                         SEQUENCE LISTING

<110>  Omvax, Inc.
 
<120>  COMPOSITIONS AND METHODS FOR VACCINATION AGAINST NEISSERIA 
       GONORRHOEAE

<130>  OMV0002-401-PC

<150>  63/104,819
<151>  2020-10-23

<160>  56    

<170>  PatentIn version 3.5

<210>  1
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae MetQ protein

<400>  1

Met Lys Thr Phe Phe Lys Thr Leu Ser Ala Ala Ala Leu Ala Leu Ile 
1               5                   10                  15      


Leu Ala Ala Cys Gly Gly Gln Lys Asp Ser Ala Pro Ala Ala Ser Ala 
            20                  25                  30          


Ala Ala Pro Ser Ala Asp Asn Gly Ala Ala Lys Lys Glu Ile Val Phe 
        35                  40                  45              


Gly Thr Thr Val Gly Asp Phe Gly Asp Met Val Lys Glu Gln Ile Gln 
    50                  55                  60                  


Ala Glu Leu Glu Lys Lys Gly Tyr Thr Val Lys Leu Val Glu Phe Thr 
65                  70                  75                  80  


Asp Tyr Val Arg Pro Asn Leu Ala Leu Ala Glu Gly Glu Leu Asp Ile 
                85                  90                  95      


Asn Val Phe Gln His Lys Pro Tyr Leu Asp Asp Phe Lys Lys Glu His 
            100                 105                 110         


Asn Leu Asp Ile Thr Glu Ala Phe Gln Val Pro Thr Ala Pro Leu Gly 
        115                 120                 125             


Leu Tyr Pro Gly Lys Leu Lys Ser Leu Glu Glu Val Lys Asp Gly Ser 
    130                 135                 140                 


Thr Val Ser Ala Pro Asn Asp Pro Ser Asn Phe Ala Arg Ala Leu Val 
145                 150                 155                 160 


Met Leu Asn Glu Leu Gly Trp Ile Lys Leu Lys Asp Gly Ile Asn Pro 
                165                 170                 175     


Leu Thr Ala Ser Lys Ala Asp Ile Ala Glu Asn Leu Lys Asn Ile Lys 
            180                 185                 190         


Ile Val Glu Leu Glu Ala Ala Gln Leu Pro Arg Ser Arg Ala Asp Val 
        195                 200                 205             


Asp Phe Ala Val Val Asn Gly Asn Tyr Ala Ile Ser Ser Gly Met Lys 
    210                 215                 220                 


Leu Thr Glu Ala Leu Phe Gln Glu Pro Ser Phe Ala Tyr Val Asn Trp 
225                 230                 235                 240 


Ser Ala Val Lys Thr Ala Asp Lys Asp Ser Gln Trp Leu Lys Asp Val 
                245                 250                 255     


Thr Glu Ala Tyr Asn Ser Asp Ala Phe Lys Ala Tyr Ala His Lys Arg 
            260                 265                 270         


Phe Glu Gly Tyr Lys Tyr Pro Ala Ala Trp Asn Glu Gly Ala Ala Lys 
        275                 280                 285             


<210>  2
<211>  880
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae MetQ DNA Sequence

<400>  2
atgaaaacct tcttcaaaac cctttccgcc gccgcactcg cgctcatcct cgcagcctgc       60

ggcggtcaaa aagacagcgc gcccgcagcc tctgccgccg ccccttctgc cgataacggc      120

gcggcgaaaa aagaaatcgt cttcggcacg accgtcggcg acttcggcga tatggtcaaa      180

gaacaaatcc aagccgagct ggagaaaaaa ggctacaccg tcaaattggt cgaatttacc      240

gactatgtgc gcccgaatct ggcattggcg gagggcgagt tggacatcaa cgtcttccaa      300

cacaaaccct atcttgacga tttcaaaaaa gaacacaacc tggacatcac cgaagccttc      360

caagtgccga ccgcgccttt gggactgtat ccgggcaaac tgaaatcgct ggaagaagtc      420

aaagacggca gcaccgtatc cgcgcccaac gacccgtcca acttcgcacg cgccttggtg      480

atgctgaacg aactgggttg gatcaaactc aaagacggca tcaatccgct gaccgcatcc      540

aaagccgaca tcgcggaaaa cctgaaaaac atcaaaatcg tcgagcttga agccgcacaa      600

ctgccgcgca gccgcgccga cgtggatttt gccgtcgtca acggcaacta cgccataagc      660

agcggcatga agctgaccga agccctgttc caagagccga gctttgccta tgtcgcctgg      720

tctgccgtca aaaccgccga caaagacagc caatggctta aagacgtaac cgaggcctat      780

aactccgacg cgttcaaagc ctacgcgcac aaacgcttcg agggctacaa ataccctgcc      840

gcatggaatg aaggcgcagc caaataaggc agtcgtataa                            880


<210>  3
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae MetQSM protein

<400>  3

Met Lys Thr Phe Phe Lys Thr Leu Ser Ala Ala Ala Leu Ala Leu Ile 
1               5                   10                  15      


Leu Ala Ala Cys Gly Gly Gln Lys Asp Ser Ala Pro Ala Ala Ser Ala 
            20                  25                  30          


Ala Ala Pro Ser Ala Asp Asn Gly Ala Ala Lys Lys Glu Ile Val Phe 
        35                  40                  45              


Gly Thr Thr Val Gly Asp Phe Gly Asp Met Val Lys Glu Gln Ile Gln 
    50                  55                  60                  


Ala Glu Leu Glu Lys Lys Gly Tyr Thr Val Lys Leu Val Glu Phe Thr 
65                  70                  75                  80  


Asp Tyr Val Arg Pro Asn Leu Ala Leu Ala Glu Gly Glu Leu Asp Ile 
                85                  90                  95      


Asn Val Phe Gln His Lys Pro Tyr Leu Asp Asp Phe Lys Lys Glu His 
            100                 105                 110         


Asn Leu Asp Ile Thr Glu Ala Phe Gln Val Pro Thr Ala Pro Leu Gly 
        115                 120                 125             


Leu Tyr Pro Gly Lys Leu Lys Ser Leu Glu Glu Val Lys Asp Gly Ser 
    130                 135                 140                 


Thr Val Ser Ala Pro Asn Asp Pro Ser Asn Phe Ala Arg Ala Leu Val 
145                 150                 155                 160 


Met Leu Asn Glu Leu Gly Trp Ile Lys Leu Lys Asp Gly Ile Asn Pro 
                165                 170                 175     


Leu Thr Ala Ser Lys Ala Asp Ile Ala Glu Asn Leu Lys Asn Ile Lys 
            180                 185                 190         


Ile Val Glu Leu Glu Ala Ala Gln Leu Pro Arg Ser Arg Ala Asp Val 
        195                 200                 205             


Asp Phe Ala Val Val Asn Gly Asn Tyr Ala Ile Ser Ser Gly Met Lys 
    210                 215                 220                 


Leu Thr Glu Ala Leu Phe Gln Glu Pro Ser Phe Ala Tyr Val Ala Trp 
225                 230                 235                 240 


Ser Ala Val Lys Thr Ala Asp Lys Asp Ser Gln Trp Leu Lys Asp Val 
                245                 250                 255     


Thr Glu Ala Tyr Asn Ser Asp Ala Phe Lys Ala Tyr Ala His Lys Arg 
            260                 265                 270         


Phe Glu Gly Tyr Lys Tyr Pro Ala Ala Trp Asn Glu Gly Ala Ala Lys 
        275                 280                 285             


<210>  4
<211>  1091
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae MetQSM DNA Sequence

<400>  4
atgcatccgg gcagcagcgc atcggctcgc acgaggtctg cgcttgaatt gtgttgtaga       60

aacacaacgt ttttgaaaaa ataagctatt gttttatatc aaaatataat catttttaaa      120

ataaaggttg cggcatttat cagatatttg ttctgaaatg aagacgtatc gggtgtttgc      180

ccgatgtttt taggttttta tcaaatttac aaaaggaagc ccatatgaaa accttcttca      240

aaaccctttc cgccgccgca ctcgcgctca tcctcgcagc ctgcggcggt caaaaagaca      300

gcgcgcccgc agcctctgcc gccgcccctt ctgccgataa cggcgcggcg aaaaaagaaa      360

tcgtcttcgg cacgaccgtc ggcgacttcg gcgatatggt caaagaacaa atccaagccg      420

agctggagaa aaaaggctac accgtcaaat tggtcgaatt taccgactat gtgcgcccga      480

atctggcatt ggcggagggc gagttggaca tcaacgtctt ccaacacaaa ccctatcttg      540

acgatttcaa aaaagaacac aacctggaca tcaccgaagc cttccaagtg ccgaccgcgc      600

ctttgggact gtatccgggc aaactgaaat cgctggaaga agtcaaagac ggcagcaccg      660

tatccgcgcc caacgacccg tccaacttcg cacgcgcctt ggtgatgctg aacgaactgg      720

gttggatcaa actcaaagac ggcatcaatc cgctgaccgc atccaaagcc gacatcgcgg      780

aaaacctgaa aaacatcaaa atcgtcgagc ttgaagccgc acaactgccg cgcagccgcg      840

ccgacgtgga ttttgccgtc gtcaacggca actacgccat aagcagcggc atgaagctga      900

ccgaagccct gttccaagag ccgagctttg cctatgtcgc ctggtctgcc gtcaaaaccg      960

ccgacaaaga cagccaatgg cttaaagacg taaccgaggc ctataactcc gacgcgttca     1020

aagcctacgc gcacaaacgc ttcgagggct acaaataccc tgccgcatgg aatgaaggcg     1080

cagccaaata a                                                          1091


<210>  5
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae GNA1220 protein.

<400>  5

Met Glu Phe Phe Ile Ile Leu Leu Ala Ala Val Ala Val Phe Gly Phe 
1               5                   10                  15      


Lys Ser Phe Val Val Ile Pro Gln Gln Glu Val His Val Val Glu Arg 
            20                  25                  30          


Leu Gly Arg Phe His Arg Ala Leu Thr Ala Gly Leu Asn Ile Leu Ile 
        35                  40                  45              


Pro Phe Ile Asp Arg Val Ala Tyr Arg His Ser Leu Lys Glu Ile Pro 
    50                  55                  60                  


Leu Asp Val Pro Ser Gln Val Cys Ile Thr Arg Asp Asn Thr Gln Leu 
65                  70                  75                  80  


Thr Val Asp Gly Ile Ile Tyr Phe Gln Val Thr Asp Pro Lys Leu Ala 
                85                  90                  95      


Ser Tyr Gly Ser Ser Asn Tyr Ile Met Ala Ile Thr Gln Leu Ala Gln 
            100                 105                 110         


Thr Thr Leu Arg Ser Val Ile Gly Arg Met Glu Leu Asp Lys Thr Phe 
        115                 120                 125             


Glu Glu Arg Asp Glu Ile Asn Ser Thr Val Val Ser Ala Leu Asp Glu 
    130                 135                 140                 


Ala Ala Gly Ala Trp Gly Val Lys Val Leu Arg Tyr Glu Ile Lys Asp 
145                 150                 155                 160 


Leu Val Pro Pro Gln Glu Ile Leu Arg Ala Met Gln Ala Gln Ile Thr 
                165                 170                 175     


Ala Glu Arg Glu Lys Arg Ala Arg Ile Ala Glu Ser Glu Gly Arg Lys 
            180                 185                 190         


Ile Glu Gln Ile Asn Leu Ala Ser Gly Gln Arg Glu Ala Glu Ile Gln 
        195                 200                 205             


Gln Ser Glu Gly Glu Ala Gln Ala Ala Val Asn Ala Ser Asn Ala Glu 
    210                 215                 220                 


Lys Ile Ala Arg Ile Asn Arg Ala Lys Gly Glu Ala Glu Ser Leu Arg 
225                 230                 235                 240 


Leu Val Ala Glu Ala Asn Ala Glu Ala Ile Arg Gln Ile Ala Ala Ala 
                245                 250                 255     


Leu Gln Thr Gln Gly Gly Ala Asp Ala Val Asn Leu Lys Ile Ala Glu 
            260                 265                 270         


Gln Tyr Val Ala Ala Phe Asn Asn Leu Ala Lys Glu Ser Asn Thr Leu 
        275                 280                 285             


Ile Met Pro Ala Asn Val Ala Asp Ile Gly Ser Leu Ile Ser Ala Gly 
    290                 295                 300                 


Met Lys Ile Ile Asp Ser Ser Lys Thr Ala Lys 
305                 310                 315 


<210>  6
<211>  948
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae GNA1220 DNA sequence.

<400>  6
atggaatttt tcattatctt gttggcagcc gtcgccgttt tcggcttcaa atcctttgtc       60

gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg ggcgtttcca tcgcgccctg      120

acggccggtt tgaatatttt gattcccttt atcgaccgcg tcgcctaccg ccattcgctg      180

aaagaaatcc ctttagacgt acccagccag gtctgcatca cgcgcgataa tacgcaattg      240

actgttgacg gcatcatcta tttccaagta accgatccca aactcgcctc atacggttcg      300

agcaactaca ttatggcaat tacccagctt gcccaaacga cgctgcgttc cgttatcggg      360

cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa tcaacagtac cgtcgtctcc      420

gccctcgatg aagccgccgg ggcttggggt gtgaaagtcc tccgttacga aatcaaggat      480

ttggttccgc cgcaagaaat ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa      540

aaacgcgccc gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt      600

ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc ggtcaatgcg      660

tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag gcgaagcgga atccctgcgc      720

cttgttgccg aagccaatgc cgaagccatc cgtcaaattg ccgccgccct tcaaacccaa      780

ggcggggcgg atgcggtcaa tctgaagatt gcggaacaat acgtagccgc gttcaacaat      840

cttgccaaag aaagcaatac gctgattatg cccgccaatg ttgccgacat cggcagcctg      900

atttctgccg gcatgaaaat tatcgacagc agcaaaaccg ccaaataa                   948


<210>  7
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae GNA1220aba protein sequence.

<400>  7

Met Val Asn Arg Thr Ala Phe Cys Leu Ser Leu Thr Ala Ala Leu Ile 
1               5                   10                  15      


Leu Thr Ala Cys Ser Ser Gly Gly Gly Gly Glu Gln Ile Asn Leu Ala 
            20                  25                  30          


Ser Gly Gln Arg Glu Ala Glu Ile Gln Gln Ser Glu Gly Glu Ala Gln 
        35                  40                  45              


Ala Ala Val Asn Ala Ser Asn Ala Glu Lys Ile Ala Arg Ile Asn Arg 
    50                  55                  60                  


Ala Lys Gly Glu Ala Glu Ser Leu Arg Leu Val Ala Glu Ala Asn Ala 
65                  70                  75                  80  


Glu Ala Ile Arg Gln Ile Ala Ala Ala Leu Gln Thr Gln Gly Gly Ala 
                85                  90                  95      


Asp Ala Val Asn Leu Lys Ile Ala Glu Gln Tyr Val Ala Ala Phe Asn 
            100                 105                 110         


Asn Leu Ala Lys Glu Ser Asn Thr Leu Ile Met Pro Ala Asn Val Ala 
        115                 120                 125             


Asp Ile Gly Ser Leu Ile Ser Ala Gly Met Lys Ile Ile Asp Ser Ser 
    130                 135                 140                 


Lys Thr Ala Lys 
145             


<210>  8
<211>  447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae GNA1220_helix-aba DNA sequence.

<400>  8
atggtgaacc gaactgcctt ctgcctttct ctgaccgccg ccctgattct gaccgcctgc       60

agcagcggag ggggtggtga acaaatcaac cttgccagtg gtcagcgtga agccgaaatc      120

caacaatccg aaggcgaggc tcaggctgcg gtcaatgcgt ccaatgccga gaaaatcgcc      180

cgcatcaacc gcgccaaagg cgaagcggaa tccctgcgcc ttgttgccga agccaatgcc      240

gaagccatcc gtcaaattgc cgccgccctt caaacccaag gcggggcgga tgcggtcaat      300

ctgaagattg cggaacaata cgtagccgcg ttcaacaatc ttgccaaaga aagcaatacg      360

ctgattatgc ccgccaatgt tgccgacatc ggcagcctga tttctgccgg catgaaaatt      420

atcgacagca gcaaaaccgc caaataa                                          447


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MetQ_neisseria forward primer

<400>  9
atacaattgc ctcagcgcat gcatc                                             25


<210>  10
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MetQ_SbfI reverse primer

<400>  10
tatcctgcag gttatacgac tgccttattt g                                      31


<210>  11
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MetQ_neisseria reverse primer

<400>  11
tattctagat tatacgactg ccttatttgg c                                      31


<210>  12
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  MetQ_SpeI reverse primer

<400>  12
tatactagtt tatacgactg ccttatttgg ctg                                    33


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GNA1220_StuI reverse primer

<400>  13
tataggcctt atacgactgc cttatttggc                                        30


<210>  14
<211>  2260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blue script Plasmid (FHbp KO+MetQ)

<400>  14
atggcactcg tatccatgcg ccaactgctt gatcatgctg ccgaaaacag ctacggcctg       60

ccggcgttca acgtcaacaa cctcgaacag atgcgcgcca tcatggaggc tgcagaccaa      120

gtcgacgccc ccgtcatcgt acaggcgagt gccggtgcgc gcaaatatgc gggtgcgccg      180

tttttacgcc acctgatttt ggcggctgtc gaagaatttc cacacatccc gtcgtcatgc      240

accaagacca cggcgcatca cccgacgtgt gccaacgctc catccaactg ggcttctcct      300

ctgtaatgat ggacggctcg ctgatggaag acggcaaaac cccttcttct tacgaataca      360

acgtcaacgc cacacgtacc gtggttaact tctcccacgc ttgcggcgta tccgttgaag      420

gcgaaatcgg cgtattgggc aacctcgaaa ccggcgaagc aggcgaagaa gacggtgtag      480

gcgcagtggg caaactttcc cacgaccaaa tgctgaccag cgtcgaagat gccgtatgtt      540

tcgttaaaga taccggcgtt gacgcattgg ctattgccgt cggcaccagc cacggcgcat      600

acaaattcac ccgtccgccc acaggcgatg tattacgtat cgaccgcatc aaagaaatcc      660

accaagccct gcccaataca cacatcgtga tgcacggctc cagctccgtt ccgcaagaat      720

ggctgaaagt catcaacgaa tacggcggca atatcggcga aacctacggc gtgccggttg      780

aagaaatcgt cgaaggcatc aaacacggcg tgcgcaaagt caacatcgat accgacttgc      840

gccttgcttc taccggcgcg gtacgccgct accttgccga aaatccgtcc gactttgacc      900

cgcgcaaata cctgagcaaa accattgagg ccatgaagca aatctgcctc gaccgttatc      960

ttgcgtttgg ctgcgaaggt caggcaggca aaatcaaacc tgtttcgttg gaaaaaatgg     1020

caagccgtta tgccaagggc gaattgaacc aaatcgtcaa ataacaggtt gcctgtaaac     1080

aaaatgccgt cccatgggca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt     1140

gtgtataata ggaattgaag ttaaattaga tgctaaaaat ttgtaattaa gaaggaggga     1200

ttcgtcatgt tggtattcca aatgcgtaat gtagataaaa catctactgt tttgaaacag     1260

actaaaaaca gtgattacgc agataaataa atacgttaga ttaattccta ccagtgacta     1320

atcttatgac tttttaaaca gataactaaa attacaaaca aatcgtttaa cttctgtatt     1380

tatttacaga tgtaatcact tcaggagtaa ttacatgaac aaaaatataa aatattctca     1440

aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga     1500

aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa     1560

aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga     1620

aaaattaaaa ctgaacattc gtgtcacttt aattcaccaa gatattctac agtttcaatt     1680

ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat     1740

tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg     1800

attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt     1860

ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa     1920

cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct     1980

atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa     2040

tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtacca ttacttatga     2100

gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga     2160

gtcgcttttt taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag     2220

atatactact gacagcttcc aagaagctaa agaggtccct                           2260


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  metQ pBS downstream forward primer

<400>  15
ccctgttcca agagccgagc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RBD pBS downstream reverse primer

<400>  16
agcttcttcc agcgcgaacg                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FHbp upstream forward primer

<400>  17
ggcgaaatcg gcgtattggg                                                   20


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FHbp upstream reverse primer

<400>  18
ctacattacg catttggaat acc                                               23


<210>  19
<211>  5330
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM Plasmid (Capsule KO+MetQ)

