                         SEQUENCE LISTING

<110>  The Trustees of Princeton University
       Zarzhitsky, Shlomo
       Hecht, Michael
 
<120>  METHODS OF CONVERTING PRECURSOR PROTEINS TO MATURE PROTEINS USING
        KEX2 PROTEASES

<130>  5391.1030001

<150>  63/106,676
<151>  2020-10-28

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  814
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Lys Val Arg Lys Tyr Ile Thr Leu Cys Phe Trp Trp Ala Phe Ser 
1               5                   10                  15      


Thr Ser Ala Leu Val Ser Ser Gln Gln Ile Pro Leu Lys Asp His Thr 
            20                  25                  30          


Ser Arg Gln Tyr Phe Ala Val Glu Ser Asn Glu Thr Leu Ser Arg Leu 
        35                  40                  45              


Glu Glu Met His Pro Asn Trp Lys Tyr Glu His Asp Val Arg Gly Leu 
    50                  55                  60                  


Pro Asn His Tyr Val Phe Ser Lys Glu Leu Leu Lys Leu Gly Lys Arg 
65                  70                  75                  80  


Ser Ser Leu Glu Glu Leu Gln Gly Asp Asn Asn Asp His Ile Leu Ser 
                85                  90                  95      


Val His Asp Leu Phe Pro Arg Asn Asp Leu Phe Lys Arg Leu Pro Val 
            100                 105                 110         


Pro Ala Pro Pro Met Asp Ser Ser Leu Leu Pro Val Lys Glu Ala Glu 
        115                 120                 125             


Asp Lys Leu Ser Ile Asn Asp Pro Leu Phe Glu Arg Gln Trp His Leu 
    130                 135                 140                 


Val Asn Pro Ser Phe Pro Gly Ser Asp Ile Asn Val Leu Asp Leu Trp 
145                 150                 155                 160 


Tyr Asn Asn Ile Thr Gly Ala Gly Val Val Ala Ala Ile Val Asp Asp 
                165                 170                 175     


Gly Leu Asp Tyr Glu Asn Glu Asp Leu Lys Asp Asn Phe Cys Ala Glu 
            180                 185                 190         


Gly Ser Trp Asp Phe Asn Asp Asn Thr Asn Leu Pro Lys Pro Arg Leu 
        195                 200                 205             


Ser Asp Asp Tyr His Gly Thr Arg Cys Ala Gly Glu Ile Ala Ala Lys 
    210                 215                 220                 


Lys Gly Asn Asn Phe Cys Gly Val Gly Val Gly Tyr Asn Ala Lys Ile 
225                 230                 235                 240 


Ser Gly Ile Arg Ile Leu Ser Gly Asp Ile Thr Thr Glu Asp Glu Ala 
                245                 250                 255     


Ala Ser Leu Ile Tyr Gly Leu Asp Val Asn Asp Ile Tyr Ser Cys Ser 
            260                 265                 270         


Trp Gly Pro Ala Asp Asp Gly Arg His Leu Gln Gly Pro Ser Asp Leu 
        275                 280                 285             


Val Lys Lys Ala Leu Val Lys Gly Val Thr Glu Gly Arg Asp Ser Lys 
    290                 295                 300                 


Gly Ala Ile Tyr Val Phe Ala Ser Gly Asn Gly Gly Thr Arg Gly Asp 
305                 310                 315                 320 


Asn Cys Asn Tyr Asp Gly Tyr Thr Asn Ser Ile Tyr Ser Ile Thr Ile 
                325                 330                 335     


Gly Ala Ile Asp His Lys Asp Leu His Pro Pro Tyr Ser Glu Gly Cys 
            340                 345                 350         


Ser Ala Val Met Ala Val Thr Tyr Ser Ser Gly Ser Gly Glu Tyr Ile 
        355                 360                 365             


His Ser Ser Asp Ile Asn Gly Arg Cys Ser Asn Ser His Gly Gly Thr 
    370                 375                 380                 


Ser Ala Ala Ala Pro Leu Ala Ala Gly Val Tyr Thr Leu Leu Leu Glu 
385                 390                 395                 400 


Ala Asn Pro Asn Leu Thr Trp Arg Asp Val Gln Tyr Leu Ser Ile Leu 
                405                 410                 415     


Ser Ala Val Gly Leu Glu Lys Asn Ala Asp Gly Asp Trp Arg Asp Ser 
            420                 425                 430         


Ala Met Gly Lys Lys Tyr Ser His Arg Tyr Gly Phe Gly Lys Ile Asp 
        435                 440                 445             


Ala His Lys Leu Ile Glu Met Ser Lys Thr Trp Glu Asn Val Asn Ala 
    450                 455                 460                 


Gln Thr Trp Phe Tyr Leu Pro Thr Leu Tyr Val Ser Gln Ser Thr Asn 
465                 470                 475                 480 


