               SEQUENCE LISTING

<110> CENTRE SCIENTIFIQUE DE MONACO, INSTITUT NATIONAL DE LA SANTE ET DE LA RECHERCHE MEDICALE, 
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE COTE D'AZUR

<120> NEW SPLICE VARIANT ISOFORM OF VEGF

<130> B380299PCT_D41798
<150> EP 20204454.1
<151> 2020-10-28

<160> 95

<170> BiSSAP 1.3.6

<210> 1
<211> 248
<212> PRT
<213> Homo sapiens


<220> 
<223> VEGF222/NF (Protein sequence)

<400> 1
Met Asn Phe Leu Leu Ser Trp Val His Trp Ser Leu Ala Leu Leu Leu 
1               5                   10                  15      
Tyr Leu His His Ala Lys Trp Ser Gln Ala Ala Pro Met Ala Glu Gly 
            20                  25                  30          
Gly Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln 
        35                  40                  45              
Arg Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu 
    50                  55                  60                  
Tyr Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu 
65                  70                  75                  80  
Met Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro 
                85                  90                  95      
Thr Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His 
            100                 105                 110         
Gln Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys 
        115                 120                 125             
Glu Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro Cys Gly 
    130                 135                 140                 
Pro Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr 
145                 150                 155                 160 
Cys Lys Cys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln 
                165                 170                 175     
Leu Glu Leu Asn Glu Arg Thr Cys Ser Leu Cys Phe Pro Phe Pro Ser 
            180                 185                 190         
Asp Val Thr Ser Arg Gly Gly Glu Pro Gly Arg Arg Lys Glu Pro Pro 
        195                 200                 205             
Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly Lys Thr Asp Thr Glu 
    210                 215                 220                 
Arg Ser Ile Gln Lys Pro Arg Cys Arg His His Thr Ile Thr Ile Asp 
225                 230                 235                 240 
Arg Thr Val Leu Asn Pro Glu Thr 
                245             

<210> 2
<211> 263
<212> PRT
<213> Artificial Sequence


<220> 
<223> SlP-HIs-3C-VEGF222/NF

<400> 2
Met Asn Phe Tyr Lys Ile Phe Val Phe Val Ala Leu Ile Leu Ala Ile 
1               5                   10                  15      
Ser Ile Gly Gln Ser Glu Ala Gly Ser His His His His His His Ser 
            20                  25                  30          
Gly Leu Glu Val Leu Phe Gln Gly Pro Ala Pro Met Ala Glu Gly Gly 
        35                  40                  45              
Gly Gln Asn His His Glu Val Val Lys Phe Met Asp Val Tyr Gln Arg 
    50                  55                  60                  
Ser Tyr Cys His Pro Ile Glu Thr Leu Val Asp Ile Phe Gln Glu Tyr 
65                  70                  75                  80  
Pro Asp Glu Ile Glu Tyr Ile Phe Lys Pro Ser Cys Val Pro Leu Met 
                85                  90                  95      
Arg Cys Gly Gly Cys Cys Asn Asp Glu Gly Leu Glu Cys Val Pro Thr 
            100                 105                 110         
Glu Glu Ser Asn Ile Thr Met Gln Ile Met Arg Ile Lys Pro His Gln 
        115                 120                 125             
Gly Gln His Ile Gly Glu Met Ser Phe Leu Gln His Asn Lys Cys Glu 
    130                 135                 140                 
Cys Arg Pro Lys Lys Asp Arg Ala Arg Gln Glu Asn Pro Cys Gly Pro 
145                 150                 155                 160 
Cys Ser Glu Arg Arg Lys His Leu Phe Val Gln Asp Pro Gln Thr Cys 
                165                 170                 175     
Lys Cys Ser Cys Lys Asn Thr Asp Ser Arg Cys Lys Ala Arg Gln Leu 
            180                 185                 190         
Glu Leu Asn Glu Arg Thr Cys Ser Leu Cys Phe Pro Phe Pro Ser Asp 
        195                 200                 205             
Val Thr Ser Arg Gly Gly Glu Pro Gly Arg Arg Lys Glu Pro Pro Ser 
    210                 215                 220                 
Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly Lys Thr Asp Thr Glu Arg 
225                 230                 235                 240 
Ser Ile Gln Lys Pro Arg Cys Arg His His Thr Ile Thr Ile Asp Arg 
                245                 250                 255     
Thr Val Leu Asn Pro Glu Thr 
            260             

