<110>    INDUSTRY FOUNDATION OF CHONNAM NATIONAL UNIVERSITY
         MEDISPAN CO.,Ltd

<120>    Flagellin fusion protein and uses thereof

<130>    OP20-0097/PCT

<150>    KR 10-2020-0136273
<151>    2020-10-20

<160>    90

<170>    KoPatentIn 3.0

<210>    1
<211>    275
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Bacillus subtilis flagellin protein


<400>    1
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg
        275


<210>    2
<211>    505
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Salmonella dublin flagellin protein


<400>    2
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Leu Thr Gln Asn
  1               5                  10                  15 

Asn Leu Asn Lys Ser Gln Ser Ser Leu Ser Ser Ala Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ser Arg Asn Ala Asn Asp Gly Ile Ser Ile Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Asn Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Ser
                 85                  90                  95 

Val Gln Ala Thr Asn Gly Thr Asn Ser Asp Ser Asp Leu Lys Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Gln Gln Arg Leu Glu Glu Ile Asp Arg Val Ser Asn
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Lys Val Leu Ser Gln Asp Asn Gln Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Gly Glu Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Gln Lys Ile Asp Val Lys Ser Leu Gly Leu Asp Gly Phe Asn Val Asn
                165                 170                 175 

Gly Pro Lys Glu Ala Thr Val Gly Asp Leu Lys Ser Ser Phe Lys Asn
            180                 185                 190 

Val Thr Gly Tyr Asp Thr Tyr Ala Ala Gly Ala Asp Lys Tyr Arg Val
        195                 200                 205 

Asp Ile Asn Ser Gly Ala Val Val Thr Asp Ala Val Ala Pro Asp Lys
    210                 215                 220 

Val Tyr Val Asn Ala Ala Asn Gly Gln Leu Thr Thr Asp Asp Ala Glu
225                 230                 235                 240 

Asn Asn Thr Ala Val Asp Leu Phe Lys Thr Thr Lys Ser Thr Ala Gly
                245                 250                 255 

Thr Ala Glu Ala Lys Ala Ile Ala Gly Ala Ile Lys Gly Gly Lys Glu
            260                 265                 270 

Gly Asp Thr Phe Asp Tyr Lys Gly Val Thr Phe Thr Ile Asp Thr Lys
        275                 280                 285 

Thr Gly Asp Asp Gly Asn Gly Lys Val Ser Thr Thr Ile Asn Gly Glu
    290                 295                 300 

Lys Val Thr Leu Thr Val Ala Asp Ile Ala Ile Gly Ala Ala Asp Val
305                 310                 315                 320 

Asn Ala Ala Thr Leu Gln Ser Ser Lys Asn Val Tyr Thr Ser Val Val
                325                 330                 335 

Asn Gly Gln Phe Thr Phe Asp Asp Lys Thr Lys Asn Glu Ser Ala Lys
            340                 345                 350 

Leu Ser Asp Leu Glu Ala Asn Asn Ala Val Lys Gly Glu Ser Lys Ile
        355                 360                 365 

Thr Val Asn Gly Ala Glu Tyr Thr Ala Asn Ala Thr Gly Asp Lys Ile
    370                 375                 380 

Thr Leu Ala Gly Lys Thr Met Phe Ile Asp Lys Thr Ala Ser Gly Val
385                 390                 395                 400 

Ser Thr Leu Ile Asn Glu Asp Ala Ala Ala Ala Lys Lys Ser Thr Ala
                405                 410                 415 

Asn Pro Leu Ala Ser Ile Asp Ser Ala Leu Ser Lys Val Asp Ala Val
            420                 425                 430 

Arg Ser Ser Leu Gly Ala Ile Gln Asn Arg Phe Asp Ser Ala Ile Thr
        435                 440                 445 

Asn Leu Gly Asn Thr Val Thr Asn Leu Asn Ser Ala Arg Ser Arg Ile
    450                 455                 460 

Glu Asp Ala Asp Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln
465                 470                 475                 480 

Ile Leu Gln Gln Ala Gly Thr Ser Val Leu Ala Gln Ala Asn Gln Val
                485                 490                 495 

Pro Gln Asn Val Leu Ser Leu Leu Arg
            500                 505


<210>    3
<211>    387
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Pseudomonas aeruginosa flagellin protein


<400>    3
Met Ala Leu Thr Val Asn Thr Asn Ile Ala Ser Leu Asn Thr Gln Arg
  1               5                  10                  15 

Asn Gln Asn Asn Ser Ser Ala Ser Leu Asn Thr Ser Leu Gln Arg Leu
             20                  25                  30 

Ser Thr Gly Ser Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Leu
         35                  40                  45 

Gln Ile Ala Asn Arg Leu Thr Ser Gln Val Asn Gly Leu Asn Val Ala
     50                  55                  60 

Thr Lys Asn Ala Asn Asp Gly Ile Ser Leu Ala Gln Thr Ala Glu Gly
 65                  70                  75                  80 

Ala Leu Gln Gln Ser Thr Asn Ile Leu Gln Arg Met Arg Asp Leu Ser
                 85                  90                  95 

