<110>    SK bioscience Co., Ltd.

<120>    SARS-CoV-2 pseudovirus and manufacturing method thereof

<130>    PCT21-115

<150>    KR 10-2020-0142573
<151>    2020-10-29

<160>    8

<170>    KoPatentIn 3.0

<210>    1
<211>    1251
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    The S protein of the  B.1.1.7 variant with the 19tail
         deletion-D614G mutation


<400>    1
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
  1               5                  10                  15 

Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
             20                  25                  30 

Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
         35                  40                  45 

His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
     50                  55                  60 

Phe His Ala Ile Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp Asn Pro
 65                  70                  75                  80 

Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu Lys Ser
                 85                  90                  95 

Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser Lys Thr
            100                 105                 110 

Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile Lys Val
        115                 120                 125 

Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr His Lys
    130                 135                 140 

Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr Ser Ser Ala
145                 150                 155                 160 

Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp Leu
                165                 170                 175 

Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe Lys
            180                 185                 190 

Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile Asn
        195                 200                 205 

Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu Val
    210                 215                 220 

Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu Ala
225                 230                 235                 240 

Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr
                245                 250                 255 

Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe
            260                 265                 270 

Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys
        275                 280                 285 

Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr
    290                 295                 300 

Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr
305                 310                 315                 320 

Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly
                325                 330                 335 

Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg
            340                 345                 350 

Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser
        355                 360                 365 

Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu
    370                 375                 380 

Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg
385                 390                 395                 400 

Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala
                405                 410                 415 

Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala
            420                 425                 430 

Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr
        435                 440                 445 

Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp
    450                 455                 460 

Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val
465                 470                 475                 480 

Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro
                485                 490                 495 

Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe
            500                 505                 510 

Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr
        515                 520                 525 

Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr
    530                 535                 540 

Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln
545                 550                 555                 560 

Gln Phe Gly Arg Asp Ile Asp Asp Thr Thr Asp Ala Val Arg Asp Pro
                565                 570                 575 

Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val
            580                 585                 590 

Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu
        595                 600                 605 

Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp
    610                 615                 620 

Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe
625                 630                 635                 640 

Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser
                645                 650                 655 

Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln
            660                 665                 670 

Thr Gln Thr Asn Ser His Arg Arg Ala Arg Ser Val Ala Ser Gln Ser
        675                 680                 685 

Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala Tyr
    690                 695                 700 

Ser Asn Asn Ser Ile Ala Ile Pro Ile Asn Phe Thr Ile Ser Val Thr
705                 710                 715                 720 

Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr
                725                 730                 735 

Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln
            740                 745                 750 

Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala
        755                 760                 765 

Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln
    770                 775                 780 

Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser
785                 790                 795                 800 

Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu
                805                 810                 815 

Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys
            820                 825                 830 

Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys
        835                 840                 845 

Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp
    850                 855                 860 

Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr
865                 870                 875                 880 

Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala
                885                 890                 895 

Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val
            900                 905                 910 

Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile
        915                 920                 925 

Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys
    930                 935                 940 

Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val
945                 950                 955                 960 

Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp
                965                 970                 975 

Ile Leu Ala Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg
            980                 985                 990 

Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln
        995                1000                1005 

Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr
   1010                1015                1020 

Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys
1025               1030                1035                1040 

Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly
               1045                1050                1055 

Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe
           1060                1065                1070 

Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg
       1075                1080                1085 

Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg
   1090                1095                1100 

Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr His Asn Thr Phe Val Ser
1105               1110                1115                1120 

Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp
               1125                1130                1135 

Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr
           1140                1145                1150 

Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly
       1155                1160                1165 

Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn
   1170                1175                1180 

Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu
1185               1190                1195                1200 

Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu Gly
               1205                1210                1215 

Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met Leu Cys
           1220                1225                1230 

Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly
       1235                1240                1245 

Ser Cys Cys
   1250    


<210>    2
<211>    1251
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    The S protein of the B.1.351 variant with the 19tail
         deletion-D614G mutation


<400>    2
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
  1               5                  10                  15 

Asn Phe Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
             20                  25                  30 

Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
         35                  40                  45 

His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
     50                  55                  60 

Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Ala
 65                  70                  75                  80 

Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
                 85                  90                  95 

Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
            100                 105                 110 

Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
        115                 120                 125 

Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val Tyr
    130                 135                 140 

Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145                 150                 155                 160 

Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
                165                 170                 175 

Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe
            180                 185                 190 

Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
        195                 200                 205 

Pro Ile Asn Leu Val Arg Gly Leu Pro Gln Gly Phe Ser Ala Leu Glu
    210                 215                 220 

Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225                 230                 235                 240 

Leu His Ile Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp Thr
                245                 250                 255 

Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr Phe
            260                 265                 270 

Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp Cys
        275                 280                 285 

Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe Thr
    290                 295                 300 

Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro Thr
305                 310                 315                 320 

Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly
                325                 330                 335 

Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg
            340                 345                 350 

Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser
        355                 360                 365 

Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu
    370                 375                 380 

Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg
385                 390                 395                 400 

Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Asn Ile Ala
                405                 410                 415 

Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala
            420                 425                 430 

Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr
        435                 440                 445 

Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp
    450                 455                 460 

Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val
465                 470                 475                 480 

Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro
                485                 490                 495 

Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe
            500                 505                 510 

Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr
        515                 520                 525 

Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu Thr
    530                 535                 540 

Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe Gln
545                 550                 555                 560 

Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp Pro
                565                 570                 575 

Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly Val
            580                 585                 590 

Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val Leu
        595                 600                 605 

Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala Asp
    610                 615                 620 

Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val Phe
625                 630                 635                 640 

Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn Ser
                645                 650                 655 

Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr Gln
            660                 665                 670 

Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala Ser Gln Ser
        675                 680                 685 