<210> 3
<211> 747
<212> DNA
<213> Homo sapiens


<220> 
<223> VEGF222/NF (cDNA sequence)

<400> 3
atgaactttc tgctgtcttg ggtgcattgg agccttgcct tgctgctcta cctccaccat      60

gccaagtggt cccaggctgc acccatggca gaaggaggag ggcagaatca tcacgaagtg     120

gtgaagttca tggatgtcta tcagcgcagc tactgccatc caatcgagac cctggtggac     180

atcttccagg agtaccctga tgagatcgag tacatcttca agccatcctg tgtgcccctg     240

atgcgatgcg ggggctgctg caatgacgag ggcctggagt gtgtgcccac tgaggagtcc     300

aacatcacca tgcagattat gcggatcaaa cctcaccaag gccagcacat aggagagatg     360

agcttcctac agcacaacaa atgtgaatgc agaccaaaga aagatagagc aagacaagaa     420

aatccctgtg ggccttgctc agagcggaga aagcatttgt ttgtacaaga tccgcagacg     480

tgtaaatgtt cctgcaaaaa cacagactcg cgttgcaagg cgaggcagct tgagttaaac     540

gaacgtactt gcagcctttg ttttccattt ccctcagatg tgacaagccg aggcggtgag     600

ccgggcagga ggaaggagcc tccctcaggg tttcgggaac cagatctctc accaggaaag     660

actgatacag aacgatcgat acagaaacca cgctgccgcc accacaccat caccatcgac     720

agaacagtcc ttaatccaga aacctga                                         747


<210> 4
<211> 747
<212> RNA
<213> Homo sapiens


<220> 
<223> VEGF222/NF (RNA sequence)

<400> 4
augaacuuuc ugcugucuug ggugcauugg agccuugccu ugcugcucua ccuccaccau      60

gccaaguggu cccaggcugc acccauggca gaaggaggag ggcagaauca ucacgaagug     120

gugaaguuca uggaugucua ucagcgcagc uacugccauc caaucgagac ccugguggac     180

aucuuccagg aguacccuga ugagaucgag uacaucuuca agccauccug ugugccccug     240

augcgaugcg ggggcugcug caaugacgag ggccuggagu gugugcccac ugaggagucc     300

aacaucacca ugcagauuau gcggaucaaa ccucaccaag gccagcacau aggagagaug     360

agcuuccuac agcacaacaa augugaaugc agaccaaaga aagauagagc aagacaagaa     420

aaucccugug ggccuugcuc agagcggaga aagcauuugu uuguacaaga uccgcagacg     480

uguaaauguu ccugcaaaaa cacagacucg cguugcaagg cgaggcagcu ugaguuaaac     540

gaacguacuu gcagccuuug uuuuccauuu cccucagaug ugacaagccg aggcggugag     600

ccgggcagga ggaaggagcc ucccucaggg uuucgggaac cagaucucuc accaggaaag     660

acugauacag aacgaucgau acagaaacca cgcugccgcc accacaccau caccaucgac     720

agaacagucc uuaauccaga aaccuga                                         747


<210> 5
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> Epitope#1 of the VEGF222/NF

<400> 5
Asp Val Thr Ser Arg Gly Gly Glu Pro Gly Arg Arg Lys Glu 
1               5                   10                  

<210> 6
<211> 14
<212> PRT
<213> Artificial Sequence


<220> 
<223> Epitope#2 of the VEGF222/NF

<400> 6
Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly Lys Thr Asp 
1               5                   10                  

