               SEQUENCE LISTING

<110> Söderberg Ola

<120> New DNA polymerase and use thereof

<130> P42001903SE00

<150> SE2051265-3
<151> 2020-10-30

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 2946
<212> DNA
<213> Artificial Sequence


<220> 
<223> DNA polymerase

<400> 1
atgcatcacc atcaccatca cgaaaacctg tattttcagg gcatggttca gatcccccaa      60

aatccactta tccttgtaga tggttcatct tatctttatc gcgcatatca cgcgtttccc     120

ccgctgacta acagcgcagg cgagccgacc ggtgcgatgt atggtgtcct caacatgctg     180

cgcagtctga tcatgcaata taaaccgacg catgcagcgg tggtctttga cgccaaggga     240

aaaacctttc gtgatgaact gtttgaacat tacaaatcac atcgcccgcc aatgccggac     300

gatctgcgtg cacaaatcga acccttgcac gcgatggtta aagcgatggg actgccgctg     360

ctggcggttt ctggcgtaga agcggacgac gttatcggta ctctggcgcg cgaagccgaa     420

aaagccgggc gtccggtgct gatcagcact ggcgataaag atatggcgca gctggtgacg     480

ccaaatatta cgcttatcaa taccatgacg aataccatcc tcggaccgga agaggtggtg     540

aataagtacg gcgtgccgcc agaactgatc atcgatttcc tggcgctgat gggtgactcc     600

tctgataaca ttcctggcgt accgggcgtc ggtgaaaaaa ccgcgcaggc attgctgcaa     660

ggtcttggcg gactggatac gctgtatgcc gagccagaaa aaattgctgg gttgagcttc     720

cgtggcgcga aaacaatggc agcgaagctc gagcaaaaca aagaagttgc ttatctctca     780

taccagctgg cgacgattaa aaccgacgtt gaactggagc tgacctgtga acaactggaa     840

gtgcagcaac cggcagcgga agagttgttg gggctgttca aaaagtatga gttcaaacgc     900

tggactgctg atgtcgaagc gggcaaatgg ttacaggcca aaggggcaaa accagccgcg     960

aagccacagg aaaccagtgt tgcagacgaa gcaccagaag tgacggcaac gacaggcagc    1020

accgggtcga ctgggagtac gggttccatg tctaagttta catggaaaga gttaattcaa    1080

ttaggcagtc catcgaaagc atacgagtcc tcattagctt gtatcgcaca tattgatatg    1140

aatgcgttct tcgcccaggt ggagcagatg cgttgtggcc tgtctaagga ggatcccgta    1200

gtatgcgttc agtggaacag catcattgcg gtgtcttatg ctgctcgcaa atacggcatc    1260

tcccgtatgg acaccatcca ggaggctctg aagaaatgct cgaacttaat ccctattcat    1320

acggccgtct tcaagaaagg agaagatttc tggcagtacc atgatgggtg tgggtcgtgg    1380

gtacaggacc ccgcgaagca aatctcggtc gaggatcaca aggtttcact ggagccctat    1440

cgtcgtgaat cacgcaaggc gcttaaaatc ttcaagtcgg catgcgattt ggtagagcgt    1500

gcctctattg acgaggtatt ccttgacttg ggacgtatct gctttaacat gttaatgttt    1560

gacaatgagt acgaattgac aggggactta aagttaaaag atgcactgtc taatattcgc    1620

gaagccttta tcggggggaa ttatgatatt aactcgcatt taccgcttat tcctgagaaa    1680

attaagagct tgaagtttga gggggatgtt tttaatcccg aaggccgtga cctgatcacc    1740

gactgggacg acgtgattct tgcacttggg agccaggttt gcaaaggtat tcgcgacagt    1800

attaaagaca tcttgggcta tacaacctca tgcgggcttt catcaacgaa aaacgtctgt    1860

aaacttgctt caaactataa gaagcctgac gcccagacta ttgtcaagaa tgactgtctt    1920

ctggattttt tggactgcgg aaagttcgag attacatcct tttggacgct gggtggagtc    1980

ttgggaaagg aactgattga tgtccttgac ttacctcatg agaactcgat caaacacatt    2040

cgtgagacat ggcctgacaa cgccggacag ttgaaggagt ttctggacgc caaggtcaaa    2100

caatctgatt atgatcgctc gacctctaac atcgaccctt tgaaaaccgc tgatctggcc    2160

gaaaagcttt ttaaactttc gcgcggtcgt tacggacttc cattatcttc acgtccggtt    2220

gttaagtcta tgatgtccaa caaaaacctg cgtggtaagt cgtgcaattc catcgttgac    2280

tgtatttcct ggttagaagt attctgcgcc gagctgacat cccgcattca ggatcttgaa    2340

caagagtata acaagattgt catccctcgt acagtctcga tctcactgaa aactaaatcg    2400