Ile Ile Ala Tyr Thr Met Ser Leu Gly Val Glu Asn Ser Val Ala Tyr
    690                 695                 700 

Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val Thr
705                 710                 715                 720 

Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys Thr
                725                 730                 735 

Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu Gln
            740                 745                 750 

Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile Ala
        755                 760                 765 

Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys Gln
    770                 775                 780 

Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe Ser
785                 790                 795                 800 

Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile Glu
                805                 810                 815 

Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile Lys
            820                 825                 830 

Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile Cys
        835                 840                 845 

Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr Asp
    850                 855                 860 

Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile Thr
865                 870                 875                 880 

Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe Ala
                885                 890                 895 

Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn Val
            900                 905                 910 

Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala Ile
        915                 920                 925 

Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly Lys
    930                 935                 940 

Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu Val
945                 950                 955                 960 

Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn Asp
                965                 970                 975 

Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp Arg
            980                 985                 990 

Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln Gln
        995                1000                1005 

Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala Thr
   1010                1015                1020 

Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe Cys
1025               1030                1035                1040 

Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His Gly
               1045                1050                1055 

Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn Phe
           1060                1065                1070 

Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro Arg
       1075                1080                1085 

Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln Arg
   1090                1095                1100 

Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val Ser
1105               1110                1115                1120 

Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr Asp
               1125                1130                1135 

Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys Tyr
           1140                1145                1150 

Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser Gly
       1155                1160                1165 

Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu Asn
   1170                1175                1180 

Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu Leu
1185               1190                1195                1200 

Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu Gly
               1205                1210                1215 

Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met Leu Cys
           1220                1225                1230 

Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys Gly
       1235                1240                1245 

Ser Cys Cys
   1250    


<210>    3
<211>    1254
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    The S protein of the P.1 variant with the 19tail deletion-D614G
         mutation


<400>    3
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
  1               5                  10                  15 

Asn Phe Thr Asn Arg Thr Gln Leu Pro Ser Ala Tyr Thr Asn Ser Phe
             20                  25                  30 

Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
         35                  40                  45 

His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
     50                  55                  60 

Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
 65                  70                  75                  80 

Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
                 85                  90                  95 

Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
            100                 105                 110 

Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
        115                 120                 125 

Lys Val Cys Glu Phe Gln Phe Cys Asn Tyr Pro Phe Leu Gly Val Tyr
    130                 135                 140 

Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val Tyr
145                 150                 155                 160 

Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu
                165                 170                 175 

Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Ser Glu Phe
            180                 185                 190 

Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr
        195                 200                 205 

Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu
    210                 215                 220 

Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr
225                 230                 235                 240 

Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser
                245                 250                 255 

Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro
            260                 265                 270 

Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala
        275                 280                 285 

Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys
    290                 295                 300 

Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val
305                 310                 315                 320 

Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys
                325                 330                 335 

Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala
            340                 345                 350 

Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu
        355                 360                 365 

Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro
    370                 375                 380 

Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe
385                 390                 395                 400 

Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly
                405                 410                 415 

Thr Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys
            420                 425                 430 

Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn
        435                 440                 445 

Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe
    450                 455                 460 

Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys
465                 470                 475                 480 

Asn Gly Val Lys Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly
                485                 490                 495 

Phe Gln Pro Thr Tyr Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val
            500                 505                 510 

Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys
        515                 520                 525 

Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn
    530                 535                 540 

Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu
545                 550                 555                 560 

Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val
                565                 570                 575 

Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe
            580                 585                 590 

Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val
        595                 600                 605 

Ala Val Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile
    610                 615                 620 

His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser
625                 630                 635                 640 

Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu Tyr Val
                645                 650                 655 

Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala
            660                 665                 670 

Ser Tyr Gln Thr Gln Thr Asn Ser Pro Arg Arg Ala Arg Ser Val Ala
        675                 680                 685 

Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser
    690                 695                 700 

Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile
705                 710                 715                 720 

Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val
                725                 730                 735 

Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu
            740                 745                 750 

Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr
        755                 760                 765 

Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln
    770                 775                 780 

Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe
785                 790                 795                 800 

Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser
                805                 810                 815 

Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly
            820                 825                 830 

Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp
        835                 840                 845 

Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu
    850                 855                 860 

Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly
865                 870                 875                 880 

Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile
                885                 890                 895 

Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr
            900                 905                 910 

Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn
        915                 920                 925 

Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala
    930                 935                 940 

Leu Gly Lys Leu Gln Asp Val Val Asn Gln Asn Ala Gln Ala Leu Asn
945                 950                 955                 960 

Thr Leu Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val
                965                 970                 975 

Leu Asn Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln
            980                 985                 990 

Ile Asp Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val
        995                1000                1005 

Thr Gln Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu
   1010                1015                1020 

Ala Ala Ile Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val
1025               1030                1035                1040 

Asp Phe Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala
               1045                1050                1055 

Pro His Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu
           1060                1065                1070 

Lys Asn Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His
       1075                1080                1085 

Phe Pro Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val
   1090                1095                1100 

Thr Gln Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr
1105               1110                1115                1120 

Phe Val Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr
               1125                1130                1135 

Val Tyr Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu
           1140                1145                1150 

Asp Lys Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp
       1155                1160                1165 

Ile Ser Gly Ile Asn Ala Ser Phe Val Asn Ile Gln Lys Glu Ile Asp
   1170                1175                1180 

Arg Leu Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu
1185               1190                1195                1200 

Gln Glu Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile
               1205                1210                1215 

Trp Leu Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile
           1220                1225                1230 

Met Leu Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys
       1235                1240                1245 

Ser Cys Gly Ser Cys Cys
   1250                


<210>    4
<211>    1252
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    The S protein of the B.1.617.2 variant with the 19tail
         deletion-D614G mutation


<400>    4
Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Cys Val
  1               5                  10                  15 

Asn Leu Arg Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser Phe
             20                  25                  30 

Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val Leu
         35                  40                  45 

His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr Trp
     50                  55                  60 

Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe Asp
 65                  70                  75                  80 

Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr Glu
                 85                  90                  95 

Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp Ser
            100                 105                 110 

Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val Ile
        115                 120                 125 

Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Asp Val Tyr
    130                 135                 140 

Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Gly Val Tyr Ser Ser
145                 150                 155                 160 

Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe Leu Met Asp
                165                 170                 175 

Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu Phe Val Phe
            180                 185                 190 

Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His Thr Pro Ile
        195                 200                 205 

Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu Glu Pro Leu
    210                 215                 220 

Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln Thr Leu Leu
225                 230                 235                 240 

Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser Ser Gly Trp
                245                 250                 255 

Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln Pro Arg Thr
            260                 265                 270 

Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp Ala Val Asp
        275                 280                 285 

Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu Lys Ser Phe
    290                 295                 300 

Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg Val Gln Pro
305                 310                 315                 320 

Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe
                325                 330                 335 

Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn
            340                 345                 350 

Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn
        355                 360                 365 

Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys
    370                 375                 380 

Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe Val Ile
385                 390                 395                 400 

Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile
                405                 410                 415 

Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys Val Ile
            420                 425                 430 

Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn
        435                 440                 445 

Tyr Arg Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg
    450                 455                 460 

Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Lys Pro Cys Asn Gly
465                 470                 475                 480 

Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln
                485                 490                 495 

Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val Leu Ser
            500                 505                 510 

Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys Lys Ser
        515                 520                 525 

Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe Asn Gly Leu
    530                 535                 540 

Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe Leu Pro Phe
545                 550                 555                 560 

Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala Val Arg Asp
                565                 570                 575 

Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser Phe Gly Gly
            580                 585                 590 

Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln Val Ala Val
        595                 600                 605 

Leu Tyr Gln Gly Val Asn Cys Thr Glu Val Pro Val Ala Ile His Ala
    610                 615                 620 

Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly Ser Asn Val
625                 630                 635                 640 

Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His Val Asn Asn
                645                 650                 655 

Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys Ala Ser Tyr
            660                 665                 670 

Gln Thr Gln Thr Asn Ser Arg Arg Arg Ala Arg Ser Val Ala Ser Gln
        675                 680                 685 

Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn Ser Val Ala
    690                 695                 700 

Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr Ile Ser Val
705                 710                 715                 720 

Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser Val Asp Cys
                725                 730                 735 

Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn Leu Leu Leu
            740                 745                 750 

Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu Thr Gly Ile
        755                 760                 765 

Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala Gln Val Lys
    770                 775                 780 

Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly Phe Asn Phe
785                 790                 795                 800 

Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg Ser Phe Ile
                805                 810                 815 

Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala Gly Phe Ile
            820                 825                 830 

Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg Asp Leu Ile
        835                 840                 845 

Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro Leu Leu Thr
    850                 855                 860 

Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala Gly Thr Ile
865                 870                 875                 880 

Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln Ile Pro Phe
                885                 890                 895 

Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val Thr Gln Asn
            900                 905                 910 

Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe Asn Ser Ala
        915                 920                 925 

Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser Ala Leu Gly
    930                 935                 940 

Lys Leu Gln Asn Val Val Asn Gln Asn Ala Gln Ala Leu Asn Thr Leu
945                 950                 955                 960 

Val Lys Gln Leu Ser Ser Asn Phe Gly Ala Ile Ser Ser Val Leu Asn
                965                 970                 975 

Asp Ile Leu Ser Arg Leu Asp Lys Val Glu Ala Glu Val Gln Ile Asp
            980                 985                 990 

Arg Leu Ile Thr Gly Arg Leu Gln Ser Leu Gln Thr Tyr Val Thr Gln
        995                1000                1005 

Gln Leu Ile Arg Ala Ala Glu Ile Arg Ala Ser Ala Asn Leu Ala Ala
   1010                1015                1020 

Thr Lys Met Ser Glu Cys Val Leu Gly Gln Ser Lys Arg Val Asp Phe
1025               1030                1035                1040 

Cys Gly Lys Gly Tyr His Leu Met Ser Phe Pro Gln Ser Ala Pro His
               1045                1050                1055 

Gly Val Val Phe Leu His Val Thr Tyr Val Pro Ala Gln Glu Lys Asn
           1060                1065                1070 

Phe Thr Thr Ala Pro Ala Ile Cys His Asp Gly Lys Ala His Phe Pro
       1075                1080                1085 

Arg Glu Gly Val Phe Val Ser Asn Gly Thr His Trp Phe Val Thr Gln
   1090                1095                1100 

Arg Asn Phe Tyr Glu Pro Gln Ile Ile Thr Thr Asp Asn Thr Phe Val
1105               1110                1115                1120 

Ser Gly Asn Cys Asp Val Val Ile Gly Ile Val Asn Asn Thr Val Tyr
               1125                1130                1135 

Asp Pro Leu Gln Pro Glu Leu Asp Ser Phe Lys Glu Glu Leu Asp Lys
           1140                1145                1150 

Tyr Phe Lys Asn His Thr Ser Pro Asp Val Asp Leu Gly Asp Ile Ser
       1155                1160                1165 

Gly Ile Asn Ala Ser Val Val Asn Ile Gln Lys Glu Ile Asp Arg Leu
   1170                1175                1180 

Asn Glu Val Ala Lys Asn Leu Asn Glu Ser Leu Ile Asp Leu Gln Glu
1185               1190                1195                1200 

Leu Gly Lys Tyr Glu Gln Tyr Ile Lys Trp Pro Trp Tyr Ile Trp Leu
               1205                1210                1215 

Gly Phe Ile Ala Gly Leu Ile Ala Ile Val Met Val Thr Ile Met Leu
           1220                1225                1230 

