                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>   BACTERIAL MICROCOMPARTMENT VIRUS-LIKE PARTICLES

<130>  SP102877WO

<150>  SG10202010547W
<151>  2020-10-23

<160>  121   

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S2CP amino acid sequence

<400>  1

Ser Lys Ile Thr Gly Ser Ser Gly Asn Asp Thr Gln Gly Ser Leu Ile 
1               5                   10                  15      


Thr Tyr Ser Gly Gly Ala Arg Gly 
            20                  


<210>  2
<211>  98
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CsoS1A amino acid sequence

<400>  2

Met Ala Asp Val Thr Gly Ile Ala Leu Gly Met Ile Glu Thr Arg Gly 
1               5                   10                  15      


Leu Val Pro Ala Ile Glu Ala Ala Asp Ala Met Thr Lys Ala Ala Glu 
            20                  25                  30          


Val Arg Leu Val Gly Arg Gln Phe Val Gly Gly Gly Tyr Val Thr Val 
        35                  40                  45              


Leu Val Arg Gly Glu Thr Gly Ala Val Asn Ala Ala Val Arg Ala Gly 
    50                  55                  60                  


Ala Asp Ala Cys Glu Arg Val Gly Asp Gly Leu Val Ala Ala His Ile 
65                  70                  75                  80  


Ile Ala Arg Val His Ser Glu Val Glu Asn Ile Leu Pro Lys Ala Pro 
                85                  90                  95      


Gln Ala 
        


<210>  3
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CsoS4A amino acid sequence

<400>  3

Met Lys Ile Met Gln Val Glu Lys Thr Leu Val Ser Thr Asn Arg Ile 
1               5                   10                  15      


Ala Asp Met Gly His Lys Pro Leu Leu Val Val Trp Glu Lys Pro Gly 
            20                  25                  30          


Ala Pro Arg Gln Val Ala Val Asp Ala Ile Gly Cys Ile Pro Gly Asp 
        35                  40                  45              


Trp Val Leu Cys Val Gly Ser Ser Ala Ala Arg Glu Ala Ala Gly Ser 
    50                  55                  60                  


Lys Ser Tyr Pro Ser Asp Leu Thr Ile Ile Gly Ile Ile Asp Gln Trp 
65                  70                  75                  80  


Asn Gly Glu 
            


<210>  4
<211>  99
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HO-H amino acid sequence

<400>  4

Met Ala Asp Ala Leu Gly Met Ile Glu Val Arg Gly Phe Val Gly Met 
1               5                   10                  15      


Val Glu Ala Ala Asp Ala Met Val Lys Ala Ala Lys Val Glu Leu Ile 
            20                  25                  30          


Gly Tyr Glu Lys Thr Gly Gly Gly Tyr Val Thr Ala Val Val Arg Gly 
        35                  40                  45              


Asp Val Ala Ala Val Lys Ala Ala Thr Glu Ala Gly Gln Arg Ala Ala 
    50                  55                  60                  


Glu Arg Val Gly Glu Val Val Ala Val His Val Ile Pro Arg Pro His 
65                  70                  75                  80  


Val Asn Val Asp Ala Ala Leu Pro Leu Gly Arg Thr Pro Gly Met Asp 
                85                  90                  95      


Lys Ser Ala 
            


<210>  5
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HO-P amino acid sequence

<400>  5

Met Val Leu Gly Lys Val Val Gly Thr Val Val Ala Ser Arg Lys Glu 
1               5                   10                  15      


Pro Arg Ile Glu Gly Leu Ser Leu Leu Leu Val Arg Ala Cys Asp Pro 
            20                  25                  30          


Asp Gly Thr Pro Thr Gly Gly Ala Val Val Cys Ala Asp Ala Val Gly 
        35                  40                  45              


Ala Gly Val Gly Glu Val Val Leu Tyr Ala Ser Gly Ser Ser Ala Arg 
    50                  55                  60                  


Gln Thr Glu Val Thr Asn Asn Arg Pro Val Asp Ala Thr Ile Met Ala 
65                  70                  75                  80  


Ile Val Asp Leu Val Glu Met Gly Gly Asp Val Arg Phe Arg Lys Asp 
                85                  90                  95      


<210>  6
<211>  205
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HO-T1 amino acid sequence

<400>  6

Met Asp His Ala Pro Glu Arg Phe Asp Ala Thr Pro Pro Ala Gly Glu 
1               5                   10                  15      


Pro Asp Arg Pro Ala Leu Gly Val Leu Glu Leu Thr Ser Ile Ala Arg 
            20                  25                  30          


Gly Ile Thr Val Ala Asp Ala Ala Leu Lys Arg Ala Pro Ser Leu Leu 
        35                  40                  45              


Leu Met Ser Arg Pro Val Ser Ser Gly Lys His Leu Leu Met Met Arg 
    50                  55                  60                  


Gly Gln Val Ala Glu Val Glu Glu Ser Met Ile Ala Ala Arg Glu Ile 
65                  70                  75                  80  


Ala Gly Ala Gly Ser Gly Ala Leu Leu Asp Glu Leu Glu Leu Pro Tyr 
                85                  90                  95      


Ala His Glu Gln Leu Trp Arg Phe Leu Asp Ala Pro Val Val Ala Asp 
            100                 105                 110         


Ala Trp Glu Glu Asp Thr Glu Ser Val Ile Ile Val Glu Thr Ala Thr 
        115                 120                 125             


Val Cys Ala Ala Ile Asp Ser Ala Asp Ala Ala Leu Lys Thr Ala Pro 
    130                 135                 140                 


Val Val Leu Arg Asp Met Arg Leu Ala Ile Gly Ile Ala Gly Lys Ala 
145                 150                 155                 160 


Phe Phe Thr Leu Thr Gly Glu Leu Ala Asp Val Glu Ala Ala Ala Glu 
                165                 170                 175     


Val Val Arg Glu Arg Cys Gly Ala Arg Leu Leu Glu Leu Ala Cys Ile 
            180                 185                 190         


Ala Arg Pro Val Asp Glu Leu Arg Gly Arg Leu Phe Phe 
        195                 200                 205 


<210>  7
<211>  72
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2CP nucleotide sequence

<400>  7
tctaagatta ctggttcttc tggtaacgat acccaaggtt ctttgattac ttactctggt       60

ggtgctagag gt                                                           72


<210>  8
<211>  294
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsoS1A nucleotide sequence

<400>  8
atggctgatg ttactggtat tgctttgggt atgattgaaa ctagaggttt ggttccagct       60

atcgaagctg ctgacgctat gaccaaggcc gctgaagtca gattggtcgg tagacaattt      120

gttggaggtg gttacgtcac tgttttggtt cgtggtgaaa ccggtgccgt taacgctgct      180

gttagagctg gtgctgatgc ttgtgaaaga gttggtgacg gtttagttgc tgcccacatt      240

attgccagag tccactctga agttgaaaac attttgccaa aggctccaca ggct            294


<210>  9
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CsoS4A nucleotide sequence

<400>  9
atgaagatca tgcaagttga aaagactttg gtttctacca acagaattgc tgatatgggt       60

cacaagccat tgttggttgt ttgggaaaaa cctggtgctc caagacaagt tgctgttgat      120

gctattggtt gtattccagg tgactgggtt ttgtgtgttg gttcttctgc tgccagagaa      180

gctgctggtt ccaagtctta cccatctgat ttgactatca tcggtattat tgaccaatgg      240

aacggtgaa                                                              249


<210>  10
<211>  297
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HO-H nuxleotide sequence

<400>  10
atggctgatg ctttgggtat gattgaagtt agaggtttcg ttggtatggt tgaagctgct       60

gatgctatgg ttaaggctgc taaagttgaa ttgatcggtt acgaaaaaac tggtggtggt      120

tatgttactg ctgttgttag aggtgatgtt gctgctgtaa aagctgctac tgaagctggt      180

caaagggctg ctgaaagagt tggagaagtt gttgctgttc atgttattcc aagaccacat      240

gttaatgttg atgctgcttt gccattgggt agaactccag gtatggataa gtctgct         297


<210>  11
<211>  288
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HO-P nucleotide sequence

<400>  11
atggttttag gtaaagttgt cggtactgtt gttgcatcaa gaaaggaacc aagaattgaa       60

ggtttatctt tattattggt tagagcttgt gatccagatg gtactccaac tggtggtgct      120

gttgtttgtg ctgatgctgt tggtgctggt gttggtgaag ttgttttata tgcttctggt      180

tcttctgcta gacaaactga agttactaat aatagaccag ttgatgctac tattatggct      240

attgttgatt tggttgaaat gggtggtgat gttagattta gaaaagat                   288


<210>  12
<211>  615
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HO-T1 nucleotide sequence

<400>  12
atggatcatg ctccagaaag atttgatgct actcctccag ctggtgaacc agatagacca       60

gctttgggtg ttttggaatt gacttctatt gctagaggta ttaccgttgc tgatgctgct      120

ttgaaaagag caccatcttt gttgttgatg tccagaccag tttcttccgg taaacatttg      180

ttgatgatga gaggtcaagt tgccgaagtt gaagaatcta tgattgctgc tagagaaatt      240

gctggtgctg gttctggtgc tttgttggat gaattggaat tgccatatgc tcacgaacaa      300

ctttggagat ttttggatgc tccagttgtt gcagatgctt gggaagaaga tactgaatcc      360

gttattatcg ttgaaaccgc tactgtttgt gctgctattg attctgctga tgcagcctta      420

aaaactgctc ctgttgtttt gagagatatg agattggcta ttggtattgc tggtaaggct      480

ttctttactt tgactggtga attggctgat gttgaagctg ctgctgaagt tgttagagaa      540

agatgtggtg ctagattgct agaattggct tgtattgcaa gaccagttga cgaattgaga      600

ggtaggttgt ttttc                                                       615


<210>  13
<211>  83
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spycatcher tag amino acid sequence

<400>  13

Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
1               5                   10                  15      


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
            20                  25                  30          


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
        35                  40                  45              


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
    50                  55                  60                  


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
65                  70                  75                  80  


Val Asn Gly 
            


<210>  14
<211>  249
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spycatcher tag nucleotide sequence

<400>  14
gattctgcta ctcatattaa gttctccaag agggacgaag atggtaaaga attggctggt       60

gcaactatgg aattgagaga ttcttctggt aagaccattt ccacctggat ttctgatggt      120

caagttaagg atttctactt gtacccaggt aagtacactt tcgttgaaac tgctgctcca      180

gatggttatg aagttgctac tgctattact ttcaccgtca atgaacaagg tcaagtcact      240

gttaatggt                                                              249


<210>  15
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Spytag amino acid sequence

<400>  15

Ala His Ile Val Met Val Asp Ala Tyr Lys Pro Thr Lys 
1               5                   10              


<210>  16
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Spytag nucleotide sequence

<400>  16
gctcatatag ttatggttga tgcttacaag ccaacaaaa                              39


<210>  17
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CC-Di-A amino acid sequence

<400>  17

Glu Ile Ala Ala Leu Glu Lys Glu Asn Ala Ala Leu Glu Gln Glu Ile 
1               5                   10                  15      


Ala Ala Leu Glu Gln 
            20      


<210>  18
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CC-Di-A nucleotide sequence

<400>  18
gaaattgcag ctttggaaaa agaaaacgct gccttggaac aagaaattgc cgcattagaa       60

caa                                                                     63


<210>  19
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CC-Di-B amino acid sequemce

<400>  19

Lys Ile Ala Ala Leu Lys Lys Lys Asn Ala Ala Leu Lys Gln Lys Ile 
1               5                   10                  15      


Ala Ala Leu Lys Gln 
            20      


<210>  20
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CC-Di-B nucleotide sequence

<400>  20
aaaattgcag cattgaaaaa gaagaacgcc gccttgaaac aaaaaattgc tgccttaaaa       60

caa                                                                     63


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Strep-Tag II (SII) amino acid sequence

<400>  21

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  22
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Strep-Tag II (SII) nucleotide sequence

<400>  22
tggtcacatc cacaatttga aaag                                              24


<210>  23
<211>  1555
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PT7 nucleotide sequence

<400>  23
tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa       60

cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga      120

aacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc      180

cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata      240

acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag      300

cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat      360

cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc      420

actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg      480

ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat      540

ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga      600

gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt      660

agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag      720

cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct      780

tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc      840

cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa      900

cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat      960

cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg     1020

ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt     1080

cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt     1140

tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta     1200

ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat     1260

gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga     1320

aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga     1380

tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt     1440

agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc     1500

ggataacaat tcccctctag aaataatttt gtttaacttt aagaaggaga tatac          1555


<210>  24
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PCON5 nucleotide sequence

<400>  24
ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccgacgtctt tatggctagc       60

tcagtcctag gtacaatgct agcgaattca aaagatcttt taagaaggag atatacat        118


<210>  25
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PTDH3 nucleotide sequence

<400>  25
acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag       60

aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt      120

ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac      180

ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc      240

atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa      300

agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt      360

atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat      420

tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata      480

aacacacata aacaaacaaa                                                  500


<210>  26
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PPYK1 nucleotide sequence

<400>  26
acagattggg agattttcat agtagaattc agcatgatag ctacgtaaat gtgttccgca       60

ccgtcacaaa gtgttttcta ctgttctttc ttctttcgtt cattcagttg agttgagtga      120

gtgctttgtt caatggatct tagctaaaat gcatattttt tctcttggta aatgaatgct      180

tgtgatgtct tccaagtgat ttcctttcct tcccatatga tgctaggtac ctttagtgtc      240

ttcctaaaaa aaaaaaaagg ctcgccatca aaacgatatt cgttggcttt tttttctgaa      300

ttataaatac tctttggtaa cttttcattt ccaagaacct cttttttcca gttatatcat      360

ggtccccttt caaagttatt ctctactctt tttcatattc attctttttc atcctttggt      420

tttttattct taacttgttt attattctct cttgtttcta tttacaagac accaatcaaa      480

acaaataaaa catcatcaca                                                  500


<210>  27
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PYEF3 nucleotide sequence

<400>  27
attaaaaaaa caacttacaa tcattgttcg ccccttccat acttactgcc actcgcaaaa       60

gggcccaacc agggcaatta cgtatcaaaa aatcatgaca ggctgggtaa taaatattcg      120

tgaagaaaga agaaattaaa aaaagaaacg aagaagcaaa aaaaagaaaa gactccgttt      180

aatcactttc aaccgcggtt tatccggccc cacccatgca taaccctaaa ttattagatc      240

acttagcacg tgaaaaagaa acgtttttaa tgtttttttt ttttttttct ttttcttttt      300

ttgcgttggt gaaaattttt tcgcttcctc gagtataatt atctcatctc atctttcata      360

taagataaga agttttataa aaaccttttg catcaaaatt ttgtagaata tctctttttc      420

ttacgctctc tttctttcct taattgtttt ctaaagaacc gtgtattttt ctagttcgaa      480

tccatcgata acattaaaag                                                  500


<210>  28
<211>  3589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CmCherry plasmid

<400>  28
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttcttctat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc     1260

gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg     1320

gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc     1380

ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg     1440

tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt     1500

gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc     1560

tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg     1620

gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg     1680

aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact     1740

acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct     1800

acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac     1860

agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagc     1920

cgagacgact gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg     1980

accggaggct tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata     2040

agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa     2100

atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat     2160

gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc     2220

tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc     2280

gttgccaatg atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct     2340

cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg     2400

atcccaggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt     2460

gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct     2520

tttaacggcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg     2580

gttggtgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa     2640

gaaatgcata agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca     2700

cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc     2760

ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct     2820

ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa     2880

ttgcagtttc acttgatgct cgatgagttt ttctaatgag ggcccaaatg taatcacctg     2940

gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct     3000

gacgagcatc acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa     3060

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg     3120

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca     3180

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa     3240

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg     3300

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg     3360

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga     3420

acagtatttg gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct     3480

cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga     3540

ttacgcgcag aaaaaaagga tctcaagaag atcctttgat tttctaccg                 3589


<210>  29
<211>  708
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CmCherry nucleotide sequence of key ORF

<400>  29
atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag       60

gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc      120

cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc      180

ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac      240

cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc      300

gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac      360

ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta      420

atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc      480

gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct      540

gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc      600

aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa      660

cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaag                   708


<210>  30
<211>  236
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CmCherry amino acid sequence of key ORF

<400>  30

Met Val Ser Lys Gly Glu Glu Asp Asn Met Ala Ile Ile Lys Glu Phe 
1               5                   10                  15      


Met Arg Phe Lys Val His Met Glu Gly Ser Val Asn Gly His Glu Phe 
            20                  25                  30          


Glu Ile Glu Gly Glu Gly Glu Gly Arg Pro Tyr Glu Gly Thr Gln Thr 
        35                  40                  45              


Ala Lys Leu Lys Val Thr Lys Gly Gly Pro Leu Pro Phe Ala Trp Asp 
    50                  55                  60                  


Ile Leu Ser Pro Gln Phe Met Tyr Gly Ser Lys Ala Tyr Val Lys His 
65                  70                  75                  80  


Pro Ala Asp Ile Pro Asp Tyr Leu Lys Leu Ser Phe Pro Glu Gly Phe 
                85                  90                  95      


Lys Trp Glu Arg Val Met Asn Phe Glu Asp Gly Gly Val Val Thr Val 
            100                 105                 110         


Thr Gln Asp Ser Ser Leu Gln Asp Gly Glu Phe Ile Tyr Lys Val Lys 
        115                 120                 125             


Leu Arg Gly Thr Asn Phe Pro Ser Asp Gly Pro Val Met Gln Lys Lys 
    130                 135                 140                 


Thr Met Gly Trp Glu Ala Ser Ser Glu Arg Met Tyr Pro Glu Asp Gly 
145                 150                 155                 160 


Ala Leu Lys Gly Glu Ile Lys Gln Arg Leu Lys Leu Lys Asp Gly Gly 
                165                 170                 175     


His Tyr Asp Ala Glu Val Lys Thr Thr Tyr Lys Ala Lys Lys Pro Val 
            180                 185                 190         


Gln Leu Pro Gly Ala Tyr Asn Val Asn Ile Lys Leu Asp Ile Thr Ser 
        195                 200                 205             


His Asn Glu Asp Tyr Thr Ile Val Glu Gln Tyr Glu Arg Ala Glu Gly 
    210                 215                 220                 


Arg His Ser Thr Gly Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235     


<210>  31
<211>  2905
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CSII plasmid

<400>  31
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttcttcttg gtcacatcca caatttgaaa agtagccgag acgactgacc atttaaatca     1260

tacctgacct ccatagcaga aagtcaaaag cctccgaccg gaggcttttg acttgatcgg     1320

cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt     1380

gagttatcga gattttcagg agctaaggaa gctaaaatga gccatattca acgggaaacg     1440

tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg     1500

gctcgcgata atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat     1560

gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag     1620

atggtcaggc taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc     1680

cgtactcctg atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag     1740

gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg     1800

cgccggttgc attcgattcc tgtttgtaat tgtcctttta acggcgatcg cgtatttcgt     1860

ctcgctcagg cgcaatcacg aatgaataac ggtttggttg gtgcgagtga ttttgatgac     1920

gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc     1980

tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag     2040

gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat     2100

cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt     2160

caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcactt gatgctcgat     2220

gagtttttct aatgagggcc caaatgtaat cacctggctc accttcgggt gggcctttct     2280

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgatgc     2340

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga     2400

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt     2460

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg     2520

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc     2580

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg     2640

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc     2700

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg     2760

ctgaagccag ttacctcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg     2820

ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc     2880

aagaagatcc tttgattttc taccg                                           2905


<210>  32
<211>  2899
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CHis6 plasmid

<400>  32
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttcttctca tcatcaccat caccattagc cgagacgact gaccatttaa atcatacctg     1260

acctccatag cagaaagtca aaagcctccg accggaggct tttgacttga tcggcacgta     1320

agaggttcca actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta     1380

tcgagatttt caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc     1440

tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc     1500

gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca     1560

gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc     1620

aggctaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact     1680

cctgatgatg catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta     1740

gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg     1800

ttgcattcga ttcctgtttg taattgtcct tttaacggcg atcgcgtatt tcgtctcgct     1860

caggcgcaat cacgaatgaa taacggtttg gttggtgcga gtgattttga tgacgagcgt     1920

aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg     1980

gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa     2040

ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc     2100

atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa     2160

tatggtattg ataatcctga tatgaataaa ttgcagtttc acttgatgct cgatgagttt     2220

ttctaatgag ggcccaaatg taatcacctg gctcaccttc gggtgggcct ttctgcgttg     2280

ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg atgctcaagt     2340

cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc     2400

ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct     2460

tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc     2520

gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta     2580

tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca     2640

gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag     2700

tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag     2760

ccagttacct cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta     2820

gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag     2880

atcctttgat tttctaccg                                                  2899


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CHis6 nucleotide sequence of key ORF

