Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu Gly Leu Ala Arg Val Leu 
        195                 200                 205             


Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala Gly Ala Gln Val Cys Ser 
    210                 215                 220                 


Leu Leu Leu Ala Gln Ser Glu Val Arg Pro Gln Cys Leu Leu Leu Val 
225                 230                 235                 240 


Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys Leu Gln Leu Met Lys Lys 
                245                 250                 255     


His Gln Ser Asp Leu Lys Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln 
            260                 265                 270         


Asp Val Ala Ser His Gln Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu 
        275                 280                 285             


Val Thr Ser Gly Ala Leu Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe 
    290                 295                 300                 


Leu Met Asn Arg Arg Ser Trp Ser Pro 
305                 310             


<210>  19
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A-hCD34 (including P2A peptide)

<400>  19

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro Met Pro Arg Gly Trp Thr Ala Leu Cys Leu 
            20                  25                  30          


Leu Ser Leu Leu Pro Ser Gly Phe Met Ser Leu Asp Asn Asn Gly Thr 
        35                  40                  45              


Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr Phe Ser Asn Val Ser Thr 
    50                  55                  60                  


Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr 
65                  70                  75                  80  


Ser Leu His Pro Val Ser Gln His Gly Asn Glu Ala Thr Thr Asn Ile 
                85                  90                  95      


Thr Glu Thr Thr Val Lys Phe Thr Ser Thr Ser Val Ile Thr Ser Val 
            100                 105                 110         


Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser Gln Thr Ser Val Ile Ser 
        115                 120                 125             


Thr Val Phe Thr Thr Pro Ala Asn Val Ser Thr Pro Glu Thr Thr Leu 
    130                 135                 140                 


Lys Pro Ser Leu Ser Pro Gly Asn Val Ser Asp Leu Ser Thr Thr Ser 
145                 150                 155                 160 


Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro 
                165                 170                 175     


Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu 
            180                 185                 190         


Val Lys Leu Thr Gln Gly Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser 
        195                 200                 205             


Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu Gly Leu Ala Arg Val Leu 
    210                 215                 220                 


Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala Gly Ala Gln Val Cys Ser 
225                 230                 235                 240 


Leu Leu Leu Ala Gln Ser Glu Val Arg Pro Gln Cys Leu Leu Leu Val 
                245                 250                 255     


Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys Leu Gln Leu Met Lys Lys 
            260                 265                 270         


His Gln Ser Asp Leu Lys Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln 
        275                 280                 285             


Asp Val Ala Ser His Gln Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu 
    290                 295                 300                 


Val Thr Ser Gly Ala Leu Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe 
305                 310                 315                 320 


Leu Met Asn Arg Arg Ser Trp Ser Pro Ile 
                325                 330 


<210>  20
<211>  335
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A-TrhCD34 (including P2A peptide)

<400>  20

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro Val Gly Pro Phe Glu Ala Met Pro Arg Gly 
            20                  25                  30          


Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser Gly Phe Met Ser 
        35                  40                  45              


Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr 
    50                  55                  60                  


Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro 
65                  70                  75                  80  


Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly Asn 
                85                  90                  95      


Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser Thr 
            100                 105                 110         


Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser 
        115                 120                 125             


Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val Ser 
    130                 135                 140                 


Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val Ser 
145                 150                 155                 160 


Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro 
                165                 170                 175     


Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys 
            180                 185                 190         


Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu Glu 
        195                 200                 205             


Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu 
    210                 215                 220                 


Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala 
225                 230                 235                 240 


Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg Pro 
                245                 250                 255     


Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys 
            260                 265                 270         


Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly Ile 
        275                 280                 285             


Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser Gln 
    290                 295                 300                 


Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu Leu Ala Val Leu 
305                 310                 315                 320 


Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser Trp Ser Pro 
                325                 330                 335 


<210>  21
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human-Herpes Simplex Virus-1 (HSV) - thymidine kinase (TK)

<400>  21

Met Pro Arg Gly Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser 
1               5                   10                  15      