Cys Cys Met Thr Ser Cys Cys Ser Cys Leu Lys Gly Cys Cys Ser Cys
       1235                1240                1245 

Gly Ser Cys Cys
   1250        


<210>    5
<211>    3756
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleic acids encoding the S protein of the B.1.1.7 variant with
         the 19tail deletion-D614G mutation


<400>    5
atgttcgtgt ttctggtcct gctgcctctg gtgtcttcac agtgcgtcaa tctgactacc         60

cggactcagc tgcctcccgc ttataccaat agcttcaccc ggggcgtgta ctatccagat        120

aaggtgttta gaagctccgt gctgcactcc acacaggacc tgtttctgcc cttctttagc        180

aacgtgacct ggttccacgc catctccggc accaatggca caaagcggtt cgataatccc        240

gtgctgcctt ttaacgacgg cgtgtacttc gcctccaccg agaagtctaa catcatcaga        300

ggctggatct ttggcaccac actggatagc aagacacagt ccctgctgat cgtgaacaat        360

gccaccaacg tggtcatcaa ggtgtgcgag ttccagtttt gtaatgaccc tttcctgggc        420

gtgtatcaca agaacaataa gtcttggatg gagagcgagt ttagagtgta ctctagcgcc        480

aacaattgca catttgagta cgtgagccag ccattcctga tggatctgga gggcaagcag        540

ggcaatttca agaacctgag ggagttcgtg tttaagaata tcgacggcta cttcaaaatc        600

tactccaagc acacccccat caacctggtg cgcgatctgc ctcagggctt ctctgccctg        660

gagccactgg tggacctgcc tatcggcatc aacatcaccc ggtttcagac actgctggcc        720

ctgcacagaa gctacctgac acctggcgat tcctctagcg gatggaccgc aggagctgcc        780

gcctactatg tgggctacct gcagccaagg accttcctgc tgaagtataa cgagaatggc        840

accatcacag acgccgtgga ttgcgccctg gacccactgt ctgagacaaa gtgtacactg        900

aagagcttta ccgtggagaa gggcatctac cagacatcta atttcagggt gcagcccacc        960

gagagcatcg tgcgctttcc taatatcaca aacctgtgcc catttggcga ggtgttcaac       1020

gccacccgct tcgccagcgt gtatgcctgg aataggaagc gcatctccaa ctgcgtggcc       1080

gattattctg tgctgtacaa ctccgcctct ttcagcacct ttaagtgtta cggcgtgagc       1140

cccacaaagc tgaatgacct gtgctttacc aacgtgtatg ccgactcctt cgtgatcagg       1200

ggcgatgagg tgcgccagat cgcacctgga cagacaggca agatcgccga ttacaattat       1260

aagctgccag acgatttcac cggctgcgtg atcgcctgga actctaacaa tctggacagc       1320

aaagtgggcg gcaactacaa ttatctgtac cggctgttta gaaagtccaa cctgaagccc       1380

ttcgagaggg acatctccac agaaatctac caggccggct ctaccccttg caatggcgtg       1440

gagggcttta actgttattt cccactgcag agctacggct tccagcccac ctacggcgtg       1500

ggctatcagc cttaccgcgt ggtggtgctg tcttttgagc tgctgcacgc accagcaaca       1560

gtgtgcggac ccaagaagag caccaatctg gtgaagaaca agtgcgtgaa cttcaacttc       1620

aacggcctga ccggcacagg cgtgctgacc gagtccaaca agaagttcct gccctttcag       1680

cagttcggca gggacatcga cgataccaca gacgccgtgc gcgatccaca gaccctggag       1740

atcctggaca tcacaccctg ctccttcggc ggcgtgtctg tgatcacacc tggcaccaat       1800

acaagcaacc aggtggccgt gctgtaccag ggcgtgaatt gtaccgaggt gcctgtggcc       1860

