<110>    CJ CheilJedang Corporation

<120>    Microorganism with reduced viscous production ability and
         fermented soy product obtained by the same

<130>    OPP20214139KR

<150>    KR 10-2020-0142298
<151>    2020-10-29

<160>    11

<170>    koPatentIn 3.0

<210>    1
<211>    428
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    ObgE (wild type)


<400>    1
Met Phe Val Asp Gln Val Lys Val Tyr Val Lys Gly Gly Asp Gly Gly
  1               5                  10                  15 

Asn Gly Met Val Ala Phe Arg Arg Glu Lys Tyr Val Pro Lys Gly Gly
             20                  25                  30 

Pro Ala Gly Gly Asp Gly Gly Lys Gly Gly Asp Val Val Phe Glu Val
         35                  40                  45 

Asp Glu Gly Leu Arg Thr Leu Met Asp Phe Arg Tyr Lys Lys His Phe
     50                  55                  60 

Lys Ala Ile Arg Gly Glu His Gly Met Ser Lys Asn Gln His Gly Arg
 65                  70                  75                  80 

Asn Ala Asp Asp Met Val Ile Lys Val Pro Pro Gly Thr Val Val Thr
                 85                  90                  95 

Asp Asp Asp Thr Lys Gln Val Ile Ala Asp Leu Thr Glu His Gly Gln
            100                 105                 110 

Arg Ala Val Ile Ala Arg Gly Gly Arg Gly Gly Arg Gly Asn Ser Arg
        115                 120                 125 

Phe Ala Thr Pro Ala Asn Pro Ala Pro Gln Leu Ser Glu Asn Gly Glu
    130                 135                 140 

Pro Gly Lys Glu Arg Tyr Ile Val Leu Glu Leu Lys Val Leu Ala Asp
145                 150                 155                 160 

Val Gly Leu Val Gly Phe Pro Ser Val Gly Lys Ser Thr Leu Leu Ser
                165                 170                 175 

Val Val Ser Ser Ala Lys Pro Lys Ile Ala Asp Tyr His Phe Thr Thr
            180                 185                 190 

Leu Val Pro Asn Leu Gly Met Val Glu Thr Asp Asp Gly Arg Ser Phe
        195                 200                 205 

Val Met Ala Asp Leu Pro Gly Leu Ile Glu Gly Ala His Gln Gly Val
    210                 215                 220 

Gly Leu Gly His Gln Phe Leu Arg His Ile Glu Arg Thr Arg Val Ile
225                 230                 235                 240 

Val His Val Ile Asp Met Ser Gly Leu Glu Gly Arg Asp Pro Tyr Glu
                245                 250                 255 

Asp Tyr Leu Thr Ile Asn Gln Glu Leu Ser Glu Tyr Asn Leu Arg Leu
            260                 265                 270 

Thr Glu Arg Pro Gln Ile Ile Val Ala Asn Lys Met Asp Met Pro Glu
        275                 280                 285 

Ala Ala Glu Asn Leu Glu Ala Phe Lys Glu Lys Leu Thr Asp Asp Tyr
    290                 295                 300 

Pro Val Phe Pro Ile Ser Ala Val Thr Arg Glu Gly Leu Arg Glu Leu
305                 310                 315                 320 

Leu Phe Glu Val Ala Asn Gln Leu Glu Asn Thr Pro Glu Phe Pro Leu
                325                 330                 335 

Tyr Asp Glu Glu Glu Leu Thr Gln Asn Arg Val Met Tyr Thr Met Glu
            340                 345                 350 

Asn Glu Glu Val Pro Phe Asn Ile Thr Arg Asp Pro Asp Gly Val Phe
        355                 360                 365 

Val Leu Ser Gly Asp Ser Leu Glu Arg Leu Phe Lys Met Thr Asp Phe
    370                 375                 380 

Ser Arg Asp Glu Ser Val Lys Arg Phe Ala Arg Gln Met Arg Gly Met
385                 390                 395                 400 

Gly Val Asp Glu Ala Leu Arg Glu Arg Gly Ala Lys Asp Gly Asp Ile
                405                 410                 415 

