                         SEQUENCE LISTING

<110>  QUELL THERAPEUTICS LIMITED
 
<120>  METHOD FOR CRYOPRESERVING ENGINEERED TREGS

<130>  27.130.151796/03

<150>  GB 2017678.0
<151>  2020-11-09

<150>  GB 2107418.2
<151>  2021-05-25

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  431
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met 
                245                 250                 255     


Gln Ala His Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val 
            260                 265                 270         


Ala Ser Ser Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln 
        275                 280                 285             


Gly Pro Val Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser 
    290                 295                 300                 


Leu Phe Ala Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr 
305                 310                 315                 320 


Phe Pro Glu Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met 
                325                 330                 335     


Arg Pro Pro Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu 
            340                 345                 350         


Ala Pro Glu Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr 
        355                 360                 365             


Arg Met Phe Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala 
    370                 375                 380                 


Ile Arg His Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser 
385                 390                 395                 400 


Glu Lys Gly Ala Val Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys 
                405                 410                 415     


Arg Ser Gln Arg Pro Ser Arg Cys Ser Asn Pro Thr Pro Gly Pro 
            420                 425                 430     


<210>  2
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOXP3, aa418 mutant

<400>  2

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met 
                245                 250                 255     


Gln Ala His Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val 
            260                 265                 270         


Ala Ser Ser Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln 
        275                 280                 285             


Gly Pro Val Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser 
    290                 295                 300                 


Leu Phe Ala Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr 
305                 310                 315                 320 


Phe Pro Glu Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met 
                325                 330                 335     


Arg Pro Pro Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu 
            340                 345                 350         


Ala Pro Glu Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr 
        355                 360                 365             


Arg Met Phe Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala 
    370                 375                 380                 


Ile Arg His Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser 
385                 390                 395                 400 


Glu Lys Gly Ala Val Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys 
                405                 410                 415     


Arg Glu Gln Arg Pro Ser Arg Cys Ser Asn Pro Thr Pro Gly Pro 
            420                 425                 430     


<210>  3
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOXP3, aa422 mutant

<400>  3

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met 
                245                 250                 255     


Gln Ala His Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val 
            260                 265                 270         


Ala Ser Ser Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln 
        275                 280                 285             


Gly Pro Val Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser 
    290                 295                 300                 


Leu Phe Ala Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr 
305                 310                 315                 320 


Phe Pro Glu Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met 
                325                 330                 335     


Arg Pro Pro Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu 
            340                 345                 350         


Ala Pro Glu Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr 
        355                 360                 365             


Arg Met Phe Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala 
    370                 375                 380                 


Ile Arg His Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser 
385                 390                 395                 400 


Glu Lys Gly Ala Val Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys 
                405                 410                 415     


Arg Ser Gln Arg Pro Ala Arg Cys Ser Asn Pro Thr Pro Gly Pro 
            420                 425                 430     


<210>  4
<211>  431
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOXP3, aa 418 and 422 mutant

<400>  4

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met 
                245                 250                 255     


Gln Ala His Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val 
            260                 265                 270         


Ala Ser Ser Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln 
        275                 280                 285             


Gly Pro Val Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser 
    290                 295                 300                 


Leu Phe Ala Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr 
305                 310                 315                 320 


Phe Pro Glu Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met 
                325                 330                 335     


Arg Pro Pro Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu 
            340                 345                 350         


Ala Pro Glu Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr 
        355                 360                 365             


Arg Met Phe Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala 
    370                 375                 380                 


Ile Arg His Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser 
385                 390                 395                 400 


Glu Lys Gly Ala Val Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys 
                405                 410                 415     


Arg Glu Gln Arg Pro Ala Arg Cys Ser Asn Pro Thr Pro Gly Pro 
            420                 425                 430     


<210>  5
<211>  362
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOXP3, truncated variant

<400>  5

Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met Pro Pro Ser 
1               5                   10                  15      


Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala Pro Ser Gly 
            20                  25                  30          


Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu Gln Asp Arg 
        35                  40                  45              


Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His Ala Arg Thr 
    50                  55                  60                  


Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met Ile Ser Leu 
65                  70                  75                  80  


Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys Ala Arg Pro 
                85                  90                  95      


Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp Val Ser Arg 
            100                 105                 110         


Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala Pro Arg Lys 
        115                 120                 125             


Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro Leu Leu Ala 
    130                 135                 140                 


Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe Glu Glu Pro 
145                 150                 155                 160 


Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu Asp Glu Lys 
                165                 170                 175     


Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln Ser Leu Glu 
            180                 185                 190         


Gln Gln Leu Val Leu Glu Lys Glu Lys Leu Ser Ala Met Gln Ala His 
        195                 200                 205             


Leu Ala Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val Ala Ser Ser 
    210                 215                 220                 


Asp Lys Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln Gly Pro Val 
225                 230                 235                 240 


Val Pro Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser Leu Phe Ala 
                245                 250                 255     


Val Arg Arg His Leu Trp Gly Ser His Gly Asn Ser Thr Phe Pro Glu 
            260                 265                 270         


Phe Leu His Asn Met Asp Tyr Phe Lys Phe His Asn Met Arg Pro Pro 
        275                 280                 285             


Phe Thr Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu Ala Pro Glu 
    290                 295                 300                 


Lys Gln Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr Arg Met Phe 
305                 310                 315                 320 


Ala Phe Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala Ile Arg His 
                325                 330                 335     


Asn Leu Ser Leu His Lys Cys Phe Val Arg Val Glu Ser Glu Lys Gly 
            340                 345                 350         


Ala Val Trp Thr Val Asp Glu Leu Glu Phe 
        355                 360         


<210>  6
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FOXP3, illustrative variant

<400>  6

Met Pro Asn Pro Arg Pro Gly Lys Pro Ser Ala Pro Ser Leu Ala Leu 
1               5                   10                  15      


Gly Pro Ser Pro Gly Ala Ser Pro Ser Trp Arg Ala Ala Pro Lys Ala 
            20                  25                  30          


Ser Asp Leu Leu Gly Ala Arg Gly Pro Gly Gly Thr Phe Gln Gly Arg 
        35                  40                  45              


Asp Leu Arg Gly Gly Ala His Ala Ser Ser Ser Ser Leu Asn Pro Met 
    50                  55                  60                  


Pro Pro Ser Gln Leu Gln Leu Pro Thr Leu Pro Leu Val Met Val Ala 
65                  70                  75                  80  


Pro Ser Gly Ala Arg Leu Gly Pro Leu Pro His Leu Gln Ala Leu Leu 
                85                  90                  95      


Gln Asp Arg Pro His Phe Met His Gln Leu Ser Thr Val Asp Ala His 
            100                 105                 110         


Ala Arg Thr Pro Val Leu Gln Val His Pro Leu Glu Ser Pro Ala Met 
        115                 120                 125             


Ile Ser Leu Thr Pro Pro Thr Thr Ala Thr Gly Val Phe Ser Leu Lys 
    130                 135                 140                 


Ala Arg Pro Gly Leu Pro Pro Gly Ile Asn Val Ala Ser Leu Glu Trp 
145                 150                 155                 160 


Val Ser Arg Glu Pro Ala Leu Leu Cys Thr Phe Pro Asn Pro Ser Ala 
                165                 170                 175     


Pro Arg Lys Asp Ser Thr Leu Ser Ala Val Pro Gln Ser Ser Tyr Pro 
            180                 185                 190         


Leu Leu Ala Asn Gly Val Cys Lys Trp Pro Gly Cys Glu Lys Val Phe 
        195                 200                 205             


Glu Glu Pro Glu Asp Phe Leu Lys His Cys Gln Ala Asp His Leu Leu 
    210                 215                 220                 