<400>  19
ctattttcca gtacctaaac gttccaaacg atagctgccg ttcaatacat tttgccacca       60

ggttttgttg tccagatacc attgcacggt tttgcggagg ccggactcga aggtttccaa      120

aggcagccag cccaaatccc gcctgatttt ggctgcgtcg acggcgtagc gtacgtcatg      180

gccggggcgg tcttgtacga aagtaatcaa atcttcataa cgcgccacac cggccggttt      240

ttcgggagcg agttcttcca gcagggcgca gatggttttg acgacttcaa tattggcttt      300

ttcattgtgg ccgccgatat tgtaggtttc gccgacaaca ccttcggtaa caacctgata      360

cagtgcgcgc gcgtggtctt cgacaaacag ccagtcgcgg atttgcatac cgtcgccgta      420

cacaggcagc ggtttgccgt caagcgcgtt cagaatcatc aaaggaatga gtttttccgg      480

aaaatggtaa ggaccgtagt tgttggagca gttggttaca atggtcggca agccgtaagt      540

acgcaaccac gcgcggacga ggtggtcgct ggacacttta gaggcagagt aggggctgga      600

cggcgcgtag ggcgcggttt cggtaaacaa atcgtccgtg ccgcctaaat cgccatagac      660

ttcatcggtg gaaatatggt ggaaacggaa ggcttcgtgc tgttcagacg gcatttgttg      720

ccagtaggcg cgggctgctt caagcagatt gaatgtgccg acgatattgg tttggataaa      780

ctcgcctgcc gaaccgatag agcggtcgac atggctttcc gccgccaagt gcatcacggc      840

atcgggccgg tgttgtgcga atacgcggtc gagttcggcg cggtcgcaaa tgtccacttg      900

ctcgaaagca tagcggggat tatcggcaat gtcggtcagc gattcgagat tgcccgcgta      960

ggtcagtttg tcgaggttga cgacggaatc ttgggtgttt cggataatat gacggacaac     1020

ggcagaaccg ataaagcccg caccgccggt aacgaggatg gtttttttgt ttgcggtttg     1080

catttaatcc ccatatccgt tggggttgcg gctgacccaa cgccacgaat cttccatcat     1140

ttgctgcaag ccgcgtttgg tttcccagcc ggtttgttgt ttggtatggg acgggtcggc     1200

aaaggaacac gccaaatcac cggcgcggcg cggtttgatt tcaaatggaa tggttaaacc     1260

cgaagccgct tcaaatgcgc ggatgatttc caataccgaa gaagcgcggc cggagcctaa     1320

gttcagcaaa tgcgtgcctg ctacattact ttttgcctgc atagccgcga catggccttc     1380

tgccaaatcc atcacatgaa tatagtcacg catccccgtg ccgtcggggg tagggtagtc     1440

atcgccaaat accgccaatt gcctcagcgc atgcatccgg gcagcagcgc atcggctcgc     1500

acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt ttttgaaaaa ataagctatt     1560

gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat cagatatttg     1620

ttctgaaatg aagacgtatc gggtgtttgc ccgatgtttt taggttttta tcaaatttac     1680

aaaaggaagc ccatatgaaa accttcttca aaaccctttc cgccgccgca ctcgcgctca     1740

tcctcgcagc ctgcggcggt caaaaagaca gcgcgcccgc agcctctgcc gccgcccctt     1800

ctgccgataa cggcgcggcg aaaaaagaaa tcgtcttcgg cacgaccgtc ggcgacttcg     1860

gcgatatggt caaagaacaa atccaagccg agctggagaa aaaaggctac accgtcaaat     1920

tggtcgaatt taccgactat gtgcgcccga atctggcatt ggcggagggc gagttggaca     1980

tcaacgtctt ccaacacaaa ccctatcttg acgatttcaa aaaagaacac aacctggaca     2040

tcaccgaagc cttccaagtg ccgaccgcgc ctttgggact gtatccgggc aaactgaaat     2100

cgctggaaga agtcaaagac ggcagcaccg tatccgcgcc caacgacccg tccaacttcg     2160

cacgcgcctt ggtgatgctg aacgaactgg gttggatcaa actcaaagac ggcatcaatc     2220

cgctgaccgc atccaaagcc gacatcgcgg aaaacctgaa aaacatcaaa atcgtcgagc     2280

ttgaagccgc acaactgccg cgcagccgcg ccgacgtgga ttttgccgtc gtcaacggca     2340

actacgccat aagcagcggc atgaagctga ccgaagccct gttccaagag ccgagctttg     2400

cctatgtcaa ctggtctgcc gtcaaaaccg ccgacaaaga cagccaatgg cttaaagacg     2460

taaccgaggc ctataactcc gacgcgttca aagcctacgc gcacaaacgc ttcgagggct     2520

acaaataccc tgccgcatgg aatgaaggcg cagccaaata aggcagtcgt ataatctaga     2580

gacgatgccg tctgaagaaa ccttgcgctc gttcgccagc caggacagaa atgcctcgac     2640

ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga aggcacgaac     2700

ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg     2760

caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat     2820

ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc     2880

gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg     2940

cagtcgccct aaaacaaagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac     3000

tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt     3060

acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct     3120

ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt     3180

ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt     3240

tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga     3300

atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct     3360

ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga     3420

ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac     3480

gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc     3540

ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg     3600

ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta     3660

tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca     3720

ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctatgcc gtctgaatac     3780

tcgagcggaa cccgggtagt ggcctgtaaa agacagcata tagagatgag caggctgtat     3840

aatattaagg atttttttgt aacttctata aatataaagt aattttttag gagttatatt     3900

attagggctt ctaggaagct caaatagata aatagattca aatagattct tgttagctga     3960

ttgatgaact aacttaggca tttttaagtt tttagaagta tataaaatta ctagtaaatt     4020

attggttaat ttttgtattt taattaggct ttggacttgg ttaagctgac ctaaattaga     4080

tatgacaaat aaattgttac gtgggggggt aagataaaat ggagatgttg tcaaccacat     4140

tgaatcttga aaaaactttt taggctgaaa aagagctttt tttattttct ttagcattat     4200

tgtatctctt aaaaattaat gagaattagc tatatgtaat agccaatcct ctgttaataa     4260

agtaactaag ttaataagca ttattcaata tcagtttttt tgatttgagc acctttgcga     4320

atattgcaag cagcgacctt accaaataat gtttcatatt cgttgacgct gaagtctcca     4380

ttgcctgggc gtttaaccca taggttatct ccggacaaca gttctccttt tttaatgtct     4440

ttatctgcta cgacagatgc aaaggcgaaa tctttagttg gcttttctcc cgcgataatc     4500

gtgtcttttt tgccgccgcg tgccaatttt aaagcatgag cgccttgctt gagctcttta     4560

aaagtatccg gattcataga gcatacaata tccggacctg ggcgatccat gcggtcagta     4620

aagtgacgct ctaaaatcga accgcctaaa gctactgctc ctaagcaagc atagttatct     4680

aaggtatggt cagacaggcc aatgattgcg tctggaaagg cttcagataa atcgttcata     4740

ccacccaatc gaacatcttc gtaaggggtt gggtagatgt tggtacagtg aagcaaagca     4800

taaggtaccc ctgcttctcg aataatttct accgactttt tgatgctttc aatagaattc     4860

atgccggtag agagaataat aggcttacca aaagaggcca ccagtttaat taatgggtag     4920

ttattacatt cgccagagcc gattttatat gctggaatat ccatacgttg taatcgtaaa     4980

gcagctgcac gagagaaagg agtactgata aaaatcatac ccttactctc tacgtattct     5040

tttaatttaa tctcatcttc ttcattcagg gcgcaacgtt ccataatttc ataaatagag     5100

acatctgcat tgcctggaat gacttgtttg gcctcatcag acatttcgtc ttcaacgatg     5160

tgtgtttgat gtttaacaac ttcagcgcct gcattatagg cagcatcaac catttcaaaa     5220

gctgttttta aagagccttc atgattgatg ccgatttcac agataatcaa tggttcgtgg     5280

ttgtaaccta ctgaacgatt accaatttta aattcgttgt tgttttgcat                5330


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Capsule KO GalE forward primer

<400>  20
atacgcggtc gagttcggcg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Capsule KO upstream metQ Reverse primer

<400>  21
gcgcgctgtc tttttgaccg                                                   20


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Capsule KO Spc downstream forward primer

<400>  22
gatgagcgaa atgtagtgct tacg                                              24


<210>  23
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Capsule KO SiaD reverse primer

<400>  23
gcatctgtcg tagcagataa agac                                              24


<210>  24
<211>  3392
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18 Plasmid (lpxL1 KO+MetQ)

<400>  24
atgtcggcaa ccgtccgtat cttgggcatt gacccgggca gccgcgtaac gggtttcggc       60

atcatcgatg tcagggggcg cgatcatttt tacgttgcct caggctgcat caaaacgcct      120

gccgatgcgc ctctggcaga caggattgcc gtcatcgtcc ggcacatcgg cgaagtcgtt      180

accgtttaca agcctcaaca ggcggcggtg gaacaggtgt tcgtcaacgt caatcctgca      240

tcgacgctga tgctcggtca ggcgcggggc gcggcattgg cggcattggt cagccataag      300

ctgcccgttt cggaatacac ggccttgcag gtcaaacagg cggtggtcgg caagggtaag      360

gcagcgaaag aacaggtgca gcatatggtg gtgcagatgc tggggctttc gggaacaccg      420

caggcggatg cggcggacgg tcttgccgtc gcgctgaccc acgccttacg caaccacggg      480

cttgccgcca aactcaatcc ttcagggatg caggtcaagc gcggaaggtt tcaatagttt      540

cagacggcat ttgtattttg ccgtctgaaa agaaaatgtg tatcgagatg aaatttatat      600

ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga ttgccgacct      660

gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg aaatcaattt      720

ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga aacagcattt      780

caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc ctgccggacg      840

tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc tggcggcggg      900

ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttgcagtga ctaactagga      960

ggaataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg     1020

ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa     1080

tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga     1140

acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca     1200

ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg     1260

ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg     1320

catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag     1380

ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa     1440

ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga     1500

aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt     1560

gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg     1620

gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt     1680

cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat     1740

tttactggat gaattgtttt agtacccctc agcgcatgca tccgggctct gcgcttgaat     1800

tgtgttgtag aaacacaacg tttttgaaaa aataagctat tgttttatat caaaatataa     1860

tcatttttaa aataaaggtt gcggcattta tcagatattt gttctgaaat gaagacgtat     1920

cgggtgtttg cccgatgttt ttaggttttt atcaaattta caaaaggaag cccatatgaa     1980

aaccttcttc aaaacccttt ccgccgccgc actcgcgctc atcctcgcag cctgcggcgg     2040

tcaaaaagac agcgcgcccg cagcctctgc cgccgcccct tctgccgata acggcgcggc     2100

gaaaaaagaa atcgtcttcg gcacgaccgt cggcgacttc ggcgatatgg tcaaagaaca     2160

aatccaagcc gagctggaga aaaaaggcta caccgtcaaa ttggtcgaat ttaccgacta     2220

tgtgcgcccg aatctggcat tggcggaggg cgagttggac atcaacgtct tccaacacaa     2280

accctatctt gacgatttca aaaaagaaca caacctggac atcaccgaag ccttccaagt     2340

gccgaccgcg cctttgggac tgtatccggg caaactgaaa tcgctggaag aagtcaaaga     2400

cggcagcacc gtatccgcgc ccaacgaccc gtccaacttc gcacgcgcct tggtgatgct     2460

gaacgaactg ggttggatca aactcaaaga cggcatcaat ccgctgaccg catccaaagc     2520

cgacatcgcg gaaaacctga aaaacatcaa aatcgtcgag cttgaagccg cacaactgcc     2580

gcgcagccgc gccgacgtgg attttgccgt cgtcaacggc aactacgcca taagcagcgg     2640

catgaagctg accgaagccc tgttccaaga gccgagcttt gcctatgtca actggtctgc     2700

cgtcaaaacc gccgacaaag acagccaatg gcttaaagac gtaaccgagg cctataactc     2760

cgacgcgttc aaagcctacg cgcacaaacg cttcgagggc tacaaatacc ctgccgcatg     2820

gaatgaaggc gcagccaaat aaggcagtcg tataacctgc agggaataat gagtcgacag     2880

gatttcggac gcaacgattc ggtttttgtg gattttttcg gtattcagac ggcaacgatt     2940

accggattga gccgcattgc cgcgcttgca aatgcaaaag tgatacccgc cattcccgtc     3000

cgcgaggcag acaatacggt tacattgcat ttctaccctg cttggaaatc ctttccgggt     3060

gaagacgcga aagccgacgc gcagcgcatg aaccgtttta tcgaagacag ggtgcgcgaa     3120

catccggaac aatatttttg gctgcacaag cgttttaaaa cccgtccgga aggcagcccc     3180

gatttttact gactacgtaa aattacaaaa catatcaggc gtttcgagtc gaaactcctg     3240

attgtttttt taaatgcaaa atggcagatt atatgaacag atttcatttg ataatcgctg     3300

ctatttaagt atctcaaaaa acaatatttt taagacttgg tcgggaaatt cgaagcaggt     3360

tttggaagaa ataccgcaaa cctatgaata ta                                   3392


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lpxl1 upstream Forward primer

<400>  25
gtcggcaagg gtaaggcagc                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lpxl1 upstream reverse primer

<400>  26
ccctttatac cggctgtccg                                                   20


<210>  27
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Lpxl1 downstream reverse primer

<400>  27
atattcatag gtttgcggta tttc                                              24


<210>  28
<211>  2261
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Blue script Plasmid (FHbp KO+GNA1220)

<400>  28
atggcactcg tatccatgcg ccaactgctt gatcatgctg ccgaaaacag ctacggcctg       60

ccggcgttca acgtcaacaa cctcgaacag atgcgcgcca tcatggaggc tgcagaccaa      120

gtcgacgccc ccgtcatcgt acaggcgagt gccggtgcgc gcaaatatgc gggtgcgccg      180

tttttacgcc acctgatttt ggcggctgtc gaagaatttc cacacatccc cgtcgtcatg      240

caccaagacc acggcgcatc acccgacgtg tgccaacgct ccatccaact gggcttctcc      300

tctgtaatga tggacggctc gctgatggaa gacggcaaaa ccccttcttc ttacgaatac      360

aacgtcaacg ccacacgtac cgtggttaac ttctcccacg cttgcggcgt atccgttgaa      420

ggcgaaatcg gcgtattggg caacctcgaa accggcgaag caggcgaaga agacggtgta      480

ggcgcagtgg gcaaactttc ccacgaccaa atgctgacca gcgtcgaaga tgccgtatgt      540

ttcgttaaag ataccggcgt tgacgcattg gctattgccg tcggcaccag ccacggcgca      600

tacaaattca cccgtccgcc cacaggcgat gtattacgta tcgaccgcat caaagaaatc      660

caccaagccc tgcccaatac acacatcgtg atgcacggct ccagctccgt tccgcaagaa      720

tggctgaaag tcatcaacga atacggcggc aatatcggcg aaacctacgg cgtgccggtt      780

gaagaaatcg tcgaaggcat caaacacggc gtgcgcaaag tcaacatcga taccgacttg      840

cgccttgctt ctaccggcgc ggtacgccgc taccttgccg aaaatccgtc cgactttgac      900

ccgcgcaaat acctgagcaa aaccattgag gccatgaagc aaatctgcct cgaccgttat      960

cttgcgtttg gctgcgaagg tcaggcaggc aaaatcaaac ctgtttcgtt ggaaaaaatg     1020

gcaagccgtt atgccaaggg cgaattgaac caaatcgtca aataacaggt tgcctgtaaa     1080

caaaatgccg tcccatgggc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt     1140

tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg     1200

attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca     1260

gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact     1320

aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttctgtat     1380

ttatttacag atgtaatcac ttcaggagta attacatgaa caaaaatata aaatattctc     1440

aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg aatttaaaag     1500

aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg aaactggcta     1560

aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac ttatcgtcag     1620

aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta cagtttcaat     1680

tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta agcacacaaa     1740

ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt gttgaagaag     1800

gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg cacactcaag     1860

tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa ccaaaagtaa     1920

acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa tattggaagc     1980

tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg tttactaaaa     2040

atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc attacttatg     2100

agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa taattctatg     2160

agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga taaattatta     2220

gatatactac tgacagcttc caagaagcta aagaggtccc t                         2261


<210>  29
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GNA1220 pBS downstream forward primer

<400>  29
cttgccaaag aaagcaatac gctg                                              24


<210>  30
<211>  5411
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM Plasmid (Capsule KO+GNA1220)

<400>  30
ctattttcca gtacctaaac gttccaaacg atagctgccg ttcaatacat tttgccacca       60

ggttttgttg tccagatacc attgcacggt tttgcggagg ccggactcga aggtttccaa      120

aggcagccag cccaaatccc gcctgatttt ggctgcgtcg acggcgtagc gtacgtcatg      180

gccggggcgg tcttgtacga aagtaatcaa atcttcataa cgcgccacac cggccggttt      240

ttcgggagcg agttcttcca gcagggcgca gatggttttg acgacttcaa tattggcttt      300

ttcattgtgg ccgccgatat tgtaggtttc gccgacaaca ccttcggtaa caacctgata      360

cagtgcgcgc gcgtggtctt cgacaaacag ccagtcgcgg atttgcatac cgtcgccgta      420

cacaggcagc ggtttgccgt caagcgcgtt cagaatcatc aaaggaatga gtttttccgg      480

aaaatggtaa ggaccgtagt tgttggagca gttggttaca atggtcggca agccgtaagt      540

acgcaaccac gcgcggacga ggtggtcgct ggacacttta gaggcagagt aggggctgga      600

cggcgcgtag ggcgcggttt cggtaaacaa atcgtccgtg ccgcctaaat cgccatagac      660

ttcatcggtg gaaatatggt ggaaacggaa ggcttcgtgc tgttcagacg gcatttgttg      720

ccagtaggcg cgggctgctt caagcagatt gaatgtgccg acgatattgg tttggataaa      780

ctcgcctgcc gaaccgatag agcggtcgac atggctttcc gccgccaagt gcatcacggc      840

atcgggccgg tgttgtgcga atacgcggtc gagttcggcg cggtcgcaaa tgtccacttg      900

ctcgaaagca tagcggggat tatcggcaat gtcggtcagc gattcgagat tgcccgcgta      960

ggtcagtttg tcgaggttga cgacggaatc ttgggtgttt cggataatat gacggacaac     1020

ggcagaaccg ataaagcccg caccgccggt aacgaggatg gtttttttgt ttgcggtttg     1080

catttaatcc ccatatccgt tggggttgcg gctgacccaa cgccacgaat cttccatcat     1140

ttgctgcaag ccgcgtttgg tttcccagcc ggtttgttgt ttggtatggg acgggtcggc     1200

aaaggaacac gccaaatcac cggcgcggcg cggtttgatt tcaaatggaa tggttaaacc     1260

cgaagccgct tcaaatgcgc ggatgatttc caataccgaa gaagcgcggc cggagcctaa     1320

gttcagcaaa tgcgtgcctg ctacattact ttttgcctgc atagccgcga catggccttc     1380

tgccaaatcc atcacatgaa tatagtcacg catccccgtg ccgtcggggg tagggtagtc     1440

atcgccaaat accgccaatt gcctcagcgc atgcatccgg gcagcagcgc atcggctcgc     1500

acgaggtctg cgcttgaatt gtgttgtaga aacacaacgt ttttgaaaaa ataagctatt     1560

gttttatatc aaaatataat catttttaaa ataaaggttg cggcatttat cagatatttg     1620

ttctgaaatg aagacgtatc gggtgtttgc ccgatgtttt taggttttta tcaaatttac     1680

aaaaggaagc ccatatggaa tttttcatta tcttgttggc agccgtcgcc gttttcggct     1740

tcaaatcctt tgtcgtcatc ccccagcagg aagtccacgt tgtcgaaagg ctcgggcgtt     1800

tccatcgcgc cctgacggcc ggtttgaata ttttgattcc ctttatcgac cgcgtcgcct     1860

accgccattc gctgaaagaa atccctttag acgtacccag ccaggtctgc atcacgcgcg     1920

ataatacgca attgactgtt gacggcatca tctatttcca agtaaccgat cccaaactcg     1980

cctcatacgg ttcgagcaac tacattatgg caattaccca gcttgcccaa acgacgctgc     2040

gttccgttat cgggcgtatg gagttggaca aaacgtttga agaacgcgac gaaatcaaca     2100

gtaccgtcgt ctccgccctc gatgaagccg ccggggcttg gggtgtgaaa gtcctccgtt     2160

acgaaatcaa ggatttggtt ccgccgcaag aaatccttcg cgcaatgcag gcacaaatta     2220

ccgccgaacg cgaaaaacgc gcccgtattg ccgaatccga aggccgtaaa atcgaacaaa     2280

tcaaccttgc cagtggtcag cgtgaagccg aaatccaaca atccgaaggc gaggctcagg     2340

ctgcggtcaa tgcgtccaat gccgagaaaa tcgcccgcat caaccgcgcc aaaggcgaag     2400

cggaatccct gcgccttgtt gccgaagcca atgccgaagc catccgtcaa attgccgccg     2460

cccttcaaac ccaaggcggg gcggatgcgg tcaatctgaa gattgcggaa caatacgtag     2520

ccgcgttcaa caatcttgcc aaagaaagca atacgctgat tatgcccgcc aatgttgccg     2580

acatcggcag cctgatttct gccggcatga aaattatcga cagcagcaaa accgccaaat     2640

aaggcagtcg tataatctag agacgatgcc gtctgaagaa accttgcgct cgttcgccag     2700

ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg     2760

gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta agctgtaatg     2820

caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca gcggtggtaa     2880

cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac agtctatgcc     2940

tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag     3000

caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca tgagggaagc     3060

ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct     3120

cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc     3180

acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg     3240

agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg     3300

cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa     3360

gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc     3420

agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc     3480

cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga     3540

ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg     3600

aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc     3660

gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat     3720

acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga     3780

tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag tcggcaaata     3840

atgtctatgc cgtctgaata ctcgagcgga acccgggtag tggcctgtaa aagacagcat     3900

atagagatga gcaggctgta taatattaag gatttttttg taacttctat aaatataaag     3960

taatttttta ggagttatat tattagggct tctaggaagc tcaaatagat aaatagattc     4020

aaatagattc ttgttagctg attgatgaac taacttaggc atttttaagt ttttagaagt     4080

atataaaatt actagtaaat tattggttaa tttttgtatt ttaattaggc tttggacttg     4140

gttaagctga cctaaattag atatgacaaa taaattgtta cgtggggggg taagataaaa     4200

tggagatgtt gtcaaccaca ttgaatcttg aaaaaacttt ttaggctgaa aaagagcttt     4260

ttttattttc tttagcatta ttgtatctct taaaaattaa tgagaattag ctatatgtaa     4320

tagccaatcc tctgttaata aagtaactaa gttaataagc attattcaat atcagttttt     4380

ttgatttgag cacctttgcg aatattgcaa gcagcgacct taccaaataa tgtttcatat     4440

tcgttgacgc tgaagtctcc attgcctggg cgtttaaccc ataggttatc tccggacaac     4500

agttctcctt ttttaatgtc tttatctgct acgacagatg caaaggcgaa atctttagtt     4560

ggcttttctc ccgcgataat cgtgtctttt ttgccgccgc gtgccaattt taaagcatga     4620

gcgccttgct tgagctcttt aaaagtatcc ggattcatag agcatacaat atccggacct     4680

gggcgatcca tgcggtcagt aaagtgacgc tctaaaatcg aaccgcctaa agctactgct     4740

cctaagcaag catagttatc taaggtatgg tcagacaggc caatgattgc gtctggaaag     4800

gcttcagata aatcgttcat accacccaat cgaacatctt cgtaaggggt tgggtagatg     4860

ttggtacagt gaagcaaagc ataaggtacc cctgcttctc gaataatttc taccgacttt     4920

ttgatgcttt caatagaatt catgccggta gagagaataa taggcttacc aaaagaggcc     4980

accagtttaa ttaatgggta gttattacat tcgccagagc cgattttata tgctggaata     5040

tccatacgtt gtaatcgtaa agcagctgca cgagagaaag gagtactgat aaaaatcata     5100

cccttactct ctacgtattc ttttaattta atctcatctt cttcattcag ggcgcaacgt     5160

tccataattt cataaataga gacatctgca ttgcctggaa tgacttgttt ggcctcatca     5220

gacatttcgt cttcaacgat gtgtgtttga tgtttaacaa cttcagcgcc tgcattatag     5280

gcagcatcaa ccatttcaaa agctgttttt aaagagcctt catgattgat gccgatttca     5340

cagataatca atggttcgtg gttgtaacct actgaacgat taccaatttt aaattcgttg     5400

ttgttttgca t                                                          5411


<210>  31
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Capsule KO upstream GNA1220 R