Gly Phe Met Ser Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro 
            20                  25                  30          


Thr Gln Gly Thr Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu 
        35                  40                  45              


Thr Thr Thr Pro Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser 
    50                  55                  60                  


Gln His Gly Asn Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys 
65                  70                  75                  80  


Phe Thr Ser Thr Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser 
                85                  90                  95      


Ser Val Gln Ser Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro 
            100                 105                 110         


Ala Asn Val Ser Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro 
        115                 120                 125             


Gly Asn Val Ser Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser 
    130                 135                 140                 


Pro Thr Lys Pro Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys 
145                 150                 155                 160 


Ala Glu Ile Lys Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly 
                165                 170                 175     


Ile Cys Leu Glu Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys 
            180                 185                 190         


Asp Arg Gly Glu Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala 
        195                 200                 205             


Asp Ala Asp Ala Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser 
    210                 215                 220                 


Glu Val Arg Pro Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu 
225                 230                 235                 240 


Ile Ser Ser Lys Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys 
                245                 250                 255     


Lys Leu Gly Ile Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln 
            260                 265                 270         


Ser Tyr Ser Gln Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu 
        275                 280                 285             


Leu Ala Val Leu Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser 
    290                 295                 300                 


Trp Ser Pro Thr Gly Glu Gly Gly Gly Gly Gly Asp Leu Gly Gly Val 
305                 310                 315                 320 


Lys Leu Pro His Leu Phe Gly Lys Arg Leu Val Glu Ala Arg Met Ala 
                325                 330                 335     


Ser Tyr Pro Cys His Gln His Ala Ser Ala Phe Asp Gln Ala Ala Arg 
            340                 345                 350         


Ser Arg Gly His Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg Arg Gln 
        355                 360                 365             


Gln Glu Ala Thr Glu Val Arg Leu Glu Gln Lys Met Pro Thr Leu Leu 
    370                 375                 380                 


Arg Val Tyr Ile Asp Gly Pro His Gly Met Gly Lys Thr Thr Thr Thr 
385                 390                 395                 400 


Gln Leu Leu Val Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr Val Pro 
                405                 410                 415     


Glu Pro Met Thr Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr Ile Ala 
            420                 425                 430         


Asn Ile Tyr Thr Thr Gln His Arg Leu Asp Gln Gly Glu Ile Ser Ala 
        435                 440                 445             


Gly Asp Ala Ala Val Val Met Thr Ser Ala Gln Ile Thr Met Gly Met 
    450                 455                 460                 


Pro Tyr Ala Val Thr Asp Ala Val Leu Ala Pro His Val Gly Gly Glu 
465                 470                 475                 480 


Ala Gly Ser Ser His Ala Pro Pro Pro Ala Leu Thr Leu Leu Leu Asp 
                485                 490                 495     


Arg His Pro Ile Ala Val Met Leu Cys Tyr Pro Ala Ala Arg Tyr Leu 
            500                 505                 510         


Met Gly Ser Met Thr Pro Gln Ala Val Leu Ala Phe Val Ala Leu Ile 
        515                 520                 525             


Pro Pro Thr Leu Pro Gly Thr Asn Ile Val Leu Gly Ala Leu Pro Glu 
    530                 535                 540                 


Asp Arg His Ile Asp Arg Leu Ala Lys Arg Gln Arg Pro Gly Glu Arg 
545                 550                 555                 560 


Leu Asp Leu Ala Met Leu Ala Ala Ile Arg Arg Val Tyr Gly Leu Leu 
                565                 570                 575     


Ala Asn Thr Val Arg Tyr Leu Gln Gly Gly Gly Ser Trp Trp Glu Asp 
            580                 585                 590         


Trp Gly Gln Leu Ser Gly Thr Ala Val Pro Pro Gln Gly Ala Glu Pro 
        595                 600                 605             


Gln Ser Asn Ala Gly Pro Arg Pro His Ile Gly Asp Thr Leu Phe Thr 
    610                 615                 620                 