<210> 7
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer

<400> 7
gcctttgttt tccatttccc t                                              21


<210> 8
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer

<400> 8
tctgtcgatg gtgatggtgt g                                              21


<210> 9
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> CDKPRR

<400> 9
Cys Asp Lys Pro Arg Arg 
1               5       

<210> 10
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> SLTRKD

<400> 10
Ser Leu Thr Arg Lys Asp 
1               5       

<210> 11
<211> 62
<212> RNA
<213> Artificial Sequence


<220> 
<223> VEGF pre-mRNA

<400> 11
gccucaucgc caggccuccu cacuuggccc uaaccccagc cuuuguuuuc cauuucccuc     60

ag                                                                    62


<210> 12
<211> 63
<212> PRT
<213> Artificial Sequence


<220> 
<223> resulting C-terminal specific sequence of VEGF222/NF

<400> 12
Leu Cys Phe Pro Phe Pro Ser Asp Val Thr Ser Arg Gly Gly Glu Pro 
1               5                   10                  15      
Gly Arg Arg Lys Glu Pro Pro Ser Gly Phe Arg Glu Pro Asp Leu Ser 
            20                  25                  30          
Pro Gly Lys Thr Asp Thr Glu Arg Ser Ile Gln Lys Pro Arg Cys Arg 
        35                  40                  45              
His His Thr Ile Thr Ile Asp Arg Thr Val Leu Asn Pro Glu Thr 
    50                  55                  60              

<210> 13
<211> 7
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 1 of VEGF222/NF of Homo Sapiens (HS), Gorilla
      Gorilla (GG) and Pan Troglodytes (PT)

<400> 13
Ser Leu Cys Phe Pro Phe Pro 
1               5           

<210> 14
<211> 57
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 of VEGF222/NF of Homo Sapiens (HS) and Pan
      Troglodytes (PT)

<400> 14
Ser Asp Val Thr Ser Arg Gly Gly Glu Pro Gly Arg Arg Lys Glu Pro 
1               5                   10                  15      
Pro Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly Lys Thr Asp Thr 
            20                  25                  30          
Glu Arg Ser Ile Gln Lys Pro Arg Cys Arg His His Thr Ile Thr Ile 
        35                  40                  45              
Asp Arg Thr Val Leu Asn Pro Glu Thr 
    50                  55          

<210> 15
<211> 36
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 of VEGF222/NF of Gorilla Gorilla (GG)

<400> 15
Ser Asp Val Thr Ser Arg Gly Gly Glu Pro Gly Arg Arg Lys Glu Pro 
1               5                   10                  15      
Pro Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly Lys Thr Asp Thr 
            20                  25                  30          
Glu Arg Ser Ile 
        35      

<210> 16
<211> 16
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 1 of VEGF222/NF of Sus Scrofa (SS)

<400> 16
Ala Ser Ser Pro Gly Pro Asn Pro Arg Pro Pro Phe Pro Val Pro Arg 
1               5                   10                  15      



<210> 17
<211> 50
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 of VEGF222/NF of Sus Scrofa (SS)

<400> 17
Ala Asp Val Thr Ser Arg Gly Gly Glu Pro Gly Trp Arg Lys Glu Pro 
1               5                   10                  15      
Pro Ser Ala Phe Trp Ala Pro Asp Val Ser Pro Gly Lys Thr Asp Thr 
            20                  25                  30          
Glu Arg Pro Val Ala Ala Ala Thr Thr Thr Pro Pro Pro Ser Thr Glu 
        35                  40                  45              
Gln Ser 
    50  

<210> 18
<211> 56
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment of VEGF222/NF of Canis Lupus (CL)

<400> 18
Trp Gly Gly Cys Leu Pro His His Cys Val Pro Val Pro Gly Pro Asn 
1               5                   10                  15      
Asn Pro Trp Pro Leu Ile Pro Thr Asp Val Thr Ser Pro Gly Gly Glu 
            20                  25                  30          
Pro Gly Trp Lys Lys Glu Pro Pro Ser Gly Phe Arg Glu Pro Asp Leu 
        35                  40                  45              
Ser Pro Gly Lys Pro Asp Ser Glu 
    50                  55      

