               SEQUENCE LISTING

<110> UNIVERSITAET HAMBURG

<120> TRANSCRIPTIONAL SYNCHRONIZATION OF TWO OR MORE FUNCTIONAL TRANSCRIPTION PRODUCTS

<130> PAT 1793 WO

<150> LU102162
<151> 2020-10-26
<150> DE102020128062.9
<151> 2020-10-26

<160> 23

<170> BiSSAP 1.3.6

<210> 1
<211> 188
<212> RNA
<213> Artificial Sequence


<220> 
<223> Lariat capping ribozyme (LCrz)

<400> 1
gguuggguug ggaaguauca uggcuaauca ccaugaugca aucggguuga acacuuaauu     60

ggguuaaaac ggugggggac gaucccguaa cauccguccu aacggcgaca gacugcacgg    120

cccugccucu uagguguguu caaugaacag ucguuccgaa aggaagcauc cgguauccca    180

agacaauc                                                             188


<210> 2
<211> 68
<212> RNA
<213> Artificial Sequence


<220> 
<223> Hepatitis delta virus ribozyme (HDVRz) var1

<400> 2
ccggccguac cagggucgga ggagcgaccg cggccgaccc guuguacgaa gccguaccgc     60

uuacccug                                                              68


<210> 3
<211> 88
<212> RNA
<213> Artificial Sequence


<220> 
<223> Hepatitis delta virus ribozyme (HDVRz) var2

<400> 3
agggucggca uggcaucucc accuccucgc gguccgaccu gggcuacuuc gguaggcuaa     60

gggagaagcu uggcacuggc cgucguuu                                        88


<210> 4
<211> 67
<212> RNA
<213> Artificial Sequence


<220> 
<223> CPEB3 ribozyme

<400> 4
gggggccaca gcagaagcgu ucacgucgca gccccuguca gauucuggug aaucugcgaa     60

uucugcu                                                               67


<210> 5
<211> 78
<212> RNA
<213> Artificial Sequence


<220> 
<223> CPEB3 ribozyme (RF00622)

<400> 5
aggggauaac agggggccac agcagaagcg uucacgucgc agccccuguc agauucuggu     60

gaaucugcga auucugcu                                                   78


<210> 6
<211> 43
<212> RNA
<213> Artificial Sequence


<220> 
<223> Hammerhead ribozyme (HHRz)

<220> 
<221> misc_feature
<222> 1..6
<223> /note="n = a, g, c, or u"

<400> 6
nnnnnngacu acucaggcac uccugcuuug cucauucgag cag                       43


<210> 7
<211> 53
<212> RNA
<213> Artificial Sequence


<220> 
<223> Hammerhead ribozyme (HHRz)

<220> 
<221> misc_feature
<222> 6..13
<223> /note="n = a, g, c, or u"

<400> 7
gggagnnnnn nnncugauga guccgugagg acgaaacggu acccgguacc guc            53


<210> 8
<211> 64
<212> RNA
<213> Artificial Sequence


<220> 
<223> Type-P5 twister ribozyme

<400> 8
cuuguaaugc ggccguguaa auauuuacac gucggucuca agcccgauaa acgcagagag     60

caag                                                                  64


<210> 9
<211> 76
<212> RNA
<213> Artificial Sequence


<220> 
<223> Twister sister ribozyme

<400> 9
ggacccgcaa ggccgacggc auccgccgcc gcuggugcaa guccagccgc cccggggcgg     60

gcgcucaugg guaaac                                                     76


<210> 10
<211> 81
<212> RNA
<213> Artificial Sequence


<220> 
<223> Twister sister ribozyme

<400> 10
gcaacccgca aggccgacgc acaacgcgcc gccggugcaa gcccggccac ccugcaaggg     60

gugggcgcuc auggguacac a                                               81


<210> 11
<211> 85
<212> RNA
<213> Artificial Sequence


<220> 
<223> Twister sister ribozyme

<400> 11
gaaacccgcu aggccgacag ccucaccgcu gccgcuggug caagcccagc cgccccagac     60

cggggcgggc gcucaugggu aacag                                           85


<210> 12
<211> 81
<212> RNA
<213> Artificial Sequence


<220> 
<223> Twister sister ribozyme

<400> 12
acgacccgca aggccgacgc auaacgcgcc gccggugcaa gcccggccac cccacauggg     60

gcgggcgcuc auggguacac a                                               81


<210> 13
<211> 82
<212> RNA
<213> Artificial Sequence


<220> 
<223> Twister sister ribozyme

<400> 13
augacccgca aggccgacgg caucccgccg ccgcuggugc aagcccagcc gccccgccag     60

ggcgggcgcu caugggucca ca                                              82


<210> 14
<211> 84
<212> RNA
<213> Artificial Sequence


<220> 
<223> Hatchet ribozyme

<400> 14
aaucguucuu acugauauca gugacaaaca uguggggcuu aucuaaucuu cggauuagua     60

uuagugcaga cguuaaaacc augu                                            84


<210> 15
<211> 72
<212> RNA
<213> Artificial Sequence


<220> 
<223> Spacer sequence (long)