<400>  33
catcatcacc atcaccat                                                     18


<210>  34
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CHis6 amino acid sequence of key ORF

<400>  34

His His His His His His 
1               5       


<210>  35
<211>  2983
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CCCDiA plasmid

<400>  35
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttctggtgg tggttcaggt ggttctgaaa ttgcagcttt ggaaaaagaa aacgctgcct     1260

tggaacaaga aattgccgca ttagaacaag gtggtagtgg tggatctggt tagccgagac     1320

gactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga     1380

ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca     1440

ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc     1500

catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat     1560

ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga     1620

ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc     1680

aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg     1740

accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca     1800

gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat     1860

gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac     1920

ggcgatcgcg tatttcgtct cgcacaggcg caatcacgaa tgaataacgg tttggttggt     1980

gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg     2040

cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat     2100

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc     2160

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca     2220

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag     2280

tttcacttga tgctcgatga gtttttctaa tgagggccca aatgtaatca cctggctcac     2340

cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag     2400

catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     2460

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     2520

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     2580

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     2640

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     2700

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     2760

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta     2820

tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat     2880

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     2940

gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccg                       2983


<210>  36
<211>  2983
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CCCDiB plasmid

<400>  36
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttctggtgg tggtagtggt ggttctaaaa ttgcagcatt gaaaaagaag aacgccgcct     1260

tgaaacaaaa aattgctgcc ttaaaacaag gtggtagtgg tggatctggt tagccgagac     1320

gactgaccat ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga     1380

ggcttttgac ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca     1440

ctaccgggcg tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc     1500

catattcaac gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat     1560

ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga     1620

ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc     1680

aatgatgtta cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg     1740

accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca     1800

gggaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat     1860

gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac     1920

ggcgatcgcg tatttcgtct cgcacaggcg caatcacgaa tgaataacgg tttggttggt     1980

gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg     2040

cataagcttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat     2100

aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc     2160

gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca     2220

ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag     2280

tttcacttga tgctcgatga gtttttctaa tgagggccca aatgtaatca cctggctcac     2340

cttcgggtgg gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag     2400

catcacaaaa atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac     2460

caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc     2520

ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt     2580

aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc     2640

gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga     2700

cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta     2760

ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta     2820

tttggtatct gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat     2880

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     2940

gcagaaaaaa aggatctcaa gaagatcctt tgattttcta ccg                       2983


<210>  37
<211>  3136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CSpyCatcher plasmid

<400>  37
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttctggtgg ttctgattct gctactcata ttaagttctc caagagggac gaagatggta     1260

aagaattggc tggtgcaact atggaattga gagattcttc tggtaagacc atttccacct     1320

ggatttctga tggtcaagtt aaggatttct acttgtaccc aggtaagtac actttcgttg     1380

aaactgctgc tccagatggt tatgaagttg ctactgctat tactttcacc gtcaatgaac     1440

aaggtcaagt cactgttaat ggttagccga gacgactgac catttaaatc atacctgacc     1500

tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga     1560

ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg     1620

agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg     1680

aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat     1740

aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag     1800

ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg     1860

ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct     1920

gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa     1980

gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg     2040

cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag     2100

gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat     2160

ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat     2220

tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta     2280

ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc     2340

ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat     2400

ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc     2460

taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg     2520

gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag     2580

aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc     2640

gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg     2700

ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt     2760

cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc     2820

ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc     2880

actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg     2940

tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca     3000

gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg     3060

gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc     3120

ctttgatttt ctaccg                                                     3136


<210>  38
<211>  2926
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CSpyTag plasmid

<400>  38
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttctggtgg ttctgctcat atagttatgg ttgatgctta caagccaaca aaatagccga     1260

gacgactgac catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc     1320

ggaggctttt gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga     1380

tcactaccgg gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg     1440

agccatattc aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct     1500

gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat     1560

cgattgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt     1620

gccaatgatg ttacagatga gatggtcagg ctaaactggc tgacggaatt tatgcctctt     1680

ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc     1740

ccagggaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt     1800

gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt     1860

aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt     1920

ggtgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa     1980

atgcataagc ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt     2040

gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga     2100

atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct     2160

tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg     2220

cagtttcact tgatgctcga tgagtttttc taatgagggc ccaaatgtaa tcacctggct     2280

caccttcggg tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac     2340

gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga     2400

taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt     2460

accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc     2520

tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc     2580

cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta     2640

agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat     2700

gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca     2760

gtatttggta tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt     2820

gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta     2880

cgcgcagaaa aaaaggatct caagaagatc ctttgatttt ctaccg                    2926


<210>  39
<211>  2944
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-S2CP plasmid

<400>  39
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctt     1200

ctaagattac tggttcttct ggtaacgata cccaaggttc tttgattact tactctggtg     1260

gtgctagagg ttagccgaga cgactgacca tttaaatcat acctgacctc catagcagaa     1320

agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt     1380

caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga     1440

gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta     1500

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa     1560

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa     1620

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg     1680

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg     1740

ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat     1800

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct     1860

gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga     1920

atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt     1980

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact     2040

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     2100

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     2160

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     2220

cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc     2280

aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat     2340

aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac     2400

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     2460

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     2520

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     2580

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     2640

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     2700

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     2760

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa     2820

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     2880

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct     2940

accg                                                                  2944


<210>  40
<211>  1864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-Pcon3 plasmid

<400>  40
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt      120

cctgacagct agctcagtcc taggtataat gctagcgaat tcaaaagatc ttttaagaag      180

gagatataca tgatgcgaga cgactgacca tttaaatcat acctgacctc catagcagaa      240

agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt      300

caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga      360

gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta      420

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa      480

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa      540

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg      600

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg      660

ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat      720

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct      780

gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga      840

atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt      900

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact      960

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1020

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1080

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1140

cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc     1200

aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat     1260

aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac     1320

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     1380

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     1440

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     1500

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     1560

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     1620

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     1680

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa     1740

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     1800

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct     1860

accg                                                                  1864


<210>  41
<211>  1864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-Pcon4 plasmid

<400>  41
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt      120

ctttacggct agctcagtcc taggtactat gctagcgaat tcaaaagatc ttttaagaag      180

gagatataca tgatgcgaga cgactgacca tttaaatcat acctgacctc catagcagaa      240

agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt      300

caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga      360

gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta      420

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa      480

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa      540

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg      600

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg      660

ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat      720

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct      780

gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga      840

atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt      900

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact      960

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1020

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1080

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1140

cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc     1200

aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat     1260

aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac     1320

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     1380

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     1440

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     1500

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     1560

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     1620

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     1680

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa     1740

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     1800

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct     1860

accg                                                                  1864


<210>  42
<211>  1864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-Pcon5 plasmid

<400>  42
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt      120

ctttatggct agctcagtcc taggtacaat gctagcgaat tcaaaagatc ttttaagaag      180

gagatataca tgatgcgaga cgactgacca tttaaatcat acctgacctc catagcagaa      240

agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt      300

caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga      360

gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta      420

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa      480

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa      540

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg      600

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg      660

ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat      720

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct      780

gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga      840

atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt      900

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact      960

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1020

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1080

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1140

cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc     1200

aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat     1260

aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac     1320

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     1380

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     1440

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     1500

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     1560

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     1620

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     1680

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa     1740

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     1800

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct     1860

accg                                                                  1864


<210>  43
<211>  3301
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-LacI+PT7 plasmid

<400>  43
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gcttcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta      120

atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc      180

ttttcaccag tgaaacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt      240

gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta      300

acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg      360

caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt      420

tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa      480

aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag      540

tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg      600

ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac      660

cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata      720

acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat      780

agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg      840

cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc      900

gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa      960

cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat     1020

tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct     1080

ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata     1140

acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca     1200

taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc     1260

gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca     1320

aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc     1380

atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg     1440

gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg     1500

atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag     1560

gggaattgtg agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg     1620

agatatacga tgcgagacga ctgaccattt aaatcatacc tgacctccat agcagaaagt     1680

caaaagcctc cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac     1740

cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct     1800

aaggaagcta aaatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat     1860

tccaacatgg atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca     1920

ggtgcgacaa tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat     1980

ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg     2040

gaatttatgc ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta     2100

ctcaccactg cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca     2160

ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt     2220

tgtaattgtc cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg     2280

aataacggtt tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa     2340

caagtctgga aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat     2400

ggtgatttct cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat     2460

gttggacgag tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc     2520

ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct     2580

gatatgaata aattgcagtt tcacttgatg ctcgatgagt ttttctaatg agggcccaaa     2640

tgtaatcacc tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg     2700

ctccgccccc ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg     2760

acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt     2820

ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt     2880

tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc     2940

tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt     3000

gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt     3060

agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc     3120

tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa     3180

gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt     3240

gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc     3300

g                                                                     3301


<210>  44
<211>  2499
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-His6-meGFP plasmid

<400>  44
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgggttctt ctcatcatca ccatcaccat tcttctggga tgtctaaagg      120

tgaagaatta ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg      180

tcacaaattt tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt      240

aaaatttatt tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt      300

aacttatggt gttcaatgtt tttctagata cccagatcat atgaaacaac atgacttttt      360

caagtctgcc atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg      420

taactacaag accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga      480

attaaaaggt attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa      540

ctataactct cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa      600

cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca      660

aaatactcca attggtgatg gtccagtctt gttaccagac aaccattact tatccactca      720

atctaaatta tccaaagatc caaacgaaaa gagagatcac atggtcttgt tagaatttgt      780

tactgctgct ggtattaccc atggtatgga tgaattgtac aaataatagc cgagacgact      840

gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct      900

tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac      960

cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata     1020

ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat     1080

atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt     1140

atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg     1200

atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca     1260

tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga     1320

aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc     1380

tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg     1440

atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga     1500

gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata     1560

agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc     1620

ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag     1680

accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac     1740

agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc     1800

acttgatgct cgatgagttt ttctaatgag ggcccaaatg taatcacctg gctcaccttc     1860

gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     1920

acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     1980

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     2040

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     2100

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     2160

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     2220

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     2280

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg     2340

gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg     2400

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     2460

aaaaaaagga tctcaagaag atcctttgat tttctaccg                            2499


<210>  45
<211>  753
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-His6-meGFP nucleotide sequence of key ORF

<400>  45
atgggttctt ctcatcatca ccatcaccat tcttctggga tgtctaaagg tgaagaatta       60

ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt      120

tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt      180

tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt aacttatggt      240

gttcaatgtt tttctagata cccagatcat atgaaacaac atgacttttt caagtctgcc      300

atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg taactacaag      360

accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga attaaaaggt      420

attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa ctataactct      480

cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt      540

agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca aaatactcca      600

attggtgatg gtccagtctt gttaccagac aaccattact tatccactca atctaaatta      660

tccaaagatc caaacgaaaa gagagatcac atggtcttgt tagaatttgt tactgctgct      720

ggtattaccc atggtatgga tgaattgtac aaa                                   753


<210>  46
<211>  251
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-His6-meGFP amino acid sequence of key ORF

<400>  46

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Ser Lys 
1               5                   10                  15      


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
            20                  25                  30          


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
        35                  40                  45              


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
    50                  55                  60                  


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
65                  70                  75                  80  


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
                85                  90                  95      


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
            100                 105                 110         


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
        115                 120                 125             


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
    130                 135                 140                 


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
145                 150                 155                 160 


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
                165                 170                 175     


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
            180                 185                 190         


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        195                 200                 205             


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro 
    210                 215                 220                 


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
225                 230                 235                 240 


Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
                245                 250     


<210>  47
<211>  2658
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP-S2CP plasmid

<400>  47
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgctgttta tcaaacctgc cgatctgcgt gaaattgtta cctttccgct      120

gtttagcgat ctggttcagt gtggttttcc gagtccggca gcagattatg ttgaacagcg      180

tattgatctg agcagcggta tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat      240

tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga      300

aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc      360

agttccatgg ccaaccttag tcactacttt aacttatggt gttcaatgtt tttctagata      420

cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca      480

agaaagaact atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt      540

tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg      600

taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc      660

tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg      720

ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt      780

gttaccagac aaccattact tatccactca atctaaatta tccaaagatc caaacgaaaa      840

gagagatcac atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga      900

tgaattgtac aaatctaaga ttactggttc ttctggtaac gatacccaag gttctttgat      960

tacttactct ggtggtgcta gaggttagcc gagacgactg accatttaaa tcatacctga     1020

cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa     1080

gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat     1140

cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct     1200

cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg     1260

ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag     1320

agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca     1380

ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc     1440

ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag     1500

aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt     1560

tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc     1620

aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta     1680

atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg     1740

attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat     1800

taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca     1860

tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat     1920

atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt     1980

tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc     2040

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc     2100

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     2160

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     2220

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     2280

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     2340

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     2400

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     2460

ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc     2520

cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     2580

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     2640

tcctttgatt ttctaccg                                                   2658


<210>  48
<211>  915
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP-S2CP nucl;eotide sequence of key ORF

<400>  48
atgctgttta tcaaacctgc cgatctgcgt gaaattgtta cctttccgct gtttagcgat       60

ctggttcagt gtggttttcc gagtccggca gcagattatg ttgaacagcg tattgatctg      120

agcagcggta tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa      180

ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct      240

acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg      300

ccaaccttag tcactacttt aacttatggt gttcaatgtt tttctagata cccagatcat      360

atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact      420

atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat      480

accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta      540

ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa      600

aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa      660

ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac      720

aaccattact tatccactca atctaaatta tccaaagatc caaacgaaaa gagagatcac      780

atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac      840

aaatctaaga ttactggttc ttctggtaac gatacccaag gttctttgat tacttactct      900

ggtggtgcta gaggt                                                       915


<210>  49
<211>  305
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP-S2CP amino acid sequence of key ORF

<400>  49

Met Leu Phe Ile Lys Pro Ala Asp Leu Arg Glu Ile Val Thr Phe Pro 
1               5                   10                  15      


Leu Phe Ser Asp Leu Val Gln Cys Gly Phe Pro Ser Pro Ala Ala Asp 
            20                  25                  30          


Tyr Val Glu Gln Arg Ile Asp Leu Ser Ser Gly Met Ser Lys Gly Glu 
        35                  40                  45              


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
    50                  55                  60                  


Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
65                  70                  75                  80  


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
                85                  90                  95      


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln 
            100                 105                 110         


Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
        115                 120                 125             


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
    130                 135                 140                 


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
145                 150                 155                 160 


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
                165                 170                 175     


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
            180                 185                 190         


Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe 
        195                 200                 205             


Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
    210                 215                 220                 


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
225                 230                 235                 240 


Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu 
                245                 250                 255     


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 
            260                 265                 270         


Thr His Gly Met Asp Glu Leu Tyr Lys Ser Lys Ile Thr Gly Ser Ser 
        275                 280                 285             


Gly Asn Asp Thr Gln Gly Ser Leu Ile Thr Tyr Ser Gly Gly Ala Arg 
    290                 295                 300                 


Gly 
305 


<210>  50
<211>  2586
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP plasmid

<400>  50
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgctgttta tcaaacctgc cgatctgcgt gaaattgtta cctttccgct      120

gtttagcgat ctggttcagt gtggttttcc gagtccggca gcagattatg ttgaacagcg      180

tattgatctg agcagcggta tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat      240

tttggttgaa ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga      300

aggtgatgct acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc      360

agttccatgg ccaaccttag tcactacttt aacttatggt gttcaatgtt tttctagata      420

cccagatcat atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca      480

agaaagaact atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt      540

tgaaggtgat accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg      600

taacatttta ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc      660

tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg      720

ttctgttcaa ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt      780

gttaccagac aaccattact tatccactca atctaaatta tccaaagatc caaacgaaaa      840

gagagatcac atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga      900

tgaattgtac aaatagccga gacgactgac catttaaatc atacctgacc tccatagcag      960

aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg gcacgtaaga ggttccaact     1020

ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag     1080

gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg aggccgcgat     1140

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc     1200

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga     1260

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc     1320

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat     1380

ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg     1440

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc     1500

ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgctcag gcgcaatcac     1560

gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg     1620

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca     1680

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta     1740

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact     1800

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata     1860

atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc taatgagggc     1920

ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc     1980

ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa     2040

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     2100

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     2160

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     2220

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     2280

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     2340

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     2400

acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg     2460

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     2520

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt     2580

ctaccg                                                                2586


<210>  51
<211>  843
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP nucleotide sequence of key ORF

<400>  51
atgctgttta tcaaacctgc cgatctgcgt gaaattgtta cctttccgct gtttagcgat       60

ctggttcagt gtggttttcc gagtccggca gcagattatg ttgaacagcg tattgatctg      120

agcagcggta tgtctaaagg tgaagaatta ttcactggtg ttgtcccaat tttggttgaa      180

ttagatggtg atgttaatgg tcacaaattt tctgtctccg gtgaaggtga aggtgatgct      240

acttacggta aattgacctt aaaatttatt tgtactactg gtaaattgcc agttccatgg      300

ccaaccttag tcactacttt aacttatggt gttcaatgtt tttctagata cccagatcat      360

atgaaacaac atgacttttt caagtctgcc atgccagaag gttatgttca agaaagaact      420

atttttttca aagatgacgg taactacaag accagagctg aagtcaagtt tgaaggtgat      480

accttagtta atagaatcga attaaaaggt attgatttta aagaagatgg taacatttta      540

ggtcacaaat tggaatacaa ctataactct cacaatgttt acatcatggc tgacaaacaa      600

aagaatggta tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg ttctgttcaa      660

ttagctgacc attatcaaca aaatactcca attggtgatg gtccagtctt gttaccagac      720

aaccattact tatccactca atctaaatta tccaaagatc caaacgaaaa gagagatcac      780

atggtcttgt tagaatttgt tactgctgct ggtattaccc atggtatgga tgaattgtac      840

aaa                                                                    843


<210>  52
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-UmuD1-40-meGFP amino acid sequence of key ORF

<400>  52

Met Leu Phe Ile Lys Pro Ala Asp Leu Arg Glu Ile Val Thr Phe Pro 
1               5                   10                  15      


Leu Phe Ser Asp Leu Val Gln Cys Gly Phe Pro Ser Pro Ala Ala Asp 
            20                  25                  30          


Tyr Val Glu Gln Arg Ile Asp Leu Ser Ser Gly Met Ser Lys Gly Glu 
        35                  40                  45              


Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp 
    50                  55                  60                  


Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala 
65                  70                  75                  80  


Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu 
                85                  90                  95      


Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln 
            100                 105                 110         


Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys 
        115                 120                 125             


Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys 
    130                 135                 140                 


Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp 
145                 150                 155                 160 


Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp 
                165                 170                 175     


Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn 
            180                 185                 190         


Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val Asn Phe 
        195                 200                 205             


Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His 
    210                 215                 220                 


Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp 
225                 230                 235                 240 


Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu 
                245                 250                 255     


Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile 
            260                 265                 270         


Thr His Gly Met Asp Glu Leu Tyr Lys 
        275                 280     


<210>  53
<211>  2037
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CsoS1A plasmid

<400>  53
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atggctgatg ttactggtat tgctttgggt atgattgaaa ctagaggttt      120

ggttccagct atcgaagctg ctgacgctat gaccaaggcc gctgaagtca gattggtcgg      180

tagacaattt gttggaggtg gttacgtcac tgttttggtt cgtggtgaaa ccggtgccgt      240

taacgctgct gttagagctg gtgctgatgc ttgtgaaaga gttggtgacg gtttagttgc      300

tgcccacatt attgccagag tccactctga agttgaaaac attttgccaa aggctccaca      360

ggcttagccg agacgactga ccatttaaat catacctgac ctccatagca gaaagtcaaa      420

agcctccgac cggaggcttt tgacttgatc ggcacgtaag aggttccaac tttcaccata      480

atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg      540

aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga ttaaattcca      600

acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg      660

cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca      720

aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg ctgacggaat      780

ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca      840

ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg      900

aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta      960

attgtccttt taacggcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata     1020

acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag     1080

tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc actcatggtg     1140

atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg     1200

gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg     1260

agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata     1320

tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgaggg cccaaatgta     1380

atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc cataggctcc     1440

gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga aacccgacag     1500

gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga     1560

ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc     1620

atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg     1680

tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt     1740

ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca     1800

gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca     1860

ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg gaaaaagagt     1920

tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa     1980

gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt tctaccg        2037


<210>  54
<211>  1992
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-CsoS4A plasmid