<210> 19
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 1 of VEGF222/NF of Mus Musculus (MM)

<400> 19
Arg Pro Gly Glu Ala Ala Cys Leu His Cys Gln Ala Pro Val Ala Leu 
1               5                   10                  15      
Thr Pro Cys Leu Ser Leu Pro Phe Pro 
            20                  25  

<210> 20
<211> 52
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 of VEGF222/NF of Mus Musculus (MM)

<400> 20
Ile Asp Val Thr Ser Gln Gly Gly Glu Pro Gly Cys Arg Lys Glu Pro 
1               5                   10                  15      
Pro Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Glu Arg Pro Ile Asn 
            20                  25                  30          
His Val Thr Thr Thr Pro Ser Ser Ser Pro Leu Thr Glu Gln Ser Leu 
        35                  40                  45              
Ile Gln Lys Ala 
    50          

<210> 21
<211> 21
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 1 of VEGF222/NF of Rattus Norvegicus (RN)

<400> 21
Arg Leu Leu Ala Ser Ser Pro Gly Ser Cys Gly Pro Asn Pro Arg Leu 
1               5                   10                  15      
Phe Leu Pro Phe Ala 
            20      

<210> 22
<211> 52
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 of VEGF222/NF of Rattus Norvegicus (RN)

<400> 22
Thr Asp Val Thr Ser Gln Gly Gly Glu Pro Gly Cys Arg Lys Glu Pro 
1               5                   10                  15      
Pro Ser Gly Phe Arg Glu Leu Asp Leu Ser Pro Glu Arg Pro Ile Asn 
            20                  25                  30          
His Val Thr Thr Thr Pro Pro Ser Ser Pro Ser Thr Glu Gln Ser Leu 
        35                  40                  45              
Ile Gln Lys Ala 
    50          

<210> 23
<211> 6
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 1 consensus of VEGF222/NF between species

<400> 23
Leu Cys Phe Pro Phe Pro 
1               5       

<210> 24
<211> 10
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 2 consensus of VEGF222/NF between species

<400> 24
Asp Val Thr Ser Arg Gly Gly Glu Pro Gly 
1               5                   10  

<210> 25
<211> 22
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminal Fragment 3 consensus of VEGF222/NF between species

<400> 25
Arg Lys Glu Pro Pro Ser Gly Phe Arg Glu Pro Asp Leu Ser Pro Gly 
1               5                   10                  15      
Lys Thr Asp Thr Glu Arg 
            20          

<210> 26
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shScramble

<400> 26
cctaaggtta agtcgccctc g                                              21


<210> 27
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shVEGF#1

<400> 27
gcgcaagaaa tcccggtata a                                              21


<210> 28
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shVEGF#2

<400> 28
agggcagaat catcacgaag t                                              21


<210> 29
<211> 19
<212> RNA
<213> Artificial Sequence


<220> 
<223> shVEGFNF#1

<400> 29
gcctttgttt tccatttcc                                                 19


<210> 30
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shVEGFNF#2

<400> 30
catttccctc agatgtgaca a                                              21


<210> 31
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shNRP1

<400> 31
tgtggatgac attagtatta a                                              21


<210> 32
<211> 21
<212> RNA
<213> Artificial Sequence


<220> 
<223> shNRP2

<400> 32
cctcaacttc aaccctcact t                                              21


<210> 33
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward VEGF222/NF = PCR Forward VEGF222/NF

<400> 33
gcctttgttt tccatttccc t                                              21


<210> 34
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse VEGF222/NF = PCR Reverse VEGF222/NF

<400> 34
tctgtcgatg gtgatggtgt g                                              21


<210> 35
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward VEGF = PCR Forward VEGF

<400> 35
tttctgctgt cttgggtgca ttgg                                           24


<210> 36
<211> 24
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse VEGF = PCR Reverse VEGF