Leu Gln Ser Ala Asn Gly Ser Asn Ser Asp Ser Glu Arg Thr Ala Leu
            100                 105                 110 

Asn Gly Glu Val Lys Gln Leu Gln Lys Glu Leu Asp Arg Ile Ser Asn
        115                 120                 125 

Thr Thr Thr Phe Gly Gly Arg Lys Leu Leu Asp Gly Ser Phe Gly Val
    130                 135                 140 

Ala Ser Phe Gln Val Gly Ser Ala Ala Asn Glu Ile Ile Ser Val Gly
145                 150                 155                 160 

Ile Asp Glu Met Ser Ala Glu Ser Leu Asn Gly Thr Tyr Phe Lys Ala
                165                 170                 175 

Asp Gly Gly Gly Ala Val Thr Ala Ala Thr Ala Ser Gly Thr Val Asp
            180                 185                 190 

Ile Ala Ile Asp Ile Thr Gly Gly Ser Ala Val Asn Val Lys Val Asp
        195                 200                 205 

Met Lys Gly Asn Glu Thr Ala Glu Gln Ala Ala Ala Lys Ile Ala Ala
    210                 215                 220 

Ala Val Asn Asp Ala Asn Val Gly Ile Gly Ala Phe Thr Asp Gly Ala
225                 230                 235                 240 

Gln Ile Ser Tyr Val Ser Lys Ala Ser Ala Asp Gly Thr Thr Ser Ala
                245                 250                 255 

Val Ser Gly Val Ala Ile Thr Asp Thr Gly Ser Thr Gly Ala Gly Thr
            260                 265                 270 

Ala Ala Gly Thr Thr Thr Phe Thr Glu Ala Asn Asp Thr Val Ala Lys
        275                 280                 285 

Ile Asp Ile Ser Thr Ala Lys Gly Ala Gln Ser Ala Val Leu Val Ile
    290                 295                 300 

Asp Glu Ala Ile Lys Gln Ile Asp Ala Gln Arg Ala Asp Leu Gly Ala
305                 310                 315                 320 

Val Gln Asn Arg Phe Asp Asn Thr Ile Asn Asn Leu Lys Asn Ile Gly
                325                 330                 335 

Glu Asn Val Ser Ala Ala Arg Gly Arg Ile Glu Asp Thr Asp Phe Ala
            340                 345                 350 

Ala Glu Thr Ala Asn Leu Thr Lys Asn Gln Val Leu Gln Gln Ala Gly
        355                 360                 365 

Thr Ala Ile Leu Ala Gln Ala Asn Gln Leu Pro Gln Ser Val Leu Ser
    370                 375                 380 

Leu Leu Arg
385        


<210>    4
<211>    550
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Shigella flexneri flagellin protein


<400>    4
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Ile Thr Gln Asn
  1               5                  10                  15 

Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser Ser Ser Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ala Arg Asn Ala Asn Asp Gly Ile Ser Val Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln Arg Ile Arg Glu Leu Thr
                 85                  90                  95 

Val Gln Ala Ser Thr Gly Thr Asn Ser Asp Ser Asp Leu Asp Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu Ile Asp Arg Val Ser Gly
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Asn Val Leu Ala Lys Asp Gly Ser Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Gly Gln Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Lys Lys Ile Asp Ser Asp Thr Leu Gly Leu Asn Gly Phe Asn Val Asn
                165                 170                 175 

Gly Gly Gly Ala Val Ala Asn Thr Ala Ala Ser Lys Ala Asp Leu Val
            180                 185                 190 

Ala Ala Asn Ala Thr Val Val Gly Asn Lys Tyr Thr Val Ser Ala Gly
        195                 200                 205 

Tyr Asp Ala Ala Lys Ala Ser Asp Leu Leu Ala Gly Val Ser Asp Gly
    210                 215                 220 

Asp Thr Val Gln Ala Thr Ile Asn Asn Gly Phe Gly Thr Ala Ala Ser
225                 230                 235                 240 

Ala Thr Asn Tyr Lys Tyr Asp Ser Ala Ser Lys Ser Tyr Ser Phe Asp
                245                 250                 255 

Thr Thr Thr Ala Ser Ala Ala Asp Val Gln Lys Tyr Leu Thr Pro Gly
            260                 265                 270 

Val Gly Asp Thr Ala Lys Gly Thr Ile Thr Ile Asp Gly Ser Ala Gln
        275                 280                 285 

Asp Val Gln Ile Ser Ser Asp Gly Lys Ile Thr Ala Ser Asn Gly Asp
    290                 295                 300 

Lys Leu Tyr Ile Asp Thr Thr Gly Arg Leu Thr Lys Asn Gly Ser Gly
305                 310                 315                 320 

Ala Ser Leu Thr Glu Ala Ser Leu Ser Thr Leu Ala Ala Asn Asn Thr
                325                 330                 335 

Lys Ala Thr Thr Ile Asp Ile Gly Gly Thr Ser Ile Ser Phe Thr Gly
            340                 345                 350 

Asn Ser Thr Thr Pro Asp Thr Ile Thr Tyr Ser Val Thr Gly Ala Lys
        355                 360                 365 