<400>  54
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgaagatca tgcaagttga aaagactttg gtttctacca acagaattgc      120

tgatatgggt cacaagccat tgttggttgt ttgggaaaaa cctggtgctc caagacaagt      180

tgctgttgat gctattggtt gtattccagg tgactgggtt ttgtgtgttg gttcttctgc      240

tgccagagaa gctgctggtt ccaagtctta cccatctgat ttgactatca tcggtattat      300

tgaccaatgg aacggtgaat agccgagacg actgaccatt taaatcatac ctgacctcca      360

tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgatcggcac gtaagaggtt      420

ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat      480

tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc      540

cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg      600

tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt      660

ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcaggctaa      720

actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg      780

atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat      840

atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt      900

cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc      960

aatcacgaat gaataacggt ttggttggtg cgagtgattt tgatgacgag cgtaatggct     1020

ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag     1080

tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag     1140

gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat     1200

ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta     1260

ttgataatcc tgatatgaat aaattgcagt ttcacttgat gctcgatgag tttttctaat     1320

gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt     1380

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca agtcagaggt     1440

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     1500

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     1560

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     1620

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     1680

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     1740

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     1800

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     1860

cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     1920

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     1980

gattttctac cg                                                         1992


<210>  55
<211>  1896
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_T-TT7 plasmid

<400>  55
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgt agctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc      120

aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag      180

gaggaactat atccggatat cccgcaagag gcccggcagt acccctccga gacgactgac      240

catttaaatc atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt      300

gacttgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg      360

gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc      420

aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg      480

ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg      540

ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg      600

ttacagatga gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca      660

agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa      720

cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg      780

cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc      840

gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg      900

attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc      960

ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta     1020

tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc     1080

gataccagga tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga     1140

aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact     1200

tgatgctcga tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg     1260

tgggcctttc tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca     1320

aaaatcgatg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt     1380

ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc     1440

tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc     1500

tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc     1560

ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact     1620

tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg     1680

ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta     1740

tctgcgctct gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa     1800

acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa     1860

aaaaggatct caagaagatc ctttgatttt ctaccg                               1896


<210>  56
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES1 plasmid

<400>  56
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctctacctgg ctagagacgg caatacgcaa accgcctctc     1920

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctct     2940

gag                                                                   2943


<210>  57
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES2 plasmid

<400>  57
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctctacctgg ctagagacgg caatacgcaa accgcctctc     1920

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctca     2940

ggc                                                                   2943


<210>  58
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES3 plasmid

<400>  58
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctctaggcgg ctagagacgg caatacgcaa accgcctcta     1920

gccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctct     2940

gag                                                                   2943


<210>  59
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES4 plasmid

<400>  59
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctctaggcgg ctagagacgg caatacgcaa accgcctcta     1920

gccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctct     2940

gcc                                                                   2943


<210>  60
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES5 plasmid

<400>  60
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctcttgccgg ctagagacgg caatacgcaa accgcctcta     1920

gccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctct     2940

gag                                                                   2943


<210>  61
<211>  2943
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES6 plasmid

<400>  61
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctcttgccgg ctagagacgg caatacgcaa accgcctctc     1920

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagcg tctcacctcc     2940

act                                                                   2943


<210>  62
<211>  2942
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES7 plasmid

<400>  62
agagacccaa gacactgcgg ctttgtatgt gtccgcagcg cccgccgcag tctcacgccc       60

ggagcgtagc gaccgagtga gctagctatt tgtttatttt tctaaataca ttcaaatatg      120

tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt      180

atgagggaag cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc      240

gagcgccatc tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc      300

ggcctgaagc cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa      360

acaacgcggc gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc      420

gagattctcc gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt      480

tatccagcta agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt      540

atcttcgagc cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa      600

catagcgttg ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag      660

gatctatttg aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct      720

ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc      780

aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat      840

cagcccgtca tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc      900

tcgcgcgcag atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta      960

gtcggcaaat aatgtctaac aattcgttca agccgagggg ccgcaagatc cggccacgat     1020

gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt     1080

accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggtttc     1140

agcaaaaaac ccctcaagac ccgtttagag gccccaaggg gttatgctag ttattgctca     1200

gcggcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc     1260

gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat     1320

caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat     1380

actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct     1440

acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt     1500

cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg     1560

gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta     1620

cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg     1680

gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg     1740

tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc     1800

tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg     1860

gccgttggca gtgactcggt ctctcactgg ctagagacgg caatacgcaa accgcctcta     1920

gccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     1980

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     2040

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     2100

tactagagaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca     2160

tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg     2220

aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg     2280

gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt     2340

acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca     2400

aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct     2460

ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg     2520

acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc     2580

cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc     2640

actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg     2700

cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg     2760

aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct     2820

agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg     2880

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagacgt ctcacctctg     2940

ag                                                                    2942


<210>  63
<211>  4498
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCKH plasmid

<400>  63
actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc       60

cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta      120

tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg      180

gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc      240

cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca      300

tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat      360

agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa      420

agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtggtct      480

catgagcgag acgtccggca tccgcttaca gacaagctgt gacagtctcc gggagctgca      540

tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag actaaagggc ctcgtgatac      600

gcctattttt ataggttaat gtcatgataa taatggtttc ttaggacgga tcgcttgcct      660

gtaacttaca cgcgcctcgt atcttttaat gatggaataa tttgggaatt tactctgtgt      720

ttatttattt ttatgttttg tatttggatt ttagaaagta aataaagaag gtagaagagt      780

tacggaatga agaaaaaaaa ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt      840

tacatatata tttattagac aagaaaagca gattaaatag atatacattc gattaacgat      900

aagtaaaatg taaaatcaca ggattttcgt gtgtggtctt ctacacagac aagatgaaac      960

aattcggcat taatacctga gagcaggaag agcaagataa aaggtagtat ttgttggcga     1020

tccccctaga gtcttttaca tcttcggaaa acaaaaacta ttttttcttt aatttctttt     1080

tttactttct atttttaatt tatatattta tattaaaaaa tttaaattat aattattttt     1140

atagcacgtg atgaaaagga cccaggtggc attgacttga tcggcacgta agaggttcca     1200

actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt     1260

caggagctaa ggaagctaaa atgagccata ttcaacggga aacgtcttgc tcgaggccgc     1320

gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg     1380

ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc     1440

tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc aggctaaact     1500

ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg     1560

catggttact caccactgcg atcccaggga aaacagcatt ccaggtatta gaagaatatc     1620

ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga     1680

ttcctgtttg taattgtcct tttaacggcg atcgcgtatt tcgtctcgca caggcgcaat     1740

cacgaatgaa taacggtttg gttggtgcga gtgattttga tgacgagcgt aatggctggc     1800

ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg     1860

tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt     1920

gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga     1980

actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg     2040

ataatcctga tatgaataaa ttgcagtttc acttgatgct cgatgagttt ttctaatgag     2100

ggcccaaatg taatcacctg gctcaccttc gggtgggcct ttctgcgttg ctggcgtttt     2160

tccataggct ccgcccccct gacgagcatc acaaaaatcg atgctcaagt cagaggtggc     2220

gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct     2280

ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg     2340

tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca     2400

agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact     2460

atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta     2520

acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta     2580

actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct     2640

cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt     2700

ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat     2760

tttctaccga actgtgcggt atttcacacc gcatagatcc gtcgagttca agagaaaaaa     2820

aaagaaaaag caaaaagaaa aaaggaaagc gcgcctcgtt cagaatgaca cgtatagaat     2880

gatgcattac cttgtcatct tcagtatcat actgttcgta tacatactta ctgacattca     2940

taggtataca tatatacaca tgtatatata tcgtatgctg cagctttaaa taatcggtgt     3000

cactacataa gaacaccttt ggtggaggga acatcgttgg taccattggg cgaggtggct     3060

tctcttatgg caaccgcaag agccttgaac gcactctcac tacggtgatg atcattcttg     3120

cctcgcagac aatcaacgtg gagggtaatt ctgctagcct ctgcaaagct ttcaagaaaa     3180

tgcgggatca tctcgcaaga gagatctcct actttctccc tttgcaaacc aagttcgaca     3240

actgcgtacg gcctgttcga aagatctacc accgctctgg aaagtgcctc atccaaaggc     3300

gcaaatcctg atccaaacct ttttactcca cgcacggccc ctagggcctc tttaaaagct     3360

tgaccgagag caatcccgca gtcttcagtg gtgtgatggt cgtctatgtg taagtcacca     3420

atgcactcaa cgattagcga ccagccggaa tgcttggcca gagcatgtat catatggtcc     3480

agaaacccta tacctgtgtg gacgttaatc acttgcgatt gtgtggcctg ttctgctact     3540

gcttctgcct ctttttctgg gaagatcgag tgctctatcg ctaggggacc accctttaaa     3600

gagatcgcaa tctgaatctt ggtttcattt gtaatacgct ttactagggc tttctgctct     3660

gtcatctttg ccttcgttta tcttgcctgc tcatttttta gtatattctt cgaagaaatc     3720

acattacttt atataatgta taattcatta tgtgataatg ccaatcgcta agaaaaaaaa     3780

agagtcatcc gctaggtgga aaaaaaaaaa tgaaaatcat taccgaggca taaaaaaata     3840

tagagtgtac tagaggaggc caagagtaat agaaaaagaa aattgcggga aaggactgtg     3900

ttatgacttc cctgactaat gccgacgtct cgacctcgag accgcaatac gcaaaccgcc     3960

tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa     4020

agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc     4080

tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca     4140

cacatactag agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag     4200

ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa     4260

ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa     4320

ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa     4380

gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt     4440

ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccagg       4498


<210>  64
<211>  6033
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCKH-Cso-BMC plasmid

<400>  64
tgagcgagac gtccggcatc cgcttacaga caagctgtga cagtctccgg gagctgcatg       60

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac taaagggcct cgtgatacgc      120

ctatttttat aggttaatgt catgataata atggtttctt aggacggatc gcttgcctgt      180

aacttacacg cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt      240

atttattttt atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta      300

cggaatgaag aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta      360

catatatatt tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa      420

gtaaaatgta aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa      480

ttcggcatta atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc      540

cccctagagt cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt      600

tactttctat ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat      660

agcacgtgat gaaaaggacc caggtggcat tgacttgatc ggcacgtaag aggttccaac      720

tttcaccata atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca      780

ggagctaagg aagctaaaat gagccatatt caacgggaaa cgtcttgctc gaggccgcga      840

ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg      900

caatcaggtg cgacaatcta tcgattgtat gggaagcccg atgcgccaga gttgtttctg      960

aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag gctaaactgg     1020

ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca     1080

tggttactca ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct     1140

gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt     1200

cctgtttgta attgtccttt taacggcgat cgcgtatttc gtctcgcaca ggcgcaatca     1260

cgaatgaata acggtttggt tggtgcgagt gattttgatg acgagcgtaa tggctggcct     1320

gttgaacaag tctggaaaga aatgcataag cttttgccat tctcaccgga ttcagtcgtc     1380

actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt     1440

attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac     1500

tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat     1560

aatcctgata tgaataaatt gcagtttcac ttgatgctcg atgagttttt ctaatgaggg     1620

cccaaatgta atcacctggc tcaccttcgg gtgggccttt ctgcgttgct ggcgtttttc     1680

cataggctcc gcccccctga cgagcatcac aaaaatcgat gctcaagtca gaggtggcga     1740

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     1800

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     1860

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     1920

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     1980

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     2040

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     2100

tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttacctcg     2160

gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt     2220

ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgattt     2280

tctaccgaac tgtgcggtat ttcacaccgc atagatccgt cgagttcaag agaaaaaaaa     2340

agaaaaagca aaaagaaaaa aggaaagcgc gcctcgttca gaatgacacg tatagaatga     2400

tgcattacct tgtcatcttc agtatcatac tgttcgtata catacttact gacattcata     2460

ggtatacata tatacacatg tatatatatc gtatgctgca gctttaaata atcggtgtca     2520

ctacataaga acacctttgg tggagggaac atcgttggta ccattgggcg aggtggcttc     2580

tcttatggca accgcaagag ccttgaacgc actctcacta cggtgatgat cattcttgcc     2640

tcgcagacaa tcaacgtgga gggtaattct gctagcctct gcaaagcttt caagaaaatg     2700

cgggatcatc tcgcaagaga gatctcctac tttctccctt tgcaaaccaa gttcgacaac     2760

tgcgtacggc ctgttcgaaa gatctaccac cgctctggaa agtgcctcat ccaaaggcgc     2820

aaatcctgat ccaaaccttt ttactccacg cacggcccct agggcctctt taaaagcttg     2880

accgagagca atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat     2940

gcactcaacg attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag     3000

aaaccctata cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc     3060

ttctgcctct ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga     3120

gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt     3180

catctttgcc ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac     3240

attactttat ataatgtata attcattatg tgataatgcc aatcgctaag aaaaaaaaag     3300

agtcatccgc taggtggaaa aaaaaaaatg aaaatcatta ccgaggcata aaaaaatata     3360

gagtgtacta gaggaggcca agagtaatag aaaaagaaaa ttgcgggaaa ggactgtgtt     3420

atgacttccc tgactaatgc cgacgtctcg acctggctgg cttcccaacc ttaccagagg     3480

gcgccccagc tggcaattcc gacgtcttta tggctagctc agtcctaggt acaatgctag     3540

cgaattcaaa agatctttta agaaggagat atacatgatg aagatcatgc aagttgaaaa     3600

gactttggtt tctaccaaca gaattgctga tatgggtcac aagccattgt tggttgtttg     3660

ggaaaaacct ggtgctccaa gacaagttgc tgttgatgct attggttgta ttccaggtga     3720

ctgggttttg tgtgttggtt cttctgctgc cagagaagct gctggttcca agtcttaccc     3780

atctgatttg actatcatcg gtattattga ccaatggaac ggtgaaggtt cttcttggtc     3840

acatccacaa tttgaaaagt agctaacaaa gcccgaaagg aagctgagtt ggctgctgcc     3900

accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt     3960

ttgctgaaag gaggaactat atccggatat cccgcaagag gcccggcagt acccctcagg     4020

cggcttcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg     4080

gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc     4140

agtgaaacgg gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag     4200

cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg     4260

atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg     4320

cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc     4380

agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac     4440

atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat     4500

ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc     4560

gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca     4620

tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga     4680

acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg     4740

atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg     4800

ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta     4860

atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc     4920

agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc     4980

gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc     5040

acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact     5100

ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga     5160

aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg     5220

cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg     5280

tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac     5340

gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc     5400

ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc     5460

ggcgtagagg atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg     5520

tgagcggata acaattcccc tctagaaata attttgttta actttaagaa ggagatatac     5580

gatggctgat gttactggta ttgctttggg tatgattgaa actagaggtt tggttccagc     5640

tatcgaagct gctgacgcta tgaccaaggc cgctgaagtc agattggtcg gtagacaatt     5700

tgttggaggt ggttacgtca ctgttttggt tcgtggtgaa accggtgccg ttaacgctgc     5760

tgttagagct ggtgctgatg cttgtgaaag agttggtgac ggtttagttg ctgcccacat     5820

tattgccaga gtccactctg aagttgaaaa cattttgcca aaggctccac aggcttagct     5880

aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa     5940

ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc     6000

ggatatcccg caagaggccc ggcagtaccc ctc                                  6033


<210>  65
<211>  2246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-TDH3 plasmid

<400>  65
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctacagttt attcctggca tccactaaat ataatggagc ccgcttttta      120

agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct tcaccaacca      180

tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca aacaggcaaa      240

aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat gacacaaggc      300

aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct tctgctctct      360

ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt attcccctac      420

ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa tctatttctt      480

aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa caccaagaac      540

ttagtttcga ataaacacac ataaacaaac aaagatgcga gacgactgac catttaaatc      600

atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg      660

gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt      720

tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac      780

gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg      840

ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga      900

tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga      960

gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat     1020

ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca     1080

ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct     1140

gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg     1200

tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga     1260

cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt     1320

ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga     1380

ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga     1440

tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt     1500

tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga     1560

tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc     1620

tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg     1680

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     1740

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     1800

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     1860

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     1920

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     1980

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     2040

cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     2100

gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     2160

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     2220

caagaagatc ctttgatttt ctaccg                                          2246


<210>  66
<211>  2246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-YEF3 plasmid

<400>  66
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctattaaaa aaacaactta caatcattgt tcgccccttc catacttact      120

gccactcgca aaagggccca accagggcaa ttacgtatca aaaaatcatg acaggctggg      180

taataaatat tcgtgaagaa agaagaaatt aaaaaaagaa acgaagaagc aaaaaaaaga      240

aaagactccg tttaatcact ttcaaccgcg gtttatccgg ccccacccat gcataaccct      300

aaattattag atcacttagc acgtgaaaaa gaaacgtttt taatgttttt tttttttttt      360

tctttttctt tttttgcgtt ggtgaaaatt ttttcgcttc ctcgagtata attatctcat      420

ctcatctttc atataagata agaagtttta taaaaacctt ttgcatcaaa attttgtaga      480

atatctcttt ttcttacgct ctctttcttt ccttaattgt tttctaaaga accgtgtatt      540

tttctagttc gaatccatcg ataacattaa aaggatgcga gacgactgac catttaaatc      600

atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg      660

gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt      720

tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac      780

gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg      840

ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga      900

tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga      960

gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat     1020

ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca     1080

ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct     1140

gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg     1200

tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga     1260

cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt     1320

ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga     1380

ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga     1440

tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt     1500

tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga     1560

tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc     1620

tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg     1680

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     1740

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     1800

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     1860

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     1920

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     1980

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     2040

cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     2100

gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     2160

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     2220

caagaagatc ctttgatttt ctaccg                                          2246


<210>  67
<211>  2246
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-PYK1 plasmid

<400>  67
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctacagatt gggagatttt catagtagaa ttcagcatga tagctacgta      120

aatgtgttcc gcaccgtcac aaagtgtttt ctactgttct ttcttctttc gttcattcag      180

ttgagttgag tgagtgcttt gttcaatgga tcttagctaa aatgcatatt ttttctcttg      240

gtaaatgaat gcttgtgatg tcttccaagt gatttccttt ccttcccata tgatgctagg      300

tacctttagt gtcttcctaa aaaaaaaaaa aggctcgcca tcaaaacgat attcgttggc      360

ttttttttct gaattataaa tactctttgg taacttttca tttccaagaa cctctttttt      420

ccagttatat catggtcccc tttcaaagtt attctctact ctttttcata ttcattcttt      480

ttcatccttt ggttttttat tcttaacttg tttattattc tctcttgttt ctatttacaa      540

gacaccaatc aaaacaaata aaacatcatc acagatgcga gacgactgac catttaaatc      600

atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg      660

gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt      720

tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac      780

gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg      840

ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga      900

tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga      960

gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat     1020

ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca     1080

ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct     1140

gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg     1200

tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga     1260

cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt     1320

ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga     1380

ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga     1440

tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt     1500

tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga     1560

tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc     1620

tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg     1680

ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg     1740

aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt     1800

tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt     1860

gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg     1920

cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact     1980

ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt     2040

cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct     2100

gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc     2160

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct     2220

caagaagatc ctttgatttt ctaccg                                          2246


<210>  68
<211>  2040
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-H plasmid

<400>  68
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atggctgatg ctttgggtat gattgaagtt agaggtttcg ttggtatggt      120

tgaagctgct gatgctatgg ttaaggctgc taaagttgaa ttgatcggtt acgaaaaaac      180

tggtggtggt tatgttactg ctgttgttag aggtgatgtt gctgctgtaa aagctgctac      240

tgaagctggt caaagggctg ctgaaagagt tggagaagtt gttgctgttc atgttattcc      300

aagaccacat gttaatgttg atgctgcttt gccattgggt agaactccag gtatggataa      360

gtctgcttag ccgagacgac tgaccattta aatcatacct gacctccata gcagaaagtc      420

aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc aactttcacc      480

ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta      540

aggaagctaa aatgagccat attcaacggg aaacgtcttg ctcgaggccg cgattaaatt      600

ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc gggcaatcag      660

gtgcgacaat ctatcgattg tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg      720

gcaaaggtag cgttgccaat gatgttacag atgagatggt caggctaaac tggctgacgg      780

aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat gcatggttac      840

tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat cctgattcag      900

gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg attcctgttt      960

gtaattgtcc ttttaacggc gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga     1020

ataacggttt ggttggtgcg agtgattttg atgacgagcg taatggctgg cctgttgaac     1080

aagtctggaa agaaatgcat aagcttttgc cattctcacc ggattcagtc gtcactcatg     1140

gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt tgtattgatg     1200

ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg aactgcctcg     1260

gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt gataatcctg     1320

atatgaataa attgcagttt cacttgatgc tcgatgagtt tttctaatga gggcccaaat     1380

gtaatcacct ggctcacctt cgggtgggcc tttctgcgtt gctggcgttt ttccataggc     1440

tccgcccccc tgacgagcat cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga     1500

caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc     1560

cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt     1620

ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct     1680

gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg     1740

agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta     1800

gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct     1860

acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag     1920

agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg     1980

caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga ttttctaccg     2040


<210>  69
<211>  2031
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-P plasmid