Leu Phe Arg Ala Pro Glu Leu Leu Ala Pro Asn Gly Asp Leu Tyr Asn 
625                 630                 635                 640 


Val Phe Ala Trp Ala Leu Asp Val Leu Ala Lys Arg Leu Arg Pro Met 
                645                 650                 655     


His Val Phe Ile Leu Asp Tyr Asp Gln Ser Pro Ala Gly Cys Arg Asp 
            660                 665                 670         


Ala Leu Leu Gln Leu Thr Ser Gly Met Val Gln Thr His Val Thr Thr 
        675                 680                 685             


Pro Gly Ser Ile Pro Thr Ile Cys Asp Leu Ala Arg Thr Phe Ala Arg 
    690                 695                 700                 


Glu Met Gly Glu Ala Asn 
705                 710 


<210>  22
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7 heavy chain variable region

<400>  22

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly 
1               5                   10                  15      


Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val 
        35                  40                  45              


Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr Pro Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg Asn Ile Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Met Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp Gly Ala Gly Thr Thr 
            100                 105                 110         


Val Thr Val Ser 
        115     


<210>  23
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7 light chain variable region

<400>  23

Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln Gly Ile Ser Asn Tyr 
            20                  25                  30          


Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr Val Lys Leu Leu Ile 
        35                  40                  45              


Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile Ser Asn Leu Glu Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr Ser Lys Leu Pro Tyr 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg 
            100                 105             


<210>  24
<211>  495
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB

<400>  24

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 495 


<210>  25
<211>  494
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-CD28

<400>  25

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val 
    370                 375                 380                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn 
385                 390                 395                 400 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                405                 410                 415     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            420                 425                 430         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        435                 440                 445             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    450                 455                 460                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490                 


<210>  26
<211>  832
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB_CD34

<400>  26

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg 
                485                 490                 495     


Thr Asp Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
            500                 505                 510         


Asp Val Glu Glu Asn Pro Gly Pro Val Ser Glu Ala Met Pro Arg Gly 
        515                 520                 525             


Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser Gly Phe Met Ser 
    530                 535                 540                 


Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr 
545                 550                 555                 560 


Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro 
                565                 570                 575     


Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly Asn 
            580                 585                 590         


Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser Thr 
        595                 600                 605             


Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser 
    610                 615                 620                 


Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val Ser 
625                 630                 635                 640 


Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val Ser 
                645                 650                 655     


Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro 
            660                 665                 670         


Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys 
        675                 680                 685             


Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu Glu 
    690                 695                 700                 


Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu 
705                 710                 715                 720 


Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala 
                725                 730                 735     


Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg Pro 
            740                 745                 750         


Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys 
        755                 760                 765             


Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly Ile 
    770                 775                 780                 


Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser Gln 
785                 790                 795                 800 


Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu Leu Ala Val Leu 
                805                 810                 815     


Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser Trp Ser Pro Ile 
            820                 825                 830         


<210>  27
<211>  1234
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB_CD34_TK

<400>  27

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
            340                 345                 350         


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
        355                 360                 365             


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg 
    370                 375                 380                 


Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln 
385                 390                 395                 400 


Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp 
                405                 410                 415     


Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro 
            420                 425                 430         


Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg 
                485                 490                 495     


Thr Asp Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly 
            500                 505                 510         


Asp Val Glu Glu Asn Pro Gly Pro Val Ser Glu Ala Met Pro Arg Gly 
        515                 520                 525             


Trp Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser Gly Phe Met Ser 
    530                 535                 540                 


Leu Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr 
545                 550                 555                 560 


Phe Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro 
                565                 570                 575     


Ser Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly Asn 
            580                 585                 590         


Glu Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser Thr 
        595                 600                 605             


Ser Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser 
    610                 615                 620                 


Gln Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val Ser 
625                 630                 635                 640 


Thr Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val Ser 
                645                 650                 655     


Asp Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro 
            660                 665                 670         


Tyr Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys 
        675                 680                 685             


Cys Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu Glu 
    690                 695                 700                 


Gln Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu 
705                 710                 715                 720 


Gly Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala 
                725                 730                 735     