atccacgccg atcagctgac cccaacatgg cgggtgtata gcaccggctc caacgtgttc       1920

cagacaagag ccggatgtct gatcggagca gagcacgtga acaatagcta cgagtgcgac       1980

atccccatcg gcgccggcat ctgtgcctcc tatcagaccc agacaaactc ccacaggaga       2040

gcccggtctg tggcctccca gtctatcatc gcctacacaa tgagcctggg cgccgagaac       2100

agcgtggcct attctaacaa tagcatcgcc atcccaatca acttcaccat ctccgtgacc       2160

acagagatcc tgcccgtgag catgaccaag acatccgtgg attgcacaat gtacatctgt       2220

ggcgactcca ccgagtgctc taacctgctg ctgcagtatg gcagcttttg tacccagctg       2280

aatagagccc tgacaggcat cgccgtggag caggacaaga acacacagga ggtgttcgcc       2340

caggtgaagc aaatctacaa gaccccccct atcaaggatt ttggcggctt caatttttcc       2400

cagatcctgc ctgacccatc caagccttct aagcggagct ttatcgagga tctgctgttc       2460

aacaaggtga ccctggccga cgccggcttc atcaagcagt acggcgactg cctgggcgac       2520

atcgcagccc gggacctgat ctgcgcccag aagtttaatg gcctgaccgt gctgccaccc       2580

ctgctgacag acgagatgat cgcccagtat acatccgccc tgctggccgg caccatcaca       2640

tctggatgga ccttcggcgc aggagccgcc ctgcagatcc cctttgccat gcagatggcc       2700

tacaggttca acggcatcgg cgtgacccag aatgtgctgt atgagaacca gaagctgatc       2760

gccaatcagt ttaacagcgc catcggcaag atccaggatt ctctgtcctc tacagccagc       2820

gccctgggca agctgcagga cgtggtgaat cagaacgccc aggccctgaa taccctggtg       2880

aagcagctga gcagcaactt cggcgccatc tctagcgtgc tgaatgacat cctggcccgg       2940

ctggataagg tggaggccga ggtgcagatc gaccggctga tcacaggcag actgcagtcc       3000

ctgcagacct acgtgacaca gcagctgatc agggcagcag agatcagggc ctctgccaat       3060

ctggccgcca ccaagatgag cgagtgcgtg ctgggccagt ccaagagagt ggatttttgt       3120

ggcaagggct accacctgat gagcttccct cagtccgccc cacacggcgt ggtgtttctg       3180

cacgtgacct atgtgcccgc ccaggagaag aacttcacca cagcacctgc catctgccac       3240

gacggcaagg cacactttcc cagggagggc gtgttcgtgt ctaacggcac ccactggttt       3300

gtgacacagc gcaatttcta cgagcctcag atcatcacca cacacaatac atttgtgagc       3360

ggcaactgtg acgtggtcat cggcatcgtg aacaataccg tgtacgaccc actgcagccc       3420

gagctggatt cttttaagga ggagctggac aagtatttca agaatcacac cagcccagac       3480

gtggatctgg gcgacatctc tggcatcaat gccagcgtgg tgaacatcca gaaggagatc       3540

gatcgcctga acgaggtggc caagaatctg aacgagagcc tgatcgacct gcaggagctg       3600

ggcaagtatg agcagtacat caagtggccc tggtacatct ggctgggctt catcgccggc       3660

ctgatcgcca tcgtgatggt gaccatcatg ctgtgctgta tgacttcctg ttgctcctgt       3720

ctgaaaggct gttgttcctg tgggtcctgc tgctaa                                 3756


<210>    6
<211>    3756
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleic acids encoding the S protein of the B.1.351 variant with
         the 19tail deletion-D614G mutation


<400>    6
atgtttgtct tcctggtcct gctgcctctg gtctcttctc agtgcgtcaa ctttactacc         60