Asp Glu Lys Gly Arg Ala Gln Cys Leu Leu Gln Arg Glu Met Val Gln 
225                 230                 235                 240 


Ser Leu Glu Gln Val Glu Glu Leu Ser Ala Met Gln Ala His Leu Ala 
                245                 250                 255     


Gly Lys Met Ala Leu Thr Lys Ala Ser Ser Val Ala Ser Ser Asp Lys 
            260                 265                 270         


Gly Ser Cys Cys Ile Val Ala Ala Gly Ser Gln Gly Pro Val Val Pro 
        275                 280                 285             


Ala Trp Ser Gly Pro Arg Glu Ala Pro Asp Ser Leu Phe Ala Val Arg 
    290                 295                 300                 


Arg His Leu Trp Gly Ser His Gly Asn Ser Thr Phe Pro Glu Phe Leu 
305                 310                 315                 320 


His Asn Met Asp Tyr Phe Lys Phe His Asn Met Arg Pro Pro Phe Thr 
                325                 330                 335     


Tyr Ala Thr Leu Ile Arg Trp Ala Ile Leu Glu Ala Pro Glu Lys Gln 
            340                 345                 350         


Arg Thr Leu Asn Glu Ile Tyr His Trp Phe Thr Arg Met Phe Ala Phe 
        355                 360                 365             


Phe Arg Asn His Pro Ala Thr Trp Lys Asn Ala Ile Arg His Asn Leu 
    370                 375                 380                 


Ser Leu His Lys Cys Phe Val Arg Val Glu Ser Glu Lys Gly Ala Val 
385                 390                 395                 400 


Trp Thr Val Asp Glu Leu Glu Phe Arg Lys Lys Arg Ser Gln Arg Pro 
                405                 410                 415     


Ser Arg Cys Ser Asn Pro Thr Pro Gly Pro Glu Gly Arg Gly Ser Leu 
            420                 425                 430         


Leu Thr Cys Gly Asp Val Glu Glu Asn 
        435                 440     


<210>  7
<211>  1296
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FOXP3, Illustrative FOXP3 polynucleotide

<400>  7
atgcccaacc ccaggcctgg caagccctcg gccccttcct tggcccttgg cccatcccca       60