Gly Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg Pro 
            740                 745                 750         


Gln Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys 
        755                 760                 765             


Leu Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly Ile 
    770                 775                 780                 


Leu Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser Gln 
785                 790                 795                 800 


Lys Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu Leu Ala Val Leu 
                805                 810                 815     


Gly Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser Trp Ser Pro Thr 
            820                 825                 830         


Gly Glu Gly Gly Gly Gly Gly Asp Leu Gly Gly Val Lys Leu Pro His 
        835                 840                 845             


Leu Phe Gly Lys Arg Leu Val Glu Ala Arg Met Ala Ser Tyr Pro Cys 
    850                 855                 860                 


His Gln His Ala Ser Ala Phe Asp Gln Ala Ala Arg Ser Arg Gly His 
865                 870                 875                 880 


Ser Asn Arg Arg Thr Ala Leu Arg Pro Arg Arg Gln Gln Glu Ala Thr 
                885                 890                 895     


Glu Val Arg Leu Glu Gln Lys Met Pro Thr Leu Leu Arg Val Tyr Ile 
            900                 905                 910         


Asp Gly Pro His Gly Met Gly Lys Thr Thr Thr Thr Gln Leu Leu Val 
        915                 920                 925             


Ala Leu Gly Ser Arg Asp Asp Ile Val Tyr Val Pro Glu Pro Met Thr 
    930                 935                 940                 


Tyr Trp Gln Val Leu Gly Ala Ser Glu Thr Ile Ala Asn Ile Tyr Thr 
945                 950                 955                 960 


Thr Gln His Arg Leu Asp Gln Gly Glu Ile Ser Ala Gly Asp Ala Ala 
                965                 970                 975     


Val Val Met Thr Ser Ala Gln Ile Thr Met Gly Met Pro Tyr Ala Val 
            980                 985                 990         


Thr Asp Ala Val Leu Ala Pro His  Val Gly Gly Glu Ala  Gly Ser Ser 
        995                 1000                 1005             


His Ala  Pro Pro Pro Ala Leu  Thr Leu Leu Leu Asp  Arg His Pro 
    1010                 1015                 1020             


Ile Ala  Val Met Leu Cys Tyr  Pro Ala Ala Arg Tyr  Leu Met Gly 
    1025                 1030                 1035             


Ser Met  Thr Pro Gln Ala Val  Leu Ala Phe Val Ala  Leu Ile Pro 
    1040                 1045                 1050             


Pro Thr  Leu Pro Gly Thr Asn  Ile Val Leu Gly Ala  Leu Pro Glu 
    1055                 1060                 1065             


Asp Arg  His Ile Asp Arg Leu  Ala Lys Arg Gln Arg  Pro Gly Glu 
    1070                 1075                 1080             


Arg Leu  Asp Leu Ala Met Leu  Ala Ala Ile Arg Arg  Val Tyr Gly 
    1085                 1090                 1095             


Leu Leu  Ala Asn Thr Val Arg  Tyr Leu Gln Gly Gly  Gly Ser Trp 
    1100                 1105                 1110             


Trp Glu  Asp Trp Gly Gln Leu  Ser Gly Thr Ala Val  Pro Pro Gln 
    1115                 1120                 1125             


Gly Ala  Glu Pro Gln Ser Asn  Ala Gly Pro Arg Pro  His Ile Gly 
    1130                 1135                 1140             


Asp Thr  Leu Phe Thr Leu Phe  Arg Ala Pro Glu Leu  Leu Ala Pro 
    1145                 1150                 1155             


Asn Gly  Asp Leu Tyr Asn Val  Phe Ala Trp Ala Leu  Asp Val Leu 
    1160                 1165                 1170             


Ala Lys  Arg Leu Arg Pro Met  His Val Phe Ile Leu  Asp Tyr Asp 
    1175                 1180                 1185             


Gln Ser  Pro Ala Gly Cys Arg  Asp Ala Leu Leu Gln  Leu Thr Ser 
    1190                 1195                 1200             