<400>  69
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atggttttag gtaaagttgt cggtactgtt gttgcatcaa gaaaggaacc      120

aagaattgaa ggtttatctt tattattggt tagagcttgt gatccagatg gtactccaac      180

tggtggtgct gttgtttgtg ctgatgctgt tggtgctggt gttggtgaag ttgttttata      240

tgcttctggt tcttctgcta gacaaactga agttactaat aatagaccag ttgatgctac      300

tattatggct attgttgatt tggttgaaat gggtggtgat gttagattta gaaaagatta      360

gccgagacga ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc      420

cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa      480

taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta      540

aaatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg      600

atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa      660

tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta      720

gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc      780

ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg      840

cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata      900

ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc      960

cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt     1020

tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga     1080

aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct     1140

cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag     1200

tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt     1260

ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata     1320

aattgcagtt tcacttgatg ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc     1380

tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc     1440

ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat     1500

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     1560

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     1620

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     1680

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     1740

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     1800

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     1860

gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag     1920

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     1980

gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc g              2031


<210>  70
<211>  2358
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-T1 plasmid

<400>  70
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atggatcatg ctccagaaag atttgatgct actcctccag ctggtgaacc      120

agatagacca gctttgggtg ttttggaatt gacttctatt gctagaggta ttaccgttgc      180

tgatgctgct ttgaaaagag caccatcttt gttgttgatg tccagaccag tttcttccgg      240

taaacatttg ttgatgatga gaggtcaagt tgccgaagtt gaagaatcta tgattgctgc      300

tagagaaatt gctggtgctg gttctggtgc tttgttggat gaattggaat tgccatatgc      360

tcacgaacaa ctttggagat ttttggatgc tccagttgtt gcagatgctt gggaagaaga      420

tactgaatcc gttattatcg ttgaaaccgc tactgtttgt gctgctattg attctgctga      480

tgcagcctta aaaactgctc ctgttgtttt gagagatatg agattggcta ttggtattgc      540

tggtaaggct ttctttactt tgactggtga attggctgat gttgaagctg ctgctgaagt      600

tgttagagaa agatgtggtg ctagattgct agaattggct tgtattgcaa gaccagttga      660

cgaattgaga ggtaggttgt ttttctagcc gagacgactg accatttaaa tcatacctga      720

cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa      780

gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat      840

cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct      900

cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg      960

ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag     1020

agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca     1080

ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc     1140

ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag     1200

aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt     1260

tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc     1320

aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta     1380

atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg     1440

attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat     1500

taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca     1560

tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat     1620

atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt     1680

tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc     1740

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc     1800

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     1860

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     1920

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     1980

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     2040

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     2100

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     2160

ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc     2220

cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     2280

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     2340

tcctttgatt ttctaccg                                                   2358


<210>  71
<211>  2201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_T-RPL41B plasmid

<400>  71
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgt agcgcggatt gagagcaaat cgttaagttc aggtcaagta aaaattgatt      120

tcgaaaacta atttctctta tacaatcctt tgattggacc gtcatccttt cgaatataag      180

attttgttaa gaatatttta gacagagatc tactttatat ttaatatcta gatattacat      240

aatttcctct ctaataaaat atcattaata aaataaaaat gaagcgattt gattttgtgt      300

tgtcaactta gtttgccgct atgcctcttg ggtaatgcta ttattgaatc gaagggcttt      360

attatattac cctttagctt attctgaggt ttctgtggcg tgcaaagtga tgaaccgggc      420

gggttttaag gataaaatca aaaagtgaaa aaatgaacgg aaaatggaat acctgtgaaa      480

tggagaatga taatgaatct ttctgtcgtg cttgaaagat tttcggctcc tccgagacga      540

ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg      600

cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact      660

accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca      720

tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt      780

atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt      840

gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa      900

tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac      960

catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg     1020

gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc     1080

gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg     1140

cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc     1200

gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca     1260

taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa     1320

ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc     1380

agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt     1440

acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt     1500

tcacttgatg ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc tggctcacct     1560

tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca     1620

tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca     1680

ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg     1740

atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag     1800

gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt     1860

tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca     1920

cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg     1980

cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt     2040

tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc     2100

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc     2160

agaaaaaaag gatctcaaga agatcctttg attttctacc g                         2201


<210>  72
<211>  2172
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_T-HBT1 plasmid

<400>  72
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgt agcacacttc tcgattaaca aattcccagt attctttgaa atctattttt      120

cttcctcaat tgaatttgaa taactgtcta cgcggactcc tcctatctac aactacaaca      180

aattttaacc actttattac cactttcctc tttcatttat ttttgtcttt tatgttgtca      240

atttactagt attttttttt ttttcattta cgttcaaggt tttttatact catttaactt      300

gtcttaggtt atttatatat atacctatat atttatatat atatatatat atgtatgtat      360

atattattat caccaaatga gaaataatag ctaatttgat ttttgattat ttaaaatatt      420

ggtttgttct ttctgcaaac atctcgtttg gtacgatatt agtgaaaaac gatgtaatta      480

tcaacacgtg cattacccac ctccgagacg actgaccatt taaatcatac ctgacctcca      540

tagcagaaag tcaaaagcct ccgaccggag gcttttgact tgatcggcac gtaagaggtt      600

ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat      660

tttcaggagc taaggaagct aaaatgagcc atattcaacg ggaaacgtct tgctcgaggc      720

cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg      780

tcgggcaatc aggtgcgaca atctatcgat tgtatgggaa gcccgatgcg ccagagttgt      840

ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcaggctaa      900

actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg      960

atgcatggtt actcaccact gcgatcccag ggaaaacagc attccaggta ttagaagaat     1020

atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt     1080

cgattcctgt ttgtaattgt ccttttaacg gcgatcgcgt atttcgtctc gctcaggcgc     1140

aatcacgaat gaataacggt ttggttggtg cgagtgattt tgatgacgag cgtaatggct     1200

ggcctgttga acaagtctgg aaagaaatgc ataagctttt gccattctca ccggattcag     1260

tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag     1320

gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat     1380

ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta     1440

ttgataatcc tgatatgaat aaattgcagt ttcacttgat gctcgatgag tttttctaat     1500

gagggcccaa atgtaatcac ctggctcacc ttcgggtggg cctttctgcg ttgctggcgt     1560

ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgatgctca agtcagaggt     1620

ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc     1680

gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa     1740

gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct     1800

ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta     1860

actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg     1920

gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc     1980

ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta     2040

cctcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg     2100

tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt     2160

gattttctac cg                                                         2172


<210>  73
<211>  2200
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_T-RPS20 plasmid

<400>  73
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgt agcaactaag ctggttctaa ctggaaataa tttccattag attcctcttt      120

ttctcgtcca ttaaccaaaa tatattattg aattcagcgg ttcctttttt ctcattttcg      180

catatagctg cactattaga atcagcccac tctaggtaaa cacagttcct cgatatacct      240

ctgtcttact atcagtggtt aaaccttatg caaatataat atatatatat atatatatat      300

ctcatacttt tgttgattct tgtgtaatta ttggaaaaga caaaacaaag caagcgtttc      360

tattcatcat atttacaagt atttttatga aaaactattt cttaattttc ccaccggcgg      420

ctttgaataa ggcaatgtca ttgtcctgca taatatattg tttgcctgca cgtttgataa      480

gtcccttaga ttttagtaaa gactcattta gcggtggttc catcttccct ccgagacgac      540

tgaccattta aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc      600

ttttgacttg atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta      660

ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat      720

attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta      780

tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg      840

tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat      900

gatgttacag atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc      960

atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg     1020

aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg     1080

ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc     1140

gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttggtgcg     1200

agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat     1260

aagcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac     1320

cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca     1380

gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta     1440

cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt     1500

cacttgatgc tcgatgagtt tttctaatga gggcccaaat gtaatcacct ggctcacctt     1560

cgggtgggcc tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat     1620

cacaaaaatc gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag     1680

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga     1740

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg     1800

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt     1860

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac     1920

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc     1980

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt     2040

ggtatctgcg ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg     2100

gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca     2160

gaaaaaaagg atctcaagaa gatcctttga ttttctaccg                           2200


<210>  74
<211>  4531
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCKU plasmid

<400>  74
actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc       60

cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta      120

tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg      180

gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc      240

cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca      300

tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat      360

agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa      420

agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtggtct      480

catgagcgag acgtccggca tccgcttaca gacaagctgt gacaatctcc gggagctgca      540

tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag attaaagggc ctcgtgatac      600

gcctattttt ataggttaat gtcatgataa taatggtttc ttagacggat cgcttgcctg      660

taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt      720

tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt      780

acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt      840

acatatatat ttattagaca agaaaagcag attaaataga tatacattcg attaacgata      900

agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca      960

attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat     1020

ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt     1080

ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata attattttta     1140

tagcacgtga tgaaaaggac ccaggtggca ttgacttgat cggcacgtaa gaggttccaa     1200

ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc     1260

aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct cgaggccgcg     1320

attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg     1380

gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag agttgtttct     1440

gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca ggctaaactg     1500

gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc     1560

atggttactc accactgcga tcccagggaa aacagcattc caggtattag aagaatatcc     1620

tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat     1680

tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgcac aggcgcaatc     1740

acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta atggctggcc     1800

tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg attcagtcgt     1860

cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg     1920

tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa     1980

ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga     2040

taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt tctaatgagg     2100

gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc tggcgttttt     2160

ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc agaggtggcg     2220

aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc     2280

tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt     2340

ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa     2400

gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta     2460

tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa     2520

caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa     2580

ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctc     2640

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     2700

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatt     2760

ttctaccgaa cgtttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta     2820

tttcacaccg catagggtaa taactgatat aattaaattg aagctctaat ttgtgagttt     2880

agtatacatg catttactta taatacagtt ttttagtttt gctggccgca tcttctcaaa     2940

tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc ccttcccttt     3000

gcaaatagtc ctcttccaac aataataatg tcagatcctg tagacaccac atcatccacg     3060

gttctatact gttgacccaa tgcgtcaccc ttgtcatcta aacccacacc gggtgtcata     3120

atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat aaagccgata     3180

acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc tccagtagat     3240

agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc ctttgttact     3300

tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc gtgtgcattc     3360

gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa tttgactgta     3420

ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt ggcggataat     3480

gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc cacatgtgtt     3540

tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc cttggtggta     3600

cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt aaatagcttg     3660

gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt cgacatgatt     3720

tatcttcgtt tcctgcaggt ttttgttctg tgcagttggg ttaagaatac tgggcaattt     3780

catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg ctccttcctt     3840

cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa ccgaaatcaa     3900

aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag ctgtggtatg gtgcactacg     3960

tctcgacctc gagaccgcaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta     4020

atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa     4080

tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat     4140

gttgtgtgga attgtgagcg gataacaatt tcacacatac tagagaaaga ggagaaatac     4200

tagatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa     4260

ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa     4320

ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac     4380

atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc     4440

ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc     4500

gaagacggtg gtgttgttac cgttacccag g                                    4531


<210>  75
<211>  6441
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGAU-YMRW?15 plasmid

<400>  75
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc       60

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact      120

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct      180

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag      240

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca      300

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa      360

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc      420

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag      480

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg      540

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca      600

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc      660

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag      720

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata      780

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat      840

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg      900

ggagggctta ccatctggcc ccagtgctgc aatgataccg cggctcccac gctcaccggc      960

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     1020

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     1080

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     1140

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     1200

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     1260

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     1320

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     1380

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     1440

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     1500

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     1560

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     1620

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     1680

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     1740

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ggtctctgtc     1800

atcaatcaaa gcaacccaca aatcctaggc tgaatcatga tatcgatgga agcaatcaac     1860

aattttatca agaccgcacc aaagcacgac tatctgacag gcggagttca tcattctggt     1920

aatgtagacg tgttacaatt aagcggcaat aaagaagatg gtagtttagt atggaaccat     1980

acttttgttg atgtagacaa caatgtggta gctaagtttg aagacgctct cgaaaaactt     2040

gaaagtttgc accggcgctc atcctcatcc acaggcaatg aagaacacgc taacgtttaa     2100

ccgaggggag tcacttcata atgatgtgag aaataagtga atattgtaat aattgttggg     2160

actccattgt caacaaaagc tataatgtag gtatacagta tatactagaa gttctcctcg     2220

aggatcttgg aatccacaaa agggagtcga taaatctata taataaaaat tactttatct     2280

tctttcgttt tatacgttgt cgtttattat cctattacgt tatcaatctt cgcatttcag     2340

ctttcattag atttgatgac tgtttctcaa actttatgtc attttcttac accgctctct     2400

acctggctcg aagcacgcta gtaacatcag ctaacgaaag agttagaggc tcgctaaatc     2460

gcactgtcgg ggtcccttgg gtattttaca ctagcgtcag gacgactagc atgtgtcttt     2520

ccttccaggg gtatgcgggt gcgtggacaa atgagcagca tacgtattta ctcggcgtgc     2580

ctgctctctc gtatttctcc tggagatcaa ggaaatgttt catgtccaag cgaaaagccg     2640

ctctacggaa tggatctacg ttactgcctg cataaggaaa ccggtgtagc caaggacgaa     2700

agcgacccta ggttctaacc atcgactttg gcggaaaggt ttcactcagg aagcagacac     2760

tgattgacac ggtttagcag aacgtttgag gactaggtca aattgagtgg tttaatatcg     2820

gcatgtctgg ctttaaaatt cagtatagtg cgctgatcgg aaacgaatta aaaacacgag     2880

ttcccaaaac caggcgggct cgccacgcta atcgggatgc ataccacagc ttttcaattc     2940

aattcatcat ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat     3000

tcggtaatct ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat     3060

atacgcatat gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac     3120

agaacaaaaa cctgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt     3180

gctgctactc atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa     3240

acaaacttgt gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa     3300

gcattaggtc ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc     3360

atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc     3420

gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta     3480

tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt     3540

gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg     3600

ttagcagaat tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt     3660

gacattgcga agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt     3720

ggaagagatg aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag     3780

ggtgacgcat tgggtcaaca gtatagaacc gtggatgatg tggtgtctac aggatctgac     3840

attattattg ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa     3900

cgttacagaa aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa     3960

actgtattat aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta     4020

tatcagttat taccctatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg     4080

catcaggtag ccgctagtaa catcagctaa cgaaagagtt agaggctcgc taaatcgcac     4140

tgtcggggtc ccttgggtat tttacactag cgtcaggacg actagcatgt gtctttcctt     4200

ccaggggtat gcgggtgcgt ggacaaatga gcagcatacg tatttactcg gcgtgcctgc     4260

tctctcgtat ttctcctgga gatcaaggaa atgtttcatg tccaagcgaa aagccgctct     4320

acggaatgga tctacgttac tgcctgcata aggaaaccgg tgtagccaag gacgaaagcg     4380

accctaggtt ctaaccatcg actttggcgg aaaggtttca ctcaggaagc agacactgat     4440

tgacacggtt tagcagaacg tttgaggact aggtcaaatt gagtggttta atatcggcat     4500

gtctggcttt aaaattcagt atagtgcgct gatcggaaac gaattaaaaa cacgagttcc     4560

caaaaccagg cgggctcgcc acgctaatcg gtgcaccacc tcaggcagag aacctagaga     4620

cggcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg     4680

acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca     4740

ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg     4800

tgagcggata acaatttcac acatactaga gaaagaggag aaatactaga tggcttcctc     4860

cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt atggaaggtt ccgttaacgg     4920

tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta cccagaccgc     4980

taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc tgtccccgca     5040

gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg actacctgaa     5100

actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag acggtggtgt     5160

tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca aagttaaact     5220

gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca tgggttggga     5280

agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa tcaaaatgcg     5340

tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct acatggctaa     5400

aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca tcacctccca     5460

caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc actccaccgg     5520

tgcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa     5580

cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct     5640

ctctactaga gtcacactgg ctccgtctca tgagcgctca tggaaaatgc aaccgataaa     5700

ccattataaa tcttcgcggt tatctggcat tgttattaac caaaaaaatg ccggcctatt     5760

acaagctact gttcaataaa tattgttgta atgaagacgg tccaactgta caaatacagc     5820

aaactgtcat atataaggag tcttatgtga cagcacttgc gttattgtca gccggagtat     5880

gtctttgtcg cattctgggc tttttacttt ctgctcagaa ggaagtacga acaagaaaaa     5940

aaaatcacca atgcttccct tttcagtatt agtttcatat ttgtttacgt tcaaactcgt     6000

cgtttgcgcg ataacctcta aaaaagtcaa ttacgtaact atatcaatca gagaatgcaa     6060

aaagcactat cataaaaatg tgtctagggg atgtgagaca tgtcaattat aagaagtgat     6120

ggtgtcatag tatatatatc ataaaagatt atcaaagttt caatcctttg tattttctag     6180

tttagcgcca acttttgaca aaacctaaac tttagataat catcattctt acaattttta     6240

tctggatggc aataatctcc tatataaagc ccagataaac tgtaaaaaga atccatcact     6300

atttgaaaaa aagtcatctg gcacgtttaa ttatcagagc agaaatgatg aagggtgtta     6360

gcgccgtcca ctgatgtgcc tggtagtcat gatttacgta taactaacac atcatgagga     6420

cggcggctcg gagagaccga t                                               6441


<210>  76
<211>  7606
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGAU-YMRW?15-HO-BMC plasmid

<400>  76
tgagcgagac gtccggcatc cgcttacaga caagctgtga caatctccgg gagctgcatg       60

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagat taaagggcct cgtgatacgc      120

ctatttttat aggttaatgt catgataata atggtttctt agacggatcg cttgcctgta      180

acttacacgc gcctcgtatc ttttaatgat ggaataattt gggaatttac tctgtgttta      240

tttattttta tgttttgtat ttggatttta gaaagtaaat aaagaaggta gaagagttac      300

ggaatgaaga aaaaaaaata aacaaaggtt taaaaaattt caacaaaaag cgtactttac      360

atatatattt attagacaag aaaagcagat taaatagata tacattcgat taacgataag      420

taaaatgtaa aatcacagga ttttcgtgtg tggtcttcta cacagacaag atgaaacaat      480

tcggcattaa tacctgagag caggaagagc aagataaaag gtagtatttg ttggcgatcc      540

ccctagagtc ttttacatct tcggaaaaca aaaactattt tttctttaat ttcttttttt      600

actttctatt tttaatttat atatttatat taaaaaattt aaattataat tatttttata      660

gcacgtgatg aaaaggaccc aggtggcatt gacttgatcg gcacgtaaga ggttccaact      720

ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag      780

gagctaagga agctaaaatg agccatattc aacgggaaac gtcttgctcg aggccgcgat      840

taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc      900

aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga      960

aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcagg ctaaactggc     1020

tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat     1080

ggttactcac cactgcgatc ccagggaaaa cagcattcca ggtattagaa gaatatcctg     1140

attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc     1200

ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg tctcgcacag gcgcaatcac     1260