Ile Arg Leu Leu Glu Phe Glu Phe Glu Phe Ile Asp
            420                 425            


<210>    2
<211>    1287
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    ObgE gene encoding ObgE (wild type)


<400>    2
atgtttgtag atcaggtcaa agtatatgta aaaggcggcg acggcggcaa cggtatggtt         60

gcgtttcgcc gtgaaaaata tgtgccgaaa ggcggccctg ccggcggtga cggaggaaag        120

ggaggagacg tcgtttttga agtagatgaa ggtctccgca ccctgatgga ttttagatac        180

aaaaaacact ttaaagcgat tcgcggcgag catggcatgt ccaaaaacca gcacgggcga        240

aatgctgatg atatggtcat taaagttccg ccgggcaccg ttgtgacaga cgatgataca        300

aaacaggtca tcgctgattt aacagagcac ggacagcggg ctgtcattgc aagaggcgga        360

agaggaggaa gagggaatag ccgctttgct acaccggcca atcccgcgcc tcagctttca        420

gaaaacggcg agccgggaaa agaacgctac attgttcttg aattaaaagt gcttgcagat        480

gtcggacttg tcgggtttcc aagtgtggga aaatctactt tgctgtctgt tgtctcatct        540

gcaaaaccga aaattgcgga ctatcacttt acaacgcttg tcccgaatct cggcatggtt        600

gaaacggatg acggacgcag ctttgtcatg gctgatttgc cggggctgat tgaaggcgca        660

caccaaggcg tcggacttgg ccaccagttt ttgcgccata ttgaacggac gagggttatt        720

gttcacgtca ttgacatgtc aggcttagaa ggccgcgatc cgtatgagga ttatcttacg        780

attaaccagg agctgagcga gtacaatctg cgtctcactg agcgtccgca aattatcgtt        840

gcaaataaaa tggacatgcc ggaggctgcg gaaaatctcg aagcctttaa agaaaaactg        900

acggatgatt atccggtatt cccgatcagt gcggtgacaa gagaaggtct gcgtgagctt        960

ctgtttgagg ttgccaatca gcttgagaac acaccggaat tcccgctgta tgacgaggaa       1020

gagcttacac aaaaccgtgt catgtacacg atggaaaatg aagaagtgcc atttaacatt       1080

acacgcgatc cagacggtgt atttgtgctt tcaggagaca gccttgagcg gttattcaag       1140

atgactgatt tctcacgtga tgaatcggtt aaacggtttg caagacagat gcgcggaatg       1200

ggtgttgatg aagcactcag agaacgcgga gccaaggatg gagatataat caggcttctg       1260

gaatttgaat ttgaatttat tgattaa                                           1287


<210>    3
<211>    428
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    ObgE (D83V variant)


<400>    3
Met Phe Val Asp Gln Val Lys Val Tyr Val Lys Gly Gly Asp Gly Gly
  1               5                  10                  15 

Asn Gly Met Val Ala Phe Arg Arg Glu Lys Tyr Val Pro Lys Gly Gly
             20                  25                  30 

Pro Ala Gly Gly Asp Gly Gly Lys Gly Gly Asp Val Val Phe Glu Val
         35                  40                  45 

Asp Glu Gly Leu Arg Thr Leu Met Asp Phe Arg Tyr Lys Lys His Phe
     50                  55                  60 

Lys Ala Ile Arg Gly Glu His Gly Met Ser Lys Asn Gln His Gly Arg
 65                  70                  75                  80 

Asn Ala Val Asp Met Val Ile Lys Val Pro Pro Gly Thr Val Val Thr
                 85                  90                  95 

Asp Asp Asp Thr Lys Gln Val Ile Ala Asp Leu Thr Glu His Gly Gln
            100                 105                 110 

Arg Ala Val Ile Ala Arg Gly Gly Arg Gly Gly Arg Gly Asn Ser Arg
        115                 120                 125 

Phe Ala Thr Pro Ala Asn Pro Ala Pro Gln Leu Ser Glu Asn Gly Glu
    130                 135                 140 

Pro Gly Lys Glu Arg Tyr Ile Val Leu Glu Leu Lys Val Leu Ala Asp
145                 150                 155                 160 