<400>  31
tgaagccgaa aacggcgacg                                                   20


<210>  32
<211>  3498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18 Plasmid (lpxL1 KO+GNA1220)

<400>  32
atgtcggcaa ccgtccgtat cttgggcatt gacccgggca gccgcgtaac gggtttcggc       60

atcatcgatg tcagggggcg cgatcatttt tacgttgcct caggctgcat caaaacgcct      120

gccgatgcgc ctctggcaga caggattgcc gtcatcgtcc ggcacatcgg cgaagtcgtt      180

accgtttaca agcctcaaca ggcggcggtg gaacaggtgt tcgtcaacgt caatcctgca      240

tcgacgctga tgctcggtca ggcgcggggc gcggcattgg cggcattggt cagccataag      300

ctgcccgttt cggaatacac ggccttgcag gtcaaacagg cggtggtcgg caagggtaag      360

gcagcgaaag aacaggtgca gcatatggtg gtgcagatgc tggggctttc gggaacaccg      420

caggcggatg cggcggacgg tcttgccgtc gcgctgaccc acgccttacg caaccacggg      480

cttgccgcca aactcaatcc ttcagggatg caggtcaagc gcggaaggtt tcaatagttt      540

cagacggcat ttgtattttg ccgtctgaaa agaaaatgtg tatcgagatg aaatttatat      600

tttttgtact gtatgttttg cagtttctgc cgtttgcgct gctgcacaag attgccgacc      660

tgacgggttt gcttgcctac cttctggtca aaccgcgccg ccgtatcggc gaaatcaatt      720

tggcaaaatg tttttccgaa tggagtgagg aaaagcgtaa aaccgtgttg aaacagcatt      780

tcaaacacat ggcgaaactg atgttggaat acggtttata ttggtacgcg cctgccggac      840

gtttgaaatc gctggtgcgc taccgcaata agcattattt ggacgacgcg ctggcggcgg      900

gggaaaaagt catcatcctg tatccgcact tcaccgctgc agttgcagtg actaactagg      960

aggaataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc     1020

gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa     1080

atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg     1140

aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc     1200

actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt     1260

gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt     1320

gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca     1380

gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa     1440

actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg     1500

aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg     1560

tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt     1620

ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg     1680

tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata     1740

ttttactgga tgaattgttt tagtacccct cagcgcatgc atccgggcag cagcgcatcg     1800

gctcgcacga ggtctgcgct tgaattgtgt tgtagaaaca caacgttttt gaaaaaataa     1860

gctattgttt tatatcaaaa tataatcatt tttaaaataa aggttgcggc atttatcaga     1920

tatttgttct gaaatgaaga cgtatcgggt gtttgcccga tgtttttagg tttttatcaa     1980

atttacaaaa ggaagcccat atggaatttt tcattatctt gttggcagcc gtcgccgttt     2040

tcggcttcaa atcctttgtc gtcatccccc agcaggaagt ccacgttgtc gaaaggctcg     2100

ggcgtttcca tcgcgccctg acggccggtt tgaatatttt gattcccttt atcgaccgcg     2160

tcgcctaccg ccattcgctg aaagaaatcc ctttagacgt acccagccag gtctgcatca     2220

cgcgcgataa tacgcaattg actgttgacg gcatcatcta tttccaagta accgatccca     2280

aactcgcctc atacggttcg agcaactaca ttatggcaat tacccagctt gcccaaacga     2340

cgctgcgttc cgttatcggg cgtatggagt tggacaaaac gtttgaagaa cgcgacgaaa     2400

tcaacagtac cgtcgtctcc gccctcgatg aagccgccgg ggcttggggt gtgaaagtcc     2460

tccgttacga aatcaaggat ttggttccgc cgcaagaaat ccttcgcgca atgcaggcac     2520

aaattaccgc cgaacgcgaa aaacgcgccc gtattgccga atccgaaggc cgtaaaatcg     2580

aacaaatcaa ccttgccagt ggtcagcgtg aagccgaaat ccaacaatcc gaaggcgagg     2640

ctcaggctgc ggtcaatgcg tccaatgccg agaaaatcgc ccgcatcaac cgcgccaaag     2700

gcgaagcgga atccctgcgc cttgttgccg aagccaatgc cgaagccatc cgtcaaattg     2760

ccgccgccct tcaaacccaa ggcggggcgg atgcggtcaa tctgaagatt gcggaacaat     2820

acgtagccgc gttcaacaat cttgccaaag aaagcaatac gctgattatg cccgccaatg     2880

ttgccgacat cggcagcctg atttctgccg gcatgaaaat tatcgacagc agcaaaaccg     2940

ccaaataagg cagtcgtata acctgcaggg aataatgagt cgacaggatt tcggacgcaa     3000

cgattcggtt tttgtggatt ttttcggtat tcagacggca acgattaccg gattgagccg     3060

cattgccgcg cttgcaaatg caaaagtgat acccgccatt cccgtccgcg aggcagacaa     3120

tacggttaca ttgcatttct accctgcttg gaaatccttt ccgggtgaag acgcgaaagc     3180

cgacgcgcag cgcatgaacc gttttatcga agacagggtg cgcgaacatc cggaacaata     3240

tttttggctg cacaagcgtt ttaaaacccg tccggaaggc agccccgatt tttactgact     3300

acgtaaaatt acaaaacata tcaggcgttt cgagtcgaaa ctcctgattg tttttttaaa     3360

tgcaaaatgg cagattatat gaacagattt catttgataa tcgctgctat ttaagtatct     3420

caaaaaacaa tatttttaag acttggtcgg gaaattcgaa gcaggttttg gaagaaatac     3480

cgcaaaccta tgaatata                                                   3498


<210>  33
<211>  6740
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-MetQ plasmid

<400>  33
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcat aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccaat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cttataacag gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt tttttcagga tttaaaatta caaaaccctt acagagcaag     1440

taaacttgtt tgcttgttct gcaagggttc agcaaccgta gccgtcaggc gtagggcggt     1500

agcctataaa agccatttaa ttttatcttt aaacttcctt ttaaatgctt tgagtgggtg     1560

tcttttatcg tactcatcaa tccttttttg cattctttcg tttgctttgt gatcggcaaa     1620

ttttgaataa gatttttcca tctcatctaa cattctatca atccgttttt tatgttgcca     1680

tttcaggtaa acataaacac ttatagcaat taaagacaat atcaatacat tgtaaaaaat     1740

gattgttaca atttcgctca cagttatttt ttaccttttt caatttcttc attgataaat     1800

gcactcaatt catcaaattt cttgtcatca ttgataaatt tacgcaactt agggaagttt     1860

ctatctacat ctaaaagagg gttatttatt atttcattta gccaaaaagc ccctaataaa     1920

accttgtaat gcgtagcttt cttacgcttt tctgcttgtt cttttgactt aatcgcacga     1980

attttcgctt tgatttcgtc ctgcttgcgt tgtaaatctg cttgttgctg ttccaatctt     2040

gtaagttttt cgcttgccat actagcccct ttatatagtt agaaattatc gttattttat     2100

tcagtaggtg ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac     2160

ttatcagttt ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac     2220

tcaacactat ggcaatctat catttaaacg ttcgctattg cagtaaaagc aaagggcaat     2280

cagctcaagc caaaaacgac tacatcaacc gcaatgataa atattcaaag cggttagatg     2340

atttacagtt ttcaggctat ggtaatatgc caaaatttgc cgaagataat ccgcaagaat     2400

tttggcgatt gtcagatatt tacgagcgag ctaatgcccg agtttgtact gaaattgaat     2460

ttgctttacc tagagaatta accctagaac aacagcaaaa attagtaagt tcgtttatag     2520

aaaatacggt tgatagcggt agcaataaac taccctactc tttcgctatc cataccgata     2580

aaaataatca taatccccat tgtcatttga tattttcaga acgccaactt gacggcatag     2640

accgtacagc cgagcagttt tttaaacgtg ctaatactaa atccccagaa aagggcggag     2700

cgatgaaaac ggcagatttt cgagatcgtg agtttatcca atctgtccga aaaacgtgga     2760

gagagcaagc taatcaagcc ttagagcaat acggatatgc cgcacgaatt gacgaacgta     2820

gctacaagga acaaggcata gagcaagccc caagagcaag aattgacagg gtaacgtggc     2880

aagaattgaa ccgattagag caagaagaac gccaaatcgt gcaagagctt gcacttaaag     2940

gacaagaaat taacaaagaa aaatcctact tgcagaaaat cgaagaaaaa caggctcaag     3000

gaatgggcaa atatgaatcc aaattcgcag ctgcgttttc taaattatcg gaaagtgccc     3060

taaaacacga tttaagcaac gaaaaagaaa aagacagtaa aatacacact caagaagaaa     3120

aagtgcctca aaatcgcatt caggggcttt ctcaagcaga ttttgatcag tttttaattg     3180

atgaatggct acctcaaata gaaaaatacg ttaaagccca agaaaagcgg gacggaatgg     3240

aagtagagat cacgcaatac gacaaggatt tacagcgtat tcagggagac tataacaagc     3300

tcacagataa aaatcagggt tttctcggtt tatgggaaac taaagagcaa aaagcaaaga     3360

aaaaagagct tgaagatgaa tacaaacata cagcagagca acggaacgct aaaagccaag     3420

aattagccga gtatagccaa aaaataaaag catacgaaca gaaaacgcta gagccaatca     3480

acgagaagat tgccaaatat caagctgaca accctgaaat aaaaatgcgg agcttaggat     3540

ttgtgaaaaa aattaaggct caaggggcat ataaagcggc tcaagagcga atggagcgag     3600

aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt ggtttgagct     3660

tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa ctcgcaacaa     3720

gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct gcccagcggt     3780

tgaaaattta cctattcacc attacaatga tcaagcagga aatttttttg attgccgtaa     3840

atgtccgtat atctagttga ggcacaaccc gccaaagtca ttgccccaac cagaacggcg     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggctc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa     4200

aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt     4260

atcagatatt tgttctgaaa tgaagacgta tcgggtgttt gcccgatgtt tttaggtttt     4320

tatcaaattt acaaaaggaa gcccatatga aaaccttctt caaaaccctt tccgccgccg     4380

cactcgcgct catcctcgca gcctgcggcg gtcaaaaaga cagcgcgccc gcagcctctg     4440

ccgccgcccc ttctgccgat aacggcgcgg cgaaaaaaga aatcgtcttc ggcacgaccg     4500

tcggcgactt cggcgatatg gtcaaagaac aaatccaagc cgagctggag aaaaaaggct     4560

acaccgtcaa attggtcgaa tttaccgact atgtgcgccc gaatctggca ttggcggagg     4620

gcgagttgga catcaacgtc ttccaacaca aaccctatct tgacgatttc aaaaaagaac     4680

acaacctgga catcaccgaa gccttccaag tgccgaccgc gcctttggga ctgtatccgg     4740

gcaaactgaa atcgctggaa gaagtcaaag acggcagcac cgtatccgcg cccaacgacc     4800

cgtccaactt cgcacgcgcc ttggtgatgc tgaacgaact gggttggatc aaactcaaag     4860

acggcatcaa tccgctgacc gcatccaaag ccgacatcgc ggaaaacctg aaaaacatca     4920

aaatcgtcga gcttgaagcc gcacaactgc cgcgcagccg cgccgacgtg gattttgccg     4980

tcgtcaacgg caactacgcc ataagcagcg gcatgaagct gaccgaagcc ctgttccaag     5040

agccgagctt tgcctatgtc aactggtctg ccgtcaaaac cgccgacaaa gacagccaat     5100

ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt caaagcctac gcgcacaaac     5160

gcttcgaggg ctacaaatac cctgccgcat ggaatgaagg cgcagccaaa taagtgcggt     5220

catcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg     5280

aagttgcccc aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc     5340

aacgccatga gcggcctcat ttcttattct gagttacaac agtccgcacc gctgtccggt     5400

agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt     5460

atcatgcaac tcgtaggaca ggtgccggca gcgcccaaca gtcccccggc cacggggcct     5520

gccaccatac ccacgccgaa acaagcgccc tgcaccatta tgttccggat ctgcatcgca     5580

ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt     5640

ttatcaggct ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga     5700

gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag     5760

tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac     5820

cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat     5880

tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg     5940

ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca     6000

tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta     6060

taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa     6120

tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac     6180

cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt     6240

agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaacgtt tcagtttgct     6300

catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca     6360

ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg     6420

gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa     6480

cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat     6540

gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct     6600

tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat     6660

ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc     6720

agggcttccc ggtatcaaca                                                 6740


<210>  34
<211>  6740
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-MetQSM plasmid

<400>  34
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcat aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccaat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cttataacag gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt tttttcagga tttaaaatta caaaaccctt acagagcaag     1440

taaacttgtt tgcttgttct gcaagggttc agcaaccgta gccgtcaggc gtagggcggt     1500

agcctataaa agccatttaa ttttatcttt aaacttcctt ttaaatgctt tgagtgggtg     1560

tcttttatcg tactcatcaa tccttttttg cattctttcg tttgctttgt gatcggcaaa     1620

ttttgaataa gatttttcca tctcatctaa cattctatca atccgttttt tatgttgcca     1680

tttcaggtaa acataaacac ttatagcaat taaagacaat atcaatacat tgtaaaaaat     1740

gattgttaca atttcgctca cagttatttt ttaccttttt caatttcttc attgataaat     1800

gcactcaatt catcaaattt cttgtcatca ttgataaatt tacgcaactt agggaagttt     1860

ctatctacat ctaaaagagg gttatttatt atttcattta gccaaaaagc ccctaataaa     1920

accttgtaat gcgtagcttt cttacgcttt tctgcttgtt cttttgactt aatcgcacga     1980

attttcgctt tgatttcgtc ctgcttgcgt tgtaaatctg cttgttgctg ttccaatctt     2040

gtaagttttt cgcttgccat actagcccct ttatatagtt agaaattatc gttattttat     2100

tcagtaggtg ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac     2160

ttatcagttt ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac     2220

tcaacactat ggcaatctat catttaaacg ttcgctattg cagtaaaagc aaagggcaat     2280

cagctcaagc caaaaacgac tacatcaacc gcaatgataa atattcaaag cggttagatg     2340

atttacagtt ttcaggctat ggtaatatgc caaaatttgc cgaagataat ccgcaagaat     2400

tttggcgatt gtcagatatt tacgagcgag ctaatgcccg agtttgtact gaaattgaat     2460

ttgctttacc tagagaatta accctagaac aacagcaaaa attagtaagt tcgtttatag     2520

aaaatacggt tgatagcggt agcaataaac taccctactc tttcgctatc cataccgata     2580

aaaataatca taatccccat tgtcatttga tattttcaga acgccaactt gacggcatag     2640

accgtacagc cgagcagttt tttaaacgtg ctaatactaa atccccagaa aagggcggag     2700

cgatgaaaac ggcagatttt cgagatcgtg agtttatcca atctgtccga aaaacgtgga     2760

gagagcaagc taatcaagcc ttagagcaat acggatatgc cgcacgaatt gacgaacgta     2820

gctacaagga acaaggcata gagcaagccc caagagcaag aattgacagg gtaacgtggc     2880

aagaattgaa ccgattagag caagaagaac gccaaatcgt gcaagagctt gcacttaaag     2940

gacaagaaat taacaaagaa aaatcctact tgcagaaaat cgaagaaaaa caggctcaag     3000

gaatgggcaa atatgaatcc aaattcgcag ctgcgttttc taaattatcg gaaagtgccc     3060

taaaacacga tttaagcaac gaaaaagaaa aagacagtaa aatacacact caagaagaaa     3120

aagtgcctca aaatcgcatt caggggcttt ctcaagcaga ttttgatcag tttttaattg     3180

atgaatggct acctcaaata gaaaaatacg ttaaagccca agaaaagcgg gacggaatgg     3240

aagtagagat cacgcaatac gacaaggatt tacagcgtat tcagggagac tataacaagc     3300

tcacagataa aaatcagggt tttctcggtt tatgggaaac taaagagcaa aaagcaaaga     3360

aaaaagagct tgaagatgaa tacaaacata cagcagagca acggaacgct aaaagccaag     3420

aattagccga gtatagccaa aaaataaaag catacgaaca gaaaacgcta gagccaatca     3480

acgagaagat tgccaaatat caagctgaca accctgaaat aaaaatgcgg agcttaggat     3540

ttgtgaaaaa aattaaggct caaggggcat ataaagcggc tcaagagcga atggagcgag     3600

aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt ggtttgagct     3660

tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa ctcgcaacaa     3720

gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct gcccagcggt     3780

tgaaaattta cctattcacc attacaatga tcaagcagga aatttttttg attgccgtaa     3840

atgtccgtat atctagttga ggcacaaccc gccaaagtca ttgccccaac cagaacggcg     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggctc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa     4200

aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt     4260

atcagatatt tgttctgaaa tgaagacgta tcgggtgttt gcccgatgtt tttaggtttt     4320

tatcaaattt acaaaaggaa gcccatatga aaaccttctt caaaaccctt tccgccgccg     4380

cactcgcgct catcctcgca gcctgcggcg gtcaaaaaga cagcgcgccc gcagcctctg     4440

ccgccgcccc ttctgccgat aacggcgcgg cgaaaaaaga aatcgtcttc ggcacgaccg     4500

tcggcgactt cggcgatatg gtcaaagaac aaatccaagc cgagctggag aaaaaaggct     4560

acaccgtcaa attggtcgaa tttaccgact atgtgcgccc gaatctggca ttggcggagg     4620

gcgagttgga catcaacgtc ttccaacaca aaccctatct tgacgatttc aaaaaagaac     4680

acaacctgga catcaccgaa gccttccaag tgccgaccgc gcctttggga ctgtatccgg     4740

gcaaactgaa atcgctggaa gaagtcaaag acggcagcac cgtatccgcg cccaacgacc     4800

cgtccaactt cgcacgcgcc ttggtgatgc tgaacgaact gggttggatc aaactcaaag     4860

acggcatcaa tccgctgacc gcatccaaag ccgacatcgc ggaaaacctg aaaaacatca     4920

aaatcgtcga gcttgaagcc gcacaactgc cgcgcagccg cgccgacgtg gattttgccg     4980

tcgtcaacgg caactacgcc ataagcagcg gcatgaagct gaccgaagcc ctgttccaag     5040

agccgagctt tgcctatgtc gcctggtctg ccgtcaaaac cgccgacaaa gacagccaat     5100

ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt caaagcctac gcgcacaaac     5160

gcttcgaggg ctacaaatac cctgccgcat ggaatgaagg cgcagccaaa taagtgcggt     5220

catcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg     5280

aagttgcccc aacagagagt ggaaccaacc ggtgatacca cgatactatg actgagagtc     5340

aacgccatga gcggcctcat ttcttattct gagttacaac agtccgcacc gctgtccggt     5400

agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt     5460

atcatgcaac tcgtaggaca ggtgccggca gcgcccaaca gtcccccggc cacggggcct     5520

gccaccatac ccacgccgaa acaagcgccc tgcaccatta tgttccggat ctgcatcgca     5580

ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg ctaaccgttt     5640

ttatcaggct ctgggaggca gaataaatga tcatatcgtc aattattacc tccacgggga     5700

gagcctgagc aaactggcct caggcatttg agaagcacac ggtcacactg cttccggtag     5760

tcaataaacc ggtaaaccag caatagacat aagcggctat ttaacgaccc tgccctgaac     5820

cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc atccgcttat tatcacttat     5880

tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg     5940

ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca     6000

tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta     6060

taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa     6120

tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac     6180

cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt     6240

agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaacgtt tcagtttgct     6300

catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca ccgtctttca     6360

ttgccatacg gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg     6420

gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa     6480

cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat     6540

gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct     6600

tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat     6660

ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc     6720

agggcttccc ggtatcaaca                                                 6740


<210>  35
<211>  6821
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-GNA1220 plasmid