Gly Met  Val Gln Thr His Val  Thr Thr Pro Gly Ser  Ile Pro Thr 
    1205                 1210                 1215             


Ile Cys  Asp Leu Ala Arg Thr  Phe Ala Arg Glu Met  Gly Glu Ala 
    1220                 1225                 1230             


Asn 
    


<210>  28
<211>  831
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-CD28_CD34

<400>  28

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val 
    370                 375                 380                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn 
385                 390                 395                 400 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                405                 410                 415     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            420                 425                 430         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        435                 440                 445             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    450                 455                 460                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Thr 
                485                 490                 495     


Asp Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
            500                 505                 510         


Val Glu Glu Asn Pro Gly Pro Val Ser Glu Ala Met Pro Arg Gly Trp 
        515                 520                 525             


Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser Gly Phe Met Ser Leu 
    530                 535                 540                 


Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr Phe 
545                 550                 555                 560 


Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro Ser 
                565                 570                 575     


Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly Asn Glu 
            580                 585                 590         


Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser Thr Ser 
        595                 600                 605             


Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser Gln 
    610                 615                 620                 


Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val Ser Thr 
625                 630                 635                 640 


Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val Ser Asp 
                645                 650                 655     


Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro Tyr 
            660                 665                 670         


Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys Cys 
        675                 680                 685             


Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu Glu Gln 
    690                 695                 700                 


Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu Gly 
705                 710                 715                 720 


Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala Gly 
                725                 730                 735     


Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg Pro Gln 
            740                 745                 750         


Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys Leu 
        755                 760                 765             


Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly Ile Leu 
    770                 775                 780                 


Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser Gln Lys 
785                 790                 795                 800 


Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu Leu Ala Val Leu Gly 
                805                 810                 815     


Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser Trp Ser Pro Ile 
            820                 825                 830     


<210>  29
<211>  1233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-CD28_CD34_TK

<400>  29

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Pro Arg Ala Ser Thr Thr Thr 
            260                 265                 270         


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
        275                 280                 285             


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
    290                 295                 300                 


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val 
305                 310                 315                 320 


Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe 
                325                 330                 335     


Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser Arg Val 
    370                 375                 380                 


Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn 
385                 390                 395                 400 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                405                 410                 415     


Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg 
            420                 425                 430         


Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys 
        435                 440                 445             


Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg 
    450                 455                 460                 


Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys 
465                 470                 475                 480 


Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Arg Thr 
                485                 490                 495     


Asp Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp 
            500                 505                 510         


Val Glu Glu Asn Pro Gly Pro Val Ser Glu Ala Met Pro Arg Gly Trp 
        515                 520                 525             


Thr Ala Leu Cys Leu Leu Ser Leu Leu Pro Ser Gly Phe Met Ser Leu 
    530                 535                 540                 


Asp Asn Asn Gly Thr Ala Thr Pro Glu Leu Pro Thr Gln Gly Thr Phe 
545                 550                 555                 560 


Ser Asn Val Ser Thr Asn Val Ser Tyr Gln Glu Thr Thr Thr Pro Ser 
                565                 570                 575     


Thr Leu Gly Ser Thr Ser Leu His Pro Val Ser Gln His Gly Asn Glu 
            580                 585                 590         


Ala Thr Thr Asn Ile Thr Glu Thr Thr Val Lys Phe Thr Ser Thr Ser 
        595                 600                 605             


Val Ile Thr Ser Val Tyr Gly Asn Thr Asn Ser Ser Val Gln Ser Gln 
    610                 615                 620                 


Thr Ser Val Ile Ser Thr Val Phe Thr Thr Pro Ala Asn Val Ser Thr 
625                 630                 635                 640 


Pro Glu Thr Thr Leu Lys Pro Ser Leu Ser Pro Gly Asn Val Ser Asp 
                645                 650                 655     


Leu Ser Thr Thr Ser Thr Ser Leu Ala Thr Ser Pro Thr Lys Pro Tyr 
            660                 665                 670         


