﻿序列表 

<110>  南京北恒生物科技有限公司
       江苏浦珠生物医药科技有限公司
 
<120>  靶向CD7的人源化抗体及其用途

<130>  BHPCT16

<160>  45  

<170>  PatentIn version 3.5

<210>  1
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-L1

<400>  1
Arg Ala Ser Gln Ser Ile Ser Asn Asn Leu His
                5                   10


<210>  2
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-L2

<400>  2
Ser Ala Ser Gln Ser Ile Ser
                5


<210>  3
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-L3

<400>  3
Gln Gln Ser Asn Ser Trp Pro Tyr Thr
                5                   


<210>  4
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-H1

<400>  4
Gly Tyr Thr Phe Thr Ser Tyr
                5


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-H2

<400>  5
Asn Pro Ser Asn Gly Arg
                5


<210>  6
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CDR-H3

<400>  6
Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr
                5                   10                 


<210>  7
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  m189 

<400>  7
Asp Ile Glu Leu Thr Gln Ser Pro Ala Thr Leu Ser Val Thr Pro Gly
                5                   10                  15

Asp Ser Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Arg Phe Ser Gly
    50                  55                  60

Ser Gly Ser Gly Thr Asp Phe Thr Leu Ser Ile Asn Ser Val Glu Thr
65                  70                  75                  80

Glu Asp Phe Gly Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Gly Ser Thr Ser
            100                 105                 110

Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val
        115                 120                 125

Gln Leu Gln Glu Ser Gly Ala Glu Leu Val Lys Pro Gly Ala Ser Val
    130                 135                 140

Lys Leu Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met
145                 150                 155                 160

His Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly Lys
                165                 170                 175

Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Asn Glu Lys Phe Lys Ser
            180                 185                 190

Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Gln 
        195                 200                 205

Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg
    210                 215                 220

Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr Trp Gly
225                 230                 235                 240

Gln Gly Thr Thr Val Thr Val Ser Ser
                245                                


<210>  8
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-1 VL

<400>  8
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
                5                   10                  15

Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45

Tyr Ser Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60

Ser Gly Pro Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80

Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105                                 


<210>  9
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-1 VH

<400>  9
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
                5                   10                  15

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30

Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met
        35                  40                  45

Gly Trp Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe
    50                  55                  60

Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr
65                  70                  75                  80

Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95

Ala Arg Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr 
            100                 105                 110

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120

     
<210>  10
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-1 scFv

<400>  10
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
                5                   10                  15

Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45

Tyr Ser Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60

Ser Gly Pro Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80

Glu Asp Phe Ala Val Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Ser Thr Ser
            100                 105                 110

Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val 
        115                 120                 125

Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val 
    130                 135                 140

Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Met 
145                 150                 155                 160

His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Trp 
                165                 170                 175

Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe Gln Gly 
            180                 185                 190

Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
        195                 200                 205

Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
    210                 215                 220

Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr Trp Gly 
225                 230                 235                 240

Gln Gly Thr Leu Val Thr Val Ser Ser
                245     


<210>  11
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-2 VL

<400>  11
Asp Val Val Met Thr Gln Ser Pro Ala Phe Leu Ser Val Thr Pro Gly
              5                   10                  15

Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ala Pro Lys Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60

Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala
65                  70                  75                  80

Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105                 


<210>  12
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-2 VH

<400>  12
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
              5                   10                  15

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30

Tyr Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met
        35                  40                  45

Gly Trp Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe
    50                  55                  60

Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr
65                  70                  75                  80

Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95

Ala Arg Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr
            100                 105                 110

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120  
                

<210>  13
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-2 scFv

<400>  13
Asp Val Val Met Thr Gln Ser Pro Ala Phe Leu Ser Val Thr Pro Gly
                5                   10                  15

Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ala Pro Lys Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60

Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala
65                  70                  75                  80

Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Ser Thr Ser
            100                 105                 110

Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val 
        115                 120                 125

Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val 
    130                 135                 140

Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Tyr Met 
145                 150                 155                 160

His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Trp 
                165                 170                 175

Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe Gln Gly 
            180                 185                 190

Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
        195                 200                 205

Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
    210                 215                 220

Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr Trp Gly
225                 230                 235                 240

Gln Gly Thr Leu Val Thr Val Ser Ser
                245                                

     
<210>  14
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-3 VL

<400>  14
Asp Val Val Met Thr Gln Ser Pro Ala Phe Leu Ser Val Thr Pro Gly
                5                   10                  15

Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ala Pro Lys Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60

Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala
65                  70                  75                  80

Glu Asp Ala Ala Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105                 


<210>  15
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-3 VH

<400>  15
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala 
                5                   10                  15

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30

Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met
        35                  40                  45

Gly Lys Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe
    50                  55                  60

Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr
65                  70                  75                  80

Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95

Ala Arg Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr
            100                 105                 110

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120                


<210>  16
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-3 scFv

<400>  16
Asp Val Val Met Thr Gln Ser Pro Ala Phe Leu Ser Val Thr Pro Gly
                5                   10                  15

Glu Lys Val Thr Ile Thr Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Asp Gln Ala Pro Lys Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Val Pro Ser Arg Phe Ser Gly
    50                  55                  60

Ser Gly Ser Gly Thr Asp Phe Thr Phe Thr Ile Ser Ser Leu Glu Ala
65                  70                  75                  80

Glu Asp Ala Ala Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Ser Thr Ser
            100                 105                 110

Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val 
        115                 120                 125

Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val 
    130                 135                 140

Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met 
145                 150                 155                 160

His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Lys 
                165                 170                 175

Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe Gln Gly 
            180                 185                 190

Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
        195                 200                 205

Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
    210                 215                 220

Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr Trp Gly 
225                 230                 235                 240

Gln Gly Thr Leu Val Thr Val Ser Ser
                245                                


<210>  17
<211>  108
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-4 VL

<400>  17
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
                5                   10                  15

Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60

Ser Gly Pro Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80

Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg
            100                 105                 
                

<210>  18
<211>  123
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-4 VH

<400>  18
Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala
                5                   10                  15

Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30

Trp Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met
        35                  40                  45

Gly Lys Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe
    50                  55                  60

Gln Gly Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr
65                  70                  75                  80

Met Glu Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys
                85                  90                  95

Ala Arg Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr
            100                 105                 110

Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser
        115                 120                

     
<210>  19
<211>  249
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  h189-4 scFv

<400>  19
Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser Leu Ser Pro Gly
                5                   10                  15

Glu Arg Ala Thr Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn
            20                  25                  30

Leu His Trp Tyr Gln Gln Lys Pro Gly Gln Ala Pro Arg Leu Leu Ile
        35                  40                  45

Lys Ser Ala Ser Gln Ser Ile Ser Gly Ile Pro Ala Arg Phe Ser Gly
    50                  55                  60

Ser Gly Pro Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Glu Pro
65                  70                  75                  80

Glu Asp Phe Ala Val Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr
                85                  90                  95

Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Arg Gly Ser Thr Ser
            100                 105                 110

Gly Ser Gly Lys Pro Gly Ser Gly Glu Gly Ser Thr Lys Gly Gln Val 
        115                 120                 125

Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ala Ser Val 
    130                 135                 140

Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Trp Met
145                 150                 155                 160

His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Met Gly Lys 
                165                 170                 175

Ile Asn Pro Ser Asn Gly Arg Thr Asn Tyr Ala Gln Lys Phe Gln Gly 
            180                 185                 190

Arg Val Thr Met Thr Arg Asp Thr Ser Ile Ser Thr Ala Tyr Met Glu 
        195                 200                 205

Leu Ser Arg Leu Arg Ser Asp Asp Thr Ala Val Tyr Tyr Cys Ala Arg 
    210                 215                 220

Gly Gly Val Tyr Tyr Asp Leu Tyr Tyr Tyr Ala Leu Asp Tyr Trp Gly 
225                 230                 235                 240

Gln Gly Thr Leu Val Thr Val Ser Ser
                245                                


<210>  20
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  h189-1 scFv

<400>  20
gaaattgtgc tcactcaaag tcctgcaacc cttagcctgt ctccaggaga gcgagccact     60

ctgtcttgca gagccagcca atctatctct aataacctgc actggtatca acagaagcct    120

ggtcaggcac ctaggctcct gatatattcc gcgtctcagt ccatatcagg catccctgct    180

cgattcagcg gctctggtcc cggtactgat tttacgttga ctataagcag tcttgaacca    240

gaggattttg cagtttacta ttgtcagcag tcaaacagct ggccttatac cttcggccag    300

ggaaccaagg tagaaatcaa acgcggaagt acgtccggct caggaaagcc aggatctgga    360

gagggatcaa ctaagggaca agtgcagctg gtgcagtcag gagctgaagt taagaagcca    420

ggagcctccg tcaaggtctc ctgcaaggcc agcgggtata ctttcacgtc ctattatatg    480

cactgggtga ggcaagctcc tggtcaggga ttggagtgga tgggctggat caacccttca    540

aacggacgca ccaactacgc acagaaattc caaggtcggg taaccatgac gagagacacg    600

agtattagca cggcatatat ggagttgtct cgactgagga gcgacgacac ggccgtgtac    660

tattgcgcgc gaggtggggt gtactatgac ctgtattatt acgcattgga ctattggggg    720

cagggaacac tcgtcactgt ttccagc                                        747

               
<210>  21
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  h189-2 scFv

<400>  21
gatgtcgtta tgacgcaaag cccagccttt ctctccgtaa cgcctggcga aaaagttacg     60