<400>  35
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcat aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccaat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cttataacag gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt tttttcagga tttaaaatta caaaaccctt acagagcaag     1440

taaacttgtt tgcttgttct gcaagggttc agcaaccgta gccgtcaggc gtagggcggt     1500

agcctataaa agccatttaa ttttatcttt aaacttcctt ttaaatgctt tgagtgggtg     1560

tcttttatcg tactcatcaa tccttttttg cattctttcg tttgctttgt gatcggcaaa     1620

ttttgaataa gatttttcca tctcatctaa cattctatca atccgttttt tatgttgcca     1680

tttcaggtaa acataaacac ttatagcaat taaagacaat atcaatacat tgtaaaaaat     1740

gattgttaca atttcgctca cagttatttt ttaccttttt caatttcttc attgataaat     1800

gcactcaatt catcaaattt cttgtcatca ttgataaatt tacgcaactt agggaagttt     1860

ctatctacat ctaaaagagg gttatttatt atttcattta gccaaaaagc ccctaataaa     1920

accttgtaat gcgtagcttt cttacgcttt tctgcttgtt cttttgactt aatcgcacga     1980

attttcgctt tgatttcgtc ctgcttgcgt tgtaaatctg cttgttgctg ttccaatctt     2040

gtaagttttt cgcttgccat actagcccct ttatatagtt agaaattatc gttattttat     2100

tcagtaggtg ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac     2160

ttatcagttt ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac     2220

tcaacactat ggcaatctat catttaaacg ttcgctattg cagtaaaagc aaagggcaat     2280

cagctcaagc caaaaacgac tacatcaacc gcaatgataa atattcaaag cggttagatg     2340

atttacagtt ttcaggctat ggtaatatgc caaaatttgc cgaagataat ccgcaagaat     2400

tttggcgatt gtcagatatt tacgagcgag ctaatgcccg agtttgtact gaaattgaat     2460

ttgctttacc tagagaatta accctagaac aacagcaaaa attagtaagt tcgtttatag     2520

aaaatacggt tgatagcggt agcaataaac taccctactc tttcgctatc cataccgata     2580

aaaataatca taatccccat tgtcatttga tattttcaga acgccaactt gacggcatag     2640

accgtacagc cgagcagttt tttaaacgtg ctaatactaa atccccagaa aagggcggag     2700

cgatgaaaac ggcagatttt cgagatcgtg agtttatcca atctgtccga aaaacgtgga     2760

gagagcaagc taatcaagcc ttagagcaat acggatatgc cgcacgaatt gacgaacgta     2820

gctacaagga acaaggcata gagcaagccc caagagcaag aattgacagg gtaacgtggc     2880

aagaattgaa ccgattagag caagaagaac gccaaatcgt gcaagagctt gcacttaaag     2940

gacaagaaat taacaaagaa aaatcctact tgcagaaaat cgaagaaaaa caggctcaag     3000

gaatgggcaa atatgaatcc aaattcgcag ctgcgttttc taaattatcg gaaagtgccc     3060

taaaacacga tttaagcaac gaaaaagaaa aagacagtaa aatacacact caagaagaaa     3120

aagtgcctca aaatcgcatt caggggcttt ctcaagcaga ttttgatcag tttttaattg     3180

atgaatggct acctcaaata gaaaaatacg ttaaagccca agaaaagcgg gacggaatgg     3240

aagtagagat cacgcaatac gacaaggatt tacagcgtat tcagggagac tataacaagc     3300

tcacagataa aaatcagggt tttctcggtt tatgggaaac taaagagcaa aaagcaaaga     3360

aaaaagagct tgaagatgaa tacaaacata cagcagagca acggaacgct aaaagccaag     3420

aattagccga gtatagccaa aaaataaaag catacgaaca gaaaacgcta gagccaatca     3480

acgagaagat tgccaaatat caagctgaca accctgaaat aaaaatgcgg agcttaggat     3540

ttgtgaaaaa aattaaggct caaggggcat ataaagcggc tcaagagcga atggagcgag     3600

aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt ggtttgagct     3660

tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa ctcgcaacaa     3720

gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct gcccagcggt     3780

tgaaaattta cctattcacc attacaatga tcaagcagga aatttttttg attgccgtaa     3840

atgtccgtat atctagttga ggcacaaccc gccaaagtca ttgccccaac cagaacggcg     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggctc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa     4200

aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt     4260

atcagatatt tgttctgaaa tgaagacgta tcgggtgttt gcccgatgtt tttaggtttt     4320

tatcaaattt acaaaaggaa gcccatatgg aatttttcat tatcttgttg gcagccgtcg     4380

ccgttttcgg cttcaaatcc tttgtcgtca tcccccagca ggaagtccac gttgtcgaaa     4440

ggctcgggcg tttccatcgc gccctgacgg ccggtttgaa tattttgatt ccctttatcg     4500

accgcgtcgc ctaccgccat tcgctgaaag aaatcccttt agacgtaccc agccaggtct     4560

gcatcacgcg cgataatacg caattgactg ttgacggcat catctatttc caagtaaccg     4620

atcccaaact cgcctcatac ggttcgagca actacattat ggcaattacc cagcttgccc     4680

aaacgacgct gcgttccgtt atcgggcgta tggagttgga caaaacgttt gaagaacgcg     4740

acgaaatcaa cagtaccgtc gtctccgccc tcgatgaagc cgccggggct tggggtgtga     4800

aagtcctccg ttacgaaatc aaggatttgg ttccgccgca agaaatcctt cgcgcaatgc     4860

aggcacaaat taccgccgaa cgcgaaaaac gcgcccgtat tgccgaatcc gaaggccgta     4920

aaatcgaaca aatcaacctt gccagtggtc agcgtgaagc cgaaatccaa caatccgaag     4980

gcgaggctca ggctgcggtc aatgcgtcca atgccgagaa aatcgcccgc atcaaccgcg     5040

ccaaaggcga agcggaatcc ctgcgccttg ttgccgaagc caatgccgaa gccatccgtc     5100

aaattgccgc cgcccttcaa acccaaggcg gggcggatgc ggtcaatctg aagattgcgg     5160

aacaatacgt agccgcgttc aacaatcttg ccaaagaaag caatacgctg attatgcccg     5220

ccaatgttgc cgacatcggc agcctgattt ctgccggcat gaaaattatc gacagcagca     5280

aaaccgccaa ataagtgcgg tcatcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg     5340

gaagtcccct acgtgctgct gaagttgccc caacagagag tggaaccaac cggtgatacc     5400

acgatactat gactgagagt caacgccatg agcggcctca tttcttattc tgagttacaa     5460

cagtccgcac cgctgtccgg tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc     5520

gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac     5580

agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgcc ctgcaccatt     5640

atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta     5700

ttaacgaagc gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt     5760

caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca     5820

cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta     5880

tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt     5940

catccgctta ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg     6000

ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc     6060

attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc     6120

agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg     6180

tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg     6240

aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc     6300

acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc     6360

gatgaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata     6420

tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg     6480

caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa     6540

aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg     6600

cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt     6660

ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg     6720

gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgccga tcaacgtctc     6780

attttcgcca aaagttggcc cagggcttcc cggtatcaac a                         6821


<210>  36
<211>  6374
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-GNA1220_helix-bab plasmid

<400>  36
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcat aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccaat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cttataacag gaaatttgtt gtcgtataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt tttttcagga tttaaaatta caaaaccctt acagagcaag     1440

taaacttgtt tgcttgttct gcaagggttc agcaaccgta gccgtcaggc gtagggcggt     1500

agcctataaa agccatttaa ttttatcttt aaacttcctt ttaaatgctt tgagtgggtg     1560

tcttttatcg tactcatcaa tccttttttg cattctttcg tttgctttgt gatcggcaaa     1620

ttttgaataa gatttttcca tctcatctaa cattctatca atccgttttt tatgttgcca     1680

tttcaggtaa acataaacac ttatagcaat taaagacaat atcaatacat tgtaaaaaat     1740

gattgttaca atttcgctca cagttatttt ttaccttttt caatttcttc attgataaat     1800

gcactcaatt catcaaattt cttgtcatca ttgataaatt tacgcaactt agggaagttt     1860

ctatctacat ctaaaagagg gttatttatt atttcattta gccaaaaagc ccctaataaa     1920

accttgtaat gcgtagcttt cttacgcttt tctgcttgtt cttttgactt aatcgcacga     1980

attttcgctt tgatttcgtc ctgcttgcgt tgtaaatctg cttgttgctg ttccaatctt     2040

gtaagttttt cgcttgccat actagcccct ttatatagtt agaaattatc gttattttat     2100

tcagtaggtg ctaggcttgc aagtgttctg ttcattacgt taaaataacg taatgcccac     2160

ttatcagttt ctcttcgaga aactggtggg caagcgtacc gcttgaccgt ttcgcaatac     2220

tcaacactat ggcaatctat catttaaacg ttcgctattg cagtaaaagc aaagggcaat     2280

cagctcaagc caaaaacgac tacatcaacc gcaatgataa atattcaaag cggttagatg     2340

atttacagtt ttcaggctat ggtaatatgc caaaatttgc cgaagataat ccgcaagaat     2400

tttggcgatt gtcagatatt tacgagcgag ctaatgcccg agtttgtact gaaattgaat     2460

ttgctttacc tagagaatta accctagaac aacagcaaaa attagtaagt tcgtttatag     2520

aaaatacggt tgatagcggt agcaataaac taccctactc tttcgctatc cataccgata     2580

aaaataatca taatccccat tgtcatttga tattttcaga acgccaactt gacggcatag     2640

accgtacagc cgagcagttt tttaaacgtg ctaatactaa atccccagaa aagggcggag     2700

cgatgaaaac ggcagatttt cgagatcgtg agtttatcca atctgtccga aaaacgtgga     2760

gagagcaagc taatcaagcc ttagagcaat acggatatgc cgcacgaatt gacgaacgta     2820

gctacaagga acaaggcata gagcaagccc caagagcaag aattgacagg gtaacgtggc     2880

aagaattgaa ccgattagag caagaagaac gccaaatcgt gcaagagctt gcacttaaag     2940

gacaagaaat taacaaagaa aaatcctact tgcagaaaat cgaagaaaaa caggctcaag     3000

gaatgggcaa atatgaatcc aaattcgcag ctgcgttttc taaattatcg gaaagtgccc     3060

taaaacacga tttaagcaac gaaaaagaaa aagacagtaa aatacacact caagaagaaa     3120

aagtgcctca aaatcgcatt caggggcttt ctcaagcaga ttttgatcag tttttaattg     3180

atgaatggct acctcaaata gaaaaatacg ttaaagccca agaaaagcgg gacggaatgg     3240

aagtagagat cacgcaatac gacaaggatt tacagcgtat tcagggagac tataacaagc     3300

tcacagataa aaatcagggt tttctcggtt tatgggaaac taaagagcaa aaagcaaaga     3360

aaaaagagct tgaagatgaa tacaaacata cagcagagca acggaacgct aaaagccaag     3420

aattagccga gtatagccaa aaaataaaag catacgaaca gaaaacgcta gagccaatca     3480

acgagaagat tgccaaatat caagctgaca accctgaaat aaaaatgcgg agcttaggat     3540

ttgtgaaaaa aattaaggct caaggggcat ataaagcggc tcaagagcga atggagcgag     3600

aaaaacagca ccaacaggaa aaacaacaga gacatttaga gcgagagagt ggtttgagct     3660

tgtagctaac gccctacgcc tacggcttcg gttgttcaac ccttaaagaa ctcgcaacaa     3720

gttgcaaatt ctttaagggt tcgcaataaa aacaaccgct aaacatttct gcccagcggt     3780

tgaaaattta cctattcacc attacaatga tcaagcagga aatttttttg attgccgtaa     3840

atgtccgtat atctagttga ggcacaaccc gccaaagtca ttgccccaac cagaacggcg     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggctc tgcgcttgaa ttgtgttgta gaaacacaac gtttttgaaa     4200

aaataagcta ttgttttata tcaaaatata atcattttta aaataaaggt tgcggcattt     4260

atcagatatt tgttctgaaa tgaagacgta tcgggtgttt gcccgatgtt tttaggtttt     4320

tatcaaattt acaaaaggaa gcccatatgg tgaaccgaac tgccttctgc ctttctctga     4380

ccgccgccct gattctgacc gcctgcagca gcggaggggg tggtgaacaa atcaaccttg     4440

ccagtggtca gcgtgaagcc gaaatccaac aatccgaagg cgaggctcag gctgcggtca     4500

atgcgtccaa tgccgagaaa atcgcccgca tcaaccgcgc caaaggcgaa gcggaatccc     4560

tgcgccttgt tgccgaagcc aatgccgaag ccatccgtca aattgccgcc gcccttcaaa     4620

cccaaggcgg ggcggatgcg gtcaatctga agattgcgga acaatacgta gccgcgttca     4680

acaatcttgc caaagaaagc aatacgctga ttatgcccgc caatgttgcc gacatcggca     4740

gcctgatttc tgccggcatg aaaattatcg acagcagcaa aaccgccaaa taaaggccta     4800

ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgattaagtg cggtcatctt     4860

cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct gctgaagttg     4920

ccccaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc     4980

atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgtc cggtagctcc     5040

ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg     5100

caactcgtag gacaggtgcc ggcagcgccc aacagtcccc cggccacggg gcctgccacc     5160

atacccacgc cgaaacaagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc     5220

tgctggctac cctgtggaac acctacatct gtattaacga agcgctaacc gtttttatca     5280

ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg gggagagcct     5340

gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg gtagtcaata     5400

aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct gaaccgacga     5460

ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac ttattcaggc     5520

gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc     5580

cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacag     5640

acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat     5700

ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa     5760

ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta     5820

gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac     5880

tgccggaaat cgtcgtggta ttcactccag agcgatgaaa cgtttcagtt tgctcatgga     5940

aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca     6000

tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa     6060

acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct     6120

ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt     6180

gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc     6240

ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa     6300

agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct     6360

tcccggtatc aaca                                                       6374


<210>  37
<211>  426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Neisseria gonorrhoeae NHBA protein

<400>  37

Met Phe Lys Arg Ser Val Ile Ala Met Ala Cys Ile Phe Pro Leu Ser 
1               5                   10                  15      


Ala Cys Gly Gly Gly Gly Gly Gly Ser Pro Asp Val Lys Ser Ala Asp 
            20                  25                  30          


Thr Pro Ser Lys Pro Ala Ala Pro Val Val Ala Glu Asn Ala Gly Glu 
        35                  40                  45              


Gly Val Leu Pro Lys Glu Lys Lys Asp Glu Glu Ala Ala Gly Gly Ala 
    50                  55                  60                  


Pro Gln Ala Asp Thr Gln Asp Ala Thr Ala Gly Glu Gly Ser Gln Asp 
65                  70                  75                  80  


Met Ala Ala Val Ser Ala Glu Asn Thr Gly Asn Gly Gly Ala Ala Thr 
                85                  90                  95      


Thr Asp Asn Pro Lys Asn Glu Asp Ala Gly Ala Gln Asn Asp Met Pro 
            100                 105                 110         


Gln Asn Ala Ala Glu Ser Ala Asn Gln Thr Gly Asn Asn Gln Pro Ala 
        115                 120                 125             


Gly Ser Ser Asp Ser Ala Pro Ala Ser Asn Pro Ala Pro Ala Asn Gly 
    130                 135                 140                 


Gly Ser Asp Phe Gly Arg Thr Asn Val Gly Asn Ser Val Val Ile Asp 
145                 150                 155                 160 


Gly Pro Ser Gln Asn Ile Thr Leu Thr His Cys Lys Gly Asp Pro Cys 
                165                 170                 175     


Asn Gly Asp Asn Leu Leu Asp Glu Glu Ala Pro Pro Lys Ser Glu Phe 
            180                 185                 190         


Glu Ser Leu Ser Asp Glu Glu Lys Ile Lys Lys Tyr Lys Lys Asp Gly 
        195                 200                 205             


Glu Lys Phe Thr Gly Leu Val Ala Ile Lys Val Glu Asn Asn Gly Leu 
    210                 215                 220                 


Asn Lys Tyr Thr Ile Ile Tyr Gln Ala Gln Pro Thr Arg Ser Ala Arg 
225                 230                 235                 240 


Ser Arg Arg Ser Leu Pro Ala Glu Ile Pro Leu Ile Pro Val Asn Gln 
                245                 250                 255     


Ala Asp Thr Leu Ile Val Asp Gly Glu Ala Val Ser Leu Thr Gly His 
            260                 265                 270         


Ser Gly Asn Ile Phe Ala Pro Glu Gly Asn Tyr Arg Tyr Leu Thr Tyr 
        275                 280                 285             


Gly Ala Glu Lys Leu Pro Gly Gly Ser Tyr Ala Leu Arg Val Gln Gly 
    290                 295                 300                 


Glu Pro Ala Lys Gly Glu Met Leu Ala Gly Thr Ala Val Tyr Asn Gly 
305                 310                 315                 320 


Glu Val Leu His Phe His Met Glu Asn Gly Arg Pro Tyr Pro Ser Gly 
                325                 330                 335     


Gly Arg Phe Ala Ala Lys Val Asp Phe Gly Ser Lys Ser Val Asp Gly 
            340                 345                 350         


Ile Ile Asp Ser Ser Asp Asp Leu His Met Gly Thr Gln Lys Phe Lys 
        355                 360                 365             


Ala Ala Ile Asp Gly Asn Gly Phe Lys Gly Thr Trp Thr Glu Asn Gly 
    370                 375                 380                 


Gly Gly Asp Val Ser Gly Arg Phe Tyr Gly Pro Ala Gly Glu Glu Val 
385                 390                 395                 400 


Ala Gly Lys Tyr Ser Tyr Arg Pro Thr Asp Ala Glu Lys Gly Gly Phe 
                405                 410                 415     


Gly Val Phe Ala Gly Lys Lys Asp Arg Asp 
            420                 425     


<210>  38
<211>  1281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-NHBA plasmid

<400>  38
atgtttaaac gcagtgtgat tgcaatggct tgtatttttc ccctttcagc ctgtgggggc       60

ggcggtggcg gatcgcccga tgtcaagtcg gcggacacgc cgtcaaaacc ggccgccccc      120

gttgttgctg aaaatgccgg ggaaggggtg ctgccgaaag aaaagaaaga tgaggaggca      180

gcgggcggtg cgccgcaagc cgatacgcag gacgcaaccg ccggagaagg cagccaagat      240

atggcggcag tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc      300

aaaaatgaag acgcgggggc gcaaaatgat atgccgcaaa atgccgccga atccgcaaat      360

caaacaggga acaaccaacc cgccggttct tcagattccg cccccgcgtc aaaccctgcc      420

cctgcgaatg gcggtagcga ttttggaagg acgaacgtgg gcaattctgt tgtgattgac      480

ggaccgtcgc aaaatataac gttgacccac tgtaaaggcg atccttgtaa tggtgataat      540

ttattggatg aagaagcacc gccaaaatca gaatttgaat cgttaagtga tgaagagaaa      600

attaagaaat ataaaaaaga tggagaaaag tttactggtt tggttgctat aaaagttgaa      660

aataatgggt tgaataaata taccattatt tatcaagctc agcctactcg ttctgcacgg      720

tcgaggaggt cgcttccggc cgagattccg ctgattcccg tcaatcaggc cgatacgctg      780

attgtggatg gggaagcggt cagcctgacg gggcattccg gcaatatctt cgcgcccgaa      840

gggaattacc ggtatctgac ttacggggcg gaaaaattgc ccggcggatc gtatgccctc      900

cgtgtgcaag gcgaaccggc aaaaggcgaa atgcttgcgg gcacggccgt gtacaacggc      960

gaagtgctgc atttccacat ggaaaacggc cgtccgtacc cgtccggagg caggtttgcc     1020

gcaaaagtcg atttcggcag caaatctgtg gacggcatta tcgacagcag cgatgatttg     1080

cacatgggta cgcaaaaatt caaagccgcc atcgatggaa acggctttaa ggggacttgg     1140

acggaaaatg gcggcgggga tgtttccgga aggttttacg gcccggccgg cgaggaagtg     1200

gcgggaaaat acagctatcg cccgacagat gctgaaaagg gcggattcgg cgtgtttgcc     1260

ggcaaaaaag atcgggattg a                                               1281


<210>  39
<211>  8066
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-NHBA plasmid