cgaactcagc tgccccctgc ctacaccaat tccttcacca ggggcgtgta ctatcctgac        120

aaggtgtttc gcagctccgt gctgcactcc acacaggatc tgtttctgcc attcttttct        180

aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgcc        240

aatcccgtgc tgccttttaa cgacggcgtg tacttcgcct ccaccgagaa gtctaacatc        300

atcagaggct ggatctttgg caccacactg gatagcaaga cacagtccct gctgatcgtg        360

aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgaccccttc        420

ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tagggtgtac        480

tctagcgcca acaattgcac atttgagtac gtgagccagc ctttcctgat ggacctggag        540

ggcaagcagg gcaatttcaa gaacctgcgc gagttcgtgt ttaagaatat cgatggctac        600

ttcaaaatct actccaagca cacccccatc aacctggtga ggggcctgcc tcagggcttc        660

tctgccctgg agccactggt ggacctgcct atcggcatca acatcacccg ctttcagaca        720

ctgcacatca gctacctgac accaggcgat tcctctagcg gatggaccgc aggagctgcc        780

gcctactatg tgggctacct gcagcctagg accttcctgc tgaagtataa cgagaatggc        840

accatcacag acgcagtgga ttgcgccctg gaccccctgt ctgagacaaa gtgtacactg        900

aagagcttta ccgtggagaa gggcatctac cagacaagca atttccgggt gcagccaacc        960

gagtccatcg tgagatttcc taatatcaca aacctgtgcc catttggcga ggtgttcaac       1020

gccacccgct tcgccagcgt gtatgcctgg aataggaagc gcatctccaa ctgcgtggcc       1080

gactattctg tgctgtacaa ctccgcctct ttcagcacct ttaagtgcta cggcgtgagc       1140

cccacaaagc tgaatgacct gtgctttacc aacgtgtatg ccgattcctt cgtgatcagg       1200

ggcgacgagg tgcgccagat cgcacctgga cagacaggca atatcgccga ctacaactat       1260

aagctgccag acgatttcac cggctgcgtg atcgcctgga acagcaacaa tctggatagc       1320

aaagtgggcg gcaactacaa ttatctgtac cggctgttta gaaagtctaa cctgaagccc       1380

ttcgagaggg acatctccac agaaatctac caggccggct ctaccccttg caatggcgtg       1440

aagggcttta actgttattt cccactgcag agctacggct tccagcccac ctacggcgtg       1500

ggctatcagc cttaccgcgt ggtggtgctg tcttttgagc tgctgcacgc accagcaaca       1560

gtgtgcggac ccaagaagag caccaatctg gtgaagaaca agtgcgtgaa cttcaacttc       1620

aacggcctga ccggcacagg cgtgctgacc gagtccaaca agaagttcct gccatttcag       1680

cagttcggcc gggacatcgc agataccaca gacgccgtgc gggaccccca gaccctggag       1740

atcctggaca tcacaccctg ctccttcggc ggcgtgtctg tgatcacacc cggcaccaat       1800

acaagcaacc aggtggccgt gctgtaccag ggcgtgaatt gtaccgaggt gcctgtggca       1860

atccacgcag accagctgac cccaacatgg agggtgtata gcaccggctc caacgtgttc       1920

cagacacgcg ccggatgtct gatcggagca gagcacgtga acaatagcta cgagtgcgac       1980

atcccaatcg gcgccggcat ctgtgcctcc tatcagaccc agacaaactc cccaaggaga       2040

gcccggtctg tggcctccca gtctatcatc gcctacacca tgagcctggg cgtggagaac       2100

agcgtggcct attctaacaa tagcatcgcc atcccaacca acttcacaat ctccgtgacc       2160

acagagatcc tgcccgtgag catgaccaag acatccgtgg actgcacaat gtacatctgt       2220

ggcgattcca ccgagtgctc taacctgctg ctgcagtatg gctctttttg tacccagctg       2280

aatagagccc tgacaggcat cgccgtggag caggacaaga acacacagga ggtgttcgcc       2340

caggtgaagc aaatctacaa gaccccccct atcaaggact ttggcggctt caatttttcc       2400

cagatcctgc ctgatccatc caagccttct aagcggagct ttatcgagga cctgctgttc       2460

aacaaggtga ccctggccga tgccggcttc atcaagcagt acggcgattg cctgggcgac       2520

atcgcagccc gggacctgat ctgcgcccag aagtttaatg gcctgaccgt gctgccaccc       2580

ctgctgacag atgagatgat cgcccagtat acatctgccc tgctggccgg caccatcaca       2640

agcggatgga ccttcggcgc aggagccgcc ctgcagatcc cctttgccat gcagatggcc       2700

tacaggttca acggcatcgg cgtgacccag aatgtgctgt atgagaacca gaagctgatc       2760

gccaatcagt ttaactccgc catcggcaag atccaggact ctctgtcctc tacagccagc       2820

gccctgggca agctgcagga tgtggtgaat cagaacgccc aggccctgaa taccctggtg       2880

aagcagctga gcagcaactt cggcgccatc tctagcgtgc tgaatgacat cctgagccgg       2940

ctggacaagg tggaggcaga ggtgcagatc gaccggctga tcacaggcag actgcagtcc       3000

ctgcagacct acgtgacaca gcagctgatc agggcagcag agatcagggc ctctgccaat       3060

ctggccgcca ccaagatgag cgagtgcgtg ctgggccagt ccaagagagt ggacttttgt       3120

ggcaagggct accacctgat gagcttccct cagtccgccc cacacggcgt ggtgtttctg       3180

cacgtgacct atgtgcccgc ccaggagaag aacttcacca cagcccctgc catctgccac       3240

gatggcaagg cccactttcc acgggagggc gtgttcgtga gcaacggcac ccactggttt       3300

gtgacacaga gaaatttcta tgagccccag atcatcacca cagacaatac cttcgtgagc       3360

ggcaactgtg acgtggtcat cggaatcgtg aacaataccg tgtacgatcc actgcagccc       3420

gagctggact cttttaagga ggagctggat aagtatttca agaatcacac cagccctgac       3480

gtggatctgg gcgacatctc tggcatcaat gccagcgtgg tgaacatcca gaaggagatc       3540

gaccggctga acgaggtggc caagaatctg aacgagagcc tgatcgatct gcaggagctg       3600

ggcaagtatg agcagtacat caagtggccc tggtacatct ggctgggctt catcgccggc       3660

ctgatcgcca tcgtgatggt gaccatcatg ctgtgctgta tgaccagttg ctgctcctgc       3720

ctgaaagggt gttgttcttg tggctcttgt tgttga                                 3756


<210>    7
<211>    3765
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleic acids encoding the S protein of the P.1 variant with the
         19tail deletion-D614G mutation


<400>    7
atgtttgtct tcctggtcct gctgcccctg gtgtcctctc agtgcgtcaa tttcactaat         60

agaactcagc tgccctccgc ttatacaaat tccttcaccc ggggcgtgta ctatcctgac        120

aaggtgttta gaagctccgt gctgcactcc acacaggatc tgtttctgcc attcttttct        180

aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac        240

aatcccgtgc tgccttttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc        300

atcagaggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg        360

aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa ttatcccttc        420

ctgggcgtgt actatcacaa gaacaataag tcttggatgg agagcgagtt tagggtgtac        480

tctagcgcca acaattgcac atttgagtac gtgagccagc ctttcctgat ggacctggag        540

ggcaagcagg gcaatttcaa gaacctgtcc gagttcgtgt ttaagaatat cgatggctac        600

ttcaaaatct actctaagca cacccccatc aacctggtgc gcgacctgcc tcagggcttc        660

agcgccctgg agccactggt ggatctgcct atcggcatca acatcacccg gtttcagaca        720

ctgctggccc tgcacagatc ctacctgaca cccggcgact cctctagcgg atggaccgca        780

ggagctgccg cctactatgt gggctacctg cagcctagga ccttcctgct gaagtataac        840

gagaatggca ccatcacaga cgcagtggat tgcgccctgg accccctgtc tgagacaaag        900

tgtacactga agagctttac cgtggagaag ggcatctacc agacaagcaa tttcagggtg        960

cagccaaccg agtccatcgt gcgctttcct aatatcacaa acctgtgccc atttggcgag       1020

gtgttcaacg ccacccgctt cgccagcgtg tatgcctgga ataggaagcg catctccaac       1080

tgcgtggccg actattctgt gctgtacaac agcgccagct tcagcacctt caagtgttac       1140

ggcgtgagcc ccacaaagct gaatgacctg tgctttacca acgtgtatgc cgattccttc       1200

gtgatcaggg gcgacgaggt gcgccagatc gcacctggac agaccggcac aatcgcagac       1260

tacaattata agctgccaga cgatttcacc ggctgcgtga tcgcctggaa ctctaacaat       1320

ctggatagca aagtgggcgg caactacaat tatctgtacc ggctgtttag aaagtccaac       1380

ctgaagccct tcgagcggga catctccaca gaaatctacc aggccggctc taccccttgc       1440

aatggcgtga agggctttaa ctgttatttc ccactgcagt cttacggctt ccagcccacc       1500

tacggcgtgg gctatcagcc ttacagagtg gtggtgctgt cttttgagct gctgcacgca       1560

ccagcaacag tgtgcggacc caagaagagc accaatctgg tgaagaacaa gtgcgtgaac       1620

ttcaacttca acggcctgac cggcacaggc gtgctgaccg agtccaacaa gaagttcctg       1680

ccatttcagc agttcggcag ggacatcgca gataccacag acgccgtgcg cgacccacag       1740

accctggaga tcctggacat cacaccctgc tccttcggcg gcgtgtctgt gatcacaccc       1800

ggcaccaata caagcaacca ggtggccgtg ctgtaccagg gcgtgaattg taccgaggtg       1860

cctgtggcaa tccacgcaga ccagctgacc ccaacatggc gggtgtatag caccggctcc       1920

aacgtgttcc agacaagagc cggctgtctg atcggcgccg agtatgtgaa caatagctac       1980

gagtgcgaca tcccaatcgg cgccggcatc tgtgcctcct accagaccca gacaaactcc       2040

ccaaggagag cccggtctgt ggcctcccag tctatcatcg cctacaccat gagcctgggc       2100

gccgagaaca gcgtggccta ttctaacaat agcatcgcca tcccaaccaa cttcacaatc       2160

tccgtgacca cagagatcct gcccgtgagc atgaccaaga catccgtgga ctgcacaatg       2220

tacatctgtg gcgattccac cgagtgctct aacctgctgc tgcagtatgg cagcttttgt       2280

acccagctga atagagccct gacaggcatc gccgtggagc aggacaagaa cacacaggag       2340

gtgttcgccc aggtgaagca aatctacaag acccccccta tcaaggactt tggcggcttc       2400

aacttcagcc agatcctgcc tgatccatcc aagccttcta agaggagctt tatcgaggac       2460

ctgctgttca acaaggtgac cctggccgat gccggcttca tcaagcagta cggcgattgc       2520

ctgggcgaca tcgcagcccg cgacctgatc tgtgcccaga agtttaatgg cctgaccgtg       2580

ctgccacccc tgctgacaga tgagatgatc gcccagtata catctgccct gctggccggc       2640

accatcacaa gcggatggac cttcggcgca ggagccgccc tgcagatccc ctttgccatg       2700

cagatggcct acaggttcaa cggcatcggc gtgacccaga atgtgctgta tgagaaccag       2760

aagctgatcg ccaatcagtt taactccgcc atcggcaaga tccaggactc tctgtcctct       2820

acagccagcg ccctgggcaa gctgcaggat gtggtgaatc agaacgccca ggccctgaat       2880

accctggtga agcagctgag cagcaacttc ggcgccatct ctagcgtgct gaatgacatc       2940

ctgagccggc tggacaaggt ggaggcagag gtgcagatcg accggctgat caccggcaga       3000

ctgcagtccc tgcagaccta cgtgacacag cagctgatca gggcagcaga gatcagggcc       3060

tctgccaatc tggccgccat caagatgagc gagtgcgtgc tgggccagtc caagagagtg       3120

gacttttgtg gcaagggcta ccacctgatg agcttccctc agtccgcccc acacggcgtg       3180

gtgtttctgc acgtgaccta tgtgcccgcc caggagaaga acttcaccac agcccctgcc       3240

atctgccacg atggcaaggc ccactttcca agggagggcg tgttcgtgtc taacggcacc       3300

cactggtttg tgacacagcg caatttctac gagccccaga tcatcaccac agacaataca       3360

ttcgtgagcg gcaactgtga cgtggtcatc ggcatcgtga acaataccgt gtacgatcca       3420

ctgcagcccg agctggactc ttttaaggag gagctggata agtatttcaa gaatcacacc       3480

agccctgacg tggatctggg cgacatctct ggcatcaatg ccagcttcgt gaacatccag       3540

aaggagatcg accggctgaa cgaggtggcc aagaatctga acgagagcct gatcgatctg       3600

caggagctgg gcaagtatga gcagtacatc aagtggccct ggtacatctg gctgggcttt       3660

atcgccggcc tgatcgccat cgtgatggtg accatcatgc tgtgctgtat gacctcttgc       3720

tgttcctgcc tgaagggctg ctgtagttgt ggctcctgtt gctga                       3765


<210>    8
<211>    3759
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Nucleic acids encoding the S protein of the B.1.617.2 variant
         with the 19tail deletion-D614G mutation


<400>    8
atgtttgtct tcctggtcct gctgcctctg gtctcatctc agtgcgtcaa cctgcgaacc         60