Val Asp Gln Ala Ala Phe Asp Lys Ala Val Ser Thr Ser Gly Asn Asn
    370                 375                 380 

Val Asp Phe Thr Thr Ala Gly Tyr Ser Val Asn Gly Thr Thr Gly Ala
385                 390                 395                 400 

Val Thr Lys Gly Val Asp Ser Val Tyr Val Asp Asn Asn Glu Ala Leu
                405                 410                 415 

Thr Thr Ser Asp Thr Val Asp Phe Tyr Leu Gln Asp Asp Gly Ser Val
            420                 425                 430 

Thr Asn Gly Ser Gly Lys Ala Val Tyr Lys Asp Ala Asp Gly Lys Leu
        435                 440                 445 

Thr Thr Asp Ala Glu Thr Lys Ala Ala Thr Thr Ala Asp Pro Leu Lys
    450                 455                 460 

Ala Leu Asp Glu Ala Ile Ser Ser Ile Asp Lys Phe Arg Ser Ser Leu
465                 470                 475                 480 

Gly Ala Val Gln Asn Arg Leu Asp Ser Ala Val Thr Asn Leu Asn Asn
                485                 490                 495 

Thr Thr Thr Asn Leu Ser Glu Ala Gln Ser Arg Ile Gln Asp Ala Asp
            500                 505                 510 

Tyr Ala Thr Glu Val Ser Asn Met Ser Lys Ala Gln Ile Ile Gln Gln
        515                 520                 525 

Ala Gly Asn Ser Val Leu Ala Lys Ala Asn Gln Val Pro Gln Gln Val
    530                 535                 540 

Leu Ser Leu Leu Gln Gly
545                 550


<210>    5
<211>    498
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Escherichia coli flagellin protein


<400>    5
Met Ala Gln Val Ile Asn Thr Asn Ser Leu Ser Leu Ile Thr Gln Asn
  1               5                  10                  15 

Asn Ile Asn Lys Asn Gln Ser Ala Leu Ser Ser Ser Ile Glu Arg Leu
             20                  25                  30 

Ser Ser Gly Leu Arg Ile Asn Ser Ala Lys Asp Asp Ala Ala Gly Gln
         35                  40                  45 

Ala Ile Ala Asn Arg Phe Thr Ser Asn Ile Lys Gly Leu Thr Gln Ala
     50                  55                  60 

Ala Arg Asn Ala Asn Asp Gly Ile Ser Val Ala Gln Thr Thr Glu Gly
 65                  70                  75                  80 

Ala Leu Ser Glu Ile Asn Asn Asn Leu Gln Arg Val Arg Glu Leu Thr
                 85                  90                  95 

Val Gln Ala Thr Thr Gly Thr Asn Ser Glu Ser Asp Leu Ser Ser Ile
            100                 105                 110 

Gln Asp Glu Ile Lys Ser Arg Leu Asp Glu Ile Asp Arg Val Ser Gly
        115                 120                 125 

Gln Thr Gln Phe Asn Gly Val Asn Val Leu Ala Lys Asn Gly Ser Met
    130                 135                 140 

Lys Ile Gln Val Gly Ala Asn Asp Asn Gln Thr Ile Thr Ile Asp Leu
145                 150                 155                 160 

Lys Gln Ile Asp Ala Lys Thr Leu Gly Leu Asp Gly Phe Ser Val Lys
                165                 170                 175 

Asn Asn Asp Thr Val Thr Thr Ser Ala Pro Val Thr Ala Phe Gly Ala
            180                 185                 190 

Thr Thr Thr Asn Asn Ile Lys Leu Thr Gly Ile Thr Leu Ser Thr Glu
        195                 200                 205 

Ala Ala Thr Asp Thr Gly Gly Thr Asn Pro Ala Ser Ile Glu Gly Val
    210                 215                 220 

Tyr Thr Asp Asn Gly Asn Asp Tyr Tyr Ala Lys Ile Thr Gly Gly Asp
225                 230                 235                 240 

Asn Asp Gly Lys Tyr Tyr Ala Val Thr Val Ala Asn Asp Gly Thr Val
                245                 250                 255 

Thr Met Ala Thr Gly Ala Thr Ala Asn Ala Thr Val Thr Asp Ala Asn
            260                 265                 270 

Thr Thr Lys Ala Thr Thr Ile Thr Ser Gly Gly Thr Pro Val Gln Ile
        275                 280                 285 

Asp Asn Thr Ala Gly Ser Ala Thr Ala Asn Leu Gly Ala Val Ser Leu
    290                 295                 300 

Val Lys Leu Gln Asp Ser Lys Gly Asn Asp Thr Asp Thr Tyr Ala Leu
305                 310                 315                 320 

Lys Asp Thr Asn Gly Asn Leu Tyr Ala Ala Asp Val Asn Glu Thr Thr
                325                 330                 335 

Gly Ala Val Ser Val Lys Thr Ile Thr Tyr Thr Asp Ser Ser Gly Ala
            340                 345                 350 

Ala Ser Ser Pro Thr Ala Val Lys Leu Gly Gly Asp Asp Gly Lys Thr
        355                 360                 365 