<400>  39
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atgtttaaac     4380

gcagtgtgat tgcaatggct tgtatttttc ccctttcagc ctgtgggggc ggcggtggcg     4440

gatcgcccga tgtcaagtcg gcggacacgc cgtcaaaacc ggccgccccc gttgttgctg     4500

aaaatgccgg ggaaggggtg ctgccgaaag aaaagaaaga tgaggaggca gcgggcggtg     4560

cgccgcaagc cgatacgcag gacgcaaccg ccggagaagg cagccaagat atggcggcag     4620

tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc aaaaatgaag     4680

acgcgggggc gcaaaatgat atgccgcaaa atgccgccga atccgcaaat caaacaggga     4740

acaaccaacc cgccggttct tcagattccg cccccgcgtc aaaccctgcc cctgcgaatg     4800

gcggtagcga ttttggaagg acgaacgtgg gcaattctgt tgtgattgac ggaccgtcgc     4860

aaaatataac gttgacccac tgtaaaggcg atccttgtaa tggtgataat ttattggatg     4920

aagaagcacc gccaaaatca gaatttgaat cgttaagtga tgaagagaaa attaagaaat     4980

ataaaaaaga tggagaaaag tttactggtt tggttgctat aaaagttgaa aataatgggt     5040

tgaataaata taccattatt tatcaagctc agcctactcg ttctgcacgg tcgaggaggt     5100

cgcttccggc cgagattccg ctgattcccg tcaatcaggc cgatacgctg attgtggatg     5160

gggaagcggt cagcctgacg gggcattccg gcaatatctt cgcgcccgaa gggaattacc     5220

ggtatctgac ttacggggcg gaaaaattgc ccggcggatc gtatgccctc cgtgtgcaag     5280

gcgaaccggc aaaaggcgaa atgcttgcgg gcacggccgt gtacaacggc gaagtgctgc     5340

atttccacat ggaaaacggc cgtccgtacc cgtccggagg caggtttgcc gcaaaagtcg     5400

atttcggcag caaatctgtg gacggcatta tcgacagcag cgatgatttg cacatgggta     5460

cgcaaaaatt caaagccgcc atcgatggaa acggctttaa ggggacttgg acggaaaatg     5520

gcggcgggga tgtttccgga aggttttacg gcccggccgg cgaggaagtg gcgggaaaat     5580

acagctatcg cccgacagat gctgaaaagg gcggattcgg cgtgtttgcc ggcaaaaaag     5640

atcgggattg aaggcctcct gcaggactag tcggccgtac ctgtcagacc aagtttactc     5700

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     5760

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     5820

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     5880

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     5940

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct     6000

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     6060

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     6120

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     6180

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     6240

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     6300

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     6360

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     6420

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     6480

ctggcctttt gctcacatgt gggccctttc gtctcgcgcg tttcggtgat agcttaagtg     6540

cggtcatctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct     6600

gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata ctatgactga     6660

gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc gcaccgctgc     6720

cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt     6780

ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc cggccacggg     6840

gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc ggatctgcat     6900

cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctaacc     6960

gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg     7020

gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg     7080

gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct     7140

gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac     7200

ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg     7260

ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga     7320

agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt     7380

gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt     7440

ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa     7500

taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata     7560

tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag     7620

tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt     7680

ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa     7740

aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca     7800

gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt     7860

tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag     7920

cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt     7980

cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt     8040

tggcccaggg cttcccggta tcaaca                                          8066


<210>  40
<211>  6266
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-MetQ plasmid

<400>  40
gcatgcatcc gggcagcagc gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta       60

gaaacacaac gtttttgaaa aaataagcta ttgttttata tcaaaatata atcattttta      120

aaataaaggt tgcggcattt atcagatatt tgttctgaaa tgaagacgta tcgggtgttt      180

gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gcccatatga aaaccttctt      240

caaaaccctt tccgccgccg cactcgcgct catcctcgca gcctgcggcg gtcaaaaaga      300

cagcgcgccc gcagcctctg ccgccgcccc ttctgccgat aacggcgcgg cgaaaaaaga      360

aatcgtcttc ggcacgaccg tcggcgactt cggcgatatg gtcaaagaac aaatccaagc      420

cgagctggag aaaaaaggct acaccgtcaa attggtcgaa tttaccgact atgtgcgccc      480

gaatctggca ttggcggagg gcgagttgga catcaacgtc ttccaacaca aaccctatct      540

tgacgatttc aaaaaagaac acaacctgga catcaccgaa gccttccaag tgccgaccgc      600

gcctttggga ctgtatccgg gcaaactgaa atcgctggaa gaagtcaaag acggcagcac      660

cgtatccgcg cccaacgacc cgtccaactt cgcacgcgcc ttggtgatgc tgaacgaact      720

gggttggatc aaactcaaag acggcatcaa tccgctgacc gcatccaaag ccgacatcgc      780

ggaaaacctg aaaaacatca aaatcgtcga gcttgaagcc gcacaactgc cgcgcagccg      840

cgccgacgtg gattttgccg tcgtcaacgg caactacgcc ataagcagcg gcatgaagct      900

gaccgaagcc ctgttccaag agccgagctt tgcctatgtc aactggtctg ccgtcaaaac      960

cgccgacaaa gacagccaat ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt     1020

caaagcctac gcgcacaaac gcttcgaggg ctacaaatac cctgccgcat ggaatgaagg     1080

cgcagccaaa taaggcagtc gtataacctg caggccaagc aaaaccattg tgaaaatgcc     1140

gtccgaacac gataatttac cgttcggacg gcattttgta ttgcaccgtc cgacggcatg     1200

cccaaggggg gaaatcccta ttttcaggcc aaccgctata taatgccgtc tgaaccaacg     1260

agagaatgcc atgcaagctg attttaaccg tcccgtcctg gccgtcgata ccggtacttc     1320

ccgtttgtcg ctcgcgctgc gtgccgacgg cgaaacccgt ctgttccatc aggaagtcgg     1380

cagccgccag tccgaactga ttctgccgga aatccgcacc ctattccgcg atgcaggcat     1440

taccgccgcc gatttgggtg cggtcgtgta cgcacagggt cccggcgcgt ttaccggact     1500

gcgtatcggc atcggtgtag ctcagggttt ggcaacgccg tttgataccc ccttaatcgg     1560

cgtaccctcg ctcgatgccg ccgcctcgct gccgccgccg caaagctgca tccttgccgc     1620

tacggacgct cgtatgggcg aagtgtttta tgcatggttc gatacgctgc tcgagggggg     1680

gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg     1740

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     1800

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     1860

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     1920

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     1980

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     2040

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     2100

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     2160

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     2220

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     2280

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     2340

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     2400

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     2460

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     2520

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     2580

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     2640

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     2700

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     2760

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     2820

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     2880

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     2940

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     3000

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     3060

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     3120

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     3180

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     3240

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     3300

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     3360

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     3420

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     3480

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     3540

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     3600

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     3660

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     3720

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     3780

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     3840

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta     3900

agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac     3960

caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg     4020

agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa     4080

gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt     4140

tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt     4200

agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga     4260

gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc     4320

gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg     4380

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     4440

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     4500

gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc     4560

cgctctagac cagccacggc gcatacaaat tcacccgtcc gcccacaggc gatgtattac     4620

gtatcgaccg catcaaagaa atccaccaag ccctgcccaa tacacacatc gtgatgcacg     4680

gctccagctc cgttccgcaa gaatggctga aagtcatcaa cgaatacggc ggcaatatcg     4740

gcgaaaccta cggcgtgccg gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca     4800

aagtcaacat cgataccgac ttgcgccttg cttctaccgg cgcggtacgc cgctaccttg     4860

ccgaaaatcc gtccgacttt gacccgcgca aatacctgag caaaaccatt gaggccatga     4920

agcaaatctg cctcgaccgt tatcttgcgt ttggctgcga aggtcaggca ggcaaaatca     4980

aacctgtttc gttggaaaaa atggcaagcc gttatgccaa gggcgaattg aaccaaatcg     5040

tcaaataaca ggttgcctgt aaacaaaatg ccgtcccatg ggcaaactta agagtgtgtt     5100

gatagtgcag tatcttaaaa ttttgtgtat aataggaatt gaagttaaat tagatgctaa     5160

aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg taatgtagat     5220

aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa ataaatacgt     5280

tagattaatt cctaccagtg actaatctta tgacttttta aacagataac taaaattaca     5340

aacaaatcgt ttaacttctg tatttattta cagatgtaat cacttcagga gtaattacat     5400

gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat     5460

aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg     5520

gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag     5580

tcatctattc aacttatcgt cagaaaaatt aaaactgaac attcgtgtca ctttaattca     5640

ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat     5700

tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga     5760

catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact     5820

agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg     5880

ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga     5940

tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga     6000

atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa     6060

caatttaagt accattactt atgagcaagt attgtctatt tttaatagtt atctattatt     6120

taacgggagg aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta     6180

aagggaatgg agataaatta ttagatatac tactgacagc ttccaagaag ctaaagaggt     6240

ccctttcgac ggccccgggc ctcagc                                          6266


<210>  41
<211>  6266
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-MetQSM plasmid

<400>  41
gcatgcatcc gggcagcagc gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta       60

gaaacacaac gtttttgaaa aaataagcta ttgttttata tcaaaatata atcattttta      120

aaataaaggt tgcggcattt atcagatatt tgttctgaaa tgaagacgta tcgggtgttt      180

gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gcccatatga aaaccttctt      240

caaaaccctt tccgccgccg cactcgcgct catcctcgca gcctgcggcg gtcaaaaaga      300

cagcgcgccc gcagcctctg ccgccgcccc ttctgccgat aacggcgcgg cgaaaaaaga      360

aatcgtcttc ggcacgaccg tcggcgactt cggcgatatg gtcaaagaac aaatccaagc      420

cgagctggag aaaaaaggct acaccgtcaa attggtcgaa tttaccgact atgtgcgccc      480

gaatctggca ttggcggagg gcgagttgga catcaacgtc ttccaacaca aaccctatct      540

tgacgatttc aaaaaagaac acaacctgga catcaccgaa gccttccaag tgccgaccgc      600

gcctttggga ctgtatccgg gcaaactgaa atcgctggaa gaagtcaaag acggcagcac      660

cgtatccgcg cccaacgacc cgtccaactt cgcacgcgcc ttggtgatgc tgaacgaact      720

gggttggatc aaactcaaag acggcatcaa tccgctgacc gcatccaaag ccgacatcgc      780

ggaaaacctg aaaaacatca aaatcgtcga gcttgaagcc gcacaactgc cgcgcagccg      840

cgccgacgtg gattttgccg tcgtcaacgg caactacgcc ataagcagcg gcatgaagct      900

gaccgaagcc ctgttccaag agccgagctt tgcctatgtc gcctggtctg ccgtcaaaac      960

cgccgacaaa gacagccaat ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt     1020

caaagcctac gcgcacaaac gcttcgaggg ctacaaatac cctgccgcat ggaatgaagg     1080

cgcagccaaa taaggcagtc gtataacctg caggccaagc aaaaccattg tgaaaatgcc     1140

gtccgaacac gataatttac cgttcggacg gcattttgta ttgcaccgtc cgacggcatg     1200

cccaaggggg gaaatcccta ttttcaggcc aaccgctata taatgccgtc tgaaccaacg     1260

agagaatgcc atgcaagctg attttaaccg tcccgtcctg gccgtcgata ccggtacttc     1320

ccgtttgtcg ctcgcgctgc gtgccgacgg cgaaacccgt ctgttccatc aggaagtcgg     1380

cagccgccag tccgaactga ttctgccgga aatccgcacc ctattccgcg atgcaggcat     1440

taccgccgcc gatttgggtg cggtcgtgta cgcacagggt cccggcgcgt ttaccggact     1500

gcgtatcggc atcggtgtag ctcagggttt ggcaacgccg tttgataccc ccttaatcgg     1560

cgtaccctcg ctcgatgccg ccgcctcgct gccgccgccg caaagctgca tccttgccgc     1620

tacggacgct cgtatgggcg aagtgtttta tgcatggttc gatacgctgc tcgagggggg     1680

gcccggtacc agcttttgtt ccctttagtg agggttaatt tcgagcttgg cgtaatcatg     1740

gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc     1800

cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc     1860

gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat     1920

cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac     1980

tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt     2040

aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca     2100

gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc     2160

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact     2220

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct     2280

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag     2340

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca     2400

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa     2460

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc     2520

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag     2580

aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg     2640

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca     2700

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc     2760

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag     2820

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata     2880

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat     2940

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg     3000

ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc     3060

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     3120

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     3180

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     3240

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     3300

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     3360

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     3420

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     3480

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     3540

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     3600

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     3660

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     3720

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     3780

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     3840

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta     3900

agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac     3960

caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg     4020

agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa     4080

gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt     4140

tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt     4200

agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga     4260

gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc     4320

gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg     4380

gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct     4440

gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg     4500

gccagtgaat tgtaatacga ctcactatag ggcgaattgg agctccaccg cggtggcggc     4560

cgctctagac cagccacggc gcatacaaat tcacccgtcc gcccacaggc gatgtattac     4620

gtatcgaccg catcaaagaa atccaccaag ccctgcccaa tacacacatc gtgatgcacg     4680

gctccagctc cgttccgcaa gaatggctga aagtcatcaa cgaatacggc ggcaatatcg     4740

gcgaaaccta cggcgtgccg gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca     4800

aagtcaacat cgataccgac ttgcgccttg cttctaccgg cgcggtacgc cgctaccttg     4860

ccgaaaatcc gtccgacttt gacccgcgca aatacctgag caaaaccatt gaggccatga     4920

agcaaatctg cctcgaccgt tatcttgcgt ttggctgcga aggtcaggca ggcaaaatca     4980

aacctgtttc gttggaaaaa atggcaagcc gttatgccaa gggcgaattg aaccaaatcg     5040

tcaaataaca ggttgcctgt aaacaaaatg ccgtcccatg ggcaaactta agagtgtgtt     5100

gatagtgcag tatcttaaaa ttttgtgtat aataggaatt gaagttaaat tagatgctaa     5160

aaatttgtaa ttaagaagga gggattcgtc atgttggtat tccaaatgcg taatgtagat     5220

aaaacatcta ctgttttgaa acagactaaa aacagtgatt acgcagataa ataaatacgt     5280

tagattaatt cctaccagtg actaatctta tgacttttta aacagataac taaaattaca     5340

aacaaatcgt ttaacttctg tatttattta cagatgtaat cacttcagga gtaattacat     5400

gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat     5460

aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg     5520

gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag     5580

tcatctattc aacttatcgt cagaaaaatt aaaactgaac attcgtgtca ctttaattca     5640

ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat     5700

tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga     5760

catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact     5820

agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg     5880

ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga     5940

tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga     6000

atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa     6060

caatttaagt accattactt atgagcaagt attgtctatt tttaatagtt atctattatt     6120

taacgggagg aaataattct atgagtcgct tttttaaatt tggaaagtta cacgttacta     6180

aagggaatgg agataaatta ttagatatac tactgacagc ttccaagaag ctaaagaggt     6240

ccctttcgac ggccccgggc ctcagc                                          6266


<210>  42
<211>  6350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-GNA1220 plasmid

<400>  42
gcatgcatcc gggcagcagc gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta       60

gaaacacaac gtttttgaaa aaataagcta ttgttttata tcaaaatata atcattttta      120

aaataaaggt tgcggcattt atcagatatt tgttctgaaa tgaagacgta tcgggtgttt      180

gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gcccatatga tggaattttt      240

cattatcttg ttggcagccg tcgccgtttt cggcttcaaa tcctttgtcg tcatccccca      300

gcaggaagtc cacgttgtcg aaaggctcgg gcgtttccat cgcgccctga cggccggttt      360

gaatattttg attcccttta tcgaccgcgt cgcctaccgc cattcgctga aagaaatccc      420

tttagacgta cccagccagg tctgcatcac gcgcgataat acgcaattga ctgttgacgg      480

catcatctat ttccaagtaa ccgatcccaa actcgcctca tacggttcga gcaactacat      540

tatggcaatt acccagcttg cccaaacgac gctgcgttcc gttatcgggc gtatggagtt      600

ggacaaaacg tttgaagaac gcgacgaaat caacagtacc gtcgtctccg ccctcgatga      660

agccgccggg gcttggggtg tgaaagtcct ccgttacgaa atcaaggatt tggttccgcc      720

gcaagaaatc cttcgcgcaa tgcaggcaca aattaccgcc gaacgcgaaa aacgcgcccg      780

tattgccgaa tccgaaggcc gtaaaatcga acaaatcaac cttgccagtg gtcagcgtga      840

agccgaaatc caacaatccg aaggcgaggc tcaggctgcg gtcaatgcgt ccaatgccga      900

gaaaatcgcc cgcatcaacc gcgccaaagg cgaagcggaa tccctgcgcc ttgttgccga      960

agccaatgcc gaagccatcc gtcaaattgc cgccgccctt caaacccaag gcggggcgga     1020

tgcggtcaat ctgaagattg cggaacaata cgtagccgcg ttcaacaatc ttgccaaaga     1080

aagcaatacg ctgattatgc ccgccaatgt tgccgacatc ggcagcctga tttctgccgg     1140

catgaaaatt atcgacagca gcaaaaccgc caaataaggc agtcgtataa cctgcaggcc     1200

aagcaaaacc attgtgaaaa tgccgtccga acacgataat ttaccgttcg gacggcattt     1260

tgtattgcac cgtccgacgg catgcccaag gggggaaatc cctattttca ggccaaccgc     1320

tatataatgc cgtctgaacc aacgagagaa tgccatgcaa gctgatttta accgtcccgt     1380

cctggccgtc gataccggta cttcccgttt gtcgctcgcg ctgcgtgccg acggcgaaac     1440

ccgtctgttc catcaggaag tcggcagccg ccagtccgaa ctgattctgc cggaaatccg     1500

caccctattc cgcgatgcag gcattaccgc cgccgatttg ggtgcggtcg tgtacgcaca     1560

gggtcccggc gcgtttaccg gactgcgtat cggcatcggt gtagctcagg gtttggcaac     1620

gccgtttgat acccccttaa tcggcgtacc ctcgctcgat gccgccgcct cgctgccgcc     1680

gccgcaaagc tgcatccttg ccgctacgga cgctcgtatg ggcgaagtgt tttatgcatg     1740

gttcgatacg ctgctcgagg gggggcccgg taccagcttt tgttcccttt agtgagggtt     1800

aatttcgagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct     1860

cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg     1920

agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct     1980

gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg     2040

gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc     2100

ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg     2160

aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct     2220

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     2280

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     2340

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     2400

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     2460

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     2520

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     2580

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     2640

gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc     2700

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     2760

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     2820

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     2880

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag     2940

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat     3000

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc     3060

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat     3120

accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag     3180

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg     3240

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc     3300

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca     3360

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg     3420

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc     3480

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta     3540

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc     3600

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg     3660

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc     3720

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc     3780

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat     3840

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag     3900

cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc     3960

ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt     4020

tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca     4080

aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta     4140

aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta     4200

cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg     4260

aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga     4320

aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg     4380

ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg     4440

ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc     4500

cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc     4560

cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa     4620

ttggagctcc accgcggtgg cggccgctct agaccagcca cggcgcatac aaattcaccc     4680

gtccgcccac aggcgatgta ttacgtatcg accgcatcaa agaaatccac caagccctgc     4740

ccaatacaca catcgtgatg cacggctcca gctccgttcc gcaagaatgg ctgaaagtca     4800

tcaacgaata cggcggcaat atcggcgaaa cctacggcgt gccggttgaa gaaatcgtcg     4860

aaggcatcaa acacggcgtg cgcaaagtca acatcgatac cgacttgcgc cttgcttcta     4920

ccggcgcggt acgccgctac cttgccgaaa atccgtccga ctttgacccg cgcaaatacc     4980

tgagcaaaac cattgaggcc atgaagcaaa tctgcctcga ccgttatctt gcgtttggct     5040

gcgaaggtca ggcaggcaaa atcaaacctg tttcgttgga aaaaatggca agccgttatg     5100

ccaagggcga attgaaccaa atcgtcaaat aacaggttgc ctgtaaacaa aatgccgtcc     5160

catgggcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt gtataatagg     5220

aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg     5280

gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac taaaaacagt     5340

gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt     5400

tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta tttacagatg     5460

taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa actttttaac     5520

gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt     5580

ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca     5640

ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact     5700

gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca     5760

gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta ttaaaaaagt     5820

ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg     5880

taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca     5940

attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat     6000

aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt     6060

tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca     6120

agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc aagtattgtc     6180

tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt cgctttttta     6240

aatttggaaa gttacacgtt actaaaggga atggagataa attattagat atactactga     6300

cagcttccaa gaagctaaag aggtcccttt cgacggcccc gggcctcagc                6350


<210>  43
<211>  6673
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pBS-FHbpKO-NHba plasmid