atcacctgcc gggcatctca aagtatcagt aacaatttgc attggtatca acagaaaccc    120

gaccaagccc caaaactcct tatcaagagc gctagtcaga gtatctccgg cgtcccttct    180

cgattctctg gctcaggtag tggaactgat ttcaccttca ctatatctag tttggaagct    240

gaagacgcgg ccacttacta ctgtcagcag tcaaatagtt ggccctacac cttcggtcaa    300

gggacaaaag tcgagatcaa acgaggctca acctctggta gcgggaagcc tggatctgga    360

gaaggcagca caaagggaca ggtgcagttg gtccaatctg gggcggaggt taagaagcct    420

ggggcttctg ttaaagtgag ttgtaaggca tcaggttata ctttcacatc ctactacatg    480

cactgggttc ggcaggctcc gggccaggga cttgaatgga tggggtggat taatccaagc    540

aatggccgca ctaactatgc tcagaagttc caaggaaggg tcacaatgac tagagacact    600

tccatatcaa ccgcgtatat ggaactttca cgacttcgca gcgacgatac tgcggtctat    660

tattgtgcgc gagggggagt ttattatgac ctttactact acgcgcttga ttattgggga    720

cagggtaccc ttgttaccgt ttccagc                                        747


<210>  22
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  h189-3 scFv

<400>  22
gatgtcgtta tgacgcaaag cccagccttt ctctccgtaa cgcctggcga aaaagttacg     60

atcacctgcc gggcatctca aagtatcagt aacaatttgc attggtatca acagaaaccc    120

gaccaagccc caaaactcct tatcaagagc gctagtcaga gtatctccgg cgtcccttct    180

cgattctctg gctcaggtag tggaactgat ttcaccttca ctatatctag tttggaagct    240

gaagacgcgg ccatgtactt ctgtcagcag tcaaatagtt ggccctacac cttcggtcaa    300

gggacaaaag tcgagatcaa acgaggctca acctctggta gcgggaagcc tggatctgga    360

gaaggcagca caaagggaca ggtgcagttg gtccaatctg gggcggaggt taagaagcct    420

ggggcttctg ttaaagtgag ttgtaaggca tcaggttata ctttcacatc ctactggatg    480

cactgggttc ggcaggctcc gggccaggga cttgaatgga tggggaaaat taatccaagc    540

aatggccgca ctaactatgc tcagaagttc caaggaaggg tcacaatgac tagagacact    600

tccatatcaa ccgcgtatat ggaactttca cgacttcgca gcgacgatac tgcggtctat    660

tattgtgcgc gagggggagt ttattatgac ctttactact acgcgcttga ttattgggga    720

cagggtaccc ttgttaccgt ttccagc                                        747


<210>  23
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  h189-4 scFv

<400>  23
gaaatcgtgt tgacacagag ccctgcaacc ctttcacttt ccccaggcga acgcgcgacc     60

ctgtcatgcc gcgctagtca aagtatatca aataacctcc actggtatca gcaaaagcct    120

ggtcaggccc cacgcttgtt gatcaaatct gcctctcaaa gcatcagtgg tattcccgct    180

agattctctg gtagcgggcc tgggaccgat ttcactttga ctatctcatc tttggaaccg    240

gaggatttcg ctgtctactt ttgccaacag agtaattcat ggccttatac gttcggacag    300

ggaacgaagg tagagattaa aagaggctct acaagcggtt ccggtaagcc gggctctggg    360

gaaggttcaa cgaaagggca agtacagctc gtacagtccg gggcagaagt taagaagccg    420

ggggcatccg tcaaagtatc atgtaaagcg agtggttata cgttcacaag ctactggatg    480

cattgggtac gccaagcccc aggccaagga ttggaatgga tgggtaagat taacccgagc    540

aatggaagga caaattacgc tcaaaagttc cagggacggg tcacaatgac gcgcgatacg    600

agcattagca cagcctacat ggagctttcc cgccttaggt cagatgacac tgctgtgtat    660

tattgcgcca gaggtggagt gtattacgac ctctattatt acgccctcga ctattgggga    720

cagggtactc ttgttactgt gagtagc                                        747


<210>  24
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α跨膜结构域

<400>  24
Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
                5                   10                  15

Ser Leu Val Ile Thr Leu Tyr Cys Lys
            20                  25


<210>  25
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α跨膜结构域

<400>  25
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc     60

accctttact gcaaa                                                      75


<210>  26
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD28跨膜结构域

<400>  26
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu
                5                   10                  15

Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val
            20                  25