tacgaagtgt accgtaagtc agggccggtg gcctacaagg gcatcaattt tcaaagccac    2460

gagttattga aagtcgggat caaatttgta accgaccttg acattaaagg gaaaaataaa    2520

tcctactatc cgttaacgaa gctgtctatg accattacta acttcgacat catcgatttg    2580

caaaaaactg ttgtggacat gtttgggaac caagtacaca catttaagtc ctcggcgggc    2640

aaagaggacg aggagaagac aactagcagt aaggcggatg aaaagacacc gaaactggaa    2700

tgttgtaaat atcaagtgac tttcacggac caaaaagcac ttcaagagca tgctgactac    2760

cacctggctt taaagctttc tgagggtctg aatggagcgg aggagagcag taaaaatttg    2820

tcctttggcg aaaagcgctt gctgttctct cgtaaacgtc caaacagtca acacactgct    2880

actccccaga aaaagcaggt tacttcatca aaaaatattc ttagtttctt cactcgtaaa    2940

aaatga                                                               2946


<210> 2
<211> 981
<212> PRT
<213> Artificial Sequence


<220> 
<223> DNA polymerase

<400> 2
Met His His His His His His Glu Asn Leu Tyr Phe Gln Gly Met Val 
1               5                   10                  15      
Gln Ile Pro Gln Asn Pro Leu Ile Leu Val Asp Gly Ser Ser Tyr Leu 
            20                  25                  30          
Tyr Arg Ala Tyr His Ala Phe Pro Pro Leu Thr Asn Ser Ala Gly Glu 
        35                  40                  45              
Pro Thr Gly Ala Met Tyr Gly Val Leu Asn Met Leu Arg Ser Leu Ile 
    50                  55                  60                  
Met Gln Tyr Lys Pro Thr His Ala Ala Val Val Phe Asp Ala Lys Gly 
65                  70                  75                  80  
Lys Thr Phe Arg Asp Glu Leu Phe Glu His Tyr Lys Ser His Arg Pro 
                85                  90                  95      
Pro Met Pro Asp Asp Leu Arg Ala Gln Ile Glu Pro Leu His Ala Met 
            100                 105                 110         
Val Lys Ala Met Gly Leu Pro Leu Leu Ala Val Ser Gly Val Glu Ala 
        115                 120                 125             
Asp Asp Val Ile Gly Thr Leu Ala Arg Glu Ala Glu Lys Ala Gly Arg 
    130                 135                 140                 
Pro Val Leu Ile Ser Thr Gly Asp Lys Asp Met Ala Gln Leu Val Thr 
145                 150                 155                 160 
Pro Asn Ile Thr Leu Ile Asn Thr Met Thr Asn Thr Ile Leu Gly Pro 
                165                 170                 175     
Glu Glu Val Val Asn Lys Tyr Gly Val Pro Pro Glu Leu Ile Ile Asp 
            180                 185                 190         
Phe Leu Ala Leu Met Gly Asp Ser Ser Asp Asn Ile Pro Gly Val Pro 
        195                 200                 205             
Gly Val Gly Glu Lys Thr Ala Gln Ala Leu Leu Gln Gly Leu Gly Gly 
    210                 215                 220                 
Leu Asp Thr Leu Tyr Ala Glu Pro Glu Lys Ile Ala Gly Leu Ser Phe 
225                 230                 235                 240 
Arg Gly Ala Lys Thr Met Ala Ala Lys Leu Glu Gln Asn Lys Glu Val 
                245                 250                 255     
Ala Tyr Leu Ser Tyr Gln Leu Ala Thr Ile Lys Thr Asp Val Glu Leu 
            260                 265                 270         
Glu Leu Thr Cys Glu Gln Leu Glu Val Gln Gln Pro Ala Ala Glu Glu 
        275                 280                 285             
Leu Leu Gly Leu Phe Lys Lys Tyr Glu Phe Lys Arg Trp Thr Ala Asp 