gaatgaataa cggtttggtt ggtgcgagtg attttgatga cgagcgtaat ggctggcctg     1320

ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca     1380

ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta     1440

ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact     1500

gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata     1560

atcctgatat gaataaattg cagtttcact tgatgctcga tgagtttttc taatgagggc     1620

ccaaatgtaa tcacctggct caccttcggg tgggcctttc tgcgttgctg gcgtttttcc     1680

ataggctccg cccccctgac gagcatcaca aaaatcgatg ctcaagtcag aggtggcgaa     1740

acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc     1800

ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg     1860

cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc     1920

tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc     1980

gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca     2040

ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact     2100

acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttacctcgg     2160

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     2220

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatttt     2280

ctaccgaacg tttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt     2340

tcacaccgca tagggtaata actgatataa ttaaattgaa gctctaattt gtgagtttag     2400

tatacatgca tttacttata atacagtttt ttagttttgc tggccgcatc ttctcaaata     2460

tgcttcccag cctgcttttc tgtaacgttc accctctacc ttagcatccc ttccctttgc     2520

aaatagtcct cttccaacaa taataatgtc agatcctgta gacaccacat catccacggt     2580

tctatactgt tgacccaatg cgtcaccctt gtcatctaaa cccacaccgg gtgtcataat     2640

caaccaatcg taaccttcat ctcttccacc catgtctctt tgagcaataa agccgataac     2700

aaaatctttg tcgctcttcg caatgtcaac agtaccctta gtatattctc cagtagatag     2760

ggagcccttg catgacaatt ctgctaacat caaaaggcct ctaggttcct ttgttacttc     2820

ttctgccgcc tgcttcaaac cgctaacaat acctgggccc accacaccgt gtgcattcgt     2880

aatgtctgcc cattctgcta ttctgtatac acccgcagag tactgcaatt tgactgtatt     2940

accaatgtca gcaaattttc tgtcttcgaa gagtaaaaaa ttgtacttgg cggataatgc     3000

ctttagcggc ttaactgtgc cctccatgga aaaatcagtc aagatatcca catgtgtttt     3060

tagtaaacaa attttgggac ctaatgcttc aactaactcc agtaattcct tggtggtacg     3120

aacatccaat gaagcacaca agtttgtttg cttttcgtgc atgatattaa atagcttggc     3180

agcaacagga ctaggatgag tagcagcacg ttccttatat gtagctttcg acatgattta     3240

tcttcgtttc ctgcaggttt ttgttctgtg cagttgggtt aagaatactg ggcaatttca     3300

tgtttcttca acactacata tgcgtatata taccaatcta agtctgtgct ccttccttcg     3360

ttcttccttc tgttcggaga ttaccgaatc aaaaaaattt caaagaaacc gaaatcaaaa     3420

aaaagaataa aaaaaaaatg atgaattgaa ttgaaaagct gtggtatggt gcactacgtc     3480

tcgacctggc tacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag     3540

ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc     3600

agttcatagg tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa     3660

acgggcacaa cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa     3720

ttgacccacg catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct     3780

gatttggaaa aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt     3840

gactaataag tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa     3900

acttcttaaa ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt     3960

agtttcgaat aaacacacat aaacaaacaa agatggctga tgctttgggt atgattgaag     4020

ttagaggttt cgttggtatg gttgaagctg ctgatgctat ggttaaggct gctaaagttg     4080

aattgatcgg ttacgaaaaa actggtggtg gttatgttac tgctgttgtt agaggtgatg     4140

ttgctgctgt aaaagctgct actgaagctg gtcaaagggc tgctgaaaga gttggagaag     4200

ttgttgctgt tcatgttatt ccaagaccac atgttaatgt tgatgctgct ttgccattgg     4260

gtagaactcc aggtatggat aagtctgctt agcgcggatt gagagcaaat cgttaagttc     4320

aggtcaagta aaaattgatt tcgaaaacta atttctctta tacaatcctt tgattggacc     4380

gtcatccttt cgaatataag attttgttaa gaatatttta gacagagatc tactttatat     4440

ttaatatcta gatattacat aatttcctct ctaataaaat atcattaata aaataaaaat     4500

gaagcgattt gattttgtgt tgtcaactta gtttgccgct atgcctcttg ggtaatgcta     4560

ttattgaatc gaagggcttt attatattac cctttagctt attctgaggt ttctgtggcg     4620

tgcaaagtga tgaaccgggc gggttttaag gataaaatca aaaagtgaaa aaatgaacgg     4680

aaaatggaat acctgtgaaa tggagaatga taatgaatct ttctgtcgtg cttgaaagat     4740

tttcggctcc tcaggcggct attaaaaaaa caacttacaa tcattgttcg ccccttccat     4800

acttactgcc actcgcaaaa gggcccaacc agggcaatta cgtatcaaaa aatcatgaca     4860

ggctgggtaa taaatattcg tgaagaaaga agaaattaaa aaaagaaacg aagaagcaaa     4920

aaaaagaaaa gactccgttt aatcactttc aaccgcggtt tatccggccc cacccatgca     4980

taaccctaaa ttattagatc acttagcacg tgaaaaagaa acgtttttaa tgtttttttt     5040

ttttttttct ttttcttttt ttgcgttggt gaaaattttt tcgcttcctc gagtataatt     5100

atctcatctc atctttcata taagataaga agttttataa aaaccttttg catcaaaatt     5160

ttgtagaata tctctttttc ttacgctctc tttctttcct taattgtttt ctaaagaacc     5220

gtgtattttt ctagttcgaa tccatcgata acattaaaag gatggatcat gctccagaaa     5280

gatttgatgc tactcctcca gctggtgaac cagatagacc agctttgggt gttttggaat     5340

tgacttctat tgctagaggt attaccgttg ctgatgctgc tttgaaaaga gcaccatctt     5400

tgttgttgat gtccagacca gtttcttccg gtaaacattt gttgatgatg agaggtcaag     5460

ttgccgaagt tgaagaatct atgattgctg ctagagaaat tgctggtgct ggttctggtg     5520

ctttgttgga tgaattggaa ttgccatatg ctcacgaaca actttggaga tttttggatg     5580

ctccagttgt tgcagatgct tgggaagaag atactgaatc cgttattatc gttgaaaccg     5640

ctactgtttg tgctgctatt gattctgctg atgcagcctt aaaaactgct cctgttgttt     5700

tgagagatat gagattggct attggtattg ctggtaaggc tttctttact ttgactggtg     5760

aattggctga tgttgaagct gctgctgaag ttgttagaga aagatgtggt gctagattgc     5820

tagaattggc ttgtattgca agaccagttg acgaattgag aggtaggttg tttttctagc     5880

acacttctcg attaacaaat tcccagtatt ctttgaaatc tatttttctt cctcaattga     5940

atttgaataa ctgtctacgc ggactcctcc tatctacaac tacaacaaat tttaaccact     6000

ttattaccac tttcctcttt catttatttt tgtcttttat gttgtcaatt tactagtatt     6060

tttttttttt tcatttacgt tcaaggtttt ttatactcat ttaacttgtc ttaggttatt     6120

tatatatata cctatatatt tatatatata tatatatatg tatgtatata ttattatcac     6180

caaatgagaa ataatagcta atttgatttt tgattattta aaatattggt ttgttctttc     6240

tgcaaacatc tcgtttggta cgatattagt gaaaaacgat gtaattatca acacgtgcat     6300

tacccacctc tgccggctac agattgggag attttcatag tagaattcag catgatagct     6360

acgtaaatgt gttccgcacc gtcacaaagt gttttctact gttctttctt ctttcgttca     6420

ttcagttgag ttgagtgagt gctttgttca atggatctta gctaaaatgc atattttttc     6480

tcttggtaaa tgaatgcttg tgatgtcttc caagtgattt cctttccttc ccatatgatg     6540

ctaggtacct ttagtgtctt cctaaaaaaa aaaaaaggct cgccatcaaa acgatattcg     6600

ttggcttttt tttctgaatt ataaatactc tttggtaact tttcatttcc aagaacctct     6660

tttttccagt tatatcatgg tcccctttca aagttattct ctactctttt tcatattcat     6720

tctttttcat cctttggttt tttattctta acttgtttat tattctctct tgtttctatt     6780

tacaagacac caatcaaaac aaataaaaca tcatcacaga tggttttagg taaagttgtc     6840

ggtactgttg ttgcatcaag aaaggaacca agaattgaag gtttatcttt attattggtt     6900

agagcttgtg atccagatgg tactccaact ggtggtgctg ttgtttgtgc tgatgctgtt     6960

ggtgctggtg ttggtgaagt tgttttatat gcttctggtt cttctgctag acaaactgaa     7020

gttactaata atagaccagt tgatgctact attatggcta ttgttgattt ggttgaaatg     7080

ggtggtgatg ttagatttag aaaagatggt tcttcttggt cacatccaca atttgaaaag     7140

tagcaactaa gctggttcta actggaaata atttccatta gattcctctt tttctcgtcc     7200

attaaccaaa atatattatt gaattcagcg gttccttttt tctcattttc gcatatagct     7260

gcactattag aatcagccca ctctaggtaa acacagttcc tcgatatacc tctgtcttac     7320

tatcagtggt taaaccttat gcaaatataa tatatatata tatatatata tatatatctc     7380

atacttttgt tgattcttgt gtaattattg gaaaagacaa aacaaagcaa gcgtttctat     7440

tcatatttac aagtattttt tatgacaaac tatttcttaa ttttcccacc ggcggctttg     7500

aataaggcaa tgtcattgtc ctgcataata tattgtttgc ctgcacgttt gataagtccc     7560

ttagatttta gtaaagactc atttagcggt ggttccatct tccctc                    7606


<210>  77
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter Pcon3 nucleotide sequence

<400>  77
ggctggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tcctgacagc       60

tagctcagtc ctaggtataa tgctagcgaa ttcaaaagat cttttaagaa ggagatatac      120

at                                                                     122


<210>  78
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter Pcon4 nucleotide sequence

<400>  78
ggctggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tctttacggc       60

tagctcagtc ctaggtacta tgctagcgaa ttcaaaagat cttttaagaa ggagatatac      120

at                                                                     122


<210>  79
<211>  154
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter TT7 nucleotide sequence

<400>  79
tagctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag       60

cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta      120

tatccggata tcccgcaaga ggcccggcag tacc                                  154


<210>  80
<211>  459
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yeast transcriptional terminator TRPL41B

<400>  80
tagcgcggat tgagagcaaa tcgttaagtt caggtcaagt aaaaattgat ttcgaaaact       60

aatttctctt atacaatcct ttgattggac cgtcatcctt tcgaatataa gattttgtta      120

agaatatttt agacagagat ctactttata tttaatatct agatattaca taatttcctc      180

tctaataaaa tatcattaat aaaataaaaa tgaagcgatt tgattttgtg ttgtcaactt      240

agtttgccgc tatgcctctt gggtaatgct attattgaat cgaagggctt tattatatta      300

ccctttagct tattctgagg tttctgtggc gtgcaaagtg atgaaccggg cgggttttaa      360

ggataaaatc aaaaagtgaa aaaatgaacg gaaaatggaa tacctgtgaa atggagaatg      420

ataatgaatc tttctgtcgt gcttgaaaga ttttcggct                             459


<210>  81
<211>  430
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yeast transcriptional terminator THBT1

<400>  81
tagcacactt ctcgattaac aaattcccag tattctttga aatctatttt tcttcctcaa       60

ttgaatttga ataactgtct acgcggactc ctcctatcta caactacaac aaattttaac      120

cactttatta ccactttcct ctttcattta tttttgtctt ttatgttgtc aatttactag      180

tatttttttt tttttcattt acgttcaagg ttttttatac tcatttaact tgtcttaggt      240

tatttatata tatacctata tatttatata tatatatata tatgtatgta tatattatta      300

tcaccaaatg agaaataata gctaatttga tttttgatta tttaaaatat tggtttgttc      360

tttctgcaaa catctcgttt ggtacgatat tagtgaaaaa cgatgtaatt atcaacacgt      420

gcattaccca                                                             430


<210>  82
<211>  458
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yeast transcriptional terminator TRPS20

<400>  82
tagcaactaa gctggttcta actggaaata atttccatta gattcctctt tttctcgtcc       60

attaaccaaa atatattatt gaattcagcg gttccttttt tctcattttc gcatatagct      120

gcactattag aatcagccca ctctaggtaa acacagttcc tcgatatacc tctgtcttac      180

tatcagtggt taaaccttat gcaaatataa tatatatata tatatatata tctcatactt      240

ttgttgattc ttgtgtaatt attggaaaag acaaaacaaa gcaagcgttt ctattcatca      300

tatttacaag tatttttatg aaaaactatt tcttaatttt cccaccggcg gctttgaata      360

aggcaatgtc attgtcctgc ataatatatt gtttgcctgc acgtttgata agtcccttag      420

attttagtaa agactcattt agcggtggtt ccatcttc                              458


<210>  83
<211>  1864
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-Pcon2 plasmid

<400>  83
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctggcttcc caaccttacc agagggcgcc ccagctggca attccgacgt      120

cttgacggct agctcagtcc taggtacagt gctagcgaat tcaaaagatc ttttaagaag      180

gagatataca tgatgcgaga cgactgacca tttaaatcat acctgacctc catagcagaa      240

agtcaaaagc ctccgaccgg aggcttttga cttgatcggc acgtaagagg ttccaacttt      300

caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga      360

gctaaggaag ctaaaatgag ccatattcaa cgggaaacgt cttgctcgag gccgcgatta      420

aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa      480

tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa      540

catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcaggct aaactggctg      600

acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg      660

ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga atatcctgat      720

tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct      780

gtttgtaatt gtccttttaa cggcgatcgc gtatttcgtc tcgctcaggc gcaatcacga      840

atgaataacg gtttggttgg tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt      900

gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact      960

catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt     1020

gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc     1080

ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat     1140

cctgatatga ataaattgca gtttcacttg atgctcgatg agtttttcta atgagggccc     1200

aaatgtaatc acctggctca ccttcgggtg ggcctttctg cgttgctggc gtttttccat     1260

aggctccgcc cccctgacga gcatcacaaa aatcgatgct caagtcagag gtggcgaaac     1320

ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct     1380

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     1440

ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     1500

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     1560

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     1620

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     1680

ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt tacctcggaa     1740

aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg     1800

tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgattttct     1860

accg                                                                  1864


<210>  84
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter Pcon2 nucleotide sequence

<400>  84
ggctggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tcttgacggc       60

tagctcagtc ctaggtacag tgctagcgaa ttcaaaagat cttttaagaa ggagatatac      120

at                                                                     122


<210>  85
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_chc_F' forward primer

<400>  85
gatcctttga ttttctaccg                                                   20


<210>  86
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_chc_R' reverse primer

<400>  86
ctcgataact caaaaaatac g                                                 21


<210>  87
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES_Chc_F' forward primer

<400>  87
cggagcctat ggaaaaacgc                                                   20


<210>  88
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pES_Chc_R' reverse primer

<400>  88
ccgcagtgtc ttgggtctct                                                   20


<210>  89
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His_chc_F' forward primer

<400>  89
tagagtgtac tagaggaggc caa                                               23


<210>  90
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CEN_chc_R' reverse primer

<400>  90
ggtgatgacg gtgaaaacct                                                   20


<210>  91
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ura_chc_F' forward primer

<400>  91
tctgttcgga gattaccgaa tcaa                                              24


<210>  92
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGau_chc_F' forward primer

<400>  92
ccacctcagg cagagaacct                                                   20


<210>  93
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGau_chc_R' reverse primer

<400>  93
ggaaaaacgc cagcaacgc                                                    19


<210>  94
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  S2CP(30) amino acid sequence

<400>  94

Lys Pro Glu Lys Pro Gly Ser Lys Ile Thr Gly Ser Ser Gly Asn Asp 
1               5                   10                  15      


Thr Gln Gly Ser Leu Ile Thr Tyr Ser Gly Gly Ala Arg Gly 
            20                  25                  30  


<210>  95
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S2CP(30) nucleotide sequence

<400>  95
aaaccggaaa aaccaggtag caaaattacc ggtagcagcg gcaatgatac ccagggtagc       60

ctgattacct atagcggtgg tgcacgtggt                                        90


<210>  96
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HO-T1-SpyTag amino acid sequence

<400>  96

Met Asp His Ala Pro Glu Arg Phe Asp Ala Thr Pro Pro Ala Gly Glu 
1               5                   10                  15      


Pro Asp Arg Pro Ala Leu Gly Val Leu Glu Leu Thr Ser Ile Ala Arg 
            20                  25                  30          


Gly Ile Thr Val Ala Asp Ala Ala Leu Lys Arg Ala Pro Ser Leu Leu 
        35                  40                  45              


Leu Met Ser Arg Pro Val Ser Ser Gly Lys His Leu Leu Met Met Arg 
    50                  55                  60                  


Gly Gln Val Ala Glu Val Glu Glu Ser Met Ile Ala Ala Arg Glu Ile 
65                  70                  75                  80  


Ala Gly Ala Gly Gly Gly Ser Gly Gly Ser Ala His Ile Val Met Val 
                85                  90                  95      


Asp Ala Tyr Lys Pro Thr Lys Gly Gly Ser Gly Gly Ser Gly Ala Leu 
            100                 105                 110         


Leu Asp Glu Leu Glu Leu Pro Tyr Ala His Glu Gln Leu Trp Arg Phe 
        115                 120                 125             


Leu Asp Ala Pro Val Val Ala Asp Ala Trp Glu Glu Asp Thr Glu Ser 
    130                 135                 140                 


Val Ile Ile Val Glu Thr Ala Thr Val Cys Ala Ala Ile Asp Ser Ala 
145                 150                 155                 160 


Asp Ala Ala Leu Lys Thr Ala Pro Val Val Leu Arg Asp Met Arg Leu 
                165                 170                 175     


Ala Ile Gly Ile Ala Gly Lys Ala Phe Phe Thr Leu Thr Gly Glu Leu 
            180                 185                 190         


Ala Asp Val Glu Ala Ala Ala Glu Val Val Arg Glu Arg Cys Gly Ala 
        195                 200                 205             


Arg Leu Leu Glu Leu Ala Cys Ile Ala Arg Pro Val Asp Glu Leu Arg 
    210                 215                 220                 


Gly Arg Leu Phe Phe 
225                 


<210>  97
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HO-T1-SpyTag nucleotide sequence

<400>  97
atggatcatg ctccagaaag atttgatgct actcctccag ctggtgaacc agatagacca       60

gctttgggtg ttttggaatt gacttctatt gctagaggta ttaccgttgc tgatgctgct      120

ttgaaaagag caccatcttt gttgttgatg tccagaccag tttcttccgg taaacatttg      180

ttgatgatga gaggtcaagt tgccgaagtt gaagaatcta tgattgctgc tagagaaatt      240

gctggtgctg gtggtggttc aggtggttct gctcatatag ttatggttga tgcttacaag      300

ccaacaaaag gtggtagtgg tggatctggt gctttgttgg atgaattgga attgccatat      360

gctcacgaac aactttggag atttttggat gctccagttg ttgcagatgc ttgggaagaa      420

gatactgaat ccgttattat cgttgaaacc gctactgttt gtgctgctat tgattctgct      480

gatgcagcct taaaaactgc tcctgttgtt ttgagagata tgagattggc tattggtatt      540

gctggtaagg ctttctttac tttgactggt gaattggctg atgttgaagc tgctgctgaa      600

gttgttagag aaagatgtgg tgctagattg ctagaattgg catgtattgc aagaccagtt      660

gacgaattga gaggtaggtt gtttttc                                          687


<210>  98
<211>  339
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP-SpyCatcher amino acid sequence

<400>  98

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Ser Lys 
1               5                   10                  15      


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
            20                  25                  30          


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
        35                  40                  45              


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
    50                  55                  60                  


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
65                  70                  75                  80  


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
                85                  90                  95      


Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe 
            100                 105                 110         


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
        115                 120                 125             


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
    130                 135                 140                 


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser 
145                 150                 155                 160 


His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Val 
                165                 170                 175     


Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala 
            180                 185                 190         


Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu 
        195                 200                 205             


Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Lys Leu Ser Lys Asp Pro 
    210                 215                 220                 


Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala 
225                 230                 235                 240 


Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Gly Ser Gly Gly Ser 
                245                 250                 255     


Asp Ser Ala Thr His Ile Lys Phe Ser Lys Arg Asp Glu Asp Gly Lys 
            260                 265                 270         


Glu Leu Ala Gly Ala Thr Met Glu Leu Arg Asp Ser Ser Gly Lys Thr 
        275                 280                 285             


Ile Ser Thr Trp Ile Ser Asp Gly Gln Val Lys Asp Phe Tyr Leu Tyr 
    290                 295                 300                 


Pro Gly Lys Tyr Thr Phe Val Glu Thr Ala Ala Pro Asp Gly Tyr Glu 
305                 310                 315                 320 


Val Ala Thr Ala Ile Thr Phe Thr Val Asn Glu Gln Gly Gln Val Thr 
                325                 330                 335     


Val Asn Gly 
            


<210>  99
<211>  1017
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GFP-SpyCatcher nucleotide sequence

<400>  99
atgggttctt ctcatcatca ccatcaccat tcttctggga tgtctaaagg tgaagaatta       60

ttcactggtg ttgtcccaat tttggttgaa ttagatggtg atgttaatgg tcacaaattt      120

tctgtctccg gtgaaggtga aggtgatgct acttacggta aattgacctt aaaatttatt      180

tgtactactg gtaaattgcc agttccatgg ccaaccttag tcactacttt aacttatggt      240

gttcaatgtt tttctagata cccagatcat atgaaacaac atgacttttt caagtctgcc      300

atgccagaag gttatgttca agaaagaact atttttttca aagatgacgg taactacaag      360

accagagctg aagtcaagtt tgaaggtgat accttagtta atagaatcga attaaaaggt      420

attgatttta aagaagatgg taacatttta ggtcacaaat tggaatacaa ctataactct      480

cacaatgttt acatcatggc tgacaaacaa aagaatggta tcaaagttaa cttcaaaatt      540

agacacaaca ttgaagatgg ttctgttcaa ttagctgacc attatcaaca aaatactcca      600

attggtgatg gtccagtctt gttaccagac aaccattact tatccactca atctaaatta      660

tccaaagatc caaacgaaaa gagagatcac atggtcttgt tagaatttgt tactgctgct      720

ggtattaccc atggtatgga tgaattgtac aaaggttctg gtggttctga ttctgctact      780

catattaagt tctccaagag ggacgaagat ggtaaagaat tggctggtgc aactatggaa      840

ttgagagatt cttctggtaa gaccatttcc acctggattt ctgatggtca agttaaggat      900

ttctacttgt acccaggtaa gtacactttc gttgaaactg ctgctccaga tggttatgaa      960

gttgctactg ctattacttt caccgtcaat gaacaaggtc aagtcactgt taatggt        1017


<210>  100
<211>  296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  APEX2-S2CP(30) amino acid sequence