ggagcctcgc ccagctggag ggctgcaccc aaagcctcag acctgctggg ggcccggggc      120

ccagggggaa ccttccaggg ccgagatctt cgaggcgggg cccatgcctc ctcttcttcc      180

ttgaacccca tgccaccatc gcagctgcag ctgcccacac tgcccctagt catggtggca      240

ccctccgggg cacggctggg ccccttgccc cacttacagg cactcctcca ggacaggcca      300

catttcatgc accagctctc aacggtggat gcccacgccc ggacccctgt gctgcaggtg      360

caccccctgg agagcccagc catgatcagc ctcacaccac ccaccaccgc cactggggtc      420

ttctccctca aggcccggcc tggcctccca cctgggatca acgtggccag cctggaatgg      480

gtgtccaggg agccggcact gctctgcacc ttcccaaatc ccagtgcacc caggaaggac      540

agcacccttt cggctgtgcc ccagagctcc tacccactgc tggcaaatgg tgtctgcaag      600

tggcccggat gtgagaaggt cttcgaagag ccagaggact tcctcaagca ctgccaggcg      660

gaccatcttc tggatgagaa gggcagggca caatgtctcc tccagagaga gatggtacag      720

tctctggagc agcagctggt gctggagaag gagaagctga gtgccatgca ggcccacctg      780

gctgggaaaa tggcactgac caaggcttca tctgtggcat catccgacaa gggctcctgc      840

tgcatcgtag ctgctggcag ccaaggccct gtcgtcccag cctggtctgg cccccgggag      900

gcccctgaca gcctgtttgc tgtccggagg cacctgtggg gtagccatgg aaacagcaca      960

ttcccagagt tcctccacaa catggactac ttcaagttcc acaacatgcg accccctttc     1020

acctacgcca cgctcatccg ctgggccatc ctggaggctc cagagaagca gcggacactc     1080

aatgagatct accactggtt cacacgcatg tttgccttct tcagaaacca tcctgccacc     1140

tggaagaacg ccatccgcca caacctgagt ctgcacaagt gctttgtgcg ggtggagagc     1200

gagaaggggg ctgtgtggac cgtggatgag ctggagttcc gcaagaaacg gagccagagg     1260

cccagcaggt gttccaaccc tacacctggc ccctga                               1296


<210>  8
<211>  1352
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FOXP3, Illustrative FOXP3 polynucleotide

<400>  8
gaattcgtcg acatgcccaa ccccagaccc ggcaagcctt ctgccccttc tctggccctg       60

ggaccatctc ctggcgcctc cccatcttgg agagccgccc ctaaagccag cgatctgctg      120

ggagctagag gccctggcgg cacattccag ggcagagatc tgagaggcgg agcccacgcc      180

tctagcagca gcctgaatcc catgccccct agccagctgc agctgcctac actgcctctc      240

gtgatggtgg cccctagcgg agctagactg ggccctctgc ctcatctgca ggctctgctg      300

caggaccggc cccactttat gcaccagctg agcaccgtgg acgcccacgc cagaacacct      360

gtgctgcagg tgcaccccct ggaaagccct gccatgatca gcctgacccc tccaaccaca      420

gccaccggcg tgttcagcct gaaggccaga cctggactgc cccctggcat caatgtggcc      480

agcctggaat gggtgtcccg cgaacctgcc ctgctgtgca ccttccccaa tcctagcgcc      540

cccagaaagg acagcacact gtctgccgtg ccccagagca gctatcccct gctggctaac      600

ggcgtgtgca agtggcctgg ctgcgagaag gtgttcgagg aacccgagga cttcctgaag      660

cactgccagg ccgaccatct gctggacgag aaaggcagag cccagtgcct gctgcagcgc      720

gagatggtgc agtccctgga acagcagctg gtgctggaaa aagaaaagct gagcgccatg      780

caggcccacc tggccggaaa gatggccctg acaaaagcca gcagcgtggc cagctccgac      840

aagggcagct gttgtatcgt ggccgctggc agccagggac ctgtggtgcc tgcttggagc      900

ggacctagag aggcccccga tagcctgttt gccgtgcgga gacacctgtg gggcagccac      960

ggcaactcta ccttccccga gttcctgcac aacatggact acttcaagtt ccacaacatg     1020

aggcccccct tcacctacgc caccctgatc agatgggcca ttctggaagc ccccgagaag     1080

cagcggaccc tgaacgagat ctaccactgg tttacccgga tgttcgcctt cttccggaac     1140

caccccgcca cctggaagaa cgccatccgg cacaatctga gcctgcacaa gtgcttcgtg     1200

cgggtggaaa gcgagaaggg cgccgtgtgg acagtggacg agctggaatt tcggaagaag     1260

cggtcccaga ggcccagccg gtgtagcaat cctacacctg gccctgaggg cagaggaagt     1320

ctgctaacat gcggtgacgt cgaggagaat cc                                   1352


<210>  9
<211>  372
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD62L

<400>  9

Met Ile Phe Pro Trp Lys Cys Gln Ser Thr Gln Arg Asp Leu Trp Asn 
1               5                   10                  15      


Ile Phe Lys Leu Trp Gly Trp Thr Met Leu Cys Cys Asp Phe Leu Ala 
            20                  25                  30          


His His Gly Thr Asp Cys Trp Thr Tyr His Tyr Ser Glu Lys Pro Met 
        35                  40                  45              


Asn Trp Gln Arg Ala Arg Arg Phe Cys Arg Asp Asn Tyr Thr Asp Leu 
    50                  55                  60                  