Glu Val Val Asp Ile Asp Gly Lys Thr Tyr Asp Ser Ala Asp Leu Asn
    370                 375                 380 

Gly Gly Asn Leu Gln Thr Gly Leu Thr Ala Gly Gly Glu Ala Leu Thr
385                 390                 395                 400 

Ala Val Ala Asn Gly Lys Thr Thr Asp Pro Leu Lys Ala Leu Asp Asp
                405                 410                 415 

Ala Ile Ala Ser Val Asp Lys Phe Arg Ser Ser Leu Gly Ala Val Gln
            420                 425                 430 

Asn Arg Leu Asp Ser Ala Val Thr Asn Leu Asn Asn Thr Thr Thr Asn
        435                 440                 445 

Leu Ser Glu Ala Gln Ser Arg Ile Gln Asp Ala Asp Tyr Ala Thr Glu
    450                 455                 460 

Val Ser Asn Met Ser Lys Ala Gln Ile Ile Gln Gln Ala Gly Asn Ser
465                 470                 475                 480 

Val Leu Ala Lys Ala Asn Gln Val Pro Gln Gln Val Leu Ser Leu Leu
                485                 490                 495 

Gln Gly
       


<210>    6
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant - PGK


<400>    6
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Pro Gly Lys
225                


<210>    7
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant + S220P (PGK)


<400>    7
Glu Pro Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Pro Gly Lys
225                


<210>    8
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant + G223T (PGK)


<400>    8
Glu Ser Lys Tyr Thr Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Pro Gly Lys
225                


<210>    9
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant + P224H (PGK)


<400>    9
Glu Ser Lys Tyr Gly His Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Pro Gly Lys
225                


<210>    10
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant + P225T


<400>    10
Glu Ser Lys Tyr Gly Pro Thr Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Pro Gly Lys
225                


<210>    11
<211>    229
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    hIgG4 Fc S228P mutant - LGK


<400>    11
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe
  1               5                  10                  15 

Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr
             20                  25                  30 

Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val
         35                  40                  45 

Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val
     50                  55                  60 

Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser
 65                  70                  75                  80 

Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu
                 85                  90                  95 

Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser
            100                 105                 110 

Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro
        115                 120                 125 

Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln
    130                 135                 140 

Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala
145                 150                 155                 160 

Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr
                165                 170                 175 

Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu
            180                 185                 190 

Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser
        195                 200                 205 

Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser
    210                 215                 220 

Leu Ser Leu Gly Lys
225                


<210>    12
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Linker 1


<400>    12
Gly Gly Gly Gly Ser
  1               5


<210>    13
<211>    15
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Linker 2


<400>    13
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
  1               5                  10                  15


<210>    14
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Forward primer


<400>    14
ccggatatcg atgagaatta accacaatat tgcagcactt aac                           43


<210>    15
<211>    54
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Reverse primer


<400>    15
gaccatggca gaccctccgc caccacgtaa taattgaagt acgttttgag gctg               54


<210>    16
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Forward primer


<400>    16
ccggatatcg atgagaatta accacaatat tgcagcactt aac                           43


<210>    17
<211>    84
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Reverse primer


<400>    17
gaccatggca gaccctccgc caccagaccc tccgccacca gaccctccgc caccacgtaa         60

taattgaagt acgttttgag gctg                                                84


<210>    18
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Forward primer


<400>    18
ccggatatcg atgagaatta accacaatat tgcagcactt aac                           43


<210>    19
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge Reverse primer


<400>    19
tcagatctaa ccatggcacg taataattga ag                                       32


<210>    20
<211>    48
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC Forward primer


<400>    20
tatatccatg gttagatctg aatccaaata cggtccccca tgcccatc                      48


<210>    21
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC Reverse primer


<400>    21
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    22
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC Forward primer


<400>    22
ccggatatcg atgagaatta accacaatat tgcagcactt aac                           43


<210>    23
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC Reverse primer


<400>    23
tcagatctaa ccatggcacg taataattga ag                                       32


<210>    24
<211>    63
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge Forward primer


<400>    24
tatatccatg gttagatctg aatccaaata cggtccccca tgcccacctt gcccagcacc         60

tga                                                                       63


<210>    25
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge Reverse primer


<400>    25
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    26
<211>    43
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC Forward primer


<400>    26
ccggatatcg atgagaatta accacaatat tgcagcactt aac                           43


<210>    27
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC Reverse primer


<400>    27
tcagatctaa ccatggcacg taataattga ag                                       32


<210>    28
<211>    57
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-S220P Forward primer


<400>    28
ggagggtctg ccatggttag atctgaaccc aaatacggtc ccccatgccc accttgc            57


<210>    29
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-S220P Reverse primer


<400>    29
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    30
<211>    57
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-G223T Forward primer


<400>    30
ggagggtctg ccatggttag atctgaatcc aaatacactc ccccatgccc accttgc            57


<210>    31
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-G223T Reverse primer


<400>    31
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    32
<211>    57
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P224H Forward primer