    290                 295                 300                 
Val Glu Ala Gly Lys Trp Leu Gln Ala Lys Gly Ala Lys Pro Ala Ala 
305                 310                 315                 320 
Lys Pro Gln Glu Thr Ser Val Ala Asp Glu Ala Pro Glu Val Thr Ala 
                325                 330                 335     
Thr Thr Gly Ser Thr Gly Ser Thr Gly Ser Thr Gly Ser Met Ser Lys 
            340                 345                 350         
Phe Thr Trp Lys Glu Leu Ile Gln Leu Gly Ser Pro Ser Lys Ala Tyr 
        355                 360                 365             
Glu Ser Ser Leu Ala Cys Ile Ala His Ile Asp Met Asn Ala Phe Phe 
    370                 375                 380                 
Ala Gln Val Glu Gln Met Arg Cys Gly Leu Ser Lys Glu Asp Pro Val 
385                 390                 395                 400 
Val Cys Val Gln Trp Asn Ser Ile Ile Ala Val Ser Tyr Ala Ala Arg 
                405                 410                 415     
Lys Tyr Gly Ile Ser Arg Met Asp Thr Ile Gln Glu Ala Leu Lys Lys 
            420                 425                 430         
Cys Ser Asn Leu Ile Pro Ile His Thr Ala Val Phe Lys Lys Gly Glu 
        435                 440                 445             
Asp Phe Trp Gln Tyr His Asp Gly Cys Gly Ser Trp Val Gln Asp Pro 
    450                 455                 460                 
Ala Lys Gln Ile Ser Val Glu Asp His Lys Val Ser Leu Glu Pro Tyr 
465                 470                 475                 480 
Arg Arg Glu Ser Arg Lys Ala Leu Lys Ile Phe Lys Ser Ala Cys Asp 
                485                 490                 495     
Leu Val Glu Arg Ala Ser Ile Asp Glu Val Phe Leu Asp Leu Gly Arg 
            500                 505                 510         
Ile Cys Phe Asn Met Leu Met Phe Asp Asn Glu Tyr Glu Leu Thr Gly 
        515                 520                 525             
Asp Leu Lys Leu Lys Asp Ala Leu Ser Asn Ile Arg Glu Ala Phe Ile 
    530                 535                 540                 
Gly Gly Asn Tyr Asp Ile Asn Ser His Leu Pro Leu Ile Pro Glu Lys 
545                 550                 555                 560 
Ile Lys Ser Leu Lys Phe Glu Gly Asp Val Phe Asn Pro Glu Gly Arg 
                565                 570                 575     
Asp Leu Ile Thr Asp Trp Asp Asp Val Ile Leu Ala Leu Gly Ser Gln 
            580                 585                 590         
Val Cys Lys Gly Ile Arg Asp Ser Ile Lys Asp Ile Leu Gly Tyr Thr 
        595                 600                 605             
Thr Ser Cys Gly Leu Ser Ser Thr Lys Asn Val Cys Lys Leu Ala Ser 
    610                 615                 620                 
Asn Tyr Lys Lys Pro Asp Ala Gln Thr Ile Val Lys Asn Asp Cys Leu 
625                 630                 635                 640 
Leu Asp Phe Leu Asp Cys Gly Lys Phe Glu Ile Thr Ser Phe Trp Thr 
                645                 650                 655     
Leu Gly Gly Val Leu Gly Lys Glu Leu Ile Asp Val Leu Asp Leu Pro 
            660                 665                 670         
His Glu Asn Ser Ile Lys His Ile Arg Glu Thr Trp Pro Asp Asn Ala 
        675                 680                 685             
Gly Gln Leu Lys Glu Phe Leu Asp Ala Lys Val Lys Gln Ser Asp Tyr 