Thr Ser Ser Ser Pro Ile Leu Ser Asp Ile Lys Ala Glu Ile Lys Cys 
        675                 680                 685             


Ser Gly Ile Arg Glu Val Lys Leu Thr Gln Gly Ile Cys Leu Glu Gln 
    690                 695                 700                 


Asn Lys Thr Ser Ser Cys Ala Glu Phe Lys Lys Asp Arg Gly Glu Gly 
705                 710                 715                 720 


Leu Ala Arg Val Leu Cys Gly Glu Glu Gln Ala Asp Ala Asp Ala Gly 
                725                 730                 735     


Ala Gln Val Cys Ser Leu Leu Leu Ala Gln Ser Glu Val Arg Pro Gln 
            740                 745                 750         


Cys Leu Leu Leu Val Leu Ala Asn Arg Thr Glu Ile Ser Ser Lys Leu 
        755                 760                 765             


Gln Leu Met Lys Lys His Gln Ser Asp Leu Lys Lys Leu Gly Ile Leu 
    770                 775                 780                 


Asp Phe Thr Glu Gln Asp Val Ala Ser His Gln Ser Tyr Ser Gln Lys 
785                 790                 795                 800 


Thr Leu Ile Ala Leu Val Thr Ser Gly Ala Leu Leu Ala Val Leu Gly 
                805                 810                 815     


Ile Thr Gly Tyr Phe Leu Met Asn Arg Arg Ser Trp Ser Pro Thr Gly 
            820                 825                 830         


Glu Gly Gly Gly Gly Gly Asp Leu Gly Gly Val Lys Leu Pro His Leu 
        835                 840                 845             


Phe Gly Lys Arg Leu Val Glu Ala Arg Met Ala Ser Tyr Pro Cys His 
    850                 855                 860                 


Gln His Ala Ser Ala Phe Asp Gln Ala Ala Arg Ser Arg Gly His Ser 
865                 870                 875                 880 


Asn Arg Arg Thr Ala Leu Arg Pro Arg Arg Gln Gln Glu Ala Thr Glu 
                885                 890                 895     


Val Arg Leu Glu Gln Lys Met Pro Thr Leu Leu Arg Val Tyr Ile Asp 
            900                 905                 910         


Gly Pro His Gly Met Gly Lys Thr Thr Thr Thr Gln Leu Leu Val Ala 
        915                 920                 925             


Leu Gly Ser Arg Asp Asp Ile Val Tyr Val Pro Glu Pro Met Thr Tyr 
    930                 935                 940                 


Trp Gln Val Leu Gly Ala Ser Glu Thr Ile Ala Asn Ile Tyr Thr Thr 
945                 950                 955                 960 


Gln His Arg Leu Asp Gln Gly Glu Ile Ser Ala Gly Asp Ala Ala Val 
                965                 970                 975     


Val Met Thr Ser Ala Gln Ile Thr Met Gly Met Pro Tyr Ala Val Thr 
            980                 985                 990         


Asp Ala Val Leu Ala Pro His Val  Gly Gly Glu Ala Gly  Ser Ser His 
        995                 1000                 1005             


Ala Pro  Pro Pro Ala Leu Thr  Leu Leu Leu Asp Arg  His Pro Ile 
    1010                 1015                 1020             


Ala Val  Met Leu Cys Tyr Pro  Ala Ala Arg Tyr Leu  Met Gly Ser 
    1025                 1030                 1035             


Met Thr  Pro Gln Ala Val Leu  Ala Phe Val Ala Leu  Ile Pro Pro 
    1040                 1045                 1050             


Thr Leu  Pro Gly Thr Asn Ile  Val Leu Gly Ala Leu  Pro Glu Asp 
    1055                 1060                 1065             


Arg His  Ile Asp Arg Leu Ala  Lys Arg Gln Arg Pro  Gly Glu Arg 
    1070                 1075                 1080             


Leu Asp  Leu Ala Met Leu Ala  Ala Ile Arg Arg Val  Tyr Gly Leu 
    1085                 1090                 1095             