<400>    32
ggagggtctg ccatggttag atctgaatcc aaatacggtc acccatgccc accttgc            57


<210>    33
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P224H Reverse primer


<400>    33
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    34
<211>    57
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P225T Forward primer


<400>    34
ggagggtctg ccatggttag atctgaatcc aaatacggtc ccacatgccc accttgc            57


<210>    35
<211>    37
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P225T Reverse primer


<400>    35
tatatgctag cactcattta cccagagaca gggagag                                  37


<210>    36
<211>    48
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306L) Forward
         primer


<400>    36
gacaaggata tcgatgagaa ttaaccacaa tattgcagca cttaacac                      48


<210>    37
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306L) Reverse
         primer


<400>    37
gacgctagct catttaccca gagacaggga gaggc                                    35


<210>    38
<211>    1530
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge (MSP303) DNA


<400>    38
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctccccc atgcccatca tgcccagcac ctgagttcct ggggggacca        900

tcagtcttcc tgttcccccc aaaacccaag gacactctca tgatctcccg gacccctgag        960

gtcacgtgcg tggtggtgga cgtgagccag gaagaccccg aggtccagtt caactggtac       1020

gtggatggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gttcaacagc       1080

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa cggcaaggag       1140

tacaagtgca aggtctccaa caaaggcctc ccgtcctcca tcgagaaaac catctccaaa       1200

gccaaagggc agccccgaga gccacaggtg tacaccctgc ccccatccca ggaggagatg       1260

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc       1320

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg       1380

gactccgacg gctccttctt cctctacagc aggctaaccg tggacaagag caggtggcag       1440

gaggggaatg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag       1500

aagagcctct ccctgtctcc gggtaaatga                                        1530


<210>    39
<211>    509
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge (MSP303) Protein


<400>    39
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Pro Pro Cys
        275                 280                 285 

Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu
    290                 295                 300 

Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
305                 310                 315                 320 

Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln
                325                 330                 335 

Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
            340                 345                 350 

Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
        355                 360                 365 

Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
    370                 375                 380 

Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys
385                 390                 395                 400 

Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
                405                 410                 415 

Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
            420                 425                 430 

Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
        435                 440                 445 

Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
    450                 455                 460 

Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln
465                 470                 475                 480 

Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
                485                 490                 495 

His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            500                 505                


<210>    40
<211>    1560
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge (MSP304) DNA


<400>    40
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

ggtggcggag ggtctggtgg cggagggtct gccatggtta gatctccccc atgcccatca        900

tgcccagcac ctgagttcct ggggggacca tcagtcttcc tgttcccccc aaaacccaag        960

gacactctca tgatctcccg gacccctgag gtcacgtgcg tggtggtgga cgtgagccag       1020

gaagaccccg aggtccagtt caactggtac gtggatggcg tggaggtgca taatgccaag       1080

acaaagccgc gggaggagca gttcaacagc acgtaccgtg tggtcagcgt cctcaccgtc       1140

ctgcaccagg actggctgaa cggcaaggag tacaagtgca aggtctccaa caaaggcctc       1200

ccgtcctcca tcgagaaaac catctccaaa gccaaagggc agccccgaga gccacaggtg       1260

tacaccctgc ccccatccca ggaggagatg accaagaacc aggtcagcct gacctgcctg       1320

gtcaaaggct tctaccccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag       1380

aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc       1440

aggctaaccg tggacaagag caggtggcag gaggggaatg tcttctcatg ctccgtgatg       1500

catgaggctc tgcacaacca ctacacacag aagagcctct ccctgtctcc gggtaaatga       1560

                                                                        1560


<210>    41
<211>    519
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge (MSP304) Protein


<400>    41
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly
        275                 280                 285 

Gly Ser Ala Met Val Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala Pro
    290                 295                 300 

Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys
305                 310                 315                 320 

Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val
                325                 330                 335 

Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp
            340                 345                 350 

Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe
        355                 360                 365 

Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp
    370                 375                 380 

Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu
385                 390                 395                 400 

Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg
                405                 410                 415 

Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys
            420                 425                 430 

Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp
        435                 440                 445 

Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys
    450                 455                 460 

Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser
465                 470                 475                 480 

Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser
                485                 490                 495 

Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser
            500                 505                 510 

Leu Ser Leu Ser Pro Gly Lys
        515                


<210>    42
<211>    1515
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge-NL DNA


<400>    42
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtgccat ggttagatct        840

cccccatgcc catcatgccc agcacctgag ttcctggggg gaccatcagt cttcctgttc        900

cccccaaaac ccaaggacac tctcatgatc tcccggaccc ctgaggtcac gtgcgtggtg        960

gtggacgtga gccaggaaga ccccgaggtc cagttcaact ggtacgtgga tggcgtggag       1020

gtgcataatg ccaagacaaa gccgcgggag gagcagttca acagcacgta ccgtgtggtc       1080

agcgtcctca ccgtcctgca ccaggactgg ctgaacggca aggagtacaa gtgcaaggtc       1140

tccaacaaag gcctcccgtc ctccatcgag aaaaccatct ccaaagccaa agggcagccc       1200

cgagagccac aggtgtacac cctgccccca tcccaggagg agatgaccaa gaaccaggtc       1260

agcctgacct gcctggtcaa aggcttctac cccagcgaca tcgccgtgga gtgggagagc       1320

aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc cgacggctcc       1380

ttcttcctct acagcaggct aaccgtggac aagagcaggt ggcaggaggg gaatgtcttc       1440

tcatgctccg tgatgcatga ggctctgcac aaccactaca cacagaagag cctctccctg       1500

tctccgggta aatga                                                        1515


<210>    43
<211>    504
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-partial hinge-NL Protein