<400>  43
gcatgcatcc gggcagcagc gcatcggctc gcacgaggtc tgcgcttgaa ttgtgttgta       60

gaaacacaac gtttttgaaa aaataagcta ttgttttata tcaaaatata atcattttta      120

aaataaaggt tgcggcattt atcagatatt tgttctgaaa tgaagacgta tcgggtgttt      180

gcccgatgtt tttaggtttt tatcaaattt acaaaaggaa gcccatatgt ttaaacgcag      240

tgtgattgca atggcttgta tttttcccct ttcagcctgt gggggcggcg gtggcggatc      300

gcccgatgtc aagtcggcgg acacgccgtc aaaaccggcc gcccccgttg ttgctgaaaa      360

tgccggggaa ggggtgctgc cgaaagaaaa gaaagatgag gaggcagcgg gcggtgcgcc      420

gcaagccgat acgcaggacg caaccgccgg agaaggcagc caagatatgg cggcagtttc      480

ggcagaaaat acaggcaatg gcggtgcggc aacaacggac aaccccaaaa atgaagacgc      540

gggggcgcaa aatgatatgc cgcaaaatgc cgccgaatcc gcaaatcaaa cagggaacaa      600

ccaacccgcc ggttcttcag attccgcccc cgcgtcaaac cctgcccctg cgaatggcgg      660

tagcgatttt ggaaggacga acgtgggcaa ttctgttgtg attgacggac cgtcgcaaaa      720

tataacgttg acccactgta aaggcgatcc ttgtaatggt gataatttat tggatgaaga      780

agcaccgcca aaatcagaat ttgaatcgtt aagtgatgaa gagaaaatta agaaatataa      840

aaaagatgga gaaaagttta ctggtttggt tgctataaaa gttgaaaata atgggttgaa      900

taaatatacc attatttatc aagctcagcc tactcgttct gcacggtcga ggaggtcgct      960

tccggccgag attccgctga ttcccgtcaa tcaggccgat acgctgattg tggatgggga     1020

agcggtcagc ctgacggggc attccggcaa tatcttcgcg cccgaaggga attaccggta     1080

tctgacttac ggggcggaaa aattgcccgg cggatcgtat gccctccgtg tgcaaggcga     1140

accggcaaaa ggcgaaatgc ttgcgggcac ggccgtgtac aacggcgaag tgctgcattt     1200

ccatatggaa aacggccgtc cgtacccgtc cggaggcagg tttgccgcaa aagtcgattt     1260

cggcagcaaa tctgtggacg gcattatcga cagcagcgat gatttgcata tgggtacgca     1320

aaaattcaaa gccgccatcg atggaaacgg ctttaagggg acttggacgg aaaatggcgg     1380

cggggatgtt tccggaaggt tttacggccc ggccggcgag gaagtggcgg gaaaatacag     1440

ctatcgcccg acagatgctg aaaagggcgg attcggcgtg tttgccggca aaaaagatcg     1500

ggattgaagg cctcctgcag gccaagcaaa accattgtga aaatgccgtc cgaacacgat     1560

aatttaccgt tcggacggca ttttgtattg caccgtccga cggcatgccc aaggggggaa     1620

atccctattt tcaggccaac cgctatataa tgccgtctga accaacgaga gaatgccatg     1680

caagctgatt ttaaccgtcc cgtcctggcc gtcgataccg gtacttcccg tttgtcgctc     1740

gcgctgcgtg ccgacggcga aacccgtctg ttccatcagg aagtcggcag ccgccagtcc     1800

gaactgattc tgccggaaat ccgcacccta ttccgcgatg caggcattac cgccgccgat     1860

ttgggtgcgg tcgtgtacgc acagggtccc ggcgcgttta ccggactgcg tatcggcatc     1920

ggtgtagctc agggtttggc aacgccgttt gataccccct taatcggcgt accctcgctc     1980

gatgccgccg cctcgctgcc gccgccgcaa agctgcatcc ttgccgctac ggacgctcgt     2040

atgggcgaag tgttttatgc atggttcgat acgctgctcg agggggggcc cggtaccagc     2100

ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt     2160

cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag     2220

tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg     2280

cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg     2340

gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc     2400

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc     2460

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg     2520

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat     2580

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag     2640

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga     2700

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg     2760

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt     2820

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac     2880

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc     2940

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt     3000

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc     3060

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc     3120

agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg     3180

aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag     3240

atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg     3300

tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt     3360

tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca     3420

tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca     3480

gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc     3540

tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt     3600

ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg     3660

gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc     3720

aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg     3780

ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga     3840

tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga     3900

ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta     3960

aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg     4020

ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact     4080

ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata     4140

agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt     4200

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa     4260

ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt     4320

tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa     4380

tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag     4440

tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg     4500

tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga     4560

ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg     4620

gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg     4680

cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc     4740

cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg     4800

tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa     4860

gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattgt     4920

aatacgactc actatagggc gaattggagc tccaccgcgg tggcggccgc tctagaccag     4980

ccacggcgca tacaaattca cccgtccgcc cacaggcgat gtattacgta tcgaccgcat     5040

caaagaaatc caccaagccc tgcccaatac acacatcgtg atgcacggct ccagctccgt     5100

tccgcaagaa tggctgaaag tcatcaacga atacggcggc aatatcggcg aaacctacgg     5160

cgtgccggtt gaagaaatcg tcgaaggcat caaacacggc gtgcgcaaag tcaacatcga     5220

taccgacttg cgccttgctt ctaccggcgc ggtacgccgc taccttgccg aaaatccgtc     5280

cgactttgac ccgcgcaaat acctgagcaa aaccattgag gccatgaagc aaatctgcct     5340

cgaccgttat cttgcgtttg gctgcgaagg tcaggcaggc aaaatcaaac ctgtttcgtt     5400

ggaaaaaatg gcaagccgtt atgccaaggg cgaattgaac caaatcgtca aataacaggt     5460

tgcctgtaaa caaaatgccg tcccatgggc aaacttaaga gtgtgttgat agtgcagtat     5520

cttaaaattt tgtgtataat aggaattgaa gttaaattag atgctaaaaa tttgtaatta     5580

agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg     5640

ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag attaattcct     5700

accagtgact aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta     5760

acttctgtat ttatttacag atgtaatcac ttcaggagta attacatgaa caaaaatata     5820

aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat aaaacaattg     5880

aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca tttaacgacg     5940

aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca tctattcaac     6000

ttatcgtcag aaaaattaaa actgaacatt cgtgtcactt taattcacca agatattcta     6060

cagtttcaat tccctaacaa acagaggtat aaaattgttg ggagtattcc ttaccattta     6120

agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat ctatctgatt     6180

gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg gttgctcttg     6240

cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt tcatcctaaa     6300

ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt tccagataaa     6360

tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata tcgtcaactg     6420

tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa tttaagtacc     6480

attacttatg agcaagtatt gtctattttt aatagttatc tattatttaa cgggaggaaa     6540

taattctatg agtcgctttt ttaaatttgg aaagttacac gttactaaag ggaatggaga     6600

taaattatta gatatactac tgacagcttc caagaagcta aagaggtccc tttcgacggc     6660

cccgggcctc agc                                                        6673


<210>  44
<211>  5314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-LpxL1KO-MetQ plasmid

<400>  44
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatgaaaacc ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc     1860

tcgcagcctg cggcggtcaa aaagacagcg cgcccgcagc ctctgccgcc gccccttctg     1920

ccgataacgg cgcggcgaaa aaagaaatcg tcttcggcac gaccgtcggc gacttcggcg     1980

atatggtcaa agaacaaatc caagccgagc tggagaaaaa aggctacacc gtcaaattgg     2040

tcgaatttac cgactatgtg cgcccgaatc tggcattggc ggagggcgag ttggacatca     2100

acgtcttcca acacaaaccc tatcttgacg atttcaaaaa agaacacaac ctggacatca     2160

ccgaagcctt ccaagtgccg accgcgcctt tgggactgta tccgggcaaa ctgaaatcgc     2220

tggaagaagt caaagacggc agcaccgtat ccgcgcccaa cgacccgtcc aacttcgcac     2280

gcgccttggt gatgctgaac gaactgggtt ggatcaaact caaagacggc atcaatccgc     2340

tgaccgcatc caaagccgac atcgcggaaa acctgaaaaa catcaaaatc gtcgagcttg     2400

aagccgcaca actgccgcgc agccgcgccg acgtggattt tgccgtcgtc aacggcaact     2460

acgccataag cagcggcatg aagctgaccg aagccctgtt ccaagagccg agctttgcct     2520

atgtcaactg gtctgccgtc aaaaccgccg acaaagacag ccaatggctt aaagacgtaa     2580

ccgaggccta taactccgac gcgttcaaag cctacgcgca caaacgcttc gagggctaca     2640

aataccctgc cgcatggaat gaaggcgcag ccaaataagg cagtcgtata acctgcaggc     2700

ctgcagggaa taatgagtcg acaggatttc ggacgcaacg attcggtttt tgtggatttt     2760

ttcggtattc agacggcaac gattaccgga ttgagccgca ttgccgcgct tgcaaatgca     2820

aaagtgatac ccgccattcc cgtccgcgag gcagacaata cggttacatt gcatttctac     2880

cctgcttgga aatcctttcc gggtgaagac gcgaaagccg acgcgcagcg catgaaccgt     2940

tttatcgaag acagggtgcg cgaacatccg gaacaatatt tttggctgca caagcgtttt     3000

aaaacccgtc cggaaggcag ccccgatttt tactgactac gtcagacggc tctagaggat     3060

ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     3120

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt     3180

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg     3240

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     3300

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     3360

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     3420

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     3480

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     3540

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     3600

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     3660

ctcccttcgg gaagcgtggc gctttctcaa agctcacgct gtaggtatct cagttcggtg     3720

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     3780

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     3840

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     3900

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     3960

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     4020

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     4080

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     4140

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     4200

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     4260

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     4320

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     4380

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     4440

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     4500

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     4560

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     4620

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     4680

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     4740

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     4800

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     4860

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     4920

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     4980

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     5040

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     5100

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     5160

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     5220

acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc     5280

tataaaaata ggcgtatcac gaggcccttt cgtc                                 5314


<210>  45
<211>  5314
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-LpxL1KO-MetQSM plasmid

<400>  45
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatgaaaacc ttcttcaaaa ccctttccgc cgccgcactc gcgctcatcc     1860

tcgcagcctg cggcggtcaa aaagacagcg cgcccgcagc ctctgccgcc gccccttctg     1920

ccgataacgg cgcggcgaaa aaagaaatcg tcttcggcac gaccgtcggc gacttcggcg     1980

atatggtcaa agaacaaatc caagccgagc tggagaaaaa aggctacacc gtcaaattgg     2040

tcgaatttac cgactatgtg cgcccgaatc tggcattggc ggagggcgag ttggacatca     2100

acgtcttcca acacaaaccc tatcttgacg atttcaaaaa agaacacaac ctggacatca     2160

ccgaagcctt ccaagtgccg accgcgcctt tgggactgta tccgggcaaa ctgaaatcgc     2220

tggaagaagt caaagacggc agcaccgtat ccgcgcccaa cgacccgtcc aacttcgcac     2280

gcgccttggt gatgctgaac gaactgggtt ggatcaaact caaagacggc atcaatccgc     2340

tgaccgcatc caaagccgac atcgcggaaa acctgaaaaa catcaaaatc gtcgagcttg     2400

aagccgcaca actgccgcgc agccgcgccg acgtggattt tgccgtcgtc aacggcaact     2460

acgccataag cagcggcatg aagctgaccg aagccctgtt ccaagagccg agctttgcct     2520

atgtcgcctg gtctgccgtc aaaaccgccg acaaagacag ccaatggctt aaagacgtaa     2580

ccgaggccta taactccgac gcgttcaaag cctacgcgca caaacgcttc gagggctaca     2640

aataccctgc cgcatggaat gaaggcgcag ccaaataagg cagtcgtata acctgcaggc     2700

ctgcagggaa taatgagtcg acaggatttc ggacgcaacg attcggtttt tgtggatttt     2760

ttcggtattc agacggcaac gattaccgga ttgagccgca ttgccgcgct tgcaaatgca     2820

aaagtgatac ccgccattcc cgtccgcgag gcagacaata cggttacatt gcatttctac     2880

cctgcttgga aatcctttcc gggtgaagac gcgaaagccg acgcgcagcg catgaaccgt     2940

tttatcgaag acagggtgcg cgaacatccg gaacaatatt tttggctgca caagcgtttt     3000

aaaacccgtc cggaaggcag ccccgatttt tactgactac gtcagacggc tctagaggat     3060

ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt     3120

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt     3180

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg     3240

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg     3300

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg     3360

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat     3420

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc     3480

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc     3540

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     3600

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     3660

ctcccttcgg gaagcgtggc gctttctcaa agctcacgct gtaggtatct cagttcggtg     3720

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     3780

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     3840

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     3900

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     3960

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     4020

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     4080

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt     4140

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa     4200

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa     4260

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc     4320

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct     4380

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca     4440

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt     4500

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt     4560

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc     4620

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc     4680

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt     4740

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact     4800

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc     4860

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt     4920

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg     4980

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct     5040

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa     5100

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt     5160

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc     5220

acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc     5280

tataaaaata ggcgtatcac gaggcccttt cgtc                                 5314


<210>  46
<211>  5395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-LpxL1KO-GNA1220 plasmid

<400>  46
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatggaattt ttcattatct tgttggcagc cgtcgccgtt ttcggcttca     1860

aatcctttgt cgtcatcccc cagcaggaag tccacgttgt cgaaaggctc gggcgtttcc     1920

atcgcgccct gacggccggt ttgaatattt tgattccctt tatcgaccgc gtcgcctacc     1980

gccattcgct gaaagaaatc cctttagacg tacccagcca ggtctgcatc acgcgcgata     2040

atacgcaatt gactgttgac ggcatcatct atttccaagt aaccgatccc aaactcgcct     2100

catacggttc gagcaactac attatggcaa ttacccagct tgcccaaacg acgctgcgtt     2160

ccgttatcgg gcgtatggag ttggacaaaa cgtttgaaga acgcgacgaa atcaacagta     2220

ccgtcgtctc cgccctcgat gaagccgccg gggcttgggg tgtgaaagtc ctccgttacg     2280

aaatcaagga tttggttccg ccgcaagaaa tccttcgcgc aatgcaggca caaattaccg     2340

ccgaacgcga aaaacgcgcc cgtattgccg aatccgaagg ccgtaaaatc gaacaaatca     2400

accttgccag tggtcagcgt gaagccgaaa tccaacaatc cgaaggcgag gctcaggctg     2460

cggtcaatgc gtccaatgcc gagaaaatcg cccgcatcaa ccgcgccaaa ggcgaagcgg     2520

aatccctgcg ccttgttgcc gaagccaatg ccgaagccat ccgtcaaatt gccgccgccc     2580

ttcaaaccca aggcggggcg gatgcggtca atctgaagat tgcggaacaa tacgtagccg     2640

cgttcaacaa tcttgccaaa gaaagcaata cgctgattat gcccgccaat gttgccgaca     2700

tcggcagcct gatttctgcc ggcatgaaaa ttatcgacag cagcaaaacc gccaaataag     2760

gcagtcgtat aacctgcagg cctgcaggga ataatgagtc gacaggattt cggacgcaac     2820

gattcggttt ttgtggattt tttcggtatt cagacggcaa cgattaccgg attgagccgc     2880

attgccgcgc ttgcaaatgc aaaagtgata cccgccattc ccgtccgcga ggcagacaat     2940

acggttacat tgcatttcta ccctgcttgg aaatcctttc cgggtgaaga cgcgaaagcc     3000

gacgcgcagc gcatgaaccg ttttatcgaa gacagggtgc gcgaacatcc ggaacaatat     3060

ttttggctgc acaagcgttt taaaacccgt ccggaaggca gccccgattt ttactgacta     3120

cgtcagacgg ctctagagga tccccgggta ccgagctcga attcgtaatc atggtcatag     3180

ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc     3240

ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc     3300

tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa     3360

cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg     3420

ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg     3480

ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag     3540

gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac     3600

gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga     3660

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     3720

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca aagctcacgc     3780

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     3840

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     3900

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     3960

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     4020

gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct     4080

tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt     4140

acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct     4200

cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc     4260

acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa     4320

acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta     4380

tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc     4440

ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat     4500

ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta     4560

tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt     4620

aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt     4680

ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg     4740

ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc     4800

gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc     4860

gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg     4920

cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga     4980

actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta     5040

ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct     5100

tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag     5160

ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga     5220

agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat     5280

aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc     5340

attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc          5395


<210>  47
<211>  5721
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pUC18-LpxL1KO-NHba plasmid

<400>  47
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc      240

attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat      300

tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt      360

tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatgtgt atcgagatga      420

aatttatatt ttttgtactg tatgttttgc agtttctgcc gtttgcgctg ctgcacaaga      480

ttgccgacct gacgggtttg cttgcctacc ttctggtcaa accgcgccgc cgtatcggcg      540

aaatcaattt ggcaaaatgt ttttccgaat ggagtgagga aaagcgtaaa accgtgttga      600

aacagcattt caaacacatg gcgaaactga tgttggaata cggtttatat tggtacgcgc      660

ctgccggacg tttgaaatcg ctggtgcgct accgcaataa gcattatttg gacgacgcgc      720

tggcggcggg ggaaaaagtc atcatcctgt atccgcactt caccgctgca gttctcgagg      780

cagtgactaa ctaggaggaa taaatggcta aaatgagaat atcaccggaa ttgaaaaaac      840

tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct aaggtatata      900

agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg tataaaggga      960

ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga aagctgcctg     1020

ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc atgagtgagg     1080

ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa aagattatcg     1140

agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat tgtccctata     1200

cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac gatctggccg     1260

atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc gagctgtatg     1320

attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc gacctgggag     1380

acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt gggagaagcg     1440

gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg gaggatatcg     1500

gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct gattgggaga     1560

aaataaaata ttatatttta ctggatgaat tgttttagta cccctcagcg catgcatccg     1620

ggctctgcgc ttgaattgtg ttgtagaaac acaacgtttt tgaaaaaata agctattgtt     1680

ttatatcaaa atataatcat ttttaaaata aaggttgcgg catttatcag atatttgttc     1740

tgaaatgaag acgtatcggg tgtttgcccg atgtttttag gtttttatca aatttacaaa     1800

aggaagccca tatgtttaaa cgcagtgtga ttgcaatggc ttgtattttt cccctttcag     1860

cctgtggggg cggcggtggc ggatcgcccg atgtcaagtc ggcggacacg ccgtcaaaac     1920

cggccgcccc cgttgttgct gaaaatgccg gggaaggggt gctgccgaaa gaaaagaaag     1980

atgaggaggc agcgggcggt gcgccgcaag ccgatacgca ggacgcaacc gccggagaag     2040

gcagccaaga tatggcggca gtttcggcag aaaatacagg caatggcggt gcggcaacaa     2100

cggacaaccc caaaaatgaa gacgcggggg cgcaaaatga tatgccgcaa aatgccgccg     2160

aatccgcaaa tcaaacaggg aacaaccaac ccgccggttc ttcagattcc gcccccgcgt     2220

caaaccctgc ccctgcgaat ggcggtagcg attttggaag gacgaacgtg ggcaattctg     2280

ttgtgattga cggaccgtcg caaaatataa cgttgaccca ctgtaaaggc gatccttgta     2340

atggtgataa tttattggat gaagaagcac cgccaaaatc agaatttgaa tcgttaagtg     2400

atgaagagaa aattaagaaa tataaaaaag atggagaaaa gtttactggt ttggttgcta     2460

taaaagttga aaataatggg ttgaataaat ataccattat ttatcaagct cagcctactc     2520

gttctgcacg gtcgaggagg tcgcttccgg ccgagattcc gctgattccc gtcaatcagg     2580

ccgatacgct gattgtggat ggggaagcgg tcagcctgac ggggcattcc ggcaatatct     2640

tcgcgcccga agggaattac cggtatctga cttacggggc ggaaaaattg cccggcggat     2700

cgtatgccct ccgtgtgcaa ggcgaaccgg caaaaggcga aatgcttgcg ggcacggccg     2760

tgtacaacgg cgaagtgctg catttccata tggaaaacgg ccgtccgtac ccgtccggag     2820

gcaggtttgc cgcaaaagtc gatttcggca gcaaatctgt ggacggcatt atcgacagca     2880

gcgatgattt gcatatgggt acgcaaaaat tcaaagccgc catcgatgga aacggcttta     2940

aggggacttg gacggaaaat ggcggcgggg atgtttccgg aaggttttac ggcccggccg     3000

gcgaggaagt ggcgggaaaa tacagctatc gcccgacaga tgctgaaaag ggcggattcg     3060

gcgtgtttgc cggcaaaaaa gatcgggatt gaaggcctcc tgcaggcctg cagggaataa     3120

tgagtcgaca ggatttcgga cgcaacgatt cggtttttgt ggattttttc ggtattcaga     3180

cggcaacgat taccggattg agccgcattg ccgcgcttgc aaatgcaaaa gtgatacccg     3240

ccattcccgt ccgcgaggca gacaatacgg ttacattgca tttctaccct gcttggaaat     3300

cctttccggg tgaagacgcg aaagccgacg cgcagcgcat gaaccgtttt atcgaagaca     3360

gggtgcgcga acatccggaa caatattttt ggctgcacaa gcgttttaaa acccgtccgg     3420

aaggcagccc cgatttttac tgactacgtc agacggctct agaggatccc cgggtaccga     3480

gctcgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa     3540

ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga     3600

gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt     3660

gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct     3720

cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat     3780

cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga     3840

acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt     3900

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt     3960

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     4020

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     4080

gcgtggcgct ttctcaaagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     4140