<400> 15
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     60

aaaaaaaaaa aa                                                         72


<210> 16
<211> 48
<212> RNA
<213> Artificial Sequence


<220> 
<223> Synthetic Poly-A tail (short)

<400> 16
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa                  48


<210> 17
<211> 300
<212> RNA
<213> Artificial Sequence


<220> 
<223> Synthetic Poly-A tail (long)

<400> 17
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    120

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    180

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    240

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa    300


<210> 18
<211> 4733
<212> DNA
<213> Artificial Sequence


<220> 
<223> Plasmid pEGFP-N1

<400> 18
tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg       60

cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt      120

gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca      180

atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc      240

aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta      300

catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac      360

catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg      420

atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg      480

ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt      540

acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta      600

ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg      660

gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc      720

atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc      780

gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg      840

cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc      900

taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc      960

caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag     1020

ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac     1080

ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg     1140

gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac     1200

ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg     1260

ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag     1320

aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg     1380

gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg     1440

tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa     1500

tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca     1560

atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt     1620

ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc     1680

gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc     1740

ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag     1800

agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc     1860

gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa     1920

gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg     1980

aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt     2040

gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc     2100

gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa     2160

atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat     2220

tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt     2280

gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt     2340

cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc     2400

atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc     2460

cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg     2520

ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc     2580

taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa     2640

gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg     2700

gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc     2760

ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca     2820

gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc     2880

actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca     2940

tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat     3000

acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca     3060

cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg     3120

ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc     3180

gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct     3240

ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct     3300

acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac     3360

ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc     3420

tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag     3480

atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg     3540

ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg     3600

ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa     3660

taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt     3720

cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg     3780

tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca     3840

acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt     3900

taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga     3960

ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca     4020

aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac     4080

caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg     4140

taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag     4200

gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac     4260

cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt     4320

taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg     4380

agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc     4440

ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc     4500

gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc     4560

acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa     4620

acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt     4680

tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat            4733


<210> 19
<211> 366
<212> DNA
<213> Artificial Sequence


<220> 
<223> Synchronization cassette

<220> 
<221> misc_feature
<222> 1..72
<223> /note="synthetic poly A"

<220> 
<221> misc_feature
<222> 73..140
<223> /note="Hepatitis delta virus ribozyme"

<220> 
<221> misc_feature
<222> 141..146
<223> /note="restriction site"

<220> 
<221> misc_feature
<222> 147..194
<223> /note="spacer"

<220> 
<221> misc_feature
<222> 195..200
<223> /note="restriction site"

<220> 
<221> misc_feature
<222> 201..243
<223> /note="Hammerhead ribozyme"

<220> 
<221> misc_feature
<222> 244..298
<223> /note="shThr2"

<220> 
<221> misc_feature
<222> 299..366
<223> /note="Hepatitis delta virus ribozyme"

<400> 19
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa      60

aaaaaaaaaa aaccggccgt accagggtcg gaggagcgac cgcggccgac ccgttgtacg     120

aagccgtacc gcttaccctg tccggaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa     180

aaaaaaaaaa aaaaactagt taccacgact actcaggcac tcctgctttg ctcattcgag     240

caggtggtaa ggcgtcggtc tcgtaattca agagattacg agaccgacgc cttaccaccc     300

ggccgtacca gggtcggagg agcgaccgcg gccgacccgt tgtacgaagc cgtaccgctt     360

accctg                                                                366


<210> 20
<211> 16
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer

<400> 20
ggccaagtgg taaggc                                                    16


<210> 21
<211> 13
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer

<400> 21
aggcacggac ggg                                                       13


<210> 22
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Forward primer

<400> 22
ccataccacc ctgaacgc                                                  18


<210> 23
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> Reverse primer

<400> 23
gtattcccag gcggtctc                                                  18