<400>    43
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Ala Met Val Arg Ser Pro Pro Cys Pro Ser Cys Pro Ala
        275                 280                 285 

Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro
    290                 295                 300 

Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val
305                 310                 315                 320 

Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val
                325                 330                 335 

Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln
            340                 345                 350 

Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln
        355                 360                 365 

Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly
    370                 375                 380 

Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro
385                 390                 395                 400 

Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr
                405                 410                 415 

Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser
            420                 425                 430 

Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr
        435                 440                 445 

Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr
    450                 455                 460 

Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe
465                 470                 475                 480 

Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys
                485                 490                 495 

Ser Leu Ser Leu Ser Pro Gly Lys
            500                


<210>    44
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC (MSP305) DNA


<400>    44
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cccccatgcc catcatgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    45
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC (MSP305) Protein


<400>    45
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    46
<211>    1530
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC-NL DNA


<400>    46
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtgccat ggttagatct        840

gaatccaaat acggtccccc atgcccatca tgcccagcac ctgagttcct ggggggacca        900

tcagtcttcc tgttcccccc aaaacccaag gacactctca tgatctcccg gacccctgag        960

gtcacgtgcg tggtggtgga cgtgagccag gaagaccccg aggtccagtt caactggtac       1020

gtggatggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gttcaacagc       1080

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa cggcaaggag       1140

tacaagtgca aggtctccaa caaaggcctc ccgtcctcca tcgagaaaac catctccaaa       1200

gccaaagggc agccccgaga gccacaggtg tacaccctgc ccccatccca ggaggagatg       1260

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc       1320

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg       1380

gactccgacg gctccttctt cctctacagc aggctaaccg tggacaagag caggtggcag       1440

gaggggaatg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag       1500

aagagcctct ccccgtctcc gggtaaatga                                        1530


<210>    47
<211>    509
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPSC-NL Protein


<400>    47
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Ala Met Val Arg Ser Glu Ser Lys Tyr Gly Pro Pro Cys
        275                 280                 285 

Pro Ser Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu
    290                 295                 300 

Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
305                 310                 315                 320 

Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln
                325                 330                 335 

Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
            340                 345                 350 

Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
        355                 360                 365 

Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
    370                 375                 380 

Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys
385                 390                 395                 400 

Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
                405                 410                 415 

Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
            420                 425                 430 

Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
        435                 440                 445 

Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
    450                 455                 460 

Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln
465                 470                 475                 480 

Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
                485                 490                 495 

His Tyr Thr Gln Lys Ser Leu Ser Pro Ser Pro Gly Lys
            500                 505                


<210>    48
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306) DNA


<400>    48
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cccccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    49
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306) Protein


<400>    49
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    50
<211>    1530
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-NL DNA


<400>    50
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtgccat ggttagatct        840

gaatccaaat acggtccccc atgcccacct tgcccagcac ctgagttcct ggggggacca        900

tcagtcttcc tgttcccccc aaaacccaag gacactctca tgatctcccg gacccctgag        960

gtcacgtgcg tggtggtgga cgtgagccag gaagaccccg aggtccagtt caactggtac       1020

gtggatggcg tggaggtgca taatgccaag acaaagccgc gggaggagca gttcaacagc       1080

acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa cggcaaggag       1140

tacaagtgca aggtctccaa caaaggcctc ccgtcctcca tcgagaaaac catctccaaa       1200

gccaaagggc agccccgaga gccacaggtg tacaccctgc ccccatccca ggaggagatg       1260

accaagaacc aggtcagcct gacctgcctg gtcaaaggct tctaccccag cgacatcgcc       1320

gtggagtggg agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg       1380

gactccgacg gctccttctt cctctacagc aggctaaccg tggacaagag caggtggcag       1440

gaggggaatg tcttctcatg ctccgtgatg catgaggctc tgcacaacca ctacacacag       1500

aagagcctct ccctgtctcc gggtaaatga                                        1530


<210>    51
<211>    509
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-NL Protein


<400>    51
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Ala Met Val Arg Ser Glu Ser Lys Tyr Gly Pro Pro Cys
        275                 280                 285 

Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu
    290                 295                 300 

Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu
305                 310                 315                 320 

Val Thr Cys Val Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln
                325                 330                 335 

Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys
            340                 345                 350 

Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu
        355                 360                 365 

Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys
    370                 375                 380 

Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys
385                 390                 395                 400 

Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser
                405                 410                 415 

Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys
            420                 425                 430 

Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln
        435                 440                 445 

Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly
    450                 455                 460 

Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln
465                 470                 475                 480 

Glu Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn
                485                 490                 495 