<400> 36
accacttcgt gatgattctg ccct                                           24


<210> 37
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward VEGF121

<400> 37
atcttcaagc catcctgtgt gc                                             22


<210> 38
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse VEGF121

<400> 38
tgcgcttgtc acatttttct tg                                             22


<210> 39
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward human 36b4

<400> 39
cagattggct acccaactgt t                                              21


<210> 40
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse human 36b4

<400> 40
ggccaggact cgtttgtacc                                                20


<210> 41
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward mouse 36b4

<400> 41
agattcggga tatgctgttg gc                                             22


<210> 42
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse mouse 36b4

<400> 42
tcgggtccta gaccagtgtt c                                              21


<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward CD31

<400> 43
acgctggtgc tctatgcaag                                                20


<210> 44
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse CD31

<400> 44
tcagttgctg cccattcatc a                                              21


<210> 45
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward VEGFR2

<400> 45
tttggcaaat acaacccttc aga                                            23


<210> 46
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse VEGFR2

<400> 46
gcagaagata ctgtcaccac c                                              21


<210> 47
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward αSMA

<400> 47
gtcccagaca tcagggagta a                                              21


<210> 48
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse αSMA

<400> 48
tcggatactt cagcgtcagg a                                              21


<210> 49
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward CD45

<400> 49
cctcacctgc tcctcaaact tc                                             22


<210> 50
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse CD45

<400> 50
catccacttt gccctctgct tc                                             22


<210> 51
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward IL-1

<400> 51
gcaactgttc ctgaactcaa ct                                             22


<210> 52
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse IL-1

<400> 52
atcttttggg gtccgtcaac t                                              21


<210> 53
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward TNFα

<400> 53
ctatgtcagc ctcttctc                                                  18


<210> 54
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse TNFα

<400> 54
catttgggaa cttctcatcc                                                20


<210> 55
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward iNOS

<400> 55
tcaccttcga gggcagccga                                                20


<210> 56
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse iNOS

<400> 56
tccgtggcaa agcgagccag                                                20


<210> 57
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward TGFβ

<400> 57
atacgcctga gtggctgtct                                                20


<210> 58
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse TGFβ

<400> 58
cgctgaatcg aaagccctgt                                                20


<210> 59
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward ARG1

<400> 59
gattatcgga gcgcctttct                                                20


<210> 60
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse ARG1

<400> 60
ccacactgac tcttccattc tt                                             22


<210> 61
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward PD-1

<400> 61
accctggtca ttcacttggg                                                20


<210> 62
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse PD-1

<400> 62
catttgctcc ctctgacact g                                              21


<210> 63
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward TIM-3

<400> 63
tcaggtctta ccctcaactg tg                                             22


<210> 64
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse TIM-3

<400> 64
ggcattctta ccaacctcaa aca                                            23


<210> 65
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Forward CTLA-4

<400> 65
ttttgtagcc ctgctcactc t                                              21


<210> 66
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> qPCR Reverse CTLA-4

<400> 66
ctgaaggttg ggtcacctgt a                                              21


<210> 67
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> PCR Forward 36b4

<400> 67
ggcgacctgg aagtccaac                                                 19


<210> 68
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> PCR Reverse 36b4

<400> 68
ccatcagcac cacagccttc                                                20


<210> 69
<211> 792
<212> DNA
<213> Artificial Sequence


<220> 
<223> SlP-HIs-3C-VEGF222/NF

<400> 69
atgaacttct acaagatctt cgtcttcgtc gccctgatcc tggccatcag catcggtcag      60