cgaactcagc tgccacccgc ttacaccaat tccttcacac gcggcgtgta ctatccagac        120

aaggtgttcc ggagcagcgt gctgcactcc acacaggatc tgtttctgcc cttcttttct        180

aacgtgacct ggttccacgc catccacgtg agcggcacca atggcacaaa gcggttcgac        240

aatcccgtgc tgccttttaa cgatggcgtg tacttcgcct ccaccgagaa gtctaacatc        300

atccggggct ggatctttgg caccacactg gacagcaaga cacagtccct gctgatcgtg        360

aacaatgcca ccaacgtggt catcaaggtg tgcgagttcc agttttgtaa tgatcctttc        420

ctggacgtgt actatcacaa gaacaataag tcttggatgg agagcggcgt gtattctagc        480

gccaacaatt gcacatttga gtacgtgtcc cagccattcc tgatggacct ggagggcaag        540

cagggcaatt tcaagaacct gagagagttc gtgtttaaga atatcgatgg ctacttcaaa        600

atctactcca agcacacccc catcaacctg gtgagggacc tgcctcaggg cttctctgcc        660

ctggagcccc tggtggatct gcctatcggc atcaacatca ccagatttca gacactgctg        720

gccctgcaca ggagctacct gacacctggc gactcctcta gcggatggac cgcaggagct        780

gccgcctact atgtgggcta tctgcagcca cgcaccttcc tgctgaagta caacgagaat        840

ggcaccatca cagacgcagt ggattgcgcc ctggaccccc tgtctgagac aaagtgtaca        900

ctgaagagct ttaccgtgga gaagggcatc tatcagacaa gcaatttccg cgtgcagcca        960

accgagtcca tcgtgcggtt tcctaatatc acaaacctgt gcccatttgg cgaggtgttc       1020

aacgcaacca ggttcgccag cgtgtacgca tggaatcgca agcggatctc caactgcgtg       1080

gccgactatt ctgtgctgta caactccgcc tctttcagca cctttaagtg ctatggcgtg       1140

agccccacaa agctgaatga cctgtgcttt accaacgtgt acgccgattc cttcgtgatc       1200

aggggcgacg aggtgcggca gatcgcacct ggacagacag gcaagatcgc cgactacaat       1260

tataagctgc cagacgattt caccggctgc gtgatcgcct ggaactctaa caatctggat       1320

agcaaagtgg gcggcaacta caattataga tacaggctgt ttagaaagtc taatctgaag       1380

cccttcgaga gggacatctc cacagaaatc taccaggccg gctctaagcc ttgcaatggc       1440

gtggagggct ttaactgtta tttcccactg cagagctacg gcttccagcc caccaacggc       1500

gtgggctatc agccttacag ggtggtggtg ctgtcttttg agctgctgca cgcaccagca       1560

acagtgtgcg gacccaagaa gagcaccaat ctggtgaaga acaagtgcgt gaacttcaac       1620

ttcaacggcc tgaccggcac aggcgtgctg accgagtcca acaagaagtt cctgcccttt       1680

cagcagttcg gcagagacat cgccgatacc acagacgccg tgagggaccc acagaccctg       1740

gagatcctgg acatcacacc ctgctccttc ggcggcgtgt ctgtgatcac acctggcacc       1800

aatacaagca accaggtggc cgtgctgtat cagggcgtga attgtaccga ggtgcctgtg       1860

gcaatccacg cagaccagct gaccccaaca tggcgcgtgt acagcaccgg ctccaacgtg       1920

tttcagacac gggccggatg tctgatcgga gcagagcacg tgaacaatag ctatgagtgc       1980

gacatcccca tcggcgccgg catctgtgcc tcctaccaga cccagaccaa cagccggcgg       2040

cgggcccgga gcgtggcctc ccagtctatc atcgcctata ccatgagcct gggcgccgag       2100

aacagcgtgg cctactctaa caatagcatc gccatcccaa ccaacttcac aatctccgtg       2160

accacagaga tcctgcccgt gagcatgacc aagacatccg tggactgcac aatgtatatc       2220

tgtggcgatt ccaccgagtg ctctaacctg ctgctgcagt acggctcttt ttgtacccag       2280

ctgaataggg ccctgacagg aatcgcagtg gagcaggaca agaacacaca ggaggtgttc       2340

gcccaggtga agcaaatcta caagaccccc cctatcaagg actttggcgg cttcaatttt       2400

tcccagatcc tgcctgatcc atccaagcct tctaagcgca gctttatcga ggacctgctg       2460

ttcaacaagg tgaccctggc cgatgccggc ttcatcaagc agtatggcga ttgcctgggc       2520

gacatcgcag cccgggacct gatctgtgcc cagaagttta acggcctgac cgtgctgcca       2580

ccactgctga cagatgagat gatcgcccag tacacatctg ccctgctggc cggcaccatc       2640

acaagcggat ggaccttcgg cgcaggagcc gccctgcaga tcccctttgc catgcagatg       2700

gcctatcgct tcaatggcat cggcgtgacc cagaatgtgc tgtacgagaa ccagaagctg       2760

atcgccaatc agtttaactc cgccatcggc aagatccagg actctctgtc ctctacagcc       2820

agcgccctgg gcaagctgca gaacgtggtg aatcagaacg cccaggccct gaataccctg       2880

gtgaagcagc tgagcagcaa cttcggcgcc atctctagcg tgctgaatga catcctgagc       2940

cggctggaca aggtggaggc agaggtgcag atcgatagac tgatcacagg caggctgcag       3000

tccctgcaga cctatgtgac acagcagctg atcagggcag cagagatcag ggcctctgcc       3060

aatctggccg ccaccaagat gagcgagtgc gtgctgggcc agtccaagag ggtggacttt       3120

tgtggcaagg gctatcacct gatgagcttc cctcagtccg ccccacacgg cgtggtgttt       3180

ctgcacgtga cctacgtgcc cgcccaggag aagaacttca ccacagcccc tgccatctgc       3240

cacgatggca aggcccactt tcccagagag ggcgtgttcg tgagcaacgg cacccactgg       3300

tttgtgacac agaggaattt ctacgagcct cagatcatca ccacagacaa taccttcgtg       3360

agcggcaact gtgacgtggt catcggcatc gtgaacaata ccgtgtatga tccactgcag       3420

cccgagctgg actcttttaa ggaggagctg gataagtact tcaagaatca caccagccca       3480

gacgtggatc tgggcgacat ctctggcatc aatgccagcg tggtgaacat ccagaaggag       3540

atcgaccggc tgaacgaggt ggccaagaat ctgaacgaga gcctgatcga tctgcaggag       3600

ctgggcaagt atgagcagta catcaagtgg ccctggtaca tctggctggg cttcatcgcc       3660

ggcctgatcg ccatcgtgat ggtgaccatc atgctgtgct gtatgacctc ctgctgttct       3720

tgcctgaagg gatgttgctc ctgtggctct tgttgctga                              3759