Val Gly Leu Val Gly Phe Pro Ser Val Gly Lys Ser Thr Leu Leu Ser
                165                 170                 175 

Val Val Ser Ser Ala Lys Pro Lys Ile Ala Asp Tyr His Phe Thr Thr
            180                 185                 190 

Leu Val Pro Asn Leu Gly Met Val Glu Thr Asp Asp Gly Arg Ser Phe
        195                 200                 205 

Val Met Ala Asp Leu Pro Gly Leu Ile Glu Gly Ala His Gln Gly Val
    210                 215                 220 

Gly Leu Gly His Gln Phe Leu Arg His Ile Glu Arg Thr Arg Val Ile
225                 230                 235                 240 

Val His Val Ile Asp Met Ser Gly Leu Glu Gly Arg Asp Pro Tyr Glu
                245                 250                 255 

Asp Tyr Leu Thr Ile Asn Gln Glu Leu Ser Glu Tyr Asn Leu Arg Leu
            260                 265                 270 

Thr Glu Arg Pro Gln Ile Ile Val Ala Asn Lys Met Asp Met Pro Glu
        275                 280                 285 

Ala Ala Glu Asn Leu Glu Ala Phe Lys Glu Lys Leu Thr Asp Asp Tyr
    290                 295                 300 

Pro Val Phe Pro Ile Ser Ala Val Thr Arg Glu Gly Leu Arg Glu Leu
305                 310                 315                 320 

Leu Phe Glu Val Ala Asn Gln Leu Glu Asn Thr Pro Glu Phe Pro Leu
                325                 330                 335 

Tyr Asp Glu Glu Glu Leu Thr Gln Asn Arg Val Met Tyr Thr Met Glu
            340                 345                 350 

Asn Glu Glu Val Pro Phe Asn Ile Thr Arg Asp Pro Asp Gly Val Phe
        355                 360                 365 

Val Leu Ser Gly Asp Ser Leu Glu Arg Leu Phe Lys Met Thr Asp Phe
    370                 375                 380 

Ser Arg Asp Glu Ser Val Lys Arg Phe Ala Arg Gln Met Arg Gly Met
385                 390                 395                 400 

Gly Val Asp Glu Ala Leu Arg Glu Arg Gly Ala Lys Asp Gly Asp Ile
                405                 410                 415 

Ile Arg Leu Leu Glu Phe Glu Phe Glu Phe Ile Asp
            420                 425            


<210>    4
<211>    1287
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    ObgE gene (A248T) encoding ObgE (D83V variant)


<400>    4
atgtttgtag atcaggtcaa agtatatgta aaaggcggcg acggcggcaa cggtatggtt         60