His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys
            500                 505                


<210>    52
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-S220P DNA


<400>    52
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaacc caaatacggt cccccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    53
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-S220P Protein


<400>    53
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Pro Lys
        275                 280                 285 

Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    54
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-G223T DNA


<400>    54
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacact cccccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    55
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-G223T Protein


<400>    55
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Thr Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    56
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P224H DNA


<400>    56
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cacccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    57
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P224H Protein


<400>    57
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly His Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    58
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P225T DNA


<400>    58
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cccacatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctccgggta aatga                       1545


<210>    59
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC-P225T Protein


<400>    59
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly Pro Thr Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro
            500                 505                 510 

Gly Lys
       


<210>    60
<211>    1545
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306L) DNA


<400>    60
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cccccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctctgggta aatga                       1545


<210>    61
<211>    514
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pFUSE-hIgG4-fc2-bsFlagellin-full hinge-CPPC (MSP306L) Protein


<400>    61
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu
            500                 505                 510 

Gly Lys
       


<210>    62
<211>    1590
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    pAcGP67-A-bsFlagellin-full hinge-CPPC (MSP306L-Insect) DNA


<400>    62
atgagaatta accacaatat tgcagcactt aacacattga atcgtttggg ttcaaacaac         60

ggagcagcac aaaagaatat ggagaagctt tcttcaggtc ttcgtatcaa ccgtgcggga        120

gatgacgcag caggtctagc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa        180

atggcttcta aaaactctca agatggaatc tctcttatcc aaacagctga gggtgcatta        240

actgaaactc atgcaattct tcaacgtatg cgtgaactta ctgttcaagc aggaaacaca        300

ggtacacaac aagctgaaga tcttggtgca attaaagatg aaatggatgc gcttatcgag        360

gaaattgatg gcatttcaaa ccgtactgaa tttaacggta aaaagttgct agacggaact        420

aattctactg atggtttcac attccaaatt ggtgcgaatg ctggccaaca actaaatgta        480

aaaattgaca gcatgtcatc aactgcttta ggagtaaacg cacttgatgt aacagatttc        540

gctgctactg cttttgatga tcaacttaaa agtattgata ctgccatcaa tactgtatca        600

actcaacgtg ctaaattagg tgcggtacaa aaccgtctag agcatacaat caacaactta        660

ggcgcttctg gtgaaaacct gacagctgct gagtctcgta tccgtgacgt tgacatggct        720

aaagaaatga gcgagttcac taagaacaac attctttctc aagcttctca agctatgctt        780

gctcaagcta accaacagcc tcaaaacgta cttcaattat tacgtggtgg cggagggtct        840

gccatggtta gatctgaatc caaatacggt cccccatgcc caccttgccc agcacctgag        900

ttcctggggg gaccatcagt cttcctgttc cccccaaaac ccaaggacac tctcatgatc        960

tcccggaccc ctgaggtcac gtgcgtggtg gtggacgtga gccaggaaga ccccgaggtc       1020

cagttcaact ggtacgtgga tggcgtggag gtgcataatg ccaagacaaa gccgcgggag       1080

gagcagttca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg       1140

ctgaacggca aggagtacaa gtgcaaggtc tccaacaaag gcctcccgtc ctccatcgag       1200

aaaaccatct ccaaagccaa agggcagccc cgagagccac aggtgtacac cctgccccca       1260

tcccaggagg agatgaccaa gaaccaggtc agcctgacct gcctggtcaa aggcttctac       1320

cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa ctacaagacc       1380

acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaggct aaccgtggac       1440

aagagcaggt ggcaggaggg gaatgtcttc tcatgctccg tgatgcatga ggctctgcac       1500

aaccactaca cacagaagag cctctccctg tctctgggta aatgcggccg cctggttccg       1560

cgtggttcgc atcatcatca tcatcactga                                        1590


<210>    63
<211>    529
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    pAcGP67-A-bsFlagellin-full hinge-CPPC (MSP306L-Insect) Protein


<400>    63
Met Arg Ile Asn His Asn Ile Ala Ala Leu Asn Thr Leu Asn Arg Leu
  1               5                  10                  15 

Gly Ser Asn Asn Gly Ala Ala Gln Lys Asn Met Glu Lys Leu Ser Ser
             20                  25                  30 

Gly Leu Arg Ile Asn Arg Ala Gly Asp Asp Ala Ala Gly Leu Ala Ile
         35                  40                  45 

Ser Glu Lys Met Arg Gly Gln Ile Arg Gly Leu Glu Met Ala Ser Lys
     50                  55                  60 

Asn Ser Gln Asp Gly Ile Ser Leu Ile Gln Thr Ala Glu Gly Ala Leu
 65                  70                  75                  80 

Thr Glu Thr His Ala Ile Leu Gln Arg Met Arg Glu Leu Thr Val Gln
                 85                  90                  95 

Ala Gly Asn Thr Gly Thr Gln Gln Ala Glu Asp Leu Gly Ala Ile Lys
            100                 105                 110 

Asp Glu Met Asp Ala Leu Ile Glu Glu Ile Asp Gly Ile Ser Asn Arg
        115                 120                 125 

