                         SEQUENCE LISTING

<110>  The Government of the United States of America, as 
       represented by the Secretary of the Navy
 
<120>  Single Domain Antibodies to SARS-CoV-2 Nucleocapsid Protein

<130>  113715

<150>  63107595
<151>  2020-10-30

<160>  17    

<170>  PatentIn version 3.5

<210>  1
<211>  127
<212>  PRT
<213>  Lama glama

<400>  1

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Ala Val Ser Gly Arg Thr Ile Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Glu Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Gly Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  2
<211>  121
<212>  PRT
<213>  Lama glama

<400>  2

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Arg Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Met Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Ala Ile Asn Gly Gly Gly Gly Ser Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Tyr Gln Ala Ala Val His Gln Glu Lys Glu Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  3
<211>  126
<212>  PRT
<213>  Lama glama

<400>  3

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Asp Ser Thr Gln 
            20                  25                  30          


His Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Gln Trp Arg Gly Gly Gly Thr Ser Tyr Thr Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asn Thr Arg Trp Thr Tyr Phe Ser Pro Thr Val Pro Asp Arg 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  4
<211>  127
<212>  PRT
<213>  Lama glama

<400>  4

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ala Arg Thr Phe Tyr Thr Met 
            20                  25                  30          


Gly Trp Phe Arg Gln Val Leu Gly Lys Asp Arg Glu Phe Val Gly Ala 
        35                  40                  45              


Ile Arg Trp Gly Val Tyr Ala Thr Thr Arg Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Ser Ile Ser Arg Asp Asp Ala Thr Asn Thr Val Ala Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Ala Arg Ala Gly Pro Leu Gly Phe Glu Leu Ser Ala Thr Ser Ser Ala 
            100                 105                 110         


Glu Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  5
<211>  119
<212>  PRT
<213>  Lama glama

<400>  5

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Glu 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Val Val Ser Gly Gly Ala Phe Arg Ile Met 
            20                  25                  30          


Asp Met Gly Trp Phe Arg Gln Val Pro Gly Lys Gln Arg Glu Val Val 
        35                  40                  45              


Gly Val Ile Ser Ser Gly Gly Tyr Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu 
65                  70                  75                  80  


His Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ile Ile Pro Lys Ser Asp Gln Gly Ala Val Asn Thr Trp Gly Lys Gly 
            100                 105                 110         


Thr Leu Val Ser Val Ser Ser 
        115                 


<210>  6
<211>  127
<212>  PRT
<213>  Lama glama

<400>  6

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Val Ser Gly Arg Thr Thr Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  7
<211>  127
<212>  PRT
<213>  Lama glama

<400>  7

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Val Ser Gly Arg Thr Thr Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  8
<211>  127
<212>  PRT
<213>  Lama glama

<400>  8

Asp Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Val Ser Gly Arg Thr Ile Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Ala Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  9
<211>  127
<212>  PRT
<213>  Lama glama

<400>  9

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Val Ser Gly Arg Thr Thr Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Ser Glu Asp Thr Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  10
<211>  127
<212>  PRT
<213>  Lama glama

<400>  10

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Leu Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ala Ser Gly Arg Thr Ile Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  11
<211>  127
<212>  PRT
<213>  Lama glama

<400>  11

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Leu Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ala Ser Gly Arg Thr Ile Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  12
<211>  127
<212>  PRT
<213>  Lama glama

<400>  12

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Asp 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ala Ser Gly Arg Thr Ile Ser Thr 
            20                  25                  30          


Phe Ala Met Gly Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe 
        35                  40                  45              


Val Ala Thr Ile Asn Trp Ser Gly Ser Ser Ala Arg Tyr Ala Asp Pro 
    50                  55                  60                  


Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ala Lys Asn Thr Val 
65                  70                  75                  80  


Tyr Leu Glu Met Ser Ser Leu Lys Pro Glu Asp Ser Ala Val Tyr Tyr 
                85                  90                  95      


Cys Ala Ser Gly Arg Tyr Leu Gly Gly Ile Thr Ser Tyr Ser Gln Gly 
            100                 105                 110         


Asp Phe Ala Pro Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125         


<210>  13
<211>  126
<212>  PRT
<213>  Lama glama

<400>  13

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Pro Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Asp Ser Thr Gln 
            20                  25                  30          


His Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Gln Trp Arg Gly Gly Gly Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Glu Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asn Thr Arg Trp Thr Tyr Phe Ser Pro Thr Val Pro Asp Arg 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  14
<211>  126
<212>  PRT
<213>  Lama glama

<400>  14

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Pro Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Asp Ser Thr Gln 
            20                  25                  30          


His Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Gln Trp Arg Gly Gly Gly Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Ser Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asn Thr Arg Trp Thr Tyr Phe Ser Pro Thr Val Pro Asp Arg 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  15
<211>  126
<212>  PRT
<213>  Lama glama

<400>  15

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Met Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Ser Leu Ser Cys Ala Ala Ser Gly Arg Thr Asp Ser Thr Gln 
            20                  25                  30          


His Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Gln Trp Arg Gly Gly Gly Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asn Thr Arg Trp Thr Tyr Phe Ser Pro Thr Val Pro Asp Arg 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  16
<211>  126
<212>  PRT
<213>  Lama glama

<400>  16

Glu Val Gln Leu Gln Ala Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Asp Ser Thr Gln 
            20                  25                  30          


His Met Ala Trp Phe Arg Gln Ala Pro Gly Lys Glu Arg Glu Phe Val 
        35                  40                  45              


Thr Ala Ile Gln Trp Arg Gly Gly Gly Thr Ser Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Glu Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Asn Thr Arg Trp Thr Tyr Phe Ser Pro Thr Val Pro Asp Arg 
            100                 105                 110         


Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120                 125     


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic construct

<400>  17

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


