                         SEQUENCE LISTING

<110>  Protalix Ltd.
       RUDERFER, Ilya
       NATAF, Yakir
       ARVATZ, Gil
       HANANIA, Uri
       ARIEL, Tamar
       ROZEN, Shelly
       HAYON, Yael
 
<120>  MODIFIED URICASE AND USES THEREOF

<130>  89419

<150>  US 63/108,890
<151>  2020-11-03

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  303
<212>  PRT
<213>  Candida utilis

<400>  1

Met Ser Thr Thr Leu Ser Ser Ser Thr Tyr Gly Lys Asp Asn Val Lys 
1               5                   10                  15      


Phe Leu Lys Val Lys Lys Asp Pro Gln Asn Pro Lys Lys Gln Glu Val 
            20                  25                  30          


Met Glu Ala Thr Val Thr Cys Leu Leu Glu Gly Gly Phe Asp Thr Ser 
        35                  40                  45              


Tyr Thr Glu Ala Asp Asn Ser Ser Ile Val Pro Thr Asp Thr Val Lys 
    50                  55                  60                  


Asn Thr Ile Leu Val Leu Ala Lys Thr Thr Glu Ile Trp Pro Ile Glu 
65                  70                  75                  80  


Arg Phe Ala Ala Lys Leu Ala Thr His Phe Val Glu Lys Tyr Ser His 
                85                  90                  95      


Val Ser Gly Val Ser Val Lys Ile Val Gln Asp Arg Trp Val Lys Tyr 
            100                 105                 110         


Ala Val Asp Gly Lys Pro His Asp His Ser Phe Ile His Glu Gly Gly 
        115                 120                 125             


Glu Lys Arg Ile Thr Asp Leu Tyr Tyr Lys Arg Ser Gly Asp Tyr Lys 
    130                 135                 140                 


Leu Ser Ser Ala Ile Lys Asp Leu Thr Val Leu Lys Ser Thr Gly Ser 
145                 150                 155                 160 


Met Phe Tyr Gly Tyr Asn Lys Cys Asp Phe Thr Thr Leu Gln Pro Thr 
                165                 170                 175     


Thr Asp Arg Ile Leu Ser Thr Asp Val Asp Ala Thr Trp Val Trp Asp 
            180                 185                 190         


Asn Lys Lys Ile Gly Ser Val Tyr Asp Ile Ala Lys Ala Ala Asp Lys 
        195                 200                 205             


Gly Ile Phe Asp Asn Val Tyr Asn Gln Ala Arg Glu Ile Thr Leu Thr 
    210                 215                 220                 


Thr Phe Ala Leu Glu Asn Ser Pro Ser Val Gln Ala Thr Met Phe Asn 
225                 230                 235                 240 


Met Ala Thr Gln Ile Leu Glu Lys Ala Cys Ser Val Tyr Ser Val Ser 
                245                 250                 255     


Tyr Ala Leu Pro Asn Lys His Tyr Phe Leu Ile Asp Leu Lys Trp Lys 
            260                 265                 270         


Gly Leu Glu Asn Asp Asn Glu Leu Phe Tyr Pro Ser Pro His Pro Asn 
        275                 280                 285             


Gly Leu Ile Lys Cys Thr Val Val Arg Lys Glu Lys Thr Lys Leu 
    290                 295                 300             


<210>  2
<211>  303
<212>  PRT
<213>  Artificial sequence

<220>
<223>  C250K point mutation of SEQ ID NO: 1

<400>  2

Met Ser Thr Thr Leu Ser Ser Ser Thr Tyr Gly Lys Asp Asn Val Lys 
1               5                   10                  15      


Phe Leu Lys Val Lys Lys Asp Pro Gln Asn Pro Lys Lys Gln Glu Val 
            20                  25                  30          


Met Glu Ala Thr Val Thr Cys Leu Leu Glu Gly Gly Phe Asp Thr Ser 
        35                  40                  45              


Tyr Thr Glu Ala Asp Asn Ser Ser Ile Val Pro Thr Asp Thr Val Lys 
    50                  55                  60                  