    690                 695                 700                 
Asp Arg Ser Thr Ser Asn Ile Asp Pro Leu Lys Thr Ala Asp Leu Ala 
705                 710                 715                 720 
Glu Lys Leu Phe Lys Leu Ser Arg Gly Arg Tyr Gly Leu Pro Leu Ser 
                725                 730                 735     
Ser Arg Pro Val Val Lys Ser Met Met Ser Asn Lys Asn Leu Arg Gly 
            740                 745                 750         
Lys Ser Cys Asn Ser Ile Val Asp Cys Ile Ser Trp Leu Glu Val Phe 
        755                 760                 765             
Cys Ala Glu Leu Thr Ser Arg Ile Gln Asp Leu Glu Gln Glu Tyr Asn 
    770                 775                 780                 
Lys Ile Val Ile Pro Arg Thr Val Ser Ile Ser Leu Lys Thr Lys Ser 
785                 790                 795                 800 
Tyr Glu Val Tyr Arg Lys Ser Gly Pro Val Ala Tyr Lys Gly Ile Asn 
                805                 810                 815     
Phe Gln Ser His Glu Leu Leu Lys Val Gly Ile Lys Phe Val Thr Asp 
            820                 825                 830         
Leu Asp Ile Lys Gly Lys Asn Lys Ser Tyr Tyr Pro Leu Thr Lys Leu 
        835                 840                 845             
Ser Met Thr Ile Thr Asn Phe Asp Ile Ile Asp Leu Gln Lys Thr Val 
    850                 855                 860                 
Val Asp Met Phe Gly Asn Gln Val His Thr Phe Lys Ser Ser Ala Gly 
865                 870                 875                 880 
Lys Glu Asp Glu Glu Lys Thr Thr Ser Ser Lys Ala Asp Glu Lys Thr 
                885                 890                 895     
Pro Lys Leu Glu Cys Cys Lys Tyr Gln Val Thr Phe Thr Asp Gln Lys 
            900                 905                 910         
Ala Leu Gln Glu His Ala Asp Tyr His Leu Ala Leu Lys Leu Ser Glu 
        915                 920                 925             
Gly Leu Asn Gly Ala Glu Glu Ser Ser Lys Asn Leu Ser Phe Gly Glu 
    930                 935                 940                 
Lys Arg Leu Leu Phe Ser Arg Lys Arg Pro Asn Ser Gln His Thr Ala 
945                 950                 955                 960 
Thr Pro Gln Lys Lys Gln Val Thr Ser Ser Lys Asn Ile Leu Ser Phe 
                965                 970                 975     
Phe Thr Arg Lys Lys 
            980     

<210> 3
<211> 68
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo 1 from Example 2

<400> 3
cccaaaccca attaatgtac tgcagaattc agctcgaagc ttggccggat ccagcgtggg     60

actgagtc                                                              68


<210> 4
<211> 73
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo 2

<400> 4
gtctcgtgtc tgtaaaaacg tacgtagatg ccatttctaa aaaaacagac acgagacgac     60

tcagtcccac gct                                                        73


<210> 5
<211> 141
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo 1+2

<400> 5
cccaaaccca attaatgtac tgcagaattc agctcgaagc ttggccggat ccagcgtggg     60

actgagtcgt ctcgtgtctg taaaaacgta cgtagatgcc atttctaaaa aaacagacac    120

gagacgactc agtcccacgc t                                              141


<210> 6
<211> 48
<212> DNA
<213> Artificial Sequence


<220> 
<223> Oligo 3

<400> 6
ccggccaagc ttcgagctga attctgcagt acattaattg ggtttggg                  48