<210>  27
<211>  81
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD28跨膜结构域

<400>  27
ttttgggtcc tcgtcgtagt tggaggggta cttgcctgtt atagcctcct ggttaccgta     60

gcatttatta tattctgggt g                                               81


<210>  28
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD28共刺激结构域

<400>  28
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
                5                   10                  15

Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro
            20                  25                  30

Pro Arg Asp Phe Ala Ala Tyr Arg Ser
        35                  40


<210>  29
<211>  123
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD28共刺激结构域

<400>  29
aggagtaaga ggagcaggct cctgcacagt gactacatga acatgactcc ccgccgcccc     60

gggcccaccc gcaagcatta ccagccctat gccccaccac gcgacttcgc agcctatcgc    120

tcc                                                                  123


<210>  30
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  4-1BB共刺激结构域

<400>  30
Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg 
                5                   10                  15

Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro
            20                  25                  30

Glu Glu Glu Glu Gly Gly Cys Glu
        35                  40


<210>  31
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  4-1BB共刺激结构域

<400>  31
cggggcagaa agaaactcct gtatatattc aaacaaccat ttatgagacc agtacaaact     60

actcaagagg aagatggctg tagctgccga tttccagaag aagaagaagg aggatgtgaa     120


<210>  32
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD3ζ信号传导结构域

<400>  32
Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
                5                   10                  15

Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu
            20                  25                  30

Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly
        35                  40                  45

Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60

Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu
65                  70                  75                  80

Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr
                85                  90                  95

Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro
            100                 105                 110

Arg


<210>  33
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD3ζ信号传导结构域

<400>  33
ctgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag     60

ctctataacg agctcaatct aggacgaaga gaggagtacg atgttttgga caagagacgt    120

ggccgggacc ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac    180

aatgaactgc agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag    240

cgccggaggg gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac    300

acctacgacg cccttcacat gcaggccctg ccccctcgc                           339


<210>  34
<211>  114
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD3ζ信号传导结构域

<400>  34
Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln
                5                   10                  15

Gly Gln Asn Gln Leu Phe Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu
            20                  25                  30

Phe Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly
        35                  40                  45

Lys Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu
    50                  55                  60

Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly
65                  70                  75                  80

Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Phe Gln Gly Leu Ser
                85                  90                  95

Thr Ala Thr Lys Asp Thr Phe Asp Ala Leu His Met Gln Ala Leu Pro
            100                 105                 110

Pro Arg


<210>  35
<211>  342
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD3ζ信号传导结构域

<400>  35
ctgagagtga agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag     60

ctctttaacg agctcaatct aggacgaaga gaggagttcg atgttttgga caagagacgt    120

ggccgggacc ctgagatggg gggaaagccg cagagaagga agaaccctca ggaaggcctg    180

tacaatgaac tgcagaaaga taagatggcg gaggcctaca gtgagattgg gatgaaaggc    240

gagcgccgga ggggcaaggg gcacgatggc cttttccagg gtctcagtac agccaccaag    300

gacacctttg acgcccttca catgcaggcc ctgccccctc gc                       342


<210>  36
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  B2M信号肽

<400>  36
Met Ser Arg Ser Val Ala Leu Ala Val Leu Ala Leu Leu Ser Leu Ser
                5                   10                  15

Gly Leu Glu Ala
            20


<210>  37
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  B2M信号肽

<400>  37
atgtcccgct ctgttgcttt ggctgtgctg gcccttttgt cccttagcgg actggaggcc     60


<210>  38
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α信号肽

<400>  38
Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu
                5                   10                  15

His Ala Ala Arg Pro
            20


<210>  39
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α信号肽

<400>  39
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg     60

ccg                                                                   63


<210>  40
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α铰链区

<400>  40
Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala
                5                   10                  15

Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly
            20                  25                  30

Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp
        35                  40                  45


<210>  41
<211>  135
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD8α铰链区

<400>  41
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg     60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg    120

gacttcgcct gtgat                                                     135


<210>  42
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  CD28铰链区

<400>  42
Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn
                5                   10                  15

Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu
            20                  25                  30

Phe Pro Gly Pro Ser Lys Pro
        35


<210>  43
<211>  117
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  CD28铰链区

<400>  43
attgaagtta tgtatcctcc tccttaccta gacaatgaga agagcaatgg aaccattatc     60

catgtgaaag ggaaacacct ttgtccaagt cccctatttc ccggaccttc taagccc       117


<210>  44
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<221>
<223>  IgG4铰链区

<400>  44
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
                5                   10


<210>  45
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<221>
<223>  IgG4铰链区

<400>  45
gaaagcaaat acgggccgcc gtgtccaccc tgtccg                               36