Asn Thr Ile Leu Val Leu Ala Lys Thr Thr Glu Ile Trp Pro Ile Glu 
65                  70                  75                  80  


Arg Phe Ala Ala Lys Leu Ala Thr His Phe Val Glu Lys Tyr Ser His 
                85                  90                  95      


Val Ser Gly Val Ser Val Lys Ile Val Gln Asp Arg Trp Val Lys Tyr 
            100                 105                 110         


Ala Val Asp Gly Lys Pro His Asp His Ser Phe Ile His Glu Gly Gly 
        115                 120                 125             


Glu Lys Arg Ile Thr Asp Leu Tyr Tyr Lys Arg Ser Gly Asp Tyr Lys 
    130                 135                 140                 


Leu Ser Ser Ala Ile Lys Asp Leu Thr Val Leu Lys Ser Thr Gly Ser 
145                 150                 155                 160 


Met Phe Tyr Gly Tyr Asn Lys Cys Asp Phe Thr Thr Leu Gln Pro Thr 
                165                 170                 175     


Thr Asp Arg Ile Leu Ser Thr Asp Val Asp Ala Thr Trp Val Trp Asp 
            180                 185                 190         


Asn Lys Lys Ile Gly Ser Val Tyr Asp Ile Ala Lys Ala Ala Asp Lys 
        195                 200                 205             


Gly Ile Phe Asp Asn Val Tyr Asn Gln Ala Arg Glu Ile Thr Leu Thr 
    210                 215                 220                 


Thr Phe Ala Leu Glu Asn Ser Pro Ser Val Gln Ala Thr Met Phe Asn 
225                 230                 235                 240 


Met Ala Thr Gln Ile Leu Glu Lys Ala Lys Ser Val Tyr Ser Val Ser 
                245                 250                 255     


Tyr Ala Leu Pro Asn Lys His Tyr Phe Leu Ile Asp Leu Lys Trp Lys 
            260                 265                 270         


Gly Leu Glu Asn Asp Asn Glu Leu Phe Tyr Pro Ser Pro His Pro Asn 
        275                 280                 285             


Gly Leu Ile Lys Cys Thr Val Val Arg Lys Glu Lys Thr Lys Leu 
    290                 295                 300             


<210>  3
<211>  308
<212>  PRT
<213>  Arthrobacter gangotriensis

<400>  3

Met Thr Thr Phe Ala Thr Lys Thr Thr Thr Asp Thr Lys Ile Val Leu 
1               5                   10                  15      


Gly Ser Asn Gln Tyr Gly Lys Ala Glu Val Arg Leu Val Lys Ile Thr 
            20                  25                  30          


Arg Asp Thr Asp Arg His Gln Ile Glu Asp Leu Asn Val Thr Ser Gln 
        35                  40                  45              


Leu His Gly Asp Phe Leu Ala Ala His Ile Asp Gly Asp Asn Ala His 
    50                  55                  60                  


Val Val Ala Thr Asp Thr Gln Lys Asn Thr Val Tyr Gly Leu Ala Lys 
65                  70                  75                  80  


Asp Gly Val Gly Ser Pro Glu Glu Phe Leu Ala Arg Leu Gly Gln His 
                85                  90                  95      


Phe Thr Gly Glu Phe Asp Trp Val Ser Gly Gly Arg Trp Ala Ala Glu 
            100                 105                 110         


Gln Tyr Phe Trp Asp Arg Ile Gln Asp His Asp His Ala Phe Ser Arg 
        115                 120                 125             


Asn Lys Ser Glu Val Arg Thr Ala Val Leu Glu Ile Arg Asn Gly Glu 
    130                 135                 140                 


Thr Ser Val Leu Ala Gly Ile Gln Asp Leu Thr Val Leu Lys Ser Thr 
145                 150                 155                 160 


Ala Ser Glu Phe Arg Gly Phe Pro Arg Asp Lys Tyr Thr Thr Leu Gln 
                165                 170                 175     