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     4200

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     4260

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     4320

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     4380

ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg     4440

gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt     4500

tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg     4560

tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta     4620

aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg     4680

aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg     4740

tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc     4800

gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg     4860

agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg     4920

aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag     4980

gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat     5040

caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc     5100

cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc     5160

ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa     5220

ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac     5280

gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt     5340

cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc     5400

gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa     5460

caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca     5520

tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat     5580

acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa     5640

aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc     5700

gtatcacgag gccctttcgt c                                               5721


<210>  48
<211>  6531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GalEKO-MetQ plasmid

<400>  48
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg aaaaccttct tcaaaaccct ttccgccgcc gcactcgcgc tcatcctcgc      720

agcctgcggc ggtcaaaaag acagcgcgcc cgcagcctct gccgccgccc cttctgccga      780

taacggcgcg gcgaaaaaag aaatcgtctt cggcacgacc gtcggcgact tcggcgatat      840

ggtcaaagaa caaatccaag ccgagctgga gaaaaaaggc tacaccgtca aattggtcga      900

atttaccgac tatgtgcgcc cgaatctggc attggcggag ggcgagttgg acatcaacgt      960

cttccaacac aaaccctatc ttgacgattt caaaaaagaa cacaacctgg acatcaccga     1020

agccttccaa gtgccgaccg cgcctttggg actgtatccg ggcaaactga aatcgctgga     1080

agaagtcaaa gacggcagca ccgtatccgc gcccaacgac ccgtccaact tcgcacgcgc     1140

cttggtgatg ctgaacgaac tgggttggat caaactcaaa gacggcatca atccgctgac     1200

cgcatccaaa gccgacatcg cggaaaacct gaaaaacatc aaaatcgtcg agcttgaagc     1260

cgcacaactg ccgcgcagcc gcgccgacgt ggattttgcc gtcgtcaacg gcaactacgc     1320

cataagcagc ggcatgaagc tgaccgaagc cctgttccaa gagccgagct ttgcctatgt     1380

caactggtct gccgtcaaaa ccgccgacaa agacagccaa tggcttaaag acgtaaccga     1440

ggcctataac tccgacgcgt tcaaagccta cgcgcacaaa cgcttcgagg gctacaaata     1500

ccctgccgca tggaatgaag gcgcagccaa ataaggcagt cgtataatct agatctagag     1560

acgatgccgt ctgaagaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact     1620

tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc     1680

cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc     1740

aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg     1800

gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg     1860

cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc     1920

agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact     1980

caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta     2040

catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg     2100

gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg     2160

gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt     2220

gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa     2280

tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg     2340

gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag     2400

gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg     2460

ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc     2520

cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg     2580

gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat     2640

cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac     2700

tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctatgccg tctgaatact     2760

cgagcggaac ccgggtagtg gcctgtaaaa gacagcatat agagatgagc aggctgtata     2820

atattaagga tttttttgta acttctataa atataaagta attttttagg agttatatta     2880

ttagggcttc taggaagctc aaatagataa atagattcaa atagattctt gttagctgat     2940

tgatgaacta acttaggcat ttttaagttt ttagaagtat ataaaattac tagtaaatta     3000

ttggttaatt tttgtatttt aattaggctt tggacttggt taagctgacc taaattagat     3060

atgacaaata aattgttacg tgggggggta agataaaatg gagatgttgt caaccacatt     3120

gaatcttgaa aaaacttttt aggctgaaaa agagcttttt ttattttctt tagcattatt     3180

gtatctctta aaaattaatg agaattagct atatgtaata gccaatcctc tgttaataaa     3240

gtaactaagt taataagcat tattcaatat cagttttttt gatttgagca cctttgcgaa     3300

tattgcaagc agcgacctta ccaaataatg tttcatattc gttgacgctg aagtctccat     3360

tgcctgggcg tttaacccat aggttatcta agcttgagta ttctatagtg tcacctaaat     3420

agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt     3480

ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc     3540

taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc     3600

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct     3660

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     3720

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     3780

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     3840

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     3900

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     3960

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     4020

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     4080

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     4140

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     4200

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     4260

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc     4320

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     4380

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     4440

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     4500

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     4560

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     4620

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     4680

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     4740

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     4800

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     4860

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     4920

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     4980

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     5040

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     5100

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     5160

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     5220

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     5280

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     5340

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     5400

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     5460

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     5520

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     5580

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt     5640

atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg     5700

cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt     5760

cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag     5820

cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga     5880

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt     5940

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa     6000

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa     6060

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac     6120

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga     6180

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa     6240

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc     6300

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc     6360

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca     6420

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca     6480

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat a              6531


<210>  49
<211>  6531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GlaEKO-MetQSM plasmid

<400>  49
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg aaaaccttct tcaaaaccct ttccgccgcc gcactcgcgc tcatcctcgc      720

agcctgcggc ggtcaaaaag acagcgcgcc cgcagcctct gccgccgccc cttctgccga      780

taacggcgcg gcgaaaaaag aaatcgtctt cggcacgacc gtcggcgact tcggcgatat      840

ggtcaaagaa caaatccaag ccgagctgga gaaaaaaggc tacaccgtca aattggtcga      900

atttaccgac tatgtgcgcc cgaatctggc attggcggag ggcgagttgg acatcaacgt      960

cttccaacac aaaccctatc ttgacgattt caaaaaagaa cacaacctgg acatcaccga     1020

agccttccaa gtgccgaccg cgcctttggg actgtatccg ggcaaactga aatcgctgga     1080

agaagtcaaa gacggcagca ccgtatccgc gcccaacgac ccgtccaact tcgcacgcgc     1140

cttggtgatg ctgaacgaac tgggttggat caaactcaaa gacggcatca atccgctgac     1200

cgcatccaaa gccgacatcg cggaaaacct gaaaaacatc aaaatcgtcg agcttgaagc     1260

cgcacaactg ccgcgcagcc gcgccgacgt ggattttgcc gtcgtcaacg gcaactacgc     1320

cataagcagc ggcatgaagc tgaccgaagc cctgttccaa gagccgagct ttgcctatgt     1380

cgcctggtct gccgtcaaaa ccgccgacaa agacagccaa tggcttaaag acgtaaccga     1440

ggcctataac tccgacgcgt tcaaagccta cgcgcacaaa cgcttcgagg gctacaaata     1500

ccctgccgca tggaatgaag gcgcagccaa ataaggcagt cgtataatct agatctagag     1560

acgatgccgt ctgaagaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact     1620

tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc     1680

cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc     1740

aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg     1800

gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg     1860

cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc     1920

agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact     1980

caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta     2040

catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg     2100

gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg     2160

gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt     2220

gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa     2280

tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg     2340

gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag     2400

gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg     2460

ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc     2520

cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg     2580

gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat     2640

cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac     2700

tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctatgccg tctgaatact     2760

cgagcggaac ccgggtagtg gcctgtaaaa gacagcatat agagatgagc aggctgtata     2820

atattaagga tttttttgta acttctataa atataaagta attttttagg agttatatta     2880

ttagggcttc taggaagctc aaatagataa atagattcaa atagattctt gttagctgat     2940

tgatgaacta acttaggcat ttttaagttt ttagaagtat ataaaattac tagtaaatta     3000

ttggttaatt tttgtatttt aattaggctt tggacttggt taagctgacc taaattagat     3060

atgacaaata aattgttacg tgggggggta agataaaatg gagatgttgt caaccacatt     3120

gaatcttgaa aaaacttttt aggctgaaaa agagcttttt ttattttctt tagcattatt     3180

gtatctctta aaaattaatg agaattagct atatgtaata gccaatcctc tgttaataaa     3240

gtaactaagt taataagcat tattcaatat cagttttttt gatttgagca cctttgcgaa     3300

tattgcaagc agcgacctta ccaaataatg tttcatattc gttgacgctg aagtctccat     3360

tgcctgggcg tttaacccat aggttatcta agcttgagta ttctatagtg tcacctaaat     3420

agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt     3480

ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc     3540

taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc     3600

cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct     3660

tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca     3720

gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac     3780

atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt     3840

ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg     3900

cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc     3960

tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc     4020

gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc     4080

aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac     4140

tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt     4200

aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct     4260

aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc     4320

ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt     4380

ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg     4440

atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc     4500

atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa     4560

tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag     4620

gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg     4680

tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga     4740

gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag     4800

cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa     4860

gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc     4920

atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca     4980

aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg     5040

atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat     5100

aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc     5160

aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg     5220

gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg     5280

gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt     5340

gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca     5400

ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata     5460

ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac     5520

atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa     5580

gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt     5640

atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg     5700

cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt     5760

cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag     5820

cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga     5880

aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt     5940

gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa     6000

aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa     6060

agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac     6120

gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga     6180

accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa     6240

aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc     6300

tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc     6360

attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca     6420

gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca     6480

gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat a              6531


<210>  50
<211>  6612
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GlaEKO-GNA1220 plasmid

<400>  50
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg gaatttttca ttatcttgtt ggcagccgtc gccgttttcg gcttcaaatc      720

ctttgtcgtc atcccccagc aggaagtcca cgttgtcgaa aggctcgggc gtttccatcg      780

cgccctgacg gccggtttga atattttgat tccctttatc gaccgcgtcg cctaccgcca      840

ttcgctgaaa gaaatccctt tagacgtacc cagccaggtc tgcatcacgc gcgataatac      900

gcaattgact gttgacggca tcatctattt ccaagtaacc gatcccaaac tcgcctcata      960

cggttcgagc aactacatta tggcaattac ccagcttgcc caaacgacgc tgcgttccgt     1020

tatcgggcgt atggagttgg acaaaacgtt tgaagaacgc gacgaaatca acagtaccgt     1080

cgtctccgcc ctcgatgaag ccgccggggc ttggggtgtg aaagtcctcc gttacgaaat     1140

caaggatttg gttccgccgc aagaaatcct tcgcgcaatg caggcacaaa ttaccgccga     1200

acgcgaaaaa cgcgcccgta ttgccgaatc cgaaggccgt aaaatcgaac aaatcaacct     1260

tgccagtggt cagcgtgaag ccgaaatcca acaatccgaa ggcgaggctc aggctgcggt     1320

caatgcgtcc aatgccgaga aaatcgcccg catcaaccgc gccaaaggcg aagcggaatc     1380

cctgcgcctt gttgccgaag ccaatgccga agccatccgt caaattgccg ccgcccttca     1440

aacccaaggc ggggcggatg cggtcaatct gaagattgcg gaacaatacg tagccgcgtt     1500

caacaatctt gccaaagaaa gcaatacgct gattatgccc gccaatgttg ccgacatcgg     1560

cagcctgatt tctgccggca tgaaaattat cgacagcagc aaaaccgcca aataaggcag     1620

tcgtataatc tagatctaga gacgatgccg tctgaagaaa ccttgcgctc gttcgccagc     1680

caggacagaa atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg     1740

aaacggatga aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc     1800

aagtagcgta tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac     1860

ggcgcagtgg cggttttcat ggcttgttat gactgttttt ttggggtaca gtctatgcct     1920

cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc     1980

aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gagggaagcg     2040

gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc     2100

gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca     2160

cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga     2220

gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc     2280

gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag     2340

cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca     2400

gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc     2460

ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag     2520

gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga     2580

aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg     2640

aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata     2700

cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat     2760

cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa     2820

tgtctatgcc gtctgaatac tcgagcggaa cccgggtagt ggcctgtaaa agacagcata     2880

tagagatgag caggctgtat aatattaagg atttttttgt aacttctata aatataaagt     2940

aattttttag gagttatatt attagggctt ctaggaagct caaatagata aatagattca     3000

aatagattct tgttagctga ttgatgaact aacttaggca tttttaagtt tttagaagta     3060

tataaaatta ctagtaaatt attggttaat ttttgtattt taattaggct ttggacttgg     3120

ttaagctgac ctaaattaga tatgacaaat aaattgttac gtgggggggt aagataaaat     3180

ggagatgttg tcaaccacat tgaatcttga aaaaactttt taggctgaaa aagagctttt     3240

tttattttct ttagcattat tgtatctctt aaaaattaat gagaattagc tatatgtaat     3300

agccaatcct ctgttaataa agtaactaag ttaataagca ttattcaata tcagtttttt     3360

tgatttgagc acctttgcga atattgcaag cagcgacctt accaaataat gtttcatatt     3420

cgttgacgct gaagtctcca ttgcctgggc gtttaaccca taggttatct aagcttgagt     3480

attctatagt gtcacctaaa tagcttggcg taatcatggt catagctgtt tcctgtgtga     3540

aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc     3600

tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc     3660

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc     3720

ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt     3780

cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca     3840

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa     3900

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     3960

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     4020

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     4080

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     4140

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     4200

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     4260

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     4320

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     4380

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa     4440

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa     4500

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac     4560

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta     4620

aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt     4680

taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata     4740

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc     4800

agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac     4860

cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag     4920

tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac     4980

gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc     5040

agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg     5100

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc     5160

atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct     5220

gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc     5280

tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc     5340

atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc     5400

agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc     5460

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca     5520

cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt     5580

tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt     5640

ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca     5700

ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac     5760

ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat     5820

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg     5880

cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata     5940

ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt     6000

taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg     6060

gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt     6120

ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct     6180

atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt     6240

gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa     6300

agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc     6360

tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc     6420

tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg     6480

ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg     6540

ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata     6600

cgactcacta ta                                                         6612


<210>  51
<211>  6938
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGEM-SiaD-GlaEKO-NHba plasmid

<400>  51
gggcgaattc ctggacacgg cagaccgata aagcccgcac cgccggtaac gaggatggtt       60

tttttgtttg cggtttgcat ttaatcccca tatccgttgg ggttgcggct gacccaacgc      120

cacgaatctt ccatcatttg ctgcaagccg cgtttggttt cccagccggt ttgttgtttg      180

gtatgggacg ggtcggcaaa ggaacacgcc aaatcaccgg cgcggcgcgg tttgatttca      240

aatggaatgg ttaaacccga agccgcttca aatgcgcgga tgatttccaa taccgaagaa      300

gcgcggccgg agcctaagtt cagcaaatgc gtgcctgcta cattactttt tgcctgcata      360

gccgcgacat ggccttctgc caaatccatc acatgaatat agtcacgcat ccccgtgccg      420

tcgggggtag ggtagtcatc gccaaatacc gccaattgcc tcagcgcatg catccgggct      480

ctgcgcttga attgtgttgt agaaacacaa cgtttttgaa aaaataagct attgttttat      540

atcaaaatat aatcattttt aaaataaagg ttgcggcatt tatcagatat ttgttctgaa      600

atgaagacgt atcgggtgtt tgcccgatgt ttttaggttt ttatcaaatt tacaaaagga      660

agcccatatg tttaaacgca gtgtgattgc aatggcttgt atttttcccc tttcagcctg      720

tgggggcggc ggtggcggat cgcccgatgt caagtcggcg gacacgccgt caaaaccggc      780

cgcccccgtt gttgctgaaa atgccgggga aggggtgctg ccgaaagaaa agaaagatga      840

ggaggcagcg ggcggtgcgc cgcaagccga tacgcaggac gcaaccgccg gagaaggcag      900

ccaagatatg gcggcagttt cggcagaaaa tacaggcaat ggcggtgcgg caacaacgga      960

caaccccaaa aatgaagacg cgggggcgca aaatgatatg ccgcaaaatg ccgccgaatc     1020

cgcaaatcaa acagggaaca accaacccgc cggttcttca gattccgccc ccgcgtcaaa     1080

ccctgcccct gcgaatggcg gtagcgattt tggaaggacg aacgtgggca attctgttgt     1140

gattgacgga ccgtcgcaaa atataacgtt gacccactgt aaaggcgatc cttgtaatgg     1200

tgataattta ttggatgaag aagcaccgcc aaaatcagaa tttgaatcgt taagtgatga     1260

agagaaaatt aagaaatata aaaaagatgg agaaaagttt actggtttgg ttgctataaa     1320

agttgaaaat aatgggttga ataaatatac cattatttat caagctcagc ctactcgttc     1380

tgcacggtcg aggaggtcgc ttccggccga gattccgctg attcccgtca atcaggccga     1440

tacgctgatt gtggatgggg aagcggtcag cctgacgggg cattccggca atatcttcgc     1500

gcccgaaggg aattaccggt atctgactta cggggcggaa aaattgcccg gcggatcgta     1560

tgccctccgt gtgcaaggcg aaccggcaaa aggcgaaatg cttgcgggca cggccgtgta     1620

caacggcgaa gtgctgcatt tccatatgga aaacggccgt ccgtacccgt ccggaggcag     1680

gtttgccgca aaagtcgatt tcggcagcaa atctgtggac ggcattatcg acagcagcga     1740

tgatttgcat atgggtacgc aaaaattcaa agccgccatc gatggaaacg gctttaaggg     1800

gacttggacg gaaaatggcg gcggggatgt ttccggaagg ttttacggcc cggccggcga     1860

ggaagtggcg ggaaaataca gctatcgccc gacagatgct gaaaagggcg gattcggcgt     1920

gtttgccggc aaaaaagatc gggattgaag gccttctaga tctagagacg atgccgtctg     1980

aagaaacctt gcgctcgttc gccagccagg acagaaatgc ctcgacttcg ctgctgccca     2040

aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag     2100

cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa     2160

ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact     2220

gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg     2280

gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa     2340

caaagttaaa catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg     2400

tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct     2460

ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg     2520

taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt     2580

cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca     2640

tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg     2700

acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga     2760

caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc     2820

cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc     2880

cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca     2940

gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc     3000

tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag     3060

aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg     3120

agatcaccaa ggtagtcggc aaataatgtc tatgccgtct gaatactcga gcggaacccg     3180

ggtagtggcc tgtaaaagac agcatataga gatgagcagg ctgtataata ttaaggattt     3240

ttttgtaact tctataaata taaagtaatt ttttaggagt tatattatta gggcttctag     3300

gaagctcaaa tagataaata gattcaaata gattcttgtt agctgattga tgaactaact     3360

taggcatttt taagttttta gaagtatata aaattactag taaattattg gttaattttt     3420

gtattttaat taggctttgg acttggttaa gctgacctaa attagatatg acaaataaat     3480

tgttacgtgg gggggtaaga taaaatggag atgttgtcaa ccacattgaa tcttgaaaaa     3540

actttttagg ctgaaaaaga gcttttttta ttttctttag cattattgta tctcttaaaa     3600

attaatgaga attagctata tgtaatagcc aatcctctgt taataaagta actaagttaa     3660

taagcattat tcaatatcag tttttttgat ttgagcacct ttgcgaatat tgcaagcagc     3720

gaccttacca aataatgttt catattcgtt gacgctgaag tctccattgc ctgggcgttt     3780

aacccatagg ttatctaagc ttgagtattc tatagtgtca cctaaatagc ttggcgtaat     3840

catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac     3900

gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa     3960

ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat     4020

gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc     4080

tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg     4140

cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag     4200

gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc     4260

gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag     4320

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     4380

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     4440

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     4500

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     4560

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     4620

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     4680

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag     4740

ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca     4800

agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg     4860

ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa     4920

aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta     4980

tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag     5040

cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga     5100

tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac     5160

cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc     5220

ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta     5280

gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac     5340

gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat     5400

gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa     5460

gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg     5520

tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag     5580

aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc     5640

cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct     5700

caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat     5760

cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg     5820

ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc     5880

aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta     5940

tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg     6000

tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct     6060

ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga     6120

cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag     6180

cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga     6240

gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca     6300

ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     6360

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga     6420

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc     6480

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc     6540

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag     6600

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa     6660

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac     6720

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc     6780

aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg     6840

ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt     6900

aaaacgacgg ccagtgaatt gtaatacgac tcactata                             6938


<210>  52
<211>  7659
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-MetQ plasmid