Thr Glu Phe Asn Gly Lys Lys Leu Leu Asp Gly Thr Asn Ser Thr Asp
    130                 135                 140 

Gly Phe Thr Phe Gln Ile Gly Ala Asn Ala Gly Gln Gln Leu Asn Val
145                 150                 155                 160 

Lys Ile Asp Ser Met Ser Ser Thr Ala Leu Gly Val Asn Ala Leu Asp
                165                 170                 175 

Val Thr Asp Phe Ala Ala Thr Ala Phe Asp Asp Gln Leu Lys Ser Ile
            180                 185                 190 

Asp Thr Ala Ile Asn Thr Val Ser Thr Gln Arg Ala Lys Leu Gly Ala
        195                 200                 205 

Val Gln Asn Arg Leu Glu His Thr Ile Asn Asn Leu Gly Ala Ser Gly
    210                 215                 220 

Glu Asn Leu Thr Ala Ala Glu Ser Arg Ile Arg Asp Val Asp Met Ala
225                 230                 235                 240 

Lys Glu Met Ser Glu Phe Thr Lys Asn Asn Ile Leu Ser Gln Ala Ser
                245                 250                 255 

Gln Ala Met Leu Ala Gln Ala Asn Gln Gln Pro Gln Asn Val Leu Gln
            260                 265                 270 

Leu Leu Arg Gly Gly Gly Gly Ser Ala Met Val Arg Ser Glu Ser Lys
        275                 280                 285 

Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe Leu Gly Gly
    290                 295                 300 

Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile
305                 310                 315                 320 

Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser Gln Glu
                325                 330                 335 

Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His
            340                 345                 350 

Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Tyr Arg
        355                 360                 365 

Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys
    370                 375                 380 

Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser Ser Ile Glu
385                 390                 395                 400 

Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr
                405                 410                 415 

Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln Val Ser Leu
            420                 425                 430 

Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp
        435                 440                 445 

Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val
    450                 455                 460 

Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu Thr Val Asp
465                 470                 475                 480 

Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser Val Met His
                485                 490                 495 

Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Leu
            500                 505                 510 

Gly Lys Cys Gly Arg Leu Val Pro Arg Gly Ser His His His His His
        515                 520                 525 

His
   


<210>    64
<211>    2
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 1


<400>    64
Gly Gly
 14    


<210>    65
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 2


<400>    65
Gly Ala Gly
  1        


<210>    66
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 3


<400>    66
Gly Pro Ala
  1        


<210>    67
<211>    1
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 4


<400>    67
Gly
167


<210>    68
<211>    2
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 5


<400>    68
Gly Ser
 18    


<210>    69
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 6


<400>    69
Gly Ser Gly Ser Gly
  1               5


<210>    70
<211>    3
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 7


<400>    70
Gly Gly Ser
  1        


<210>    71
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 8


<400>    71
Gly Gly Ser Ser Gly
  1               5


<210>    72
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 9


<400>    72
Gly Gly Ser Gly
  1            


<210>    73
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 10


<400>    73
Gly Gly Gly Ser
  1            


<210>    74
<211>    6
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 11


<400>    74
Gly Gly Ser Gly Gly Gly
  1               5    


<210>    75
<211>    13
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    peptide linker 12


<400>    75
Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly
  1               5                  10            


<210>    76
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 1


<400>    76
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro
  1               5                  10        


<210>    77
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 2


<400>    77
Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
  1               5                  10        


<210>    78
<211>    11
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 3


<400>    78
Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
  1               5                  10    


<210>    79
<211>    10
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 4


<400>    79
Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
  1               5                  10


<210>    80
<211>    9
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 5


<400>    80
Tyr Gly Pro Pro Cys Pro Pro Cys Pro
  1               5                


<210>    81
<211>    8
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 6


<400>    81
Gly Pro Pro Cys Pro Pro Cys Pro
  1               5            


<210>    82
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 7


<400>    82
Pro Pro Cys Pro Pro Cys Pro
  1               5        


<210>    83
<211>    6
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 8


<400>    83
Pro Cys Pro Pro Cys Pro
  1               5    


<210>    84
<211>    5
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 9


<400>    84
Cys Pro Pro Cys Pro
  1               5


<210>    85
<211>    4
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge 10


<400>    85
Cys Pro Pro Cys
  1            


<210>    86
<211>    7
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge mutant 1


<400>    86
Pro Pro Cys Pro Ser Cys Pro
  1               5        


<210>    87
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge mutant 2


<400>    87
Glu Pro Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
  1               5                  10        


<210>    88
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge mutant 3


<400>    88
Glu Ser Lys Tyr Thr Pro Pro Cys Pro Pro Cys Pro
  1               5                  10        


<210>    89
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge mutant 4


<400>    89
Glu Ser Lys Tyr Gly His Pro Cys Pro Pro Cys Pro
  1               5                  10        


<210>    90
<211>    12
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    IgG4 Fc hinge mutant 5


<400>    90
Glu Ser Lys Tyr Gly Pro Thr Cys Pro Pro Cys Pro
  1               5                  10        