<400>  100

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Gly Lys 
1               5                   10                  15      


Ser Tyr Pro Thr Val Ser Ala Asp Tyr Gln Asp Ala Val Glu Lys Ala 
            20                  25                  30          


Lys Lys Lys Leu Arg Gly Phe Ile Ala Glu Lys Arg Cys Ala Pro Leu 
        35                  40                  45              


Met Leu Arg Leu Ala Phe His Ser Ala Gly Thr Phe Asp Lys Gly Thr 
    50                  55                  60                  


Lys Thr Gly Gly Pro Phe Gly Thr Ile Lys His Pro Ala Glu Leu Ala 
65                  70                  75                  80  


His Ser Ala Asn Asn Gly Leu Asp Ile Ala Val Arg Leu Leu Glu Pro 
                85                  90                  95      


Leu Lys Ala Glu Phe Pro Ile Leu Ser Tyr Ala Asp Phe Tyr Gln Leu 
            100                 105                 110         


Ala Gly Val Val Ala Val Glu Val Thr Gly Gly Pro Lys Val Pro Phe 
        115                 120                 125             


His Pro Gly Arg Glu Asp Lys Pro Glu Pro Pro Pro Glu Gly Arg Leu 
    130                 135                 140                 


Pro Asp Pro Thr Lys Gly Ser Asp His Leu Arg Asp Val Phe Gly Lys 
145                 150                 155                 160 


Ala Met Gly Leu Thr Asp Gln Asp Ile Val Ala Leu Ser Gly Gly His 
                165                 170                 175     


Thr Ile Gly Ala Ala His Lys Glu Arg Ser Gly Phe Glu Gly Pro Trp 
            180                 185                 190         


Thr Ser Asn Pro Leu Ile Phe Asp Asn Ser Tyr Phe Thr Glu Leu Leu 
        195                 200                 205             


Ser Gly Glu Lys Glu Gly Leu Leu Gln Leu Pro Ser Asp Lys Ala Leu 
    210                 215                 220                 


Leu Ser Asp Pro Val Phe Arg Pro Leu Val Asp Lys Tyr Ala Ala Asp 
225                 230                 235                 240 


Glu Asp Ala Phe Phe Ala Asp Tyr Ala Glu Ala His Gln Lys Leu Ser 
                245                 250                 255     


Glu Leu Gly Phe Ala Asp Ala Gly Ser Ser Lys Pro Glu Lys Pro Gly 
            260                 265                 270         


Ser Lys Ile Thr Gly Ser Ser Gly Asn Asp Thr Gln Gly Ser Leu Ile 
        275                 280                 285             


Thr Tyr Ser Gly Gly Ala Arg Gly 
    290                 295     


<210>  101
<211>  888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  APEX2-S2CP(30) nucleotide sequence

<400>  101
atgggttctt ctcatcatca ccatcaccat tcttctggga tgggtaagtc ttacccaact       60

gtttctgctg attatcaaga tgctgttgaa aaggccaaga agaagttgag aggtttcatt      120

gctgaaaaaa gatgcgctcc attgatgttg agattggctt ttcattctgc tggtactttc      180

gataagggta caaaaactgg tggtccattc ggtactatca aacatccagc tgaattggct      240

cattcagcta acaatggttt ggatattgct gtcagattgc tggaaccatt gaaagccgaa      300

tttccaattt tgtcctacgc cgatttttac caattggctg gtgttgttgc agttgaagtt      360

acaggtggtc caaaagttcc atttcatcca ggtagagaag ataagccaga accaccacca      420

gaaggtagat tgccagatcc aacaaaaggt tctgatcact tgagagatgt tttcggtaaa      480

gctatgggtt tgactgatca agatattgtc gctttgtctg gtggtcatac aattggtgct      540

gctcacaaag aaagatcagg ttttgaaggt ccttggactt ctaacccatt gatctttgat      600

aactcttact tcaccgagtt gttgtccggt gaaaaagaag gtttgttgca attgccatct      660

gataaggctt tgttgtctga tccagttttc agaccattgg ttgataagta tgctgctgat      720

gaagatgctt tctttgctga ttacgctgaa gctcatcaaa agttgtctga attgggtttt      780

gctgatgctg gttcttctaa accggaaaaa ccaggtagca aaattaccgg tagcagcggc      840

aatgataccc agggtagcct gattacctat agcggtggtg cacgtggt                   888


<210>  102
<211>  1070
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LacZ-S2CP(30) amino acid sequence

<400>  102

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Thr Met 
1               5                   10                  15      


Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn 
            20                  25                  30          


Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala 
        35                  40                  45              


Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln 
    50                  55                  60                  


Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe Pro Ala Pro 
65                  70                  75                  80  


Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro Glu Ala Asp 
                85                  90                  95      


Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly Tyr Asp Ala Pro 
            100                 105                 110         


Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro Pro Phe Val 
        115                 120                 125             


Pro Thr Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr Phe Asn Val Asp 
    130                 135                 140                 


Glu Ser Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile Phe Asp Gly Val 
145                 150                 155                 160 


Asn Ser Ala Phe His Leu Trp Cys Asn Gly Arg Trp Val Gly Tyr Gly 
                165                 170                 175     


Gln Asp Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser Ala Phe Leu Arg 
            180                 185                 190         


Ala Gly Glu Asn Arg Leu Ala Val Met Val Leu Arg Trp Ser Asp Gly 
        195                 200                 205             


Ser Tyr Leu Glu Asp Gln Asp Met Trp Arg Met Ser Gly Ile Phe Arg 
    210                 215                 220                 


Asp Val Ser Leu Leu His Lys Pro Thr Thr Gln Ile Ser Asp Phe His 
225                 230                 235                 240 


Val Ala Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala Val Leu Glu Ala 
                245                 250                 255     


Glu Val Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu Arg Val Thr Val 
            260                 265                 270         


Ser Leu Trp Gln Gly Glu Thr Gln Val Ala Ser Gly Thr Ala Pro Phe 
        275                 280                 285             


Gly Gly Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala Asp Arg Val Thr 
    290                 295                 300                 


Leu Arg Leu Asn Val Glu Asn Pro Lys Leu Trp Ser Ala Glu Ile Pro 
305                 310                 315                 320 


Asn Leu Tyr Arg Ala Val Val Glu Leu His Thr Ala Asp Gly Thr Leu 
                325                 330                 335     


Ile Glu Ala Glu Ala Cys Asp Val Gly Phe Arg Glu Val Arg Ile Glu 
            340                 345                 350         


Asn Gly Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile Arg Gly Val 
        355                 360                 365             


Asn Arg His Glu His His Pro Leu His Gly Gln Val Met Asp Glu Gln 
    370                 375                 380                 


Thr Met Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn Phe Asn Ala 
385                 390                 395                 400 


Val Arg Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr Thr Leu Cys 
                405                 410                 415     


Asp Arg Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile Glu Thr His 
            420                 425                 430         


Gly Met Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg Trp Leu Pro 
        435                 440                 445             


Ala Met Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp Arg Asn His 
    450                 455                 460                 


Pro Ser Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly His Gly Ala 
465                 470                 475                 480 


Asn His Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp Pro Ser Arg 
                485                 490                 495     


Pro Val Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile 
            500                 505                 510         


Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro Phe Pro Ala 
        515                 520                 525             


Val Pro Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro Gly Glu Thr 
    530                 535                 540                 


Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly Asn Ser Leu 
545                 550                 555                 560 


Gly Gly Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr Pro Arg Leu 
                565                 570                 575     


Gln Gly Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu Ile Lys Tyr 
            580                 585                 590         


Asp Glu Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly Asp Phe Gly Asp 
        595                 600                 605             


Thr Pro Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val Phe Ala Asp 
    610                 615                 620                 


Arg Thr Pro His Pro Ala Leu Thr Glu Ala Lys His Gln Gln Gln Phe 
625                 630                 635                 640 


Phe Gln Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr Ser Glu Tyr 
                645                 650                 655     


Leu Phe Arg His Ser Asp Asn Glu Leu Leu His Trp Met Val Ala Leu 
            660                 665                 670         


Asp Gly Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp Val Ala Pro 
        675                 680                 685             


Gln Gly Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln Pro Glu Ser 
    690                 695                 700                 


Ala Gly Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro Asn Ala Thr 
705                 710                 715                 720 


Ala Trp Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln Trp Arg Leu 
                725                 730                 735     


Ala Glu Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His Ala Ile Pro 
            740                 745                 750         


His Leu Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu Gly Asn Lys 
        755                 760                 765             


Arg Trp Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln Met Trp Ile 
    770                 775                 780                 


Gly Asp Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln Phe Thr Arg 
785                 790                 795                 800 


Ala Pro Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr Arg Ile Asp 
                805                 810                 815     


Pro Asn Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His Tyr Gln Ala 
            820                 825                 830         


Glu Ala Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala Asp Ala Val 
        835                 840                 845             


Leu Ile Thr Thr Ala His Ala Trp Gln His Gln Gly Lys Thr Leu Phe 
    850                 855                 860                 


Ile Ser Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln Met Ala Ile 
865                 870                 875                 880 


Thr Val Asp Val Glu Val Ala Ser Asp Thr Pro His Pro Ala Arg Ile 
                885                 890                 895     


Gly Leu Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val Asn Trp Leu 
            900                 905                 910         


Gly Leu Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr Ala Ala Cys 
        915                 920                 925             


Phe Asp Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr Pro Tyr Val 
    930                 935                 940                 


Phe Pro Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu Leu Asn Tyr 
945                 950                 955                 960 


Gly Pro His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile Ser Arg Tyr 
                965                 970                 975     


Ser Gln Gln Gln Leu Met Glu Thr Ser His Arg His Leu Leu His Ala 
            980                 985                 990         


Glu Glu Gly Thr Trp Leu Asn Ile  Asp Gly Phe His Met  Gly Ile Gly 
        995                 1000                 1005             


Gly Asp  Asp Ser Trp Ser Pro  Ser Val Ser Ala Glu  Phe Gln Leu 
    1010                 1015                 1020             


Ser Ala  Gly Arg Tyr His Tyr  Gln Leu Val Trp Cys  Gln Lys Gly 
    1025                 1030                 1035             


Ser Ser  Lys Pro Glu Lys Pro  Gly Ser Lys Ile Thr  Gly Ser Ser 
    1040                 1045                 1050             


Gly Asn  Asp Thr Gln Gly Ser  Leu Ile Thr Tyr Ser  Gly Gly Ala 
    1055                 1060                 1065             


Arg Gly  
    1070 


<210>  103
<211>  3210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LacZ-S2CP(30) nucleotide sequence

<400>  103
atgggttctt ctcatcatca ccatcaccat tcttctggga tgaccatgat tacggattca       60

ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc      120

cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc      180

ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca      240

gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc      300

ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc      360

attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca      420

tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt      480

aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt      540

ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg      600

atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc      660

ggcattttcc gtgatgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat      720

gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg      780

tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag      840

gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc      900

gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg      960

aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa     1020

gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc     1080

aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc     1140

atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc     1200

gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc     1260

ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg     1320

accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc     1380

gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct     1440

aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat     1500

gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg     1560

gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta     1620

cctggagaaa cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt     1680

ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc     1740

gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg     1800

gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg     1860

gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt     1920

ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat     1980

agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt     2040

gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg     2100

cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc     2160

gcatggtcag aagccggaca catcagcgcc tggcagcagt ggcgtctggc tgaaaacctc     2220

agcgtgacac tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat     2280

ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca     2340

cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt     2400

gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg     2460

gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg     2520

gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg     2580

aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt     2640

accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc     2700

cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat     2760

cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat     2820

accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat     2880

ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa     2940

ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc     3000

gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa     3060

ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa aggttcttct     3120

aaaccggaaa aaccaggtag caaaattacc ggtagcagcg gcaatgatac ccagggtagc     3180

ctgattacct atagcggtgg tgcacgtggt                                      3210


<210>  104
<211>  453
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Promoter PGPM1 nucleotide sequence

<400>  104
gtgatgtcta agtaaccttt atggtatatt tcttaatgtg gaaagatact agcgcgcgca       60

cccacacaca agcttcgtct tttcttgaag aaaagaggaa gctcgctaaa tgggattcca      120

ctttccgttc cctgccagct gatggaaaaa ggttagtgga acgatgaaga ataaaaagag      180

agatccactg aggtgaaatt tcagctgaca gcgagtttca tgatcgtgat gaacaatggt      240

aacgagttgt ggctgttgcc agggagggtg gttctcaact tttaatgtat ggccaaatcg      300

ctacttgggt ttgttatata acaaagaaga aataatgaac tgattctctt cctccttctt      360

gtcctttctt aattctgttg taattacctt cctttgtaat tttttttgta attattcttc      420

ttaataatcc aaacaaacac acatattaca ata                                   453


<210>  105
<211>  432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yeast transcriptional terminator TYPT31

<400>  105
gagatatttt gcagcagttg cgcacttgca tgtgaatgac tcttctcccc tttaattctg       60

tgctatattt ttacaatttt ctgctgacat atagtttata tacatataga acgcatatag      120

gaaattgaag taaacagaat acacaagtag aggccggtat gtacgacatt ttgcttacta      180

ctctttaaaa tcatcgtctt cttcgtcttc atcgtcttct tctttttcac catatcctac      240

atcatcttta gagcctgtgc taggttcctt cttgtctaat tcttctgcag tctttttata      300

gtcaattact ttgccgcgtg ttcttcttcc ggatgtgatg atattagagg tatcaatttc      360

tgccaaatcg tcctcttctt cttctccctc atttcccatc aatgcgtcta acttggcatc      420

gtccatatca ga                                                          432


<210>  106
<211>  2971
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-S2CP(30) plasmid

<400>  106
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgagagacc gaattcgcgg ccgcttctag agcaatacgc aaaccgcctc      120

tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag      180

cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt      240

tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca      300

catactagag aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt      360

catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg      420

tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg      480

tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc      540

ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt      600

caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc      660

ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc      720

cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta      780

cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg      840

tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg      900

tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact acaccatcgt      960

tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg     1020

ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac     1080

tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc     1140

tcaccttcgg gtgggccttt ctgcgtttat atactagtag cggccgctgc agggtctctg     1200

gttcttctaa accggaaaaa ccaggtagca aaattaccgg tagcagcggc aatgataccc     1260

agggtagcct gattacctat agcggtggtg cacgtggtta gccgagacga ctgaccattt     1320

aaatcatacc tgacctccat agcagaaagt caaaagcctc cgaccggagg cttttgactt     1380

gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta     1440

ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca tattcaacgg     1500

gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat     1560

aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag     1620

cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca     1680

gatgagatgg tcaggctaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat     1740

tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg gaaaacagca     1800

ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg     1860

ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacgg cgatcgcgta     1920

tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttggtgc gagtgatttt     1980

gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taagcttttg     2040

ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt     2100

gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac     2160

caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg     2220

ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcacttgatg     2280

ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc tggctcacct tcgggtgggc     2340

ctttctgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat     2400

cgatgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc     2460

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc     2520

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt     2580

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac     2640

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg     2700

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca     2760

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc     2820

gctctgctga agccagttac ctcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     2880

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     2940

gatctcaaga agatcctttg attttctacc g                                    2971


<210>  107
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-S2CP(30) nucleotide sequence of key ORF

<400>  107
aaaccggaaa aaccaggtag caaaattacc ggtagcagcg gcaatgatac ccagggtagc       60

ctgattacct atagcggtgg tgcacgtggt                                        90


<210>  108
<211>  36
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-S2CP(30) amino acid sequence of key ORF

<400>  108

Lys Pro Glu Lys Pro Gly Lys Pro Glu Lys Pro Gly Ser Lys Ile Thr 
1               5                   10                  15      


Gly Ser Ser Gly Asn Asp Thr Gln Gly Ser Leu Ile Thr Tyr Ser Gly 
            20                  25                  30          


Gly Ala Arg Gly 
        35      


<210>  109
<211>  2631
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-APEX2-S2CP(30) plasmid

<400>  109
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgggttctt ctcatcatca ccatcaccat tcttctggga tgggtaagtc      120

ttacccaact gtttctgctg attatcaaga tgctgttgaa aaggccaaga agaagttgag      180

aggtttcatt gctgaaaaaa gatgcgctcc attgatgttg agattggctt ttcattctgc      240

tggtactttc gataagggta caaaaactgg tggtccattc ggtactatca aacatccagc      300

tgaattggct cattcagcta acaatggttt ggatattgct gtcagattgc tggaaccatt      360

gaaagccgaa tttccaattt tgtcctacgc cgatttttac caattggctg gtgttgttgc      420

agttgaagtt acaggtggtc caaaagttcc atttcatcca ggtagagaag ataagccaga      480

accaccacca gaaggtagat tgccagatcc aacaaaaggt tctgatcact tgagagatgt      540

tttcggtaaa gctatgggtt tgactgatca agatattgtc gctttgtctg gtggtcatac      600

aattggtgct gctcacaaag aaagatcagg ttttgaaggt ccttggactt ctaacccatt      660

gatctttgat aactcttact tcaccgagtt gttgtccggt gaaaaagaag gtttgttgca      720

attgccatct gataaggctt tgttgtctga tccagttttc agaccattgg ttgataagta      780

tgctgctgat gaagatgctt tctttgctga ttacgctgaa gctcatcaaa agttgtctga      840

attgggtttt gctgatgctg gttcttctaa accggaaaaa ccaggtagca aaattaccgg      900

tagcagcggc aatgataccc agggtagcct gattacctat agcggtggtg cacgtggtta      960

gccgagacga ctgaccattt aaatcatacc tgacctccat agcagaaagt caaaagcctc     1020

cgaccggagg cttttgactt gatcggcacg taagaggttc caactttcac cataatgaaa     1080

taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta     1140

aaatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg     1200

atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa     1260

tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta     1320

gcgttgccaa tgatgttaca gatgagatgg tcaggctaaa ctggctgacg gaatttatgc     1380

ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg     1440

cgatcccagg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata     1500

ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc     1560

cttttaacgg cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt     1620

tggttggtgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga     1680

aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct     1740

cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag     1800

tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt     1860

ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata     1920

aattgcagtt tcacttgatg ctcgatgagt ttttctaatg agggcccaaa tgtaatcacc     1980

tggctcacct tcgggtgggc ctttctgcgt tgctggcgtt tttccatagg ctccgccccc     2040

ctgacgagca tcacaaaaat cgatgctcaa gtcagaggtg gcgaaacccg acaggactat     2100

aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc     2160

cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct     2220

cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg     2280

aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc     2340

cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga     2400

ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa     2460

gaacagtatt tggtatctgc gctctgctga agccagttac ctcggaaaaa gagttggtag     2520

ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca     2580

gattacgcgc agaaaaaaag gatctcaaga agatcctttg attttctacc g              2631


<210>  110
<211>  888
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-APEX2-S2CP(30) nucleotide sequence of key ORF

<400>  110
atgggttctt ctcatcatca ccatcaccat tcttctggga tgggtaagtc ttacccaact       60

gtttctgctg attatcaaga tgctgttgaa aaggccaaga agaagttgag aggtttcatt      120

gctgaaaaaa gatgcgctcc attgatgttg agattggctt ttcattctgc tggtactttc      180

gataagggta caaaaactgg tggtccattc ggtactatca aacatccagc tgaattggct      240

cattcagcta acaatggttt ggatattgct gtcagattgc tggaaccatt gaaagccgaa      300

tttccaattt tgtcctacgc cgatttttac caattggctg gtgttgttgc agttgaagtt      360

acaggtggtc caaaagttcc atttcatcca ggtagagaag ataagccaga accaccacca      420

gaaggtagat tgccagatcc aacaaaaggt tctgatcact tgagagatgt tttcggtaaa      480

gctatgggtt tgactgatca agatattgtc gctttgtctg gtggtcatac aattggtgct      540

gctcacaaag aaagatcagg ttttgaaggt ccttggactt ctaacccatt gatctttgat      600

aactcttact tcaccgagtt gttgtccggt gaaaaagaag gtttgttgca attgccatct      660

gataaggctt tgttgtctga tccagttttc agaccattgg ttgataagta tgctgctgat      720

gaagatgctt tctttgctga ttacgctgaa gctcatcaaa agttgtctga attgggtttt      780

gctgatgctg gttcttctaa accggaaaaa ccaggtagca aaattaccgg tagcagcggc      840

aatgataccc agggtagcct gattacctat agcggtggtg cacgtggt                   888


<210>  111
<211>  296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-APEX2-S2CP(30) amino acid sequence of key ORF