Leu Ala  Asn Thr Val Arg Tyr  Leu Gln Gly Gly Gly  Ser Trp Trp 
    1100                 1105                 1110             


Glu Asp  Trp Gly Gln Leu Ser  Gly Thr Ala Val Pro  Pro Gln Gly 
    1115                 1120                 1125             


Ala Glu  Pro Gln Ser Asn Ala  Gly Pro Arg Pro His  Ile Gly Asp 
    1130                 1135                 1140             


Thr Leu  Phe Thr Leu Phe Arg  Ala Pro Glu Leu Leu  Ala Pro Asn 
    1145                 1150                 1155             


Gly Asp  Leu Tyr Asn Val Phe  Ala Trp Ala Leu Asp  Val Leu Ala 
    1160                 1165                 1170             


Lys Arg  Leu Arg Pro Met His  Val Phe Ile Leu Asp  Tyr Asp Gln 
    1175                 1180                 1185             


Ser Pro  Ala Gly Cys Arg Asp  Ala Leu Leu Gln Leu  Thr Ser Gly 
    1190                 1195                 1200             


Met Val  Gln Thr His Val Thr  Thr Pro Gly Ser Ile  Pro Thr Ile 
    1205                 1210                 1215             


Cys Asp  Leu Ala Arg Thr Phe  Ala Arg Glu Met Gly  Glu Ala Asn 
    1220                 1225                 1230             


<210>  30
<211>  491
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB (PRAS cloning artifact removed)

<400>  30

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Ser Ala Ser Gln 
        35                  40                  45              


Gly Ile Ser Asn Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr Tyr Thr Ser Ser Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Pro Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Tyr 
            100                 105                 110         


Ser Lys Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
        115                 120                 125             


Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu 
145                 150                 155                 160 


Val Lys Pro Gly Gly Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Leu 
                165                 170                 175     


Thr Phe Ser Ser Tyr Ala Met Ser Trp Val Arg Gln Thr Pro Glu Lys 
            180                 185                 190         


Arg Leu Glu Trp Val Ala Ser Ile Ser Ser Gly Gly Phe Thr Tyr Tyr 
        195                 200                 205             


Pro Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Arg 
    210                 215                 220                 


Asn Ile Leu Tyr Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala 
225                 230                 235                 240 


Met Tyr Tyr Cys Ala Arg Asp Glu Val Arg Gly Tyr Leu Asp Val Trp 
                245                 250                 255     


Gly Ala Gly Thr Thr Val Thr Val Ser Thr Thr Thr Pro Ala Pro Arg 
            260                 265                 270         


Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg 
        275                 280                 285             


Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly 
    290                 295                 300                 


Leu Asp Phe Ala Cys Asp Phe Trp Val Leu Val Val Val Gly Gly Val 
305                 310                 315                 320 


Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe Trp 
                325                 330                 335     


Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe 
            340                 345                 350         


Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg 
        355                 360                 365             


Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser 
    370                 375                 380                 


Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr 
385                 390                 395                 400 


Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys 
                405                 410                 415     


Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn 
            420                 425                 430         


Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu 
        435                 440                 445             


Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly 
    450                 455                 460                 


His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr 
465                 470                 475                 480 


Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
                485                 490     


<210>  31
<211>  1488
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB (complete ORF)

<400>  31
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggatatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagtgcaag tcagggcatt agcaattatt taaactggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctat tacacatcaa gtttacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctgggaca gattattctc tcaccatcag caacctggaa      300