Val Ala Ile Gln Asn Lys Ala Glu Ile Glu Tyr Leu Glu Lys Thr Leu 
65                  70                  75                  80  


Pro Phe Ser Arg Ser Tyr Tyr Trp Ile Gly Ile Arg Lys Ile Gly Gly 
                85                  90                  95      


Ile Trp Thr Trp Val Gly Thr Asn Lys Ser Leu Thr Glu Glu Ala Glu 
            100                 105                 110         


Asn Trp Gly Asp Gly Glu Pro Asn Asn Lys Lys Asn Lys Glu Asp Cys 
        115                 120                 125             


Val Glu Ile Tyr Ile Lys Arg Asn Lys Asp Ala Gly Lys Trp Asn Asp 
    130                 135                 140                 


Asp Ala Cys His Lys Leu Lys Ala Ala Leu Cys Tyr Thr Ala Ser Cys 
145                 150                 155                 160 


Gln Pro Trp Ser Cys Ser Gly His Gly Glu Cys Val Glu Ile Ile Asn 
                165                 170                 175     


Asn Tyr Thr Cys Asn Cys Asp Val Gly Tyr Tyr Gly Pro Gln Cys Gln 
            180                 185                 190         


Phe Val Ile Gln Cys Glu Pro Leu Glu Ala Pro Glu Leu Gly Thr Met 
        195                 200                 205             


Asp Cys Thr His Pro Leu Gly Asn Phe Ser Phe Ser Ser Gln Cys Ala 
    210                 215                 220                 


Phe Ser Cys Ser Glu Gly Thr Asn Leu Thr Gly Ile Glu Glu Thr Thr 
225                 230                 235                 240 


Cys Gly Pro Phe Gly Asn Trp Ser Ser Pro Glu Pro Thr Cys Gln Val 
                245                 250                 255     


Ile Gln Cys Glu Pro Leu Ser Ala Pro Asp Leu Gly Ile Met Asn Cys 
            260                 265                 270         


Ser His Pro Leu Ala Ser Phe Ser Phe Thr Ser Ala Cys Thr Phe Ile 
        275                 280                 285             


Cys Ser Glu Gly Thr Glu Leu Ile Gly Lys Lys Lys Thr Ile Cys Glu 
    290                 295                 300                 


Ser Ser Gly Ile Trp Ser Asn Pro Ser Pro Ile Cys Gln Lys Leu Asp 
305                 310                 315                 320 


Lys Ser Phe Ser Met Ile Lys Glu Gly Asp Tyr Asn Pro Leu Phe Ile 
                325                 330                 335     


Pro Val Ala Val Met Val Thr Ala Phe Ser Gly Leu Ala Phe Ile Ile 
            340                 345                 350         


Trp Leu Ala Arg Arg Leu Lys Lys Gly Lys Lys Ser Lys Arg Ser Met 
        355                 360                 365             


Asn Asp Pro Tyr 
    370         


<210>  10
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VH CDR1

<400>  10

Asp Tyr Gly Met His 
1               5   


<210>  11
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VH CDR2

<400>  11

Phe Ile Arg Asn Asp Gly Ser Asp Lys Tyr Tyr Ala Asp Ser Val Lys 
1               5                   10                  15      


Gly 
    


<210>  12
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VH CDR3

<400>  12

Asn Gly Glu Ser Gly Pro Leu Asp Tyr Trp Tyr Leu Asp Leu 
1               5                   10                  


<210>  13
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VL CDR1

<400>  13

Gln Ser Ser Leu Asp Ile Ser His Tyr Leu Asn 
1               5                   10      


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VL CDR2

<400>  14

Asp Ala Ser Asn Leu Glu Thr 
1               5           


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  3PB2 VL CDR3

<400>  15

Gln Gln Tyr Asp Asn Leu Pro Leu Thr 
1               5                   