gcgtttcgcc gtgaaaaata tgtgccgaaa ggcggccctg ccggcggtga cggaggaaag        120

ggaggagacg tcgtttttga agtagatgaa ggtctccgca ccctgatgga ttttagatac        180

aaaaaacact ttaaagcgat tcgcggcgag catggcatgt ccaaaaacca gcacgggcga        240

aatgctgttg atatggtcat taaagttccg ccgggcaccg ttgtgacaga cgatgataca        300

aaacaggtca tcgctgattt aacagagcac ggacagcggg ctgtcattgc aagaggcgga        360

agaggaggaa gagggaatag ccgctttgct acaccggcca atcccgcgcc tcagctttca        420

gaaaacggcg agccgggaaa agaacgctac attgttcttg aattaaaagt gcttgcagat        480

gtcggacttg tcgggtttcc aagtgtggga aaatctactt tgctgtctgt tgtctcatct        540

gcaaaaccga aaattgcgga ctatcacttt acaacgcttg tcccgaatct cggcatggtt        600

gaaacggatg acggacgcag ctttgtcatg gctgatttgc cggggctgat tgaaggcgca        660

caccaaggcg tcggacttgg ccaccagttt ttgcgccata ttgaacggac gagggttatt        720

gttcacgtca ttgacatgtc aggcttagaa ggccgcgatc cgtatgagga ttatcttacg        780

attaaccagg agctgagcga gtacaatctg cgtctcactg agcgtccgca aattatcgtt        840

gcaaataaaa tggacatgcc ggaggctgcg gaaaatctcg aagcctttaa agaaaaactg        900

acggatgatt atccggtatt cccgatcagt gcggtgacaa gagaaggtct gcgtgagctt        960

ctgtttgagg ttgccaatca gcttgagaac acaccggaat tcccgctgta tgacgaggaa       1020

gagcttacac aaaaccgtgt catgtacacg atggaaaatg aagaagtgcc atttaacatt       1080

acacgcgatc cagacggtgt atttgtgctt tcaggagaca gccttgagcg gttattcaag       1140

atgactgatt tctcacgtga tgaatcggtt aaacggtttg caagacagat gcgcggaatg       1200

ggtgttgatg aagcactcag agaacgcgga gccaaggatg gagatataat caggcttctg       1260

gaatttgaat ttgaatttat tgattaa                                           1287


<210>    5
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    obgE_F primer


<400>    5
ccatgattac gccaagctgg atatgttccc caagc                                    35


<210>    6
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    obgE_R primer


<400>    6
ggccgttact agtggatcta accgctcaag gctg                                     34


<210>    7
<211>    1491
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    16S rRNA of C27 strain


<400>    7
ctctcagacg aacgctggcg gcgtgcctaa tacatgcaag tcgagcggac agatgggagc         60

ttgctccctg atgttagcgg cggacgggtg agtaacacgt gggtaacctg cctgtaagac        120

tgggataact ccgggaaacc ggggctaata ccggatggtt gtttgaaccg catggttcaa        180

acataaaagg tggcttcggc taccacttac agatggaccc gcggcgcatt agctagttgg        240

tgaggtaacg gctcaccaag gcgacgatgc gtagccgacc tgagagggtg atcggccaca        300

ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat cttccgcaat        360

ggacgaaagt ctgacggagc aacgccgcgt gagtgatgaa ggttttcgga tcgtaaagct        420

ctgttgttag ggaagaacaa gtaccgttcg aatagggcgg taccttgacg gtacctaacc        480

agaaagccac ggctaactac gtgccagcag ccgcggtaat acgtaggtgg caagcgttgt        540

ccggaattat tgggcgtaaa gggctcgcag gcggtttctt aagtctgatg tgaaagcccc        600

cggctcaacc ggggagggtc attggaaact ggggaacttg agtgcagaag aggagagtgg        660

aattccacgt gtagcggtga aatgcgtaga gatgtggagg aacaccagtg gcgaaggcga        720

ctctctggtc tgtaactgac gctgaggagc gaaagcgtgg ggagcgaaca ggattagata        780

ccctggtagt ccacgccgta aacgatgagt gctaagtgtt agggggtttc cgccccttag        840

tgctgcagct aacgcattaa gcactccgcc tggggagtac ggtcgcaaga ctgaaactca        900

aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg        960

aagaacctta ccaggtcttg acatcctctg acaatcctag agataggacg tccccttcgg       1020

gggcagagtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa       1080

gtcccgcaac gagcgcaacc cttgatctta gttgccagca ttcagttggg cactctaagg       1140

tgactgccgg tgacaaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat       1200

gacctgggct acacacgtgc tacaatggac agaacaaagg gcagcgaaac cgcgaggtta       1260

agccaatccc acaaatctgt tctcagttcg gatcgcagtc tgcaactcga ctgcgtgaag       1320

ctggaatcgc tagtaatcgc ggatcagcat gccgcggtga atacgttccc gggccttgta       1380

cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taacctttta       1440

ggagccagcc gccgaaggtg ggacagatga ttggggtgaa gtcgtcaaag g                1491


<210>    8
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    27F_primer


<400>    8
agagtttgat cmtggctcag                                                     20


<210>    9
<211>    22
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    1492R_primer


<400>    9
tacggytacc ttgttacgac tt                                                  22


<210>    10
<211>    18
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    785F_primer


<400>    10
ggattagata ccctggta                                                       18


<210>    11
<211>    20
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    907R_primer


<400>    11
ccgtcaattc mtttragttt                                                     20