<400>  111

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Gly Lys 
1               5                   10                  15      


Ser Tyr Pro Thr Val Ser Ala Asp Tyr Gln Asp Ala Val Glu Lys Ala 
            20                  25                  30          


Lys Lys Lys Leu Arg Gly Phe Ile Ala Glu Lys Arg Cys Ala Pro Leu 
        35                  40                  45              


Met Leu Arg Leu Ala Phe His Ser Ala Gly Thr Phe Asp Lys Gly Thr 
    50                  55                  60                  


Lys Thr Gly Gly Pro Phe Gly Thr Ile Lys His Pro Ala Glu Leu Ala 
65                  70                  75                  80  


His Ser Ala Asn Asn Gly Leu Asp Ile Ala Val Arg Leu Leu Glu Pro 
                85                  90                  95      


Leu Lys Ala Glu Phe Pro Ile Leu Ser Tyr Ala Asp Phe Tyr Gln Leu 
            100                 105                 110         


Ala Gly Val Val Ala Val Glu Val Thr Gly Gly Pro Lys Val Pro Phe 
        115                 120                 125             


His Pro Gly Arg Glu Asp Lys Pro Glu Pro Pro Pro Glu Gly Arg Leu 
    130                 135                 140                 


Pro Asp Pro Thr Lys Gly Ser Asp His Leu Arg Asp Val Phe Gly Lys 
145                 150                 155                 160 


Ala Met Gly Leu Thr Asp Gln Asp Ile Val Ala Leu Ser Gly Gly His 
                165                 170                 175     


Thr Ile Gly Ala Ala His Lys Glu Arg Ser Gly Phe Glu Gly Pro Trp 
            180                 185                 190         


Thr Ser Asn Pro Leu Ile Phe Asp Asn Ser Tyr Phe Thr Glu Leu Leu 
        195                 200                 205             


Ser Gly Glu Lys Glu Gly Leu Leu Gln Leu Pro Ser Asp Lys Ala Leu 
    210                 215                 220                 


Leu Ser Asp Pro Val Phe Arg Pro Leu Val Asp Lys Tyr Ala Ala Asp 
225                 230                 235                 240 


Glu Asp Ala Phe Phe Ala Asp Tyr Ala Glu Ala His Gln Lys Leu Ser 
                245                 250                 255     


Glu Leu Gly Phe Ala Asp Ala Gly Ser Ser Lys Pro Glu Lys Pro Gly 
            260                 265                 270         


Ser Lys Ile Thr Gly Ser Ser Gly Asn Asp Thr Gln Gly Ser Leu Ile 
        275                 280                 285             


Thr Tyr Ser Gly Gly Ala Arg Gly 
    290                 295     


<210>  112
<211>  4953
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-LacZ-S2CTP plasmid

<400>  112
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atgggttctt ctcatcatca ccatcaccat tcttctggga tgaccatgat      120

tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca      180

acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg      240

caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt      300

tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac      360

tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt      420

gacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta      480

ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt      540

tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca      600

ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg      660

cctcgcggtg atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg      720

gcggatgagc ggcattttcc gtgatgtctc gttgctgcat aaaccgacta cacaaatcag      780

cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga      840

agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg      900

tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg      960

tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc     1020

cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat     1080

tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct     1140

gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca     1200

tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa     1260

ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga     1320

ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat     1380

gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat     1440

ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg     1500

ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc     1560

ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta     1620

cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg     1680

gctttcgcta cctggagaaa cgcgcccgct gatcctttgc gaatacgccc acgcgatggg     1740

taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca     1800

gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa     1860

cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat     1920

gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca     1980

gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct     2040

gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct     2100

ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc     2160

tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc     2220

gaacgcgacc gcatggtcag aagccggaca catcagcgcc tggcagcagt ggcgtctggc     2280

tgaaaacctc agcgtgacac tccccgccgc gtcccacgcc atcccgcatc tgaccaccag     2340

cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg     2400

ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca     2460

gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc     2520

taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt     2580

gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca     2640

gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca     2700

aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg     2760

cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca     2820

agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc     2880

agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga     2940

attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag     3000

tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg     3060

gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt     3120

atcggcggaa ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa     3180

aggttcttct aaaccggaaa aaccaggtag caaaattacc ggtagcagcg gcaatgatac     3240

ccagggtagc ctgattacct atagcggtgg tgcacgtggt tagccgagac gactgaccat     3300

ttaaatcata cctgacctcc atagcagaaa gtcaaaagcc tccgaccgga ggcttttgac     3360

ttgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg     3420

tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatgagc catattcaac     3480

gggaaacgtc ttgctcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt     3540

ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga     3600

agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta     3660

cagatgagat ggtcaggcta aactggctga cggaatttat gcctcttccg accatcaagc     3720

attttatccg tactcctgat gatgcatggt tactcaccac tgcgatccca gggaaaacag     3780

cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag     3840

tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac ggcgatcgcg     3900

tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttggt gcgagtgatt     3960

ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataagcttt     4020

tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt     4080

ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat     4140

accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac     4200

ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcacttga     4260

tgctcgatga gtttttctaa tgagggccca aatgtaatca cctggctcac cttcgggtgg     4320

gcctttctgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa     4380

atcgatgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc     4440

cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt     4500

ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca     4560

gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg     4620

accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat     4680

cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta     4740

cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct     4800

gcgctctgct gaagccagtt acctcggaaa aagagttggt agctcttgat ccggcaaaca     4860

aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa     4920

aggatctcaa gaagatcctt tgattttcta ccg                                  4953


<210>  113
<211>  3210
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-LacZ-S2CTP nucleotid sequence of key ORF

<400>  113
atgggttctt ctcatcatca ccatcaccat tcttctggga tgaccatgat tacggattca       60

ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc      120

cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc      180

ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt tccggcacca      240

gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc      300

ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt gacctatccc      360

attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta ctcgctcaca      420

tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt tgatggcgtt      480

aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt      540

ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg      600

atggtgctgc gctggagtga cggcagttat ctggaagatc aggatatgtg gcggatgagc      660

ggcattttcc gtgatgtctc gttgctgcat aaaccgacta cacaaatcag cgatttccat      720

gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga agttcagatg      780

tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg tgaaacgcag      840

gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg tggttatgcc      900

gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg      960

aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat tgaagcagaa     1020

gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc     1080

aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca tggtcaggtc     1140

atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa ctttaacgcc     1200

gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc     1260

ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat gaatcgtctg     1320

accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc     1380

gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg ccacggcgct     1440

aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat     1500

gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta cgcgcgcgtg     1560

gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta     1620

cctggagaaa cgcgcccgct gatcctttgc gaatacgccc acgcgatggg taacagtctt     1680

ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc     1740

gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg     1800

gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat gaacggtctg     1860

gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca gcagcagttt     1920

ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct gttccgtcat     1980

agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct ggcaagcggt     2040

gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc tgaactaccg     2100

cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc gaacgcgacc     2160

gcatggtcag aagccggaca catcagcgcc tggcagcagt ggcgtctggc tgaaaacctc     2220

agcgtgacac tccccgccgc gtcccacgcc atcccgcatc tgaccaccag cgaaatggat     2280

ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg ctttctttca     2340

cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt     2400

gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg     2460

gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt gcagtgcacg     2520

gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca gcatcagggg     2580

aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca aatggcgatt     2640

accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg cctgaactgc     2700

cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca agaaaactat     2760

cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc agacatgtat     2820

accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat     2880

ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag tcaacagcaa     2940

ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg gctgaatatc     3000

gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt atcggcggaa     3060

ttccagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa aggttcttct     3120

aaaccggaaa aaccaggtag caaaattacc ggtagcagcg gcaatgatac ccagggtagc     3180

ctgattacct atagcggtgg tgcacgtggt                                      3210


<210>  114
<211>  1070
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-LacZ-S2CTP amino acid sequence of key ORF

<400>  114

Met Gly Ser Ser His His His His His His Ser Ser Gly Met Thr Met 
1               5                   10                  15      


Ile Thr Asp Ser Leu Ala Val Val Leu Gln Arg Arg Asp Trp Glu Asn 
            20                  25                  30          


Pro Gly Val Thr Gln Leu Asn Arg Leu Ala Ala His Pro Pro Phe Ala 
        35                  40                  45              


Ser Trp Arg Asn Ser Glu Glu Ala Arg Thr Asp Arg Pro Ser Gln Gln 
    50                  55                  60                  


Leu Arg Ser Leu Asn Gly Glu Trp Arg Phe Ala Trp Phe Pro Ala Pro 
65                  70                  75                  80  


Glu Ala Val Pro Glu Ser Trp Leu Glu Cys Asp Leu Pro Glu Ala Asp 
                85                  90                  95      


Thr Val Val Val Pro Ser Asn Trp Gln Met His Gly Tyr Asp Ala Pro 
            100                 105                 110         


Ile Tyr Thr Asn Val Thr Tyr Pro Ile Thr Val Asn Pro Pro Phe Val 
        115                 120                 125             


Pro Thr Glu Asn Pro Thr Gly Cys Tyr Ser Leu Thr Phe Asn Val Asp 
    130                 135                 140                 


Glu Ser Trp Leu Gln Glu Gly Gln Thr Arg Ile Ile Phe Asp Gly Val 
145                 150                 155                 160 


Asn Ser Ala Phe His Leu Trp Cys Asn Gly Arg Trp Val Gly Tyr Gly 
                165                 170                 175     


Gln Asp Ser Arg Leu Pro Ser Glu Phe Asp Leu Ser Ala Phe Leu Arg 
            180                 185                 190         


Ala Gly Glu Asn Arg Leu Ala Val Met Val Leu Arg Trp Ser Asp Gly 
        195                 200                 205             


Ser Tyr Leu Glu Asp Gln Asp Met Trp Arg Met Ser Gly Ile Phe Arg 
    210                 215                 220                 


Asp Val Ser Leu Leu His Lys Pro Thr Thr Gln Ile Ser Asp Phe His 
225                 230                 235                 240 


Val Ala Thr Arg Phe Asn Asp Asp Phe Ser Arg Ala Val Leu Glu Ala 
                245                 250                 255     


Glu Val Gln Met Cys Gly Glu Leu Arg Asp Tyr Leu Arg Val Thr Val 
            260                 265                 270         


Ser Leu Trp Gln Gly Glu Thr Gln Val Ala Ser Gly Thr Ala Pro Phe 
        275                 280                 285             


Gly Gly Glu Ile Ile Asp Glu Arg Gly Gly Tyr Ala Asp Arg Val Thr 
    290                 295                 300                 


Leu Arg Leu Asn Val Glu Asn Pro Lys Leu Trp Ser Ala Glu Ile Pro 
305                 310                 315                 320 


Asn Leu Tyr Arg Ala Val Val Glu Leu His Thr Ala Asp Gly Thr Leu 
                325                 330                 335     


Ile Glu Ala Glu Ala Cys Asp Val Gly Phe Arg Glu Val Arg Ile Glu 
            340                 345                 350         


Asn Gly Leu Leu Leu Leu Asn Gly Lys Pro Leu Leu Ile Arg Gly Val 
        355                 360                 365             


Asn Arg His Glu His His Pro Leu His Gly Gln Val Met Asp Glu Gln 
    370                 375                 380                 


Thr Met Val Gln Asp Ile Leu Leu Met Lys Gln Asn Asn Phe Asn Ala 
385                 390                 395                 400 


Val Arg Cys Ser His Tyr Pro Asn His Pro Leu Trp Tyr Thr Leu Cys 
                405                 410                 415     


Asp Arg Tyr Gly Leu Tyr Val Val Asp Glu Ala Asn Ile Glu Thr His 
            420                 425                 430         


Gly Met Val Pro Met Asn Arg Leu Thr Asp Asp Pro Arg Trp Leu Pro 
        435                 440                 445             


Ala Met Ser Glu Arg Val Thr Arg Met Val Gln Arg Asp Arg Asn His 
    450                 455                 460                 


Pro Ser Val Ile Ile Trp Ser Leu Gly Asn Glu Ser Gly His Gly Ala 
465                 470                 475                 480 


Asn His Asp Ala Leu Tyr Arg Trp Ile Lys Ser Val Asp Pro Ser Arg 
                485                 490                 495     


Pro Val Gln Tyr Glu Gly Gly Gly Ala Asp Thr Thr Ala Thr Asp Ile 
            500                 505                 510         


Ile Cys Pro Met Tyr Ala Arg Val Asp Glu Asp Gln Pro Phe Pro Ala 
        515                 520                 525             


Val Pro Lys Trp Ser Ile Lys Lys Trp Leu Ser Leu Pro Gly Glu Thr 
    530                 535                 540                 


Arg Pro Leu Ile Leu Cys Glu Tyr Ala His Ala Met Gly Asn Ser Leu 
545                 550                 555                 560 


Gly Gly Phe Ala Lys Tyr Trp Gln Ala Phe Arg Gln Tyr Pro Arg Leu 
                565                 570                 575     


Gln Gly Gly Phe Val Trp Asp Trp Val Asp Gln Ser Leu Ile Lys Tyr 
            580                 585                 590         


Asp Glu Asn Gly Asn Pro Trp Ser Ala Tyr Gly Gly Asp Phe Gly Asp 
        595                 600                 605             


Thr Pro Asn Asp Arg Gln Phe Cys Met Asn Gly Leu Val Phe Ala Asp 
    610                 615                 620                 


Arg Thr Pro His Pro Ala Leu Thr Glu Ala Lys His Gln Gln Gln Phe 
625                 630                 635                 640 


Phe Gln Phe Arg Leu Ser Gly Gln Thr Ile Glu Val Thr Ser Glu Tyr 
                645                 650                 655     


Leu Phe Arg His Ser Asp Asn Glu Leu Leu His Trp Met Val Ala Leu 
            660                 665                 670         


Asp Gly Lys Pro Leu Ala Ser Gly Glu Val Pro Leu Asp Val Ala Pro 
        675                 680                 685             


Gln Gly Lys Gln Leu Ile Glu Leu Pro Glu Leu Pro Gln Pro Glu Ser 
    690                 695                 700                 


Ala Gly Gln Leu Trp Leu Thr Val Arg Val Val Gln Pro Asn Ala Thr 
705                 710                 715                 720 


Ala Trp Ser Glu Ala Gly His Ile Ser Ala Trp Gln Gln Trp Arg Leu 
                725                 730                 735     


Ala Glu Asn Leu Ser Val Thr Leu Pro Ala Ala Ser His Ala Ile Pro 
            740                 745                 750         


His Leu Thr Thr Ser Glu Met Asp Phe Cys Ile Glu Leu Gly Asn Lys 
        755                 760                 765             


Arg Trp Gln Phe Asn Arg Gln Ser Gly Phe Leu Ser Gln Met Trp Ile 
    770                 775                 780                 


Gly Asp Lys Lys Gln Leu Leu Thr Pro Leu Arg Asp Gln Phe Thr Arg 
785                 790                 795                 800 


Ala Pro Leu Asp Asn Asp Ile Gly Val Ser Glu Ala Thr Arg Ile Asp 
                805                 810                 815     


Pro Asn Ala Trp Val Glu Arg Trp Lys Ala Ala Gly His Tyr Gln Ala 
            820                 825                 830         


Glu Ala Ala Leu Leu Gln Cys Thr Ala Asp Thr Leu Ala Asp Ala Val 
        835                 840                 845             


Leu Ile Thr Thr Ala His Ala Trp Gln His Gln Gly Lys Thr Leu Phe 
    850                 855                 860                 


Ile Ser Arg Lys Thr Tyr Arg Ile Asp Gly Ser Gly Gln Met Ala Ile 
865                 870                 875                 880 


Thr Val Asp Val Glu Val Ala Ser Asp Thr Pro His Pro Ala Arg Ile 
                885                 890                 895     


Gly Leu Asn Cys Gln Leu Ala Gln Val Ala Glu Arg Val Asn Trp Leu 
            900                 905                 910         


Gly Leu Gly Pro Gln Glu Asn Tyr Pro Asp Arg Leu Thr Ala Ala Cys 
        915                 920                 925             


Phe Asp Arg Trp Asp Leu Pro Leu Ser Asp Met Tyr Thr Pro Tyr Val 
    930                 935                 940                 


Phe Pro Ser Glu Asn Gly Leu Arg Cys Gly Thr Arg Glu Leu Asn Tyr 
945                 950                 955                 960 


Gly Pro His Gln Trp Arg Gly Asp Phe Gln Phe Asn Ile Ser Arg Tyr 
                965                 970                 975     


Ser Gln Gln Gln Leu Met Glu Thr Ser His Arg His Leu Leu His Ala 
            980                 985                 990         


Glu Glu Gly Thr Trp Leu Asn Ile  Asp Gly Phe His Met  Gly Ile Gly 
        995                 1000                 1005             


Gly Asp  Asp Ser Trp Ser Pro  Ser Val Ser Ala Glu  Phe Gln Leu 
    1010                 1015                 1020             


Ser Ala  Gly Arg Tyr His Tyr  Gln Leu Val Trp Cys  Gln Lys Gly 
    1025                 1030                 1035             


Ser Ser  Lys Pro Glu Lys Pro  Gly Ser Lys Ile Thr  Gly Ser Ser 
    1040                 1045                 1050             


Gly Asn  Asp Thr Gln Gly Ser  Leu Ile Thr Tyr Ser  Gly Gly Ala 
    1055                 1060                 1065             


Arg Gly  
    1070 


<210>  115
<211>  2199
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_P-GPM1 plasmid

<400>  115
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg gctgtgatgt ctaagtaacc tttatggtat atttcttaat gtggaaagat      120

actagcgcgc gcacccacac acaagcttcg tcttttcttg aagaaaagag gaagctcgct      180

aaatgggatt ccactttccg ttccctgcca gctgatggaa aaaggttagt ggaacgatga      240

agaataaaaa gagagatcca ctgaggtgaa atttcagctg acagcgagtt tcatgatcgt      300

gatgaacaat ggtaacgagt tgtggctgtt gccagggagg gtggttctca acttttaatg      360

tatggccaaa tcgctacttg ggtttgttat ataacaaaga agaaataatg aactgattct      420

cttcctcctt cttgtccttt cttaattctg ttgtaattac cttcctttgt aatttttttt      480

gtaattattc ttcttaataa tccaaacaaa cacacatatt acaatagatg cgagacgact      540

gaccatttaa atcatacctg acctccatag cagaaagtca aaagcctccg accggaggct      600

tttgacttga tcggcacgta agaggttcca actttcacca taatgaaata agatcactac      660

cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa atgagccata      720

ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat gctgatttat      780

atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt      840

atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg      900

atgttacaga tgagatggtc aggctaaact ggctgacgga atttatgcct cttccgacca      960

tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atcccaggga     1020

aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc     1080

tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacggcg     1140

atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttggtgcga     1200

gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata     1260

agcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc     1320

ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag     1380

accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac     1440

agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc     1500

acttgatgct cgatgagttt ttctaatgag ggcccaaatg taatcacctg gctcaccttc     1560

gggtgggcct ttctgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc     1620

acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg     1680

cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat     1740

acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt     1800

atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc     1860

agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg     1920

acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg     1980

gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg     2040

gtatctgcgc tctgctgaag ccagttacct cggaaaaaga gttggtagct cttgatccgg     2100

caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag     2160

aaaaaaagga tctcaagaag atcctttgat tttctaccg                            2199


<210>  116
<211>  2430
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-T1-SpyTag plasmid

<400>  116
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgg atggatcatg ctccagaaag atttgatgct actcctccag ctggtgaacc      120

agatagacca gctttgggtg ttttggaatt gacttctatt gctagaggta ttaccgttgc      180

tgatgctgct ttgaaaagag caccatcttt gttgttgatg tccagaccag tttcttccgg      240

taaacatttg ttgatgatga gaggtcaagt tgccgaagtt gaagaatcta tgattgctgc      300

tagagaaatt gctggtgctg gtggtggttc aggtggttct gctcatatag ttatggttga      360

tgcttacaag ccaacaaaag gtggtagtgg tggatctggt gctttgttgg atgaattgga      420

attgccatat gctcacgaac aactttggag atttttggat gctccagttg ttgcagatgc      480

ttgggaagaa gatactgaat ccgttattat cgttgaaacc gctactgttt gtgctgctat      540

tgattctgct gatgcagcct taaaaactgc tcctgttgtt ttgagagata tgagattggc      600

tattggtatt gctggtaagg ctttctttac tttgactggt gaattggctg atgttgaagc      660

tgctgctgaa gttgttagag aaagatgtgg tgctagattg ctagaattgg catgtattgc      720

aagaccagtt gacgaattga gaggtaggtt gtttttctag ccgagacgac tgaccattta      780

aatcatacct gacctccata gcagaaagtc aaaagcctcc gaccggaggc ttttgacttg      840

atcggcacgt aagaggttcc aactttcacc ataatgaaat aagatcacta ccgggcgtat      900

tttttgagtt atcgagattt tcaggagcta aggaagctaa aatgagccat attcaacggg      960

aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta tatgggtata     1020

aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgattg tatgggaagc     1080

ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat gatgttacag     1140

atgagatggt caggctaaac tggctgacgg aatttatgcc tcttccgacc atcaagcatt     1200

ttatccgtac tcctgatgat gcatggttac tcaccactgc gatcccaggg aaaacagcat     1260

tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt     1320

tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacggc gatcgcgtat     1380

ttcgtctcgc acaggcgcaa tcacgaatga ataacggttt ggttggtgcg agtgattttg     1440

atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat aagcttttgc     1500

cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac cttatttttg     1560

acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca gaccgatacc     1620

aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta cagaaacggc     1680

tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt cacttgatgc     1740

tcgatgagtt tttctaatga gggcccaaat gtaatcacct ggctcacctt cgggtgggcc     1800

tttctgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     1860

gatgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     1920

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     1980

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     2040

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     2100

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     2160

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     2220

agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg     2280

ctctgctgaa gccagttacc tcggaaaaag agttggtagc tcttgatccg gcaaacaaac     2340

caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg     2400

atctcaagaa gatcctttga ttttctaccg                                      2430


<210>  117
<211>  687
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-T1-SpyTag nucleotide sequence of key ORF