Glu Thr Asp Asp Arg Ile Leu Ala Thr Asp Val Thr Ala Arg Trp Arg 
            180                 185                 190         


Tyr Asn Ser Glu Thr Ile Glu Ala Ser Gly Leu Asp Phe Asn Ala Val 
        195                 200                 205             


Tyr Ala Ser Val Arg Glu Leu Leu Leu Ala Gly Phe Ser Ala Thr His 
    210                 215                 220                 


Ser Tyr Ala Leu Gln Gln Thr Met Phe Glu Met Gly Lys Ala Val Leu 
225                 230                 235                 240 


Glu Ala His Pro Glu Ile Glu Glu Ile Lys Phe Ser Leu Pro Asn Lys 
                245                 250                 255     


His His Phe Leu Val Asp Leu Thr Pro Phe Gly Gln Asp Asn Pro Asn 
            260                 265                 270         


Glu Val Phe Phe Ala Ala Asp Arg Pro Tyr Gly Leu Ile Glu Ala Thr 
        275                 280                 285             


Ile Thr Arg Glu Gly Val Pro Ala Asn His Pro Ile Trp Glu Asn Thr 
    290                 295                 300                 


Pro Gly Phe Cys 
305             


<210>  4
<211>  302
<212>  PRT
<213>  Aspergillus flavus

<400>  4

Met Ser Ala Val Lys Ala Ala Arg Tyr Gly Lys Asp Asn Val Arg Val 
1               5                   10                  15      


Tyr Lys Val His Lys Asp Glu Lys Thr Gly Val Gln Thr Val Tyr Glu 
            20                  25                  30          


Met Thr Val Cys Val Leu Leu Glu Gly Glu Ile Glu Thr Ser Tyr Thr 
        35                  40                  45              


Lys Ala Asp Asn Ser Val Ile Val Ala Thr Asp Ser Ile Lys Asn Thr 
    50                  55                  60                  


Ile Tyr Ile Thr Ala Lys Gln Asn Pro Val Thr Pro Pro Glu Leu Phe 
65                  70                  75                  80  


Gly Ser Ile Leu Gly Thr His Phe Ile Glu Lys Tyr Asn His Ile His 
                85                  90                  95      


Ala Ala His Val Asn Ile Val Cys His Arg Trp Thr Arg Met Asp Ile 
            100                 105                 110         


Asp Gly Lys Pro His Pro His Ser Phe Ile Arg Asp Ser Glu Glu Lys 
        115                 120                 125             


Arg Asn Val Gln Val Asp Val Val Glu Gly Lys Gly Ile Asp Ile Lys 
    130                 135                 140                 


Ser Ser Leu Ser Gly Leu Thr Val Leu Lys Ser Thr Asn Ser Gln Phe 
145                 150                 155                 160 


Trp Gly Phe Leu Arg Asp Glu Tyr Thr Thr Leu Lys Glu Thr Trp Asp 
                165                 170                 175     


Arg Ile Leu Ser Thr Asp Val Asp Ala Thr Trp Gln Trp Lys Asn Phe 
            180                 185                 190         


Ser Gly Leu Gln Glu Val Arg Ser His Val Pro Lys Phe Asp Ala Thr 
        195                 200                 205             


Trp Ala Thr Ala Arg Glu Val Thr Leu Lys Thr Phe Ala Glu Asp Asn 
    210                 215                 220                 


Ser Ala Ser Val Gln Ala Thr Met Tyr Lys Met Ala Glu Gln Ile Leu 
225                 230                 235                 240 


Ala Arg Gln Gln Leu Ile Glu Thr Val Glu Tyr Ser Leu Pro Asn Lys 
                245                 250                 255     


His Tyr Phe Glu Ile Asp Leu Ser Trp His Lys Gly Leu Gln Asn Thr 
            260                 265                 270         


Gly Lys Asn Ala Glu Val Phe Ala Pro Gln Ser Asp Pro Asn Gly Leu 
        275                 280                 285             


Ile Lys Cys Thr Val Gly Arg Ser Ser Leu Lys Ser Lys Leu 
    290                 295                 300         