Ser Thr Glu Glu Thr Leu Glu Ser Val Ile Thr Ile Ser Glu Lys Ser 
                485                 490                 495     


Leu Gln Asp Ala Asn Phe Lys Arg Ile Glu His Val Thr Val Thr Val 
            500                 505                 510         


Asp Ile Asp Thr Glu Ile Arg Gly Thr Thr Thr Val Asp Leu Ile Ser 
        515                 520                 525             


Pro Ala Gly Ile Ile Ser Asn Leu Gly Val Val Arg Pro Arg Asp Val 
    530                 535                 540                 


Ser Ser Glu Gly Phe Lys Asp Trp Thr Phe Met Ser Val Ala His Trp 
545                 550                 555                 560 


Gly Glu Asn Gly Val Gly Asp Trp Lys Ile Lys Val Lys Thr Thr Glu 
                565                 570                 575     


Asn Gly His Arg Ile Asp Phe His Ser Trp Arg Leu Lys Leu Phe Gly 
            580                 585                 590         


Glu Ser Ile Asp Ser Ser Lys Thr Glu Thr Phe Val Phe Gly Asn Asp 
        595                 600                 605             


Lys Glu Glu Val Glu Pro Ala Ala Thr Glu Ser Thr Val Ser Gln Tyr 
    610                 615                 620                 


Ser Ala Ser Ser Thr Ser Ile Ser Ile Ser Ala Thr Ser Thr Ser Ser 
625                 630                 635                 640 


Ile Ser Ile Gly Val Glu Thr Ser Ala Ile Pro Gln Thr Thr Thr Ala 
                645                 650                 655     


Ser Thr Asp Pro Asp Ser Asp Pro Asn Thr Pro Lys Lys Leu Ser Ser 
            660                 665                 670         


Pro Arg Gln Ala Met His Tyr Phe Leu Thr Ile Phe Leu Ile Gly Ala 
        675                 680                 685             


Thr Phe Leu Val Leu Tyr Phe Met Phe Phe Met Lys Ser Arg Arg Arg 
    690                 695                 700                 


Ile Arg Arg Ser Arg Ala Glu Thr Tyr Glu Phe Asp Ile Ile Asp Thr 
705                 710                 715                 720 


Asp Ser Glu Tyr Asp Ser Thr Leu Asp Asn Gly Thr Ser Gly Ile Thr 
                725                 730                 735     


Glu Pro Glu Glu Val Glu Asp Phe Asp Phe Asp Leu Ser Asp Glu Asp 
            740                 745                 750         


His Leu Ala Ser Leu Ser Ser Ser Glu Asn Gly Asp Ala Glu His Thr 
        755                 760                 765             


Ile Asp Ser Val Leu Thr Asn Glu Asn Pro Phe Ser Asp Pro Ile Lys 
    770                 775                 780                 


Gln Lys Phe Pro Asn Asp Ala Asn Ala Glu Ser Ala Ser Asn Lys Leu 
785                 790                 795                 800 


Gln Glu Leu Gln Pro Asp Val Pro Pro Ser Ser Gly Arg Ser 
                805                 810                 


<210>  2
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP1

<400>  2

Met Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg Gly Gly Ser Gly Gly Ser Arg 
            100                 105                 


<210>  3
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP1

<400>  3

Met Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg 
            100         


<210>  4
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP1

<400>  4

Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  5
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP1

<400>  5

Asp Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  6
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP2

<400>  6

Met Tyr Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


His Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg Gly Gly Ser Gly Gly Ser Arg 
            100                 105                 


<210>  7
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP2

<400>  7

Met Tyr Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


His Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg 
            100         


<210>  8
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP2

<400>  8

Tyr Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu His 
1               5                   10                  15      


Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  9
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP2

<400>  9

Asp Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu His 
1               5                   10                  15      


Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  10
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP3

<400>  10

Met Tyr Gly Lys Cys Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Cys Val His Arg Gly Gly Ser Gly Gly Ser Arg 
            100                 105                 


<210>  11
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP3

<400>  11

Met Tyr Gly Lys Cys Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Cys Val His Arg 
            100         


<210>  12
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP3

<400>  12

Tyr Gly Lys Cys Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Cys Val His Arg 
            100     


<210>  13
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP3

<400>  13

Asp Gly Lys Cys Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Cys Val His Arg 
            100     


<210>  14
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP4

<400>  14

Met Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu His His His His His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg Gly Gly Ser Gly Gly Ser Arg 
            100                 105                 


<210>  15
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP4

<400>  15

Met Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu 
1               5                   10                  15      


Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp 
            20                  25                  30          


Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met 
        35                  40                  45              


Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe 
    50                  55                  60                  


Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu His His His His His 
65                  70                  75                  80  


Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu 
                85                  90                  95      