<400>  117
atggatcatg ctccagaaag atttgatgct actcctccag ctggtgaacc agatagacca       60

gctttgggtg ttttggaatt gacttctatt gctagaggta ttaccgttgc tgatgctgct      120

ttgaaaagag caccatcttt gttgttgatg tccagaccag tttcttccgg taaacatttg      180

ttgatgatga gaggtcaagt tgccgaagtt gaagaatcta tgattgctgc tagagaaatt      240

gctggtgctg gtggtggttc aggtggttct gctcatatag ttatggttga tgcttacaag      300

ccaacaaaag gtggtagtgg tggatctggt gctttgttgg atgaattgga attgccatat      360

gctcacgaac aactttggag atttttggat gctccagttg ttgcagatgc ttgggaagaa      420

gatactgaat ccgttattat cgttgaaacc gctactgttt gtgctgctat tgattctgct      480

gatgcagcct taaaaactgc tcctgttgtt ttgagagata tgagattggc tattggtatt      540

gctggtaagg ctttctttac tttgactggt gaattggctg atgttgaagc tgctgctgaa      600

gttgttagag aaagatgtgg tgctagattg ctagaattgg catgtattgc aagaccagtt      660

gacgaattga gaggtaggtt gtttttc                                          687


<210>  118
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HcKan_O-HO-T1-SpyTag amino acid sequence of key ORF

<400>  118

Met Asp His Ala Pro Glu Arg Phe Asp Ala Thr Pro Pro Ala Gly Glu 
1               5                   10                  15      


Pro Asp Arg Pro Ala Leu Gly Val Leu Glu Leu Thr Ser Ile Ala Arg 
            20                  25                  30          


Gly Ile Thr Val Ala Asp Ala Ala Leu Lys Arg Ala Pro Ser Leu Leu 
        35                  40                  45              


Leu Met Ser Arg Pro Val Ser Ser Gly Lys His Leu Leu Met Met Arg 
    50                  55                  60                  


Gly Gln Val Ala Glu Val Glu Glu Ser Met Ile Ala Ala Arg Glu Ile 
65                  70                  75                  80  


Ala Gly Ala Gly Gly Gly Ser Gly Gly Ser Ala His Ile Val Met Val 
                85                  90                  95      


Asp Ala Tyr Lys Pro Thr Lys Gly Gly Ser Gly Gly Ser Gly Ala Leu 
            100                 105                 110         


Leu Asp Glu Leu Glu Leu Pro Tyr Ala His Glu Gln Leu Trp Arg Phe 
        115                 120                 125             


Leu Asp Ala Pro Val Val Ala Asp Ala Trp Glu Glu Asp Thr Glu Ser 
    130                 135                 140                 


Val Ile Ile Val Glu Thr Ala Thr Val Cys Ala Ala Ile Asp Ser Ala 
145                 150                 155                 160 


Asp Ala Ala Leu Lys Thr Ala Pro Val Val Leu Arg Asp Met Arg Leu 
                165                 170                 175     


Ala Ile Gly Ile Ala Gly Lys Ala Phe Phe Thr Leu Thr Gly Glu Leu 
            180                 185                 190         


Ala Asp Val Glu Ala Ala Ala Glu Val Val Arg Glu Arg Cys Gly Ala 
        195                 200                 205             


Arg Leu Leu Glu Leu Ala Cys Ile Ala Arg Pro Val Asp Glu Leu Arg 
    210                 215                 220                 


Gly Arg Leu Phe Phe 
225                 


<210>  119
<211>  2178
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HcKan_T-YPT31 plasmid

<400>  119
aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt gcagtccagt tacgctggag       60

tccgtctcgt agcgagatat tttgcagcag ttgcgcactt gcatgtgaat gactcttctc      120

ccctttaatt ctgtgctata tttttacaat tttctgctga catatagttt atatacatat      180

agaacgcata taggaaattg aagtaaacag aatacacaag tagaggccgg tatgtacgac      240

attttgctta ctactcttta aaatcatcgt cttcttcgtc ttcatcgtct tcttcttttt      300

caccatatcc tacatcatct ttagagcctg tgctaggttc cttcttgtct aattcttctg      360

cagtcttttt atagtcaatt actttgccgc gtgttcttct tccggatgtg atgatattag      420

aggtatcaat ttctgccaaa tcgtcctctt cttcttctcc ctcatttccc atcaatgcgt      480

ctaacttggc atcgtccata tcagacctcc gagacgactg accatttaaa tcatacctga      540

cctccatagc agaaagtcaa aagcctccga ccggaggctt ttgacttgat cggcacgtaa      600

gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat      660

cgagattttc aggagctaag gaagctaaaa tgagccatat tcaacgggaa acgtcttgct      720

cgaggccgcg attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg      780

ataatgtcgg gcaatcaggt gcgacaatct atcgattgta tgggaagccc gatgcgccag      840

agttgtttct gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca      900

ggctaaactg gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc      960

ctgatgatgc atggttactc accactgcga tcccagggaa aacagcattc caggtattag     1020

aagaatatcc tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt     1080

tgcattcgat tcctgtttgt aattgtcctt ttaacggcga tcgcgtattt cgtctcgctc     1140

aggcgcaatc acgaatgaat aacggtttgg ttggtgcgag tgattttgat gacgagcgta     1200

atggctggcc tgttgaacaa gtctggaaag aaatgcataa gcttttgcca ttctcaccgg     1260

attcagtcgt cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat     1320

taataggttg tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca     1380

tcctatggaa ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat     1440

atggtattga taatcctgat atgaataaat tgcagtttca cttgatgctc gatgagtttt     1500

tctaatgagg gcccaaatgt aatcacctgg ctcaccttcg ggtgggcctt tctgcgttgc     1560

tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga tgctcaagtc     1620

agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc     1680

tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt     1740

cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg     1800

ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat     1860

ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag     1920

ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt     1980

ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc     2040

cagttacctc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     2100

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     2160

tcctttgatt ttctaccg                                                   2178


<210>  120
<211>  5404
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGAH-YPRCd15 plasmid

<400>  120
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc       60

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact      120

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct      180

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag      240

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca      300

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa      360

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc      420

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag      480

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg      540

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca      600

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc      660

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag      720

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata      780

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat      840

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg      900

ggagggctta ccatctggcc ccagtgctgc aatgataccg cggctcccac gctcaccggc      960

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     1020

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     1080

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     1140

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     1200

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     1260

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     1320

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     1380

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     1440

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     1500

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     1560

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     1620

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     1680

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     1740

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ggtctctgtc     1800

agataacgcc aggcgccttt atatcatata attaagacac aaaaggataa aacaaaggtg     1860

ttaactattc tgcatactca ctatcgtaaa ctgtcctgca aatcgtgtaa atatgtattt     1920

catttttttt gcagtgaaaa aaggcatgta aaataccgca tcaagtaact ctactccgcc     1980

tgtggtttca agactaacgg cttgagacaa aatgggaaga aatgattgca gaaaagccat     2040

atgtgtaata gcaaaaagct ggatactgct taccagatgt ttaccttaat ttcttggtga     2100

attagagaag tacagaagtt ttactattaa tcccaccata gaaatttgta taggaaagta     2160

gtttattgga gttattggat atactgtgta aactatttct tgaaattgta atcttaagat     2220

gctcttctta ttctattaaa aatagaaaat gattttcata tttatttatt tatttatatt     2280

ttggcattac tcttcatcat ttttttccct ctaagaagct tcctttcttt ttataaggat     2340

aacaaaacca aaaggaatat tgggtcagat gaatggacgc gaatgcaaga cagaagtcca     2400

aatcacgtca agacaaagaa agaaagaaag aaaaactaac acattaatgt agttttaaaa     2460

tttcaaatcc gaacaacaga gcatagggtt tcgcaaatct ctacctggct cgaagcagcg     2520

gtatttcaca ccgcatagat ccgtcgagtt caagagaaaa aaaaagaaaa agcaaaaaga     2580

aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga atgatgcatt accttgtcat     2640

cttcagtatc atactgttcg tatacatact tactgacatt cataggtata catatataca     2700

catgtatata tatcgtatgc tgcagcttta aataatcggt gtcactacat aagaacacct     2760

ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg cttctcttat ggcaaccgca     2820

agagccttga acgcactctc actacggtga tgatcattct tgcctcgcag acaatcaacg     2880

tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa aatgcgggat catctcgcaa     2940

gagagatctc ctactttctc cctttgcaaa ccaagttcga caactgcgta cggcctgttc     3000

gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag gcgcaaatcc tgatccaaac     3060

ctttttactc cacgcacggc ccctagggcc tctttaaaag cttgaccgag agcaatcccg     3120

cagtcttcag tggtgtgatg gtcgtctatg tgtaagtcac caatgcactc aacgattagc     3180

gaccagccgg aatgcttggc cagagcatgt atcatatggt ccagaaaccc tatacctgtg     3240

tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct     3300

gggaagatcg agtgctctat cgctagggga ccacccttta aagagatcgc aatctgaatc     3360

ttggtttcat ttgtaatacg ctttactagg gctttctgct ctgtcatctt tgccttcgtt     3420

tatcttgcct gctcattttt tagtatattc ttcgaagaaa tcacattact ttatataatg     3480

tataattcat tatgtgataa tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg     3540

gaaaaaaaaa aatgaaaatc attaccgagg cataaaaaaa tatagagtgt actagaggag     3600

gccaagagta atagaaaaag aaaattgcgg gaaaggactg tgttatgact tccctgtgca     3660

ccacctcagg cagagaacct agagacggca atacgcaaac cgcctctccc cgcgcgttgg     3720

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc     3780

aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt     3840

ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag     3900

aggagaaata ctagatggct tcctccgaag acgttatcaa agagttcatg cgtttcaaag     3960

ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc     4020

gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt ccgctgccgt     4080

tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac gttaaacacc     4140

cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg     4200

ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg     4260

gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac ggtccggtta     4320

tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg gaagacggtg     4380

ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac tacgacgctg     4440

aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct tacaaaaccg     4500

acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa cagtacgaac     4560

gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag tgtagatcgc     4620

tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt     4680

tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctccg tctcatgagc     4740

gcttggaagg tcgggatgag catatacaag cactaagaag aacaatacag aactctacac     4800

ggtattattg tgctacaagc tcgagtaaaa ccgagtgttt tgacgatact aacgttgtta     4860

agaaagtaac ttgttatcaa actcattacc aacttgtgat taattggtga ataatatgat     4920

aattgtcgaa attccattgt tggtaaagcc tataatatta tgtatacaga ttatactaga     4980

aattctctcg agaatataag aatccccaaa attgaatcgg tatttctaca tactaatatt     5040

accattactt ctcctttcgt tttatatgtt tcattcctat tacattatcg atctttgcat     5100

ttcagcttcc attatatttg atgtctgttt tatgtcccca cgttacaccg catgtgacag     5160

tatactagta acatgagtgc taccgaatag atgacatttt agactttcat tccaacaact     5220

tggttgacag aatgttacgt accctatatc taatctatat gaggcctgaa tctaactgaa     5280

aggtggaatt tcagtaattt atcaagcttt aataagtttg ggtagtttaa ctgtgcaaaa     5340

aggtatttac cttacatact gaatcttgtc tgtttggtag cggctgcttt atgtcggaga     5400

gacc                                                                  5404


<210>  121
<211>  6264
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pGAH-YPRCd15-GFP-SpyCatcher plasmid

<400>  121
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc       60

ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact      120

ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct      180

gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag      240

ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca      300

cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa      360

cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc      420

gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag      480

aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg      540

tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca      600

gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc      660

tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag      720

gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata      780

tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat      840

ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg      900

ggagggctta ccatctggcc ccagtgctgc aatgataccg cggctcccac gctcaccggc      960

tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc     1020

aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc     1080

gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc     1140

gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc     1200

ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa     1260

gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat     1320

gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata     1380

gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca     1440

tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag     1500

gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc     1560

agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc     1620

aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata     1680

ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta     1740

gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ggtctctgtc     1800

agataacgcc aggcgccttt atatcatata attaagacac aaaaggataa aacaaaggtg     1860

ttaactattc tgcatactca ctatcgtaaa ctgtcctgca aatcgtgtaa atatgtattt     1920

catttttttt gcagtgaaaa aaggcatgta aaataccgca tcaagtaact ctactccgcc     1980

tgtggtttca agactaacgg cttgagacaa aatgggaaga aatgattgca gaaaagccat     2040

atgtgtaata gcaaaaagct ggatactgct taccagatgt ttaccttaat ttcttggtga     2100

attagagaag tacagaagtt ttactattaa tcccaccata gaaatttgta taggaaagta     2160

gtttattgga gttattggat atactgtgta aactatttct tgaaattgta atcttaagat     2220

gctcttctta ttctattaaa aatagaaaat gattttcata tttatttatt tatttatatt     2280

ttggcattac tcttcatcat ttttttccct ctaagaagct tcctttcttt ttataaggat     2340

aacaaaacca aaaggaatat tgggtcagat gaatggacgc gaatgcaaga cagaagtcca     2400

aatcacgtca agacaaagaa agaaagaaag aaaaactaac acattaatgt agttttaaaa     2460

tttcaaatcc gaacaacaga gcatagggtt tcgcaaatct ctacctggct cgaagcagcg     2520

gtatttcaca ccgcatagat ccgtcgagtt caagagaaaa aaaaagaaaa agcaaaaaga     2580

aaaaaggaaa gcgcgcctcg ttcagaatga cacgtataga atgatgcatt accttgtcat     2640

cttcagtatc atactgttcg tatacatact tactgacatt cataggtata catatataca     2700

catgtatata tatcgtatgc tgcagcttta aataatcggt gtcactacat aagaacacct     2760

ttggtggagg gaacatcgtt ggtaccattg ggcgaggtgg cttctcttat ggcaaccgca     2820

agagccttga acgcactctc actacggtga tgatcattct tgcctcgcag acaatcaacg     2880

tggagggtaa ttctgctagc ctctgcaaag ctttcaagaa aatgcgggat catctcgcaa     2940

gagagatctc ctactttctc cctttgcaaa ccaagttcga caactgcgta cggcctgttc     3000

gaaagatcta ccaccgctct ggaaagtgcc tcatccaaag gcgcaaatcc tgatccaaac     3060

ctttttactc cacgcacggc ccctagggcc tctttaaaag cttgaccgag agcaatcccg     3120

cagtcttcag tggtgtgatg gtcgtctatg tgtaagtcac caatgcactc aacgattagc     3180

gaccagccgg aatgcttggc cagagcatgt atcatatggt ccagaaaccc tatacctgtg     3240

tggacgttaa tcacttgcga ttgtgtggcc tgttctgcta ctgcttctgc ctctttttct     3300

gggaagatcg agtgctctat cgctagggga ccacccttta aagagatcgc aatctgaatc     3360

ttggtttcat ttgtaatacg ctttactagg gctttctgct ctgtcatctt tgccttcgtt     3420

tatcttgcct gctcattttt tagtatattc ttcgaagaaa tcacattact ttatataatg     3480

tataattcat tatgtgataa tgccaatcgc taagaaaaaa aaagagtcat ccgctaggtg     3540

gaaaaaaaaa aatgaaaatc attaccgagg cataaaaaaa tatagagtgt actagaggag     3600

gccaagagta atagaaaaag aaaattgcgg gaaaggactg tgttatgact tccctgtgca     3660

ccacctcagg cagagaacct ggctgtgatg tctaagtaac ctttatggta tatttcttaa     3720

tgtggaaaga tactagcgcg cgcacccaca cacaagcttc gtcttttctt gaagaaaaga     3780

ggaagctcgc taaatgggat tccactttcc gttccctgcc agctgatgga aaaaggttag     3840

tggaacgatg aagaataaaa agagagatcc actgaggtga aatttcagct gacagcgagt     3900

ttcatgatcg tgatgaacaa tggtaacgag ttgtggctgt tgccagggag ggtggttctc     3960

aacttttaat gtatggccaa atcgctactt gggtttgtta tataacaaag aagaaataat     4020

gaactgattc tcttcctcct tcttgtcctt tcttaattct gttgtaatta ccttcctttg     4080

taattttttt tgtaattatt cttcttaata atccaaacaa acacacatat tacaatagat     4140

gggttcttct catcatcacc atcaccattc ttctgggatg tctaaaggtg aagaattatt     4200

cactggtgtt gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc     4260

tgtctccggt gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg     4320

tactactggt aaattgccag ttccatggcc aaccttagtc actactttaa cttatggtgt     4380

tcaatgtttt tctagatacc cagatcatat gaaacaacat gactttttca agtctgccat     4440

gccagaaggt tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac     4500

cagagctgaa gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat     4560

tgattttaaa gaagatggta acattttagg tcacaaattg gaatacaact ataactctca     4620

caatgtttac atcatggctg acaaacaaaa gaatggtatc aaagttaact tcaaaattag     4680

acacaacatt gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat     4740

tggtgatggt ccagtcttgt taccagacaa ccattactta tccactcaat ctaaattatc     4800

caaagatcca aacgaaaaga gagatcacat ggtcttgtta gaatttgtta ctgctgctgg     4860

tattacccat ggtatggatg aattgtacaa aggttctggt ggttctgatt ctgctactca     4920

tattaagttc tccaagaggg acgaagatgg taaagaattg gctggtgcaa ctatggaatt     4980

gagagattct tctggtaaga ccatttccac ctggatttct gatggtcaag ttaaggattt     5040

ctacttgtac ccaggtaagt acactttcgt tgaaactgct gctccagatg gttatgaagt     5100

tgctactgct attactttca ccgtcaatga acaaggtcaa gtcactgtta atggttagcg     5160

agatattttg cagcagttgc gcacttgcat gtgaatgact cttctcccct ttaattctgt     5220

gctatatttt tacaattttc tgctgacata tagtttatat acatatagaa cgcatatagg     5280

aaattgaagt aaacagaata cacaagtaga ggccggtatg tacgacattt tgcttactac     5340

tctttaaaat catcgtcttc ttcgtcttca tcgtcttctt ctttttcacc atatcctaca     5400

tcatctttag agcctgtgct aggttccttc ttgtctaatt cttctgcagt ctttttatag     5460

tcaattactt tgccgcgtgt tcttcttccg gatgtgatga tattagaggt atcaatttct     5520

gccaaatcgt cctcttcttc ttctccctca tttcccatca atgcgtctaa cttggcatcg     5580

tccatatcag acctctgagc gcttggaagg tcgggatgag catatacaag cactaagaag     5640

aacaatacag aactctacac ggtattattg tgctacaagc tcgagtaaaa ccgagtgttt     5700

tgacgatact aacgttgtta agaaagtaac ttgttatcaa actcattacc aacttgtgat     5760

taattggtga ataatatgat aattgtcgaa attccattgt tggtaaagcc tataatatta     5820

tgtatacaga ttatactaga aattctctcg agaatataag aatccccaaa attgaatcgg     5880

tatttctaca tactaatatt accattactt ctcctttcgt tttatatgtt tcattcctat     5940

tacattatcg atctttgcat ttcagcttcc attatatttg atgtctgttt tatgtcccca     6000

cgttacaccg catgtgacag tatactagta acatgagtgc taccgaatag atgacatttt     6060

agactttcat tccaacaact tggttgacag aatgttacgt accctatatc taatctatat     6120

gaggcctgaa tctaactgaa aggtggaatt tcagtaattt atcaagcttt aataagtttg     6180

ggtagtttaa ctgtgcaaaa aggtatttac cttacatact gaatcttgtc tgtttggtag     6240

cggctgcttt atgtcggaga gacc                                            6264