agcgaagctg gtagccacca ccaccaccat cacagcggtc tggaagtgct gttccagggt     120

cctgccccta tggctgaggg cggtggtcag aaccaccacg aagtggtcaa gttcatggac     180

gtgtaccagc gcagctactg ccaccctatc gaaactctgg tggacatctt ccaggagtac     240

cctgacgaga tcgagtacat cttcaagcct agctgcgtgc ctctgatgcg ttgcggtggt     300

tgctgcaacg acgagggtct ggagtgcgtg cctactgaag agagcaacat cactatgcag     360

atcatgcgca tcaagcctca ccagggtcag cacatcggtg aaatgagctt cctgcagcac     420

aacaagtgcg aatgccgccc taagaaggac cgcgctcgcc aggaaaaccc ttgcggtcct     480

tgctccgagc gtcgcaagca cctgttcgtg caggaccccc agacctgcaa gtgctcctgc     540

aagaacactg actcccgttg caaggcccgt cagctggagc tgaacgagcg cacctgcagc     600

ctgtgcttcc ctttccccag cgacgtcact tcccgtggcg gcgagcctgg tcgccgtaag     660

gaacccccta gcggtttccg cgaacccgac ctgtcccccg gtaaaaccga caccgagcgt     720

agcatccaga agcctcgctg ccgtcaccac accatcacca tcgaccgcac cgtgctgaac     780

cctgaaacct aa                                                         792


<210>  70
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward VEGFNF total

<400>  70
cctttgtttt ccatttccct                                                   20


<210>  71
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse VEGFNF total

<400>  71
tctgtcgatg gtgatggtgt g                                                 21


<210>  72
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  PCR Forward VEGFNF total

<400>  72
gcggatcaaa cctcaccaag g                                                 21


<210>  73
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  PCR Reverse VEGFNF total

<400>  73
gcttgtcaca tctgagggaa atg                                               23


<210>  74
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF165

<400>  74
tgtttgtaca agatccgcag acgtg                                             25


<210>  75
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse hVEGF165

<400>  75
ctcggcttgt cacatctgca agtacg                                            26


<210>  76
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 168/NF

<400>  76
cccactgagg agtccaacat cac                                               23


<210>  77
<211>  27
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse  hVEGF 168/NF

<400>  77
ggaaaacaaa ggctgcattc acatttg                                           27


<210>  78
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 178/NF

<400>  78
gcggatcaaa cctcaccaag g                                                 21


<210>  79
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse  hVEGF 178/NF

<400>  79
ggaaaacaaa ggttttcttg tcttgctc                                          28


<210>  80
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 202/NF

<400>  80
gcggatcaaa cctcaccaag g                                                 21


<210>  81
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse  hVEGF 202/NF

<400>  81
ggaaaacaaa ggacgctcca ggacttatac                                        30


<210>  82
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 222/NF

<400>  82
caagacaaga aaatccctgt gggc                                              24


<210>  83
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse hVEGF 222/NF

<400>  83
ggaaatggaa aacaaaggct gcaagtac                                          28


<210>  84
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 240/NF

<400>  84
caagaaatcc cgtccctgtg gg                                                22


<210>  85
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse hVEGF 240/NF

<400>  85
ggaaatggaa aacaaaggct gcaagtac                                          28


<210>  86
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 246/NF

<400>  86
cctggagcgt tccctgtgg                                                    19


<210>  87
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse hVEGF 246/NF

<400>  87
ggaaatggaa aacaaaggct gcaagtac                                          28


<210>  88
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward hVEGF 263/NF

<400>  88
cctggagcgt gtacgttggt g                                                 21


<210>  89
<211>  28
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse hVEGF 263/NF

<400>  89
ggaaatggaa aacaaaggct gcaagtac                                          28


<210>  90
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward mVEGFR3

<400>  90
cgagtcggag ccttctgagg                                                   20


<210>  91
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse  mVEGFR3

<400>  91
gcagtccagc aatagggggt                                                   20


<210>  92
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward mLYVE-1

<400>  92
cagcacacta gcctggtgtt a                                                 21


<210>  93
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse mLYVE-1

<400>  93
cgcccatgat tctgcatgta ga                                                22


<210>  94
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Forward mPROX1

<400>  94
tgcgtgttgc accacagaat a                                                 21


<210>  95
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  qPCR Reverse  mPROX1

<400>  95
agaagggttg acattggagt ga                                                22