Glu Glu Leu Val His Arg 
            100         


<210>  16
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP4

<400>  16

Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu His His His His His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  17
<211>  101
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP4

<400>  17

Asp Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu Lys 
1               5                   10                  15      


Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp Val 
            20                  25                  30          


Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln 
        35                  40                  45              


Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe Gln 
    50                  55                  60                  


Gln Val Leu Asp Glu Leu Asn Asn His Leu His His His His His Thr 
65                  70                  75                  80  


Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu Glu 
                85                  90                  95      


Glu Leu Val His Arg 
            100     


<210>  18
<211>  110
<212>  PRT
<213>  Homo sapiens

<400>  18

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly 
            20                  25                  30          


Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe 
        35                  40                  45              


Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val Gly 
    50                  55                  60                  


Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu 
65                  70                  75                  80  


Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys Cys 
                85                  90                  95      


Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
            100                 105                 110 


<210>  19
<211>  24
<212>  PRT
<213>  Homo sapiens

<400>  19

Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu 
1               5                   10                  15      


Trp Gly Pro Asp Pro Ala Ala Ala 
            20                  


<210>  20
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  20

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30  


<210>  21
<211>  31
<212>  PRT
<213>  Homo sapiens

<400>  21

Glu Ala Glu Asp Leu Gln Val Gly Gln Val Glu Leu Gly Gly Gly Pro 
1               5                   10                  15      


Gly Ala Gly Ser Leu Gln Pro Leu Ala Leu Glu Gly Ser Leu Gln 
            20                  25                  30      


<210>  22
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  22

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Asn 
            20      


<210>  23
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glargine A Chain

<400>  23

Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu 
1               5                   10                  15      


Glu Asn Tyr Cys Gly 
            20      


<210>  24
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Glargine B Chain

<400>  24

Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr 
1               5                   10                  15      


Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr Arg Arg 
            20                  25                  30          


<210>  25
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP-Insulin

<400>  25

Met Lys Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala 
1               5                   10                  15      


Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr 
            20                  25                  30          


Arg Arg Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val 
        35                  40                  45              


Leu Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His 
    50                  55                  60                  


Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe 
65                  70                  75                  80  


Met Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu 
                85                  90                  95      


Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys 
            100                 105                 110         


His Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His 
        115                 120                 125             


Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln Cys Cys 
    130                 135                 140                 


Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn 
145                 150                 155             


<210>  26
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP-glargine

<400>  26

Met His Lys Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
1               5                   10                  15      


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
            20                  25                  30          


Lys Thr Arg Arg Asp Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln 
        35                  40                  45              


Glu Val Leu Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn 
    50                  55                  60                  


Leu His Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His 
65                  70                  75                  80  


Asp Phe Met Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met 
                85                  90                  95      


Lys Glu Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly 
            100                 105                 110         


Gly Lys His Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe 
        115                 120                 125             


His His Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln 
    130                 135                 140                 


Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
145                 150                 155                 160 


<210>  27
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP-glargine

<400>  27

Met His Lys Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
1               5                   10                  15      


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
            20                  25                  30          


Lys Thr Arg Arg Asp Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln 
        35                  40                  45              


Glu Val Leu His Asn Leu His His Asn Trp His Gly Gly His Asp Asn 
    50                  55                  60                  


Leu His Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His 
65                  70                  75                  80  


Asp Phe Met Gln Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met 
                85                  90                  95      


His Glu Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly 
            100                 105                 110         


Gly His His Thr Val His His Ile Glu Gln Asn Ile His Glu Ile Phe 
        115                 120                 125             


His His Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln 
    130                 135                 140                 


Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
145                 150                 155                 160 


<210>  28
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP-glargine

<400>  28

Met His Lys Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
1               5                   10                  15      


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
            20                  25                  30          


Lys Thr Arg Arg Asp Gly Lys Cys Asn Asp Leu Leu Glu Asp Leu Gln 
        35                  40                  45              


Glu Val Leu Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn 
    50                  55                  60                  


Leu His Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His 
65                  70                  75                  80  


Asp Phe Met Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met 
                85                  90                  95      


Lys Glu Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly 
            100                 105                 110         


Gly Lys His Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe 
        115                 120                 125             


His His Leu Glu Glu Cys Val His Arg Lys Arg Gly Ile Val Glu Gln 
    130                 135                 140                 


Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
145                 150                 155                 160 


<210>  29
<211>  160
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DEEP-glargine

<400>  29

Met His Lys Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val 
1               5                   10                  15      


Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro 
            20                  25                  30          


Lys Thr Arg Arg Asp Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln 
        35                  40                  45              


Glu Val Leu Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn 
    50                  55                  60                  


Leu His Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His 
65                  70                  75                  80  


Asp Phe Met Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met 
                85                  90                  95      


Lys Glu Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu His His 
            100                 105                 110         


His His His Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe 
        115                 120                 125             


His His Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln 
    130                 135                 140                 


Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Gly 
145                 150                 155                 160 