<400>  52
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atgaaaacct     4380

tcttcaaaac cctttccgcc gccgcactcg cgctcatcct cgcagcctgc ggcggtcaaa     4440

aagacagcgc gcccgcagcc tctgccgccg ccccttctgc cgataacggc gcggcgaaaa     4500

aagaaatcgt cttcggcacg accgtcggcg acttcggcga tatggtcaaa gaacaaatcc     4560

aagccgagct ggagaaaaaa ggctacaccg tcaaattggt cgaatttacc gactatgtgc     4620

gcccgaatct ggcattggcg gagggcgagt tggacatcaa cgtcttccaa cacaaaccct     4680

atcttgacga tttcaaaaaa gaacacaacc tggacatcac cgaagccttc caagtgccga     4740

ccgcgccttt gggactgtat ccgggcaaac tgaaatcgct ggaagaagtc aaagacggca     4800

gcaccgtatc cgcgcccaac gacccgtcca acttcgcacg cgccttggtg atgctgaacg     4860

aactgggttg gatcaaactc aaagacggca tcaatccgct gaccgcatcc aaagccgaca     4920

tcgcggaaaa cctgaaaaac atcaaaatcg tcgagcttga agccgcacaa ctgccgcgca     4980

gccgcgccga cgtggatttt gccgtcgtca acggcaacta cgccataagc agcggcatga     5040

agctgaccga agccctgttc caagagccga gctttgccta tgtcaactgg tctgccgtca     5100

aaaccgccga caaagacagc caatggctta aagacgtaac cgaggcctat aactccgacg     5160

cgttcaaagc ctacgcgcac aaacgcttcg agggctacaa ataccctgcc gcatggaatg     5220

aaggcgcagc caaataaggc agtcgtataa cctgcaggac tagtcggccg tacctgtcag     5280

accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga     5340

tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt     5400

tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc     5460

tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc     5520

cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac     5580

caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac     5640

cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt     5700

cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct     5760

gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat     5820

acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt     5880

atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg     5940

cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt     6000

gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt     6060

tcctggcctt ttgctggcct tttgctcaca tgtgggccct ttcgtctcgc gcgtttcggt     6120

gatagcttaa gtgcggtcat cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag     6180

tcccctacgt gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg     6240

atactatgac tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag     6300

tccgcaccgc tgccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac     6360

ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc     6420

ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt     6480

tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa     6540

cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat     6600

tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt     6660

cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta     6720

acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc     6780

cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct     6840

taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt     6900

ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc     6960

accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc     7020

atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa     7080

aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca     7140

tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat     7200

gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc     7260

accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca     7320

agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag     7380

gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc     7440

tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt     7500

ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt     7560

agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat     7620

tttcgccaaa agttggccca gggcttcccg gtatcaaca                            7659


<210>  53
<211>  7659
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-MetQSM plasmid

<400>  53
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atgaaaacct     4380

tcttcaaaac cctttccgcc gccgcactcg cgctcatcct cgcagcctgc ggcggtcaaa     4440

aagacagcgc gcccgcagcc tctgccgccg ccccttctgc cgataacggc gcggcgaaaa     4500

aagaaatcgt cttcggcacg accgtcggcg acttcggcga tatggtcaaa gaacaaatcc     4560

aagccgagct ggagaaaaaa ggctacaccg tcaaattggt cgaatttacc gactatgtgc     4620

gcccgaatct ggcattggcg gagggcgagt tggacatcaa cgtcttccaa cacaaaccct     4680

atcttgacga tttcaaaaaa gaacacaacc tggacatcac cgaagccttc caagtgccga     4740

ccgcgccttt gggactgtat ccgggcaaac tgaaatcgct ggaagaagtc aaagacggca     4800

gcaccgtatc cgcgcccaac gacccgtcca acttcgcacg cgccttggtg atgctgaacg     4860

aactgggttg gatcaaactc aaagacggca tcaatccgct gaccgcatcc aaagccgaca     4920

tcgcggaaaa cctgaaaaac atcaaaatcg tcgagcttga agccgcacaa ctgccgcgca     4980

gccgcgccga cgtggatttt gccgtcgtca acggcaacta cgccataagc agcggcatga     5040

agctgaccga agccctgttc caagagccga gctttgccta tgtcgcctgg tctgccgtca     5100

aaaccgccga caaagacagc caatggctta aagacgtaac cgaggcctat aactccgacg     5160

cgttcaaagc ctacgcgcac aaacgcttcg agggctacaa ataccctgcc gcatggaatg     5220

aaggcgcagc caaataaggc agtcgtataa cctgcaggac tagtcggccg tacctgtcag     5280

accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga     5340

tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt     5400

tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc     5460

tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc     5520

cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac     5580

caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac     5640

cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt     5700

cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct     5760

gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat     5820

acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt     5880

atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg     5940

cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt     6000

gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt     6060

tcctggcctt ttgctggcct tttgctcaca tgtgggccct ttcgtctcgc gcgtttcggt     6120

gatagcttaa gtgcggtcat cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag     6180

tcccctacgt gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg     6240

atactatgac tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag     6300

tccgcaccgc tgccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac     6360

ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc     6420

ccccggccac ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt     6480

tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa     6540

cgaagcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat     6600

tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt     6660

cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta     6720

acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc     6780

cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct     6840

taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt     6900

ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc     6960

accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc     7020

atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa     7080

aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca     7140

tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat     7200

gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc     7260

accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca     7320

agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag     7380

gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc     7440

tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt     7500

ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt     7560

agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat     7620

tttcgccaaa agttggccca gggcttcccg gtatcaaca                            7659


<210>  54
<211>  7740
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-GNA1220 plasmid

<400>  54
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atggaatttt     4380

tcattatctt gttggcagcc gtcgccgttt tcggcttcaa atcctttgtc gtcatccccc     4440

agcaggaagt ccacgttgtc gaaaggctcg ggcgtttcca tcgcgccctg acggccggtt     4500

tgaatatttt gattcccttt atcgaccgcg tcgcctaccg ccattcgctg aaagaaatcc     4560

ctttagacgt acccagccag gtctgcatca cgcgcgataa tacgcaattg actgttgacg     4620

gcatcatcta tttccaagta accgatccca aactcgcctc atacggttcg agcaactaca     4680

ttatggcaat tacccagctt gcccaaacga cgctgcgttc cgttatcggg cgtatggagt     4740

tggacaaaac gtttgaagaa cgcgacgaaa tcaacagtac cgtcgtctcc gccctcgatg     4800

aagccgccgg ggcttggggt gtgaaagtcc tccgttacga aatcaaggat ttggttccgc     4860

cgcaagaaat ccttcgcgca atgcaggcac aaattaccgc cgaacgcgaa aaacgcgccc     4920

gtattgccga atccgaaggc cgtaaaatcg aacaaatcaa ccttgccagt ggtcagcgtg     4980

aagccgaaat ccaacaatcc gaaggcgagg ctcaggctgc ggtcaatgcg tccaatgccg     5040

agaaaatcgc ccgcatcaac cgcgccaaag gcgaagcgga atccctgcgc cttgttgccg     5100

aagccaatgc cgaagccatc cgtcaaattg ccgccgccct tcaaacccaa ggcggggcgg     5160

atgcggtcaa tctgaagatt gcggaacaat acgtagccgc gttcaacaat cttgccaaag     5220

aaagcaatac gctgattatg cccgccaatg ttgccgacat cggcagcctg atttctgccg     5280

gcatgaaaat tatcgacagc agcaaaaccg ccaaataagg cagtcgtata acctgcagga     5340

ctagtcggcc gtacctgtca gaccaagttt actcatatat actttagatt gatttaaaac     5400

ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa     5460

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat     5520

cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc     5580

taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg     5640

gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc     5700

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg     5760

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg     5820

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa     5880

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg     5940

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga     6000

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct     6060

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca     6120

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtgggccc     6180

tttcgtctcg cgcgtttcgg tgatagctta agtgcggtca tcttcggttt ccgtgtttcg     6240

taaagtctgg aaacgcggaa gtcccctacg tgctgctgaa gttgcccgca acagagagtg     6300

gaaccaaccg gtgataccac gatactatga ctgagagtca acgccatgag cggcctcatt     6360

tcttattctg agttacaaca gtccgcaccg ctgccggtag ctccttccgg tgggcgcggg     6420

gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg     6480

tgccggcagc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac     6540

aagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg     6600

gaacacctac atctgtatta acgaagcgct aaccgttttt atcaggctct gggaggcaga     6660

ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca     6720

ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca     6780

atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct     6840

ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt     6900

taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac     6960

tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc     7020

tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa     7080

acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc     7140

cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg     7200

ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg     7260

tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa     7320

gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga     7380

tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt     7440

ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat     7500

tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg     7560

gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat     7620

aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt     7680

acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca     7740


<210>  55
<211>  7229
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-GNA1220 beta/alpha/beta plasmid

<400>  55
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atgatggtga     4380

accgaactgc cttctgcctt tctctgaccg ccgccctgat tctgaccgcc tgcagcagcg     4440

gagggggtgg tgaacaaatc aaccttgcca gtggtcagcg tgaagccgaa atccaacaat     4500

ccgaaggcga ggctcaggct gcggtcaatg cgtccaatgc cgagaaaatc gcccgcatca     4560

accgcgccaa aggcgaagcg gaatccctgc gccttgttgc cgaagccaat gccgaagcca     4620

tccgtcaaat tgccgccgcc cttcaaaccc aaggcggggc ggatgcggtc aatctgaaga     4680

ttgcggaaca atacgtagcc gcgttcaaca atcttgccaa agaaagcaat acgctgatta     4740

tgcccgccaa tgttgccgac atcggcagcc tgatttctgc cggcatgaaa attatcgaca     4800

gcagcaaaac cgccaaataa cctgcaggac tagtcggccg tacctgtcag accaagttta     4860

ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa     4920

gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc     4980

gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat     5040

ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga     5100

gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt     5160

tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata     5220

cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac     5280

cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg     5340

ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg     5400

tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag     5460

cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct     5520

ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc     5580

aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt     5640

ttgctggcct tttgctcaca tgtgggccct ttcgtctcgc gcgtttcggt gatagcttaa     5700

gtgcggtcat cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcccctacgt     5760

gctgctgaag ttgcccgcaa cagagagtgg aaccaaccgg tgataccacg atactatgac     5820

tgagagtcaa cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc     5880

tgccggtagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt     5940

cttctttatc atgcaactcg taggacaggt gccggcagcg cccaacagtc ccccggccac     6000

ggggcctgcc accataccca cgccgaaaca agcgccctgc accattatgt tccggatctg     6060

catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgcta     6120

accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat tattacctcc     6180

acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt cacactgctt     6240

ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta acgaccctgc     6300

cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc cgcttattat     6360

cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct taaaaaaatt     6420

acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat     6480

ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc     6540

cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca     6600

cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct     6660

caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat     6720

atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt     6780

cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac     6840

cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca agaatgtgaa     6900

taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag gccgtaatat     6960

ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc tcaaaatgtt     7020

ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt ttctccattt     7080

tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt agtgatctta     7140

tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat tttcgccaaa     7200

agttggccca gggcttcccg gtatcaaca                                       7229


<210>  56
<211>  8066
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pFP12-NHba plasmid

<400>  56
ctgcaggccg tctgaaccgc tctaaccgct ttttctcggc ttaatttttc tgtctctgtt       60

ataaaattgc tattcatctt gttcttcttc aaaaaaaagt taagtaaaat acctacctaa      120

atttttacta gttcgcaatc tacgagctta taacctcgtt ttttcaattc atttaaaaaa      180

tcagattttg agcctaattt tgatctattg ctatcgttac ccgctagaaa tacccagtaa      240

ttacgcaaat cttcattggt aactttcgta atatcggtgt aatgatcttc gagtattttt      300

aagcaatctc tagcccataa accgtactcg tgattgctca tcttagggtt ttgcttatcg      360

agtttgacga acttcccata cttgttttta tgtggaaata ctggccgttt tgcaacttct      420

tcaatttttt gagctgttcg ttttttacta ccaatcacaa aatttaaaga gtgaatagta      480

cgcccacgct tgatttgttc aacctcaacg actaaatcag atttctcgtt aatctcagtt      540

attgcaggtt ccaaaacacg ttgatttaat gaattaaatc tagggtattt attttcaacc      600

tgaagccatt cttttagttt ttctactgta atttcacgac taccaacaga gcgatattgt      660

gtaattagct cataaattcg aattgaatgt acactgttga aataagcgat atgtttgagt      720

tgatattgcg tgaattgccc tttaagttgc gttaggtatg gcataacttc atcagtcatt      780

gcaattctaa aacgcccctc tttcttgaaa tatgttctag aggaaaccca acgaaattca      840

gttacacggt ctttatcttc agttttaaca cttcggtcgt aaatccgttt tatagccgcc      900

tgaatttgct tataggcgtt atcttggctt atttctggaa actcacggac aaaatcagcc      960

accgtaaaat caaaaatctt ttgattagat ttcggatcca tagtcccgat agttaaagct     1020

aaaattctga tttcatcaat actcaatcgg taattggctt caataaggct attagccttt     1080

acaacaacta aatcatttgg cataagacaa caaatttcct gtttaaaaca acaagcaaaa     1140

tatacctgtt gtttatatat aaaacaacaa gtattttctt aaaagttgtc tataacagga     1200

aatttgttgt cgtataacag gaaatttgtt gtcttataac aggaaatttg ttgtcgtata     1260

acaggaaatt tgttgtcgta taagtttgta acttattgat tttactggtt ttaaaaacgc     1320

cgaaaacaag taaaaaacaa aaatataaaa atatagggac tttcgtccct tttttgggct     1380

ttcagcccta attttttctt ttttcaggat taaaaattac aaaaccctta cagagcaagt     1440

aaacttgttt gcttgttctg caagggttca gcaaccgtag ccgtcaggcg tagggcggta     1500

gcctataaaa gccatttaat tttatcttta aacttccttt taaatgcttt gagtgggtgt     1560

cttttatcgt actcatcaat ccttttttgc attctttcgt ttgctttgtg atcggcaaat     1620

tttgaataag atttttccat ctcatctaac attctatcaa tccgtttttt atgttgccat     1680

ttcaggtaaa cataaacact tatagcaatt aaagacaata tcaatacatt gtaaaaaatg     1740

attgttacaa tttcgctcac agttattttt tacctttttc aatttcttca ttgataaatg     1800

cactcaattc atcaaatttc ttgtcatcat tgataaattt acgcaactta gggaagtttc     1860

tatctacatc taaaagaggg ctatttatta cttcatttag ccaaaaagcc cctaataaaa     1920

ccttgtaatg cgtagctttc ttacgctttt ctgcttgttc ttttgactta atcgcacgaa     1980

ttttcgcttt gatttcgtcc tgcttgcgtt gtaaatctgc ttgttgctgt tccaatcttg     2040

taagtttttc gcttgccata ctagcccctt tatatagtta gaaattatcg ttattttatt     2100

cagtaggtgc taggcttgca agtgttctgt tcattacgtt aaaataacgt aatgcccact     2160

tatcagtttc tcttcgagaa actggtgggc aagcgtaccg cttgaccgtt tcgcaatact     2220

caacactatg gcaatctatc atttaaacgt tcgctattgc agtaaaagca aagggcaatc     2280

agctcaagcc aaaaacgact acatcaaccg caatgataaa tattcaaagc ggttagatga     2340

tttacagttt tcaggctatg gtaatatgcc aaaatttgcc gaagataatc cgcaagaatt     2400

ttggcgattg tcagatattt acgagcgagc taatgcccga gtttgtactg aaattgaatt     2460

tgctttacct agagaattaa ccctagaaca acagcaaaaa ttagtaagtt cgtttataga     2520

aaatacggtt gatagcggta gcaataaact accctactct ttcgctatcc ataccgataa     2580

aaataatcat aatccccatt gtcatttgat attttcagaa cgccaacttg acggcataga     2640

ccgtacagcc gagcagtttt ttaaacgtgc taatactaaa tccccagaaa agggcggagc     2700

gatgaaaacg gcagattttc gagatcgtga gtttatccaa tctgtccgaa aaacgtggag     2760

agagcaagct aatcaagcct tagagcaata cggatatgcc gcacgaattg acgaacgtag     2820

ctacaaggaa caaggcatag agcaagcccc aagagcaaga attgacaggg taacgtggca     2880

agaattgaac cgattagagc aagaagaacg ccaaatcgtg caagagcttg cacttaaagg     2940

acaagaaatt aacaaagaaa aatcctactt gcagaaaatc gaagaaaaac aggctcaagg     3000

aatgggcaaa tatgaatcca aattcgcagc tgcgttttct aaattatcgg aaagtgccct     3060

aaaacacgat ttaagcaacg aaaaagaaaa agacagtaaa atacacactc aagaagaaaa     3120

agtgcctcaa aatcgcattc aggggctttc tcaagcagat tttgatcagt ttttaattga     3180

tgaatggcta cctcaaatag aaaaatacgt taaagcccaa gaaaagcggg acggaatgga     3240

agtagagatc acgcaatacg acaaggattt acagcgtatt cagggagact ataacaagct     3300

cacagataaa aatcagggtt ttctcggttt atgggaaact aaagagcaaa aagcaaagaa     3360

aaaagagctt gaagatgaat acaaacatac agcagagcaa cggaacgcta aaagccaaga     3420

attagccgag tatagccaaa aaataaaagc atacgaacag aaaacgctag agccaatcaa     3480

cgagaagatt gccaaatatc aagctgacaa ccctgaaata aaaatgcgga gcttaggatt     3540

tgtgaaaaaa attaaggctc gaggggcata taaagcggct caagagcgaa tggagcgaga     3600

aaaacagcac caacaggaaa aacaacagag acatttagag cgagagagtg gtttgagctt     3660

gtagctaacg ccctacgcct acggcttcgg ttgttcaacc cttaaagaac tcgcaacaag     3720

ttgcaaattc tttaagggtt cgcaataaaa acaaccgcta aacatttctg cccagcggtt     3780

gaaaatttac ctattcacca ttacaatgat caagcaggaa atttttttga ttgccgtaaa     3840

tgtccgtata tctagttgag gcacaacccg ccaaagtcat tgccccaacc agaacggcga     3900

aagctaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac     3960

gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc     4020

actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt     4080

gtgagcggat aacaatttca cacgggaaac agctatgacc atgattacgc caagcttcct     4140

cagcgcatgc atccgggcag cagcgcatcg gctcgcacga ggtctgcgct tgaattgtgt     4200

tgtagaaaca caacgttttt gaaaaaataa gctattgttt tatatcaaaa tataatcatt     4260

tttaaaataa aggttgcggc atttatcaga tatttgttct gaaatgaaga cgtatcgggt     4320

gtttgcccga tgtttttagg tttttatcaa atttacaaaa ggaagcccat atgtttaaac     4380

gcagtgtgat tgcaatggct tgtatttttc ccctttcagc ctgtgggggc ggcggtggcg     4440

gatcgcccga tgtcaagtcg gcggacacgc cgtcaaaacc ggccgccccc gttgttgctg     4500

aaaatgccgg ggaaggggtg ctgccgaaag aaaagaaaga tgaggaggca gcgggcggtg     4560

cgccgcaagc cgatacgcag gacgcaaccg ccggagaagg cagccaagat atggcggcag     4620

tttcggcaga aaatacaggc aatggcggtg cggcaacaac ggacaacccc aaaaatgaag     4680

acgcgggggc gcaaaatgat atgccgcaaa atgccgccga atccgcaaat caaacaggga     4740

acaaccaacc cgccggttct tcagattccg cccccgcgtc aaaccctgcc cctgcgaatg     4800

gcggtagcga ttttggaagg acgaacgtgg gcaattctgt tgtgattgac ggaccgtcgc     4860

aaaatataac gttgacccac tgtaaaggcg atccttgtaa tggtgataat ttattggatg     4920

aagaagcacc gccaaaatca gaatttgaat cgttaagtga tgaagagaaa attaagaaat     4980

ataaaaaaga tggagaaaag tttactggtt tggttgctat aaaagttgaa aataatgggt     5040

tgaataaata taccattatt tatcaagctc agcctactcg ttctgcacgg tcgaggaggt     5100

cgcttccggc cgagattccg ctgattcccg tcaatcaggc cgatacgctg attgtggatg     5160

gggaagcggt cagcctgacg gggcattccg gcaatatctt cgcgcccgaa gggaattacc     5220

ggtatctgac ttacggggcg gaaaaattgc ccggcggatc gtatgccctc cgtgtgcaag     5280

gcgaaccggc aaaaggcgaa atgcttgcgg gcacggccgt gtacaacggc gaagtgctgc     5340

atttccacat ggaaaacggc cgtccgtacc cgtccggagg caggtttgcc gcaaaagtcg     5400

atttcggcag caaatctgtg gacggcatta tcgacagcag cgatgatttg cacatgggta     5460

cgcaaaaatt caaagccgcc atcgatggaa acggctttaa ggggacttgg acggaaaatg     5520

gcggcgggga tgtttccgga aggttttacg gcccggccgg cgaggaagtg gcgggaaaat     5580

acagctatcg cccgacagat gctgaaaagg gcggattcgg cgtgtttgcc ggcaaaaaag     5640

atcgggattg aaggcctcct gcaggactag tcggccgtac ctgtcagacc aagtttactc     5700

atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat     5760

cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc     5820

agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg     5880

ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct     5940

accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct     6000

tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct     6060

cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg     6120

gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc     6180

gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga     6240

gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg     6300

cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta     6360

tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg     6420

ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg     6480

ctggcctttt gctcacatgt gggccctttc gtctcgcgcg tttcggtgat agcttaagtg     6540

cggtcatctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc cctacgtgct     6600

gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata ctatgactga     6660

gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc gcaccgctgc     6720

cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt     6780

ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc cggccacggg     6840

gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc ggatctgcat     6900

cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctaacc     6960

gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat tacctccacg     7020

gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac actgcttccg     7080

gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg accctgccct     7140

gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc ttattatcac     7200

ttattcaggc gtagcaacca ggcgtttaag ggcaccaata actgccttaa aaaaattacg     7260

ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga     7320

agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt     7380

gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt     7440

ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa     7500

taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata     7560

tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag     7620

tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt     7680

ctttcattgc catacggaat tccggatgag cattcatcag gcgggcaaga atgtgaataa     7740

aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca     7800

gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt     7860

tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag     7920

cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt     7980

cattatggtg aaagttggaa cctcttacgt gccgatcaac gtctcatttt cgccaaaagt     8040

tggcccaggg cttcccggta tcaaca                                          8066