cctgaagata ttgccactta ttattgtcag cagtatagca agcttccgta cacgttcgga      360

ggggggacca agctggaaat aaaacgtggt ggtggtggtt ctggtggtgg tggttctggc      420

ggcggcggct ccggtggtgg tggatccgag gtgcaactgg tggagtctgg gggaggctta      480

gtgaagcctg gggggtccct gaaactctcc tgtgcagcct ctggactcac tttcagtagc      540

tatgccatgt cttgggttcg ccagactcca gagaagaggc tggagtgggt cgcatccatt      600

agtagtggtg gtttcaccta ctatccagac agtgtgaagg gccgattcac catctccaga      660

gataatgcca ggaacatcct gtatctgcaa atgagcagtc tgaggtctga ggacacggcc      720

atgtattact gtgcaagaga cgaggtacgg gggtacctcg atgtctgggg cgcagggacc      780

acggtcaccg tttcccctag ggctagcacc acgacgccag cgccgcgacc accaacaccg      840

gcgcccacca tcgcgtcgca gcccctgtcc ctgcgcccag aggcgtgccg gccagcggcg      900

gggggcgcag tgcacacgag ggggctggac ttcgcctgtg atttttgggt gctggtggtg      960

gttggtggag tcctggcttg ctatagcttg ctagtaacag tggcctttat tattttctgg     1020

gtgaaacggg gcagaaagaa actcctgtat atattcaaac aaccatttat gagaccagta     1080

caaactactc aagaggaaga tggctgtagc tgccgatttc cagaagaaga agaaggagga     1140

tgtgagctaa gagtgaagtt cagcaggagc gcagacgccc ccgcgtacaa gcagggccag     1200

aaccagctct ataacgagct caatctagga cgaagagagg agtacgatgt tttggacaag     1260

agacgtggcc gggaccctga gatgggggga aagccgagaa ggaagaaccc tcaggaaggc     1320

ctgtacaatg aactgcagaa agataagatg gcggaggcct acagtgagat tgggatgaaa     1380

ggcgagcgcc ggaggggcaa ggggcacgat ggcctttacc agggtctcag tacagccacc     1440

aaggacacct acgacgccct tcacatgcag gccctgcccc ctcgctaa                  1488


<210>  32
<211>  1476
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD7-CAR-4-1BB (PRAS cloning artifact removed, complete ORF)

<400>  32
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggatatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagtgcaag tcagggcatt agcaattatt taaactggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctat tacacatcaa gtttacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctgggaca gattattctc tcaccatcag caacctggaa      300

cctgaagata ttgccactta ttattgtcag cagtatagca agcttccgta cacgttcgga      360

ggggggacca agctggaaat aaaacgtggt ggtggtggtt ctggtggtgg tggttctggc      420

ggcggcggct ccggtggtgg tggatccgag gtgcaactgg tggagtctgg gggaggctta      480

gtgaagcctg gggggtccct gaaactctcc tgtgcagcct ctggactcac tttcagtagc      540

tatgccatgt cttgggttcg ccagactcca gagaagaggc tggagtgggt cgcatccatt      600

agtagtggtg gtttcaccta ctatccagac agtgtgaagg gccgattcac catctccaga      660

gataatgcca ggaacatcct gtatctgcaa atgagcagtc tgaggtctga ggacacggcc      720

atgtattact gtgcaagaga cgaggtacgg gggtacctcg atgtctgggg cgcagggacc      780

acggtcaccg tttccaccac gacgccagcg ccgcgaccac caacaccggc gcccaccatc      840

gcgtcgcagc ccctgtccct gcgcccagag gcgtgccggc cagcggcggg gggcgcagtg      900

cacacgaggg ggctggactt cgcctgtgat ttttgggtgc tggtggtggt tggtggagtc      960

ctggcttgct atagcttgct agtaacagtg gcctttatta ttttctgggt gaaacggggc     1020

agaaagaaac tcctgtatat attcaaacaa ccatttatga gaccagtaca aactactcaa     1080

gaggaagatg gctgtagctg ccgatttcca gaagaagaag aaggaggatg tgagctaaga     1140

gtgaagttca gcaggagcgc agacgccccc gcgtacaagc agggccagaa ccagctctat     1200

aacgagctca atctaggacg aagagaggag tacgatgttt tggacaagag acgtggccgg     1260

gaccctgaga tggggggaaa gccgagaagg aagaaccctc aggaaggcct gtacaatgaa     1320

ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg     1380

aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac     1440

gacgcccttc acatgcaggc cctgccccct cgctaa                               1476


